CA2229540A1 - Systeme de marquage par epitopes - Google Patents

Systeme de marquage par epitopes Download PDF

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Publication number
CA2229540A1
CA2229540A1 CA002229540A CA2229540A CA2229540A1 CA 2229540 A1 CA2229540 A1 CA 2229540A1 CA 002229540 A CA002229540 A CA 002229540A CA 2229540 A CA2229540 A CA 2229540A CA 2229540 A1 CA2229540 A1 CA 2229540A1
Authority
CA
Canada
Prior art keywords
protein
tagged
hexapeptide
epitope
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002229540A
Other languages
English (en)
Inventor
Linfa Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2229540A1 publication Critical patent/CA2229540A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention porte sur des hexapeptides ayant la fonction d'épitopes marqueurs lorsqu'ils se fixent à des molécules considérées. Des marqueurs de ce type, tels que ceux présentant la séquence Q-Y,F,H ou W-P-A,S ou V-L ou V-T,L, V ou Q, ou mieux QYPALT, QYPSLL, QYPSLQ, QFPALL, QYPVLV et QYPSLT ou idéalement QYPALT, servent à la purification d'une protéine considérée, ou, à l'étude ou à la caractérisation d'une molécule considérée.
CA002229540A 1995-08-15 1996-08-15 Systeme de marquage par epitopes Abandoned CA2229540A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPN4800A AUPN480095A0 (en) 1995-08-15 1995-08-15 Epitope tagging system
AUPN4800 1995-08-15

Publications (1)

Publication Number Publication Date
CA2229540A1 true CA2229540A1 (fr) 1997-02-27

Family

ID=3789131

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002229540A Abandoned CA2229540A1 (fr) 1995-08-15 1996-08-15 Systeme de marquage par epitopes

Country Status (5)

Country Link
EP (1) EP0845004A4 (fr)
JP (1) JPH11510683A (fr)
AU (1) AUPN480095A0 (fr)
CA (1) CA2229540A1 (fr)
WO (1) WO1997007132A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19733389C2 (de) * 1997-08-01 1999-08-19 Deutsches Krebsforsch Mittel zur Expression und zum Nachweis eines ein HPOL-Epitop und ein Polypeptid enthaltenden Fusionspolypeptids
DE19852800C1 (de) * 1998-11-16 2000-04-13 Univ Albert Ludwigs Freiburg Verfahren zur Herstellung von Antikörpern gegen ein Polypeptid, von dem die kodierende Nukleinsäure bekannt ist
DE10054055A1 (de) 2000-10-31 2002-05-23 Nmi Univ Tuebingen Verfahren zur Analyse von Proteinen
US7460960B2 (en) 2002-05-10 2008-12-02 Epitome Biosystems, Inc. Proteome epitope tags and methods of use thereof in protein modification analysis
US7618788B2 (en) 2002-05-10 2009-11-17 Millipore Corporation Proteome epitope tags and methods of use thereof in protein modification analysis
GB0217402D0 (en) * 2002-07-26 2002-09-04 Roslin Inst Edinburgh Multi-reporter gene model for toxicological screening
FR2857374B1 (fr) * 2003-07-08 2005-09-23 Centre Nat Rech Scient Glutamine:fructose-6-phosphate amidotransferase(gfat) comprenant une etiquette de purification interne, et son utilisation pour le criblage de composes
US7855057B2 (en) 2006-03-23 2010-12-21 Millipore Corporation Protein splice variant/isoform discrimination and quantitative measurements thereof
WO2013091661A2 (fr) * 2011-12-23 2013-06-27 Aarhus Universitet Etiquette d'affinité protéique résistante à la protéolyse
PT3532612T (pt) * 2016-10-31 2022-07-26 Univ Zuerich Coleta de marcadores e métodos para deteção de proteínas, de preferência por espectroscopia de massa

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4237113B4 (de) * 1992-11-03 2006-10-12 "Iba Gmbh" Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins
US5652128A (en) * 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins

Also Published As

Publication number Publication date
WO1997007132A1 (fr) 1997-02-27
EP0845004A4 (fr) 1999-06-30
AUPN480095A0 (en) 1995-09-07
EP0845004A1 (fr) 1998-06-03
JPH11510683A (ja) 1999-09-21

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued