CA2196920A1 - Process based on the inhibition of phosphatases for detecting and quantifying diarrhoetic shellfish poison - Google Patents

Process based on the inhibition of phosphatases for detecting and quantifying diarrhoetic shellfish poison

Info

Publication number
CA2196920A1
CA2196920A1 CA002196920A CA2196920A CA2196920A1 CA 2196920 A1 CA2196920 A1 CA 2196920A1 CA 002196920 A CA002196920 A CA 002196920A CA 2196920 A CA2196920 A CA 2196920A CA 2196920 A1 CA2196920 A1 CA 2196920A1
Authority
CA
Canada
Prior art keywords
inhibition
phosphatases
diarrhoetic
quantifying
detecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002196920A
Other languages
French (fr)
Other versions
CA2196920C (en
Inventor
Luis Miguel Botana Lopez
Mercedes Rodriguez Vieytes
Juan Manuel Vieites Baptista De Sousa
Francisco Leira Sanmartin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidade de Santiago de Compostela
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2196920A1 publication Critical patent/CA2196920A1/en
Application granted granted Critical
Publication of CA2196920C publication Critical patent/CA2196920C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Process for detecting and quantifying diarrhoetic shellfish poison (DSP) based on the inhibition of phosphatases. The presence of DSP-producing red waters contaminates the molluscs which have to be constantly controlled before they are commercialised. The present technique is the bioassay which comprises the administration of a mollusc extract to rats; the result is positive if the rat dies before twelve hours. Said method is slow, costly, erratic and lacks specificity. The process of the invention detects and quantifies the toxines by measuring the inhibition of phosphatases partially purified. The phosphate inhibition is quantified by using as support a microtitration plate, and eliciting the enzymatic activity with a phosphatase substrate which, according to its chemical characteristics, is converted into a fluorescent, luminescent or colour product. Said technique is fast, economical and reliable. The sector which can beneficiate from this invention is the mollusc canning and producing industry.
CA002196920A 1995-06-07 1996-05-24 Process based on the inhibition of phosphatases for detecting and quantifying diarrhoetic shellfish poison Expired - Fee Related CA2196920C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ESP9501137 1995-06-07
ES9501137A ES2093566B1 (en) 1995-06-07 1995-06-07 PROCEDURE FOR THE DETECTION AND QUANTIFICATION OF DIARRHEAL DINOFLAGELATED TOXINS (DSP (DIARRHOETIC SHELLFISH POISON) BASED ON INHIBITION OF PHOSPHATASES.
PCT/ES1996/000117 WO1996040983A1 (en) 1995-06-07 1996-05-24 Process based on the inhibition of phosphatases for detecting and quantifying diarrhoetic shellfish poison

Publications (2)

Publication Number Publication Date
CA2196920A1 true CA2196920A1 (en) 1996-12-19
CA2196920C CA2196920C (en) 2009-02-24

Family

ID=8290660

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002196920A Expired - Fee Related CA2196920C (en) 1995-06-07 1996-05-24 Process based on the inhibition of phosphatases for detecting and quantifying diarrhoetic shellfish poison

Country Status (8)

Country Link
EP (1) EP0790317B1 (en)
JP (1) JPH10507371A (en)
CA (1) CA2196920C (en)
DE (1) DE69628134T2 (en)
DK (1) DK0790317T3 (en)
ES (2) ES2093566B1 (en)
PT (1) PT790317E (en)
WO (1) WO1996040983A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09262099A (en) * 1996-03-28 1997-10-07 Aisin Seiki Co Ltd Detection of nucleic acid with phosphate of fluorescein derivative

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5180665A (en) * 1990-11-21 1993-01-19 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Method for quantitatively assaying the presence of diarrhetic shellfish poisoning toxins in marine samples

Also Published As

Publication number Publication date
DE69628134D1 (en) 2003-06-18
PT790317E (en) 2003-10-31
ES2093566B1 (en) 1997-07-01
DE69628134T2 (en) 2004-04-01
CA2196920C (en) 2009-02-24
ES2093566A1 (en) 1996-12-16
EP0790317A1 (en) 1997-08-20
EP0790317B1 (en) 2003-05-14
DK0790317T3 (en) 2003-09-08
ES2200063T3 (en) 2004-03-01
JPH10507371A (en) 1998-07-21
WO1996040983A1 (en) 1996-12-19

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Legal Events

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