CA2141206A1 - Insertion de genes par ligation directe in vitro - Google Patents

Insertion de genes par ligation directe in vitro

Info

Publication number
CA2141206A1
CA2141206A1 CA 2141206 CA2141206A CA2141206A1 CA 2141206 A1 CA2141206 A1 CA 2141206A1 CA 2141206 CA2141206 CA 2141206 CA 2141206 A CA2141206 A CA 2141206A CA 2141206 A1 CA2141206 A1 CA 2141206A1
Authority
CA
Canada
Prior art keywords
virus
dna
gene
seq
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2141206
Other languages
English (en)
Inventor
Nancy R. Webb
Peter M. Dierks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2141206A1 publication Critical patent/CA2141206A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de construction de virus d'ADN de recombinaison bicaténaire, en particulier des baculovirus et des virus granulosis, par liaison directe de fragments d'ADN in vitro. L'invention porte également sur des vecteurs viraux de liaison directe qui sont des virus d'insecte modifiés par insertion d'au moins un site de reconnaissance d'une endonucléase de restriction spécifique ne coupant pas le génome viral, de manière à faciliter l'insertion de segments d'ADN étrangers par liaison de l'ADN in vitro, ainsi que sur les virus de recombinaison formés par cette liaison directe. Des vecteurs d'expression modulaires (plasmides) conçus pour faciliter l'assemblage des cassettes d'expression de gènes ou d'autres fragments d'ADN dans des modules d'insertion de virus qui sont insérés ultérieurement dans des vecteurs viraux de liaison directe sur des sites prédéfinis, et selon une orientation prédéfinie par liaison in vitro, ainsi que des virus de recombinaison dérivés desdits vecteurs d'expression modulaires sont également décrits.
CA 2141206 1993-05-28 1994-05-27 Insertion de genes par ligation directe in vitro Abandoned CA2141206A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7016493A 1993-05-28 1993-05-28
US08/070,164 1993-05-28

Publications (1)

Publication Number Publication Date
CA2141206A1 true CA2141206A1 (fr) 1994-12-08

Family

ID=22093545

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2141206 Abandoned CA2141206A1 (fr) 1993-05-28 1994-05-27 Insertion de genes par ligation directe in vitro

Country Status (6)

Country Link
EP (1) EP0658196A4 (fr)
JP (1) JPH07509617A (fr)
AU (1) AU684799B2 (fr)
CA (1) CA2141206A1 (fr)
TW (1) TW502065B (fr)
WO (1) WO1994028114A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0876497A1 (fr) 1995-12-22 1998-11-11 E.I. Du Pont De Nemours And Company Production de baculovirus de recombinaison
US6235278B1 (en) 1996-10-01 2001-05-22 University Of Georgia Research Foundation, Inc. Biological insect control agents expressing insect-specific toxin genes, methods and compositions
WO2000032777A2 (fr) * 1998-12-02 2000-06-08 E.I. Du Pont De Nemours And Company Toxines scorpioniques
EP1185654A2 (fr) 1999-06-22 2002-03-13 E.I. Du Pont De Nemours And Company Toxines du scorpion buthotus judaicus
EP2029157A4 (fr) 2006-05-19 2009-11-18 Georgia Tech Res Inst Ligand des transporteurs abc
CA2727628C (fr) * 2008-06-12 2018-11-27 Expres2Ion Biotechnologies Aps Systeme ameliore d'expression de proteine

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0228036A3 (fr) * 1985-12-18 1988-09-07 Microgenesys, Inc. Méthode de production de polypeptides sélectionnés dans des cellules d'insectes infectées par des virus et polypeptides ainsi isolés
FR2631974B1 (fr) * 1988-05-31 1992-12-11 Agronomique Inst Nat Rech Baculovirus modifie, son procede de preparation et son application en tant que vecteur d'expression de genes
US5155037A (en) * 1989-08-04 1992-10-13 The Texas A&M University System Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
WO1994004699A1 (fr) * 1992-08-26 1994-03-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Ligature in vitro d'adn etranger dans de grands virus eucaryotiques

Also Published As

Publication number Publication date
EP0658196A1 (fr) 1995-06-21
TW502065B (en) 2002-09-11
JPH07509617A (ja) 1995-10-26
AU7048794A (en) 1994-12-20
WO1994028114A1 (fr) 1994-12-08
EP0658196A4 (fr) 1999-07-07
AU684799B2 (en) 1998-01-08

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