CA2175202A1 - A method for stabilizing protein c or activated protein c and the stabilized composition obtained by said method - Google Patents

A method for stabilizing protein c or activated protein c and the stabilized composition obtained by said method

Info

Publication number
CA2175202A1
CA2175202A1 CA002175202A CA2175202A CA2175202A1 CA 2175202 A1 CA2175202 A1 CA 2175202A1 CA 002175202 A CA002175202 A CA 002175202A CA 2175202 A CA2175202 A CA 2175202A CA 2175202 A1 CA2175202 A1 CA 2175202A1
Authority
CA
Canada
Prior art keywords
protein
composition obtained
stabilizing
activated protein
stabilized composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002175202A
Other languages
French (fr)
Other versions
CA2175202C (en
Inventor
Kazumasa Miyata
Yoshinori Akimoto
Yoichi Ogata
Tomohiro Nakagaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Teijin Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP5292500A external-priority patent/JP2886061B2/en
Application filed by Individual filed Critical Individual
Publication of CA2175202A1 publication Critical patent/CA2175202A1/en
Application granted granted Critical
Publication of CA2175202C publication Critical patent/CA2175202C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4866—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)

Abstract

The present invention is directed to a method for stabilizing protein C or activated protein C and a preparation obtained by said method. Said method and preparation being applicable during procedures such as isolation and purification, lyophilization, heating, etc. or when stored. The method comprises adding to a salt buffer containing protein C or activated protein C and sodium ion at least one amino acid, and further adding either one or a combination of albumin and a non-ionic surfactant.
CA002175202A 1993-10-29 1994-10-27 A method for stabilizing protein c or activated protein c and the stabilized composition obtained by said method Expired - Lifetime CA2175202C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP292500/1993 1993-10-29
JP5292500A JP2886061B2 (en) 1993-10-29 1993-10-29 Method and composition for stabilizing protein C or activated protein C
PCT/JP1994/001804 WO1995011698A1 (en) 1993-10-29 1994-10-27 Method of stabilizing protein c or activated protein c and stabilized composition

Publications (2)

Publication Number Publication Date
CA2175202A1 true CA2175202A1 (en) 1995-05-04
CA2175202C CA2175202C (en) 2006-09-19

Family

ID=37056965

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002175202A Expired - Lifetime CA2175202C (en) 1993-10-29 1994-10-27 A method for stabilizing protein c or activated protein c and the stabilized composition obtained by said method

Country Status (1)

Country Link
CA (1) CA2175202C (en)

Also Published As

Publication number Publication date
CA2175202C (en) 2006-09-19

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Legal Events

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Effective date: 20141027