CA2251241C - Apoptosis inhibitor - Google Patents

Abstract

The present invention provides an apoptosis inhibitor containing a 15R-isocarbacyclin derivative or a 15-deoxy-isocarbacyclin derivative, which can be manufactured easily and in low cost by means of chemical synthesis.

Description

APOPTOSIS INHIBITOR
Field of The Invention The invention of the present application relates to an apoptosis inhibitor and, more particularly, it relates to a novel apoptosis inhibitor containing isocarbacyclin derivative which has been known as a ligand specific to prostacyclin receptors of the central nervous type as an effective component and having an excellent inhibiting action to apoptosis of nerve cells, etc.
Prior Art Apoptosis is a kind of genetically programmed cell death and, morphologically, it takes place according to the following processes. Thus, aggregation of nucleus; cell contraction;
vacuole formation; smoothening of cell surface; expansion of intercellular intervals; liberation of cells from the surroundings; fragmentation of cells (apoptosis corpuscles);
and phagocytosis by macrophage, etc. It has been also known that, biochemically, nucleosome unit of DNA is fragmented by endonuclease activity into DNA of base length of 180-200 (Immunology Today, 7:115-119, 1986; Science, 245:301-305, 1989) .
At present, it has been clarified that, in addition to physiological functions such as development/differentiation and turnover of normal tissues and cells, this apoptosis participates in certain diseases such as inflammation and reduction of lymphocytes caused by ischemic nerve cell death after cerebral infarction, etc., involution of carcinoma, action of radioactive ray and anticancer agent and infection of virus such as AIDS. Thus, development of drugs for controlling them (i.e., apoptosis inhibitors and apoptosis inducers) has been expected to give birth to drugs based upon new action mechanism in the broad areas including central nervous system as well as cancer, aging, etc.
With regard to substances and factors for inducing apoptosis, toxicity of neurotransmitters such as glucocorticoid and glutamic acid, irradiation of radioactive ray, NK cells, killer cells, tumor necrosis factor (TNF), cytokines such as lymphotoxin (LT), etc. have been reported.
It has been also reported that cycloheximide which is a protein synthesis inhibitor or actinomycin D which is an RNA synthesis inhibitor induces apoptosis to human leukemia cells HL-60.
Further, anti-Fas monoclonal antibody to Fas antigen which is a cell membrane molecule participating in apoptosis of immune cells has been prepared recently and various investigations have been conducted for application of anti-Fas antibody to pharmaceuticals.
On the other hand, substances which inhibit the interleukin 1 converting enzyme,basic fibroblast growth factor (bFGF), etc. have been reported as factors for inhibiting apoptosis. It has been also known that bcl-2 related gene products inhibit apoptosis and have a macrobiotic function for cells. However, all of those apoptosis inhibiting factors are derived from living organisms and, up to now, no apoptosis inhibitor obtained by industrial means such as chemical synthesis has been known yet.
On the other hand, during the course of investigation of physiological action of brain function in detail, the inventors of the present application have found various isocarbacyclins acting as specific ligands to prostacyclin receptor of the central nerve system and have filed patent applications already [Japanese PatentApplicationNos. 051,589/96 (JP-A245,498/96) , 243,122/96 (JP-A 87608/98), 260,957/96 (JP-A 101610/98) and 160,320/97]. As a result of further investigation on the physiological activity of those substances, several of them have been found to show a significant apoptosis inhibiting effect.
Accordingly, an object of the invention of the present application is to offer a novel apoptosis inhibitor which can be manufactured by means of chemical synthesis in large quantities and also in low cost by a further development of the above-mentioned findings by the present inventors.

Summary of The Invention The first invention of this application is an apoptosis inhibitor containing a 15R-isocarbacyclin derivative represented by the following formula (1) as an effective component.
.... ~, r~
s (1) W

-oH OH
(in the formula, Rl is hydrocarbon chain having 1-6 carbon atom(s); and RZ is hydrogen atom or a protective group) In the apoptosis inhibitor of the first invention, it is a preferred embodiment that the 15R-isocarbacyclin derivative is 15R-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin represented by the following formula (2) or methyl ester thereof .
I'I1I1L
(2) The second invention is an apoptosis inhibitor containing a 15-Deoxy-isocarbacyclin derivative represented by the following formula (3) as an effective component.
,.""
Z
(3) y OH
(in the formula, R1 is hydrocarbon chain having 1-6 carbon atom(s); and RZ is hydrogen atom or a protective group) In the apoptosis inhibitor of the second invention, it is a preferred embodiment that the 15-Deoxy-isocarbacyclin derivative is 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin represented by the following formula (4) or methyl ester thereof.
""." , (4) In the above-mentioned formulae (1) and (3), the protective group which constitutes RZ is pharmaceutically acceptable salt, ester, etc. and its example is alkyl group which constitutes methyl ester, ethyl ester, etc.
Embodiments of the present invention will now be further S

illustrated as hereunder.
Brief Description of The Drawings Figs. 1 and 2 are graphs showing the inhibitory effect of the tested samples on apoptosis of hippocampal neuron.
Fig. 3 is a chemical formulae showing the synthetic process of Deoxy-TIC.
Fig. 4(A) and (B) are graphs showing uptake of labeled isocarbacyclin derivatives in whole brain and thalamus, respectively.
Detailed Description of The Invention An example of the 15R-isocarbacyclin derivative which is an effective component of the first invention is 15R-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin (hereinafter, may be referred to as "15R-TIC" ) or methyl ester thereof , and can be prepared by the method disclosed in JP-A 245,498/96 filed by the present inventors.
The 15-Deoxy-isocarbacyclin derivative which is an effective component of the second invention is, for example, 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin (hereinafter, may be referred to as "Deoxy-TIC") or methyl ester thereof, and can be prepared by the method disclosed in the Japanese Patent Application No. 160, 320/97 filed by the present inventors.

The apoptosis inhibitor of the present invention can be made into pharmaceutical preparations containing any of those isocarbacyclin derivatives. In the case of manufacturing a pharmaceutical preparation for applying on central nervous system, it is preferred to manufacture the preparation with the methyl ester or the like of those isocarbacyclin derivatives.
This is because that, as shown in the following Referential Example 2 , such a methyl ester or the like is easily taken up into the brain and is converted to an isocarbacylin derivative such as 15R-TIC or Deoxy-TIC in the brain thereby achieving an inhibiting effect on neural apoptosis. For the purpose of inhibiting the apoptosis in somatic cells, isocarbacyclin derivative such as 15R-TIC or Deoxy-TIC can be used as an effective component of a preparation. In addition to such an effective component, the apoptosis inhibitor of the present invention may further contain known apoptosis inhibiting substances.
The apoptosis inhibitor of the present invention may be administered to human or other animals in a form of common pharmaceutical preparations. For example, it may be administered to human or other animals by means of intravenous injection, subcutaneous injection or oral administration.
The apoptosis inhibitor of the' present invention may be made into pharmaceutical. preparations containing not less than 5 a mol/kg of isocarbacyclin derivative together with other components. Examples of said other components are those which are commonly used in the field of manufacture of pharmaceuticals such as diluting agents and vehicles including fillers, bulking agents, binders, moisturizers, disintegrating agents, surface-active agents and lubricants. With regard to a dosage form, various forms may be selected depending upon the object of therapy and representative examples thereof are tablets, pills, diluted powder, liquid, suspension, emulsion, granules, capsules, suppositories and injections (in liquid, suspension, etc. ) .
For example, in the case of preparing the injections, it is preferred that liquid, emulsion and suspension are sterilized and are isotonic to blood. Examples of the applicable diluent are water, ethyl alcohol, propylene glycol, ethoxylated isostearyl alcohol, polyoxylated isostearyl alcohol and polyoxyethylene sorbitan fatty acid esters. In that case, it is also possible to add salt, glucose, glycerol, etc. in such an amount that is satisfactory to prepare an isotonic solution. It is further possible to compound auxiliary solubilizers, buffers, analgetizing agent, etc.
therewith.
If necessary, the pharmaceutical preparations in each of the above-mentioned forms may be further compounded with commonly used coloring agents, preservatives, perfumes, condiments, sweeteners, etc. Other pharmaceutically effective components may be compounded therewith as well.
The present invention will be illustrated in detail and in a specific manner by the following examples although the present invention is not limited to those examples.
Example 1 Effect of 15R-TIC and Deoxy-TIC on hippocampal neuron apoptosis induced by a high-oxygen incubation was tested.
(1) Samples Isocarbacyclin was a pure sample supplied from Teijin Co. , Ltd. 15S-TIC was prepared in accordance with the method disclosed in Example 3 of JP-A 245,498/96 by using 15S-16-m-tolyl-17,18,19,20-tetra-nor-carbacyclin which is also disclosed in Example 2 of the same Publication JP-A245,498/96.
The 15R-TIC was prepared by the same method as disclosed in Example 3 of the Publication JP-A 245,498/96. bFGF was the commercially available one.
(2) Procedures Hippocampal region was cut out from the fetal rat brain which was aseptically isolated from a pregnant rat (Wistar strain) of 20 days age and the hippocampal region was dispersed into neurons by shaking culture for 30 minutes in a PBS (Ca-and Mg-free) containing DL-cysteine-(0.2 mg/ml), bovine serum albumin (0.2 mg/ml), glucose (5 mg/ml), DNase I (0.010 and papain (9 units/ml). After that, each neuron was planted at the density of 5 x 105 cells/cm2 on a 24-well plate (coated with polyethyleneimine) filled with a DME/F-12 medium (containing 5~ equine serum and 5~ fetal bovine serum) and incubated for two days in a 5~ carbon dioxide gas incubator (9~ oxygen) . After that, the culture medium was substituted with an DME/F-12 medium (containing 5 ~.t g/ml of transferrin, 5 a g/ml of insulin and 5 nM progesterone) wherefrom serum was removed, then each sample to be tested was added to the medium, after 30 minutes, transferred into 50~k oxygen. After incubating in 50~ oxygen for 48 hours, the survival rate of the neurons was measured.
The neurons in 9~ oxygen were also added each sample and the survival rate was measured.
With regard to the survival rate, the neurons were immobilized by 4$ paraformaldehyde, stained with anti-MAP2 antibody and colored by DAB and the cells positive to MAP2 were counted from the 200-fold enlarged picture whereupon the survival rate was calculated.
(3) Results Fig. 1 shows the survival rate of hippocampal neurons incubated in 9~S or 50~ oxygen where bFGF was added at a concentration of 50 nM and other samples were at 5,~c M. The number of survived neurons in 9$ oxygen was used as a control.
When isocarbacyclin or 15S-TIC was added, the survival rate of hippocampal neuron was greatly reduced by apoptosis induced by a high-oxygen condition as same as under the control condition.

On the contrary, when 15R-TIC or Deoxy-TIC was added, an increase in the survival rate of neurons was observed to the same or even better extent as compared with the case of bFGF
which was known as an apoptosis inhibitor in this experimental system.
Fig. 2 shows the dose-dependent effects of the tested samples on hippocampal neural apoptosis. In this figure, the survival rate of neurons in 9~ oxygen was used as a control, and the survival rate of neurons to which each sample being added was expressed as the mean ~ S.D. (n=4). As being clear from Fig. 2, while isocarbacyclin and 15S-TIC did not have any effect on neuroprotection in the range of concentrations tested, both 15R-TIC and Deoxy-Ticshow the dose-dependentinhibitory effect on neural apoptosis, and the survival rate at 3000 nM was equivalent to the control . IC50 values were ca . 30 nM and 300 nM for Deoxy-TIC and 15R-TIC, respectively.
From these results, it has been now ascertained that 15R-TIC and Deoxy-TIC, the effective components of the apoptosis inhibitor of the present invention exhibit an excellentinhibiting effect to apoptosis of cells, particularly to apoptosis of neurons.
Example 2 Effect of 15R-TIC. on hippocampal CA1 pyramidal neurons apoptosis induced by ischemic damage in mongolian gerbils was tested in vivo.
To examine whether 15R-TIC can prevent neuronal death in vivo, using an osmotic mini-pomp, 15R-TIC solution was continuously infused for 7 days (started 2 days before the transient ischemia and ended at 5 days) into the left lateral ventricle of gerbils that had been subjected to a transient forebrain ischemia for 3 minutes. Survival number of CA1 pyramidal neurons was assessed by counting neurons with cell bodies larger than 10 ~c m in the Nissl-staining sections.
Administration of 15R-TIC blocked the loss of CA1 pyramidal neurons induced by forebrain ischemia. The number of neurons in the 15R-TIC-infused gerbils (984~ 299 in a 20 ~c m thick section, n=4) was comparable to that in ischemia-free contral gerbils (1061~220, n=4) . However, the number of GAl pyramidal neurons in vehicle-infsed gerbils (142~28, n=4) was significantly less than that of the control (without ischemic treatment) or 15R-TIC-infused gerbils. Pyramidal neurons in the vehicle-infused gerbils has progressively degenerated, with the nuclei having shrunk and the alignment of cells destroyed.
These results indicate that 15R-TIC acts as an effective neuronal survival-promoting factor in vivo, as well as in vitro data shown in Examples 1 and 2.

Referential Example 1 In accordance with the description of Example 1 of the Japanese Patent Application No. 160,320/97, Deoxy-TIC was synthesized as follows, in accordance with Fig 3.
<1> Synthesis of the aldehyde compound (6) A solution of (triphenylphosphoranylidene) acetaldehyde (21.8 mg, 71.6 ~.c mol) in benzene (1.5 ml) was placed in a 10-ml Skulenk tube, then a solution of the above aldehyde compound (5) (23.0 mg, 65.3 ~c mol) in benzene (1.5 ml) was added thereto and the mixture was heated to reflux for 20 hours . The reaction mixture was cooled, the solvent was evaporated therefrom and the residue was purified by means of an Si02 chromatography using 2:1 and 1:1 mixtures of hexane and ethyl acetate to give an aldehyde compound (6) (14.8 mg, 61~). The Rf value in the .
TLC was 0.55 (1:1 mixture of hexane and ethyl acetate).
<2> Synthesis of the carbonate compound (7) A solution of the above aldehyde (6) (7.6 mg, 20.2 a mol) in methanol (1.0 ml) was placed in a 10-ml round-bottom flask, then CeCl~~H20 (10 mg, 27 ~.c mol) and NaBH4 (2 mg, 53 ~,c mol) were added thereto and the mixture was stirred for five minutes . After that, ethyl acetate and water were added to the reaction mixture. Extraction was conducted for three times using ethyl acetate as a solvent. The organic phases were combined, dried over MgS09 and filtered and the filtrate was concentrated in vacuo. The resulting crude product was placed in a 10-ml round-bottom flask and dissolved in CH2C12 (2.0 ml) .
To this solution were added DMAP (37 .0 mg, 0 .303 mmol) and methyl chloroformate (0.015 ml, 0.194 mmol) followed by stirring for four hours . After that, an aqueous solution of NaHC03 was added thereto and the mixture was extracted with ethyl acetate. The organic phases were combined, dried over Na2SOq and filtered and the filtrate was concentrated in vacuo. This was purified by means of an Si02 chromatography using a 4 :1 mixture of hexane and ethyl acetate to give the above-mentioned carbonate compound (7) (8.0 mg, 91$). Rg in TLC was 0.42 (2:1 mixture of hexane and ethyl acetate).
<3> Synthesis of the adduct compound (9) A solution of tris (dibenzylideneacetone)-dipalladium (O) -chloroform adduct (2 . 1 mg, 2 . 0 ~.c mol ) and 1, 2 bis(diphenylphosphino)ethane (1.6 mg, 4.0 ~.c mol) in THF (1.0 ml) was placed in a 20-ml Skulenk tube. To this solution was added a solution of the above carbonate compound (7) (8.0 mg, 18.3 ~.t mol) and disulfone (7.6 mg, 19.7 ~t.c mol) in THF (1.0 ml) and the mixture was stirred for 15 minutes. The reaction mixture was poured into an aqueous solution of NHqCl followed by extracting with ethyl acetate. The organic phases were combined, dried over MgSOq and filtered and the filtrate was concentrated in vacuo. This was purified by means of an Si02 chromatography using 2:,1, 1:1 and 1:2 mixtures of hexane and ethyl acetate to give a desired adduct (9) (9.6 mg, 70~). Rg in TLC was 0.27 (1:1 mixture of hexane and ethyl acetate).
<4> Synthesis of the compound (10) : 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin methyl ester Mg (10 mg, 0.4 mmol) was placed in a 10-ml round-bottom flask and then a solution of the above adduct (9) (7.5 mg, 10.0 ~c mol) in methanol (1.5 ml) was added followed by stirring for three hours. An aqueous solution of HC1 (1N) was added to the reaction mixture followed by extracting with ethyl acetate.
The organic phases were combined, dried over MgSOq and filtered and the filtrate was concentrated in vacuo. The resulting crude product was placed in a 10-ml round-bottom flask and dissolved in a 9:1 mixture (2.0 ml) of acetic acid and water.
After stirring for 40 hours, ethyl acetate was added to the solution followed by washing with an aqueous solution of NaHC03 .
The organic phase was dried over Na2SOq and filtered and the filtrate was concentrated in vacuo. This was then purified by means of an Si02 chromatography using a 3:1 mixture of hexane and ethyl acetate to give a desired compound (10), i.e., 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin methyl ester (2.2 mg, 58~) . Rg in TLC was 0.6 (1:1 mixture of hexane and ethyl acetate).
<5> Synthesis of the compound (4): 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin A solution of the above compound (10) (1.0 mg, 2.6 mol) in methanol (0.5 ml) was placed in a 10-ml test tube, an aqueous solution (3N, 0.2 mol) of NaOH was added thereto and the mixture was stirred at room temperature for 12 hours.
NaHC03 was added thereto and then ethyl acetate and water were further added. The pH was adjusted to 3. The organic phase was separated and the aqueous phase was extracted with ethyl acetate. The organic phases were combined, dried over MgS04 and filtered and the filtrate was concentrated in vacuo. This was purified by means of an Si02 (0.5 g) chromatography using a 10:1 mixture of CH2C12 and methanol to give the desired compound (4), the 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin (0.9 mg, 94~) . Rg in TLC was 0.39 (9:1 mixture of CH2C12 and methanol).
Referential Example 2 Uptake of the isocarbacyclin derivative into brain was measured by means of positron emission tomography (PET).
(1) Samples 11C-Labeled 15R-TIC methyl ester (RTA) was prepared by labeling the methyl group carbon at 16-m-tolyl group in the 15R-TIC methyl ester with 11C. 11C-Labeled methyl of 15R-TIC
(RTC) was prepared by labeling the methyl group carbon at methyl group in the 15R-TIC methyl ester with 11C. The 15R-TIC methyl ester was prepared by the same method as in Example 2 of JP-A
245498/96.
(2) Procedures A tracer amount (not more than 0.2E.cg/kg) of the above mentioned sample was intravenously injected to an adult rhesus monkey (body weight being about 8 kg) and the behavior of the sample in the brain from the intravenous injection until 60 minutes thereafter was imaged by means of a PET device. ROI
(region of interest) was calculated from said image and the uptake amount was calculated by the following formula.
Uptake Amount = [(Calculated Weight of ROI in Brain)/(Body Weight)] . [(RI Counts Accumulated in ROI)/(Administered RI Counts)]
(3) Results Fig. 4(A) shows the incorporated amount of each of the labeled compounds in the whole brain and Fig. 4(B) shows the incorporated amount in the thalamus. RTA and RTC showed equivalent pass through blood-brain-barrier (Fig. 4A and B), but RTA retained in the brain further than RTC, indicating the retention of deesterified 11C-Labelled 15R-TIC in the brain.
In other words , RTA could serve as a prodrug for 15R-TIC . It is clear from those results that the 15R-TIC methyl ester systemically administered has been confirmed to be efficiently transferred into the brain. Since thelabeled compound remains in the brain for not shorter than 60 minutes after the administration, it has been suggested that the 15R-TIC methyl ester (a precursor to 15R-TIC) is converted to 15R-TIC in the brain to bind to prostacyclin receptor and that its apoptosis inhibiting action is achieved in the brain.
According to the present invention, a novel apoptosis inhibitor, of which ingredient is a compound available easily and in low cost by means of chemical synthesis, is provided.
It has been clarified by PET study that the isocarbacyclin derivative, an effective component of the apoptosis inhibitor, passes through the blood-brain-barrier and acts on the central nervous system and, accordingly, the inhibitor is particularly effective for the therapy of various diseases caused by neural apoptosis.

Claims (4)

1. An apoptosis inhibitor containing 15R-isocarbacyclin derivative represented by the following formula (1) as an effective component.
(in the formula, R1 is hydrocarbon chain having 1-6 carbon atom(s); and R2 is hydrogen atom or a protective group)

2. The apoptosis inhibitor of claim 1, wherein the 15R-isocarbacyclin derivative is 15R-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin represented by the following formula (2) or methyl ester thereof.

3. An apoptosis inhibitor containing 15-Deoxy-isocarbacyclin derivative represented by the following formula (3) as an effective component.

(in the formula, R1 is hydrocarbon chain having 1-6 carbon atom(s); and R2 is hydrogen atom or a protective group)

4. The apoptosis inhibitor of claim 3, wherein the 15-deoxy-isocarbacyclin derivative is 15-Deoxy-16-(m-tolyl)-17,18,19,20-tetranorisocarbacyclin represented by the following formula (4) or methyl ester thereof.