CA2237639C - Procede pour quantifier une substance biologique dans un echantillon - Google Patents

Procede pour quantifier une substance biologique dans un echantillon Download PDF

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Publication number
CA2237639C
CA2237639C CA2237639A CA2237639A CA2237639C CA 2237639 C CA2237639 C CA 2237639C CA 2237639 A CA2237639 A CA 2237639A CA 2237639 A CA2237639 A CA 2237639A CA 2237639 C CA2237639 C CA 2237639C
Authority
CA
Canada
Prior art keywords
plate
incubation
wells
sample
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2237639A
Other languages
English (en)
Other versions
CA2237639A1 (fr
Inventor
Andrew J. Croteau
Mark W. Pierson
David E. Townsend
Ali Naqui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BioControl Systems Inc
Original Assignee
BioControl Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/606,229 external-priority patent/US5700655A/en
Priority claimed from US08/746,054 external-priority patent/US5985594A/en
Application filed by BioControl Systems Inc filed Critical BioControl Systems Inc
Publication of CA2237639A1 publication Critical patent/CA2237639A1/fr
Application granted granted Critical
Publication of CA2237639C publication Critical patent/CA2237639C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Ce procédé sert à détecter une substance biologique dans un échantillon. Ce procédé consiste à liquéfier l'échantillon (si nécessaire) et à verser l'échantillon ainsi liquéfié dans la plaque d'incubation. Cette plaque d'incubation possède une surface horizontale généralement plate et cette surface est divisée en au moins 20 puits creux. Chaque puits est conçu pour contenir une aliquote d'un liquide et sa grandeur et sa forme ainsi que sa constitution en un matériau approprié sont telles qu'il peut contenir l'aliquote grâce à sa tension de surface. Tout liquide excédentaire provenant de l'échantillon liquéfié est versé depuis la surface de la plaque. Ledit procédé consiste ensuite à incuber cette plaque d'incubation jusqu'à ce que la présence ou l'absence de la substance biologique soit déterminée.
CA2237639A 1995-11-13 1996-11-12 Procede pour quantifier une substance biologique dans un echantillon Expired - Lifetime CA2237639C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US08/557,529 1995-11-13
US55752995A 1995-11-14 1995-11-14
US08/606,229 US5700655A (en) 1995-11-14 1996-02-23 Method for quantification of biological material in a sample
US08/606,229 1996-02-23
US08/746,054 US5985594A (en) 1995-11-14 1996-11-06 Method for quantification of biological material in a sample
US08/746,054 1996-11-06
PCT/US1996/018119 WO1997018455A1 (fr) 1995-11-13 1996-11-12 Procede pour quantifier une substance biologique dans un echantillon

Publications (2)

Publication Number Publication Date
CA2237639A1 CA2237639A1 (fr) 1997-05-22
CA2237639C true CA2237639C (fr) 2011-01-11

Family

ID=43449754

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2237639A Expired - Lifetime CA2237639C (fr) 1995-11-13 1996-11-12 Procede pour quantifier une substance biologique dans un echantillon

Country Status (1)

Country Link
CA (1) CA2237639C (fr)

Also Published As

Publication number Publication date
CA2237639A1 (fr) 1997-05-22

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Effective date: 20161114