CA2222041A1 - 1-benzoyl-2-(indolyl-3-alkyl)-piperazine derivatives as neurokinin receptor antagonists - Google Patents

Abstract

This invention relates to compounds of generic formula (I), to a process for preparation thereof, to a pharmaceutical composition comprising the same, and to a use of the same as a medicament. In formula (I), R1 is trihalo(lower)alkyl, R2 is trihalo(lower)alkyl, R3 is indolyl(lower)alkyl, -A- is -CH2- or (a), and -R4 is (b), (c) or (d) in which R5 is hydrogen or lower alkoxycarbonyl, R6 is hydrogen or lower alkanoyl, R7 is hydrogen, lower alkyl, lower alkanoyl, lower alkoxycarbonyl, lower alkoxy(lower)alkanoyl, cyclo(lower)alkylcarbonyl, aroyl or lower alkylsulfonyl, or its pharmaceutically acceptable salt.

Description

D E S C R I P T I O N
1 3ENZOYL-2-(INDOLYL-3-ALltYL)-PIPERAZINE DERIVATIVES AS NEUROKININ RECEPTOR
ANTAGONISTS
.

TECED~IC~iL FIELD
The preser.t invention relates to new piperazine derivatives and a pharmaceutically acceptable salt thereof.
More particularly, it relates to new piperazine derlvatives and a pharmaceutically acceptable salt thereof which have pharmacological activities such as Tachykinin antagonism, especially Substance P antagonism, Neurokinin A
antagonism, Neurokinin B antagonism, and the like, to a process for preparation thereof, to a pharmaceutical composition comprisi~g the same, and to a use of the same as a medicament.
Accordingly, one object of the present invention is to provide new and useful piperazine derivatives and a pharmaceutically acceptable salt thereof which have pharmacological activities such as Tachykinin antagonism, especially Substance P antagonism, Neurokinin A antagonism, Neurokinin B antagonism, and the like.
Another object of the present invention is to provide a process for the preparation of said piperazine derivatives and a salt thereof.
A further object of the present invention is to provide a pharmaceutical composition comprising, as an active ingredient, said piperazine derivatives and a pharmaceutically acceptable salt thereof.
Still further object of the present invention is to provide a use of said piperazine derivatives or a pharmaceutically acceptable salt thereof as Tachykinin antagonist, especially Substance P antagonist, Neurokinin A
antagonist or Neurokinin B antagonist, useful for treating or preventing Tachykinin-mediated diseases, for example, respiratory diseases such as asthma, bronchitis, rhinitis, cough, expectoration, and the like; ophthalmic diseases such as conjunctivitis, vernal conjunctivitis, and the likei cutaneous diseases such as contact dermatitis, atopic dermatitis, urticaria, and other eczematoid dermatitis, and the like; inflammatory diseases such as rheumatoid arthritis, osteoarthritis, and the likei pains or aches (e.g., migraine, headache, toothache cancerous pain, back pain, etc.); and the like in human being or animals.

DISCLOSURE OF INVENTION
The object compound of the present invention is the compound of the following formula (I):

CF3 ~

F3C ~ C ~ 'N~ ~ (I), namely (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-4-[N-(4-methyl-1-piperazinyl)carbamoylmethyl]-piperazine, or fumaric acid salt thereof, namely (2R)-1-[3,5-bis(trifluoromethyl)benzoylJ-2-(lH-indol-3-ylmethyl)-4-[N-(4-methyl-1-piperazinyl)carbamoylmethyl]piperazine fumarate [hereinafter referred to briefly as compound (If)].

The other object compound of the present invention can be represented by the following general formula (Ig) :

R2 C ~ ~ -A-R4 (Ig~

wherein R1 is trihalo(lower)alkyl, R2 is trihalo(lower)alkyl, R3 is indolyl(lower)alkyl, -A- is -CH2- or -~-CH2- , and -R4 is ~ ~ ~R7 ~ ~R7 or ~s~ ~R7 in which R5 is hydrogen or lower alkoxycarbonyl, R6 is hydrogen or lower alkanoyl, R7 is hydrogen, lower alkyl, lower alkanoyl, lower alkoxycarbonyl, lower alkoxy(lower)alkanoyl, cyclo(lower)alkylcarbonyl, aroyl or lower alkylsulfonyl, or its pharmaceutically acceptable salt.

According to the present invention, the object compounds can be prepared by processes which are illustrated in the 35 following schemes.

Process 1 H2N-N ~CH3 ~N~_" ~ (III) 11 11 or its reactive CF3 ~ derivative at the ~ -. amino group f ~ A ~ or a salt thereof F3C ~ ~N N~_~C~

(II) or its reactive derivative at the carboxy group or a -~alt thereof F3C ~ ICl ~ C~ ~N

(I) or a salt thereof Process 2 CF3 ~ ~O2CCCH
~ 1~l ~ NCH3 H CO2H
F3C ~ C ~N ~ C~N~N J

(I) or a salt thereof 1 other than fumaric acid ' 35 salt thereof C ~ I~ ~CH3 H~COzH

(If) Process 3 l5Rl W-Al-R4 R2 ~ ~ ~ -H thereof ~ ~

(IV) (Ig') or its reactive derivative or a ~alt thereof at the imino group or a salt thereof Process 4 HOOC-Al-R4 (VI) l or it~ reactive Rl 3 R \ R3 derivative at the ~ \ O
~ ~ carboxy group ~ C~N\~ -H or a salt thereof ~ c~N\__~N-C-Al-R4 (IV) (Ig") or its reactive derivative or a ~alt thereof at the imino group or a Yalt thereof wherein Rl, R2, R3 and R4 are each as defined above;
-A1- is -CH2-; and W is a leaving group.

Suitable salts and pharmaceutically acceptable salts of the starting and object compounds are conventional non-toxic salt and include an acid addition salt such as an organic acid salt (e.g. acetate, trifluoroacetate, fumarate, maleate, tartrate, methanesulfonate, benzenesulfonate, formate, toluenesulfonate, etc.), an inorganic acid salt (e.g.
hydrochloride, hydrobromide, hydroiodide, sulfate, nitrate, phosphate, etc.), or a salt with an amino acid (e.g.
arginine, aspartic acid, glutamic acid, etc.), or a metal salt such as an alkali metal salt (e.g. sodium salt, potassium salt, etc.) and an alkaline earth metal salt (e.g.
calcium salt, magnesium salt, etc.), an ammonium salt, an organic base salt (e.g. trimethylamine salt, triethylamine salt, pyridine salt, picoline salt, dicyclohexylamine salt, N,N'-dibenzylethylenediamine salt, etc.), or the like.
In the above and subsequent descriptions of the present specification, suitable examples and illustrations of the various definitions which the present invention include within the scope thereof are explained in detail as follows.
The term "lower" is intended to mean 1 to 6, preferably 1 to 4 carbon atom(s), unless otherwise indicated.
Suitable "lower alkyl" and "lower alkyl moiety" in the terms "indolyl(lower)alkyl" and "lower alkylsulfonyl" is straight or branched one having 1 to 6 carbon atom(s) and may include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, pentyl, hexyl and the like.
Suitable "trihalo(lower)alkyl" may include trichloromethyl, tribromomethyl, trifluoromethyl and the like.
Suitable "lower alkoxy" and "lower alkoxy moiety" in the terms "lower alkoxycarbonyl" and q'lower alkoxy(lower)alkanoyl" may include methoxy, ethoxy, isopropyloxy, butoxy and the like.
Suitable "lower alkanoyl" and "lower alkanoyl moiety" in the term "lower alkoxy(lower)alkanoyl" may include formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, hexanoyl, pivaloyl and the like.
Suitable "cyclo(lower)alkyl moiety" in the term "cyclo(lower)alkylcarbonyl" may i~clude cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and the like.
Suitable "aroyl" may include benzoyl, toluoyl, naphthoyl and the like.
Suitable "leaving group" may include hydroxy, reactive group derived from hydroxy and the like.
Suitable "reactive group derived from hydroxy" may include acid residue and the like.
Suitable "acid residue" may include halogen (e.g.
fluoro, chloro, bromo, iodo), acyloxy (e.g. acetoxy, tosyloxy, mesyloxy, etc.) and the like.
The Processes 1 to 4 for preparing the object compounds of the present invention are explained in detail in the following.

= 25 Process 1 The object compound (I) or a salt thereof can be prepared by reacting a compound (II) or its reactive derivative at the carboxy group or a salt thereof with a compound (III) or its reactive derivative at the amino group or a salt thereof.
Suitable reactive derivative at the carboxy group of the compound (II) may include an acid halide, an acid anhydride, an activated amide, an activated ester, and the like.

_ Suitable examples of the reactive derivatives may be an acid chloride; an acid azide; a mixed acid anhydride with an acid such as substituted phosphoric acid [e.g.
dialkylphosphoric acid, phenylphosphoric acid, diphenylphosphoric acid, dibenzylphosphoric acid, halogenated phosphoric acid, etc.], dialkylphosphorous acid, sulfurous acid, thiosulfuric acid, sulfuric acid, sulfonic acid [e.g.
methanesulfonic acid, etc.], aliphatic carboxylic acid [e.g.
acetic acid, propionic acid, butyric acid, isobutyric acid, pivalic acid, pentanoic acid, isopentanoic acid, 2-ethylbutyric acid, trichloroacetic acid, etc.] or aromatic carboxylic acid [e.g. benzoic acid, etc.]i a symmetrical acid anhydridei an activated amide wlth imidazole, 4-substituted imidazole, dimethylpyrazole, triazole or tetrazolei or an activated ester [e.g. cyanomethyl ester, methoxymethyl ester, dimethyliminomethyl [(CH3)2N=CH-] ester, vinyl ester, propargyl ester, p-nitrophenyl ester, 2,4-dinitrophenyl ester, trlchlorophenyl ester, pentachlorophenyl ester, mesylphenyl ester, phenylazophenyl ester, phenyl thioester, p-nitrophenyl thioester, p-cresyl thioester, carboxymethyl thioester, pyranyl ester, pyridyl ester, piperidyl ester, 8-quinolyl thioester, etc.], or an ester with a N-hydroxy compound [e.g. N,N-dimethylhydroxylamine, 1-hydroxy-2-(lH)-pyridone, N-hydroxysuccinimide, N-hydroxyphthalimide, 1-hydroxy-lH-benzotriazole, etc.], and the like. These reactive derivatives can optionally be selected from the above according to the kind of the compound (II) to be used.
Suitable reactive derivative at the amino group of the compound (III) may include Schiff's base type imino or its tautomeric ~n~mine type isomer formed by the reaction of the compound (III) with a carbonyl compound such as aldehyde, ketone or the like; a silyl derivative formed by the reaction of the compound (III) with a silyl compound such as bis(trimethylsilyl)acetamide, mono(trimethylsiLyl)acetamide, bis(trimethylsilyl)urea or the like; a derivative formed by W 096t37489 PCT/JP9610133S

reaction of the compound (I~-I) with phosphorus trichloride or phosgene and the like.
~ The reactlon is usually carried out in a conventional solvent such as water, alcohol [e.g. methanol, ethanol, etc.], acetone, 2-butanone, dioxane, acetonitrile, chloroform, methylene chloride, ethylene chloride, tetrchydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine or any o~her org2nic sol~ent which does not adversely influence the reaction, or the mixture thereof.
In this reaction, when the compound (II) is used in a free acid form or its salt form, the reaction is preferably carried OUI in the presence of a conventional condensing agent such as N,NI-dicyclohexylcarbodiimide;
N-cyclohexyl-N'-morpholinoethylca~~bodiimide;
~-cyclohexyl-N'-(4-diethylaminocyclohexyl)carbodlimidei N,N'-diethylcarbodiimide; N,N'-diisopropylcarbodiimide;
N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide;
pentamethyleneketene-N-cyclohexyliminei diphenylketene-N-cyclohexyli~ine; ethoxyacetylene;
1-alkoxy-1-chloroethylene; trialkyl phosphite;
ethyl polyphosphate; isopropyl polyphosphate;
phosphorus oxychloride (phosphoryl chloride); phosphorus trlchloride; diphenyl phosphorylazide; thionyl chloride;
oxalyl chloride; lower alkyl haloformate [e.g. ethyl chloroformate, isopropyl chloroformate, etc.];
triphenylphosphine; 2-ethyl-7-hydroxybenzisoxazolium salt;
2-ethyl-5-(m-sulfopheryl)isoxazollum hydroxide intramolecular s~lt; 1-(p-chlorobenzenesulfonyloxy)-6-chloro-lH-benzotriazole; 1-hydroxybenzotriazole; so-called Mukaiyama reagent such as 2-chloro-1-methylpyridinium iodide;
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by itself or ir combination with 1-hydroxybenzotriazole;
so called Vilsmeier reagent prepared by the reaction of N,N-dimethylformamide witr. thionyl chloride, phosgene, trichloromethyl chloroformate, phosphorus oxychloride, etc.;

or the like, or the mixture thereof.
The reaction may also be carried out in the presence of an inorganic or organic base such as an alkali metal bicarbonate, tri(lower)alkylamine, pyridine, N-(lower)alkylmorpholine, N,N-di(lower)alkylbenzylamine, or the like.
The reaction temperature is not critical, and the reaction is usually carried out under cooling to warming.

Process ~
The object compound (If) can be prepared by reacting the compound (I) or a salt thereof other than fumaric acid salt thereof with fumaric acid.
The reaction is usually carried out in a conventional solvent such as water, alcohol [e.g. methanol, ethanol, etc.], acetone, 2-butanone, dioxane, acetonitrile, chloroform, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine or any other organic solvent which does not adversely influence the reaction, or the mixture thereof.
The reaction temperature is not critical, and the reaction is usually carried out under cooling to warming.

Process 3 The object compound (Ig') or a salt thereof can be prepared by reacting the compound (IV) or its reactive derivative at the imino group or a salt thereof with the compound (V) or a salt thereof.
Suitable reactive derivative at the imino group of the compound (IV) may include Schiff's base type imino or its tautomeric en~ine type isomer formed by the reaction of the compound (IV) with a carbonyl compound such as aldehyde, ketone or the like; a silyl derivative formed by the reaction of the compound ~IV) with a silyl compound such as bis(trimethylsilyl)acetamide, mono(trimethylsilyl)acetamide, .

bis(trimethylsilyl)urea or the like;
a derivative formed by reaction of the compound (IV) with phosphorus trichloride or phosgene and the like.
This reaction is usually carried out in a solvent such as alcohol [e.g. methanol, ethanol, etc.], dichloromethane, benzene, N,N-dimethylformamide, tetrahydrofuran, diethyl ether or any other solvent which does not adversely affect the reaction.
The raction may be carried out in the presence of an inorganic or an organic base such as an alkali metal hydroxide [e.g. sodium hydroxide, potassium hydroxide, etc.], an alkali metal carbonate [e.g. sodium carbonate, potassium carbonate, etc.], an alkali metal bicarbonate [e.g. sodium bicarbonate, potassium bicarbonate, etc.], alkali metal hydride [e.g. sodium hydride, potassium hydride, etc.], tri(lower)alkylamine [e.g. trimethylamine, triethylamine, diisopropylethylamine, etc.], pyridine or its derivative [e.g. picoline, lutidine, 4-dimethylaminopyridine, etc.], or the like. In case that the base to be used in liquid, it can also be used as a solvent.
The reaction temperature is not critical, and the reaction can be carried out under cooling, at room temperature or under warming or heating.

Process 4 The compound (Ig") or a salt thereof can be prepared by reacting a compound (IV) or its reactive derivative at the imino group or a salt thereof with a compound (VI) or its reactive derivative at the carboxy group or a salt thereof.
The reaction can be carried out in the manner disclosed in Preparation 10 or similar manners thereto.

The object compounds of the present invention have pharmacological activities such as Tachykinin antagonism, especially Substance P antagonism, Neurokinin A antagonism or Neurokinln B antagonism, and therefore are useful for treating or preventing Tachykinin-mediated diseases, particul2rly Substance P-med-ated diseases, for example, respiratorv diseases such as ast'~a, bronchitis (e.g. chronic bronchitis, acule bronchitis and diffuse panbronchiolitis, etc.~, rhinltis, cough, expectoratlon, and the like ophthalmic diseases such as conjunctivitis, vernal conjunctivltis, and the likei cutaneous dlseases such as conlacl dermatitis, atopic dermatitis, urticaria, and other eczematoid dermatitis, and the like; inflammatory diseases such as rheumatoid arthritis, osteoarthritis, and the like;
pains or aches (e.g. migraine, headache, cluster headache, ~oothache, cancerous pain, back pain, neuralgia, etc.); and the like.
Further, it is expected that the object compounds of the present invention are useful for treating or preventing ophthalmic diseases such as glauco~a, uveitis, and the like;
gastrointestlnal diseases such as ulcer, ulcerative colitis, irrltable bowel syndrome, food allergy, and the like;
lnflammatory diseases such as nephritis, and the like, circulatory diseases such as hypertension, angina pectoris, cardiac failure, thro~osis, Raynaud~s disease, and the like;
epilepsyi spastlc paralysisi pollakiurla; Cystilis; bladder detrusor hyperreflexia; urinary incontlnence; Parkinson diseasesi dementiai AIDS related dementiai Alzheimer's diseasesi Down's syndromei Huntington's chorea; carcinoid syndromei disorders related to immune enhancement or suppressioni disorders caused by Helicobacier pylori or another splr21 urease-positlve gram-negative bacteriumi sunburni angiogenesis or diseases caused by angiogenesis; and the like.
I L is furthermore expected that the object compounds of ~he present invention are useful for treating or preventing chronic obstructive pulmonary diseases, particularly chronic pulmonary emphysema; irltisi prollferative vitreoretinopathy;
psoriasls; nflam~atory intestinal diseases, particularly Crohnls diseasesi hepatiti si superficlzl pain on congelation, burn, herpes zoster or diabetic neuropathy; tenalgia attended to hyperlipldemiai postoperative neuroma, particularly of mastectomyi vulvar vestibulitis; hemodi21ysis-associated ~tchingi lichen plarusi laryngopharyngitis; bronchiectasis;
coniosisi whooping coughi pulmonary tuberculosisi cystic Sibrosis; e~esis; men.al diseases, particularly anxiety, depression, dysthymic disorders and schizophrenia;
demyelinating diseases such as multiple sclerosis and amyotrophic lateral sclerosisi attenuation of morphine wl~hdrawal; oedema, such as oedema caused by thermal injury;
small cell carclnomas, particularly small cell lung cancer (SCLC); hypersensitivity disorders such as poison ivy;
fibrosing and collagen diseases such as scleroderma and eosinophilic fascioliasisi reflex sympathetlc dystrophy such as shoulder/hand syrdrome; addiction disorders such as alcoholismi stress related somatic disordersi rheumatic diseases such as fibrositisi and the like.
For therapeutic purpose, the object compounds of the present invention can be used in a form of pharmaceutical preparation containing one or said compound, as an active ingredient, in admixture with a pharmaceutically acceptable carrier such as an organic or lnorganic solid or liquid excipient suitable for oral, parenteral, external including topical, enteral, intravenous, intramuscular, inhalant, nasal, ir.traarticular, intrasplnal, transtracheal or transocular administration. The pharmaceutical preparations 3C ~.ay be solid, se~l-solid or solut-ons such as capsules, tablets, pellets, dragees, powders, granules, suppositories, ointments, creams, lotions, inhalants, injections, cataplasms, gels, tapes, eye drops, solution, syrups, aerosols, suspension, e~ulsion, or the like. If desired, there may be included in these preparations, auxiliary - 14 - ~
substances, stabilizing agents, wetting or emulsify~ng agents, buffers and other commonly used zdditives.
While the dosage of the object compounds will vary depending upon the age and condition of a patient, an average single dose of about 0.1 mg, 1 mg, 10 mg, 50 mg, 100 mg, 250 mg, 500 mg and 1000 mg of the object compounds may be effectlve for treating Tachyk~nin-mediated diseases such as asthma and the like. In general, amounts between 0.1 mg/body and about 1,000 mg/bodv may be administered per day.
In order to illustrate the usefulness of the object compounds, the pharmacological test data of a representative compound o~ ine present invention is shown in the following The fcllowing Test Compound showed more than 90%
inhibition rate of 125I-BH-Substance P binding to h-NK
receptors at ~he concentration of 0.1 ~g/ml.

Test Compound : The object compound of the Example 2 125I-BH-Substance P Binding to h-NK1 Receptors Test Method : 125I-BH-Substance P Binding to h-NK1 Receptors (a) Crude CH0 cell membrane preparation CHO cells permanently expressing h-NK1 receptors were harvested and homogenized with a Dounce homogenizer at 4~C in z buffer (0.25 M suc~ose, 25 mM Tris-HC1 pH 7.4, 10 mM MgC12, 1 mM EDTA, 5 ~Ig/ml p-APMSF). The homogenate was centrifuged (500 x g, 10 minutes), and the pellet was resuspended in the same buffer, homogenized, and centrifuged. The two supernatants were combined and centrifuged (100,000 x g, 1 hour). The crude cell membranes thus isolated were resuspended in buffer (25 mM Tris-HCl pH 7.4, 10 mM MgC12, W 096t37489 PCT/JP96/01335 ~ 15 ~
1 ~ EDTA, 5 ~/ml p-APMSF) and stored at -80~C until use.

(b) 125I-BH-Substance P binding to preparation me~brane Cell membranes (6 ~g/ml) were incubated with 125I-BH-Substance P (0.1 nM) with or without test compounds in 0.25 ml of Medium. 2 (50 mM Tris-HCl pH 7.4, 5 ~Ll'I MnC12, 20 ~g/ml chymostatin, GO ,ug/ml bacitracin, 4 ug/ml leupeptin, 5 ,ug/ml p-APMSF, 200 ~g/ml BSA) at 22~C for gO minutes. At the end or the incubaiion period, the content was quickly filtered over a ~ahtman GF/C glass filter (pretreated with 0.1%
polyethylene ~mine for 3 hours prior to use) under aspiratlor. Each of the filters was then washed four times with 5 ml of buffer (50 mM Tris-~Cl p~ 7.4, 5 mM MnC12). The radioaclivity was counted by using Auto Gamma counter (Packerd RIASTAR 5420A). A11 d~ta presented are specific binding defined as that displaceable by 3 ~M unlabeled Substance P.

Further, the object compound of the present invention, especially the compound (If), is also superior in stability and the like.

~X~MPLES
The following Preparations and Examples are given for the purpose of illustrating the present invention in more detail.

- to be continued on the next page -Preparation 1 fHo O
tert~C 4 H9~~-C~NH~<~

1=0 To a mixture of N2-(tert-butoxycarbonyl)-N1-formyl-D-tryptophan (3.99 g) and N-benzyl glycin benzyl ester hydrochloride (3.50 g) in dichloromethane (70 ml) was added triethylamine (5.85 ml) under nitrogen atmosphere. To the mixture was added 2-chloro-1-methylpyridinium iodide (3.67 g) at room temperature, and the resulting mixture was stirred for 2 hours. After the reaction was completed, dichloromethane (30 ml) and water (30 ml) were added. The organic layer was separated, washed with 0.5N hydrochloric acid (10 ml), water (10 ml), aqueous sodium bicarbonate sclution (10 mlj and brine (20 ml) successively and dried over magnesium sulfate. After evaporation of the solvent, the residue was purified on a silica gel column (140 g) eluting with a mixture of toluene and ethyl acetate (4:1) to give (2R)-N-benzyl-N-benzyloxycarbonylmethyl-2-(tert-butoxycarbonylamino)-3-(N-formyl-lH-indol-3-yl)propanamide (6.41 g) as an oil.

IR (CHCl3) : 3300, 2970, 1740, 1700, 1644, 1604 cm~~
NMR ~DMSO-d6, o) : 0.89, 1.22 and 1.29 (9H, 3 s);
2.80-3.10 (2H, m); 3.95-4.25 (2H, m); 4.40-4.90 (3H, m); 4.95-5.20 (2H, m); 7.05-7.75 (15H, m);
7.98 and 8.22 (lH, 2 br s)i 9.2~ and 9.61 (lH, 2 br s ) MASS : 570 (M+1) Preparation fHo /~ ' HN ~

0~

To an ice-cooled solution of the object compound of Preparation 1 (6.39 g) in dichloromethane (50 ml) was added 4N hydrogen chloride ln dioxane solution (50 ml). The mixture was stirred al the same temperature for 30 minutes and at room temperature for 1 hour. After evaporation of the solvent, the residue was partitioned between dichloromethane (50 ml) and aqueous sodium bicarbonate solution (30 ml). The organic layer was separated, dried over magnesium sulfate and filtered. To the filtrate was added triethylamine (1.67 ml) at room temperature, and the mixture W25 stirred for 1.5 ~ hours. After evaporation, the residue was triturated with diisopropyl ether, collected by filtration and dried to give (3R)-l-benzyl-3-(N-formyl-lH-indol-3-ylmethyl)piperazine-2,5-dione (3.93 g).

mp : 176-178~C
IR (Nujol) : 3250, 1709, 1648, 1630 cm 1 NMR (DMSO-d6, o) : 2.95-3.30 and 3.35-3.70 (4H, 2 m);
4.22 (lH, d, J=14.6Hz); 4.30-4.40 (lH, m); 4.54 (lH, d, J=14.9Hz); 6.80-7.75 (9H, m); 7.95-8.50 (2H, m); 9.20 and 9.65 (lH, 2 br s) Prep~r~tio~ 3 H

To an ice-cooled solution of the object compound of Preparation 2 (3.89 g) in a mixture of methanol (175 ml) and tetrahydrofuran (50 ml) was added aqueous O.lN sodium hydroxide solution (108 ml). The mixture was stirred at the same temperature for 30 minutes and at room temperature for 1.5 hours. After evaporation of the solvent, the residue was extracted with dichloromethane. The organlc layer was washed with water and an aqueous sodium chloride solution, and dried over magnesium sulfate. Evaporation of the solvent gave (3R)-l-benzyl-3-(lH-indol-3-ylmethyl)piperazine-2,5-dione (3.68 g) mp : 207-208~C
IR (Nujol) : 3402, 1650 cm 1 NMR (DMSO-d6, o) : 2.68 (lH, d, J=17.2Hz); 3.04 (lH, dd, J=14.4 and 4.4Hz); 3.20-3.40 (2H, m); 4.24 (lH, s); 4.10-4.40 (2H, m); 6.75-7.60 (lOH, m); 8.35 (lH, s)i 10.94 (lH, s) MASS : 334 (M+1) Pre~aration 4 ,~
Y~/~

To a suspension of lithium aluminum hydride (0.77 g) in tetrahydrofuran (~0 ml) was added dropwise a solution of the object compound of Preparation 3 (3.40 g) in tetrahydrofuran (40 ml) at 0~C under nitrogen atmosphere. The mixture was stirred at room temperature for 50 minutes and at refluxing temperature for 1 hour. The resulting mixture was diluted with tetrahydrofuran (60 ml) and cooled to 0~C. Water (3.0 r.l) znd aqueous 15% sodium hydroxide solution (0.8 ml) were added slowly. The resulting insoluble inorganic material was removed by filtration and washed with tetrahydrofuran. The filtrate and the washing were combined and evaporated under reduced pressure to give (3R)-1-benzyl-3-(lH-indol-3-ylmethyl)piperazine (3.68 g) as an oil.
IR (CHCl3) : 3240, 3040, 290C cm 1 NMR (DMSO-d6, o) : 1.70-2.00 and 2.30-2.45 (2H, 2 m);
2.50-3.00 (7H, m); 3.25-3.6C (3H, m); 6.80-7.60 (lOH, m); 10.80 (lH, s) ~SS : 306 (M+1) . - 20 -Preparation 5 S o ~

CF3~C~N~

To a mixture of 3,5-bis(trifluoromethyl)benzoic acid (1.15 g) and (3R)-1-benzyl-3-(lH-indol-3-ylmethyl)piperazine (1.61 g) in dichloromethane (80 ml) was added triethylamine (1.55 ml) at room temperature under nltrogen atmosphere.
2-Chloro-1-methylpyridinium iodide (1.37 g) was added, and the mixture was stirred at room temperature for 2.5 hours.
The resulting mlxture was poured into water (20 ml). The organic layer was washed successively with 0.5N hydrochlorlc acid, water, aqueous sodium bicarbonate solution and brine, and drled over magneslum sulfate. After evaporation under reduced pressure, the residue was chromatographed on silica gel with toluene - ethyl acetate (4:1) as an eluent to give (2R)-4-benzyl-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (0.87 g) as a syrup.
TR (CHCl3) : 3430, 3300, 3000, 2910, 2800, 1630-1610 cm~l NMR (DMSO-d6, o) : 1.90-2.40 (2H, m); 2.70-3.90 (8H, m)i 4.25-4.40 and 4.75-4.90 (lH, m)i 6.50-7.45 (lOH, m); 7.50-8.25 (3H, m); 10.77 (lH, s) M~S : 546 (M+l) Pre~aration 6 j ~ J

Il CF3 ~ ~ N NH

A mixture of (2R)-4-benzyl-1 [3,5-bls(trifluoromethyl)-benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (5.20 g), ammonium formate (1.50 g) and 10~ Pd charcoal (0.52 g) in ethanol (50 ml) was refluxed for 7.5 hours under nitrogen atmosphere.
The reaction mixture was cooled to room temperature and filtered through Celite pad. The filtrate was concentrated under reduced pressure and the residue was purified on a silica gel column eluting with a mixture of dichloromethane and methanol (20:1) to give (2R)-1-[3,5-bis(trifluoromethyl)-benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (2.67 g) as a syrup.
IR (CHC13) : 3280, 2900, 1622 cm 1 NMR (DMSO-d6, o) : 2.50-3.50 (9H, m); 3.6-4.8 (lH, m);
6.55-7.40 (5H, m); 7.50-8.22 (3H, m); 10.84 (lH, s) MASS : 456 (M+l) Prep~r~tion 7 A mixture of (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (1.5 g), benzyl 2-bromoacetate (0.75 g), triethylamine (0.55 ml) and tetrahydrofuran (15 ml) was stirred overnight at room temperature. The resulting insoluble material was removed by filtration and the filtrate was concentrated under reduced _ pressure. The residue was pur fied by column chromatography on silica gel eluting with a mixture of dichloromethane and methanol (30:1) to give (2R)-4-(benzyloxycarbonylmethyl)-1-r3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-piperazine (1.92 g).
[a]D1 : -11.6~ (C=1.0, MeOH) IR (Neat) : 3600-3100, 1735, 1626, 1275, 1129, 900 cm 1 NMR (DMSO-d6, o) : 2.20-5.20 (13H, ~,); 6.60-8.20 (13H, m); 10.85 (lH, br s) MASS : 604 (M+1), 454 Preparation 8 A mixture of (2R)-4-(benzyloxycarbonylmethyl)-1-[3,5-bis(trlfluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-piperazine (1.86 g), 10~ Pd charcoal (0.186 g) and tetrahydrofuran (93 ml) was stirred for 17 hours under hydrogen gas atmosphere (1 atm). The catalyst was removed by filtration and the filtrate was concentrated. The residue was 'riturated with ethvl ethe- to give (2R)-4-(carboxymethyl)-1-[3,5-bis(trifluoromethyl)ber.zoyl]-2-(lH-indol-3-ylmethyl)piperazine (0.83 g) as a white powder.
[~]19 : -3.0~ (C=0.5, DMF) mp : 152-156~C
IR (Nujol) : 3600-3100, 1654, 1630, 1277, 1196, 1130 cm-1 NMR (DMSO-d6, o, : 2.20-5.2C (llH, m); 6.60-8.20 (8H, m); 10.85 (lH, s) lYASS : 514 (M+1) Prepar~tion 9 To an ice-cooled mixture of (2R)-2-benzyl-1-[3,5-bis(trifluoromethyl)benzoyl]piperazine (0.3 g) and triethylamine (0.39 ml) in dimethylformamide (8 ml) was added 3-(chloromethyl)pyridine hydrochloride (0.12 g). The reaction mixture was stirred at the same temperature for 30 minutes and then at room temperature for 2 hours Additional triethylamine (0.39 ml) and 3-(chloromethyl)pyridine hydrochloride (0.12 g) were added and the resulting mixture was stlrred ove~night. The rezction mixture was filtered and the filtrate was concentrated and subjected to a chromatography on a silica gel eluting with a mixture of toluene and ethyl acetate (5:1). The eluent was treated with 4N hydrogen chloride in ethyl acetate solution to give (2R)-2-benzyl~ 3,5-bis(trifluoromethvl)benzoyl]-4-(pyridin-3-yl-methyl)piperazine dihydrochloride.
mp : 164-168~C
[a]D5 : +9.1~ (C=1.0, MeOH) IR (Nujol) : 3700-3100, 2700-2000, 1630, 1270, 1120, 900 cm-l NMR (DMSO-d6, o) : 2.80-5.40 (llH, m); 6.85-6.90 (lH, m)i 7.10-7.40 (4H, m); 7.46 (lH, s); 7.75 (lH, s);
7.90-8.00 (lH, m); 8.19-8.23 (lH, m); 8.66-8.70 (lH, m)i 8.88-8.91 (lH, m)i 9.09 (lH, s) MASS : 508 (M+l) (free) Preparation 10 O

W 096/37489 PCTtJP96/01335 To a stirred mixture of (2R)-2-benzyl-1-[3,5-bis(trifluoromethyl)benzoyl]piperazine (0.3 g) and 2-(lH-indol-3-yl)acetic acid (0.13 g) in dichloromethane (8 ml) containing triethylamine (0.25 ml) was added 2-chloro-1-methylpyridinium iodide (0.22 g) at room temperature under nitrogen atmosphere. After being stirred for 5 hours, the reaction mixture was diluted with dichloromethane and washed with 0.lN hydrochloric acid, aqueous saturated sodium bicarbonate solution and brine, and dried over magnesium sulfate. After removal of the solvent, the residue was purified by column chromatography on silica gel using chloroform-methanol (50:1) as eluent to give (2R)-2-benzyl-1-[3,5-bis(trifluoromethyl)benzoyl]-4-[2-(lH-indol-3-yl)acetyl]piperazine (0.34 g) as a white powder.
mp : 201-210~C
[a]D7 : ~27.6~ (C=1.0, MeOH) IR (Nujol) : 3270, 1630, 1276, 1115, 900, 737 cm~l NMR (DMSO-d6, o) : 2.60-5.00 (llH, m); 6.70-7.70 (12H, m); 8.10-8.20 (lH, m); 10.85-11.10 (lH, m) MASS : 574 (M+l), 417 Pre~r~t;on 11 To a solution of (2R)-1-[3,5-bis(trifluoromethyl)-benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (0.1 g) in dichloromethane (10 ml) was added 4N hydrogen chloride in dioxane solution (0.05 ml) at 0~C. The resulting mixture was stirred at the same temperature for 50 minutes and then concentrated under reduced pressure. The obtained powder was collected by filtration and washed with ethyl ether to give (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)piperazine hydrochloride (0.1 g).
IR (Nujol) : 3340, 1648 cm~l NMR (DMSO-d6, o) : 2.9-3.9 (8H, m)i 3.9-5.2 (lH, m);
6.57-7.50 (5H, m); 7.50-8.30 (3H, m); 9.40-10.00 (2H, m); 10.96 (lH, s) ~SS : 456 (M+1) (free) Pre~aration 12 To a stirred mixture of (2R) -4-(2-aminoethyl)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(3,4-dlmethylbenzyl)piperazine dihydrochlo~ide (110 mg), trlethvlamine (0.2 ml) in dlchlorometh2ne (10 ml) was added methanesulfonyl chloride (0.1 ml) at 0~C. After stirrlng for l hour, the rezction mlxture was poured lnto ice-waler an~ extracted with ethyl acetate. The extract was washed successively with zqueous saturated sodium bica~bonate solution and brine, and dried.
After evaporation of the solvent in vacuo, the residue was purified by column chromatography on a silica gel eluting with a mixture of dichloro~ethane and methanol (40:1) and treated with 4N hydrogen chloride in ethyl acetate solution to give (2R)-1-[3,5-bis~trirluoromethyl)benzoyl]-2-(3,4-dimethylbenzyl)-4-[2-(mesylamino)ethyl]piperazine hydrochloride (50 mg).
mp : >220~C
[a]22 : +0.2~ (C=0.5, DMF) = lR (Nujol) : 3350, 2700-2400, 1645, 1500, 1450, 1380 cm~1 NMR (DMSO-d6, o) : 2.10 and 2.18 (6H, 2 s); 2.7-5.2 (17H, m); 6~6-7.7 (5H, m); 8.1-8.2 (lH, m); 11.05-11.4 (lH, m) MASS : 566 (M+1) (free) ~x~le 1 To a stirred solution of (2R)-4-(carboxymethyl)-1-~3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-piperazine (1 g) in dry dimethylformamide (10 ml) was added l-hydroxybenzotriazole (0.29 g) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.41 g) at room temperature. After stirrin5 for 15 minutes at room temperature, 1-amino-4-methylpiperzzine (320 mg) was added and further stirred for 5 hours at the same temperature. The reaction mixture was poured into a solution of sodium hydrogencarbonate (1.8 g) in water ~100 ml) and extracted three times with 20 ml portions of ethyl acetate.
The organic layers were combined and washed with brine (30 ml). The organic layer was dried over magnesium sulfate and filtered and the solvenl was removed by rotary evaporator.
The crude product was purified by chromatography (silica gel, dichloromethane:methanol, 5:1) to afford (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-4-[N-(4-methyl-1-piperazinyl)carbamoylmethyl]-piperazine (0.94 g) as a yellowish powder.
IR (Nujol) : 3180, 1680, 1630, 1276, 1170, 1130, 1005, 897 cm~l NMR (DMSO-d6, ~) : 2.16 (3H, s)i 2.0-5.0 ~19H, m);
6.6-8.2 (8H, m); 8.47, 8.77 (lH, 2 s);
10.85 (lH, s) ~x~m~le 2 (2R)-1-[3,5-Bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-4-[N-(4-methyl-1-piperazinyl)carbamoylmethyl]-piperazine (10.89 g) and fumarlc acid (2.07 g) were dissolved in ethanol (50 ml) at 70~C. After cooling, the resulting solution was concentrated under reduced pressure to give a powder (13.18 g). The powder (9.68 g) was dissolved in 2-butanone (194 ml) at reflux temperature and the solution was allowed to stir at room temperatlre to afford crystals, which was collected by filtration and dried to give (2R)-l-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-4-[N-(4-methyl-1-piperazinyl)carbamoylmethyl]piperazine fumarate (7.94 g).
mp : 169.5-171~C
IR (Nujol) : 32~0, 1700, 1653, 1630, 1275, 1217, 1168, 1122, 973, 894, 730 cm~l NMR (DMSO-d6, o) : 2.23, 2.26 (3H, 2 s); 2.10-4.93 (19H, m)i 6.60 (2H, s); 6.54-8.23 (8H, m); 8.50, 8.85 (lH, 2 s); 10.85 (lH, s) ~x~mple 3 Compound~(If), namely (2R)-l-[3~5-bis(trifluoromethyl) benzoyl]-2-(lH-indol-3-ylmethyl)-4-[N-(4-methyl-1-piperazinyl)carbamoylmethyl]piperazine fumarate was also oblalned according to tne following scheme.

H H

CH30H ~ ~ ClCH2COCl - - HCl H2N C02~ H2N C~2CH3 mp : 224-225 C (dec) 0 ~ ~ CH2NE2 Cl C

mp : 243 GC (dec) CA 0222204l l997-ll-24 O ~ \ J CH3C~2H ~ LiAlH4 H 11 .- HN ~ N

mp : 111~C ~

~C02H

F3C I (COC1)2 H
~ ~ oclCF3 H ~ F3 F3C ~ C
mp : 221~C ~

Pd charcoal CF3~ BrCH2cO2c2H5 HC 0 2 NH 2 b~ 3 C_3~ NaOH CF3 ~

F3C~ ~" COZc2H5 F C ~ ~ A, ~ o H

m : 152-156~C

l)ClCO2CH~CH3)2 CF3 ~

2) H2N CH3 ~ 3 ~ ~ ~CH3 Compound (I) HO2CeCH CF3 ~ HO2 Cll H
l ~ - O ~ NCE3 HCCO2H
2-butanone ~ C ~ N ~ N~" e~N ~ N
O H
Compoun~ (If) Fx~le 4 A mixture of (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (120 mg), 4-chloromethyl-2-(2-methoxyethylcarbonylamino)thiazole (70 mg) and powdered sodium hydrogen carbonate (27 mg) in dry dimethylformamide was stirred for 5 hours and 20 minutes at 60~C. The reaction mixture was powered into water and the resulting precipitate was collected by filtration. The crude product was purified by column chromatography on silica gel eluting with a mixture of dichloromethane and methanol (30:1). The eluate was evaporated under reduced pressure and treated with 17.6%
hydrogen chloride in ethanol (0.12 ml) to give (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)-4-[[2-(3-methoxypropanoylamino)thiazol-4-yl]methyl]piperazine hydrochloride (140 mg).
[a]D2 : _34 oo (C=0 5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1275, 1130, 900 cm~l NMR (DMSO-d6, o) : 2.60-5.20 (18H, m); 6.60-8.21 (9H, m); 10.90-11.00 (lH, m); 11.20-12.00 (lH, m); 12.19 (lH, s) MASS : 654 (M+1) (free) Fx~m~le 5 The following piperazine derivatives (Table 1) were prepared by the similar manner to that of the each Example No. or Preparation No. defined in the "Process" column. The physical properties of the object compounds are shown after the table.

- 31 ~-)~ ~

O ~ -A-R4 Table 1 ExampleObject CompoundsStarting Process No. -A-R4 Salt Compound -CH2 / N Pr.(Pre-5-l) ~S ~ NHCOCH3HCl parat on~ Ex. 4 = 5-2)~S ~ NHCOOC2H5 HCl Pr. 6 Ex. 4 5-3) ~ ~ NHC ~ HCl Pr. 6 Ex. 4 ~ ~ NHCOC(CH3)3 HCl Pr. 6 - Ex. 4 5 5) CH2 ~ ~ ~ HCl Pr. 6 Ex. 4 Table 1 (continued) Example Object Compounds Starting p NO. - A - R4 Salt Compound 5-6) T~ ~NHCOC3H7 HC1 Pr. 6 EX. 4 S

5-7 ) ~NHCOC2H5 HC1 Pr. 6 EX . 4 s 5-8) ~S ~ NHCHO HCl Pr. 6 Ex. 4 5-9) -CH2~NHCOCH3 2HC1 Pr. 6 EX. 4 5-10 ) T~ H~1 Pr . 6 EX . 4 5-11) ~ Pr. 6 Pr. 9 5-12) T~ 2HC1 EX.5-11) Pr.ll ~S~ NH2 5-13) ~ HCl EX. 5-11) Pr. 12 5-14) T~ - Pr. 6 Pr.10 ~S NHCOCH3 Table 1 (continued) Example Object Compounds Starting Process ~ No. - A - R4Salt Compound 5-15) ~ ~ HCl Pr. 6 Ex. 4 'S NHCOCH3 5-16) ~ \~ HCl Pr. 6 Ex. 4 \S/ ~N-COC2H5 Physical properties of the compounds of the Example 5 :

Fxample 5-1) mp : 185-189~C
[~]D4 : 30.2~ (C=0.5, MeOH) IR (Nujol) : 3660-3100, 2800-2000, 1635, 1545, 1276, 1183, 1130, 900 cm~l NMR (DMSO-d6, o) : 1.36-5.10 (14H, m); 6.59-8.22 ~lOH, m); 10.90-11.00 (lH, m); 12.15 (lH, s) MASS : 610 (M+l) (free), 456 Fx~m~le 5-2) [a]2D2 : -33 4O (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2800-2000, 1715, 1635, 1555, 1274, 1130, 900 cm-l NMR (DMSO-d6, o) : 1.25 (3H, t, J=7.1Hz); 2.73-5.10 (13H, m); 6.60-8.30 (9H, m); 10.90-11.00 (lH, m);
11.81 (lH, br s) MASS : 640 (M+l) (free), 456 ~x~m~le 5-3) [~]D2 : -42.0~ (C=0.5, MeOH) IR (Nujol) : 3600-3100, 2750-2000, 1635, 1540, 1277, 1175, 1130 cm~l NMR (DMSO-d6, ~) : 2.73-5.20 (llH, m); 6.59-8.21 (14H, m); 10.90-11.00 (lH, m); 12.69 (lH, s) MASS : 672 (M+l) (free), 456 Fx~m~le 5-4) [a] D2 : -24.2~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1540, 1276, lliO, 1129, 900 cm~l NMR (DMSO-d6, ~) : 1.23 (9H, s)i 2.73-5.10 (llH, m);
6.50-8.20 (9H, m)i 10.80-11.00 (lH, m); 11.84 (lH, s) MASS : 652 (M+l) (free), 456 ~x~le 5-5) [a3 D2 : -35.8~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1540, 1276, 1170, 1130, 900 cm~l NMR (DMSO-d6, o) : 0.80-1.00 (4H, m); 1.90-2.00 (lH, m); 2.73-5.15 (llH, m); 6.60-8.21 (9H, m); 10.90-11.00 (lH, m); 12.43 (lH, m) MASS : 636 (M+l) (free), 456 F.X~ rl e 5-6) [a]D2 : -30 4~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1542, 1275, 1170, 1131, 900 cm~l NMR (DMSO-d6, o) : 0.89 (3H, t, J=7.4Hz); 1.56-1.67 (2H, m); 2.40-2.50 (2H, m); 2.73-5.15 (llH, m);
6.56-8.21 (9H, m); 10.90-10.94 (lH, m); 12.12 (lH, s) MASS : 638 (M+l) (free) Fx~m~le 5-7) [a]D2 : _34 o~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1543, 1277, 1170, 1130, 900 cm~l NMR (DMSO-d6, o) : 1.08 (3H, t, J=7.4Hz); 2.43-2.50 (2H, m); 2.73-5.15 (llH, m); 6.55-8.21 (9H, m);
10.90-10.94 (lH, m); 12.11 (lH, s) MASS : 624 (M+l) (free) ~xAm~le 5-8) [a] 22 : -15.2~ (C=0.5, MeOH) IR (Nujol) : 3600-3100, 2750-2000, 1635, 1278, 1172, 1130, 900 cm~l NMR (DMSO-d6, o) : 2.73-5.20 (llH, m); 6.60-8.52 (llH, m); 10.94 (lH, s) MASS : 596 (M+l) (free), 456 FxAm~le 5-9) [a] D2 : -20.6~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1276, 1170 1129, 900 cm~l NMR (DMSO-d6, o) : 2.09 (3H, s); 2.73-5.20 (llH, m);
6.60-8.20 (llH, m); 10~46 (lH, s); 10.91 (lH, s) MASS : 604 (M+l) (free) FxAm~le 5-10) [a] D2 : -13.0~ (C=0.5, MeOH) IR (Nujol) : 3650-3050, 2750-2000, 1685, 1636, 1524 1275, 1130, 900 cm~l NMR (DMSO-d6, o) : 1.31 (3H, t, J=6.5Hz); 2.24 (3H, s); 2.73-5.20 (13H, m); 6.66-8.25 (8H, m); 10.94 (lH, s), 12.71 (lH, s) MASS : 682 (M+l) (free) Fxam~le 5-11) [a] D8 : -20.8~ (C=0.5, MeOH) IR (Neat) : 3700-3000, 1615, 1515, 1272, 1125, 900 cm~l NMR (DMSO-d6, o) : 2.00-5.00 (13H, m); 6.38-8.20 (9H, m); 10.80 (lH, s) MASS : 568 (M+l), 456 ~x~m~le 5-1~) [a] D2 : -10.4~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1635, 1277, 1130 cm~l NMR (DMSO-d6, o) : 3.00-5.20 (llH, m); 6.60-8.30 (llH, m); 10.95 (lH, s) MASS : 568 (M+l) (free), 456 Fx~m~le 5-13) [a]D2 : -31.8~ (C=0.5, MeOH) IR (Nujol) : 3270, 2750-2000, 1637, 1531, 1279, 1124, 964 cm~l NMR (DMSO-d6, o) : 2.73-5.15 (14H, m); 6.60-8.25 (lOH, m); 10.89 (lH, s) MASS : 646 (M+l) (free), 568, 456 Fx~mRle 5-14) [a] D3 : 11.8~ (C=0.5, MeOH) IR (N~jol) : 3650-3100, 1625, 1543, 1275, 1130 cm~l NMR (DMSO-d6, o) : 2.09-2.11 (3H, m); 2.52-5.00 (llH, m), 6.63-8.20 (9H, m); 10.85 (lH, s); 12.07 (lH, s) MASS : 638 (M+l), 456 ~x~m~le 5-15) [a] D8 : -51.6~ (C=0.5, MeOH) IR (Nujol) : 3650-3100, 2750-2000, 1634, 1540, 1274, 1170, 1127, 900 cm~l NMR (DMSO-d6, o) : 2.31 (3H, s); 2.73-5.35 (llH, m);
6.63-8.25 (8H, m); 10.94-11.00 (lH, m); 13.20 (lH, CA 0222204l l997-ll-24 MASS : 611 (M+l) (free) Fx~ ~ le 5-16) [a]22 _23 ao (C=0.5, MeOH) IR (Nujol) : 3650-3000, 2750-2000, 1620, 1274, 1175, 1128, 900 cm-l NMR (DMSO-~6, o) : 1.10 (3H, t, J=7.2H7), 2.60-5.10 (16H, m)i 6.50-8.21 (9H, m)i 10.51 (lH, s); 11.50-11.90 (lH, br s) ~SS : 638 (M+l) (free) ~5

Claims (8)

1. A compound of the formula :

or fumaric acid salt thereof.

2. The compound of claim 1, which is the compound of the formula :

3. A process for preparing a compound of the formula :

or fumaric acid salt thereof, which comprises (1) reacting a compound of the formula :

or its reactive derivative at the carboxy group or a salt thereof with a compound of the formula :

or its reactive derivative at the amino group or a salt thereof to give a compound of the formula :

or fumaric acid salt thereof, or (2) reacting a compound of the formula :

or a salt thereof other than fumaric acid salt thereof with fumaric acid to give a compound of the formula :

4. A pharmaceutical composition comprising a compound of claim 1 as an active ingredient, in association with a pharmaceutically acceptable, substantially non-toxic carrier or excipient.

5. A method for treating or preventing Tachykinin-mediated dlseases which comprises administering an effective amount of a compound of claim 1 to human being or animals.

6. A compound of claim 1 for use as a medicament.

7. Use of a compound of claim 1 for manufacture of a medicament for treating or preventing Tachykinin-mediated diseases.

8. A compound of the following general formula :

wherein R1 is trihalo(lower)alkyl, R2 is trihalo(lower)alkyl, R3 is indolyl(lower)alkyl, -A-is -CH2- or , and -R4 is , or in which R5 is hydrogen or lower alkoxycarbonyl, R6 is hydrogen or lower alkanoyl, R7 is hydrogen, lower alkyl, lower alkanoyl, lower alkoxycarbonyl, lower alkoxy(lower)alkanoyl, cyclo(lower)alkylcarbonyl, aroyl or lower alkylsulfonyl, or its pharmaceutically acceptable salt.