CA2108533A1 - Monoclonal antibodies directed against ndp-kinase - Google Patents

Monoclonal antibodies directed against ndp-kinase

Info

Publication number
CA2108533A1
CA2108533A1 CA 2108533 CA2108533A CA2108533A1 CA 2108533 A1 CA2108533 A1 CA 2108533A1 CA 2108533 CA2108533 CA 2108533 CA 2108533 A CA2108533 A CA 2108533A CA 2108533 A1 CA2108533 A1 CA 2108533A1
Authority
CA
Canada
Prior art keywords
kinase
ndp
mab
cells
monoclonal antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2108533
Other languages
French (fr)
Other versions
CA2108533C (en
Inventor
Gabriel Pulido-Cejudo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canada Minister of Health
Original Assignee
Gabriel Pulido-Cejudo
Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Health
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gabriel Pulido-Cejudo, Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Health filed Critical Gabriel Pulido-Cejudo
Publication of CA2108533A1 publication Critical patent/CA2108533A1/en
Application granted granted Critical
Publication of CA2108533C publication Critical patent/CA2108533C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A monoclonal antibody against cytosolic nucleoside diphosphate kinase (NDP-kinase: EC 2.7.4.6) from HL60 cells has been produced. The monoclonal antibody solely recognizes the active oligomeric form of NDP-kinase and does not inhibit NDP-kinase activity. Reduction and alkylation of NDP-kinase oligomer performed under denaturing conditions fully destroyed the antigenic site recognized by the monoclonal antibody.
Western blot analysis after non-denaturing polyacrylamide gel electrophoresis (PAGE) revealed that a single protein oligomer is recognized by the MAb. The protein band showed the same electrophoretic mobility of pure cytosolic NDP-kinase detected by a specific NDP-kinase enzymic staining assay performed after nondenaturing PAGE. Collectively, the results suggest that the MAb recognizes the native form of NDP-kinase.
Searching for the reactivity of the MAb against membrane-bound NDP-kinase it was found that the MAb selectively reacts with a cell-membrane component of monocytes (93%), granulocytes (97%) and 14% of lymphocytes. Two color immunofluorescence showed that the MAb reacted only with CD19+ B cells (93% of CD19+ B
cells), which suggest that NDP-kinase may play an important role in normal B lymphocyte function.
CA 2108533 1992-10-16 1993-10-15 Monoclonal antibodies directed against ndp-kinase Expired - Fee Related CA2108533C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96233892A 1992-10-16 1992-10-16
US07/962,338 1992-10-16

Publications (2)

Publication Number Publication Date
CA2108533A1 true CA2108533A1 (en) 1994-04-17
CA2108533C CA2108533C (en) 2002-10-08

Family

ID=25505718

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2108533 Expired - Fee Related CA2108533C (en) 1992-10-16 1993-10-15 Monoclonal antibodies directed against ndp-kinase

Country Status (1)

Country Link
CA (1) CA2108533C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000060357A1 (en) * 1999-03-30 2000-10-12 Canbreal Therodiagnostics Canadian Holdging Corporation Tandem immuno-assay for cancer
CN110596367A (en) * 2019-09-06 2019-12-20 广州科方生物技术股份有限公司 Stabilizer capable of prolonging effective period of brain natriuretic peptide calibrator and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000060357A1 (en) * 1999-03-30 2000-10-12 Canbreal Therodiagnostics Canadian Holdging Corporation Tandem immuno-assay for cancer
US6521415B1 (en) 1999-03-30 2003-02-18 Canbreal Therodiagnostics Canada Holding Corporation Tandem immuno-assay for cancer
CN110596367A (en) * 2019-09-06 2019-12-20 广州科方生物技术股份有限公司 Stabilizer capable of prolonging effective period of brain natriuretic peptide calibrator and preparation method thereof

Also Published As

Publication number Publication date
CA2108533C (en) 2002-10-08

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