CA2252110C - 5-androstene 3.beta.,17.alpha. diol as an inhibitor of tumor growth - Google Patents

Abstract

The instant invention provides means of accelerating cell aging and programm ed cell death in tumor cells by administration of 3.beta.,17.alpha. androstenediol (.alpha.AED) or its ethers or esters.

Description

Title: 5-ANDROSTENE 3(3, 17a D1OL AS A7V f1'OR OP TUMOR
GRO'~
l3aek~rotmd of the invention Field of the invention:
This invention relates to the field of ph~.m,sceuticals for himor-inhibitory effects. The 5-androstene 3~3, 17a diol (aAED), it;; esters and ethers, are taught herein to achieve tumor-inhibiting effect.
Description of Related Art:
Mifepristone (RU486) is used as a pmgestE:rone receptor antagonist (See U.S. Patent 4,386,0$5) arid has been shown to have nse both as an abortifacient and has been found useful for treating steroid-dependent breast cancer.
Flutamide, which has been disclosed in U.S. Patent 3,847,988 is an antiandrogen that has been used to treat prostatic cancer, usually in conjunction with estrogen.
U.S. Patent 2,52 X,586 to Levy, et al., teaches production of the 17-monobenzoate ester of androstene diol. No use of the 5-androstene 3(3, 17a diol (aAEfa) is taught therein.
Peat, in U.S. Patent 4,628,052 teaches a l;enus which might, arguably, within the scope of the genus, encompass the aAED_ However; all examples and all named compounds require a keto group. Hence, it is reasonable to conclude that the aAP,D is not intended therein.
Tir~dall, in U.S. Fatent 2,845,381 teaches cosmetic compositions containing the aAED. No medicinal compositions approp~zaie for internal use ox medicinal uses are suggested therein.
U_S. Patent 4,882,322 to Johnson, et a1. teaches substituted 5-androstene 3(3, 17 (3 diol to regulate or inhibit the conversion of androgens to estogens. The aAED is not taught therein.
Swartz, et al., in U.S. Patent 4,898,694 teaches a very large group of compounds which encompass substituted androstenediols. However, Schwartz does not suggest the aAED nor the esters and ethers claimed herein for any purpose.
Loria, in U.S. Patents 5,206,008, 5,277,907, 5,387,583, 5,461,042 and 5,478,566 teaches that the 5-androstene 3(3, 17~ diol ([3AED) and 5, -androstene 3(3, 7~, 17(3 triol (AET) enhance immune response, and also are useful for counteracting the untoward effects of irradiation and chemotherapy, and buffer the anti-proliferative effects of hydrocortisone. None of these patents teaches or suggests use of aAED. As taught therein, the (3AED is most effective if administered in such a manner that it contacts tissue of ectodermal origin.
Summary of the Invention:
The instant invention provides a means of accelerating cell aging and programmed cell death in tumor cells. The practice of the invention involves administration of 3(3, 17a androstenetriol (which may be referred to in this application as either l7aAED or simply aAED) and esters and ethers thereof.
Advantageously, according to a first preferred embodiment, the present invention relates to a composition of matter comprising a tumor proliferation inhibiting effective amount of aAED or an ester or ether thereof of the formula:
OR
RC

2a wherein R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R~ is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, in a form appropriate for oral administration. More preferably, the composition may be a tablet, a capsule or a liquid.
Advantageously, according to a second preferred embodiment, the present invention relates to a sterile composition of matter comprising a tumor proliferation inhibiting effective amount of aAED or an ester or ether thereof of the formula:
OR
wherein R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, in a 2b sterile solution appropriate for parenteral administration. More preferably, said sterile composition may be in single dosage form in a container, in an isotonic solution or containing the aAED in a cyclodextrin inclusion complex.
Advantageously, according to a third preferred embodiment, the present invention relates to a a tumor proliferation inhibiting effective amount of a compound of the formula:
OR
RO
wherein either R may be H, alkenyl of 2-8 carbons, all~yl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein RZ is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, on a solid support. More preferably, said composition is a patch for application to the skin or is a sponge.
Advantageously, according to a fourth preferred embodiment, the present invention relates to a tumor proliferation inhibiting effective amount of a compound of the formula:

OR
RO
wherein either R may be H, alkenyl of 2-8 carbons, allcyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein RZ is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, in a pharmaceutically acceptable carrier wherein said composition contains, additionally, as an active agent, a second anti-cancer drug.
More preferably, the second anti-cancer drug may be:
~ a antiandrogen or antiestrogen, ~ a urea;
~ an alkylating agent;
~ an antimetabolite;
~ a DNA intercalating agent;
~ an interferon;
~ a nucleic acid inhibitor;
~ a vinca alkaloid; or 2d ~ RU486.
Advantageously, according to~ a fifth preferred embodiment, the present invention relates to a use of a coz~tpound of formula:
oR
wherein either R may be H, alkenyl of 2-$ carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein RZ is H; allcyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the al><yl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, ox tk~e all~yl rnay be wholly or partially eyclized, for the manufacture of a medicament for the acceleration of the aging of cells and programmatian of the death of cells.
Advantageously, according to a sixth preferred embodiment, the present invention relates to a use of a coxnpound of .formula_ OR

2e wherein either R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, and its esters and ethers, in the acceleration of the aging of cells and programmation of the death of cells.
Description of aarticularlv preferred embodiments of the Invention:
The instant invention relates to the use of 5-androstene 3(3, 17a diol (herein referred tp as aAED or l7aAED), its esters and ethers, to inhibit growth and accelerate cell aging, induce apoptosis and death of tumor cells as a means of treating malignancies. The active agents of the invention may also be used as contraceptives and abortifacients. The active agents are of the structure:

OR
wherein R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or CORZ, wherein RZ
is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl.
Any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-9 carbons, halo, alkoxy of I-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized.
It has been found that these active agents, when administered as described herein, inhibit cell growth. The aAED is administered in sufficient dosages to provide a blood concentration of from 5 to 10,000 nM when given systemically.
A more preferred blood or tissue fluid concentration is in the rang of 10 to 10,000 nM. The dosage will vary with the type of cell to be inhibited. The method of administration will depend on the location of the target cells. Such means as parenteral or oral administration are also appropriate. The aAED may also be administered by applicator or in a spray to tissue during surgery. Compositions containing the. active agents taught herein may be administered vaginally or rectally either by instillation of a liquid composition or on supports such as sponges.
Other preferred methods of administration include buccal, sublingual, nasal or endotracheal routes. Sprays or mists may be useful for such administration. Furthermore, sprays may be useful for administration to the operative area during surgery.

For example, sprays may be used to contact the peritoneal cavity or the thoracic cavity during surgery.
Compositions of the invention may also be administered to the intestinal mucosa by oral or rectal routes. Suppositories, solutions for use as retention enemas and creams or jellies are appropriate means for use in rectal administration.
Compositions of the invention may also be applied to the vaginal mucosa using creams, jellies suppositories or douching solutions. The compositions may be in the form of prophylactic vaginal preparations or may be used in lubricants on condoms.
Jellies and creams may also be administered by application in a cervical diaphragm which, when in place, will provide for prolonged contact with the cervix.
For purposes of administration into an orifice of the body, the compositions of the invention may be administered via a flexible tube into the target site. As indicated previously, the compositions containing aAED may be administered as a douche or retention enema. Other target sites include the bladder, uterus, trachea, nasopharynx, sinus or (via the nasal passage) the pituitary.
The carrier system used in a given instance will depend on the mode of administration. The active agents are lipophilic compounds. Solvents and carriers for lipophilic steroids known in the art are appropriate for use in the compositions containing aAED or the esters and ethers of aAED.
Examples of such carriers are glycols such as polypropylene glycol, polyethylene glycol, ethanol, DMSO and cyclodextrins (especially the amorphous cyclodextrins). Cyclodextrins will pass through the buccal mucosa into the circulation easily.
This method is particularly appropriate for administration as a means of avoiding intravenous administration while bypassing the liver. Other vehicles that should be considered include fatty acid esters of polyoxyethylene sorbital (Tweens) or sorbitan (spans) for preparation of emulsions.

The compositions taught herein may be used to treat most neoplasms, including for, far example, use in treatment of neoplasms, including those of the blood-forming organs, the liver, pancreas, thyroid, andrenals, pituitary, ovaries, 5 testicles, breast, central nervous system (including brain, spinal column ). bone, connective tissue, lungs, liver, the gastro-intestinal system, connective tissue, uterus, mucous membranes, mouth and tongue,'the lining of the peritoneum, the lymphatics and sensory organs.
Materials and Methods:
The (3AED, 17 beta-oestradiol and tamoxifen were obtained from Sigma Chemical Company (St. Louis, Missouri). The aAED
and Flutamide were obtained from Steriloid, Inc. (Walton, New Hampshire) and Schering Corparation (Kenilworth, New Jersey), respectively. All steroids except aAED were dissolved in ethanol. aAED was dissolved in DMSO:ETHOL (1:1 v/v). Stock solutions were filtered and kept at 4°C. For testing, stock solutions were diluted in media immediately before use. The final concentration of vehicle was always < 0.2~ in all samples, and this concentration had no significant cytotoxic effect on the human breast cell cancer cell line designated ZR-75-1 (American Type Culture Collection) as determined by trypan blue exclusion.
The ZR-75-1 cell line (passage 89) was obtained from the American Type Culture Collection (Rockville, Maryland). The cells were cultured in RPMI-1690 medium containing 10~ heat inactivated fetal calf serum, 200 ~M L-glutamine, 10 NM HEPES, 1 . 5 U/ml penicillin, and 1 . 5 E.cg/ml streptomycin in 5 0, C02 at 37°C. The cells were passaged twice weekly.
Cell _,r~wth for tests na:
Cells were first seeded at initial density of 1x105 cells per ml in quadruplicates in 29 multi-well flat bottom plates (Costar). Cells were then allowed to adhere and grow in phenol red-free RPMI 69 media supplemented with 10 ~ heat-inactivated fetal calf serum (FCS) , 200 /.cM L-glutamine, 10 mM HEPES, 2. 5 WO 97/37662 PCTlUS97/05849 U/ml penicillin and 2.5 ~g/ml streptomycin in 5o COZ at 37°C.
After 48 hours, four wells were sacrificed and counted to determine plating efficiency. In the remaining wells, medium was removed by aspiration and cells in each well were exposed to the media containing the specified steroid. The controls containing only medium or medium with vehicle were also prepared. In all samples, medium was changed every 48 hours.
At the pre-established time-point. Cells were removed by trypsinization and washed. Cell number and viability were determined by trypan blue exclusion using a hemocytometer.
Parallel cultures were also run to determine cell proliferation.
Cell Proliferation Assays:
For cell proliferation assays, cell suspensions were prepared by trypsinization of cells from cultures prepared in accord with methods described above. Cell viability was determined by trypan blue exclusion. The cells were then seeded in flat-bottom 96-well microtiter plates at a density of 2X103 cells/well and were allowed to rest for 48 hours in order to adhere. Non-adherent dead cells were removed by aspiration. Cells were then grown in media without phenol red.
Some of the media contained supplements as indicated above.
The active agents or vehicle control were added to the media.
The cells were then grown for six days. Media was changed on the samples every 48 hours. On day 6, cells were pulsed with 1 E.cCi[3J]-thymidine for the last 6 hours of incubation before harvesting onto glass filter using a HPD cell harvester (Cambridge Technology, Watertown, Massachusetts) and counted on a KLB scintillation counter.
Initial tests were carried out to determine the optimal (maximal) dose of aAED required to inhibit growth of the ZR-75-1 cells in in vitro as determined by tritiated tymidine incorporation. It was found that treatment of ZR-75-1 cells with aAED at doses ranging from 3.I3 r~M to 50 r~M lead to a biphasic effect on the growth of ZR-71-1 cells when compared to vehicle control after 6 days. The aAED stimulated proliferation of ZR-75-1 cells at concentrations between 6.25 and 12.5 nM (P<0.01) when compared with the vehicle control.
At concentration of 25 nM or greater, the aAED significantly inhibited the growth of ZR-75-1 cells, and this anti-proliferative effect occurred in a dose and time-dependent manner at half-maximal (50 riM) and maximal dose (100 r~M) levels. To ensure that inhibition was not due to cytotoxicity, cell count and viability were assessed by trypan blue exclusion. The addition of the l7aAED was not toxic to the cells. As opposed to aAED, the (3AED alone at 100 nM
concentrations did not have any antiproliferative effect on the growth of the ZR-75-1 cells.
Example 1:
ZR-75-1 cells (2X103) were treated as described above over a six day period with differing concentrations of aAED or with vehicle-only cultures. The medium was changed every 48 hours.
Cells were pulsed with 3H-thymidine for the last six hours of incubation.
Results showed increasing proliferation at 6.25 nM with decreasing cell proliferation at 25 nM and marked decrease at concentrations of 50 nM concentrations.
Example 2:
Cells were treated as in Example 1, except that in some samples a combination of l7aAED and aAED were used. The concentration of the aAED varied while the concentration of the ~iAED in the samples containing the combination of agents remained constant at 2.5 nM. Cells were pulsed with 3H
thymidine for the last six hours.
The proliferation of cells in cultures containing aAED in the presence of (3AED showed decreased proliferation at all concentrations of aAED.
Example 3:
Effects of aAED on growth of ZR-75-1 cells in the presence of estradiol was studied. Cells treated with increasing concentration of aAED in the presence or absence of 1 nM
concentration of estradiol over a 6 day period were studied.
Cells were treated with increasing concentration of aAED in the presence if 1 r~M concentration of estradiol over a 6 day period, with medium changed every 48 hours. Cells were pulsed with 3H-thymidine for the last six hours of incubation. At higher doses of aAED the aAED suppressed proliferation even in the presence of estradiol. Hence, estradiol can not effectively overcome the antiproliferative activity of aAED on this human breast cancer cell line.
Example 4:
Effects of aAED on growth of ZR-75-1 cells in the presence of Flutamide, an antiandrogen, were studied using the process of Example 3 except that estradiol was replaced with Flutamide.
At concentrations of __>6.25 nM concentrations of aAED, the antiproliferative effects were greatly enhanced in the presence of Flutamide. Hence, Flutamide appears to act synergistically with aAED to produce antiproliferative effects. Hence, the administration of aAED with antiandrogens, especially in treating estrogen-dependent malignancies such as breast cancer, should be considered particularly advantageous treatment option.
Example 5:
Example 5 was again studied in the presence of RU486.
Again, it was shown that at effective concentrations it was possible to lower dosage of RU486 in the presence of effective amounts of .1 ~1 concentrations of a.AED with 0.5 uM con-centration of RU486 .there was synergistic action to decrease proliferation of cells. This synergism was shown to be even greater at RU486 concentrations of 1 ~cM. This combination of active agents would be especially useful for treatment of tumors which are dependent on estrogen or progestrone Example 6:
Preparation for instillation:
Ingredient ow/w D 0.01 ' 5 polypropylene glycol 13.0 $
Water 86.5 Example 7:
Preparation for intravenous injection:
Ingredient Amount 1 mg.
Ethanol 5 ml.
Phosphate buffered saline Add to 1000 ml.
Example 8:
Effect of aAED on growth of lymphoid neoplasm (P388D1 cells obtained from the American Type Culture Collection) in the presence of RU486 at concentration of 0.5 ,uM and 1.0 ,uM was studied in accord with the methods described above . It was found that the use of aAED in combination with RU486 resulted in increased effectiveness over use of one agent.
Example 9:
Effects of aAED at doses of 50 r~M and 100 r~M doses on murine macrophage myeloma cells (RAW 264.7, obtained from the American Type Culture Collection) was studied. At both 50 nM
and 100 nM levels there was significant inhibition of pro-liferation.
Example 10:
Preparation for instillation into the bladder for treatment of bladder cancer:
Ingredient Amount 10 mg DMSO 100 ml half-normal saline 900 ml.

Example 11:
Water, 100 ml, is mixed with 7 g. (3-hydroxypropyl cyclo-dextrin and 1 mg aAED. Fill ampules with the solution and sterilize. This preparation may be added to solutions for 5 administration to the mucosa, for oral administration, or for parenteral administration.
Example 12:
The cyclodextrin/aAED preparation is prepared as above.
The material is freeze-dried and placed in sterile ampules.
10 The resulting powder may be placed in vials. The contents of the vials may then be snorted into the nasal cavity. It is also appropriate to dissolve the contents of the vials and place in solution for intravenous or topical application, including for infusion into a wound site. It may also be applied by spraying or sponging into the operative site such as the abdominal or thoracic cavity.
Example 13:
The preparation of Example 12 is diluted with 100 ml water. The preparation is sprayed into the abdominal cavity during and after removal of a colon malignancy.
aAED may be delivered to or through the skin by any means, including subcutaneous or intradermal injection or topical application. One means of topical application is the use of skin patches impregnated with the active agent. This means of delivery is advantageous since it is non-invasive and easily administered by relatively unskilled care providers.
Example 14:
Capsules of a formulation of aAED for oral administration is prepared by mixing 2 mg. orAED, 15 mg. Starch and 5 mg.
Magnesium stearate. The capsules are administered twice a day to achieve a daily dosage of 1-50 mg./da.
The compositions of the invention may be administered intrathecally either at the spinal level or into the cisterna magna.
When aAED, its esters or ethers are administered orally, WO 97!37662 PCT/US97l05849 it is necessary that the active agents be protected from destruction and absorption in the upper gastro-intestinal tract. The active agents are most effective when the period of exposure to the mucosa of the intestinal tract is increased.
Hence use of capsules containing the active agents in formulations that effect slow release in the intestine are appropriate.
The active agents may also be used in veterinary medicine for treatment of animals suffering from tumors. For purposes of such treatment, the ocAED may be added to the chow of the animals.
The sterile solutions may be administered to the lung either by bronchioscopic means or by mist which may be under pressure.
Patched for administration of aAED may be formulated as adhesive patches containing the active agent. For example, the patch may be a discoid in which a pressure-sensitive silicone adhesive matrix containing the active agent may be covered with a non-permeable backing. The discoid may either contain the active agent in the adhesive or may be attached to a support made of material such as polyurethan foam or gauze that will hold the active agent. When patches are used in treating animals, the area must be shaved or plucked. In all instances, the area to which the patch is applied should be cleaned carefully before application.
Example 15:
A patch composed of trilaminate of an adhesive matrix sandwiched between a non-permeable backing and a protective covering layer is prepared in the following manner:
To a pressure-sensitive silicone adhesive composition BIOPSA"" q7-2920 (Dow Corning Corporation, Midland, Michigan, U.S.A.) In cyclohexane (50$ w/v) is added sufficient aAED to provide a .5$ aAED composition. The adhesive is applied to a polyester film to provide in successive layers about 2 mg. Of active agent per cm2. Patches should be covered with a protective layer which will be removed before application.
Patches may be prepared containing permeation enhancers such as cyclodextrin, butylated hydroxyanisole, or butylated hydroxytoluene.
The active agents may be administered to the mucosa of oral, pharyngeal or nasal cavity by tablet or lozenge.
The antiproliferative agents taught herein may be used in conjunction with other active agents such as vinca alkaloids, nucleic acid inhibitors, platinum agents, interleukin-2, interferons, alkylating agents, antimetabolites, corticosteroids, DNA intercalating agents, anthracyclines and ureas. Examples of specific agents, in addition to those exemplified herein, include hydroxyurea, 5-fluorouracil, anthramycin, asparaginase, bleomycin, dactinomycin, dacabazine, cytarabine, busulfan, thiotepa, lomustine, mechlorehamine, cyclophosphamide, melphalan, mechlorethamine, chlorambucil, carmustine, 6-thioguanine, methotrexate, etc.

Claims (23)

WHAT WE CLAIM IS:

1. ~A composition of matter comprising a tumor proliferation-inhibiting effective amount of .alpha.AED or an ester or ether thereof of the formula:
wherein R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-carbons, or alkenyl of 2,4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, in a form appropriate for oral administration.

2. ~A composition of claim 1 which is a tablet.

3. ~A composition of claim 1 wherein the composition is in the form of a capsule.

4. ~A composition of claim 1 wherein the composition is a liquid.

5. ~A sterile composition of matter comprising a tumor proliferation-inhibiting effective amount of .alpha.AED or an ester or ether thereof of the formula:

wherein R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4.
carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, in a sterile solution appropriate for parenteral administration.

6. A composition of claim 5 in single dosage form in a container.

7. A composition of claim 5 in an isotonic solution.

8. A composition of claim 5 containing the .alpha.AED in a cyclodextrin inclusion complex.

9. A composition of matter comprising a tumor proliferation inhibiting effective amount of a compound of the formula:

wherein either R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, on a solid support.

10. A composition of claim 9 which is a patch for application to the skin.

11. A composition of claim 9 which is a sponge.

12. A composition of matter comprising a tumor proliferation inhibiting effective amount of a compound of the formula:

wherein either R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, in a pharmaceutically acceptable carrier wherein said composition contains, additionally, as an active agent, a second anti-cancer drug.

13. A composition of claim 12 wherein the second anti-cancer drug is a antiandrogen or antiestrogen.

14. A composition of claim 12 wherein the second anticancer drug is a area.

15. A composition of claim 12 wherein the second anticancer drug is an alkylating agent.

16. A composition of claim 12 wherein the second anticancer drug is an antimetabolite.

17. A composition of matter of claim 12 wherein the second anticancer drug is a DNA intercalating agent.

18. A composition of claim 12 wherein the second anticancer drug is an interferon.

19, A composition of claim 12 wherein the second anticancer drug is a nucleic acid inhibitor.

20. A composition of claim 12 wherein the second anticancer drug is a vinca alkaloid.

21. A composition of claim 21 wherein the second anticancer drug is RU486.

22. Use of a compound of formula:

wherein either R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, for the manufacture of a medicament for the acceleration of the aging of cells and programmation of the death of cells.

23. Use of a compound of formula:

wherein either R may be H, alkenyl of 2-8 carbons, alkyl of 1-8 carbons, phenylalkyl of 1-4 carbons, phenyl or COR2, wherein R2 is H; alkyl of 1-8 carbons, alkenyl of 2-8 carbons, phenylalkyl wherein the alkyl has 1-4 carbons (including benzyl) or phenyl, and any phenyl moiety may have up to three substituents chosen from among hydroxy, carboxy of 1-4 carbons, halo, alkoxy of 1-4 carbons, alkyl of 1-4 carbons, or alkenyl of 2-4 carbons and wherein any alkyl may be a straight chain, branched chain, or the alkyl may be wholly or partially cyclized, and its esters and ethers, in the acceleration of the aging of cells and programmation of the death of cells.