CA2108533A1 - Monoclonal antibodies directed against ndp-kinase - Google Patents
Monoclonal antibodies directed against ndp-kinaseInfo
- Publication number
- CA2108533A1 CA2108533A1 CA 2108533 CA2108533A CA2108533A1 CA 2108533 A1 CA2108533 A1 CA 2108533A1 CA 2108533 CA2108533 CA 2108533 CA 2108533 A CA2108533 A CA 2108533A CA 2108533 A1 CA2108533 A1 CA 2108533A1
- Authority
- CA
- Canada
- Prior art keywords
- kinase
- ndp
- mab
- cells
- monoclonal antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A monoclonal antibody against cytosolic nucleoside diphosphate kinase (NDP-kinase: EC 2.7.4.6) from HL60 cells has been produced. The monoclonal antibody solely recognizes the active oligomeric form of NDP-kinase and does not inhibit NDP-kinase activity. Reduction and alkylation of NDP-kinase oligomer performed under denaturing conditions fully destroyed the antigenic site recognized by the monoclonal antibody.
Western blot analysis after non-denaturing polyacrylamide gel electrophoresis (PAGE) revealed that a single protein oligomer is recognized by the MAb. The protein band showed the same electrophoretic mobility of pure cytosolic NDP-kinase detected by a specific NDP-kinase enzymic staining assay performed after nondenaturing PAGE. Collectively, the results suggest that the MAb recognizes the native form of NDP-kinase.
Searching for the reactivity of the MAb against membrane-bound NDP-kinase it was found that the MAb selectively reacts with a cell-membrane component of monocytes (93%), granulocytes (97%) and 14% of lymphocytes. Two color immunofluorescence showed that the MAb reacted only with CD19+ B cells (93% of CD19+ B
cells), which suggest that NDP-kinase may play an important role in normal B lymphocyte function.
Western blot analysis after non-denaturing polyacrylamide gel electrophoresis (PAGE) revealed that a single protein oligomer is recognized by the MAb. The protein band showed the same electrophoretic mobility of pure cytosolic NDP-kinase detected by a specific NDP-kinase enzymic staining assay performed after nondenaturing PAGE. Collectively, the results suggest that the MAb recognizes the native form of NDP-kinase.
Searching for the reactivity of the MAb against membrane-bound NDP-kinase it was found that the MAb selectively reacts with a cell-membrane component of monocytes (93%), granulocytes (97%) and 14% of lymphocytes. Two color immunofluorescence showed that the MAb reacted only with CD19+ B cells (93% of CD19+ B
cells), which suggest that NDP-kinase may play an important role in normal B lymphocyte function.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US96233892A | 1992-10-16 | 1992-10-16 | |
US07/962,338 | 1992-10-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2108533A1 true CA2108533A1 (en) | 1994-04-17 |
CA2108533C CA2108533C (en) | 2002-10-08 |
Family
ID=25505718
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2108533 Expired - Fee Related CA2108533C (en) | 1992-10-16 | 1993-10-15 | Monoclonal antibodies directed against ndp-kinase |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2108533C (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000060357A1 (en) * | 1999-03-30 | 2000-10-12 | Canbreal Therodiagnostics Canadian Holdging Corporation | Tandem immuno-assay for cancer |
CN110596367A (en) * | 2019-09-06 | 2019-12-20 | 广州科方生物技术股份有限公司 | Stabilizer capable of prolonging effective period of brain natriuretic peptide calibrator and preparation method thereof |
-
1993
- 1993-10-15 CA CA 2108533 patent/CA2108533C/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000060357A1 (en) * | 1999-03-30 | 2000-10-12 | Canbreal Therodiagnostics Canadian Holdging Corporation | Tandem immuno-assay for cancer |
US6521415B1 (en) | 1999-03-30 | 2003-02-18 | Canbreal Therodiagnostics Canada Holding Corporation | Tandem immuno-assay for cancer |
CN110596367A (en) * | 2019-09-06 | 2019-12-20 | 广州科方生物技术股份有限公司 | Stabilizer capable of prolonging effective period of brain natriuretic peptide calibrator and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
CA2108533C (en) | 2002-10-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |