CA2255057A1 - Expression de genes dans les plantes - Google Patents
Expression de genes dans les plantes Download PDFInfo
- Publication number
- CA2255057A1 CA2255057A1 CA002255057A CA2255057A CA2255057A1 CA 2255057 A1 CA2255057 A1 CA 2255057A1 CA 002255057 A CA002255057 A CA 002255057A CA 2255057 A CA2255057 A CA 2255057A CA 2255057 A1 CA2255057 A1 CA 2255057A1
- Authority
- CA
- Canada
- Prior art keywords
- rna
- sequence
- seq
- nucleotide
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000014509 gene expression Effects 0.000 title claims description 42
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims abstract description 316
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 238
- 238000013519 translation Methods 0.000 claims abstract description 237
- 230000002708 enhancing effect Effects 0.000 claims abstract description 131
- 108091026890 Coding region Proteins 0.000 claims abstract description 104
- 108020004414 DNA Proteins 0.000 claims abstract description 96
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 94
- 210000004027 cell Anatomy 0.000 claims abstract description 84
- 108020003589 5' Untranslated Regions Proteins 0.000 claims abstract description 41
- 108020005345 3' Untranslated Regions Proteins 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 32
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims abstract description 26
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims abstract description 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 23
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 22
- 108091027544 Subgenomic mRNA Proteins 0.000 claims abstract description 21
- 241000700605 Viruses Species 0.000 claims abstract description 21
- 108091092236 Chimeric RNA Proteins 0.000 claims abstract description 18
- 229920001184 polypeptide Polymers 0.000 claims abstract description 18
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims abstract description 15
- 108010009460 RNA Polymerase II Proteins 0.000 claims abstract description 10
- 102000009572 RNA Polymerase II Human genes 0.000 claims abstract description 10
- 230000008569 process Effects 0.000 claims abstract description 10
- 230000001419 dependent effect Effects 0.000 claims abstract description 8
- 108091093105 Nuclear DNA Proteins 0.000 claims abstract description 7
- 230000001131 transforming effect Effects 0.000 claims abstract description 7
- 210000000805 cytoplasm Anatomy 0.000 claims abstract description 5
- 230000001172 regenerating effect Effects 0.000 claims abstract description 5
- 241000894007 species Species 0.000 claims abstract description 5
- 239000002773 nucleotide Substances 0.000 claims description 372
- 125000003729 nucleotide group Chemical group 0.000 claims description 372
- 241000196324 Embryophyta Species 0.000 claims description 163
- 239000012634 fragment Substances 0.000 claims description 121
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 43
- 241000709653 Tobacco necrosis virus A Species 0.000 claims description 30
- 244000061176 Nicotiana tabacum Species 0.000 claims description 28
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 28
- 108020004705 Codon Proteins 0.000 claims description 24
- 101710137500 T7 RNA polymerase Proteins 0.000 claims description 21
- 240000008042 Zea mays Species 0.000 claims description 16
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 16
- 108091081024 Start codon Proteins 0.000 claims description 14
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims description 14
- 241001515965 unidentified phage Species 0.000 claims description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 12
- 235000005822 corn Nutrition 0.000 claims description 12
- 235000021307 Triticum Nutrition 0.000 claims description 11
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims description 9
- 240000007594 Oryza sativa Species 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 230000000749 insecticidal effect Effects 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 241001057636 Dracaena deremensis Species 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 240000002791 Brassica napus Species 0.000 claims description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 3
- 240000003768 Solanum lycopersicum Species 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 101150102059 cry3Aa gene Proteins 0.000 claims description 3
- 241000219198 Brassica Species 0.000 claims description 2
- 229920000742 Cotton Polymers 0.000 claims description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 2
- 108010013845 RNA Polymerase I Proteins 0.000 claims description 2
- 102000017143 RNA Polymerase I Human genes 0.000 claims description 2
- 108010078067 RNA Polymerase III Proteins 0.000 claims description 2
- 102000014450 RNA Polymerase III Human genes 0.000 claims description 2
- -1 cry3C Proteins 0.000 claims description 2
- 101100497233 Bacillus thuringiensis subsp. aizawai cry1Da gene Proteins 0.000 claims 1
- 101100497235 Bacillus thuringiensis subsp. kenyae cry1Ea gene Proteins 0.000 claims 1
- 244000299507 Gossypium hirsutum Species 0.000 claims 1
- 240000005979 Hordeum vulgare Species 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 101150108868 cry1Ba gene Proteins 0.000 claims 1
- 238000009331 sowing Methods 0.000 claims 1
- 238000013518 transcription Methods 0.000 abstract description 20
- 230000035897 transcription Effects 0.000 abstract description 20
- 101150039863 Rich gene Proteins 0.000 abstract description 2
- 239000013612 plasmid Substances 0.000 description 108
- 150000007523 nucleic acids Chemical group 0.000 description 74
- 235000018102 proteins Nutrition 0.000 description 74
- 102000039446 nucleic acids Human genes 0.000 description 72
- 108020004707 nucleic acids Proteins 0.000 description 72
- 241000709655 unidentified tobacco necrosis virus Species 0.000 description 59
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 56
- 238000000338 in vitro Methods 0.000 description 43
- 210000001938 protoplast Anatomy 0.000 description 42
- 108091034117 Oligonucleotide Proteins 0.000 description 41
- 241000726448 Satellite tobacco mosaic virus Species 0.000 description 32
- 229920001940 conductive polymer Polymers 0.000 description 30
- 238000009616 inductively coupled plasma Methods 0.000 description 30
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 29
- 108020004999 messenger RNA Proteins 0.000 description 28
- 241000282326 Felis catus Species 0.000 description 25
- 230000009466 transformation Effects 0.000 description 24
- 101710132601 Capsid protein Proteins 0.000 description 21
- 101710094648 Coat protein Proteins 0.000 description 21
- 101710125418 Major capsid protein Proteins 0.000 description 21
- 101710141454 Nucleoprotein Proteins 0.000 description 21
- 101710083689 Probable capsid protein Proteins 0.000 description 21
- 239000002299 complementary DNA Substances 0.000 description 20
- 239000013598 vector Substances 0.000 description 20
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 19
- 238000004520 electroporation Methods 0.000 description 17
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 15
- 238000003752 polymerase chain reaction Methods 0.000 description 15
- 108700026244 Open Reading Frames Proteins 0.000 description 14
- 238000009825 accumulation Methods 0.000 description 14
- 238000001727 in vivo Methods 0.000 description 14
- 239000003550 marker Substances 0.000 description 14
- 108020000999 Viral RNA Proteins 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 12
- 239000000047 product Substances 0.000 description 11
- 230000014621 translational initiation Effects 0.000 description 11
- 241000209140 Triticum Species 0.000 description 10
- 239000002253 acid Substances 0.000 description 10
- 101150103518 bar gene Proteins 0.000 description 10
- 238000010367 cloning Methods 0.000 description 10
- 230000037430 deletion Effects 0.000 description 10
- 238000012217 deletion Methods 0.000 description 10
- 230000004927 fusion Effects 0.000 description 10
- 238000003780 insertion Methods 0.000 description 10
- 230000037431 insertion Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 101100497223 Bacillus thuringiensis cry1Ag gene Proteins 0.000 description 8
- 108091036407 Polyadenylation Proteins 0.000 description 8
- 108020004511 Recombinant DNA Proteins 0.000 description 8
- 241001493065 dsRNA viruses Species 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000001105 regulatory effect Effects 0.000 description 8
- 238000011144 upstream manufacturing Methods 0.000 description 8
- 150000007513 acids Chemical class 0.000 description 7
- 238000010276 construction Methods 0.000 description 7
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 7
- 230000000638 stimulation Effects 0.000 description 7
- 102000053602 DNA Human genes 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 230000004936 stimulating effect Effects 0.000 description 6
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 6
- 231100000331 toxic Toxicity 0.000 description 6
- 230000002588 toxic effect Effects 0.000 description 6
- 230000009261 transgenic effect Effects 0.000 description 6
- 241000701489 Cauliflower mosaic virus Species 0.000 description 5
- 238000012286 ELISA Assay Methods 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 5
- 241000723873 Tobacco mosaic virus Species 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 230000003612 virological effect Effects 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 108091092195 Intron Proteins 0.000 description 4
- 108010064851 Plant Proteins Proteins 0.000 description 4
- 108091023045 Untranslated Region Proteins 0.000 description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 210000001072 colon Anatomy 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 4
- 235000021118 plant-derived protein Nutrition 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 108091035707 Consensus sequence Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 101710128836 Large T antigen Proteins 0.000 description 3
- 240000007377 Petunia x hybrida Species 0.000 description 3
- 108091034057 RNA (poly(A)) Proteins 0.000 description 3
- 101150084101 RNA2 gene Proteins 0.000 description 3
- 101100353432 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRP2 gene Proteins 0.000 description 3
- 102000039471 Small Nuclear RNA Human genes 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 101150055766 cat gene Proteins 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000000977 initiatory effect Effects 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000002706 plastid Anatomy 0.000 description 3
- 230000008488 polyadenylation Effects 0.000 description 3
- 230000001124 posttranscriptional effect Effects 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 108091029842 small nuclear ribonucleic acid Proteins 0.000 description 3
- 229940113082 thymine Drugs 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 241000589158 Agrobacterium Species 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108090000565 Capsid Proteins Proteins 0.000 description 2
- 108700010070 Codon Usage Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241000701832 Enterobacteria phage T3 Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- 108091023242 Internal transcribed spacer Proteins 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 241001147398 Ostrinia nubilalis Species 0.000 description 2
- 101150102573 PCR1 gene Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 108020005089 Plant RNA Proteins 0.000 description 2
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 2
- 241001515849 Satellite Viruses Species 0.000 description 2
- 108020004566 Transfer RNA Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 108091026822 U6 spliceosomal RNA Proteins 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 238000005567 liquid scintillation counting Methods 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 238000000329 molecular dynamics simulation Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000007857 nested PCR Methods 0.000 description 2
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108700022487 rRNA Genes Proteins 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 229940035893 uracil Drugs 0.000 description 2
- 108020004463 18S ribosomal RNA Proteins 0.000 description 1
- 101710088758 23kDa protein Proteins 0.000 description 1
- SOFPIAMTOZWXKT-UHFFFAOYSA-N 2h-1,2,4-triazine-3-thione Chemical compound SC1=NC=CN=N1 SOFPIAMTOZWXKT-UHFFFAOYSA-N 0.000 description 1
- JCLFHZLOKITRCE-UHFFFAOYSA-N 4-pentoxyphenol Chemical compound CCCCCOC1=CC=C(O)C=C1 JCLFHZLOKITRCE-UHFFFAOYSA-N 0.000 description 1
- 101150008391 A1 gene Proteins 0.000 description 1
- 241000218473 Agrotis Species 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108010087765 Antipain Proteins 0.000 description 1
- 238000006677 Appel reaction Methods 0.000 description 1
- 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 108091032955 Bacterial small RNA Proteins 0.000 description 1
- 241000709756 Barley yellow dwarf virus Species 0.000 description 1
- 101710183938 Barstar Proteins 0.000 description 1
- 241000714207 Carmovirus Species 0.000 description 1
- 108050001186 Chaperonin Cpn60 Proteins 0.000 description 1
- 102000052603 Chaperonins Human genes 0.000 description 1
- 241000219312 Chenopodium Species 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 241000588923 Citrobacter Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 102100029376 Cryptochrome-1 Human genes 0.000 description 1
- 101710151559 Crystal protein Proteins 0.000 description 1
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 1
- 101100109110 Danio rerio aph1b gene Proteins 0.000 description 1
- 241000489975 Diabrotica Species 0.000 description 1
- 240000006497 Dianthus caryophyllus Species 0.000 description 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 241000710188 Encephalomyocarditis virus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 101000919351 Homo sapiens Cryptochrome-1 Proteins 0.000 description 1
- 241000209219 Hordeum Species 0.000 description 1
- 244000075920 Hordeum bulbosum Species 0.000 description 1
- 235000007338 Hordeum bulbosum Nutrition 0.000 description 1
- 108091029795 Intergenic region Proteins 0.000 description 1
- 108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 1
- 241000724186 Klebsiella phage No. 11 Species 0.000 description 1
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
- 241000359194 Lisianthus necrosis virus Species 0.000 description 1
- 241000714212 Melon necrotic spot virus Species 0.000 description 1
- 101100247628 Mus musculus Rcl1 gene Proteins 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 101100293593 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) nar-1 gene Proteins 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- XDMCWZFLLGVIID-SXPRBRBTSA-N O-(3-O-D-galactosyl-N-acetyl-beta-D-galactosaminyl)-L-serine Chemical compound CC(=O)N[C@H]1[C@H](OC[C@H]([NH3+])C([O-])=O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 XDMCWZFLLGVIID-SXPRBRBTSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 1
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241001499750 Pseudomonad phage gh-1 Species 0.000 description 1
- 238000013381 RNA quantification Methods 0.000 description 1
- 230000021839 RNA stabilization Effects 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241001468001 Salmonella virus SP6 Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000187391 Streptomyces hygroscopicus Species 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 241000709669 Tobacco necrosis virus D Species 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical class N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- SDNYTAYICBFYFH-TUFLPTIASA-N antipain Chemical compound NC(N)=NCCC[C@@H](C=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 SDNYTAYICBFYFH-TUFLPTIASA-N 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- PXXJHWLDUBFPOL-UHFFFAOYSA-N benzamidine Chemical compound NC(=N)C1=CC=CC=C1 PXXJHWLDUBFPOL-UHFFFAOYSA-N 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 101150065438 cry1Ab gene Proteins 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000000408 embryogenic effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
- 108010052968 leupeptin Proteins 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000007620 mathematical function Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 235000020030 perry Nutrition 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 102000028499 poly(A) binding Human genes 0.000 description 1
- 108091023021 poly(A) binding Proteins 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 108091007054 readthrough proteins Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 210000004708 ribosome subunit Anatomy 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 102200111112 rs397514590 Human genes 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003118 sandwich ELISA Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000010421 standard material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 230000005029 transcription elongation Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 150000003918 triazines Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La présente invention concerne des gènes chimères. Ces gènes comprennent un premier promoteur reconnu par une polymérase d'ARN dépendante de l'ADN, différente d'une polymérase II d'ARN eucaryote. Une zone d'ADN codant un ARN chimère qui comprend un UTR 5' une séquence de codage hétérologue riche en AU, un UTR 3', et éventuellement une séquence de terminaison reconnue par cette polymérase d'ARN. Le premier promoteur et la zone d'ADN codant l'ARN chimère sont liés de manière opérationnelle de telle sorte que lors de la transcription par la polymérase d'ARN, une espèce d'ARN sans addition de chapeau est produite qui comprend une première séquence de stimulation de traduction dérivée de la zone 5' de l'ARN génomique ou sous-génomique d'un virus de plante à ARN à brins positifs; une séquence de codage d'ARN hétérologue codant un polypeptide ou une protéine interressante, de préférence, à partir d'un gène riche en AT et enfin une deuxième séquence de stimulation de la traduction dérivée de la zone 3' de l'ARN génomique ou sous-génomique d'un virus de plant à ARN à brins positifs. Cette espèce d'ARN sans addition de chapeau peut être traduite dans le cytoplasme d'une cellule de plante pour produire la protéine ou le polypeptide. L'invention concerne également des cellules de plantes et des plantes comprenant ces gènes chimères, intégrés dans leur ADN nucléaire, ces cellules produisant ainsi les polymérases d'ARN correspondant aux promoteurs et aux terminateurs utilisés. En outre, l'invention a pour objet un procédé pour produire une plante exprimant une protéine ou un polypeptide codé par un gène hétérologue qui comprend les étapes consistant à transformer le génome nucléaire d'une cellule de plante avec les gènes chimères susmentionnés, et regénérer une plante transformée à partir de la cellule transformée.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66773196A | 1996-06-21 | 1996-06-21 | |
| US08/667,731 | 1996-06-21 | ||
| PCT/EP1997/002832 WO1997049814A1 (fr) | 1996-06-21 | 1997-05-30 | Expression de genes dans les plantes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2255057A1 true CA2255057A1 (fr) | 1997-12-31 |
Family
ID=24679404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002255057A Abandoned CA2255057A1 (fr) | 1996-06-21 | 1997-05-30 | Expression de genes dans les plantes |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0922104A1 (fr) |
| JP (1) | JP2000513217A (fr) |
| AU (1) | AU725002B2 (fr) |
| CA (1) | CA2255057A1 (fr) |
| WO (1) | WO1997049814A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
| KR20010042069A (ko) | 1998-03-20 | 2001-05-25 | 베니텍 오스트레일리아 리미티드 | 유전자 발현 조절방법 |
| JP2002537842A (ja) * | 1999-03-09 | 2002-11-12 | ラージ スケール バイオロジー コーポレイション | 外来の配列を発現するための多成分ｒｎａベクター系 |
| US7148400B1 (en) | 1999-04-20 | 2006-12-12 | Bayer Bioscience N.V. | Methods and means for delivering inhibitory RNA to plants and applications thereof |
| US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
| AUPR621501A0 (en) | 2001-07-06 | 2001-08-02 | Commonwealth Scientific And Industrial Research Organisation | Delivery of ds rna |
| IN2014CN00793A (fr) * | 2011-08-01 | 2015-04-03 | Basf Plant Science Co Gmbh |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5135855A (en) * | 1986-09-03 | 1992-08-04 | The United States Of America As Represented By The Department Of Health And Human Services | Rapid, versatile and simple system for expressing genes in eukaryotic cells |
| US5409823A (en) * | 1992-09-24 | 1995-04-25 | Ciba-Geigy Corporation | Methods for the production of hybrid seed |
-
1997
- 1997-05-30 WO PCT/EP1997/002832 patent/WO1997049814A1/fr not_active Application Discontinuation
- 1997-05-30 AU AU31704/97A patent/AU725002B2/en not_active Ceased
- 1997-05-30 CA CA002255057A patent/CA2255057A1/fr not_active Abandoned
- 1997-05-30 JP JP10502180A patent/JP2000513217A/ja active Pending
- 1997-05-30 EP EP97927090A patent/EP0922104A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP0922104A1 (fr) | 1999-06-16 |
| WO1997049814A1 (fr) | 1997-12-31 |
| AU3170497A (en) | 1998-01-14 |
| AU725002B2 (en) | 2000-10-05 |
| JP2000513217A (ja) | 2000-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5994526A (en) | Gene expression in plants | |
| CA2108000C (fr) | Expression genique amelioree chez les plantes en utilisant la sequence hsp70 intron | |
| Goderis et al. | A set of modular plant transformation vectors allowing flexible insertion of up to six expression units | |
| AU648951B2 (en) | Agrobacterium mediated transformation of germinating plant seeds | |
| US5589616A (en) | Monocot seed storage proteins in dicots | |
| WO1993001283A1 (fr) | Plantes transgeniques sans genes de selection | |
| US8003777B2 (en) | Use of untranslated region of osmotin gene to enhance transgene expression in plants | |
| EP3320097B1 (fr) | Procédé d'optimisation de gène | |
| EP0840793B1 (fr) | Sequence de regulation d'expression utilisee dans l'expression generale et effective de genes dans les vegetaux | |
| AU725002B2 (en) | Gene expression in plants | |
| WO2001018192A2 (fr) | Vecteurs synthetiques propres, plasmides, vegetaux transgeniques et fractions de vegetaux les renfermant, et methodes d'obtention | |
| Cerny et al. | RNA-binding protein-mediated translational repression of transgene expression in plants | |
| CA2244959A1 (fr) | Expression genetique dans les plantes | |
| CA2341278A1 (fr) | Sequences d'adn synthetiques modifiees pour une meilleure action insecticide | |
| AU705682C (en) | An expression control sequence for general and effective expression of genes in plants | |
| AU2004200330B2 (en) | Use of untranslated region of osmotin gene to enhance transgene expression in plants | |
| CA2198723C (fr) | Regulateurs de transcription vegetale issus de circovirus | |
| Barry | Isolation of a sugarcane gene promoter and its application to chimeric gene expression in sugarcane protoplasts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |