CA2146639A1 - Novel class of phosphocholine derivatives having antifungal activity - Google Patents

Abstract

Certain phosphocholine derivatives having substantial antifungal therapeutic activity are disclosed. The phosphocholine derivatives may be chemically synthesized, enzymatically prepared or extracted from the plant Irlbachia alata. The phosphocho-line derivatives are useful in treating fungal infections including those which are dermatophytic, systemic, ophthalmic and vagi-nal.

Description

~ W094/08563 2 1 4 ~ 6 3~ PCT/US93/09623 NOVEL CLASS OF PHOSPHOCHOLINE DERIVATIVES
~VING ANTIFUNGAL Ac~ v~

This is a continuation-in-part application of U.S. patent application Serial No. 07/958,416, filed October 8, 1993, the entire disclosure of which is incorporated by reference.

1. Field of the Invention This invention relates to new classes of phosphocholine derivatives as well as to various methods for preparing these compounds -- including synthetic, enzymatic and extractive using certain plants. The phosphocholine derivatives of the invention are non-toxic and exhibit substantial antifungal activity in slowing fungal growth and in killing fungi.

2. Backqround of the Invention The plant species Irlbachia alata has been used as an anti-infective agent in the Peruvian Amazon region. The leaves are squeezed and the liquid is applied to infected skin sores. The same liquid from the leaves is applied to skin problems and skin fungal infections. It is utilized to treat vaginal yeast infections.
Irl bachia alata is one species of 10-12 species of the plant family Gentianaceae. These species occur in tropical South America especially in the Amazon and Negro River basins. The plants in the genus Irlbachia are generally low herbs characteristically with 3-5 plinerved leaves. The most consistent diagnostic feature for the genus is the pollen morphology.
A reference to Irlbachia alata and related species was made in 1775 by the French scientist Fusee TE SHEE~

W094/08563 ` " ' PCT/US93/0962 Aublet (Aublet, F. 1775, Histoire des Plantes de la Guiane Francoise, Didot, Paris). The ethnobotanical notes from this reference were subsequently compiled and republished in English. Aublet noted the following about two species in the genus Irlbac~ia:
Irlbachia alata The entire plant is bitter.
It is used to clear obstructions; I (Aublet) have used it with good results. The species is called "Bois creux" (Hollow wood) by the Creoles.
Irlbachia pururascens All parts of this plant are bitter. It is used as an apertif and to reduce fever.

3. SummarY of the Invention We have discovered a class of phosphocholine derivatives (Class I) having extraordinary antifungal activity.
Structurally, these compounds are phosphocholine derivatives (1 or 2-deacyl-phosphatidyl cholines) in which the 1 or 2-OH-group of the glycerol moiety has been glycosylated with glucose, galactose, arabinose, mannose, rhamnose or another sugar. The basic chemical structure may be drawn as follows:

S~ ~E 8HEET

214663~
W094/0~3 _ 3 _ PCT!US93/09623 R' R ~ ~
\ ~ C ~ / O \ / / C~ / CH3 wherein one of R or R' is a sugar moiety and the other is an acyl or sugar moiety.
The molecular backbone common to all members of this class of compounds is drawn above. The acyl-group can be any long-chain fatty acid, while the sugar unit can be any of the sugars commonly found in plants, including but not limited to glucose, galactose, arabinose, mannose, rhamnose, or another naturally occurring sugar.
We have additionally found a structurally related class of phosphocholine derivatives of similar or greater antifungal activity than the above-discussed class of phosphocholine derivatives (i.e., Class I).
one novel class of phosphocholine derivatives (Class II) having antifungal activity has the basic structure shown below:
_ _ A F ~ ~ A
B--\ ' / \D/¦\X--Q--y/¦\D/ \N/--B
O- O' T

where Q is C2 to C30 alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, or branched alkynyl;
Z is oxygen or sulfur; X and Y are independent oxygen, sulfur, CH2, CF2, or N-RI;

8U~i l l l Ll rE SHE~

wog4/~321~6 63 ~ . PCT/US93/096 ~

A, B, and T are independently alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, or branched alkynyl radicals of C1 to C20 chain lengths; are independently or together cycloalkyl or bridged cycloalkyl radicals of ring size C3 to C20, or cylcoalkenyl, bridged cycloalkenyl or cyclo(polyene)radicals of ring size C4 to C20, cycloalkynyl, bridged cycloalkeynl or cyclo(polyalkynyl)radicals of ring size C8 to C20;
D is oxygen, sulfur, CH2, CF2, or N-R2;
F is alkyl, alkenyl, alkynyl, branched alkyl, ~ranched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl or cycloalkynyl radicals contA;n;ng C1 to C20 carbon atoms;
'5 Rl and R2 are independently hydrogen, alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl, bridged cycloalkenyl or cycloalkynyl radicals con~; n; ng C1 to C20 carbon a~oms, or any protecting group described in the book "Protecting Groups in Organic Synthesis" by Theodora Greene and Peter G.M. Wuts.
Another class of phosphocholine derivatives (Class III) having antifungal activity has the following structures:

EB BB DD

M ¦ ,or ~^~'M,orM BB

where AA, BB, DD are independent of each other, ~ e~ual to each other, or interchanged as shown above, the central carbon atom can be either the R and S

S~ 11 LJ1E SHEET

~ WOg4J0~3 21 4 66 ~ 9~ . PCT/US93,09623 optical stereoisomer or a mixture of R and S
stereoisomers, and where AA, BB, and CC are defined as follows:
where AA, is A-J with A being attached to the carbon atom of the three carbon central unit and J is defined below;
BB is B-Y, with B being attached to the carbon atom of the three carbon central unit and Y is defined below:
DD is ~,,~D~W~E ~G~Q
Il Z
where W, E, G and Q are defined below;
A is oxygen, sulfur, CH2, CF2 or N-R~;
B is oxygen, sulfur, CH2, CF2 or N-R2;
D is oxygen, sulfur, CH2, CF2 or N-R3;
Y is alkyl, alkenyl, alkynyl, poly(alkenyl), poly(alkynyl), or poly(alkenoalkynyl) radicals comprised of C1 to C20 carbon atoms chain lengths, or alkanoyl, alkenoyl, alkynoyl, poly(alken)oyl, poly(alkyn)oyl or poly(alkenoalkyn)oyl radicals comprised of C2 to C20 chain lengths or alkyloxy, alkenyloxy, alkynyloxy, poly(alkenyl)oxy, poly(alkynyl)oxy, poly(alkenoalkynyl)oxy radicals comprised of Cl to C20 carbon atoms;

~JBSmUrE SHE~

2 1 4 6 fi 3 9 ~ t S PCI~/US93/Og62~

J is a furanose or pyranose radical of the type:

L~ ;/Y, , -- !

where X is oxygen, sulfur, CH2, CF2 or N-R4;
F, K, L and M are independently hydrogen, hydroxyl, protected hydroxyl (as described in the book "Protecting Groups in Organic Synthesis" by Theodora Greene and Peter G.M. Wuts), alkyloxy, thiol, 1 alkylthio, arylthio, alkylsulfonyl, arylsulfonyl, amino, ammonium, alkylamino, alkylammonium, dialkylamino, dialkylammonium, trialkylamino, trialkylammonium where the alkyl chain on nitrogen is comprised of Cl to C20 carbon atoms; or alkyl, alkenyl, or alkynyl radicals comprised of Cl to C20 carbon atoms.
Z is oxygen or sulfur E is oxygen, sulfur, CH2 CF2 or N~Rs;
G is alkyl, branched alkyl, cycloalkyl or bridged cycloalkyl radicals of Cl to C20 chain lengths;
Q is halogen, hydroxyl, protected hydroxyl utilizing any protecting groups described in the book "Protecting Groups in Organic Synthesis" by Theodora Greene and Peter G.M. Wuts, O-arylsulfonyl-, O-alkylsulfonyl- or O-(perfluoroalkyl)sulfonyloxy, amino, ammonium, alkylamino, alkylammonium, dialkylamino, dialkylammoniumj trialkylamino, trialkylammonium where the alkyl chains on nitrogen are Cl to C20, or Q=NR~R2R3, where R~, R2, or R3 can independently or together be a mixture of alkyl groups Sl.lBSllTU~E SHEET

~ W094/~63 2 1 4 6 6 3 9 PCT/USg3/09623 of C1 to C20 in chain length and a protecting group described in the book "Protecting Groups in Organic Synthesis" by Theodora Green and Peter G.M. Wuts, and Rl can equal R2, R2 can equal R3, or Rl can equal R3 which can equal R3;
Rl, R2, R3, R4 and Rs are independently alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl or cycloalkynyl radicals of C1 to C20 chain lengths, or any protecting group described in the book "Protecting Groups in Organic Synthesis" by Theodora Greene and Peter G.M. Wuts;
where Wl and W2 are P(-OR) (with R being phenyl, phenylmethyl, or negatively-charged oxygen), S=O, carbon, or sulfur, provided that if Wl is not P(-OR) W2 is P(-OR) and provided that if J is a furanose or pyranose radical then Wl is P(-OR).
A preferred subgroup of the above-described Class III of phosphocholine derivatives have the 20 following structures:
BB OR

\/\ Q
o BB
R10 1 ORl Q ~ O~l,O ~ o~l,o Il 11 O O

SL~ ITE SHEET

-~1~66~g W094/0~3 ,- t ' PCT/US93/096 ~

AzO ~ 0~0 O

o R20 \ \ \ \ \ \ \
o- OR1 R2~o~o ¦ o R~ O

R~
R~ ~ \ O ~ O~¦,O Q
R~ o where R~ is phenyl or phenylmethyl, hydrogen, or nil;
R2 is hydrogen, phenylmethyl, or any protecting group described in the book "Protecting Group in Organic Synthesis" by Theodora Green and Peter G.M.
Wuts which can be cleaved by hydrogenolysis;
AA, BB, and Q are as defined above where the central carbon atom of the three carbon unit is either the R optical isomer, the S optical - isomer, or any mixture of the two optical isomers thereof;

SUBSTlTUTE SHEET

W094/0~63 ' 21~4 ~ ~ ~ n ~ U~J~ PCT/US93/09623 g Another preferred subgroup of the above-described Class III of phosphocholine derivatives have the following structures:

R20 _~
~L~R2 R20~ \
~0 ~ ORl R30~ o ¦ ~/\

o R20 ~ ~/
o o R O - l R 1R 30 `~"~ OR, Il 11 O O

SUBSmUTE SHEET

W094/08~3 2 1 ~6 6 3 g ~ ~ PCT/US93/0962 ~

R20 o~!~o~Q
R2O ~ o ~ ORs .

R - -T ~ ~ OR1 where Rl is phenyl or phenylmethyl, hydrogen, or nil; R2 is hydrogen, phenyl methyl or any protecting group described in the book "Protecting Groups in organic Synthesis" by Theodora Greene and Peter G.M.
Wuts which can be cleaved by hydrogenolysis;
R3 is hydrogen or a protecting group as described in the book "Protecting Groups in Organic Synthesis"
by Theodora Greene and Peter G.M. Wuts.;
where the central carbon atom of the three carbon unit is either the R optical isomer, the S optical isomer, or any mixture of the two optical isomers thereof; and Q is defined above.
Still another preferred subgroup of the above-described Class III of phosphocholine derivatives have the following structures:

SUts;~ ITE SHEET

~ W094/08563 ~l ~ 6 6 3 ~ PCT/US93/09623 HO ~ OR1 J ~ Q

O ~d H OR2 where R~ is phenyl or phenylmethyl, hydrogen, or nil;
R2 is a protecting group as described in the book "Protecting Groups in Organic Synthesis" by Theodora Greene and Peter G.M. Wuts, or hydrogen if R1 is not hydrogen;
and Q is defined above.
We have further found a novel, generally applicable method for the synthesis of the above described broad classes of phosphocholine derivatives (Classes I, II and III).

4. Brief Descri~tion of the Drawinqs Fig. l is the FTIR spectrum of the composition comprising a phosphocholine derivative obtained from Irlbachia alata.
Fig. 2 is the proton NMR spectrum of the composition comprising a phosphocholine derivative obtained from Irlbac~ia alata in D20 at 400 mHz.
Fig. 3 is the FAB-/MB mass spectrum of the composition comprising a phosphocholine derivative obtained from Irlbachia alata.

5. ~etailed DescriPtion of the Invention The glysosylated lysolecithins of the invention can be prepared by synthetic methods or by enzymatic methods. The phosphocholine derivatives can be SUBSTITUTE SHEET

~14~3g W094/0~63 ~ PCT/US93/0962 prepared either by synthetic methods or by methods entailing extraction from plant materials.

5.1. Chemical SYnthesis of phos~hocholine derivatives A wide variety of compounds having accessible alcoholic functionalities can be glycosylated following the classic Koenigs-Xnorr methodology.
Bochkov, A.F. and Zaikov. G.E., Chemistry of the O-Glycosidic Bond. Pergamon Press, 1979. As part of the synthetic route to phosphocholine derivatives with sugar, all but the anomeric hydroxyl group of the sugar to be introduced are protected either as esters or ethers, while the anomeric hydroxyl is being replaced by a halogen. The aglycon-sugar linkage is then formed via alcoholysis. Finally, the protective groups are selectively removed.
In the present invention, benzyl ethers or the benzilidine moiety are are the preferred protecting group, since they can be selectively removed by catalytic hydrogenation, while leaving the sensitive acyl-glycerol linkage intact. The glycosidation requires silver, mercury (Helferich modification), or cadmium salts as catalytic halogen abstractor, in the presence of a dehydrating agent (Timell, T.E., Can.J.
Chem. 1964, 42, 1456; Dejter-Juszynsky, M. and Flowers, H.M., Carbohydr. Res. 1973, 30, 287;
Marousek, V., Lucas, T.J., Wheat, P.E., and Schuerch, C., Carbohydr. Res. 1978, 60, 85), and with or without auxiliaries such as crown-ethers. (Kn~chel,A. Ger, R., and Thiem, J. Tetrahedron Letters 197~, 551) More recent methodology makes use of the halogen-abstracting power of non-nucleophilic bases such as diisopropylethylamine and/or of molecular sieves in an ~~ anhydrous media. (Garegg, P.J. and Norberg, T., Carbohydr. Res. 1976, 52, 235) The following SUBSTITUTE SHEET

~ W094/08~3 2 ~ 4 6 6t3 ~ PCT/US93/09623 synthetic scheme is based on the latter reaction sequence:

OH OBn 5ROJ~ ~p~ ~¢~+

R = Palmitoyl-, Oleyl-, Palmitoleyl-, etc. 1) Et4NE~rtiPr2EtNlMol.Sieves; DCMITHF.
2) H IPd;EtOAclTHF.

OH OH
~,/

H~

RO~0~ ~ O N~

2-0-,B-D-Glucopyranosyl Iysolecithin The synthetic two-step scheme outlined above can be conducted with commercially available materials.
2,3,4,6-Tetrabenzyl- 2,3-dibenzyl-4,6-benzylidene-glucose can be converted into the l-bromo- or l-O-triflate compound by stAn~Ard methodology. Leroux, J.
and Perlin, A.S. Carbohydr. Res. 1976, 47, C8. The corresponding phosphocholine derivatives are available through AVANTI POLAR LIPIDS, Inc. All other reagents are available from ALDRICH. The methodology outlined above is also applicable to either 1-acyl or 2-acyl ~ (1-acyl detailed above).

SUBSTITUTE StlEET

W094/0~3 2 1 4 6 ~ 3 9 ~ ~ PCT/US93/096 ~

5.2. Enzymatic pre~aration of 1 or 2 qlYcosylated lysolecithins As an alternative to the synthetic sequence outlined above, an in vitro enzymatic glycosidation simulating the biosynthetic process will produce the desired compounds in comparable yields. The natural glycosidation catalysts are glycosyltransferases.
These enzymes operate with uridinediphospho-glycosides (UDP-sugars) as substrates and ATP as the energy source. While the enzymes have to be prepared from fresh plant material, UDP-sugars, ATP, as well as the respective phosphocholine derivatives are commercially available. This synthesis has the advantage of being essentially a one-step process with the high selectivity and yields expected from an enzymatic reaction. The following scheme describes the preparation of a glucoside. Other transferases, not specific to glucose, could be applied in the preparation of glycosylated lysolecithins with other sugars as well:

SUBSmUTE SHE~T

wog4 2~66~9.

H~ O
HO~ 1 HO
S OH p g O ~

R = Palmitoyl-, Oleyl-, Palmitoleyl-, etc.) Uridine-5 -diphospho-glucose (=UDP-glucose) 1)1-Glucosyl TransferasetATP;Phosphate Buffer 2) Sephadex LH-20 OH OH
OH /~ /
J~y ~< O
HoO~

~2 O~
RO~ 0 ~ ¦~ 0 ~ t~

2-0-~-D-Glucopyranosyl Iysolecithin 5.3. Total SYnthesis of Phosphocholine Derivatives A general synthetic method of synthesizing phosphocholine derivatives of the various structures described in section 3 is outlined as follows.
An alcohol is phosphorylated or glycosylated.
The product is subsequently deprotected. The deprotected product is then alkylated or esterified to produce the phosphocholine derivatives. The general SIJ~ JTE SHEET

W094/0~63 ~1 4 6 6 3 ~

scheme for this outlined synthetic method is shown below.

SUBSTITUTE SHEET

WO 94/08~;63 2 ~ 4 6 6 3 9 PCI/US93/09623 General Scheme for the Synthesis of Known and Novel Lysolecithins R2Y~, HO~o R1X,~O

O

HO HO
R2V~OH R1 X~OH

- ~ ~

R2Y ~ ~XR1 R2Y~ ~ ~ZR3 R1X~ ~ ,ZR3R1x~ ~ ~yR2 Glycerol Derivative 0 can be either the R or S optical isomer, racemic, or a mixture of R and S isomers ~ Implies that a number of synthetic transformations are required R ~ = Sugar, carbocyclic sugar, functionalized sugar derivative, etc.
R2 = Phosphate or phosphate isostere moiety R3 = alkyl, alkanoyl, alkenyl, alkenoyl, etc.
X, Y, and Z can be C, O, N, S independently or equal to each other SUBSTITUTE SHEET

2146~39 W O 94/08563 " ' PC~r/US93/0962 5.4. Methods of Use The phosphocholine derivative in Classes I, II
and III are all useful in treating fungal infection by the administration to a warm~blooded animal of a therapeutically effective a~ount of a phosphocholine derivative. The pharmaceutical composition comprising the phosphocholine derivative used for such administration may also contain pharmaceutically acceptable excipients and carriers.
Phosphocholine derivatives in Classes I and II are believed to be novel compositions.
In order to treat a fungal infection, the antifungal agent of Classes I, II and III may be administered to a warm-blooded animal intravenously, intraperitoneally, subcutaneously, intramuscularly, orally, topically, by aerosol, or combinations thereof.
The antifungal agent of phosphocholine derivatives in Class II can be a~ri n; stered intravenously in a range of about 0.1 to about 10 mg/kg The fungal agent of Class II can be administered intraperitoneally in a range of about 0.1 to about 10 mg/kg.
The fungal agent of Class II can be administered subcutaneously in a range of about 1 to about 20.
The fungal agent of Class II can be administered intramuscularly in a range of about 1 to about 20.
The fungal agent of Class II can be administered orally in a range of about 5.0 to about 30 mg/kg.
The fungal agent of Class II can be administered topically in a range of about.5.0 to about 15% by weight.

SUt~ 111 lJTE SHEET

~ wo g4/0~3 2 ~ 4 ~ B 3 9 PCT/US93/09623 The fungal agent of Class II can be administered by aerosol in a range of about 5.0 to about 30 mg/kg/day.
The above dosage ranges may need to be doubled for those phosphocholine derivatives in Class I and III with lower antifungal activity which are identical or similar to those in table 2 (see below).

6. ~traction of phosphocholine derivatives from plants Plants are not known to contain phosphocholine derivatives.
The general manner of chemical extraction from the plants can be summarized as follows.
The plant source material, such as the whole plant, the roots, leaves, stem and/or latex of the plant, is extracted with water and/or a water miscible solvent. The preferred solvents are alcohol of 1-3 carbon atoms or acetone. The aqueous extract is extracted with butanol. The butanol-soluble fraction is subjected to gel filtration (e.g., over Sephadex), reversed-phase column chromatography (e.g., C-8), or gel-permeation chromatography (e.g., divinyl benzene cross-linked gels) such as PL-GEL or membranes (e.g., an Amicon membrane) using water or water and a water miscible solvent, with or without a buffer, as the mobile phase. The water miscible solvent is preferably a 1-3 carbon alcohol, acetone or acetonitrile.
The useful phosphocholine derivatives containing compound is the fraction detected by NMR spectroscopy.

A specific member of the class of phosphocholine - derivatives of the present invention is SUBSTITUTE SHEET

W094/08563 2 1 4 68 3 ~ PCT/US93/0962~

2-palmitoyl-1-O-glycopyranos~llysolecithin shown below:

CH~H
Ho ~O 0~o HO ~ O ~ ~~O
OH ~ I

Z-PaImitoyl-1-0-glucopyranosyllysolecithin We have found that 2-palmitoyl-1-0-glucopyranosyllysolecithin is a relatively active antifungal agent similar in activity to L-a-Lysophosphatidyl inositol, discussed in Table 2 below.
We have found that one of the most active antifungal compounds has the following structure.

CH3~ CH2 0 1 0 0 1 0 CH2 CH3 CH3--N~ CH2 11 (CH2)22 11 CH2 N~CH3 1,22-docosan diol bisphosphocholine ester.

SUBSmUTE SHEET

~ W O 94/08563 2 1 4 6~ 3 9~ PC~r/US93/09623 6.1. Extraction We have isolated by chemical extraction 1,22-docosan diol bisphosphocholine ester, the active antifungal compound contained in the plant Irlbachia S alata. The leaves of Irlbachia alata were milled and 200g of the milled leaves was extracted with lL of dichloromethane/isopropanol (1:1 v/v) at room temperature for 24 hours. The extracted material was separated from the marc (i.e., residual of the plant after solvent extraction) and ~icc~rded~ The marc was then extracted with 1.5L of isopropanol/water (1:1 v/v) at room temperature for 24 hours. The marc was separated from the extract and discarded. The isopropanol/water (1:1 v/v) soluble extract was partitioned between water and ethyl acetate. The ethyl acetate phase was separated and discarded. The water soluble phase, after extraction with n-butanol, was then discarded. The n-butanol phase was subjected to filtration over two Sephadex LH-20 gel columns using 90% aqueous ethanol (for first filtration) and 20% aqueous acetone (for second filtration) as the mobile phases. 1,22-docosandiol bisphosphocholine ester was collected from the early fractions of each gel filtration.
We believe that several related genera are the same and/or closely related to the genus Irlbachia, and would have similar medicinal properties. One species from a closely related genus, Lisianthus nigrens is used in Mexico. The leaves are applied as a poultice to treat fungal infections of the skin, feet, ankles and hands. A decoction of the root is also taken orally as a "bitter" and as a febrifuge.
Another species Lisianthus alatus is considered to be ~~ the same as Irlbachia alata. Another species and genus of interest is Chelonanthus alatus. There are SUBSTITUTE SHEET

W094/0856~ 1 4 6 6 3 ~ ~ PCT/US93/096 several uses described for Chelonanthus alatus, including oral decoctions to treat smallpox, fevers and for gastric disturbances.

S 6.2. S~ectral Characteristics The isolated phosphocholine derivative fraction containing 1,22-docosandiol bisphosphocholine ester has the characteristic IR, proton NMR and FAB- mass spectra shown in Figs. 1, 2 and 3, respectively.
The IR spectrum has peaks at approximately 1060, 1220, 1475, 1600-1700, 2850, 2950 and 3400 cm~l.
The IH NMR spectrum has major peaks at ~ 1.2, 1.4, 1.7, 3.1, 3.5, 3.7 and 4.3.
The FAB-/MB mass spectrum has major peaks (>40~) at m/z 657, 612, 587, 586, 555, 493, 491, 475, 403, 277, 233, 201, 194, 179, 168, 165 and 163.
The high resolution mass spectrum (FAB+) has a molecular ion at 673.4669 amu.

6.3. Total Synthesis of 2-~almitoYl-l-O-qlucopYranosyllYsolecithin Ex~erimental Section Gener~l. Tetrahydrofuran (THF) was distilled from potassium/benzophenone; benzene, triethylamine, and methylene chloride, N-methylmorpholine, and benzyl alcohol were distilled from calcium hydride; 2-bromoethylphosphorodichloridate was prepared according to the procedure reported by Baumann et al Lipids, 17, 453 (1982) and was freshly distilled prior to use;
trifluromethanesulfonic anhydride was freshly distilled under inert atmosphere; 0-~-D-(Glucopyranosyl)trichloroacetimidate was prepared by the method of Schmidt. (a) R. R. Schmidt, J. Michael, Angew. Chem. Int. Ed Engl. (1980), 19, 731; (b) R. R.
~~ Schnmidt, J. Michael, TetraAedron Lett. (1984), 25, 821. Anhydrous dimethylformamide (DMF! was obtained SU~ 11~ ~TE SHEET

W094/08563 2 1 4 ~ 6 3 ~ PCT/US93/09623 from Aldrich. S-(+)-1,2-0-isopropylidene glycerol and R-(-)-1,2-O-isopropylidene glycerol were obtained from Lancaster. 2,3,4,6-Tetra-O-benzyl-D-glucopyranose was obtained from Sigma. Preparative thin layer chromatography plates was performed on Whatman 2000 TLC silica gel plates. Flash column chromatography was performed on Whatman 230-400 mesh silica gel using nitrogen pressure. IH andl3C NMR were provided by using a Varian 400 MHz spectrometer with chloroform as an internal reference unless otherwise noted. NMR shifts were expressed in ppm downfield from internal tetramethylsilane. Carbon 13 multiplicities as determined by DEPT experiments are reported in parentheses following the chemical shift value according to the following format: (o) for quaternary carbon, (1) for methine carbon, (2) for methylene carbon, and (3) for methyl carbons. NMR assignments were determined on the basis of COSY, HMQC, and HMBC
and DEPT experiments performed on selected intermediates. NMR coupling constants are reported in Hertz. Melting points were determined using a Buchi model 535 melting point apparatus and are uncorrected.
The synthetic routes for the total synthesis of 2-palmitoyl-1-0-glucopyranosyllysolecithin are outlined in the following diagrams and detailed in the subsequent discussion that refer to these diagrams.

SU~i I ~ ~ ~ITE SHEEr 214663~
WO 94/08~;63 ~ ~ PCI~/US93/0962 Scheme 1. Synthesis of the (S),SP-19~01: Preparation of the Regicisomeric Glycerol Alco~o~s BnO THF BnO
BnO~O + ~O~............... "" ~ BnO~Lo ~`~""' BnO~,OH TfO~ o -1 oC BnOB~~
R / 1 68%
60% HOAc ~ +
reflux, 90 mi~ anomer 85%/ 19%
BnO BnO
BnO~LO HO TBDMscl BnO~ LO HO
BnO~O ~OH ~ BnO~,O ~OTBDMS
BnO Imidazole, DMF BnO
2 88% / 3 /palmitic anhydride Et3N, DMAP
O ~ 97%
O ~ \ ~
2 o BnO~o BnO~,O`,~OTBDMS
BnO \ TBDMSCI
4\ TBAF 93% \ Imidazole, DMF
~,~OAc, THF

TBAF, THF BnO~
46% BnO~O
B~ /OH

BnO
3 0 BnO~O HO
8nO~O~O ~

BnO Imidazole, DM F
BnO~LO 8 ~ 43h, 95%
BnOB~,O~O

SU~i 111 ~ITE SHE~ET

~ WO 94/08~63 2 1 ~ ~ 6 3 ~ PCI'JUS93/09623 Scheme 2. Synthesis of the (R) SP-19501: Preparation of the Regioisomeric Glycerol Alcohols, BnO TH F BnO
BnO~Lo + O~................ "", ~ BnO~O O~
BnO~oH TfO~,o -1 0C BnOB~o~O
S / 8 65-70%
60% HOAc ~ +
reflux, 90 mi~ o~- anomer BnO~ BnO
BnO~O HO TBDMSCl BnO~LO
BnO~,O J~ OH ~ BnO~,O ~ OTBDMS
BnO \' `/ Imidazole, DMF BnO \/ V
9 90-95% 10 /mitic anhydride Et3N, DMAP
O ~/ 90-95%
BnO
BnO~Lo 2 0 Bno~o~oTBDMs ~ TBDMSCI
11 \ TBAF 87-96% \ Imidazole, DMF
~ OAc, THF

46% BnO~O O ~
BnO~,O~OH

BnO 1 2 BnO~LO HO
3o BnO~o~O~

BnO Imidazole, DMF
- BnO~ TBDMSO " rt, 94%
3 5 B~ V~

Sl.lts~ 111 ~JTE SHEET

wo 94,o85632 1 4 6 6 3 ~ PCr/US93/0962~

Scheme 3. Synthesio, cf (S) SP-19501 BnO ll 5BnO~LO
BnO~O ~OH o Cl ~ Br~ ,P~_cl ~ Et3N

2) reagent 5 ~3) BnOH, 43%

BnO~
BnO~O\~/ ~p, O Br 15 ~CH3)3N
/ BOMB, 55C, 24h, 45%

BnO
BnO~o BnOB~~`p'O
16 ~\N+(CH3)3Br H2, 60 ps~ quantitative MeOH, 24 h '~ O
BnO ll BnO~O ~ ~~ ~ \~ r B~/ ~ `P'-O
'~ ~\
(S) SP-19501 N+(CH3)3 SUBSmUTE SHE,ET

8~63 2 1 4 6 ~ 3`~ Pcr/US93/09623 Scheme 4. Synthesis oi (R) SP-19501 BnO ll 5 BnO~LO
BnO~O OH o C
12 \~) Br~ ~P~_cl ~ Et3N

\ 2) reagent 5 \~3) BnOH, 43%

BnO
BnO~LO ~ \ ~~
BnO~,,O~O~e,OOBn 1 7 --\B
/ (CH3)3N
/ BOMB, 55C, 24h, 21%
O
BnO ll BnO~Lo BnO~,O~O~ ,O~

18 ~\N+(CH3)3Br H2, 60 ps~ quantitative MeOH, 24 h \
~ O
BnO ll BnO~O
BnO ~,O~O~ ,O~

(R) SP-l 9501 N+(CH3)3 Su~ JTE SHEET

21466~g WO 94/08~;63 ~- ~ ' PCr/US93/0962 ~ 28 ~

~/ o o~H BnOH O
098 mol% 500' h~ol% o - \
BrCH2CH20pcl2 N-methylmor~holine~ BrCH2CH20P/ ~
0--RT, 62~i~ 45%

1 M H3PO4, THF
(C6H5)3CCI, 105 mol% RT, 17h, 80%
O OH i-Pr2EtN, 105 mol% o OH
BrCH2CH2OI / ~/ RT, 40h, 52.1% 1 OH
O2Bln O2On THF Palmitic anhydride, 110 mol%
15 RT, 3h Et3N, 110 mol%
92.6% DMAP, 20 mol%
o o~(CH2)14CH3 20 BrCH2CH2OI / ~ HCOOH/THF, 1:1 OBn RT, 2h, 72.7%
22 or cUso4/c6H6 o reflux, 2h, 66.2% ~
~ =O (CH2)14CH3 OBn BrCH2CH2OI / ~OH

150 mol ~/ C~ ' 115 mZ1%
CH2CH2 RT, 4h, 22%

B~o dBCH2)14CH3 3s BnO~O O~ ,OCH2CH2Br 17 o SUBSTITUTE SHEET

~ W094/0~63 2 1 ~6 6 3 9 PCT/US93/09623 (R) 2,3-O-Isopropylidene-l-O-trifluromethylsulfonyl--glycerol. A nitrogen-purged 250-mL three-necked roundbottomed flask fitted with a thermometer, stopper, and septum was charged with S-(+)-1,2-O-isopropylidene glycerol (1.0 g, 7.6 mmol) dissolved inbenzene (75 ml). Triethylamine (1.25 mL, 9.0 mmol) was injected into the solution, and the reaction mixture was chilled until a cloudy solution appeared.
Trifluoromethaneæulfonic anhydride (1.25 mL, 7.6 mmol) was then added, and the reaction was stirred for 30 minutes with the temperature maintained at 5C. The solution was then filtered through a bed of silica.
The filtrate was concentrated under reduced pressure at 30C to give an orange/brown oil (1.84 g, 7.0 mmol) in 92% yield which was used directly for the next step.

~2R) tl-0-(2,3,4,,6-Tetra-O-benzyl-~-D-glu~Gyy~nosyl)-2~3-o-isopropyli~ene] glycerol 1 2,3,4,6-Tetra-O-benzyl-D-glucopyranose (100 g, 0.182 mol) was dissolved in THF (1.4 L) and chilled to -10C in a nitrogen-purged 3-L three-necked morton flask fitted with a thermometer, stopper, and mech~n;cal stirrer. Sodium hydride 60~ in oil (16.1 g, 0.403 mol) was added in 4 increments over 10 minutes, and the solution was stirred for 30 minutes.
(R) 2,3-O-Iso~ylidene-l-O-trifluoromethylsulfonylglycerol (60.0 g, 0.227 mol) dissolved in THF (500 mL) was then dropped via an addition funnel into the reaction mixture over a 30 minute period. The solution was stirred at -10C for 7 hours. Methanol (200 mL) was added dropwise to - quench excess sodium hydride, the resulting brown solution was rotary evaporated under reduced pressure SlJ~S 111 ~ITE SHEET

W094/08563 2 1 4 6 6.3 g ~- PCT/US93/096 ~

and then the residue redlssolved in chloroform (750 mL). The organic la~ye~ was washed with water (2 x 750 mL). The combined aqueous layers were washed with chloroform (3 x 500 mL). Organic layers were pooled and rotary evaporated under reduced pressure to give a white solid which contained both ~ and ~-epimers of the desired product. The solid was triturated with diethyl ether to give a white solid of purely ~--product and a mother liquor which contained ~ and ~-epimers. The mother liquor was concentrated and flashchromatographed (silica gel, 20% ethyl acetate/he~ne). Yield of the solid white ~-epimer product (81 g, 0.123 mol) was 68%, mp 91-91.7C (lit 83-84C);IH-NMR (CDCl3) S7.4-7.29 (m, 18H), 7.20 (m, 2H), 4.96 (d, 2H J=10.8), 4.84 (t, 2H, J=10.8), 4.75 (d, lH, J=10.8), 4.65 (d, lH, J=12.4), 4.6-4.54 (overlapping dd, 2H, J=12H, J=10.4), 4.46 (d, lH, J=7.2, Hl'), 4.38 (p, lH, H2), 4.12-4.02 (m, 2H, H~"
H2), 3.89 (pseudo t, lH, J=7.2, Hlb), 3.79-3.6 (m, 5H), 3.50 (pseudo t, 2H), 1.46 (s, 3H), 1.40 (s, 3H); 13C-NMR (CDCl3) S138.529 (0), 138.370 (0), 138.066 (0), 138.013 (O), 128.432, 128.409, 128.129 ,128.015, 127.901, 127.810, 127.734, 127.666, 109.399 (0), 103.824 (Cl'), 84-631 (C3'), 82.120 (C2'), 77.713 (C4'), 75.748 (2), 75.058 (2),.74.891,74.853, 74.315 (2), 73.495 (Cl), 70.317 (2), 68.762 (C6'), 66.896 (C3), 26.880 (3), 25.386(3). Yield of the colorless, oily ~-epimer (23 g, 0.035 mol) was 19~; IH NMR (CDCI3) S7.4-7.24 (m, 18H), 7.14 (m, 2H), 4.98 (d, lH, J=10.8), 4.88-4.78 (m, 3H), 4.67 (d, lH, J=12),.4.62 (d, lH, J=11.6), 4.47 (d, 2H J=11.6), 4.37 (t, lH, J=6.4), 4.07 (pseudo pentet, lH), 3.96 (t, lH, J=8.8), 3.8-3.54 (m, 9H), 1.43 (s, 3H), 1.37 (s, 3H); 13C NMR
(CDCl3) S138.764 (0), 138.203 (0), 138.165 (0), 137.816 (0), 128.440, 128.387, 128.364, 128.030, 127.947, SU~ 111 ~TE SHEET

W094/0~3 2 1 1~ 6 ~ 9 PCT~US93/09623 127.916, 127.886, 127.696, 127.590, 109.422 (0), 97.482 (Cl'), 81.885 (1), 79.890 (1), 77.508 (1), 75.703 (2), 75.088 (2), 74.535 (1), 73.457 (2), 73.108 (2), 70.279 (1), 69.020 (2), 68.314 (2), 67.040 (2), 26.827 (3), 25.424 (3).

(2R) 1-0-~2,3,4,6-Tetra-O-benzyl-~-D-glucG~y~nosyl) gly¢erol 2. A 5-L three-necked morton flask fitted with a mech~nical stirrer, condenser, and stopper was charged with compound 1 (50 g, 76.2 mmol) in 60%
aqueous acetic acid (2.5 L). The acidic solution was refluxed for 1.5 hours at 103C and then cooled to room temperature. Distilled water (1.5 L) was added to the solution causing precipitation of a white solid. The acidic solution was extracted with methylene chloride (4 x 1 L) which was subsequently neutralized with sodium bicarbonate solution and concentrated to a white solid. Trituration with diethyl ether gave white product. The remaining mother li~uor was flash chromatographed (silica gel, 50% ethyl acetate/hexane~ to give white solid product.
The combined yield (61.9 g, 0.101 mol) was 83 %, mp 101.5-102.4C (lit 76-78C); IH NMR (CDCl3) ~.40-7.26 (m, 18H), 7.19 (t, J=3.5, 2H), 5.0-4.7 (m, 5H), 4.64-4.5 (m, 3H), 4.46 (d, lH, J=8.0, H~'), 4.0-3.60 (m, llH, H~'s, H2, H3, H3', H6b', H6~', H~', Hs'~H2~ 2-55 (s, 2H, OH's); 13C NMR (CDC13) 38.529 (0), 138.332 (0), 137.952 (0), 137.740 (0), 128.531, 128.550, 128.478, 128.189, 128.114, 128.091, 127.931, 127.871, 127.749,104.279 (Cl'), 84.654 (C3'), 82.158 (C2'), 77.819 (C41), 75.779 (2), 75.081 (2), 75.028 (2), 74.527 (Csl)~ 73.571 (2), 72.207 (Cl), 71.204 (C2), 68.883, (C6'), 63.353 (C3).

SU~ I ~JTE S~EET

W094/0856~ i 4 6 6 ~ ~ PCT/US93/096 ~28) tl-0-(2113,~,C-TQtr~-O-b~nzyl-~-D-gluc,G~ylO~nosyl)-3-o-t~rt-butyld~imethylsilyl] glycerol 3. In a nitrogen-purged 100-mL round-bottomed flask fitted with a septum was dissolved!diol 2 (9.0 g, 14.7 mmol), imidazole (2.05 g, 30.2~m~mol), and t-butyl dimethylsilylchloride (2.28 gq -15.1 mmol) in anhyd DMF
(45 mL). The reaction mixturë was stirred under nitrogen for 2.5 days, transferred to a 1-L separatory funnei, and methylene chloride (250 mL) and water (250 mL) were added. The aqueous layer was extracted with methylene chloride (2 x 250 mL) and then the combined organic layers were washed with water (2 x 100 mL).
After drying and concentration, purification by flash chromatography (silica gel, 33% ethyl acetate/hexane) gave a colorless oil (9.2 g, 12.6 mmol) in 88% yield;
H NMR (CDCl3) ~7.48-7.3 (m, 18H), 7.25-7.21 (m, 2H), S.00 (d, 2H, J=11.2), 4.89 and 4.88 (overlapping doublets, 2H, J=10.8, J=10.4), 4.83 (d, lH, J=11.2), 4.67 (d, lH, J-12.4), 4.60 and 4.59 (overlapping doublets, 2H, J=12.4, J=10.8); 4.50 (d, lH, J=7.6 H~'), 4.06-3.92 (m, 2H), 3.9-3.62 (m, 7H), 3.6-3.52 (m, 2H), 3.04 (s, lH, OH), 0.978 (s, 9H), 0.142 (s, 6H); l3C NMR
(CDCl3) ~188.552 (0), 138.385 (0), 138.005 (0), 137.960 (0), 128.455, 128.440, 128.121, 128.060, 127.931, 127.863, 127.772, 127.734, 127.696, 104.377 (Cl'), 84.692 (C3'), 82.219 (C2'), 77.804 (C4'), 75.771 (2), 75.073 (2), 74.959,(2), 74.717 (C5'), 73.541 (2), 73.078 (2), 71.060 (C2), 68.7~5 (C6'), 63.998 (C3), 25.970 (3), 18.668 (0), -5.299 (3).
(28) t1-0-(2,3,4,6-T6tra-O-benzyl-~-D glucopyranosyl)-2-O-palmitoyl-3-O-t-butyldimethylsilyl~ glycerol 4. A
nitrogen purged 500-mL round-bottomed flask fitted ~ with a septum was charged with compound 3 (9.3 g, 12.8 mmol) and palmitic anhydride (6.94 g, 14.0 mmol) in ~U~ JTE SHEET

dry THF (200 mL). Dimethylaminopyridine (316 mg, 2.6 mmol) and triethylamine (2.04 mL, 14.7 mmol) were added, and the reaction was stirred under nitrogen for 12 h. The mixture was then transferred to a 2-L
s separatory funnel, and diethyl ether (500 mL) and water (500 mL) were added. The aqueous layer was filtered through Whatman No. 1 paper and extracted with diethyl ether (2 x 500 mL). After drying over magnesium sulfate, the combined organic layers were concentrated and purifled by flash chromatography (silica gel, 14% ethyl acetate/hexane) to give a light yellow oil (12.1 g, 12.5 mmol) in 97% yield; IH NMR
(CDCl3) ~7.40 (br. s, 20H), 5.15 (5, lH), 4.98 (t, 2H), 4.84 (t, 2H), 4.76 (d, lH), 4.67 (d, lH), 4.59 (dd, 2H), 4.52 (d, lH), 4.13(dd, lH), 3.84 (m, 6H), 3.67 (dd, 2H), 3.49 (t, 2H), 2.32 (t, 2H), 1.61 (m, 2H), 1.25 (br. s, 24H), 0.98 (s, 9H), 0.97 (s, 3H), 0.14 (s, 6H). 13C NMR (CDCl3) ~73.280, 138.597, 138.438, 138.127, 138.096, 128.379, 128.356, 128.333, 128.083, 127.977, 127.863, 127.780, 127.605, 127.582, 103.831, 84.556, 81.984, 77.721, 75.695, 75.020, 74.876, 74.603, 73.488, 72.904, 68.754, 67.821, 61.661, 34.428, 33.950, 31.941, 29.717, 29.687, 29.649, 29.619, 29.497, 29.459, 29.384, 29.300, 29.148, 25.826, 24.953, 22.716, 18.268, 14.159, -5.375.

~2R) rl-o-(2~3~6-T~tr~-o-bensyl-~-D-glucG~nosyl!
2-opalmitoyl] glycerol 5.
Procedur~ A. Compound 4 (34.0 g, 35.1 mmol) was dissolved in THF (1.4 L) in a 3-L three-necked Norton flask fitted with a mechanical stirrer, thermometer, and a 500-mL addition funnel. The solution was chilled to OC, and a solution of tetrabutylammonium ~~ fluoride (TBAF) (520 mL, 1.0 M in THF) which was buffered to pH=6.5 with acetic acid was added dropwise SlJtw ~ JTE SHEET

W094/085~ 1 4 ~ ~ 3 g ~ PCT/US9~/096 ~

through the addition funnel. The reaction mixture was stirred for 11 h at OC, left to sit at -15C for 12 h, and stirred again for 4 h at rt. Water (100 mL) was added, and the solut'ion was concentrated to 200 mL
of solution. The concentrate was redissolved in methylene chloride (750 mL) in a 3-L separatory funnel and washed with water three times (750 mL, 2 x 500 mL). The combined aqueous layers were extracted with diethyl ether (500 mL). The combined organic layers were concentrated to give a red oil which was purified by flash chromatography (silica gel, 33-40% gradient of ethyl acetate/hexane). A white solid (28.0 g, 32.8 mmol) was obtained in 93% yield. IH NMR (CDCl3) ~7.36 (br. s, 20H), 5.06 (t, lH), 4.96 (dd, 2H), 4.84 (dd, 2H), 4.75 (d, lH) 4.59 (m, 2H), 4.53 (dd, lH), 4.45 (dd, lH), 4.14 (m, 2H), 3.91 (m, 2H), 3.78 (m, 6H), 2.80 (s, lH), 1.64 (m, 2H), 1.27 (br. s, 26H), 0.90 (t, 3H).
Proce~ure B. Compound ~ (500 mg, 0.52 mmol) was dissolved in THF (20 mL) in a 100-mL three-necked round-bottomed flask fitted with two stoppers and a septum. Glacial acetic acid (9.5 mL) was added, and the solution was chilled to 0C. A solution of TBAF
(5.16 mL, 1.0 M in THF) was syringed into the chilled solution, and stinting was continued at 0C for 8 h and then at rt for 25 hours. Methylene chloride (50 mL) was added, and the entire solution was transferred to a 250-ml separatory funnel where it was neutralized with lM disodium phosphate solution (2 x 75 mL). The combined organic layers were rotary evaporated under reduced pressure and the concentrate was purified by flash chromatography (silica gel, 25-40% gradient of ethyl acetate/hexane), yielding a colorless oil (424 - mg, 0.497 mmol, 95~) which later solidified upon st~n~;ng; IH NMR (CDC13) ~7.36 (br. s, 20H), 5.06 (t, SUBSmUTE SHEET

~ W094/08~63 2146~ Pcr/us93/o9623 lH), 4.96 (dd, 2H), 4.84 (dd, 2H), 4.75 (d, lH), 4.59 (m, 2H), 4.53 (dd, lH), 4.45 (dd, lH), 4.14 (m, 2H), 3.91 (m, 2H), 3.78 (m, 6H), 2.80 (s, lH), 1.64 (m, 2H), 1.27 (br. s, 26H), 0.90 (t, 3H).

~28) tl-0-~2,3~4~6-T~tr~-O-b~nzyl-~-D-glu~G~y~osyl)-3-O-palmitoyl] glycerol 6. Compound ~ (3.0 g, 3.1 mmol) was dissolved in THF (120mL) in a 250-mL three-necked round-bottomed flask fitted with a 60-mL
addition funnel, glass stopper, and septum. TBAF (54 mL, 1.0 M in THF) was added through the addition funnel over a 15 minute period. Glacial acetic acid (18 mL) measured in a graduated cylinder was then poured into the reaction mixture, and the solution was stirred for 45 minutes. The solution was concentrated under reduced pressure to approximately 30 mL of liquid and then redissolved in methylene chloride (150 mL). The organic layer was washed with water (3 x 120 mL) and neutralized with sodium bicarbonate solution (2 x 150 mL). The combined aqueous layers were extracted with methylene chloride (100 mL). The combined organic layers were dried over magnesium sulfate, filtered, and concentrated. The resulting dark red concentrate was purified by flash chromatography (silica gel, 25% ethyl acetate/h~A~e) to give 6 a colorless oil which corresponded to an upper TLC spot (1.3 g, 1.52 mmol) in 46% yield. IH NMR
(CDC13) ~7.36 (br. s, 20H), 4.95 (m, 2H), 4.86 (m, 3H), 4.64 (d, lH), 4,58 (m, 2H), 4.47 (d, lH), 4.16 (m, lH), 3.96 (dd, lH), 3.68 (m, 8H), 2.38 (t, 2H), 1.62 (m, 2H), 1.27 (br. s, 24H), 0.96 (t, 3H). Isolation of a lower TLC spot gave a white solid (400 mg, 0.469 mmol) in 15% yield which corresponded to compound 5; IH
NMR (CDC13) ~7.36 (br- s, 20H), 5.06, (t, lH), 4.96 (dd, 2H), 4.84 (dd, 2H), 4.75 (d, lH), 4.59 (m, 2H), SUBSmUTE SHEET

W094/08563 2 1 ~ 6 ~ 3 ~ ~ . PCT/US93/0962~

4.53 (dd, lH), 4.45 (dd, 1H), 4.14 (m, 2H), 3.91 (m, 2H), 3.78 (m, 6H), 2.80(s, lH), 1.64 (m, 2H), 1.27 (br. s, 26H), 0.90 (t, 3H).

Resilation of ~2R) [1-0-(2,3,~,6-Tetra-O-b~nzyl-~-D
glucG~y~anosyl)-2-o-palmitoyl] glycerol 5. In a nitrogen-purged 50-mL round-bottomed flask fitted with a septum was placed compound 5 (318 mg, 0.373 mmol) dissolved in DMF (8 mL). tert-Butyl-dimethylsilyl chloride (281 mg, 1.86 mmol) and imidazole (254 mg, 3.73 mmol) were added, and the solution was stirred for 22 h. Methylene chloride (50 mL) was added, and the reaction mixture was transferred to a 250-mL
separatory funnel. The organic layer was washed with water (50 ml), and then the aqueous layer was extracted with methylene chloride (2 x 50 mL). The pooled methylene chloride layers were washed with water (2 x 75 mL), dried over magnesium sulfate, and the filtered. The filtrate was concentrated and purifled by flash chromatography (silica gel, 14%
ethyl acetate/hexane) to give 4 as a yellow oil (239 mg, 0.247 mmol) in 66% yield.

Re~ilation of (2S) tl-O-(2,3,~,6-TetraDO-benzyl-~
-D-glU~G~ ~08yl)-3-0-palmitoyl~ glycerol 6. In a nitrogen-purged 25-mL round-bottomed flask fitted with a septum was placed compound 6 (176 mg, 0.206 mmol) dissolved in anhyd DMF (5 mL). tert-Butyldimethylsilyl chloride (155 mg, 1.03 mmol) and imidazole (140 mg, 2.06 mmol) were added, and the solution was stirred for 43 h. Methylene chloride (50 mL) was added, and the reaction mixture was transferred to a 250-mL separatory funnel. The - organic layer was washed with water (50 mL). The aqueous layer was extracted with methylene chloride (2 W O 94/08563 2 1 ~ 6 6i3 ~ PC~r/US93/09623 x S0 ml). The methylene chloride layers were pooled methylene and washed with water (2 x 75 mL), dried over magnesium sulfate, and then filtered. The filtrate was concentrated and flash chromatographed (silica gel, 14% ethyl acetate/hexane) to give 7 as a light yellow oil (190 mg, 0.223 mmol) in 95% yield; lH
NMR (CDC13) ~7.38 (br. s, 20H), 4.99 (dd, 2H), 4.85 (t, 2H), 4.78 (d, lH), 4.68 (d, lH), 4.61 (dd, 2H), 4.48 (d, lH), 4.37 (d, lH), 4.13 (s, 2H), 3.98 (m, lH), 3.77 (m, 2H), 3.67 (m, 3H), 3.52 (m, 2H) 2.35 (t, 2H), 1.67 (m, 2H), 1.31 (br. s, 24H), 0.93 (s, 12H), 0.14 (s, 6H).

(2S) tl-0-(2~3~6-Totra-O-bensyl-~-D-glu~G~y~no~yl)-2-O-palmitoyl 3-0-(2-bromoethyl)benzylphosphoryl]
glycerol 15.
Procodure A. In a nitrogen-purged 100-mL three-necked round-bottomed flask fitted with two stoppers and a septum was dissolved freshly distilled 2-bromoethylphosophorodichloridate (1.72 g, 7.11 mmol)in diethyl ether (20 mL). The solution was chilled to 0C, and triethylamine (8.15 mL, 58.5 mmol) was injected into the solution which caused precipitation of a white solid. A solution of compound 5 (1.0 g, 1.17 mmol) in anhyd diethyl ether (55 ml) was injected into the chilled reaction mixture, and the ice bath was removed. The reaction was stirred for 30 minutes after which benzyl alcohol (1.21 mL, 11.7 mmol) was injected into the reaction mixture. Stirring was continued at rt for 5 d. The reaction was then filtered through a fritted glass funnel, and the filtrate was concentrated. The orange concentrate was purified by flash chromatography (0-33% ethyl - acetate/hexane) to give 15 as a light yellow oil (566 mg, 0.501 mmol) in 43% yield; IH NMR (CDCl3) ~7.38-7.25 SVBSTITUTE SHEET

WOg4/08563 - PCT/US93/0962 . .
(br. s, 23H), 7.16~;tm, 2H), 5.26 (m, lH), 5.10 (t, 2H), 4.94 (m, 2H), 4.81 (t, 3H), 4.71 (d, lH), 4.61 (d, lH), 4.55 (d, 2H), 4.39 (d, lH), 4.25 (m, 4H), 4.08 (dd, lH), 3.73 (m, 3H), 3.64 (dd, 2H), 3.42 (m, 4H), 2.27 (t, 2H), 1.58 (m, 2H), 1.25 (br. d, 24H), 0.89 (t, 3H).; ~3C NMR (CDCl3) ~173.210, 138.559, 138.362, 138.096, 138.074, 128.667, 128.622, 128.333, 128.318, 128.296, 127.962, 127.878, 127.757, 127.734, 127.696, 127.605, 127.522, 103.862, 84.540, 81.969, 71.652, 75.589, 74.937, 74.906, 74.686, 73.480, 70.469, 70.385, 69.680, 69.619, 68.777, 67.283, 66.099, 66.069, 34.170, 31.887, 29.657, 29.619, 29.596, 29.452, 29.315, 29.239, 29.080, 24,802, 22.647, 14.050.
Proce~ure B. In a nitrogen-purged 100-mL three-necked roundbottomed flask fitted with a thermometer, stopper, and septum was dissolved freshly distilled 2-bromoethylphosphorodichloridate (1.42 g, 5.85 mmol) in methylene chloride (15 mL). The solution was chilled to 0C, and compound 5 (1.0 g, 1.17 mmol) and a solution of N-methylmorphiline (1.28 mL, 11.7 mmol) dissolved in methylene chloride (35 mL) was injected into the solution over a 10 minute period. The reaction mixture was stirred at 0C for 5.5 h at which point a new TLC spot which co-spotted with secondary alcohol 6 appeared. Stirring was continued for another 30 minutes, and benzyl alcohol (1.21 ml, 11.7 mmol) was injected into the reaction. After 6 days of stirring, the reaction mixture was transferred to a 500-mL separatory funnel, and methylene chloride (150 mL) and water (200 ml) were added. The layers were separated, and the organic layer was rotary evaporated under reduced pressure. The resulting oil was flash - chromatographed (silica gel, 33% ethyl acetate/hexane) to give 15 as a yellow oil (2 50 mg, 19%); lH NMR

SUBSmUTE SHEET

214663~
W094/0~63 PCT/US93/09623 (CDCl3) ~7.38 8-7.2 5 (br. s, 23H), 7.16 (m, 2H), 5.26 (m, lH), 5.10 (t, 2H), 4.94 (m, 2H), 4.81 (t, 3H), 4.71 (d, lH), 4.61 (d, lH), 4.55 (d, 2H), 4.89 (d, lH), 4.25 (m, 4H), 4.08 (dd, lH), 3.73 (m, 3H), 3.64 (dd, 2H), 3.42 (m, 4H), 2.27 (t, 2H), 1.58 (m, 2H), 1.25 (br. d, 24H), 0.89 (t, 3H).; l3C NMR (CDC13) ~
173.210, 138.491, 138.286, 137.990, 137.975, 128.720, 128.652, 128.387, 128.364, 128.015, 127.954, 127.878, 127.810, 127.780, 127.727, 127.681, 127.613, 103.854, 84.495, 81.923, 77.781, 77.546, 75.688, 75.020, 74.808, 74.747, 73.473, 70.438, 69.642, 68.633, 67.322, 66.759, 66.129, 34.178, 31.925, 29.702, 29.664, 29.641, 29.490, 29.422, 29.368, 29.285, 29.103, 24.802, 22.700, 14.198.
(28) ~1-0-(2~3~6-Tetra-O-benzyl-~-D-glu~opy~nosyl)-2-0-palmitoyl-3-O-phosph~tidylcholine] glycerol 16. A
45 mL Parr bomb equipped with a magnetic stirring bar was charged with a solution of phosphate 15 in toluene (10 mL). Condensed anhydrous trimethylamine (12 mL) was added quickly in one portion, and then the vessel was sealed and heated in an oil bath at 55C for 24 h.
The reaction mixture was concentrated to a viscous oil and triturated with ethyl ether, upon which a white precipitate formed. the precipitate was filtered off, washed with ether, and then the combined ethereal solutions were concentrated to a glassy solid.
Purification of this residue using preparative TLC
(2000 ~ double elution with 75%,12.5%,12.5% methylene chloride/reethanol/ hexanes gave inner salt 16 as a - glassy solid;

t28) ~-D-gluco~nos-l-yl-2-O-p~lmitoyl-3-O-phosphati~ylcholine] glycerol 8P-19501. A solution of phosphatidylcholine 16 (200.4 mg, 0.197 mmol) in SUBSTITUTE SHEET

wo 2146~.39.. ..
94~08~63 ~ PCT/US93/096 i . , reagent grade methanol (25 mL) was hydrogenated at 60 psi over 10% Pd/C (40 mg, 20 wt%). After 30 h, the catalyst was ffltered off through celite and the methanol wAching were combined and concentrated. The residue was dissolved in fresh methanol (25 mL) and resubjected to hydrogenation at 60 psi over 80 mg (40 wt%) of 10%Pd/C. After 48 h, the reaction was still incomplete. After filtration, washing of the catalyst, and concentration, the residue was subjected to hydrogenation using 400 mg (200 wt%) of Pd/C at 60 psi in methanol (25 mL). After 22h, the catalyst was filtered off through celite and the methanol filtrate and washings were combined and concentrated to afford 92.8 mg (71.6%) of (8) 8P-19501 as a white solid; IH
NMR (CD30D) ~5.12 (br t, 0.5 H), 4.88 (br m, 4.5 H), 4.25 (br m, 2H), 4.12-3.57(M, 12H), 3.4-3.1 (m cont~in;ng singlet at 3.18, 12H), 2.3 (m, 2H), 1.55(m, 2H), 1.24 (m, 22H), 0.86 (br t, 3H); l3C NMR (CD30D) 74.93, 104.80, 78.02, 77.93, 75.19, doublet at 71.53 and 71.49, doublet at 70.80 and 70.73, doublet at 67.79 and 67.74, multiplet at 67.50, 62.53, doublet at 60.56 and 60.52, triplet at 54.79, 34.88, 33.15, 30.85, 30.85, 30.66, 30.56, 30.46, 30.26, 26.10 and 26.03, 23.82, 14.56; 3Ip NMR (CD30D) ~1.65.
~2S) 2,3-O-I~opropyiidene-l-0-trifluromethyl~ulfonyl-glycerol was prepared according to the method described for the corresponding (R) isomer in 92%
yield and used immediately.
~28) tl-0-~2~3~6-Tetra-O-benzyl-~-D-glu~o~y~nosyl)-2~3-o-isopropylia~n~] glycerol 8. 2,3,4,6-Tetra-O-benzyl-D-glucopyranose (65 g, 0.12 mol) was dissolved in THF (800 mL) and chilled to -10C in a nitrogen-purged 3-L three-necked morton flask fitted with a SU I i I UTE SHEEr ~ W094/08563 21.~ 6 ~ 3 D PCT/USg3/~K~3 thermometer, stopper, and mechanical stirrer. Sodium hydride 60% in oil (33 g, 0.825 mol) was added in 4 increments over 10 minutes, and the solution was stirred for lh. (S) 2,3-0-Isopropylidene-l-O-trifluoromethylsulfonylglycerol (0.15 mol) dissolvedin THF (200 mL) was then dropped via an addition funnel into the reaction mixture over a 20 minute period at -10 to -15C. The solution was stirred at -10 to -15C for 6 hours. The reaction mixture was filtered through a short plug of silica gel and concentrated to an orange brown oil, 114 g.
Purification of the crude by flash chromatography using 50% ethyl ether/hexanes gave 39.8 g (67.6%) of ~epimer 8 as a white solid, along with 4 g (5.1 ~) of a mixture of ~ and ~ epimers; mp of ~ anomer 85.7-87.2C; IH NMR of ~ epimer (CDC13) ~ 7.4-7.2 (m, 18H), 7.19-7.14 (m, 2H), 4.98-4.92 toverlapping doublets at 4.97 (J=10.8) and 4.94 (J=10.8), 2H], 4.82 (t, 2H, J-10.8), 4.73 (d, lH, J=10.4), 4.63 (d, IH, J=12.4), 4.58-4.51 [overlapping doublets at 4.55 (J=12) and 4.53 (J=10.8), 2H] 4.45 (dy lH, J=7.2), 4.36 (p, lH, H2), 4.08 (pseudo triplet, lH), 3.94-3.89 [overlapping doublets at 3.92 (J=10) and 3.91 (J=9.6), lH], 3.82-3.57 (m, 6H), 3.47 (pseudo triplet, 2H), 1.44 (s, 3H), 1.38 (s, 3H); ~3C NMR (CDC13) ~ 138.569 (0), 138.384 (0), 138.006 (0), 138.021 (0), 128.341, 128.258, 127.962, 127.856, 127.765, 127.696, 127.620, 127.605, 109.467 (o), 103.869 (Cl'), 84.586 (C3'), 82.075 (C2'), 77.705 (C4'), 75.680 (2), 75.005 (2), 74.815 (2 carbons, C2, C5'), 74.512 (1), 73.457 (Cl), 71.151 (2), 68.785 (C6'), 67.017 (C3), 26.895 (3), 25.393 (3).

- ~2 ) 1-0-~2,3,4,6-Tetra-O-bensyl-~-D-glucopyr~nosyl)--glycerol 9. A suspension of 8 (20 g, 30.5 mmol) in SU~S 111 ~TE SHEET

wo g4/08~3 2 1 q ~ ~ ~ 9 ~ PCT/US93/096 ~

60% acetic acid (800)~;was heated to reflux for lh.
Workup was similar`to`that described for the (R) diol 2, providing 18 g (96% yield) of 9 as a white solid, which was of sufficient purity after trituration with ether for the subsequent step. Diol 9 could be recrystallized from ether/hexane, mp 89.6-90.90C; IH
NMR (CDCl3) ~7.38-7.27 (m, 18H), 7.16 (t, J=3.5, 2H), 4.98-4.74 (m, SH), 4.61-4.5 (m, 3H), 4.42 (d, lH, J=8.0, H~'), 3.89-3.80 (m, 3H, H~'s, H2), 3.72-3.63 (m, 4H, H3 H3', H~'), 3.62-3.44 (m, 4H, H~', H4', Hs~ H2'), 2.59 (s, 2H, OH's); ~3C NMR (CDCl3) ~138.370 (0), 138.119 (0),137.78 (0), 137.69 (0), 128.462, 128.447, 128.432, 128.060, 128.038, 12i.962, 127.894, 127.848, 127.810, 127.704, 104.195 (C~'), 84.616 (C3'), 82.037 (C2'), 77.736 (C4'), 75.733 (2), 75.043 (2, 2 carbons), 74.466 (Cs~)~ 73.480 (2), 72.312 (Cl), 70.772 (C2), 68.731, (C6'), 63.355 (C3).

(2R) tl-0-~2~3~6-T~tra-O-b~nzyl-~-D-glucc~y~nosyl)-3-O-tert-butyldimQthylsilyl] glycerol 10. In a nitrogen-purged 100-mL round-bottomed flask fitted with a septum was dissolved diol 9 (28.0 g, 45 mmol), imidazole (5.71 g, 90 mmol), and t-butyl dimethylsilylchloride (6.92 g, 45.3 mmol) in anhyd DMF
(75 mL). The reaction mixture was stirred under nitrogen ovemight, transferred to a 1-L separatory funnel, and chloroform (300 mL) and water (300 mL) were added. The aqueous layer was extracted with chloroform (2 x 100 mL) and then the combined organic layers were washed with water (3 x 100 mL). After = drying (Na2SO4) and concentration, purification by flash chromatography (silica gel, 50% ethyl ether/hexanes) gave 10 as a colorless oil (29.5 g) in 90% yield;

SVBSTITUTE SHEET

~ WO ~/U~3 2 1 ~6 3~ PCT/US93/096Z3 ~2R) [1-0-(2~3~6-TQtra-O-b~nsyl-~-D-glu~G~ ~nosyl)-2-opalmitoyl-3-o-t-butyldim~thylsilyl] glycerol 11. A
mixture of 10 (22.2 g, 3 0.4 mmol), palmitic anhydride (16.5 g, 33.4 mmol), dimethylaminopyridine (741 mg, 6.08 mmol), triethylamine (3.78 g, 5.2 mL, 37.3 mmol) and anhyd THF (250 mL) was stirred under nitrogen at rt overnight. The mixture was poured into a 2-L
separatory funnel, diluted with diethyl ether (500 mL) and water (500 mL), and the layers separated. The aqueous layer was filtered through Whatman No. 1 paper and extracted with more diethyl ether (2 x 500 mL).
The combined ether layer was washed with water (3 x 200 mL) and then dried (MgSO4). Following filtration, purification by flash chromatography (silica gel, 33 ethyl ether/hexane) gave 11 as a light yellow oil, 28.2 g, 96~ yield);
Compound 11 could be carried on to the next transformation without chromatographic purification.

t2S) [l-o-~2~3~6-Tetra-o-benzyl-~-D-glu~G~ylanosyl) 2-Opalmitoyl] glyc~rol 12. Crude 11 (30.4 mmol based on 10) was dissolved in THF (100 mL) and the solution was chilled to OoC. A premixed solution of TBAF (520 mL, 1.0 M in THF) which was buffered to pH=6.37 with acetic acid was added dropwise via an addition funnel at 0C for 1 h, and then at -15C overnight. The reaction mixture was concentrated, water (100 mL) was added, and the resulting mixture was extracted with chloroform (3 x 300 mL). The combined chloroform layer was washed with water (4 x 500 ML), and then the - combined aqueous layer was backextracted with diethyl ether (500 mL). After drying the combined organic layer over Na2S04, concentration gave a red oil which - was purified by flash chromatography (~0% ethyl ether/hexane). Evaporation of the product containing SUBSTITUTE SHEET

W094/08563 ` PCT/US93/096 fractions afforded 12 as a white solid (24.6 g, 94.6%
yield for two steps);

~2R~ [1-0-(2,3,~,6-T~tra-O~-be~zyl-~-D-gluoG~yl~nosyl) 3-Op~lmitoyl~ glycerol 13. Compound 11 (2.33 g, 2.41 mmol) was dissolved in THF (150 mL) in a 250-mL three-necked round-bottomed flask fitted with a 60-mL
addition funnel, glass stopper, and septum. After cooling the solution to OoC, TBAF (24.1 mL, 1.0 M in THF) was added through the addition funnel over a 5 minute period. Glacial acetiç acid (13.8 mL 241 mmol) was then poured into the reaction mixture to quench the reaction, and the resulting solution was stirred for approximately 30 minutes. The reaction mixture was poured into a separatory funnel cont~;ning ice water (500 mL) and methylene chloride (200 mL). The layers were separated, and aqueous layer was extracted twice more with methylene chloride (100 mL portions) and then the combined organic layer was washed with brine (400 mL). Following dring (MgSO4), flltration, and then concentration, purification by flash chromatography using 1/5 EtoAc/ hexanes gave secondary alcohol 13, 0.96 g (46.8%), as a colorless oil;
Further elution gave 238 mg (11.6%) of primary alcohol 12; Also isolated was a mixture of the two alcohols in 5.3% yield.

Resilation of (28) tl-0-(2,3,~,6-T~tra~O-benzyl-~
-D gluoG~anosyl)-2-O-palmitoyl] glyc~rol 12. The identity of 12 was established by resilylation of 12 according to the procedure described above for the (R) isomer, compound 5.

SUBSTITUTE SHEET

~ W094/08~63 2 1 ~ 6 6 3 9 PCT/US93/09623 ~2R) tl-O-(2,3,~,6 ~L ~-O-benzyl-~-D-gluoo~.~nosyl)-2-o-palmitoyl-3-o-(2-bromoethyl)benzylphosphoryl]
glycerol 17. In a nitrogen-purged 1-L three-necked morton flask fitted with two stoppers and a septum was dissolved freshly distilled 2-brorhoethylphosphorodichloridate (17.2 g, 71.1 mmol) in anhyd diethyl ether (500 mL~. The solution was chilled to 0C and triethylamine (81.5 mL, 0.585 mol) was injected into the solution, causing precipitation of a white solid. A solution of 12 (10.0 g, 11.7 mmol) dissolved in diethyl ether (250 mL) was cannulated into the morton flask, and the solution was stirred for 1.5 hours. TLC showed disappearance of 12. Benzyl alcohol (12.1 mL, 0.117 mol) was injected into the reaction mixture, and stirring was continued at rt for 16 h. The reaction mixture was then filtered through a fretted glass funnel. Filtrate was then concentrated and purified by flash chromatography twice. First chromatography (silica gel, 33% ethyl 20 acetate/hexane) and second chromatography (silica gel, 25% ethyl acetate/hexane) gave 17 as a light oil (5.5 g) in 42% yield;

~28) [1-0-~2,3,4,6-Tetra-O-benzyl-~-D-glucG~l~nosyl)-2-Op~lmitoyl-3-O-pho~phatidylcholinel glycerol 18. A
45 mL Parr bomb was equipped with a magnetic stir bar and then charged with a solution of 17 (1.17 g, 1.04 mmol) in benzene (15 mL).
Anhyd trimethylamine (;5 mL, 0.145 mmol) which had been condensed at -78C was quickly poured into the reaction vessel, and the bomb was sealed. The reaction was stirred at 55C in an oil bath for 24 hours behind a blast shield. The bomb vessel was then - cooled to -78OC, opened, and left in a hood to evaporate trimethylamine. The remaining solution was 3UBSmUTE SHEET

w094~08s6~1 4 ~ ~ 3 9 - ~ PCT/US93/096 ~

rotary evaporated under reduced ~ressure, and the oily concentrate was dissolved in;methylene chloride and purified by preparative TLC ~2000 ~). Double elution with 75%; 12.5%:12.5% methylene chloride/methanol/hexane gave inner salt 18 as an opaque glassy solid (223 mg, 21%). IH-NMR (CDC13) ~.32 (br. s, 20H), 5.21 (m, lH), 4.90 (dd, 2H), 4.82 (m, 2H), 4.64 (m, 2H), 4.50 (t, 2H), 4.42 (d, lH), 4.22 (br. s, 3H), 3.95 (s. 2H), 3.72 (s, 2H), 3.62 (t, 2H), 3.55 (s, IH), 3.40 (m, 4H), 3.10 (s, 9H), 2.19 (m, 2H), 1.47 (m, 2H), 1.20 (br. d, 24H), 0.87 (t, 3H).
3C-NMR (CDC13) ~73.393, 138.453, 138.377, 138.051, 137.998, 128.470, 128.417, 128.356, 128.318, 128.235, 128.053, 128.007, 127.947, 127.856, 127.780, 127.719, 127.636, 127.567, 103.899, 84.472, 81.984, 77.478, 77.394, 77.311, 77.190, 75.672, 74.944, 74.550, 73.336, 68.663, 68.489, 59.158, 59.135, 54.409, 54.349, 34.246, 31.902, 29.710, 29.664, 29.535, 29.353, 29.330, 29.148, 24.7871 22.678, 14.121.
~2R~ D-glu~G~l~nos-1-yl-2-O-p~lmitoyl-3-O-phosph~ti~ylcholine] glycerol 8P-19501. A solution of phosphatidylcholine 18 (130 mg, 0.127 mmol) in reagent grade methanol (25 mL) was hydrogenated at 60 psi over 10% Pd/C (52 mg, 40 wt%). After 23 h, TLC showed an incomplete reaction. The catalyst was filtered off through celite and the methanol washings were combined and concentrated. The residue was dissolved in fresh methanol (25 mL) and resubjected to hydrogenation at 60 psi over 240 mg (185 wt%) of 10% Pd/C. After 20 h, the reaction was complete by TLC. The catalyst was filtered off through celite and the methanol filtrate and washings were combined and concentrated to afford - 64.0 mg (76.6%? of (R) 8P-19501 as a white solid; IH
NMR (CDC13) ~ 5.19 (m, lH), 4.97 (s, OH + HDO), 4.34-SUBSTITUTE SHEET

W094/08~63 2 1 4 6 ~ 3 g PCT/US93/09623 4.26 (br m, 2H), 4.16-3.95 (m, 3H), 3.9-3.6 (m, 6H), 3.42-3.14 (multiplet containing singlet at 3.24, 13H), 2.37 (t, J=7.6, 2H), 1.62 (pseudo t, 2H), 1.31 (m, 24H), 0.92 (t, J=7.2, 3H); l3C NMR (CD30D) 8175.02, 104.88, 78.07, 78.04, 75.04, 72.97, 72.89, 71.S2, 68.56, multiplet at 67.50, doublet at 64.99 and 64.94, 62.65, doublet at 60.52 and 60.48, triplet at 54.74 (J=3.1), 35.14, 33.13, 30.85, 30.69, 30.54, 30.29, 26.01, 23.79, 14.50; 31P NMR (CD30D) ~ 1.35 ~2R)1-[Benzyl-~2'bromoethyl)-pho~phproyl]-2,3-isopropylidene glycerol ~19).
2-Bromoethylphosphodichloridate (20.0 g, 0.08 mol) was dissolved in CC14 (50 ml) in a nitrogen-purged 0.5 L
three-necked flask fitted with a magnetic stir bar, thermometer, and a 125-ml addition funnel. The solution was chilled to 0C, and to this stirred solution was added dropwise the solution of (S)-form solketal (10.7 g, 98 mol %) and N-methyl-morpholine (8.22 g,98 mol %) in CCL4 (25 ml). After 2 hours TLC
showed disappearance of solketal. To the reaction mixture was added dropwise the solution of benzyl alcohol (44.6 g, 500 mol %) and N-methylmorpholine (8.38 g, 100 mol %). The reaction mixture was stirred under nitrogen for 60 hours at room temperature. TLC
showed the complete reaction. The reaction mixture was filtered through Shott filter #C, and the solution was rotary evaporated to volume near 70 ml and purified by flash chromatography (silica gel, diethyl ether) to give colorless oil (15.1 g, 0.04 mol) in 45 % yield; ~HNMR (CDCL3) ~ppm: 7.40 (br. s 5 H), 5.2 (d, 2 H), 4.3 (br.s, 3 H), 4.0 (br.s, 3 H), 3.85 (br.s,l H), 3.2 (s, 2 H), 1.4 (d, 6H); 13C NMR (CDCL3):
- 128.743, 128.682, 1~8.645, 128.114, 128.076, 109.885, 77.364, 77.046, 76.727, 73.920, 73.837, 69.771, Sl.l~ , JJTE SHEEr W094/08~63 2 1 ~6 ~ PCT/US93/096 69.710, 67.760, 67.707, 67.654, 66.774, 66.721, 65.955, 29.353, 29.277, 26.-6~3, 25.204; 31p NMR
(CDCL3):-1.108. ~-~2R)1-[Beuzyl-(2'-bromoethyl)-phosphoroyl] 1-2,3-~ihydroxy glyc~rol (20). A nitrogen purged 1 L round-bottomed flask fitted with septum was charged with compound 19 (19.5 g, 0.048 mol) in dry THF (50 ml) and the solution of 1 M H3PO4 (800ml) was added. The reaction mixture was stirred under nitrogen by room temperature for 15 hours. TLC showed the completness of the reaction. Then the reaction mixture was transferred to a 2 L sepapatory funnel. The acidic layer was extracted with ethyl acetate (7 x 450 ml).
The combined organic extract was washed with water (2 x 850 ml). After drying over sodium sulfate it was rotary evaporated and dryed in high vacuo for 10 hours to give a colorless oil (14 g, 0.04 mol %) in 80 %
yield; IH NMR (CDCL3), ~ppm: 7.38 (br.s, 5H), 5.2 (d, io 2H), 4.25-3.8 (multiplet, 6 H), 3.7-3.25 (br.m 5 H) l3C
NMR (CDCL3): 77.789, 77.774, 77.349, 77.030, 76.712, 70.522, 70. 491, 70.461, 70.431, 70.097, 70.044, 68.898, 68,883, 68.822, 67.085, 67.032, 62.617, 62.496, 42.363, 42.280; 31p NMR
(CDCL3): -0.485 (85 % H3PO4).

t2R)l-tBenzyl-~2~-bromoethyl)-pho~phoroyl-2-hydroxy-3 O-triphenylmethyl glycerol ~21). To a stirred solution of diol 20 (8.0 g, 21.6 mmol) in DMF (16 nil) was added diisopropylethylamine (4 ml, 105 mol %) followed by addition of trityl chloride ( 6.4 g, 105 mol %). After 40 hours at room temperature under nitrogen the reaction was complete by TLC. The ~ reaction mixture was diluted twice with water and extracted with diethyl ether (4 x 100 ml). The SUBSTITUTE SHEET

~ wo g4/08~63 ~ 1 4 6 6 3 ~ PCT/US93/09623 combined extract was dryed over sodium sulfate, concentrated and purified by flash chromatography silica gel, ethyl acetate:hexane,1:1) to give 21 as a light oil 6.9 g (52.1 %); IH NMR (CDCL3) ~.2-7.5 (br.m, 20 h), 5.07 (t, 2H), 4.12-4.26 (m,4H), 3.44 (dd 2H), 2.05 (s, lH), 1.26 (t, lH). 13C NMR (CDCL3): 138.772, 124.017, 123.911, 123.820, 123.342, 123.266, 123-152, 122.462, 122.417, 72.593, 72.274, 71.955, 65.105, 65.090, 65.030, 65.014, 64.954, 64.893, 62.109, 62.056, 58.877, 24.680, 24.604. 31p NMR (CDCL3) -0.158 (S) -t2R) 1-tBenzyl-t2'-bromoethyl)-phosphoroyl-2-O-p~lmitoyl-3-0-triphenyl methyl glycerol ~22). To a stirred solution of compound 21 (6.9 g, 11.3 mmol) in dry THF (90 ml) was added triethylamine (1.79 ml, 1 lO,mol %), palmitic anhydride (6.13 g, 1 10 mol%) and dimethylaminopyridine (276 mg, 20 mol%). The reaction was stirred under nitrogen for 3 hours untill TLC
showed disappearance of the starting material 21. The reaction mixture was rotary evaporated to a small volume and purified by flash chromatography (silica gel, diethyl ether:hexane, 1:3 to elute UV-nonactive impurities, diethyl ether:hexane,1:1 to elute compound 22). Yield 8.8 g (92.6%0, colorless oil, IHNMR (CDCL3) 7.41-7.22 (m, 20 H), 5.20 (d, lH), 5.04 (t, 2H), 4.23 (m, 4H), 3.58 (s, lH), 3.41 (s, lH), 3.23 (s, 2H), 2.33 (t, 2H), 1.62 (m, 3H), 1.24 (s, 24H), 0.88 (t, 3H). 13C NMR 172.984, 143.407, 143.285, 128.675, 128.607, 128.576, 128.523, 127.985, 127.848, 127.180, - 127.135, 86.672, 77.319, 77.000, 76.681, 70.901, 70.818, 69.604, 69.581, 66.463, 66.440, 61.828, 34.284, 31.894, 29.672, 29.634, 29.611, 29.437, ~~ 29.346, 29.285, 29.247, 29.232, 29.141, 29.095, 24.832, 22.678, 14.121. 32p NMR (CDCL3)-1.327.

SUBS~ITUTE SHEET

W094~08 ~ 1~ 6 6 3 9~ ~ r PCT/US93/096 ~

~2R)1-rBe~syl(2'- bromoethyl)-phosphoro~-yl-2-O-palmtoyl-3-hy~roxy glycerol 23. Proce~ure A To a stirred solution of compound 22 (3.2 g, 3.76 mmol) in 45 ml THF was added 45 ml 96~ formic acid. After 2 hours qt room temperature t~e rèaction was coplete by TLC. The reaction mixture was diluted twice with water, neutralized with sodium bicarbonate (3 x 300 ml). The combined extract was washed with water, dryed over sodium sulfate, rotary evaporated to a small volume and purified by flash chromatography (silica gel, ethyl acetat: hexane, 1:3 to. elute less polar impurities, ethyl acetat:hexane,1:1 to elute compound 23. Yield 1.65 g (72.7 %), colorless oil.
Procedure B. A nitrogen purged 0.5 L round-bottomed flask fitted with condenser was charged with compound 22 (1 g, 1.17 mmol) in dry benzene (230 ml) in the presence of anhydrous CuS04 (17.6 g). The reaction mixture was stirred at room temperature for 15 hours and then reflux for 2 hours untill the reaction was complete by TLC. The CuS04 was filtered off through Shott filter #C and concentrated in vacuo and purified by flash chromatography (silica gel, ethyl acetate/
hexane, 1:1) to give a light yellow oil (0.47 g, 0.77 mmol) in 66 % yield. IHNMR (CDCL3): ~7.40 (br. s, 5H), 5.2 (d, 2H), 4.2 (mult, 8H), 2.32 (t, lH), 1.62 (pseudo t, 2H ), 1.31 (m, 24H), 0.88 (t, 3H). 13C NMR
(CDCL3): 130.898, 128.872, 128.789, 128.698, 128.538, 128.S16, 128.114, 127.886, 126.968, 77.326, 77.008, 76.689, 70.097, 70.074, 69.012, 68.951, 68.633, 68,604, 68.572, 68.542, 67.085, 67.047, 67.032, 66.994, 65.272, 64.195, 62.731, 62.716, 34.041, 31.902, 29.672, 29.588, 29.505, 29.444, 29.338, 29.239, 29.103, 24.749, 22.670, 14.113. 31P NMR: -3.069 (85% H3P04).

SUBST~TUTE SHEET

~ W094/08~63 2 1 4~ ~ 3 ~ PCT/US93/09623 ~2R)-1-0-~2,3.~.6-T~tr~-O-b~nzyl-~-D-gluco-pyr~nosyl)-2'-O-p~lmitoyl-3~-o-[benzyl!2~-bromoethyl)-phosphoril]-glycQrol ~17). To a stirred solution of o-(~ -D-glucopyranosyl) trichloroacetimidate (2~) (390 mg, 115 mol %) in dry methylene chloride (3 ml) was added dropwise a solution of compound 6 (300 mg, 0.49 mmol) and boron trifluoride etherate (70 mg, 100 mol %) in dry methylene chloride (3 ml). The reaction mixture was stirred under nitrogen for 2 hours at room temperature, then more compound 24 (100 mg, 35 mol %) was added to bring the reaction to the end. After 4h, the reaction mixture was evaporated to a small volume and separated by flash chromatography (silica gel, diethyl ether/hexane, 1:3) to give compound 17 as a colorless oil (120 mg, 22%), which was identical to the material described earlier.

6.4. Antifungal Activity The antifungal activity of the isolated phosphocholine fraction was determined in vitro by using three fungal cultures - Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus.
The method used to determine in vitro antifungal activity is discussed in McGinnis, M.R., Laboratory Handbook of Medical Mycology, Academic Press, New York, London, p661 (1980); and Droughet E., Dupont, B., Improvisi, L., Vivian, M.A. and Tortorano, A.M., "Disc agar diffusion and microplate automatized tec-hnics for in vitro evaluation of antifungal agents on yeast and sporulated pathogenic fungi" in In Vitro and In Vivo Evaluation of Antifungal Agents, Eds.
Iwata, K. and Vanden Bossche, H., Elsevier Science Publishers, New York, Oxford p303 (1986).

SUBSTITUTE SHEET

21~6~39 W094/08~63 ' ~ PCT/US93/0962 The minimum ihhibitory concentration (MIC) and the minimum fungicidal concentration (~FC) are summarized in the table 1 below.

Fungus Culture MIC (uq/ml) MFC fuq/ml) C. albicans 0.8 C. neoformans < 0.1 A. fumigatus < 0.1 ~0.4-0.8 These results clearly indicate the significant antifungal activity of the isolated fraction containing against a variety of fungal cultures.

6.5. Antifunqal Activities of the Phosphocholine 4erivatives Class A series of related analogs to 2-palmitoyl-1-0-glucopyranosyllysolecithin obtained commercially from Avanti Biolipids have also been found to have high antifungal activities. A summary of the antifungal screening test is shown in table 2. The analog compounds were tested for their activity against C. albicans, C. neoformans, A. fumigatus and T.
rubrum. Partial inhibition of the fungus of between 25 to 75% was measured along with the total inhibition (MIC) by these anolog compounds. A description of the partial inhibition measurement can be found in R. L.
Stiller, et al The Journal of Infectious Diseases, 147, No. 6 (1983). The structure of these analog compounds is as follows.

SUBSTITUTE SHEET

~ W094/0~63 2 ~ 4 6 6 ~ ~ PCT/US93/09623 H

R0 \ CH / \ CH / \ p / \ /

wherein R is the group identified in table 2.

SUBSmUTE SHEEI

214663g WO 94/08~63 ' 5 4 PCI/US93/096~

o o o o o ,C E~
., o ` o o o - o ~ In Z _ o o ,~
-1 ~ V
_ o o o o U --~ ~

o o o o o o U~

o o o o o ,,~ o o o ~ U~ U~
V ~ ~ ~ ~ ~ ~1 ~1 :s$
o `t o o o o U~ U~
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SUBSTITIJTE SHEET

21~G639 WO 94/08563 5 5 ~ PCI/US93/09623 o _I
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a .

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,1 .

SUBSTITUTE SHEET

21~-G3~
W094/08~63 ~ ~ PCT/US93/096 6.6. ToxicitY
The toxicity of the isolated phosphocholine derivative fraction is low, based~on tests with Hep 2 cells indicating an ID50 of greater than 1000 ug/ml.
The method used in determining cytotoxicity is discussed in Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J. Immun.
Methods, 65, 55-63, 1986.
The isolated fraction having the above-described in vitro antifungal activity and low toxicity is expected to similarly exhibit significant in vivo antifungal activity against fungal infections which are dermatophytic, systemic, ophthalmic and vaginal.
Other human and animal infections treatable with the compounds of the present invention include aspergilliosis, candidiasis, and cryptococcus infections.
It is expected that the same isolated fraction would be useful in treating fungal infestation in plants as well.
It is apparent that many modifications and variations of this invention may be made without departing from the spirit and scope thereof. The specific embodiments described are given by way of example only and the invention is limited only by the terms of the appended claims.
A number of references are cited in the present specification, the entire disclosure of each of which is incorporated by reference herein, in its entirety.

SUBSTITUTE SHEET

Claims (21)

What is claimed is:

1. A phosphocholine derivative having the structure of:

wherein one of R or R' is a sugar moiety, and the other is an acyl or sugar moiety.

2. The phosphocholine derivative according to claim 1 wherein the sugar moiety is selected from the group consisting of glucose, galactose, arabinose, mannose, rhamnose, and other naturally occurring sugars.

3. The phosphocholine derivative according to claim 1 having the structure of:

2-Palmitoyl-1-O-glucopyranosyllysolecithin

4. An antifungal composition capable of inhibiting fungal growth when administered to warm blooded animals or plants comprising an effective amount of a phosphocholine derivatives having the structure of:

wherein one of R or R' is a sugar moiety, and the other is an acyl or sugar moiety.

5. A composition comprising a phosphocholine derivative obtained from Irlbachia alata characterized by:

(a) IR spectrum having peaks at approximately 1060, 1220, 1475, 1600-1700, 2850, 2950, and 3400 cm-1;
(b) 1H NMR spectrum having major peaks at .delta.
1.2, 1.4, 1.7, 3.1, 3.5, 3.7 and 4.3; and (c) FAB/MB mass spectrum having major peaks (>40%) at m/z 657, 612, 587, 586, 555, 493, 491, 475, 403, 277, 233, 201, 194, 179, 168, 165 and 163.

6. The composition according to claim 5 wherein the phosphocholine derivative is characterized by:
(a) IR spectrum substantially illustrated in Fig. 1;
(b) 1H NMR spectrum substantially illustrated in Fig. 2;
(c) FAB/MB mass spectrum substantially illustrated in Fig. 3.; and (d) HRMS (FAB+) spectrum having a molecular ion at 673.4669 amu.

7. A composition comprising a phosphocholine derivative obtained from Irlbachia alata by a method which comprises:
(a) extracting the whole plant, the leaves, the stems, the roots or the latex of the plant Irlbachia alata with a lower alcohol of about 1-3 carbons, acetone, water or other water miscible solvent or combinations thereof to obtain an aqueous soluble fraction;
(b) subjecting the aqueous fraction to butanol extraction and the butanol-soluble fraction to gel filtration using water and/or water and a water miscible solvent with or without a buffer as the mobile phase; or to reversed phase column chromatography using water, and/or water and a water miscible solvent as the mobile-phase;
or to gel permeation chromatography using water and/or water and water miscible solvent and acetonitrile with or without a buffer as the mobile phase; or combination thereof and (c) collecting the fractions detected by NMR spectroscopy.

8. A pharmaceutical composition which is useful in treating a fungal infection when administered to a warm-blooded animal a therapeutically effective amount of an antifungal agent comprising a phosphocholine derivative having the structure of:

wherein one of R or R' is a sugar moiety, and the other is an acyl or sugar moiety.

9. A pharmaceutical composition which is useful in treating a fungal infestation when administered to a plant comprising an effective amount of an antifungal agent comprising a phosphocholine derivative having the structure of:

wherein one of R or R' is a sugar moiety, and the other is an acyl moiety or sugar moiety.

10. A pharmaceutical composition which is useful in treating a fungal infection when administered to a warm-blooded animal comprising a therapeutically effective amount of an antifungal agent comprising a compound having the structure of:

where Q is C2 to C30 alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, or branched alkynyl;
Z is oxygen or sulfur; X and Y are independent oxygen, sulfur, CH2, CF2, or N-R1;
A, B, and T are independently alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, or branched alkynyl radicals of C1 to C20 chain lengths; are independently or together cycloalkyl or bridged cycloalkyl radicals of ring size C3 to C20, or cylcoalkenyl, bridged cycloalkenyl or cyclo(polyene)radicals of ring size C4 to C20, cycloalkynyl, bridged cycloalkenyl or cyclo(polyalkynyl)radicals of ring size C8 to C20;
D is oxygen, sulfur, CH2, CF2, or N-R2;
F is alkyl, alkenyl, alkynyl, branched alkyl, branced alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl or cycloalkynyl radicals containing C1 to C20 carbon atoms;
R1 and R2 are independently hydrogen, alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl, bridged cycloalkenyl, or cycloalkynyl radicals containing C1 to C20 carbon atoms, or a protecting group.

11. A pharmaceutical composition which is useful in treating a fungal infection when administered to a warm-blooded animal comprising a therapeutically effective amount of an antifungal agent comprising a compound having the structure of:

, or , or , where AA, BB, DD are independent of each other or equal to each other, the central carbon atom can be either the R and S optical stereoisomer or a mixture of R and S stereoisomers, and where AA, BB, and CC are as follows:
AA is A-J with A attached to the carbon atom of the three carbon central unit and J is defined below;

BB is B-Y, with B attached to the carbon atom of the three carbon central unit and Y is hydrogen, alkyl, alkenyl, alkynyl, poly(alkenyl), poly(alkynyl), or poly(alkenoalkynyl) radicals comprised of C1 to C20 carbon atoms; chain lengths or alkanoyl, alkenoyl, alkynoyl. poly(alken)oyl, poly(alkyn)oyl, poly(alkenoalkyn)oyl radicals of C2 to C20 chain lengths, alkyloxy, alkenyloxy, alkynyloxy, poly(alkenyl)oxy, or poly(alkynyl)oxy, poly(alkenoalkynyl)oxy radicals comprised of C1 to C20 carbon atoms;
DD is ; wherein A is oxygen, sulfur, CH2, CF2 or N-R1;
B is oxygen, sulfur, CH2, CF2 or N-R2;
D is oxygen, sulfur, CH2, CF2 or N-R3;
J is a furanose or pyranose radical of the type:

or where X is oxygen, sulfur, CH2, CF2 or N-R4;
F, K, L and M are independently hydrogen, hydroxyl, a protected hydroxyl, alkyloxy, thiol, alkylthio, arylthio, alkylsulfonyl, arylsulfonyl, amino, ammonium, alkylamino, alkylammonium, dialkylamino, dialkylammonium, trialkylamino, trialkylammonium where the alkyl chain on nitrogen is comprised of C1 to C20 carbon atoms; or alkyl, alkenyl, or alkynyl radicals comprised of C1 to C20 carbon atoms;
Z is oxygen or sulfur;
E is oxygen, sulfur, CH2 CF2 or N-R5;
G is alkyl, branched alkyl, cycloalkyl or bridged cycloalkyl radicals of C1 to C20 chain lengths;
Q is halogen, hydroxyl, protected hydroxyl utilizing a protecting group, O-arylsulfonyl-, O-alkylsulfonyl- or O-(perfluoroalkyl)sulfonyloxy, amino, ammonium, alkylamino, alkylammonium, dialkylamino, dialkylammonium, trialkylamino, or trialkylammonium where the alkyl chains on nitrogen are C1 to C20; or Q=NR1R2R3, where R1, R2, or R3 can independently or together be a mixture of alkyl groups of C1 to C20 in chain length and a protecting group and R1 can equal R2, R2 can equal R3, or R1 can equal R3 which can equal R3;
R1, R2, R3, R4 and R5 are independently alkyl, alkeny, alkynyl, branched alkyl, branched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl or cycloalkynyl radicals of C1 to C20 chain lengths, or any protecting group;
and where W1 and W2 are P(-OR), (with R being phenyl, phenylmethyl, or negatively-charged oxygen), S=O, carbon, or sulfur, provided that if W1 is not P(-OR) W2 is P(-OR) and provided that if J is furnose or pyranose radical then W2 if P(-OR).

12. A pharmaceutical composition which is useful in treating a fungal infection according to claim 11, comprising a therapeutically effective amount of an antifungal agent comprising a compound having the structure of:

where R1 is phenyl or phenylmethyl, hydrogen, or nil;
R2 is hydrogen phenylmethyl or any protecting group which can be cleaved by hydrogenolysis;
Q is halogen, hydroxyl, protected hydroxyl utilizing a protecting group, O-arylsulfonyl-, O-alkylsulfonyl- or O-(perfluoroalkyl)sulfonyloxy, amino, ammonium, alkylamino, alkylammonium, dialkylamino, dialkylammonium, trialkylamino, or trialkylammonium where the alkyl chains on nitrogen are C1 to C20; or Q=NR1R2R3, where R1, R2, or R3 can independently or together be a mixture of alkyl groups of C1 to C20 in chain length and a protecting group and R1 can equal R2, R2 can equal R3, or R1 can equal R3 which can equal R3;

13. A pharmaceutical composition which is useful in treating a fungal infection according to claim 11, comprising a therapeutically effective amount of an antifungal agent comprising a compound having the structure of:

where R1 is phenyl, phenylmethyl, hydrogen, or nil;
R2 is hydrogen, phenymethyl or a protecting group cleavable by hydrogenolysis;
R3 is hydrogen or a protecting group;
and Q is a halogen, hydroxyl, O-arylsulfonyl-, O-alkylsulfonyl- or O-(perfluoroalkyl)sulfonyloxy;

14. A pharmaceutical composition which is useful in treating a fungal infection according to claim 11, comprising a therapeutically effective amount of an antifungal agent comprising a compound having the structure of:

, and where R1 is phenyl, phenylmethyl, hydrogen or nil;

R2 is a protecting group, or hydrogen if R1 is not hydrogen;
Q is a halogen, hydroxyl, O-arylsulfonyl-, O-alkylsulfonyl- or O-(perfluoroalkyl) sulfonyloxy.

15. A compound having the structrure of:

where Q is C2 to C30 alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, or branched alkynyl;
Z is oxygen or sulfur; X and Y are independently oxygen, sulfur, CH2, CF2, or N-R1;
A, B, and T are independently alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, or branched alkynyl radicals of C1 to C20 chain lengths; are independently or together cycloalkyl or bridged cycloalkyl radicals of ring size C3 to C20, or cylcoalkenyl or cyclo(polyene) radicals of ring size C4 to C20, cycloalkynyl or cyclo(polyalkynyl)radicals of ring size C8 to C20;
D is oxygen, sulfur, CH2, CF2, or N-R2;
F is alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl or cycloalkynyl radicals containing C1 to C20 carbon atoms;
R1 and R2 are independently hydrogen, alkyl, alkenyl, alkynyl, branched alkyl, branched alkenyl, branched alkynyl, cycloalkyl, bridged cycloalkyl, cycloalkenyl, bridged cycloalkenyl or cycloalkynyl radicals containing C1 to C20 carbon atoms, or a protecting group.

16. A compound having the structure of:

17. A method of synthesizing a phosphocholine derivative comprising the steps of:
(a) phosphorylating an alcohol with a monohaloalkyl containing phosphorylating agent; and (b) displacing the halide of the monohaloalkyl group by an amine to produce the phosphocholine derivative.

18. A method of synthesizing a lysolecithin comprising the steps of:
(a) glycosylating an acetonide derivative of glycerol;
(b) deprotecting the resulting glycerol derivative;
(c) alkylating or esterifying the deprotected product;
(d) phosphorylating the product of step (c) with a monohaloalkyl containing phosphorylating agent; and (e) displacing the halide of the monoalkyl containing phosphate moiety by an amine to form a lysolecithin.

19. The method of claim 18 further comprising:
protecting of one of the hydroxyl functions of the resulting glycerol derivative prior to step (c) and deprotecting the protected hydroxyl group prior to step (d).

20. A method of synthesizing a lysolecithin comprising the steps of:
(a) phosphorylating an acetonide derivative of glycerol;
(b) deprotecting the resulting glycerol derivative;
(c) alkylating or esterifying the deprotected product;
(d) glycosylating the product of step (c) with a monohaloalkyl containing phosphorylating; and (f) displacing the halide of the monoalkyl containing phosphate moeity by an amine to form a lysolecithin.

21. The method according to claim 20 further comprising:
protecting of one of the hydroxyl functions of the resulting glycerol derivative prior to step (c) and deprotecting the protected hydroxyl group prior to step (d).