GB1550753A - Stabilized liquid enzyme reagent compositions - Google Patents
Stabilized liquid enzyme reagent compositionsInfo
- Publication number
- GB1550753A GB1550753A GB1124177A GB1124177A GB1550753A GB 1550753 A GB1550753 A GB 1550753A GB 1124177 A GB1124177 A GB 1124177A GB 1124177 A GB1124177 A GB 1124177A GB 1550753 A GB1550753 A GB 1550753A
- Authority
- GB
- United Kingdom
- Prior art keywords
- composition
- stabilised
- aqueous
- enzymes
- aqueous medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The stable liquid composition comprises molecularly stabilised enzymes which otherwise would be labile and unstable in aqueous medium. The enzymes are stabilised by treating them with a water-miscible organic solvent, for example 30% aqueous propanediol, in the presence of a nonreactive polymer, for example, 0.1% gelatin, and then by diluting with an aqueous medium up to 1% of the solvent while the polymer concentration is maintained at not less than 0.01%. The composition may be stored for long periods without substantial loss of enzymatic activity. The stability of the composition can be improved by incorporating 1 to 8% of salts and, when the composition is likely to be exposed to a bacterial agent of degradation, 0.01 to 0.3% of a bactericidal agent, into the aqueous vehicle for dilution. Thus the stabilised enzyme of the stable composition may be packaged with a substrate and buffer salts. The composition is useful in medical determinations of human and animal functions.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66785676A | 1976-03-17 | 1976-03-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1550753A true GB1550753A (en) | 1979-08-22 |
Family
ID=24679940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1124177A Expired GB1550753A (en) | 1976-03-17 | 1977-03-17 | Stabilized liquid enzyme reagent compositions |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS52134086A (en) |
| CA (1) | CA1091174A (en) |
| CH (1) | CH630662A5 (en) |
| DE (1) | DE2711754C2 (en) |
| FR (1) | FR2344568A1 (en) |
| GB (1) | GB1550753A (en) |
| SE (1) | SE445045B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0054096A1 (en) * | 1980-12-12 | 1982-06-23 | Ivan Endre Modrovich | Method of forming stabilized urease solutions |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1102225A (en) * | 1976-09-13 | 1981-06-02 | Ivan E. Modrovich | Stabilized liquid enzyme and coenzyme compositions and method of preparing same |
| US4132598A (en) * | 1977-06-03 | 1979-01-02 | Modrovich Ivan Endre | Stabilized liquid phosphate containing diagnostic compositions and method of preparing same |
| US4652524A (en) * | 1980-10-02 | 1987-03-24 | Ivan E. Modrovich | Soluble stabilized enzymes |
| DE3370821D1 (en) * | 1982-08-30 | 1987-05-14 | Beckman Instruments Inc | Method for determining _g(g)-glutamyltransferase activity and kits containing a novel substrate solution for use therein |
| JP3203108B2 (en) * | 1993-08-26 | 2001-08-27 | 協和メデックス株式会社 | Method for stabilizing glucose-6-phosphate dehydrogenase |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3557002A (en) * | 1967-11-15 | 1971-01-19 | Procter & Gamble | Stabilized aqueous enzyme preparation |
-
1977
- 1977-03-14 CA CA273,920A patent/CA1091174A/en not_active Expired
- 1977-03-16 FR FR7707873A patent/FR2344568A1/en active Granted
- 1977-03-16 CH CH327077A patent/CH630662A5/en not_active IP Right Cessation
- 1977-03-17 JP JP3101677A patent/JPS52134086A/en active Granted
- 1977-03-17 GB GB1124177A patent/GB1550753A/en not_active Expired
- 1977-03-17 SE SE7703076A patent/SE445045B/en not_active IP Right Cessation
- 1977-03-17 DE DE19772711754 patent/DE2711754C2/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0054096A1 (en) * | 1980-12-12 | 1982-06-23 | Ivan Endre Modrovich | Method of forming stabilized urease solutions |
Also Published As
| Publication number | Publication date |
|---|---|
| CA1091174A (en) | 1980-12-09 |
| SE7703076L (en) | 1977-09-18 |
| DE2711754A1 (en) | 1977-09-22 |
| JPS6117466B2 (en) | 1986-05-07 |
| CH630662A5 (en) | 1982-06-30 |
| SE445045B (en) | 1986-05-26 |
| JPS52134086A (en) | 1977-11-09 |
| FR2344568A1 (en) | 1977-10-14 |
| DE2711754C2 (en) | 1985-09-12 |
| FR2344568B1 (en) | 1980-05-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |