CA2132708A1 - Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding - Google Patents

Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding

Info

Publication number
CA2132708A1
CA2132708A1 CA002132708A CA2132708A CA2132708A1 CA 2132708 A1 CA2132708 A1 CA 2132708A1 CA 002132708 A CA002132708 A CA 002132708A CA 2132708 A CA2132708 A CA 2132708A CA 2132708 A1 CA2132708 A1 CA 2132708A1
Authority
CA
Canada
Prior art keywords
aggregation
fluorescent dyes
fluorescence immunoassays
serum binding
dyes free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002132708A
Other languages
French (fr)
Other versions
CA2132708C (en
Inventor
Robert F. Devlin
Walter B. Dandliker
Peter O. G. Arrhenius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Diatron Corp
Original Assignee
Robert F. Devlin
Walter B. Dandliker
Peter O. G. Arrhenius
Diatron Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert F. Devlin, Walter B. Dandliker, Peter O. G. Arrhenius, Diatron Corporation filed Critical Robert F. Devlin
Publication of CA2132708A1 publication Critical patent/CA2132708A1/en
Application granted granted Critical
Publication of CA2132708C publication Critical patent/CA2132708C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0036—Porphyrins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0052—Small organic molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02—Silicon compounds
    • C07F7/08—Compounds having one or more C—Si linkages
    • C07F7/0834—Compounds having one or more O-Si linkage
    • C07F7/0838—Compounds with one or more Si-O-Si sequences
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G01N2333/19—Rubella virus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Fluorescence immunoassays methods are provided which use fluorescent dyes which are free of aggregation and serum binding. Such immunoassay methods are thus, particularly useful for the assay of biological fluids, such as serum, plasma, whole blood and urine.
CA002132708A 1992-03-23 1993-03-23 Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding Expired - Fee Related CA2132708C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85617692A 1992-03-23 1992-03-23
US07/856,176 1992-03-23
PCT/US1993/002470 WO1993019366A1 (en) 1992-03-23 1993-03-23 Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding

Publications (2)

Publication Number Publication Date
CA2132708A1 true CA2132708A1 (en) 1993-09-30
CA2132708C CA2132708C (en) 2005-05-10

Family

ID=25323005

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002132708A Expired - Fee Related CA2132708C (en) 1992-03-23 1993-03-23 Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding

Country Status (3)

Country Link
EP (1) EP0632893A4 (en)
CA (1) CA2132708C (en)
WO (1) WO1993019366A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5919922A (en) * 1990-05-15 1999-07-06 Hyperion, Inc. Fluorescent dyes free of aggregation and serum binding
US6060598A (en) * 1990-05-15 2000-05-09 Hyperion, Inc. Fluorescence immunoassays using fluorescent dyes free of aggregation and serum binding
US5880287A (en) * 1990-05-15 1999-03-09 Hyperion, Inc. Polyoxyhydrocarbyl related products and methods for fluorescence assays
US7322927B2 (en) 1993-09-24 2008-01-29 Biosite, Inc. Hybrid phthalocyanine derivatives and their uses
US7083984B2 (en) 1993-09-24 2006-08-01 Biosite, Inc. Hybrid phthalocyanine derivatives and their uses
US6238931B1 (en) 1993-09-24 2001-05-29 Biosite Diagnostics, Inc. Fluorescence energy transfer in particles
US5824799A (en) * 1993-09-24 1998-10-20 Biosite Diagnostics Incorporated Hybrid phthalocyanine derivatives and their uses
EP0820489B1 (en) * 1995-03-23 2001-07-11 Biosite Diagnostics Inc. Hybrid phthalocyanine derivatives and their uses
US5876935A (en) * 1997-01-08 1999-03-02 Dade Behring Inc. Luminescent specific binding assay
CA2350608A1 (en) * 1998-11-25 2000-06-02 Hyperion, Inc. Water soluble fluorescent dyes free of aggregation and serum binding and related products and methods
EP1780213A1 (en) 2005-10-27 2007-05-02 3M Innovative Properties Company Silicon-urea-azolides, their preparation and use in the preparation of silicones with isocyanate terminal groups
WO2010019967A1 (en) * 2008-08-15 2010-02-18 Georgetown University Fluorescent cdk inhibitors for treatment of cancer

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0645681B2 (en) * 1986-02-05 1994-06-15 美浜 久春 Modified hem
ATE98020T1 (en) * 1986-12-15 1993-12-15 British Tech Group Usa MONOMERIC PHTHALOCYANINE REAGENTS.
EP0529002B1 (en) * 1990-05-15 2001-10-04 Hyperion, Inc. Fluorescent porphyrin, and fluorescent phthalocyanine - polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes
CA2082934C (en) * 1990-05-15 2003-08-05 Walter B. Dandliker Phthalocyanatopolyethylene glycol, and phthalocyanato saccharides as fluorescent digoxin reagents
EP0502723B1 (en) * 1991-03-05 1996-10-09 Hitachi Chemical Co., Ltd. Water-soluble tetraazaporphins and fluorochrome for labeling

Also Published As

Publication number Publication date
CA2132708C (en) 2005-05-10
EP0632893A4 (en) 1997-01-02
EP0632893A1 (en) 1995-01-11
WO1993019366A1 (en) 1993-09-30

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Legal Events

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