CA2172840A1 - Measuring method using whole blood sample - Google Patents

Measuring method using whole blood sample

Info

Publication number
CA2172840A1
CA2172840A1 CA002172840A CA2172840A CA2172840A1 CA 2172840 A1 CA2172840 A1 CA 2172840A1 CA 002172840 A CA002172840 A CA 002172840A CA 2172840 A CA2172840 A CA 2172840A CA 2172840 A1 CA2172840 A1 CA 2172840A1
Authority
CA
Canada
Prior art keywords
partner
examined
substance
whole blood
blood sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002172840A
Other languages
French (fr)
Other versions
CA2172840C (en
Inventor
Nobuhiro Hoshino
Michiko Kawamoto
Mitoshi Shimamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LSI Medience Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/JP1995/001510 external-priority patent/WO1996004558A1/en
Publication of CA2172840A1 publication Critical patent/CA2172840A1/en
Application granted granted Critical
Publication of CA2172840C publication Critical patent/CA2172840C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/02—Hepadnaviridae, e.g. hepatitis B virus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for measuring a substance to be examined, characterized in that a sufficient amount of a whole blood sample is brought into contact with an insoluble carrier coated with a first partner specifically bindable to said substance to be examined for a short period of time; a complex of said substance to be examined and said first partner is brought into contact with a second partner which is labeled and capable of specifically binding to said first partner, said complex being carried on said insoluble carrier; a resulting complex of said insoluble carrier-said first partner-said substance to be examined-said labeled second partner, and a portion without said resulting complex are separated from eath other; and a signal originating from said label contained in one of said separated portions is detected is disclosed. According to the present invention, the substance to be examined in the whole blood sample can be quickly measured, using the whole blood as a sample without pretreatment.
CA002172840A 1994-07-29 1995-07-28 Measuring method using whole blood sample Expired - Lifetime CA2172840C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP19720794 1994-07-29
JP6/197207 1994-07-29
PCT/JP1995/001510 WO1996004558A1 (en) 1994-07-29 1995-07-28 Measurement system using whole blood

Publications (2)

Publication Number Publication Date
CA2172840A1 true CA2172840A1 (en) 1996-02-15
CA2172840C CA2172840C (en) 2007-10-23

Family

ID=38650021

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002172840A Expired - Lifetime CA2172840C (en) 1994-07-29 1995-07-28 Measuring method using whole blood sample

Country Status (1)

Country Link
CA (1) CA2172840C (en)

Also Published As

Publication number Publication date
CA2172840C (en) 2007-10-23

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Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20150728

MKEX Expiry

Effective date: 20150728