CA2255952A1 - Capteur d'acide nucleique - Google Patents

Capteur d'acide nucleique Download PDF

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Publication number
CA2255952A1
CA2255952A1 CA002255952A CA2255952A CA2255952A1 CA 2255952 A1 CA2255952 A1 CA 2255952A1 CA 002255952 A CA002255952 A CA 002255952A CA 2255952 A CA2255952 A CA 2255952A CA 2255952 A1 CA2255952 A1 CA 2255952A1
Authority
CA
Canada
Prior art keywords
membrane
nucleic acid
spanning
biosensor
proximal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002255952A
Other languages
English (en)
Inventor
Margaret Harding
Sally Wright Lucas
Vijoleta Lucija Bronislava Braach-Maksvytis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ambri Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2255952A1 publication Critical patent/CA2255952A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

La présente invention se rapporte à des biocapteurs et à des procédés de détection de séquences d'acides nucléiques sélectionnés. Dans une des réalisations de l'invention, ce biocapteur comporte une électrode et une membrane bicouche. Ladite membrane comporte une couche inférieure et une couche supérieure. La couche inférieure se trouve à proximité de l'électrode, à laquelle elle est couplée de telle manière à qu'il existe un espace entre la membrane et l'électrode, la conductance ou l'impédance de la membrane étant fonction de la présence ou de l'absence de la séquence de l'acide nucléique sélectionné. Ladite membrane comporte un réseau serré de molécules amphiphiles et une pluralité de canaux ioniques comportant des monomères recouvrant une première moitié de la membrane, dispersés au sein de la couche supérieure, et des monomères recouvrant une seconde moitié de la membrane, dispersés au sein de la couche inférieure. Les monomères recouvrant la première moitié de la membrane sont aptes à une diffusion latérale au sein de la couche supérieure et les monomères recouvrant la seconde moitié de la membrane sont bloqués vis à vis d'une diffusion latérale au sein de la couche inférieure. Un premier ligand, susceptible de réagir spécifiquement avec la séquence de l'acide nucléique sélectionné, est fixé à une extrémité d'une fraction des monomères recouvrant la première moitié de la membrane, à proximité de la surface de la membrane et un second ligand, susceptible de réagir spécifiquement avec l'acide nucléique sélectionné ou avec un marqueur fixé à cet acide, est fixé à une extrémité du reste des monomères recouvrant la première moitié de la membrane, à proximité de la surface de la membrane.
CA002255952A 1996-05-22 1997-05-22 Capteur d'acide nucleique Abandoned CA2255952A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1812596P 1996-05-22 1996-05-22
US60/018,125 1996-05-22
PCT/AU1997/000316 WO1997044651A1 (fr) 1996-05-22 1997-05-22 Capteur d'acide nucleique

Publications (1)

Publication Number Publication Date
CA2255952A1 true CA2255952A1 (fr) 1997-11-27

Family

ID=21786371

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002255952A Abandoned CA2255952A1 (fr) 1996-05-22 1997-05-22 Capteur d'acide nucleique

Country Status (5)

Country Link
EP (1) EP0990149A4 (fr)
JP (1) JP2000513811A (fr)
AU (1) AU734086B2 (fr)
CA (1) CA2255952A1 (fr)
WO (1) WO1997044651A1 (fr)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) * 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US5952172A (en) 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
AUPN366895A0 (en) * 1995-06-20 1995-07-13 Australian Membrane And Biotechnology Research Institute Detection of small analytes
US6387625B1 (en) 1995-06-27 2002-05-14 The University Of North Carolina At Chapel Hill Monolayer and electrode for detecting a label-bearing target and method of use thereof
US6127127A (en) * 1995-06-27 2000-10-03 The University Of North Carolina At Chapel Hill Monolayer and electrode for detecting a label-bearing target and method of use thereof
US6444423B1 (en) 1996-06-07 2002-09-03 Molecular Dynamics, Inc. Nucleosides comprising polydentate ligands
US7393645B2 (en) 1996-11-05 2008-07-01 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US6096273A (en) * 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7045285B1 (en) 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
US7014992B1 (en) * 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US7160678B1 (en) 1996-11-05 2007-01-09 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US6503452B1 (en) * 1996-11-29 2003-01-07 The Board Of Trustees Of The Leland Stanford Junior University Biosensor arrays and methods
US6306584B1 (en) * 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
CA2293744A1 (fr) 1997-06-12 1998-12-17 Clinical Micro Sensors, Inc. Procedes electroniques de detection d'analytes
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
IL121312A (en) 1997-07-14 2001-09-13 Technion Res & Dev Foundation Microelectronic components, their manufacture and electronic networks containing them
US6686150B1 (en) 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6761816B1 (en) 1998-06-23 2004-07-13 Clinical Micro Systems, Inc. Printed circuit boards with monolayers and capture ligands
US6740518B1 (en) 1998-09-17 2004-05-25 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
US6541617B1 (en) 1998-10-27 2003-04-01 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
IL126776A (en) 1998-10-27 2001-04-30 Technion Res & Dev Foundation A method of investing gold
US6833267B1 (en) 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US7364920B2 (en) 1999-10-27 2008-04-29 Technion Research And Development Foundation Ltd. Method for gold deposition
DE10015547C2 (de) * 2000-03-30 2002-02-14 Aventis Res & Tech Gmbh & Co Verfahren zur Detektion von Molekülen mittels Impedanzspektroskopie und Vorrichtung zur Durchführung dieser Verfahren
JP2003533211A (ja) * 2000-05-18 2003-11-11 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ 脂質二重層アレイ方法およびデバイス
WO2005016115A2 (fr) 2003-01-23 2005-02-24 Montana State University Biocapteurs faisant intervenir des ligands immobilises sur des dendrimeres et utilisation de ceux-ci
US7824927B2 (en) * 2005-04-05 2010-11-02 George Mason Intellectual Properties, Inc. Analyte detection using an active assay
US8906609B1 (en) 2005-09-26 2014-12-09 Arrowhead Center, Inc. Label-free biomolecule sensor based on surface charge modulated ionic conductance
JP2018533010A (ja) * 2015-10-21 2018-11-08 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ナノポアベースのｄｎａ配列決定のための脂質二重層形成を支援するための撥水性層としてのフルオロポリマーの使用
CN113070113B (zh) * 2021-06-03 2021-08-20 成都齐碳科技有限公司 芯片结构、成膜方法、纳米孔测序装置及应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0770874A3 (fr) * 1989-01-27 1998-01-21 Australian Membrane And Biotechnology Research Institute Membranes réceptrices et sélection par ionophores
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch
AU666113B2 (en) * 1991-03-27 1996-02-01 Ambri Limited Ionic reservoir at electrode surface
PT695425E (pt) * 1993-04-21 2003-02-28 Ambri Ltd Amplificador de superficie
AUPM950094A0 (en) * 1994-11-16 1994-12-08 Australian Membrane And Biotechnology Research Institute Detection device and method
AUPN366995A0 (en) * 1995-06-20 1995-07-13 Australian Membrane And Biotechnology Research Institute Self-assembly of bilayer membrane sensors

Also Published As

Publication number Publication date
EP0990149A1 (fr) 2000-04-05
EP0990149A4 (fr) 2003-02-26
AU734086B2 (en) 2001-05-31
JP2000513811A (ja) 2000-10-17
WO1997044651A1 (fr) 1997-11-27
AU2757897A (en) 1997-12-09

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