CA2216609C - Production d'anticorps par recombinaison specifique a un site induite par la recombinase cre - Google Patents

Production d'anticorps par recombinaison specifique a un site induite par la recombinase cre Download PDF

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Publication number
CA2216609C
CA2216609C CA002216609A CA2216609A CA2216609C CA 2216609 C CA2216609 C CA 2216609C CA 002216609 A CA002216609 A CA 002216609A CA 2216609 A CA2216609 A CA 2216609A CA 2216609 C CA2216609 C CA 2216609C
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CA
Canada
Prior art keywords
cell
antibody
sequence
site
lox
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002216609A
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English (en)
Other versions
CA2216609A1 (fr
Inventor
Aya Jakobovits
Krisztina M. Zsebo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Amgen Fremont Inc
Original Assignee
Japan Tobacco Inc
Abgenix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/412,826 external-priority patent/US6130364A/en
Application filed by Japan Tobacco Inc, Abgenix Inc filed Critical Japan Tobacco Inc
Publication of CA2216609A1 publication Critical patent/CA2216609A1/fr
Application granted granted Critical
Publication of CA2216609C publication Critical patent/CA2216609C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Méthode de production d'une cellule exprimant un anticorps à partir d'une séquence génomique d'une cellule comprenant un locus d'immunoglobuline modifiée. Le procédé consiste à exploiter la recombinaison spécifique à un site par Cre ainsi que la recombinaison homologue. Il consiste à d'abord introduire un site lox dans un locus d'immunoglobuline et à utiliser ledit site comme cible pour l'introduction de modifications géniques par recombinaison in vivo induite par Cre.
CA002216609A 1995-03-29 1996-03-27 Production d'anticorps par recombinaison specifique a un site induite par la recombinase cre Expired - Lifetime CA2216609C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/412,826 1995-03-29
US08/412,826 US6130364A (en) 1995-03-29 1995-03-29 Production of antibodies using Cre-mediated site-specific recombination
PCT/US1996/004445 WO1996030498A1 (fr) 1995-03-29 1996-03-27 Production d'anticorps par recombinaison specifique a un site induite par la recombinase cre

Publications (2)

Publication Number Publication Date
CA2216609A1 CA2216609A1 (fr) 1996-10-03
CA2216609C true CA2216609C (fr) 2006-02-21

Family

ID=35929937

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002216609A Expired - Lifetime CA2216609C (fr) 1995-03-29 1996-03-27 Production d'anticorps par recombinaison specifique a un site induite par la recombinase cre

Country Status (1)

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CA (1) CA2216609C (fr)

Also Published As

Publication number Publication date
CA2216609A1 (fr) 1996-10-03

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CA2216609C (fr) Production d'anticorps par recombinaison specifique a un site induite par la recombinase cre

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Effective date: 20160329