CA2234164C - Novel orthosomycins from micromonospora carbonacea - Google Patents

Abstract

Compounds of the formulae (see formula I) R1 R2 R3,R3' R4 R5 R6 evernino micrin H OH OH,H OMe Cl Me Compd A CI OH OH,H OMe Cl Me Compd B H OH =O OMe Cl Me Compd D H OMe OH,H OMe Cl Me Compd E H OH OH,H OH H Me Compd F H OH OH,H OMe H Me Compd G H OH OH,H OMe Cl H;

or wherein R7=CH3; R8=CH3; (Compound C) or R7=C(O)H; R8 =H (Compound H), or

Description

This invention relates to novel orthosomycins from Micromonospora Carbonacea,their use and compositions containing them.
SUMMARY OF THE INVENTION
Compounds A, B, C, D, E, F, G, H and J have been isolated from the fermentation broth of the microorganism Micromonospora Carbonacea var Africana designated SCC 2146. These compounds were identified as orthosomycins. These compounds are antibacterial agents. A component from the culture, compound A, was found to be the most potent antibacterial agent.
The invention relates to novel antibacterial compounds A, B, C, D, E, F, G, H and J, and their preparation, and to compositions containing such compounds.
This invention also relates to a fermentation broth of the microorganism Carbonacea var Africana, and the component parts thereof obtainable by cultivation of a pure culture of Micromonospora Carbonacea var Africana.
The invention relates to the microorganism Micromonospora Carbonacea var Africana. Another aspect of the invention is directed to the antibiotic complex produced by cultivating a strain of Micromonospora Carbonacea var Africana .
in a pH and temperature controlled aqueous nutrient medium having assimilable sources of carbon and nitrogen under controlled submerged aerobic conditions until a composition of matter having substantial antibiotic activity Is produced. A
major component of the culture of the present invention is antibiotic 13-384, component l, as disclosed in U.S.P. 4,587,968. (Another major component of the culture is the corresponding nitroso analog.) However, the present invention claims other compounds of the culture as described below.

The present invention is also related to an ant~iotic composition comprising a pharmaceutically aooeptabte carrier and an antibiotically effective amount of one or more c~npounds selected from the group consisting of compounds A, B, C, D, E, F, G, H and ,D. The present invention S is also related to a method of trig a bacterial infection which comprises administering an antibiotically effective amount of one or more compounds selected from the group consisting of compounds A, B, C, D, E, F, G, H and J.
DESCRIPTION OF THE FIGURES
Figures 1, 2, 4, 5, 6, 7 and B are proton NMR spectra for Compounds A, B, E, F, G, H, and J, respectively. Figure 3 is a proton NMR spectra for a mixture Compounds C and D.
DETAILED DESCRIPTION OF THE INVENTION

One mL frozen culture of Jhlicromonospora car6onaoea subspecies Africans, strain PF6-3 was transferred into a 300 mL Erlenmeyer shake 25 flask containing 100 mL of seed medium. The medium composition (g2) was as follows: beef extract (Difco) 3, Tryptone (D'rfco) 5, Cerelose (CPC, 2001 ) 1, potato dextrin {Avebe, V~PD-650) 24, yeast extract (Universal, Tastone) 5, calcium carbonate (Pfizer, Albaglos) 1, and scone defoamer (Union Carbide, SAG-47~, 3096 suspension) 0.3 mt.A.. The flask was 30 incubated 48 hours at 30oC with agitation (300 rpm, 1 inch stroke), and 30 mL of the culture were transferred to 6x2 L flasks, each containing 500 mL
of the same seed medium. Folbwing 48 hours of incubation as before, the 3 L culture was transferred into an inoculation fermenter conta~ing 300 L
of the same medium for an additions! 24 hours of cultivation. Finally, the 35 c~tents of the inoculation fertnen#er were transferred to a larger fermenter containing 10,000 L of production medium. The composition of the prod~tion medium (g~L) was as follows: yeast extract 5, meat peptone (Maroon, type PS) 6, Cerelose 22, coin steep powder (Marcor) 2, potato dextrin 60, boiled linseed oil (Kleenstrip) 4, calcium carbonate 4, cobalt chloride-6 H20 (Mallindcrodt) 0.002, silicone defoamer 0.5 mL/L The fermentation was conducted at 36'aC for 120 to '140 hours under aeration and agitation maintaining the dissolved oxygen between 50 to 100°.6 saturation. The fermentation was carried out with 80-240 standard cx:bic feet per minute air flow for about 120 -130 hours.
tSOLATtON
The fermentation broth was cooled to about 25~C. One half of the fermentation broth was transferred to a separate vessel, agitated and adjusted with 2 N NaOH to pH 10.5. A 300 L XAD-7 resin (Rohm 8~ Haas non functional acrylic ester polymeric adsorbent) was charged to the fermentation broth and agitated for 0.5 hours. The pH was lowered to 9.25 and ag'~tatec! for 3.5 hours. The pH was further lowered to 7.00, and the resin was separated form the broth by screening. Tap water was used to wash the resin free from both broth and mycelia. The second half of the fermentation broth was processed in the same way.
About 300 L of adsorbed resin was charged to a 500 L
tapered column containing 100 L deionized water. The resin was washed with upflow. After dropping the aqueous level to the resin bed, the resin was washed downftow with 900 L of deionized water. The antibiotic was stated from the resin downftow by charging the column with 900 L ethyl acetate (pre-washed with 140 L of 0.1 M, sodium phosphate monobasic buff, adjusted to pH 8 with sodium hydroxide) at 10 Llminutes. The etuate was collected in 150 to 200 L cuts. The antibiotic com~ex containing cuts were combined and extracted with 140 L of 0.1 M sodium phosphate monobasic, adjusted to pH 6 with sodium hydroxide, then with 2 x 60 L
de'fonized water. The ethyl acetate layer was vacuum concentrated at less than 30~C to one tenth the original volume (about 50 L) with azeotropic WO 97/13777 ~'CT/US96/15750 distillation ofi residual water. The concentrate was precipitated into 100 L
of heptane (2 volumes). The precipitate was filtered and dried at about 25~C
in a vacuum oven usthg a n~rogen bleed to give 52 to 5.5 kg of crude material (2.6 to 3.1 kg of antibiotic complex).
With agitation,10 to 11 kg of the crude ant~iotic complex were dissolved in 10 volumes ofi 80J20 ethyl acetate-acetone to oxidize the nitroso component of the complex of the antibio~c 13-384. Two kilograms of sodium bicarbonate and 50 g ofi vanadyl acetyacetonate catalyst were added. 5.5 L tertbutyl hydroperoxide, (3 M solution in 2,2,4-trimethyl pentane) were added slowly over 0.5 hour while the temperature was maintained at 25 to 30~C. The progress ofi the oxidation was monitored by by HPLC (high pressure liquid chromatography). Additional catalyst was added when needed and agitation of the reaction mixhrre was continued until the read was complete.
INITIAL PURIFICATION
Approximately 2.5 kg of crude oxidized material was dissolved in 9 L of ethyl acetate and applied to the head of a 10 foot by 1 foot diameter column packed with 70 kg of bare irregular silica gel (70 to 250 pm) in isopropyl acetate (The solvent can also be 80/20 ethyl acetatelheptane). The column was eluted with isopropyl acetate at a flow rate of 5 to 7 Uminute at 35 pounds per square inch gauge. Fractions 1 , 2, and 3 were collected as 200 L cuts. The antibiotic components were monitored by HPLC and TLC . The solvent used for TLC chromatographies was 9:1 methytene chloride-methyl alcohol. Fractions 5 to 11 which were enriched th the main antibiotic component were combined and vacuum concentrated to about 6 L at less than 30~C. The main cut was isolated by adding the concentrate to 2 volumes of heptane with agitation. The precipitate was filtered and dried in a vacuum oven at about 25~C with a nitrogen bleed to obtain 1.2 kg of product. The head cut fractions 3 and 4 which were enriched in impurities were prcocessed in a simlar manner to obtain 0.4 kg of product. The tail cut fractions 12 to 15 which were enriched in impurities, wars prcocessed in a similar manner to obtain 0.2 kg of product.

WO 97/13777 PCT/~TS96/15750 Further Purification of the Antibiotic Complex The purification was accomplished in a two step chromatographic procedure. Both head (ID 33285-104-2) and tail cuts (ID 33285-104-1) obtained from initial purification were first chromatographed under medium pressure conditions with diol-bonded silica gel (40--63 p. irregular media). The column was equilibrated with a ternary mixture of CH2CI2:Heptane:MeOH (60:40:2 vlv/v) at a flow rate of 30--50 mUmin. with nitrogen gas. After sample application, 2.4 L--2.8 L of mobile phase was collected and discarded.
The mobile phase was then adjusted and fractions collected based on diol column bed volume. Fractions containing new components were then purified with semi-preparative high pressure liquid chromatography on PVA-Sil (polyvinyl alcohol functionalized silica gel), with peak collection based on UV signal monitoring at 265 nm.
For individual component purifications, minor mobile phase adjustments were made (see Scheme 1 ).
Five grams of enriched tail cut (ID 33285-i 04-1 ) were dissolved in 20 mL of CHpCI2:MeOH (96:4 v/v) and applied to 200 g (-. 400 mL) of pre-conditioned diol-bonded silica (40-63 p, irregular media) contained in a glass column (600 x 50 mm, 1.18 L). The diol column conditioning step prior to sample application involved the passage of 1 L of MeOH followed by 1.2 L of inifial mobile phase CH2CI2:Heptane:MeOH
(60:40:2 v/v/v). After sample application, 2.4 L of mobile phase was collected after which time the ratio of mobite phase was adjusted to 75:25:2 and maintained for an additional 8 L. lndividua! fractions were obtained and evaluated for the presence of minor components by analytical HPLC under the following isocratic conditions: PVA-Sil,.S ~ , 15 cm x 4.6 mm CH2C12:MeOH (98:2) l20 minutes. Complex 1 yielded 200 mg of material containing 15°~6 of a new component, Compound A, that is less polar than _7-antibiotic, 13-384, component 1. Complex 1 was further purified by dissolving 30--40 mg of the sample in 0.5 ml CH2CI2:MeOH (96:4 v/v) and injecting on a semi-preparative PVA-Sil column (250 x 20 mm) equilibrated with CH2CI2:MeOH (97.5:2.5 vlv). A flow rate of 12 mUmin yielded 5 mg of the desired material within a 12-14.2 minute elution time window. After four additional injections were made, a total of 24 mg of Compound A (>98%
pure) was obtained.
Complex 2 {100 mg) was further purified by using the same semi-preparative HPLC conditions described as above except a CH2Ci2:Heptane:MeOH (78:20:2, v/v/v) solvent system was used as the mobile phase. Two pure components, Compound E {1.5 mg) and Compound F {9.5 mg) were obtained. However, the first component (2.4 mg) was identified as a mixture of two compounds, Compound C and Compound D, based on analysis of spectroscopic data.
xample #2 Five grams of enriched head cut (ID 33285-104-2) was dissolved in 25 mL of CH2C12:MeOH (96:4 v/v) and applied to a recycled 200 g diol-bonded silica gel column. Recycling involved passing 1.5 L of MeOH followed by 1.5 L of starting mobile phase CH2CI2:Heptane:MeOH
(60:40:2 v/v/v). After sample application, 2.8 L of mobile phase was collected and effluent discarded. Mobile phase was adjusted to 75:25:2 (v/v/v) and 400 mL fractions were collected. Based on analytical HPLC, four fractions were pooled as complex 3, and yielded 360 mg of a yellowish powder after rotary evaporation. (The rest of the fractions were pooled as complex 4.) HPLC analysis of complex 3 on PVA-Sil indicated two peaks with elution times earlier than Compound A. Optimization studies for preparative chromatography led to the selection of a binary solvent system composed of n-butylchloride:MeOH which revealed the presence of a third entity. Approximately, 40-45 mg of complex 3 was then dissolved in 1.0 mL of CHZCI2:MeOH {96:4 vlv) and injected on a semi-preparative PVA Sil column {250 x 20 mm ) equilibrated with n-butylchloride:MeOH (93:7 v/v ). Flow rate was 15 mUminutes and UV
detection was at 265 nm. Three components were collected. The first two .B.
components (9.5 mg and 11.5 mg) were unstable and could not be identified. Only the third component (39 mg) was identified as Compound 8.
Complex 4 (6t mg) was further purified by a modified semi-preparative NPLC method similar to the conditions described above but with a different mobile phase of CH2CI2:MeOH (97.52.5, v/v ). Three pure components, Compound G (6.1 mg), Compound H {8.7 mg) and Compound J (27.5 mg) were obtained from this complex.

~9-, SCHEME 1 Tai) Cut ( ID 33285-104-1 ) Dioi-bonded silica (CH2CI2:Hept.:MeOH) (fi0:40:2) Step Gradient (75:25:2) Complex 1 Complex 2 PVA-Sil PVA-Sil (CH2CI2:MeOH) (CH2CI2:Hept:MeOH) (97.5:2.5) (78:20:2) Compound A
Mixture Compound E Compound F
(Compound C + Compound D) SCHEME 1. ctd.
Front Cut ( !D 33285-104-2) Diol-bonded silica (CH2CI2:Hept.:MeOH) (60:40:2) (Step Gradient) (75:25:2) (90:10:3) Complex 3 Complex 4 PVA-S!i PVA-S!i (n-BuCt:MeOH) (CH2Ci2:MeOH) (93:7) (97.5:2.5) Compound B
Compound G Compound H Compound J
Alt compounds were obtained as white powders after removal of solvents. The compounds are soluble in methanol, dimethyi sulfoxide, ethyl acetate, ac8tone and chloroform; partially sofubte in diethyl ether, dichloromethane end 1-chtorobutane; insoluble in hexane, , petroleum ether and water. The physico-chemical properties and spectroscopic data ofi these compounds of the invention are summarized in Table 1.

_ 11 _ LU ~ 1~ tti N
r r r N
o Q
N r_ CD tn N Cr7 ~!
c~? N
N N t'~~ N trp r U ~ coo r r Q
M
C
r r !. r N
N 111 C~r3 N
O O ~ a tte~7~ trD
U c'~ crc ~ r ~ e~
U ~ ~ M o r r r r N N ~ N
N r r r O
V ~ c~G ~
r e- r . N t~ O
r 0 r !~ r r .d. NN~"~ N~N
r r U
~' .' c~ cNO c°~o T r r a ~ ~ r r r r r N N N N r U ~ ~ '~ 'g r r D r Z ~ ~ T

_ 12 _ u1 + U
yn E
~.. ~ N
O

r i ~ r r r N
_CG ~ U N C'3 Z v~
~ LL
C
a_o O _ r - r U

N

+ V
'O~ 'o ~ Z a~ C'3 ,~ ~ O

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~
T
T

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N

m ,~ m E
Z ch N
r O a N O tL
= m r U

t ~ U

O
'~ ~ Z c~ O
r r ~t o~ ~L
O O
W
T
~
r .~ U

H

_~ ~ ~ 2 o m Q o ~ ~
a =
ti o D U
u~ 'r.~' ~ U
u.

- i3 -U
O r N +
r. C ~ c~CO CrC r._O O O
n r'r O cp =
C ~ N N C~ ~ cVcr! Z
~ r p N

U U
~ M N

.- i c p ~ cN0o z ~ ~ ~ ~ .+~ v U O ~ c'~co r T r o ~ ,~ ~ c~ui O
N N M c0 Z
O ~ ~ N r c'~ ~
-a U ~

r r r U c'~ ~ ~ + U

'a O ~ ~ r r p O C! N ~ ~ r .rt N N M ~ cN0 Z
U N r r O r et cvier r crc U
c~~

r r 'c"~ o N ~ c~ = C7 O r ~Q'tt) r r r r 0 ~- CC r O Z
~ N N ~ ~ N N
U G) T r O U
N r T ~

t' c~c~ c~N U
r r r T

m t' v v ~ m v ~
d W~ 97/13777 PCT/US96/15750 _ 1 q. _ .-. 'p m o C~ 'g v r ~ .m a s .~ ~ ~ .... c~ vi 0 r ~ ~ w Q
N r r r ~ ~ ~ .
~ O t1 O
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ca v~ o U ..-o 0 o_i ~ ~ N ~ o U ~ .- ~ _ca c E u' o .~ Q .S2 c E
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r V T r c c r~
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Z
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r 0 1 ~ ~ 1 S O

STRUCTURE ETERMINATION OF THE COMPOUNDS OF
3rHE INVENTION
The structures of the compounds were elucidated based on spectroscopic data analyses, including ultraviolet (UV), infrared (IR), Fast Atom Bombardment mass spectrometry (FAB-MS), proton and carbon-13 nuclear magnetic resonance (~H and ~3C NMR) methods. These compounds were characterized as novel eveminomicin related antibiotics.
'~3C NMR data of two important ortho-esters are listed in Table 1. ~H NMR
spectral data of individual compounds are shown in FIGS 1-8, respectively.
Assignments of some important protons and carbons were accomplished by attached proton test (APT), 2-dimensional nuclear Overhauser effect spectroscopy (NOESI~, heteronuclear multiple bond correlation (HMBC) and heteronuclear multiple quantum coherence (HMQC) experiments, as wail as by a direct comparison of spectral data with the antibiotic (13-384-component-1, eveminomicin) claimed in U.S.P. 4,597,968 as a reference standard.

The structure elucidation of Compound A was accomplished by analysis of mass and NMR spectroscopic data. The FAB mass spectral data showed a 34 amu increase of molecular weight by comparison with the reference sample (13-384-component-1, everninomicin). A trichloro-containing molecular ion cluster was observed in the FAB mass spectrum.
Both observations revealed the presence of a third chlorine atom in Compound A. The attachment of this extra chlorine atom to the aromatic ester fragment on right side of the molecule was determined based on a secondary fragmentation analysis in comparison with the reference sample (13-384-component-1, everninomicin). {DIAGRAM 1). However, the mass spectral data was unable to locate the exact position of the chlorine atom on the aromatic ring. The position of this chlorine at C-58 was further determined on the basis of NMR spectral data, indicating a -'IS-strong correlation of the proton-60 and methyl protons-82 in NOESY
experiments, and a connectivity between the proton-fi0 and the methyl carbon-fit in HMi3C experiments. Therefore, the structure of Compound A
was determined to be that shown in DIAGRAM 2. By utilizing the same methodology, stnJCtur85 Of other compounds were also elucidated and are illustrated in DIAGRAM 2. Compound C and H are shown in DIAGRAM 3. It should be noted that Compound J was characterized as a relatively small disaccharide linked to a bicyclic aromatic ester moiety through an ortho-ester functionality as shown DIAGRAM 4 below.

G
---;
:
O .-.
. ~..
:
/ 'Ov ..
O
O
.. bb.
O
V '0 0 g O s~ ~~
T 0 !~
O H ~t tlf b ~~ fix.
s ~. T
v a O O U

~~ -O-U
O ~ =o~ ~ ~ U

-Me Me Me ' O O O O O
Me A ~ 8 ~ off C
Me NOz i ~ Me CO
M ~ l R~ O H
R~ ~ 'CI
OH HO ' ' O
Me Mei OH
Rt R2 R3.R3. R4 R5 R8 evemino micin* - H OH OH,H OMe CI Me Compel A CI OH OH,H OMe CI Me Compel B H OH =O OMe CI Me Compel D H OMe flH,H OMe CI Me .

Compel E H OH OH,H OH H Me Compel F H OH OH,H OMe H Me Compel G H OH OH,H OMe CI H

*Eveminomicin 1'3-384 as is the antibiotic component disclosed 1 in USP 4,597,968 , , _ 19_ The italicized, capital letters identify the rings in the compounds of the invention The structures for compounds C and H are shown just below.

ll COmpOUnd ~'r ~T-~'r~g; ~g =C~"l3.
L'OmpOUnd ~ RT-Cr~~,H; Rg=~"~.
Tha structure for compound ,9 is shown just t; slow in Diagram 4.

~21-off ~o J
CH20Me O
~t~AeO
HD

BIOLOGICAL PROPERTIES OF THE COMPOUNDS OF THE INVENTION
The minor components were tested for activity based on an agar disk-diffusion protocol. Each component was dissolved at 1 mg/mL in CH2CI2 : MeOH (95:5 v/v) and a ten fold dilution made in the same vehicle.
Twenty microliters of each concentration was transferred to an B mm standard paper disk and allowed to air dry for thirty minutes. Each set of discs were placed on agar seeded with ~nhvlococcus aureus at two pH's (7/8) and incubated overnight at 35oC. Zones of sizes of inhibition are given below as the diameter of the circle of inhibition and are given in millimeters. The results are tabulated below:
Amount 20 pg 2 ttg 20 ~.g 2 p.g Everninomicin21 19 26 23 Corn ound 20 20 26 23 A

Corn ound 14 12 17 13 B

Corn ound 20 16 22 17 C/D

Corn ound 18 16 22 i 7 E

Corn ound 17 16 20 17 F

Corn ound NT NT NT NT
G

Corn ound NT NT NT NT
H

Corn ound 0 0 10 0 J

As used herein, NT means not tested.
The nearly equivalent potency of Compound A with everninomicin was further documented on a four fold dilution.
~ y'p~ antibiotic activity of the compounds of the invention can be demonstrated in mice via subcutaneous administration.
This invention may be carried out using pharmaceutically acceptable compositions comprising a pharmaceutically acceptable carrier and one or more compc,unds selected from the group consisting of A, B, C, D, E,1=, G, H and J.

As such, the antibiotics may be administered with any suitable pharma-ceutical carrier and administered orally, parenterally or topically in a variety of formulations. For oral administration, the antibiotics of this invention may be compounded in the form of tablets capsules, elixirs and the like. Tablets and capsules may contain such excipients as starch or lactose; liquid forms may contain coloring or flavoring agents. Topical preparations may be in the form of creams, hydrophobic or hydrophilic ointments or aqueous, non-aqueous emulsion-type lotions. Typical carriers for such formulations are water, oils, greases, polyesters and polyols. Parenteral formulations, e.g. injectible dosage forms are usually liquids such as solutions or suspensions with typical carriers being distilled water or saline solution.
The dose to be administered in any particular dosage form will depend on various factors, such as the characteristics of the animal species being treated, the susceptibility of the infecting organism to the antibiotic, and the stage and severity of the infection. Generally, the dosage administered is from about 1.0 mg to about 25 mg/kg of body weight per day, in divided dosages, the specified dosage being left to the discretion of the practitioner.
In treating certain patients with the compounds of this invention, it is possible to include other pharmaceutically active ingredients in the dosage unit.
THE MICROORGANISM
The microorganism used to obtain the compounds of this invention is a mutant strain of Micromonospora Carbonacea varAfricana as set forth in US
Patent 4,597,968. The way in which this mutant strain is obtained is as set forth in this application.
The mutant strain of Micromonospora Carbonacea varAfricana was prepared as set forth just below. Initially, parent strain SCC 1413 was subject to N-nitrosoguanidine (NTG) mutagenesis resulting in greater than a 90% kill of the culture. Fifteen hundred surviving isolates were examined for enhanced biological activity against S. aureus and E. coli. Single colony isolates were germinated in test tubes containing 10 mL of germination media and shaken at 250 r.p.m. on a gyratory shaker at 30flC
for 48 hours. Fermentation studies were initiated by transferring 2.5 mL of the seed to 250 mL Erlenmeyer flasks containing 50 mL of fermentation media and incubating at 30~C for 96 hours at 250 r.p.m. on a gyratory shaker. SCC 1631 was identified as an improved producer of the 13-384 complex on the basis of its improved bioactivity against ; , au~eus and .E.,.
Strain SCC 1756 was isolated by NTG mutation of SCG1631 followed uy selection of the isolates on agar plates containing 150 ~.g/mL
of eveminomicin(complex of vitro and nitroso analogs). Strain SCC 2146 was obtained by NTG mutagenesis of SCC 1756. Except for isolating the NTG mutagenized strains of SCC 1631 on the high levels of eveminomicin (complex of vitro and nitroso analogs), the protocols for both mutation studies were as previously described. For the latter two mutation studies, fermentation broths were extracted with ethyl acetate and the concentrates were chromatographed on Whatman LKG~F thin layer plates in a solvent system consisting of chioroform:methanol (9:i) followed by bioautography against $. aureus anii ~ ~ to confirm the production of ail components of the antibiotic complex. To follow the increased titres of eveminomicin (complex of vitro and nitroso analogs), thin layer plates were examined by using the Shimadzu CS-930 TLC plate scanner and quantitating the higher producing extracts using HPLC. Combined titers are defined as the sum of eveminomicin vitro and nitroso analogs.

Claims (20)

-25-

1. A compound of the formula:
selected from the group consisting of Compound A, B, D, E, F and G, as defined hereinafter, or a pharmaceutically acceptable salt thereof:
R1 R2 R3,R3, R4 R5 R6 Compound A Cl OH OH,H OMe Cl Me Compound B H OH =O OMe Cl Me Compound D H OMe OH,H OMe Cl Me Compound E H OH OH,H OH H Me Compound F H OH OH,H OMe H Me Compound G H OH OH,H OMe Cl H.

2. Compound A according to claim 1, wherein R1 is Cl, R2 is OH, R3 is OH, R3' is H, R4 is OMe, R5 is Cl and R6 is Me; or a pharmaceutically acceptable salt thereof.

3. Compound B according to claim 1, wherein R1 is H, R2 is OH, R3 and R3' taken together are =O, R4 is OMe, R5 is Cl and R6 is Me; or a pharmaceutically acceptable salt thereof.

4. Compound D according to claim 1, wherein R1 is H, R2 is OMe, R3 is OH, R3' is H, R4 is OMe, R5 is Cl and R6 is Me; or a pharmaceutically acceptable salt thereof.

5. Compound E according to claim 1, wherein R1 is H, R2 is OH, R3 is OH, R3' is H, R4 is OH, R5 is H and R6 is Me; or a pharmaceutically acceptable salt thereof.

6. Compound F according to claim 1, wherein R1 is H, R2 is OH, R3 is OH, R3' is H, R4 is OMe, R5 is H and R6 is Me; or a pharmaceutically acceptable salt thereof.

7. The Compound G according to claim 1, wherein R1 is H, R2, is OH, R3 is OH, R3' is H, R4 is OMe, R5 is Cl and R6 is H; or a pharmaceutically acceptable salt thereof.

8. A pharmaceutically acceptable salt of a compound i of claim 1.

9. A compound of the formula wherein R7=CH3; R8 =CH3; (Compound C) or R7=C(O)H; R8 =H (Compound H).
or a pharmaceutically acceptable salt thereof.

10. The Compound C according to claim 9, wherein R7 is CH3, and R8 is CH3;
or a pharmaceutically acceptable salt thereof.

11. The Compound H according to claim 9, wherein R7 is C(O)H and R8 is H;
or a pharmaceutically acceptable salt thereof.

12. The compound of the formula or a pharmaceutically acceptable salt thereof.

13. A composition comprising a compound of formula i according to any one of claims 1 to 7, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier material.

14. A composition comprising a compound of formula ii according to claim 9, or 11, or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier material.

15. Use of a compound of formula i according to any one of claims 1 to 7, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating bacterial infections in a mammal.

16. Use of a compound of formula ii according to claim 9, 10 or 11, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating bacterial infections in a mammal.

17. An antibacterial pharmaceutical composition comprising an acceptable, antibacterially effective amount of a compound of formula i as defined in any one of claims 1 to 7, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutically acceptable carrier.

18. An antibacterial pharmaceutical composition comprising an acceptable, antibacterially effective amount of a compound of formula ii as defined in claim 9, or 11, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutically acceptable carrier.

19. An antibacterial pharmaceutical composition comprising an acceptable, antibacterially effective amount of a compound of formula J as defined in claim 12, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutically acceptable carrier.

20. Use of a compound of formula J according to claim 12, or a pharma-ceutically acceptable salt thereof, in the manufacture of a medicament for treating bacterial infections in a mammal.