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University of Alberta
McGill University
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Individual
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Priority claimed from PCT/CA1997/000275external-prioritypatent/WO1997041424A1/en
Publication of CA2252474A1publicationCriticalpatent/CA2252474A1/en
Application grantedgrantedCritical
Publication of CA2252474CpublicationCriticalpatent/CA2252474C/en
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an .alpha.-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
CA002252474A1996-04-251997-04-25Biosensor device and method
Expired - Fee RelatedCA2252474C
(en)
Test carrier for the analysis of a sample liquid, process for the carrying out of such an analysis and process for producing a fixing layer for a test carrier
One-way reaction vessel for the solid-phase immunological analysis of, and a method of measuring constituents which can be determined via immune reactions
Polypeptides having human dopaminergic receptor activity, nucleic acids coding for said polypeptides and uses thereof for screening active substances on said polypeptides