CA2263765C - Procedes de production de particules de proteines utiles dans la diffusion d'agents pharmacologiques - Google Patents
Procedes de production de particules de proteines utiles dans la diffusion d'agents pharmacologiques Download PDFInfo
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- CA2263765C CA2263765C CA2263765A CA2263765A CA2263765C CA 2263765 C CA2263765 C CA 2263765C CA 2263765 A CA2263765 A CA 2263765A CA 2263765 A CA2263765 A CA 2263765A CA 2263765 C CA2263765 C CA 2263765C
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- 238000002372 labelling Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002077 nanosphere Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- INAAIJLSXJJHOZ-UHFFFAOYSA-N pibenzimol Chemical compound C1CN(C)CCN1C1=CC=C(N=C(N2)C=3C=C4NC(=NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 INAAIJLSXJJHOZ-UHFFFAOYSA-N 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011362 radionuclide therapy Methods 0.000 description 1
- 239000012217 radiopharmaceutical Substances 0.000 description 1
- 230000002799 radiopharmaceutical effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000003307 reticuloendothelial effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000009291 secondary effect Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000012134 supernatant fraction Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5169—Proteins, e.g. albumin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/22—Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations
- A61K49/222—Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations characterised by a special physical form, e.g. emulsions, liposomes
- A61K49/223—Microbubbles, hollow microspheres, free gas bubbles, gas microspheres
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1658—Proteins, e.g. albumin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5192—Processes
Landscapes
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Optics & Photonics (AREA)
- Nanotechnology (AREA)
- Acoustics & Sound (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Radiology & Medical Imaging (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Abstract
La présente invention concerne un procédé mis au point en vue de la formation de particules submicromies (nanoparticules) par dénaturation thermique de protéines (telles que l'albumine sérique humaine) en présence d'ions polyvalents (tels que du calcium). Elle concerne également des produits nouveaux élaborés par application dudit procédé. Une concentration appropriée d'ions polyvalents, compris dans une gamme relativement étroite de concentrations, entraîne la précipitation d'une protéine sous la forme de particules colloïdales, à une température bien inférieure à celle de la dénaturation thermique de la protéine (cette température pouvant aller jusqu'à 60 ·C dans le cas de l'albumine sérique). Les températures utilisées dans l'application dudit procédé sont suffisamment basses pour que d'autres molécules puissent être incorporées dans les particules submicromiques de la présente invention, y compris des composés ne supportant pas des températures élevées (cette incorporation se faisant par exemple, par coprécipitation). Ces procédés facilitent la production de nanoparticules et de microparticules contenant diverses molécules (telles que des acides nucléiques, des oligonucléotides, des polynucléotides, de l'ADN, de l'ARN, des polysaccharides, des ribozymes, des composés pharmacologiquement actifs, et d'autres composés similaires) utiles, notamment, à des fins thérapeutiques et diagnostiques. L'adjonction de cations polyvalents sert à la fois à induire la précipitation et à permettre la liaison de molécules à charge négative, telles que l'ADN, avec une protéine à charge négative.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2396896P | 1996-08-19 | 1996-08-19 | |
| US60/023,968 | 1996-08-19 | ||
| PCT/US1997/014661 WO1998007410A1 (fr) | 1996-08-19 | 1997-08-19 | Procedes de production de particules de proteines utiles dans la diffusion d'agents pharmacologiques |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2263765A1 CA2263765A1 (fr) | 1998-02-26 |
| CA2263765C true CA2263765C (fr) | 2010-03-30 |
Family
ID=21818165
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2263765A Expired - Lifetime CA2263765C (fr) | 1996-08-19 | 1997-08-19 | Procedes de production de particules de proteines utiles dans la diffusion d'agents pharmacologiques |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0938299A4 (fr) |
| AU (1) | AU3916997A (fr) |
| CA (1) | CA2263765C (fr) |
| WO (1) | WO1998007410A1 (fr) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6254890B1 (en) | 1997-12-12 | 2001-07-03 | Massachusetts Institute Of Technology | Sub-100nm biodegradable polymer spheres capable of transporting and releasing nucleic acids |
| JP4516273B2 (ja) * | 2000-11-15 | 2010-08-04 | ミナーヴァ・バイオテクノロジーズ・コーポレーション | オリゴヌクレオチド識別子 |
| US8067032B2 (en) | 2000-12-22 | 2011-11-29 | Baxter International Inc. | Method for preparing submicron particles of antineoplastic agents |
| US9700866B2 (en) | 2000-12-22 | 2017-07-11 | Baxter International Inc. | Surfactant systems for delivery of organic compounds |
| EP1392245A2 (fr) * | 2001-04-05 | 2004-03-03 | Universite Laval | Procede de fabrication d'une matrice d'administration et utilisations |
| IL160570A0 (en) | 2001-09-26 | 2004-07-25 | Baxter Int | Preparation of submicron sized nanoparticles via dispersion and solvent or liquid phase removal |
| US20060003012A9 (en) | 2001-09-26 | 2006-01-05 | Sean Brynjelsen | Preparation of submicron solid particle suspensions by sonication of multiphase systems |
| JP4212921B2 (ja) * | 2002-03-29 | 2009-01-21 | 独立行政法人科学技術振興機構 | 抗体を提示するタンパク質中空ナノ粒子を用いる治療薬剤およびタンパク質中空ナノ粒子 |
| IL150906A0 (en) | 2002-07-25 | 2003-02-12 | Yissum Res Dev Co | Diagnostic microspheres |
| SI1585548T1 (sl) | 2002-12-09 | 2018-11-30 | Abraxis Bioscience, Llc | Sestave in metode odmerjanja farmakoloških sredstev |
| FR2902007B1 (fr) * | 2006-06-09 | 2012-01-13 | Flamel Tech Sa | Formulations pharmaceutiques pour la liberation prolongee de principe(s) actif(s) ainsi que leurs applications notamment therapeutiques |
| RU2576609C2 (ru) | 2010-06-04 | 2016-03-10 | АБРАКСИС БАЙОСАЙЕНС, ЭлЭлСи | Способы лечение рака поджелудочной железы |
| EP2510930A1 (fr) | 2011-04-15 | 2012-10-17 | Bionanoplus, S.L. | Nanoparticules comportant des semi-esters de poly (méthyl vinyl éther-co-maléique anhydride) et leurs utilisations |
| MX2017005692A (es) | 2014-10-31 | 2017-08-07 | Glaxosmithkline Ip Dev Ltd | Formulacion en polvo. |
| CN110302364A (zh) * | 2019-06-24 | 2019-10-08 | 浙江工商大学 | 一种自组装过氧化氢酶纳米颗粒及其制备方法和应用 |
| CN115154651B (zh) * | 2022-06-23 | 2023-10-27 | 华中科技大学 | 一种生物矿化的牛血清白蛋白@钙硒纳米球、制备方法及应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6020059B2 (ja) * | 1980-09-16 | 1985-05-20 | 雪印乳業株式会社 | 食品または医薬品に適したマイクロカプセルの製造法 |
| US5585112A (en) * | 1989-12-22 | 1996-12-17 | Imarx Pharmaceutical Corp. | Method of preparing gas and gaseous precursor-filled microspheres |
| GB9106686D0 (en) * | 1991-03-28 | 1991-05-15 | Hafslund Nycomed As | Improvements in or relating to contrast agents |
| JP3482242B2 (ja) * | 1994-06-10 | 2003-12-22 | 株式会社キティー | 生理活性物質の封入されている微粒子及びその製法 |
-
1997
- 1997-08-19 AU AU39169/97A patent/AU3916997A/en not_active Abandoned
- 1997-08-19 CA CA2263765A patent/CA2263765C/fr not_active Expired - Lifetime
- 1997-08-19 WO PCT/US1997/014661 patent/WO1998007410A1/fr not_active Application Discontinuation
- 1997-08-19 EP EP97936517A patent/EP0938299A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998007410A1 (fr) | 1998-02-26 |
| EP0938299A1 (fr) | 1999-09-01 |
| EP0938299A4 (fr) | 2001-01-17 |
| CA2263765A1 (fr) | 1998-02-26 |
| AU3916997A (en) | 1998-03-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |