CA2243669A1 - Composition for accelerating plant seed germination, plant growth and methods using same - Google Patents
Composition for accelerating plant seed germination, plant growth and methods using same Download PDFInfo
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- CA2243669A1 CA2243669A1 CA002243669A CA2243669A CA2243669A1 CA 2243669 A1 CA2243669 A1 CA 2243669A1 CA 002243669 A CA002243669 A CA 002243669A CA 2243669 A CA2243669 A CA 2243669A CA 2243669 A1 CA2243669 A1 CA 2243669A1
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- 239000000203 mixture Substances 0.000 title claims abstract description 18
- 230000008635 plant growth Effects 0.000 title claims abstract description 15
- 230000007226 seed germination Effects 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 31
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 claims description 16
- 235000008696 isoflavones Nutrition 0.000 claims description 16
- 150000002515 isoflavone derivatives Chemical class 0.000 claims description 14
- 235000021374 legumes Nutrition 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 6
- 230000002708 enhancing effect Effects 0.000 claims description 6
- 244000299507 Gossypium hirsutum Species 0.000 claims description 5
- 244000061176 Nicotiana tabacum Species 0.000 claims description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims 1
- 240000003768 Solanum lycopersicum Species 0.000 claims 1
- 244000068988 Glycine max Species 0.000 description 13
- 230000035784 germination Effects 0.000 description 13
- 235000010469 Glycine max Nutrition 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 230000012010 growth Effects 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 235000012343 cottonseed oil Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 101100516786 Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) nodD1 gene Proteins 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- ZQSIJRDFPHDXIC-UHFFFAOYSA-N daidzein Chemical compound C1=CC(O)=CC=C1C1=COC2=CC(O)=CC=C2C1=O ZQSIJRDFPHDXIC-UHFFFAOYSA-N 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 229930003949 flavanone Natural products 0.000 description 2
- 150000002208 flavanones Chemical class 0.000 description 2
- 235000011981 flavanones Nutrition 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 101150100830 nodD gene Proteins 0.000 description 2
- 230000024121 nodulation Effects 0.000 description 2
- 230000008121 plant development Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 208000005156 Dehydration Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000589157 Rhizobiales Species 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 239000002975 chemoattractant Substances 0.000 description 1
- 230000035605 chemotaxis Effects 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 235000007240 daidzein Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000459 effect on growth Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- TZBJGXHYKVUXJN-UHFFFAOYSA-N genistein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC(O)=C2C1=O TZBJGXHYKVUXJN-UHFFFAOYSA-N 0.000 description 1
- 235000006539 genistein Nutrition 0.000 description 1
- 229940045109 genistein Drugs 0.000 description 1
- ZCOLJUOHXJRHDI-CMWLGVBASA-N genistein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 ZCOLJUOHXJRHDI-CMWLGVBASA-N 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 101150017145 nod gene Proteins 0.000 description 1
- 101150083279 nodD2 gene Proteins 0.000 description 1
- 108010003099 nodulin Proteins 0.000 description 1
- 230000005305 organ development Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Saccharide Compounds (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to agriculture. More specifically, the invention relates to plant seed germination and plant growth. Even more specifically, the present invention relates to compositions which accelerate plant seed germination and plant growth of numerous types of plants and to methods using same.
Description
TITLE OF THE INVENTION
COMPOSITION FOR ACCELERATING PLANT SEED
GERMINATION, PLANT GROWTH AND METHODS USING SAME.
FIELD OF THE INVENTION
The present invention relates to agriculture. More specifically, the invention relates to plant seed germination and plant growth. Even more specifically, the present invention relates to compositions which accelerate plant seed germination and plant growth of numerous types of plants and to methods using same.
BACKGROUND OF THE INVENTION
SoyaSignal~ is a product, designed by Bios Agriculture Inc., to initiate soybean nodulation and nitrogen fixation under cool soil temperatures. SoyaSignal has been tested in the last 4 years and over twenty different locations in both Canada and USA. As the SoyaSignal technology increased, the final soybean grain yield by 11.1 %, farmers in both countries have accepted this product.
Initial recognition between 8. japonicum and soybean involves exchange of molecular signals (Stacey et al, 1995). Legume roots secrete phenolic compounds (Dakora & Philips, 1996; Peters &
Verma, 1990), largely from the area of root hair emergence, which act as chemo-attractants to (brady)rhizobia (Nap & Bisseling, 1990), and activate the nod genes. Flavones, isoflavones, and flavanones have been identified as the inducing molecules for (brady)rhizobial chemotaxis and for expression of nod genes, e.g. genistein, daidzein and several related compounds in soybean (Peters & Verma, 1990). These plant-to-
COMPOSITION FOR ACCELERATING PLANT SEED
GERMINATION, PLANT GROWTH AND METHODS USING SAME.
FIELD OF THE INVENTION
The present invention relates to agriculture. More specifically, the invention relates to plant seed germination and plant growth. Even more specifically, the present invention relates to compositions which accelerate plant seed germination and plant growth of numerous types of plants and to methods using same.
BACKGROUND OF THE INVENTION
SoyaSignal~ is a product, designed by Bios Agriculture Inc., to initiate soybean nodulation and nitrogen fixation under cool soil temperatures. SoyaSignal has been tested in the last 4 years and over twenty different locations in both Canada and USA. As the SoyaSignal technology increased, the final soybean grain yield by 11.1 %, farmers in both countries have accepted this product.
Initial recognition between 8. japonicum and soybean involves exchange of molecular signals (Stacey et al, 1995). Legume roots secrete phenolic compounds (Dakora & Philips, 1996; Peters &
Verma, 1990), largely from the area of root hair emergence, which act as chemo-attractants to (brady)rhizobia (Nap & Bisseling, 1990), and activate the nod genes. Flavones, isoflavones, and flavanones have been identified as the inducing molecules for (brady)rhizobial chemotaxis and for expression of nod genes, e.g. genistein, daidzein and several related compounds in soybean (Peters & Verma, 1990). These plant-to-
2 bacteria signal compounds cause expression of the bacterial nod (also nol and noe genes) very rapidly (only a few minutes after exposure) and at very low concentrations (10~'to 10-a M) (Peters et al., 1986). Generally this is through an interaction with nodD, which activates the common nod genes, although the situation may be more complex, as is the case in 8.
japonicum, where nodD,, nodD2 and nodVVVare involved (Gillette & Elkan 1996; Stacey 1995).
Among the products of the nod genes (Spaink, 1995;
Stacey, 1995) induced by the plant phenolic signal molecules are various enzymes involved in the synthesis of a series of lipo-chitooligosaccharides (LCOs). These LCOs act as bacterium-to-plant signals, inducing expression of many of the early nodulin genes (Long, 1989). This results in root hair deformation (including curling) cortical cell division leading to initiation of nodule meristems, secretion of additional nod gene inducers, and initiation of infection threads (Verma, 1992).
These bacterium-to-plant signals exert a powerful influence over the plant genome and, when added in the absence of the bacteria, can induce the formation of root nodules (Truchet et al., 1991 ). Thus, the bacteria-to-plant signals can, without the bacteria, induce all the gene activity for nodule organogenesis (Denarie et al., 1996; Heidstra & Bisseling, 1996).
The relationship between environmental variables, such as low RZT and pH, and the interplay of molecular signals has only recently become a subject of investigation. For example, soybean plant has less synthesis abilities of isoflavones under cool soil temperature, whereas the higher isoflavone concentration is needed to turn on the nod genes of 8. japonicum (Zhang and Smith 1995 and 1997). To overcome the negative effect of low temperature on the early event of symbiotic
japonicum, where nodD,, nodD2 and nodVVVare involved (Gillette & Elkan 1996; Stacey 1995).
Among the products of the nod genes (Spaink, 1995;
Stacey, 1995) induced by the plant phenolic signal molecules are various enzymes involved in the synthesis of a series of lipo-chitooligosaccharides (LCOs). These LCOs act as bacterium-to-plant signals, inducing expression of many of the early nodulin genes (Long, 1989). This results in root hair deformation (including curling) cortical cell division leading to initiation of nodule meristems, secretion of additional nod gene inducers, and initiation of infection threads (Verma, 1992).
These bacterium-to-plant signals exert a powerful influence over the plant genome and, when added in the absence of the bacteria, can induce the formation of root nodules (Truchet et al., 1991 ). Thus, the bacteria-to-plant signals can, without the bacteria, induce all the gene activity for nodule organogenesis (Denarie et al., 1996; Heidstra & Bisseling, 1996).
The relationship between environmental variables, such as low RZT and pH, and the interplay of molecular signals has only recently become a subject of investigation. For example, soybean plant has less synthesis abilities of isoflavones under cool soil temperature, whereas the higher isoflavone concentration is needed to turn on the nod genes of 8. japonicum (Zhang and Smith 1995 and 1997). To overcome the negative effect of low temperature on the early event of symbiotic
3 nitrogen fixation, the SoyaSignal technology has been designed (Canadian application number 2,179,879). The major components of SoyaSignal are isoflavones and LCOs.
While the effect of isoflavones and LCOs on nodulation, nitrogen fixation, growth and protein yield of legumes, such as soybean, has been shown to compensate for stress conditions of the environment, their effect on growth of non-legumes is unknown. In fact, the role of such legume-specific-related molecules on non-legumes has never been assessed on non-legume plants.
There thus remains a need to assess the effect of isoflavones and/or LCOs on plant germination and/or growth of plants and especially of non-legume plants.
The present invention seeks to meet these and other needs.
The present description refers to a number of documents, the content of which is herein incorporated by reference.
SUMMARY OF THE INVENTION
The invention concerns a composition for enhancing seed germination of plants and growth thereof. More specifically, the present invention relates to a composition of SoyaSignal which can increase soybean seed germination and plant growth in addition to acting as a trigger to initiate soybean symbiotic nitrogen fixation. Surprisingly, the compositions of the present invention act not only on legumes such as soybeans but on plants in general as exemplified with corn and cotton.
In a particular set of experiments, a composition of the present invention
While the effect of isoflavones and LCOs on nodulation, nitrogen fixation, growth and protein yield of legumes, such as soybean, has been shown to compensate for stress conditions of the environment, their effect on growth of non-legumes is unknown. In fact, the role of such legume-specific-related molecules on non-legumes has never been assessed on non-legume plants.
There thus remains a need to assess the effect of isoflavones and/or LCOs on plant germination and/or growth of plants and especially of non-legume plants.
The present invention seeks to meet these and other needs.
The present description refers to a number of documents, the content of which is herein incorporated by reference.
SUMMARY OF THE INVENTION
The invention concerns a composition for enhancing seed germination of plants and growth thereof. More specifically, the present invention relates to a composition of SoyaSignal which can increase soybean seed germination and plant growth in addition to acting as a trigger to initiate soybean symbiotic nitrogen fixation. Surprisingly, the compositions of the present invention act not only on legumes such as soybeans but on plants in general as exemplified with corn and cotton.
In a particular set of experiments, a composition of the present invention
4 comprising isoflavones and LCOs was shown to significantly enhance early plant growth.
Broadly, the present invention further relates to compositions comprising isoflavones and LCOs for enhancing seed germination of plants and growth thereof.
The invention in addition relates to methods for enhancing the germination and/or growth of plants comprising an incubation of a seed of a plant with an effective amount of isoflavones and LCOs for a sufficient time and under conditions which enable an increased germination of the seed and/or an increased growth of the plant.
The applicant was the first to show that isoflavones and LCOs had an effect on non-legume germination and plant growth.
While the germination and/or plant growth enhancing capabilities of the composition of the instant invention are demonstrated with corn and cotton, they are applicable to other plants such as, for example, potato tubers.
Similarly, while the present invention has been demonstrated with low RZT, the composition and methods of the present invention should find use in stimulating germination and/or growth under environmental field conditions. Non-limiting examples thereof include water stress, pH and high temperature.
It shall also be understood that compositions other than SoyaSignal, comprising isoflavones (and/or flavones and/or flavanones) and LCOs, are also encompassed within the scope of the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS
Having thus generally described the invention, reference will now be made to the accompanying drawings, showing by way of illustration a preferred embodiment thereof, and in which:
Broadly, the present invention further relates to compositions comprising isoflavones and LCOs for enhancing seed germination of plants and growth thereof.
The invention in addition relates to methods for enhancing the germination and/or growth of plants comprising an incubation of a seed of a plant with an effective amount of isoflavones and LCOs for a sufficient time and under conditions which enable an increased germination of the seed and/or an increased growth of the plant.
The applicant was the first to show that isoflavones and LCOs had an effect on non-legume germination and plant growth.
While the germination and/or plant growth enhancing capabilities of the composition of the instant invention are demonstrated with corn and cotton, they are applicable to other plants such as, for example, potato tubers.
Similarly, while the present invention has been demonstrated with low RZT, the composition and methods of the present invention should find use in stimulating germination and/or growth under environmental field conditions. Non-limiting examples thereof include water stress, pH and high temperature.
It shall also be understood that compositions other than SoyaSignal, comprising isoflavones (and/or flavones and/or flavanones) and LCOs, are also encompassed within the scope of the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS
Having thus generally described the invention, reference will now be made to the accompanying drawings, showing by way of illustration a preferred embodiment thereof, and in which:
5 Figure 1 shows the germination enhancing effect of a composition according to the present invention.
Other objects, advantages and features of the present invention will become more apparent upon reading of the following non-restrictive description of preferred embodiments with reference to the accompanying drawing which is exemplary and should not be interpreted as limiting the scope of the present invention.
DESCRIPTION OF THE PREFERRED EMBODIMENT
Surprisingly, we have shown that the addition of SoyaSignal, in the presence of isoflavones and LCOs accelerates the germination of corn seeds, whereas isoflavone solutions alone do not.
Presumably this effect was due to the LCOs produced by the presence of isoflavones and 8. japonicum cells. When the seedlings were harvested (still at the mesocotyl stage) they were 44% longer and 33%
as heavier, than the plants which had not received the genistein-8.
japonicum treatment (Figure 1 ). !n addition, we have shown that the rate of cottonseed germination is also accelerated by the application of SoyaSignal. The germination rate of cotton seeds treated with SoyaSignal at the rate of 0.66 ml/kg seed increased by 145% compared to those control seeds that only treated with pure water. Both the corn and cotton work were conducted at low temperatures, 15°C for corn seeds and 17.5°C for cottonseeds.
Other objects, advantages and features of the present invention will become more apparent upon reading of the following non-restrictive description of preferred embodiments with reference to the accompanying drawing which is exemplary and should not be interpreted as limiting the scope of the present invention.
DESCRIPTION OF THE PREFERRED EMBODIMENT
Surprisingly, we have shown that the addition of SoyaSignal, in the presence of isoflavones and LCOs accelerates the germination of corn seeds, whereas isoflavone solutions alone do not.
Presumably this effect was due to the LCOs produced by the presence of isoflavones and 8. japonicum cells. When the seedlings were harvested (still at the mesocotyl stage) they were 44% longer and 33%
as heavier, than the plants which had not received the genistein-8.
japonicum treatment (Figure 1 ). !n addition, we have shown that the rate of cottonseed germination is also accelerated by the application of SoyaSignal. The germination rate of cotton seeds treated with SoyaSignal at the rate of 0.66 ml/kg seed increased by 145% compared to those control seeds that only treated with pure water. Both the corn and cotton work were conducted at low temperatures, 15°C for corn seeds and 17.5°C for cottonseeds.
6 From our field trial this year, the time of tasselling of sweet corn treated with SoyaSignal (planted on May 6 on the Experimental Farm of McGill University, Quebec) was 1 to 2 days earlier compared to untreated plants. Soybean seeds that received SoyaSignal (planted on June 22 in Martinsville, Illinois) emerged 8 hour earlier compared to control seeds while the first trifoliar fully expanded 1 day earlier. At the agronomy farm of Purdue University, IN. soybean plants planted in early June and observed in early July had already grown to one stage further in their development (V6) compared to the control plants (V5). In a farmer trial (in Jackson, Illinois), plants that received SoyaSignal had much more nodule on the secondary roots and were 10%
taller than untreated plants.
It is still not fully understood why SoyaSignal works on plant in this way. Our general understanding of the role of LCOs in signalling during the establishment of the legume-rhizobia symbiosis was described above. When added to the appropriate legume, LCOs can cause the induction of nodule meristems. Two previous publications have shown that LCOs can induce cell cycle activities in other systems. De Jong et al. (1993) showed improved embryogenesis in a carrot embryogenesis system. Rohrig et al. (1995) showed increased cell division in a suspension of tobacco protoplasts at concentrations as low as 10'SM. The possible explanation of our observation may be as the following. First, LCOs might be normal signal molecules in higher plants, so that exogenously supplying them simply increases their levels and, therefore, the activity of the things they would normally regulate. Second, there may be an endogenous class of signal molecules which have play important roles in plant development, and have a conformation similar to
taller than untreated plants.
It is still not fully understood why SoyaSignal works on plant in this way. Our general understanding of the role of LCOs in signalling during the establishment of the legume-rhizobia symbiosis was described above. When added to the appropriate legume, LCOs can cause the induction of nodule meristems. Two previous publications have shown that LCOs can induce cell cycle activities in other systems. De Jong et al. (1993) showed improved embryogenesis in a carrot embryogenesis system. Rohrig et al. (1995) showed increased cell division in a suspension of tobacco protoplasts at concentrations as low as 10'SM. The possible explanation of our observation may be as the following. First, LCOs might be normal signal molecules in higher plants, so that exogenously supplying them simply increases their levels and, therefore, the activity of the things they would normally regulate. Second, there may be an endogenous class of signal molecules which have play important roles in plant development, and have a conformation similar to
7 those of LCOs. Without being limited to a particular theory, the present invention is the first to have identified a germination and/or plant growth promoting effect of isoflavones and LCOs on non-legume plants.
The crops, such as soybean, corn and cotton evolved in relatively warm climates and, as a result, have high base temperatures for germination, being 10°C for both soybean and corn and 14°C
for cotton, respectively. These high base germination temperatures lead to slow emergence after planting, resulting in slow leaf ground cover early in the season, which in turn leads to poorer early season light interception, poorer competition with weeds (and therefore greater need for herbicide application) and increased soil erosion during heavy rainfall events. Using SoyaSignal as a plant growth regulator can partially overcome the negative effects of environmental stress conditions, such as low soil temperature on crop seed germination and plant development.
These will improve the production of tropical and subtropical origin crops in temperate zone and may extend their production into other short season areas.
Although the present invention has been described hereinabove by way of preferred embodiments thereof, it can be modified, without departing from the spirit and nature of the subject invention as defined in the appended claims.
The crops, such as soybean, corn and cotton evolved in relatively warm climates and, as a result, have high base temperatures for germination, being 10°C for both soybean and corn and 14°C
for cotton, respectively. These high base germination temperatures lead to slow emergence after planting, resulting in slow leaf ground cover early in the season, which in turn leads to poorer early season light interception, poorer competition with weeds (and therefore greater need for herbicide application) and increased soil erosion during heavy rainfall events. Using SoyaSignal as a plant growth regulator can partially overcome the negative effects of environmental stress conditions, such as low soil temperature on crop seed germination and plant development.
These will improve the production of tropical and subtropical origin crops in temperate zone and may extend their production into other short season areas.
Although the present invention has been described hereinabove by way of preferred embodiments thereof, it can be modified, without departing from the spirit and nature of the subject invention as defined in the appended claims.
8 REFERENCES
Dakora FD & Phillips DA, 1996, Physiol & Molec Path 49:1-20.
De Jong AJ, Heidstra R, Spaink H, Hartog MV, Meijer EA, Hendriks T, Lo Shiavo F, Terzi M, Bisseling T, Van Kammen A, & De Vries SC, 1993, Plant Cell 5:615-620.
Denarie J, Debelle F & Prome J-C, 1996, Annu Rev Biochem 65:503-535.
Fry SC, Aldington S, Hetherington R & Aitken J, 1993, Plant Phys 103:1-5.
Gillette WK & Elkan G, 1996, J Bact 178:2757-2766.
Heidstra R & Bisseling T, 1996, New Phytol 133:25-43.
Long SR, 1989, Cell 56:203-214.
Nap JP and Bisseling T, 1990, Science 250:948-954.
Peters NK and Verma DPS, 1990, Mol. Plant-Microbe Interact. 3:4-8.
Peters NK, Frost JW & Long SR, 1986, Science 233:977-980.
Rohrig H, Schmidt J, Walden R, Czaja I, Mikiasevics E, Wieneke U, Schell J & John M, 1995, Science 269:841-843.
Spaink HP, 1995, Annu Rev Phytopath 33:345-368.
Stacey G, 1995, FEMS Microbiol Lett 127:1-9.
Truchet G, Roche P, Lerouge P, Vasse J, Camut S, de Billy F, Pome J-C
& Denarie J, 1991, Nature 351:670-673.
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Claims
1. An agricultural composition comprising isoflavones and LCOs for enhancing plant seed germination and/or plant growth of a plant together with an agriculturally suitable carrier.
2. The composition of claim 1, wherein said plant is a non-legume.
3. The composition of claim 2, wherein said plant is selected from the groups selected from graminae, solonacae and malvacae.
4. The composition of claim 3, wherein said plant is selected from the group comprising corn, cotton, tomato, potato and tobacco.
5. A method to enhance plant seed germination and/or plant growth of a plant comprising an incubation of said seed and/or plant with an agriculturally effective amount of the agricultural composition of
claim 1.
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002243669A CA2243669A1 (en) | 1998-07-21 | 1998-07-21 | Composition for accelerating plant seed germination, plant growth and methods using same |
| MXPA01000755A MXPA01000755A (en) | 1998-07-21 | 1999-07-21 | Composition for accelerating seed germination and plant growth. |
| CA002338108A CA2338108C (en) | 1998-07-21 | 1999-07-21 | Composition for accelerating seed germination and plant growth |
| AU48924/99A AU4892499A (en) | 1998-07-21 | 1999-07-21 | Composition for accelerating seed germination and plant growth |
| US09/744,129 US6979664B1 (en) | 1998-07-21 | 1999-07-21 | Composition for accelerating seed germination and plant growth |
| AT99932579T ATE230928T1 (en) | 1998-07-21 | 1999-07-21 | METHOD OF LIPOCHITOOLIGOSACCHARIDES FOR ACCELERATING PLANT SEED GERMINATION, EMERGING AND/OR PLANT GROWTH |
| EP99932579A EP1098567B1 (en) | 1998-07-21 | 1999-07-21 | Use of lipochitooligosaccharides containing compositions for accelerating seed germination, seedling emergence and/or plant growth |
| PCT/CA1999/000666 WO2000004778A1 (en) | 1998-07-21 | 1999-07-21 | Composition for accelerating seed germination and plant growth |
| DK99932579T DK1098567T3 (en) | 1998-07-21 | 1999-07-21 | Composition for accelerating seed germination and plant growth |
| DE69904939T DE69904939D1 (en) | 1998-07-21 | 1999-07-21 | METHOD OF LIPOCHITOOLIGOSACCHARIDES FOR ACCELERATING PLANT SEEDS, GROWTH AND / OR PLANT GROWTH |
| ES99932579T ES2192389T3 (en) | 1998-07-21 | 1999-07-21 | USE OF COMPOSITIONS CONTAINING LIPOQUITOOLIGOSACARIDOS TO ACCELERATE THE GERMINATION OF THE SEEDS, THE EMERGENCY OF THE SEEDS AND / OR THE GROWTH OF THE PLANTS. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002243669A CA2243669A1 (en) | 1998-07-21 | 1998-07-21 | Composition for accelerating plant seed germination, plant growth and methods using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2243669A1 true CA2243669A1 (en) | 2000-01-21 |
Family
ID=4162676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002243669A Abandoned CA2243669A1 (en) | 1998-07-21 | 1998-07-21 | Composition for accelerating plant seed germination, plant growth and methods using same |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6979664B1 (en) |
| EP (1) | EP1098567B1 (en) |
| AT (1) | ATE230928T1 (en) |
| AU (1) | AU4892499A (en) |
| CA (1) | CA2243669A1 (en) |
| DE (1) | DE69904939D1 (en) |
| DK (1) | DK1098567T3 (en) |
| ES (1) | ES2192389T3 (en) |
| MX (1) | MXPA01000755A (en) |
| WO (1) | WO2000004778A1 (en) |
Cited By (3)
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| WO2005087005A1 (en) * | 2004-03-18 | 2005-09-22 | Agribiotics Inc. | Isoflavonoid compounds and use thereof |
| US7262151B2 (en) | 2001-07-06 | 2007-08-28 | Mcgill University | Methods and compositions for production of lipo-chito oligosaccharides by rhizobacteria |
| US10117432B2 (en) | 2004-03-18 | 2018-11-06 | Novozymes Bioag A/S | Isoflavonoid compounds and use thereof |
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| US6258749B1 (en) * | 2000-02-22 | 2001-07-10 | The Dow Chemical Company | Methods for treating plants and enhancing plant growth using polyacylglycosides and/or polyalkylglycosides and formulations for same |
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| CA2959417C (en) * | 2007-01-09 | 2019-10-01 | Novozymes Biologicals Holding A/S | Lipo-chitooligosaccharide and flavonoid combination for enhanced plant growth and yield |
| CN101578044B (en) * | 2007-01-09 | 2013-08-14 | 默克专利股份有限公司 | Lipochitooligosaccharide combined composition for promoting plant growth and increasing yield |
| JP5502493B2 (en) * | 2007-01-16 | 2014-05-28 | シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト | Agrochemical combination |
| BRPI0817934B1 (en) * | 2007-10-16 | 2017-03-21 | Merck Patent Ges Mit Beschränkter Haftung | composition and methods for synergistically improving plant yield comprising a combination of rhizobium leguminosarum genistein, daidzein and lipo-chitosol |
| WO2010049751A1 (en) * | 2008-10-29 | 2010-05-06 | Institut National De La Recherche Agronomique | Lipochitooligosaccharides stimulating arbuscular mycorrhizal symbiosis |
| WO2012078656A1 (en) | 2010-12-06 | 2012-06-14 | Novozymes North America, Inc. | Methods of hydrolyzing oligomers in hemicellulosic liquor |
| EP2683239A1 (en) * | 2011-03-10 | 2014-01-15 | Bayer Intellectual Property GmbH | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
| RU2611199C2 (en) | 2011-03-31 | 2017-02-21 | Новозаймз Байолоджикалз, Инк | Competitive and effective strains of bacteria |
| US20120329135A1 (en) | 2011-06-23 | 2012-12-27 | Agrinos AS | Process for Making Chitin Derivatives |
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| CN106211857A (en) | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| WO2013040366A1 (en) * | 2011-09-14 | 2013-03-21 | Novozymes Biologicals Holdings A/S | Use of lipochito-oligosaccharides and/or chito oligosaccharides in combination with phosphate-solubilizing microorganisms to enhance plant growth |
| US8946119B2 (en) | 2011-09-23 | 2015-02-03 | Novozymes Bioag A/S | Chitooligosaccharides and methods for use in enhancing soybean growth |
| IN2014CN02815A (en) * | 2011-09-23 | 2015-07-03 | Novozymes Bioag As | |
| WO2013044214A1 (en) | 2011-09-23 | 2013-03-28 | Novozymes Biologicals Holdings A/S | Combinations of lipo-chitooligosaccharides and methods for use in enhancing plant growth |
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| CN102388884A (en) * | 2011-10-28 | 2012-03-28 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composition used for improving crop yield as well as use and method thereof |
| AR089596A1 (en) * | 2011-12-16 | 2014-09-03 | Novozymes Biologicals Inc | BRADYRHIZOBIUM CEPAS |
| US20130255338A1 (en) | 2012-03-27 | 2013-10-03 | Agrinos, AS | Microbial Composition Comprising Liquid Fertilizer and Processes for Agricultural Use |
| BR112015006606A2 (en) * | 2012-09-27 | 2017-07-04 | Novozymes Bioag As | composition, method for enhancing the growth of a plant or part of the plant, and seed |
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| CN107852892A (en) * | 2017-11-21 | 2018-03-30 | 云南省烟草农业科学研究院 | A kind of method for reducing disease, accelerating tobacco seed emergence |
| US12232494B2 (en) | 2018-01-08 | 2025-02-25 | Novozymes Bioag A/S | Aqueous compositions comprising solubilzed lipo-chitooligosaccharides |
| WO2019147660A1 (en) * | 2018-01-23 | 2019-08-01 | Novozymes Bioag A/S | Method of enhancing seed germination under abiotic stress with chitin oligosaccharides |
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| US4367609A (en) | 1980-07-28 | 1983-01-11 | Coated Seed Limited | Use of microorganisms in conjunction with seeds |
| FR2646425B1 (en) | 1989-04-26 | 1991-08-30 | Neosystem Sa | SYNTHETIC PEPTIDES OF THE CONJUGATE OF UBIQUITINE AND HISTONE H2A |
| US5549718A (en) * | 1990-04-06 | 1996-08-27 | Centre National De La Recherche Scientifique (C.N.R.S.) | Substance with lipo-oligosaccharide structure capable of acting as plant-specific symbiotic signals, processes for producing them and their applications |
| FR2660658A1 (en) | 1990-04-06 | 1991-10-11 | Centre Nat Rech Scient | SUBSTANCES OF OLIGOSACCHARIDE STRUCTURE CAPABLE OF PLAYING THE ROLE OF PLANT-SPECIFIC SYMBIOTIC SIGNALS, THEIR PRODUCTION PROCESSES AND THEIR APPLICATIONS. |
| US5175149A (en) * | 1991-10-04 | 1992-12-29 | The University Of Tennessee Research Corporation | Pentasaccharide phytohormones and methods for their use |
| US5321011A (en) | 1991-10-04 | 1994-06-14 | The University Of Tennessee Research Corporation | Pentasaccharide phytohormones and methods for their use |
| FR2692896B1 (en) | 1992-06-29 | 1994-09-09 | Institut Rech Agronomique | Broad spectrum host rhizobiaceae nodulation signals. |
| GB9601110D0 (en) | 1996-01-19 | 1996-03-20 | Cocking Edward C D | Method of inducing nitrogen fixation in plants |
| CA2179879C (en) | 1996-06-25 | 2004-02-24 | Donald L. Smith | Composition for enhancing grain yield and protein yield of legumes grown under environmental conditions that inhibit or delay nodulation thereof |
-
1998
- 1998-07-21 CA CA002243669A patent/CA2243669A1/en not_active Abandoned
-
1999
- 1999-07-21 AU AU48924/99A patent/AU4892499A/en not_active Abandoned
- 1999-07-21 DE DE69904939T patent/DE69904939D1/en not_active Expired - Lifetime
- 1999-07-21 ES ES99932579T patent/ES2192389T3/en not_active Expired - Lifetime
- 1999-07-21 MX MXPA01000755A patent/MXPA01000755A/en unknown
- 1999-07-21 AT AT99932579T patent/ATE230928T1/en not_active IP Right Cessation
- 1999-07-21 DK DK99932579T patent/DK1098567T3/en active
- 1999-07-21 WO PCT/CA1999/000666 patent/WO2000004778A1/en active IP Right Grant
- 1999-07-21 US US09/744,129 patent/US6979664B1/en not_active Expired - Lifetime
- 1999-07-21 EP EP99932579A patent/EP1098567B1/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7262151B2 (en) | 2001-07-06 | 2007-08-28 | Mcgill University | Methods and compositions for production of lipo-chito oligosaccharides by rhizobacteria |
| WO2005087005A1 (en) * | 2004-03-18 | 2005-09-22 | Agribiotics Inc. | Isoflavonoid compounds and use thereof |
| EA014817B1 (en) * | 2004-03-18 | 2011-02-28 | Иэмди Кроп Байосайенс Кэнада Инк. | Isoflavonoid compounds and use thereof |
| US10117432B2 (en) | 2004-03-18 | 2018-11-06 | Novozymes Bioag A/S | Isoflavonoid compounds and use thereof |
| US11800868B2 (en) | 2004-03-18 | 2023-10-31 | Novozymes Bioag A/S | Isoflavonoid compounds and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1098567B1 (en) | 2003-01-15 |
| DE69904939D1 (en) | 2003-02-20 |
| ES2192389T3 (en) | 2003-10-01 |
| EP1098567A1 (en) | 2001-05-16 |
| WO2000004778A1 (en) | 2000-02-03 |
| ATE230928T1 (en) | 2003-02-15 |
| US6979664B1 (en) | 2005-12-27 |
| DK1098567T3 (en) | 2003-06-02 |
| MXPA01000755A (en) | 2002-04-08 |
| AU4892499A (en) | 2000-02-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |