CA2100583A1 - Polymorphic dna markers in bovidae - Google Patents

Abstract

2100583 9213102 PCTABScor01 Described is a collection of bovine genomic clones that map to polymorphic loci in bovids. Said clones will find utility in genetic identification, gene mapping and selective breeding.

Description

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~0 92/13102 PCr/l,'S92/00340 TITLE OF THE INYENTION
POLYMORPHIC DNA MAR~RS IN BOVIDAE

CROSS-REFERENCE TO RE:r!~TED_APPI~Cal`IONS

The present invention is a continuation-in-part of U.S. Ser. No. 642,342, filed January 15, 1991, incorpo-rated herein by referenc~.

FIE~D OF THE INVENTION

The invention relates to gene mapping, selective breeding and genetic identification in domestic animals.

1~ BACKGROUN~ OF THE I~VENTION

The publications and other materials used herein to illuminate the background of the invention, and in particular, cases to provide additional details respect-ing the practice, are incorporated by reference and for convenience are numerically referenced in the following text and respectively grouped in the appended bibliog-raphy.
Until recently, artificial selection has relied on the biometrical evaluation of individual breeding values from an animal's own performance and from performance of its relatives (136). This biometrical strategy is based on relatively simple genetic premises, operating within a "black box". Briefly, the majority of economically important traits are so-called complex or quantitative traits, meaning that the phenotype of an animal is determined by both environment and a large number of genes with individually small, additive effects. The proportion of the phenotypic variation observed in a given population that is genetic in nature is the heritability of the trait. Substantial genetic progress has been obtained using this approach. One of the 2100~3 WO92~13102 PCT/~'S92~00340 powers of this biometrical approach is that it obviates the need for any detailed molecular knowledge of the underlying genes or Economic Trait Loci.
However, it is believed that the molecular identification of thase Economic Trait Loci could increase genetic response by affecting both time and accuracy of selection, ~hrough a proc@dure called Marker Assisted Selection (9l, 96). One strategy towards the i~olation of Economic Trait Loci relies on the use of DNA Sequence Polymorphisms as genetic markers in linkage studies. This approach, paradoxically referred to as "Reverse Genetics" (138), will be described in detail in this introduction. Moreover, we propose a new concept called "Velogenetics", or the combined use of Marker Assi~ted Introgres~ion and germ-line manipulations to shorten the generation interval of domPstic sp~cies (especially cattle), which will allow the rapid and efficient introgression of mapped Economic Trai~ Loci between genetic backgrounds.

I. DNA SEQUENCE POLYMORPHISM (DSP) A. Tyes of DNA Seauence PolYmorDhism The typical mammalian genome is composed of an approximately 3xl09 base pairs long DNA stretch, divided over a species-specific number of chromosomes, and cont~ining all the information required for the proper development and functioning of a normal being. Each individual has two copies of this message: one paternal in origin and one maternal. Although their overall architacture and cont~nt are virtually identical, the paternal and maternàl DNA sequences exhibit subtle "allelic" di~ferences, hereinafter referred to as DNA
Sequence Polymorphisms or "DSP". The DSP that can be recognized in a given population are the molecular basis of the genetic component of the observed phenotypic variance. One can distinguish three types of DSP.

WO92/13102 2 1 ~ O S ~ 3 pcT/uss2/oo~o 1. Sinale Base Palr Polvmorphisms As their name implies, these DSP are due to single bas~ pair differences distinguishing alleles. These can be either base pair substitutions o transitions (Purine to Purine or Pyrimidine to Pyrimidine) and transversions (Purine to Pyrimidine and vice versa) -, or the inser-tion/deletion of a single base pair.
The frequency of single base pair polymorphism is measured by the nucleotide diversity~ ~, or average heterozygosity per nucleotide site ~ he nucleotide diversity has been estimated from Restriction Fragment Length Polymorphisms at 0.002 for human (2), and at 0.0007 in càttle (3,4). This means that on the average a human will be heterozygous for one every 500 nucleo-tides, and a cow for one every 1,500 nuclaotides.
One type of single base pair polymorphism deserves special attention: the CpG to TpG transition. The cytosine in the CpG dinucleotide sequence is known to be the substrate of an eucaryotic methylase, which will add a methyl group in position 5 of the pyrimidine ring, if the cytosine of the complementary CpG dinucleotide is itself methylated. Deamination of a 5-methylcytosine generates a thymine, blurring the task of the DNA repair machinery which will half of the time resolve the ensuing mismatch by replacing the original guanine instead o~ the mutated thymine. As a consequence, cyto-sines in the CpG doublet exhibit mutation rates at least ten times higher than other nucleotides, and hence are ! Irich sources of single base pair polymorphisms (4, 5).

2. ~NA Se~uence Rearranaements In this kind of DSP, the difference between allelic variants involves DNA sequence rearrangements such as the insertion or deletion of a stretch of DNA, DNA
sequence inversions and duplications.
Although there is a wide spectrum of molecular mechanisms susceptible to generate such chromosomal re-2 1 1) (1 ~

arrangements, it is well established that mobile genetic - elements significantly contribute to this kind of DSP.
In lower eukaryotes such as Drosophila and yeast, rearrangements involving transposable elements account for a large proportion of new mutations detected in these organisms (6). In the mouse, retrovirus like sequences or retrotransposons have been shown to act as insertional mutagens (7-ll), and different strains of mice exhibit substantial heterogeneity with respect to the numbers and chromosomal sites of endogenous pro-viruses (12). Variation in the distribution of endoge-nous retroviruses has been demonstrated in poult:ry as well.
In the human, at least 10% of the genome is known to be composed of retroposon-like sequences. Evidence for a role of these sequences in human genetic varia-bility and disease stems from several reports of de novo mutations due to these sequ~nces: a mutation in the human Low Density Lipoprotein receptor gene giving rise to familial hypercholesterolemia is caused by a deletion brought about by an intrastrand recombination event between two Alu sequences (13); Ll insertions were found to inactivate the ~actor VIII gene in hemophilia A
patients (14); a c-myc rearrangement in a breast carcin-oma was found to be due to insertion of an Ll element (15); an Alu transposition event has been documented in human lung carcinoma cells (16); and an homologous recombination between the LTRs of a human retrovirus-like element was shown to cause a 5 Kb deletion poly-morphism. Recently, Wong et al. (17) reported evidence of human DNA polymorphism arising through DNA-mediated, rather than RNA-mediated, transfer between autosomes a~
well.

3. Exansion-Contraction TYpe Polymorphism 3s A significant proportion of the eucaryotic genome is composed of sequences widely termed "satellite DNA,"

21~3~3 ~092J13102 PCT/~'S92/00340 sharing a common organization: a sequence motif, varying in length between one and several thousand nucleotides, repeated in a head-to-tail or so-called tandem arrange-ment. Depending or. the methodology originally used for their study, iOe. isopycnic centrifugation, pulsed field gel electrophoresis, agarose gel electrophoresis or polyacrylamide gel electrophoresis, satellite se~uences were grouped into four size olasses: macro-, midi-, mini- and micro-satellites. Minisatellites are also known as Variable Number of Tandem Repeats (~NTRs) (18-21). While macro-satellites seem to be confined to hetarochromatic regions (22~, mini- and micro-satellites ha~e been found scattered throughout the genome with, however, clustering of mini-satellites (23-34). In the human, minisatellite clusters seems to be particularly abundant in proterminal regions (35). The only midi-satellite described as such today, has been mapped to the short arm of chromosome 1 (36)~ In the human, the polydaoxyadenylate tract o~ Alu repetitive elements are 29 also caracterized by length variation and are thus an abundant source of genetic markers as well (37).
The ~unction, if any, of satellite sequences, whether macro-, midi-, min~- or micro-, is essentially unknown. An important feature of all satellite sequences is that the maintenance of their tandemly repeated organization is dependent on the concerted evolution of the repeatsq This concerted evolution is thought to xesult from subsequent rounds of unequal crossing-over ;(or any other mechanisms fitting the "card deck" model (38)), which are favored ~y the tandemly repeated structure itself. The proposed unequal crossing-over mechanism, whether happening between sister chromatids or homologous chromosomes, explains the substantial degree of length polymorphism, here referred to as 3~ "expansion-contraction polymorphism," characterizing those sequences. Moreover, the ensuing shuffling of slightly divergent repeat units or Minisatellite Variant 2 1 00 S 8 3 ~
WO92/1310~ PCT/~'S92!00340 Repeats (39) within the satellite generat~s additional internal site polymorphism. These peculiar properties of satellite sequences have made them an invaluable source of highly informative genetic markers, both in the human and in domestic species ~reviewed in 31).

B. Detection of DNA Sequence Polvmorphism During the last ten years, a multitude of methods have been developed for the detection of DSP. Two techniques, however, undoubtedly dominate this field:
Southern blot hybridization (40) and the Polymerase Chain Reaction or PCR ~41), used either separately or in conjunction. A non-exhaustive list is reported here, the methods being grouped into four classes.

1. Restriction Pattern Analysis DSP may alter the restriction patterns of defined chromosomal regions, generating so-called Restriction Fragment Length Polymorphisms (RFLP). Depending on the size-range of the explored restriction fragments t one will use either agarose gel electrophoresis, pulse-field ~0 gel electrophoresis t30) or polyacrylamide gel electrophoresis (42) for intermediate, large or small fragments respectively. RFLPs are classically detected by Southern blot hybridization. Al~ernatively, one can analyze restriction patterns of defined DNA sequences amplified by PCR, generating so-called Amplified Sequence Polymorphisms (43). When studying chromosomal r,earrangements or expansion-contraction type polymorph-isms, the use of PCR obviates the need for restriction enzyme digestion, the DSP reflecting itself in the size of the amplified product.
Because of its simplicity, the detection of RFLPs has by far been the most popular approach towards DSP.
The relative lack of power inherent to the method (only 20% of a given sequence is amenable to exploration using the most common restriction enzymes) can be compensated 2 1 ~ ~ ~ 8 3 WO92/13102 PCT/~'S92/00340 for by focusing on highly polymorphic seguences such as CpG dinucleotides (using enzymes such as ~3~I and Ms~I
containing CpG in their recognition sequence) or hyper-variable minisatellites. The discovery, however, of microsatPllites as a very abundant source of highly informati~e DSP in a broad taxonomic range, easily detectable by PCR, is likely to shift the focus towards these seguences for future marker development (32 34, 37, 44).

2. Mismatch Analysis Several methods for the detection of DSP are based on the study of mismatch analysis. DNA to analyze is probed with a ~e~uence corresponding to a defined genetic variant~ The presence of a different variant in the target DNA generates a mismatched heteroduplex, which can be detected by various means:

a. Detection of Altered Meltinq Behavior A mismatched heteroduplex will differentiate itself from the perfectly matched homoduplex by an altered melting behaYior which can be detected as an all-or-none, binary response: positive for the homoduplex, negative for the heteroduplex, or in a more graded response, allowing to distinguish between different heteroduplex variants.
The classical all-or-none test depends on the use of allele-specific oligonucleotidec in hybridization ~xperiments. By choosing appropriate hybridization and washing conditions, the allele-specific oligonucleotide will only recognize a perfectly complementary sequence (45). With the advent of PCR, new variants of this approach have been described including reverse dot-blot (46), the Amplification Refractory Mutation System (47) or allele-specific polymerase chain reaction (48), and Competitive Oligonucleotide Priming (49). The Ligation Amplification Reaction, amplification of specific DNA

2100~3 -WO92/13102 PCT/~S~2~0~0 sequences using sequential rounds of template-dependent ligation, can also be considered as a peculiar applica tion of the allele specific oligonucleotide approach (50).
More discriminating is Denaturing Gradient Gel Elec-trophoresis, exploring the pattern of melting behavlor characterizing each heteroduplex when electrophoresed through an increasing gradient of DNA denaturants (51).
The sensitivity of this method can also be improved by pre-amplifying the target sequence by PCR.

b. Ribonuclease and chemical mismatch detection The presence of a mismatch in a heteroduplex makes those molecules susceptible to cleavage by variouc means including chemical treatment w~th either hydroxylamine or osmium tetroxide (52), as well as ribonucleases such as RNase A in case of an RNA:DNA hetaroduplex (51).
Electrophoretic analysis of the cleavage products allows one to distinguish different ~enetic variants. Again, implementing PCR will increase the sensitivity of the approach.

3. Sinqle Stranded Conformation Polymorphism Under nondenaturing conditions, single-stranded DNA
has a folded conformation that is stabilized by intra-strand interactions. Consequently, the conformation, and therefore the electrophoretic mobility, is dependent on the sequence. DNA ~ariants exhibit indeed mobility shifts when electrophoresed in such conditions, presum-ably resulting from conformational changes caused by sequence alterations, hence the name single stranded conformation polymorphism. Again, the altered mobility can be detected by blot hybridization analysis or rely-ing on PCR (53, 54~.

2 1 ~ 3 ~092/13102 PCT/USs2/00340 _g _ 4. Direct Determinati n of the DNA se~ence Obviously the most powerful approach towards DSP is the direct determination of the DNA sequence. The need of a cloning step, however, in classical sequencing pro-tocols precluded ~he analysis of large samples. This limitation has been circumvented by the development of genomic sequencing (55), allowing the direct determina-tion of defined DNA sequences from genomic DNA, an~ more recently and le55 laboriously by the development of direct sequence determination of PCR amplified products.
The feasibility of the latter approach for the detection of DSP has been amply demonstrated in several inde!pend-ent studies (~ee, for instance, 563.

C. riain and Evolution of DNA Sequence Poly~orphism DSP encountered in a given population find their origin in mutational events occurring in the germline and escaping the DNA repair machinery. The fate of these germline mutations in the population is dominated by two kinds of effects: stochastic and deterministic effects.

l. $tochastic Effects When a new mutation-appears in the population, its initial survival depends largely on chance, regardless of its selective effect. This is easily illustrated as follows. Assume an individual heterozygous for a neo-mutation inherited from its parent, in whose germline the mutation appeared. If this individual has one, two or three offspring, the chances for the neomutation to be lost from the population, because transmitted to none of the offspring, are 0.5, 0.25 and 0.125 respectively.
Even if inherited by part of the o~fspring, the same "stochastic filter" will operate in the next generation.
In the course of this random drift, the overwhelming majority of mutant alleles are lost by chance. However, some will see their frequency increase in the 2100S23 ~
WO92/13102 ~CT/US9~/0034~

--lOo population, and despite fluctuations over time, - eventually become fixed in the population, until substituted by the next mutant allele.
As demonstrated essentially by Kimura (57) in the framework of his neutral theory of molecular evolution, the probability for a selectively neutral neomutation to be fixed in a population of N individuals is equal to its initial fre~uency l/2N, the average time for fixation is four tim s the "effective" population size or 4Ne, and the rate k of mutant substitution per generation is simply equal to the rate of mutation per gamete and per generation, ~, independent of what the population size may be.
According to this view, a polymorphism observed in a population at a given time is composed of "transient"
alleles catched in their stochastic "odyssey" throughout the population.
Populations for which 4.Na.~ ~ 1 are essentially monomorphic, while populations for which 4.Ne.~ ~ 1 are characterized by a substantial degree of transient polymorphism. The model predicts a steady state level of heterozygosity, H:
4.Ne.
H ~
4.Ne.~ + 1 2. Determlnistic Effects There is evidence that the fate of a significant proportion of DSP, especially those occurring in non-coding parts of the genome (composing the large majority of the genome), is essentially dominated by random drift. However, when a neo-mutation affects a DNA
sequence which is expressed at the phenotypic level in the broad sense, the mutation may not longer be selec-tively neutral, and deterministic effects will be superimposed on the stochastic ones. Negative and positive selection will respectively decrease or O~ 31o~ 2 1 0 ~ 5 ~ 3 PCT/~'S92/0~0 ~11--increase the probability and rate of fixation, while "balancing selection" will maintain specific alleles in a population in an equilibrium state.

a. Neqative Select~on When comparing DNA sequences between taxa, it appears that the estimated number of mutant substi-tutiQns per nucleotide to account for the observed divergence is highest for non-coding sequences, such as pseudogenes and intronic sequences, and much lower for coding sequences. For the latter, however~ a diffe.rence must be made between first, second and third posi.tions of the codons. The third position, for which only 28~
of substitutions are expected to cause an amino acid change (versus g5% and 100% for first and second positions respectively), exhibi~s the highest substitu-tion rate. ~hen estimating that part of substitutions at the third positions which are so-called synonymous, rates very similar to non-coding r2gions are observed (57). Moreover, DSP are more prominent for non-coding sequenceR and, within coding sequences, at third codon positions (compared to first and se~ond positions (58)).
These observations are easily explained by assuming that the fate of neomutations arising in noncoding regions or of synonymous neomutations, is dominated by stochas-tic effects, ~hile the fate of mutations causing amino acid replacements will depend as well on whether or not they disrupt the function of the protein, in which case they will be eliminated from the population by negative, "purifying" selection. The higher the functional con-straints imposed on a protein, the higher the proportion of neomutations expected to be harmful and, hence, the lower the substitution rate, expressed at the protein level as a higher "unit evolutionary time" (average time required for one amino acid change to appear in a sequence of 100 amino acid residues).

210058~

These ob~ervations have been considered as a strong - argument in favor of a predominant role for random drift in the dynamics of molecular evolution.

b. Positive sel ction According to the previous discussion, the major drive behind molecular evolution is non-adaptive in nature, which is in conflict with the classical theory of daptive, positive Darwinian selection.
There i5, however, evidence for positive and adaptive evolution at the molecular level in at least a few instances. comparing DNA sequences from members of two gene families: the serine protease inhibitors in rat (59~ and the pregnancy specific B~ glycoprotein gene family in man, evidence has been found for higher substitution rates at first and second codon positions than at the third position, in at least some protein regions, pointing towards positive selection.
Moreover, there are a number of experimental data suggesting that some allelic differences identified by electrophoresis are associated with adaptation to different environments. In Drosophila, for instanre, their is evidence for correlation between n vitro heat resistance of ADH variants and the temperature charac-terizing their geographical origin (58).

c. Balancina selection The evolutionary forces described so far generate transient DSP in the sense that the population fre-quencies of existing genetic variants will irrevocably change with time until either fixation or los~. In some cases, however, alleles may be maintsined in a popula-tion at a steady state level. Overdominance is one of the mechanisms susceptible to generate such a "balanced polymorphism". For a two allele system, this means that the heterozygous individuals benefit from a selective advantage compared to both homozygous genotypes. This 21~0~3 WO92/1310~ PCT/U~92/00340 -~3-is expected t~ generate a steady state where both allel~s are maintained in the population at respective equilibrium frequencies p and q, where q ~ ~ and p - -s + t s + ~

s and t ~eing the respective selection coefficients of the homozygotes.
The best known example of balanced polymorphilsm due to overdominance is the maintenance of the S ~-globin allele (causing sickle-cell anemia in the homozygotes) as well as thalassemia-causing mutants (see" for instance, ~l) in populations subjected to ma:laria, because of the resistance exhibited by the heterozygotes towards the parasite. The high level of polymorphism observed at the Major Histocompatibility Locus is thought to result from overdominance selection as well (62).
Frequency depend2nt selaction may be another cause of balanced polymorphism, an exampl~ being the "rare mate advantage" observed in Drosophila (63).

II. CONSTR~CTION OF PRIMARY DNA MARXER MAPS

A. Linkaae Strateqies Two loci ~re said to be genetically linked if, during meiosis, they recombine at significantly lower than a 50% rate, i.e., they generate significantly more parental gametes than recombinant gametes. The recom-bination rate between loci reflects the frequency of occurrence of an uneven number of crossing-overs between the loci. Because the probability for crossing-over is proportional to the distance separating the loci, the recombination rate can be used as a unit of chromosomal length. This length unit is known as the Morgan (M), W092/1310' 2 1 9 oi 5 8 3 PCT/US92/00340 l cM corresponding to the distance separating two loci exhibiting a 1% recombination rate. For small distance (~30cM), the relation between centimorgan and recombin-ation rate is essentially linear. For longer distances, however, the relation is more complex, depending on the frequency of double crossing-overs, itself affected by eventual interference.
Parental and recombinant gametes will only be distinguishable for doubly heterozygous individuals, hence the need for highly polymorphic markers.
Recently, and due to the advent of the PCR, it has been possible to directly determine the genotype of individual gametes (64). However, most of the time, the gametic contribution is inferred from the genoty~e of the offspring and linkage studies are performed within families. Most modern linkage studies use the lodscore test for evaluation of linkage: a sequential.test based on the method of maximum likelihood (65). The lodscore corresponds to loglO(LR), where LR corresponds to the ratio: likelihood of observations under alternative hypothesis eso .5, divided by the likelihood of observ-ations under null hypothesis of no linkage, e=o .5. In human genetics, a lodscore > 3 is accepted as signif-icant evidence for linkage. The prior probability of linkage between two loci has been used to justify this stringent critical value. Note that 21n(LR) can be used as well, having a chi-square distribution with one degree-of-freedom under the null hypothesis of no linkage.
Recently, algorithms for multilocus linkage analy-sis have been developed, allowing an estimate of the most likely gene orders and genetic distances between several loci simultaneously (66-68).
Although usually determined within families, genetic linkage can manifest itself at the population level also: a phenomenon called "linkage disequilib-rium". According to the Hardy-Weinberg law, the S ~ 3 WO 92/1310~ PCr/l,'S9~/00340 equilibrium genotypic frequencies are reached in a single generation (except if t~e initial gene frequen-cies are not equal among sexes). For a diallelic system with alleles al and a2, with respective allelic frPquen-cies pl and p2, the equilibrium genotypic frequencies are pl2, 2plp2 and p22 for alal, a1a2 and a2a2 respec-tively. This does not necessarily hold when considerins two loci si~ultaneously. The genotypic equili~rium frequencies are only reached when the previous genera-tion produces the four possible gametes at the expected frequencies: albl: plql, a2bl: p2~1, albl: p2~1, a2b2:
p2q2. The difference between observed and e~pected gametic frequencies is c~lled linkage disequilibriu~, D.
The value of D is reduced by d. e every generation, e being the recombination rate between the two loci. For unlinked loci D diminishes by 1/2 every generation; for linked loci, however, the reduction of D per generation will be much smaller. The detection of a linkage disequilibrium is an indication of linkage between the correspondinq loci.

B. Genetic Mas Using this linkage approach, combined with alterna-tive mapping strategies such as "in situ" hybridization (see, for instance, 69), the use of somatic cell hybrid panels and radiation hybrid mapping (reviewed in 70) and comparative mapping (71), the map location of a large set of DSP can be determined in order to build a genetic marker map (see, for instance, 72-74). Assuming a total map length of 30M as for the human, and a desirable maximum distance of 20cM between markers, a set of 150 DSP could cover the entire genome. However, many more markers will be needed to generate reasonable maps for our domestic species, es~entially for two reasons.
First, most of the time we have no a priori information on the location of the characterized markers. Hence, some chromosomal regions will initially be 2 1 0 0 ~ ~ 3 WO92/13102 PCT/~IS92/0034t) overrepresented in our map, others underrepresented.
This problem is expected to become critical in the later stages of the development of a map. Comparative data will then become critical, allowing to search for markers whose location can be predicted from other species. Second, an individual will only be informative for the markers for which he is heterozygous; parts of his genome will thus not be explorable, because he will be homozygous for the corresponding mar~ers. To compen-sate for this, one will have to identify more markers, the number required being inversely proportional to their heterozygosity -- hence, the importance of highly informative systems.
Once such a map is available, however, any gene for which the appropriate segregating family material is available can be located on the map. Assuming a maximum marker-target gene distance of l0cM, the expected lodscore for doubly informative, phase-known meioses approximates 0.16 (75). Therefore, 20 such meioses are theoretically sufficient to establish linkage with a lodscore of 3. In practice, howe~er, the number of individuals to analyze will be higher, a function among other factors of the quality of the marker; expressed as its Polymorphism Information Content (76).
The efficiency of this approach has been illus-trated by the recent mapping of a large number of genes involved in human single gene disorders (see, for instance, 77, 135). The identification of DNA markers for a defined gene can be the first step towards its molecular cloning. Successful "positional cloning", or the isolation of a gene based on its map location, has been achieved in the human for Chronic Granulomatous Disease, Duchennes Muscular Dystrophy, Retinoblastoma, Wilms Tumor, Cystic Fibrosis (134), Type-l Neurofibroma-tosis and the Testis Determining Factor.
In domestic animals, genetic maps could be used to localize the genes underlying production traits, W0~2/13l0~ PCT/USg~/0034 allowing for Marker ~ssisted Selec~ion~ and a first step towards their isolation, the understanding of their mechanism of action and their manipulation by mutagene-sis and gene transfer methods. Several laboratories around the world are now involved in the development of markers and the construction of qenetic maps for our main domestic species, especially cattle, pigs and poultry.

III. GENETIC MAPPING OF QUANTITATIVE TRAIT LOCI

The majority of traits de~lt with in anima]L pro-duction are so-called quantitative traits, chara~erized by continuous variation. The phenotype of an animal with respect to a particular trait is the result of the effect of a several "polygenes" known as Quantitative Trait Loci, or QTL, combined with nvironmental effects.
The number of polygenes involved is essentially unknown.
Classically, it is considered very large, each gene contributing a very small part of the genetic variation.
However, there is evidence both from the plant world and the animal world, that QTL with significant effects are common (78, 79). The most likely model is to assume that there is indeed a large number of genes involved, but that there is a broad distribution of effects, substan-tial in some cases. Polygenes with extreme effects, whose segregation in a population may cause skewness and bi- or trimodality, are known as "major genes". Examples in animal breeding are "double muscling" qenes in both cattle and pigs, the "White Shorthorn" gene involved in the determinism of "White Heifer Disease" and the "Booroola" fertility gene in sheep (80). Even with significant effects on the trait of interest, however, their contribution to the total genetic variation may be limited in case of low population frequency.
When dealing with quantitative traits, direct determination of genotype for the corresponding QTL is 21~05~3 ~ .
W092/~3l02 PCT/US92/00340 impossible. Nevertheless, strategies have been designed 3 to map QTL by linkage analysis. Within segregating populations, which is ~sually the case for our domestic species, QTL mapping can be performed both within families and at the population level.

A. OTL Ma~ina Within Families Traditionally one proceeds as follows: offspring from an indi~idual heter~zygous for both marker and QTL
are grouped according to which allele at the marker locus they inherited; a statistically significant difference between the phenotypic means of the~ two groups indicates linkage between marker and QTL. Test for statistical significance is done by linear regres-sion (i.e. one-way analysis of variance) under the assumption of normally-distributed residual environ-mental variance. Classically, markers are tested one at a time for possible linkage with a QTL affecting the trait of interest. One of the drawbacks of this approach is that it is impossible to unequivocally estimate both map location of the QTL with respect of the marker, and its effect on the considered trait; no distinction can be made between a closely linked QTL with small effect and a loosely linked QTL with major effect.
Recently, the lodscore method has been improved, making it possible to deal with quantitative and other complex traits and fully exploiting the power of the nearly complete marker maps which ha~e become available ~or different organisms. This approach is known as interval mapping. Not only does interval mapping solve the problem of simultaneous estimation of location and effect, but because of its increased power, it reduces the number of individuals to be tested to detect linkage with a QTL of giYen effect (81).
Assuming that the marker is the QTL, the number of individuals to test in order to detect an effect of given amplitude, ~, can be estimated from:

21005~
W092/13102 PCT/US92/003~0 --19-- ., 4 (to+tl) 2. S2 n > 2 3 where n gives the required sample size, 52 is an estimate of the residual variance, to is the t value associated with Type I error, and t1 is the t value ssociated with Type II error; tl e~uals tabulated t for probability 2(1-P) where P is the required probability of detecting ~ if such a difference exists (82).
For dairy production for instance, and if perform ing the linkage analysis using the "daughter yield deviations" (DYD; a2D~ 600 lb) from paternal half.-sibs ("granddaughter design" t83), one would have to study 1,500, 37~ and ~68 individuals, respectively, to cletect QTL with differences of 200 lb, 400 lb and ~00 lb between alternate alleles. ~ssuming phenotypic vaxiance of ~2500 lb)2, such effects correspond to 0.08, 0.16 and 0.24 standard deviations, respectively. These estimates assume a Type I error o~ 5%, a Type II error of 10%, and absence of recombination between marker and QTL.
If the tested marker and the QTL recombine at a rate e, the number of individuals to test increases by a factor l/ ( l-2e) 2 for single marker analysis, by a factor #(l~ -2e) 2 in the case of interval mapping, 7 corresponding to the recombination rate between the flanking markers (8l).
In view of the costs and time involved in geno-typing, it is important to minimize the re~uired sample size. This can be achieved in various ways:

a. Identi~ication.of the Individuals Most Likely to be Heterozyqous. hence In~ormative, for the Studied OTLs Onç way to achieve this is to cross highly diver-gent strains for the trait of interest. In plant breeding, where the use of exotic germplasm is common practice, this is perfectly applicable~ The .

WO92/13102 210 0 5 8 3 PCT/US92/0034~

identification of markers for interesting QTLs from the exotic strains can then be used for their marker assisted introgression in the commercial varieties.
Tn animal breeding, however, introgression programs are very uncommon. With "Velogenetics" (described further below), however, the use of exotic germplasm in introgression programs may become more attractive for animal breeders as well.
An alternative approach is to identify the indi-viduals whose offspring are showing a higher variance for the trait of interest.

b. Selective Genotypin~ of_the Extreme Pro~enY
As pointed out by Lander and Botstein (75), the individuals whose genotype can be most clearly inferred from their phenotype are the ones providing most of the linkage information when studying complex traits. For quantitative traits, these are the individuals whose phenotypic value deviates most from the mean: the tails of the distribution. Sample sizes could be reduced by 60% and even 80% by focusing on individuals deviating one and two standard de~iations, respectively, from the mean. Paradoxically, selective genotyping may be limited by the size of the studied population. Indeed, a larger sample will be required in order to find enough individ-uals one or two standard deviations from the mean.

c. Decreasing Environmental Va~iance via Proaeny Testina Weller et al. (83), have tested the effect of progeny testing to reduce the environmental variance by comparing the power of "daughter" and "granddaughter"
designs for the dete~tion of QTLs in dairy cattle. In the "daughter" design, marker genotype and guantitative trait values are assessed on daughters of heterozygous sires, while in the "granddaughter" design, marker genotypes are determined on sons of heterozygous sires, ~092/13102 2 1 ~ O ~ ~ 3 PCTJUS92/00~0 their breeding values being determined by progeny testin~ from the quantitative trait value measured on their daughters. They demonstrate that for equal power the "granddaughter" design requires half as many marker assays as the "daughter" design.

d. Reducing genetic noise by searching for ~everal unlinked QTL simultaneously, or "simultaneous" search t8l).

e. Usina DNA Pools Instead of genotyping all individuals separately, one can analy~e DNA pools from individuals sorted by phenotype. Significant differences of allelic fre-guencies between pools point towards possible genetic linkage between the corresponding marker locus and a gene or genes affecting the trait of interest. This approach can be used both for "within family" studies and for studies at the population level. The latter approach, however, requires linkage disequilibrium between QTL and mar~er locus. This method was first described by Arnheim et al. (84) to study the role o~
HL~ class II loci in insulin-dependent diabetes mellitus. It was recently adopted by Plotsky et al.
(85) to study association between DNA fingerprint bands and abdominal fat deposition in broilers.

f. Ex~loitin~ "Taqqed QTLs"
The direct effect of selection for a production trait will be to increase the frequency of the favorable alleles at the segregating QTLs. However, this selection pressure may indirectly affect loci in linkage disequi-librium by so-called "hitch-hiking".
This is probably what happened to the genetic defect causing progressive degenerative myeloencephalo-pathy, or Weaver in Brown Swiss, shown to be linked to a major gene for milk production. Because of the WO92/13102 2 1 0 0 5 ~ 3 PCT/US92/00340 deleterious effect of the Weaver causing gene, it is the heterozygous "carrier" genotype which is selectively most advantageous, generating a "balanced polymorphism", the Weaver causing allele being maintained in the population at a relatively high frequency. This can be exploited, however, to map the corresponding QTL by going through the relatively easy exercise (compared to QTL mapping~ of finding a marker linked to this single gene disorder. We have recently identified a marker linked to Weaver and presumably to the associated QTL.
Besides Weaver, QTLs for a variety of polygenic traits have been identified, both in plants and animals.
Using complete DSP maps in tomato, Paterson et al. (78) identified at least six genes controlling fruit mass, four controlling soluble solids, and five controlling fruit pH, accounting for 58%, 44% and 48%, respectively, of the phenotypic variance. Martin et al. (79), using a similar approach, identified at least three tomato genes controlling water use efficiency. In cattle, Geldermann et al. (86) found significant effects on milk yield (+ 200 kgæ) and fat content (~ 1%), especially for the B~lactoglobulin locus. Morè recently, Cowan et al.
(87) demonstrated significant effects on milk production traits using a prolactin DNA Sequence Polymorphism as marker.

B. OTL Mappina Within Po~ulations One can expect to find an effect of marker alleles linked to QTLs also outside of a family context, i~e., at the population level, if the two loci are in linkage disequilibrium. As reported by Hanset (88), and assuming a diallelic marker (alleles Ml and M2 with respective frequencies pl and p2) linked to a diallelic QTL, the phenotypic difference between the respective homozygotes 210 0 ~ 8 3 WO92/13102 PCT/US92/0034n at the marker loci is: j D t = 2a .
pl.p2 i with D measuring the linkage disequilibrium and 2a cor-responding to the phenotypic difference betwean the two homozygotas for the QTL.
Markers for which a priori evidence for linkage disequilibrium is highest are the so-called "candidate genes": genes expected from their physiological role to be likely candidates for the QTL itself. DSPs at those loci, even selectively neutral by themself, can be expected to exhibit linkage disequilibrium with the hypothetical functional mutations because of their very tight linkage. As an example, the B allele of the K-casein gene has been shown in several studies to increase protein yield in milk by about 3%, and possibly to improve cheese yield independent of the effect on protein yield (see, for instance, 89, 90).

IV. USE OF DNA MARKERS IN B~EEDIN~ PROGRAMS

In classical selection programs, breeding values are estimated from individuals' own performances and performances of relatives (136). The exp~cted genetic progress is a function of the accuracy of selection, i.e. the correlation between estimated and true breeding values. All direct information on QTL can be used to increase the accuracy of selection and, hence, genetic response. Early on, Soller and Beckmann (9l) proposed to exploit marker information for the preselection of young dairy sires before progeny-test. In cattle, Marker Assisted Selection is already used for the sexing of preimplantation embryos using Y-specific probes (see, for instance, 92), and for genotyping at the X-casein (see, for instance, 93) and prolactin loci (87). In WO9~/1310' 2 1 ~ 0 5 ~ 3 PCT/~'S92/00~40 pigs, Marker Assisted Selection is used to reduce the frequency of the major gene causing Porcine Stress Syndrome ~PS~). Susceptibility to PSS correiates with Halothane sensitivity or Malignant Hyperthermia. This condition has been mapped to a linkage group on pig chromosome ~, encompassing the following markers: S(A-O)-GPI-Hal-H-AlBG-PGD ~reviewed by 94). These markers are used ~or the ~arkèr Assisted Selection against the PSS condition. Recently, the ryanodine receptor gene has been identified as a good candidate for the Malig-nant Hyperthermia or Hal gene (95).
As shown by Smith and Simpson (96), the gain to be made with M rker Assisted Selection increases with the proportion of QTL identified and is highest for low heritability traits. Unfortunately, the QTL determining the latter traits are also the hardest ones to identify.
It should be noted that the increase in accuracy is subordinate to the accurate estimation of the QTL
effects. This may require larger samples than the ones needed for the detection of linkage. Once a QTL mapped by within-family linkage studies, it may be more effec-tive to identify supplementary flanking markers and to accurately determine the effect of the generated haplo-types at the population level. Selection can then focus on the best haplotype instead of spending initial selec-tion efforts on intermediate ones.
The use of genetic markers in selection programs may as well reveal dominance deviation (particularly overdominance) and interaction deviation at defined QTL, variance components poorly dealt with in classical breeding theory. Specific programs may be required to fully exploit these QTL. In the case of overdominance for instance, two lines each homozygous for the differ-ent alleles at each QTL could be developed and crossed to produce multiple heterozygotes.
There is widespread interest in resolving quantitative traits into their Mendelian components by WO92/13102 2 l e ~ 5~ PCT/VS92/00340 mapping the underlying QTL. The implementation of marker assisted selection into breeding schemes, however, has not always been received with enthusiasm. Part of the skepticism expr~sses the doubt that the genetic gains obtainable by marker assisted selection will justify expensive and tedious large scale gPnotyping. Although the costs of genotyping will drop substantially in the near future, due to the rapid pace at which automation and robotics are being applied to DNA technology, this objection remains very pertinent.
Another major limitation of marker assisted selection under its present form, is its limitation to the exploitation of genetic variation preexisting within the commercial breed of interest, and only that present in a "high merit" genetic background. Favorable mutation~ appearing within a mediocre background, or present in "axotic" germplasm, would be difficult to exploit, even with markers.
We have therefore proposed a scheme, designed as ~'Velogenetics", combining marker assisted introgression and germ-line manipulations to reduce the generation interval, which might drastically increase the power of marker assisted selection (141).

IV. INDIVIDUAL IDENTIFICATION AND PATERNITY DIAGNOSIS:

Methods to estimate the breeding value of an animal use information from relatives. As a matter of fact, keeping track of familial relationships has always been one of the major concerns of animal breeders, and parentage control is now a widely used procedure for several domestic species. Parentage control relies on the use of polymorphic systems within the studied population. The alleles that characterize an individual originate from the mother or the father. If one of the parents is known (usually the mother), the alleles necessarily transmitted by the other parent can be WO92/13102 2 1 0 0 ~ ~ 3 PCT/~IS~2/00340 deduced easily. Paternity testing consists of scoring the existence or la~k of those obli~ate paternal alleles in the genotype of the put~tive parent. Lack of one or more of these alleles points towards incorrectly assigned paternity. If, on the contrary, all obligate paternal alleles are present in the tested parent, there is no evidence for incorrectly assigned paternity.
Nevertheless, one always has to consider ~he possibility of fortuitou~ coincidence. The higher the variation of the genetic markexs used, the higher the probability to detect incorrectly assigned paternity, thus the higher the "exclusion power".
Until now, the systems most often used for paternity testing were blood group systems, biochemical polymorphisms, or the major histocompatibility system.
The availability of DSP, however, opens new perspectives for paternity dia~nosis. Hypervariable minisatellites in particular, characterized by their remarkably high degree of polymorphism, have proven especially useful in this respect. Multilocus DNA fingerprints, based on the simultaneous detection of related minisatellite loci, have been shown to be extremely powerful for paternity diagnosis, both in human (l9~ and animals (108, lO9)~
Exclusion powers as high as 99.999996~ have been 2S obtained with as few as 2 probes in the human (l9).
With such high exclusion powers, absence of exclusion can be considered proof for true biological parentage~
Another corollary is that very high exclusion powers can be obtained even when a single parent is available and tested for parenthood. Multilocus DNA fingerprints, however, tend to be replaced by the combined use of a limited number of locus-specific VNTR markers (20), gi~ing equally powerful, but more reproducible, sensitive and easily interpretable patterns. With the 3~ advent of locus-specific VNTRs and PCR-amplifiable microsatellites in animal species (44), the same will probably hold in this field too.

21~05~3 Along the same lines, DNA markers can be used as well for individual identification. Using expansion-contraction type polymorphisms, individual specific "DNA
bar codes" can literally be generated (l9, ll0).

SUMMARY OF THE INV~NTION

Disclosed herein is a set of locus-spscific genetic markers for domestic cattle and related bovids, that constitute a primary bovine DNA marker map. Among other applications, these markers and the map are useful for:

- individual identification, - parentage testing, - the genetic mapping of economic trait loci, or genes involved in the determinism of economicalli important traits, whether single gene traits or - complex multifactorial traits, - marker assisted selection, - velogenetics, or the synergistic use of marker assisted introgression and germ-line manipulations to reduce the generation interval.

The usefulness of this set of markers for the genetic mapping of economic trait loci is illustrated by the identification of a genetic marker for bovine progressive degenerative myelo-encephalopathy or "Weaver" in the 8rown Swiss braed.

BRIEF DFSCRIPTION OF_~HE FIG~RES

Figure l: shows a typical VNTR pattern obtained with probe GMBT-005, usin~ ~aeIII.
Figure 2: Example of a microsatellite pattern (TGLA9).
Figure 3: Schematic representation of "Velogenetics".

WO92/1310~ 210 0 5 ~ 3 pcT/~lss2/oo34n DETAILED DESCRIPTION OF~THE INVENTION

I. CONSTRUCTION OF A PRIMARY BOVINE DNA MARKER MAP:

Our laboratory has focused in the last two years in the development of a primary DN~ marker map for cattle. We have now developed more than 300 highly polymorphic DNA
markers of either of three types:

1. Variable Numbe~_of_ Tandem Repeat Markers rVNTR~
~ypervariable minisa~ellites are known t:o show significant cross-hybridization between species (31, 44, 19 110). We havP exploited this to isolate bovine VNTRs using heterologous minisatellite probes. Screening purpose-built libraries with minisatellite probes, we have isolated 36 bo~ine VNTRs, characterized by a mean heterozygosity of 59.3% within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Nonte-Carlo simulation, showing that the top 5 to 10 probes could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids and in situ hybridization demonstrate that these bovine VNTRs are organi2ed as clusters, scattered throughout the bovine genome, without evidence for proterminal confinement as in the human (35). Moreover, Southern blot analysis and situ hybridization demonstrate conservation of sequence and map location respectively of minisatellites within Bovidae. A typical VNTR pattern obtained with one of our probes is shown in Figure 1. Detailed description of our VNTR systems is reported in "Example 1".

~1~0~ 3 WO92/13102 PCT/~IS92/00340 2. Multisite haplotypes ', We used 110 random cosmids to probe Southern blots of 9 unrelated rattle DNAs digested with 12 restriction enzymes. Although only one third of the expected fragments could be detected, 85% of the cosmids revealed at least one polymorphism. Th~ mean heterozygosity of the generated multisite haplotypQs (98~ was estimated at 51.9% . A surprisingly high proportion of polymorphisms (~Z5%) was attributed to insertion-deletion events, compensa~ing for the lower level of nucleotide diversity, ~, observed in cattle (~ z 0.0007) as compared to the human. The mutation rate at cyt:osines in the CpG dinucleotide was estimated approximately 10 times higher compared to other nucleotides. The generated markers should cover approximately 40% of the bovine genome when used in linkage studies. A detailed descrip~ion of our multisite haplotypes is reported in "Example 2".

3. Microsatellites Recently, microsatellites were proven to be an abundant source of highly polymorphic markers in the human (32-34). As their name implies, microsatellites are minute VNTR markers (18-20), characterized by tandem repetitions of very short repeats, one to four base pairs in length. Microsatellites exhibit levels of polymorphism comparable to VNTRs, but are much more abundant and apparently evenly spread throughout the genome. We have estimated the frequency of (CA)-dinucleotide repeats in the bovine genome at ~ 150,000.
Because of their small size, their detection is greatly facilitated by PCR. Although this imposes the preliminary determination of flanking DNA sequences to design the appropriate primers, the ~ubsequent PCR
reaction used for their analysis offers several advantages over Southern blotting, being fast, requiring less DNA and being easier to automate.

WO92~13102 Z ~ 3 PCT/US92/00340 `

As part of our effort to build a primary DNA marker map for cattle, we have isolated more than 250 bovine s microsatellites, amplified most of ~hem in vitro and shown that the majority of them are indeed polymorphic in cattle. Several of these have been tentatively assigned ~o specific bovine chromosomes using a somatic cell hybrid panel. Moreover, we have shown that approxima~ely 50% of the bovine microsatellites can be successfully used in other Bovidae as well, which will greatly facilitate the construction of marXer maps in these species.
Magnetic solid phase DNA sequencing procedures (137) are used for the massive generation of sequence information and multiplex approaches for genotype collection, based on the simultaneous detection of molecules labelled with different fluorescent dyes using a laser-excited confocal fluorescence gel scanner ~139).
A typical microsatellite pattern is shown in Figure 2. A detailed escription of our microsateIlites is reported in "Example 3".

The relative location of the markers was determined by linkage analysis in pedigrees generated by multiple ovulation and embryo transfer. To assign linkage groups to specific chromosomes, highly polymorphic "anchor markers" were mapped using somatic cell hybrids (Jim Womack, Texas A&~), and by in situ hybridization ~Rudy Fries, ETH - Zurich).
Linkage analysis involving l50 of these markers, generated a primary DNA marker map with 24 linkage groups counting two or more markers (lS assigned to specific chromosomes or synteny groups), and 68 singleton markers. A detailed description of our pri-mary bovine DNA marker map is reported in "Example 4".

-- 21~0583 ~092/13102 PCT/US92/00340 -3l-II. MICROSATELLITE MAPPING OF A MAJOR GENE FOR MILK
PRODUCTION, LINgED TO BOVINE PROGRESSIVE DEGENERATIVE
MYELOENCEPHALOPATHY OR WEAVER.

Identifying polygenes, requires the analysis of pedigrees of considerable size, despite the development of procedures such as int~rval mapping, simultaneous search, selective genotyping, etc. In this work we have explored an alternative approach to map a polygene, exploiting the association observed in cattle between the single gene disorder "Weaver", and increased milk production. Weaver or bovine progressive degenerative myeloencephalopathy is a rece~sive disorder characterized by the appearance between 5 and 8 months of age of bilateral hind leg weakne~s, ataxia with deficisnt proprioceptive re.flexes, without skeletal or muscular defects. Estimates of gene fre~uency in the American Brown Swiss breed point towards the maintenance of the Weaver gene at relatively high frequency (>5%), despite the implementation of programs for detection and elimination of carrier bulls. Moraover, Hoeschele and Meinert (140) showed that Weaver carrier animals have an advantage of 690.8 kgs milk (~ 0.25 phenotypic a) above the mean. Both observations could be accounted for by the presence of a gene with major effect on milk yield in linkage disequilibrium with the "weaver" gene.
Brown Swiss animals showing symptoms of Weaver were identified with the help of the American Brown Swiss ~ssociation. Blood samples were collected from the affected animals, their parents, and full-siblings when available. Diagnosis of Weaver was confirmed in most cases by anatomopathological examînation of spinal cord and cerebellum at the Department of Pathology of the College of Veterinary Medicine, Kansas State University.
Shrunken Purkinje cells in the cerebellum, spheroids and degenerated myelin sheets in the spinal cord were considered pathognomonic. Altogether, 78 animals were 2100~
WO92/1310~ PCT/US92/00340 identified generating a single, large pedigree. All animals were genotyped for more than 70 genetic markers:
40 Variable Number of Tandem Repeat markers and more than 30 Microsatellites. Linkage analysis was performed using the "LINKAGE" programs (60). The microsatellite marker T~LA116 was giving a highly signifi~ant lodscore o f 6 . 5 for a recombination rate of 7 . 5% . Although a priori probability for pair-wise linkaga is unknown in cattle, a lodscore of 3 is generally considered to be the threshold for statistical significance as in the human . This value ( 5 . 8 ) was obtained assuming complete penetrance~ Actual penetrance for the Weaver condition is unknown. However, and because our pedigree was constructed by sampling clearly affected animals, the assumption of complete penetrance is v ry reasonable in this situation.
The marker TGLA116 is characterized by three alleles segregating in our Weaver pedigree. 72% of the affected individuals were of the 3/3 genotype, 16% of the 2/3 genotype, and 12% of the l/3 genotype. Hence, and at least in our family material, the "Weaver" allele was clearly a~sociated with allele 3 at the marker locus. Whether ~imilar disequilibrium will be observed at the population level remains to be determined. The reported lodscore values were obtained using allelic frequencies estimated on a sampl of 135 sires from the American Brown Swiss breed.
Because of the biased sampling procedure used to generate the pedigree markers showing distorted segrèga-tion could generate erroneous evidence for linkage with the disease. A "control" pedigree, con~isting of more than lO0 Weaver-free Holstein individuals, was therefore typed for TGLA116 as well. The microsatellite marker was characterized in this pedigree by the same three alleles, with respective frequencies of 18%, 57% and 25~
for alleles l, 2 and 3, showing a perfect Mendelian segregation. Therefore, it is concluded that marker WO92/13102 2 1 0 ~ S ~ 3 PCT/~1~92/00340 TGLA116 is genetically linked to Weaver. From the generated lodscore curves, the genetic distance between the two loci is estimated at 3 + 10 centiMorgan. The limlts of this 95% confidence interval correspond to recombination rates with lodscores one unit below the obtained maximum lodscore. Because of the tight linkage between TGLA116 and Weaver, this marker should be linked to the associated QTL as well. The distance between TGLA116/Weaver and QTL is, however, unknown at this point. The effect using Weaver as marker, however, was of such magnitude that the genetic distance separating these loci is unlikely to be great. we are in the process of determining the relative location of these three loci.
In consequence, the TGLA116 marker will allow us to perform marker assisted selection against the Weaver condition. Indeed, it is now possible for offspring from individuals heterozygous for both the Weaver condition and TGLAll~, to estimate the genotypic likelihoods at the Weaver locus basad on their TGLA116 genotype and that of their parents.
In addition, we are now in a position to test the effect of the corresponding chromosomal segment on milk production.

III. VELOGENETICS

Few ~uestion the fundamental interest of resolving quantitative traits into their Mendelian components by mapping the underlying QTL. The implementation of Marker Assisted Selection into breeding schemes, however, has not always been received with a lot of enthusiasm. Part of this skepticism reflects the disbelieve that DNA
Marker Maps will become available for our domestic species within a reasonable time-span, or that QTL can be identified by linkage strategies. In our view, these arguments only reflect the lack of information of their 21~ 1~ 583 -`
WO9~/1310~ PCT/~IS92/00340 protractors. On the other hand, part of the skepticism expresses the doubt that the genetic gains obtainable by Marker Assisted Selection will justify expensive and tedious large scale genotyping. Although the costs of genotyping will drop substantially in the near future, due to the ama~ing pace at which automation and robotics are applied to DNA technology, this objection remains quite pertinent.
Another major limitation of Marker Assisted Selec-tion under its present form, is its limitation to the exploitation of genetic variation preexisting with.in the commercial breed of interest, and only if present in a "high merit" genetic background. Favorable mutations appearing within a mediocre background, or pres~ent in "exotic" germplasm, would be difficult to exploit, even with markers.
We propose a scheme, combining Marker Assisted Introgression and germ-line manipulations, to reduce the generation interval -- which might drastically increase the power of Marker Assisted Selection: "Velogenetics"

A. Marker Assisted Introqression The basic principle underlying Marker Assisted Introgression are well-known. A gene responsible for a favorable attribute can be introgressed from a "donor"
strain into a "recipient" strain by repeated backcross-inq. During the introgression process, the retention of the favorable gene is monitored in the backcross pro-ducts, with linked, flanking DNA markers. This latter aspect is particularly important for traits involving multiple genes and/or characterized by sex- or age-limited expression. Classical genetic theory tells us that, with the exception of the "marked" segment whose retention is desired, the genomic contribution of the donor line is diluted by half after each backcross.
Hence, and after four backcrosses, the recipient genome is reconstituted to + 90% of the original. At the - ~100~3 WO9~/1310~ PCT/US92/0034() marked locus, however, the ba~kcross retains one copy of the desired "donor" variant. If required, one intercross will then generate 25% of offspring homozygous for the favorable donor variant. The net result is a "graft" of an advantageous gene within a recipient background. The procedures entirely respects organization and chromo-somal localization of the grafted gene, avoiding aberrant expression patterns, which are too often characterizing tra~sgenes.
Noteworthy, the gene to be transferred does not need to be cloned ~er se. Only its genetic map location is required, as defined by the availability of linked markers, ideally flanking the gene of interest on each side. Hence, this procedure is perfectly applicable for the introgression of QTL identified through the pre-viously described mapping strategies.
~arker Assisted Introgression can easily be applied to several genes simultaneously. This feature will be of particular interest for complex traits involving several genes. Introgressing more than one gene from a donor to a recipient line, however, increases the sel~ction intensity at each backcross: with l marker, l/2 of the offspring have the favorable genotype, with 2, l/4 and with n markers, (l/ 2 ) n .
Selecting for the retention of defined "donor"
genes will hamper the recovery of the recipient back-ground aenotype in adjacent chromosomal regions. This can be compensated for by increasing the number of backcrosses, or better by monitoring the fate of addi-tional adjacent markers to identify the backcross products resulting from recombinations as close to the "grafted" gene as possible.

B. Shortenina the Generation Interval of Domestic SDecies bv "Veloaenesis"
Introgression by repeated backcrossing, assisted or not by genetic markers, is common practice in a ~ariety 210~5~3 -~WO 9?/1310'' PCr/ussZ/0034n of organisms, but is essentially unfeasible in domestic animals such as cattle, because of their prohibitively long generation time~ The generation interval of such species could, however, be reduced b~sed on the "in vitro" maturation and fert'lization of foetal oocytes, hereinafter referred to as "Velogenesisl'.
An overview of female gametogenesis (loo,lOl), indicates that the feasibility of such a scheme may not be that far-fetched. Briefly, oogenesis begins with the formation of primordial germ cells in the region of the allantois. These precursor cells migrate to the developing gonads where after a period of .mitotic proliferation, they enter meiosis. Meiosis is arrested at the diplotene stage of prophase I by the poorly understood l'meiotic division I arrest system", after which the primary oocyte enters a resting phase. During the life time of the animal, small numbers of resting primary oocytes are successively recruited into a pool of growing oocytes, within the environment of a gonadotropin-dependent developing follicle. These activated oocytes growth in size, acquire the competence to resume meiosis if appropriately stimulated, and accumulate the required material to sustain the early stages of the subsequent embryonal development.
Resumption of meiosis and oocyte maturation is triggered by a hypothetical maturation-inducing signal produced by granulosa cells in response to gonadotropins. At least in rodents, oocyte maturation seems to be mediated by a drop in cyclic AMP in the oocyte and subsequent inactivation o~ a type A protein kinase. Evidence for the role of this pathway in oocyte maturation is, however, much more controversial in ruminants. Note that in the granulosa cells, gonadotropins act, among other pathways, throu~h the activation of adenylate cyclase with subsequent increase in cAMP concentrations (102). In the oocyte, a cascade of still to be deter-mined events then probably leads to the phosphorylation WO92~13102 21~ 3 PCT/US92/00340 and activation of a phosphatase, probably homologous to the S. Pombe cdc25 gene (103), which will itself dephos-phorylate and activate the M-phase promoting factor (MPF), now known to be a complex of a p34cdC2protein kinase subunit with a 8 type cyclin (see 104 for a review3. The maturing oocyte completes the first meiotic division and enters the second (becoming a secondary oocyte) which will be arrested as well at metaphase II until fertilization. This "meiotic division II arrest system" is thought to reflect the stabilization of MPF mediated by the kinase activity of pp39~5 on either a cyclin protease or on cyclin itself.
Fertilization relieves this block, by increasing the intracellular Ca2+ concentration, triggering calcium-dependent protease activity (reviewed in 104)o In cattle, primordial germ cells reach the genital ridge at about 40 days of gestation. After a period of mitotic proliferation, they differentiate into oogonia starting around 60 days of gestation. Mitotic prolifera-tion of the germ line ceases around day 170 of gestation fixing the maximum number of oocytes the female will ever have. Meiosis starts at about 80 days, and the first primordial follicles are discernable at 90 days of gestation. Remarkably, activation of resting primordial follicles starts already in utero, around day 140, and secondary and tertiary follicles can be seen at 210 and 230 days, respectively. Tt is estimated that 2 to 4 resting primordial follicles are recruited daily into the pool of activated, developing follicles. These activated foetal oocytes, however, are irrevocably committed to follicular atresia. Indeed, spontaneous oocyte maturation and ovulation do not begin until puberty. Submitted to appropriate hormonal stimulus, however, prepubertal oocytes can resume meiosis, can be fertilized and can produce viable offspring. Indeed, offspring have been obtained from gonadotropin-stimulated calf oocytes, transferred to postpubertal 2100~3 W092/131~2 PGT/US92/00340 recipient animals (reviewed in 105). The purpose of velogenesis would be to attempt to obtain similar results with foetal oocytes at the earliest stage possible, as early as 90 to 180 days of gestation.
Very encouraging is the development in mice of culture systems supporting the growth of primary folliclesf yielding mature oocytes capable of fertil-ization in vitro and develop~ent to term (106, 107). It is reasonable to anticipate that similar conditions, supporting development of bovine oocytes, will become available in a species were primary oocytes from relatively small antral follicles can already be successfully matured and fertilized ln vitro.
On way to achieve velogenesis would be to attempt to rescue oocyte nuclei from primordial follic:les by their transfer into enucleated, ma~urable oocytes.
So far we have only discussed velogenesis throu~h the reduction o~ the female generation interval. "Male"
velogenesis could similarly be accomplished by the early stimulation of spermatogenesis.
The impact on breeding programs of "velogenesis" or the reduction of generation time by n vitro maturation and fertilization of fetal oocytes has been discussed by Betteridge et al. (101). In dairy breeding, for instance, annual responses in milk yield could be doubled compared to conventional progeny testing. With the added power of Marker Assisted Introgression, the approach becomes much more powerful. "Velogenetics", or the synergistic use of Marker Assisted Introgression and "velogenesis", can be viewed as a procedure for the rapid and efficient intraspecies transfer of desirable genes between genetic backgrounds. By analogy with the term "transgene", the manipulated genes are referred to as "velogenes".
In particular, desirable traits identified cutside commercial breeding stock, could be quickly introgressed into high merit genetic backgrounds. Examples would 2 1 ~ } 3 WO92/13102 PCT~'S92/0034fl include disease resistance, genes affecting milk and meat composition, ~olled, coat color genes, etc. More- ii over, the possibility to exploit "exotic" genetic variation identified outside th~ breed of interest is particularly attractive because it greatly facilitates the mapping of the genes of interest.
A schematic representation o~ "Velogenetics" is shown in Figure 3.

The present invention is further detailed in the following Examples, which are offered by way of illus-tration and are not intended to limit the invention in any manner. Standard techniques well known in the art or the techniques specifically described below were utilized.

C~RACTl~RIZATION OF
A 8ET OF ~RI~L~ SR OF TANl)~ ~PEAq! ~8 CON8ERVE:D 2N BO~IDAE.

INTRODnCTION

Human minisatellite sequences, exhibiting very high levels of genetic polymorphism due to variation in the number of tandem repetitions, have proven an invaluable source of genetic markers commonly termed "VNTRs" (18-20). VNTRs have been instrumental in the genetic mapping of several disease-causing genes, as tools for individual identification and paternity diagnosis and to address a variety of biological issues, including imprinting, loss of heterozygosity in malignancies, etc.
In animal genetics, highly polymorphic markers such as VNTRs could similarly be used for individual identification and paternity diagnosis - relying today on less informative biochemical polymorphi5ms and blood 2 1 0 0 ~ 8 3 ~
WO92/13102 PCT/~'S92/00340 group systems -, and for the mapping of so-called economic trait loci (ETL) or genes involved in the determination o~ production traits. Classically, artificial selection has relied on the biometrical evaluation of breedin~ values from individual performance records and from performance~ of relatives (136). One of the powers of the biometrical approach is that it obviates the need for any detailed molecular knowledge of the und~rlying genes or ETL. However, it is believed that the genetic mapping of ETL could be used to increase genetic response by affecting accuracy and speed of selection, through a procedure called marker assisted selection (MAS) (91, 96). Moreover, defined alleles could be moved efficiently between genetic backgrounds by velogenetics or the combined use of marker assisted introgrecsion and germline manipulations aimed to reduce the generation interval (141, 142).
WP report the cloning and characterization of 36 bovine variable number of tandem repeat (VNTR) markers, characterized by a high degree of polymorphism ~ithin commercial herds and shown to be conserved within Bovidae.

MATBRIA~8 AND METXOD8 1. Clo~ing of bovi~ VNTRs and ~etection of polymorphi~:
! 500~g genomic DNA from 20 unrelated cows was digested to completion with MboI or HaeIII. After two rounds of size fractionation by agarose gel electrophoresis, electroelution and addition of EcoRI
linkers (only for HaeIII restricted DNA), fractions from 3 to 4 Kb (kilobases), from 4 to 6 Kb and above 6 Kb were ligated into the BAP-déphosphorylated BamHI and EcoRI sites, respectively of pUC13. Approximately 80,000 independent clones were obtained by WO92/13102 210 Q 5 8 ~ PCT/~'S92/00340 -4l-transformation of DH5~ cells, and were screened successively with the following minisatellite sequences:
- the 282 base pair HaeIII-ClaI fragment containing the minisatallite in the protein III gene of wild-type Ml3, ~r pUCJ, pSP64.2.5EI ('Per')~ p~3'HVR64, pINS310, EFDl34.7 and pS3 (20, 21, llO, 123, 143). Hybridization and washings were done in the conditions used to generate multilocus DNA fingerpxints with the respective probes (110, 143). Tc check for polymorphism, plasmid DNA
isolated from positive colonias was used to probe MboI, HaeIII and TaqI Southern blots of 18 randomly selected Americ n Holsteins. Hybridizations were done at 65 C in 7% SDS, 10% PEG, 50mM NaHPO4 with addition of '50~g/ml bovine genomic DNA. Final washes were at 65 C in O.lxSSC, 0.1% SDS. When using bovine probes on ovine Southern blots, hybridization and washing temperatures were reduced by lO C.

2. ~ti~tion of N~tchi~g Probabilities ~n~
~clu~ion Po~er~:
Allelic frequencies were estimated from the sample of 18 randomly selected American Hol~teins. Matching probabilities and exclusion powers (113) were then estimated by Monte-Carlo simulation (lO,000 simulations in each case), assuming Hardy-Weinberg e~uilibrium and using "Pat-Power", a program designed by one of us. The following parameters were estimated: MPR: matching probability for two randomly selected individuals; MPS:
matching probability for full-sibs; EPR: probability to exclude an alleged father unrelated to the real one (mothers phenotype known); EPS: probability to exclude an alleged father full-sib to the real one (mothers phenotype known): EPl: probability to exclude a wrongly assigned parent without phenotypic information available from the other one.

210~3 --WO92~13102 PCT/US~2!00340 Patpower calculates Matching Probabilities and Exclusion Powers characterizing given autosomal poly- 3;
morphic systems, by Monte-Carlo simulation.
Matching Probabilities relate to individual identi-fication and express the lîkelihood that two individuals would have the same pattern with a ~iven probe. Patpower calculates two types of Matching Probabilities: MPR, the Matching Probability for two unrelated individuals, and MPS, the Matching Probability for two full-sibs.
Exclusion Powers relate to paternity diagnosis and express the likelihood that a wrongly assigned paternity or maternity will be detected with a given probe.
Patpower determines three types of Exclusion Power: EPR, where one parent is known with certainty, the proband is unrelated to the other real parent; EPS, where one parent is known with certainty, the proband is full-sib of the other real parent; and EPl, whre only the proband is available.
~he user needs to input the number of alleles char-acterizing the polymorphic system in the population of interest, their respective frequencies, and their dominance-recessivity relationships. For the AB0 blood group system in humans, for instance, A and B are codominant and both dominate 0. Each allele is given a binary code following the rules of the "LINKAGE" program (60).
Patpower then stochastically generates a pair of parents with an offspring, a full-sib of the real father and an unrelated individual. "Phenotypes" are obtained from the genotype using the boolean "or" operator and are used to determine matching between unrelated indivi-duals (MPR) and between full-sibs (MPS), as well as the exclusion of the unrelated individual considered as a proband, with (EPR) and without information ~EPl) from one of the real parents, and exclusion of the uncle considering information from the real mother (EPS).
This simulation is repeated as many times as determined WO9~/1310~ 210 ~ 5 ~ ~ PCTIUS92l00340 by the user, allowing for the estimation of the raspec-tive livelihoods.
~1 3. ~egrog~tio~ ~na li~ag~ analysi~
All members of an American Holstein pedigree with 91 offspring obtained by multiple ovulatiun and embryo transfer (MOET) from 20 parants, were genotyped for all identi~ied markers. Each parent has a mean o~ g.1 o~fspring with a mean of 1.9 partners. Segregation and linkage analysis were don with slightly modified ~ersions of the "LINKAGE" programs as previously described (31).

4. ~ys~teny mappi~g:
The hybrid somatic cells were prepared by fusion as previously described (97). Southern blot hybridi~ation and concordancy analysis were done according to Threadgill et al. (114).

5. I~ situ hybri~is~t~on:
Chromosomes were prepared as described by Fries et al. (115) and chromosome identification was based on QFQ-banding and according to the international standard (116). Probe preparation and in situ hybridization were as previously described (144).

RB8~T~
. A ~et o~ bovi~e VNT~ marker~:
Using the strategy described above, we have isolated a total of 36 bovine VNTRs, listed in Table l.
Polymorphic patterns were attributed to minisatellite sequences when characterized by more than two alleles distinguishable with more than one restriction enzyme.
Seven additional, non VNTR-type polymorphisms were detected during this experiment and are reported as well~

21~0583 -~ `

VN~R Clones Name Lo~usl Polymorph2 Enz. 3 Het. Ovin GMBT-002 DYl6Slb VNTR aeIII 52 P
GMBT-003 ~NTR ~III 56 ÇMBT-OOS D24Sib VNTR HaeIII 85 N
GMBT-006 Dl4Sib VNTR HaeIII 73 N
GMBT-007 DUlOSib VNTR ~3~III 96 P
GNBT-O08 VNTR ~3~I P
GMBT-009 DU22Sib VNTR ~aeIII 58 GMBT-Oll D26Sib ~NTR HaeIII 85 P
GMBT-012 VNTR MboI 22 GMBT-013 VNTR HaeIII 4 GMBT-OlS D21S3b VNTR HaeIII 61 P
GMBT-0~6 D21Sl2b VNTR ~3~III 78 P
GMBT-0l7 D8Sib VNTR HaeIII l5 M
GMBT-O~9 DlOSib VNTR MboI 7 G~BT-020 VNTR MboI 65 GMBT-021 D21S2b VNTR HaeIII 65 GMBT-022 Dl8Sib VNTR MboI 40 GMBT-025 VNTR Ha~III 25 GMBT-026 VNTR HaeIII 26 GMBT-027 VNTR MboI 40 GMBT-028 D2Sib VNTR HaeIII 81 GMBT-031 VNTR ~3~III 58 GMBT-033 VNTR ~III 70 GMBT-034 VNTR HaeIII 20 ÇMBT-035 VNTR HaeIII 59 GMBT-036 DU27Sib VNTR ~III 89 GMBT-039 VNTR ~III 33 GMBT-041 D23Sib VNTR ~III 81 GMBT-042 VNT~ HaeIII 78 GMBT-047 D2S2b VNTR ~III 65 GMBT-049 VNTR+PM HaeIII, Mbo 210Q5~3 WO9~/13102 PCT/US9~/0034 TABL _l (Continued) Yl~ ' ~
Name Locus1~QlYm~l~h2 Enz . 3 Het. 4 Ovin5 - GMBT-05l VNTR HaeIII 94 GMBT-053 VNTR ~aeIII 59 GMBT-058 VNTR ~I 89 ~BT-059 DUlOS2b VNTR ~HI 67 GMBT-060 VNTR+PM ~I 87 GMBT-002 PM ~3gI 37 GMBT-024 PM TaqI 62 GMBT-029 PM MboI 28 GMBT-014 DU22S2b (?) MboI, ~gI 68 GMBT-018 (?) ~3gI 17 -1 ~OCUS:locus name following HGM nomenclature rules whenever available from mapping studies.
2 POLYMORPH:type of polymorphism (VNTR:Variable Num-ber of Tandem Repeats; PM:Point Mutation; (?):un-explained).
3 ENZ:preferred restriction enzyme for its detection.
4 HET:heterozygosity within Holszteins, estimated from a sampel of 27 presumably unrelated Holstein animals.
OVIN:cross-reaction in sheep; N, negatie; M, mono-morphic; P, polymorphic, not tested.

21~0~8~

Within the American Holstein breed, the mean heterozygosity over all VNTR systems was 59.3%. When using probe G~BT-016 with MboI instead of HaeIII, and supposedly because of the presence of minisatellite variant repeats (MVR) (3~) harboring MboI sites, an extremely variable, locus-specific "midisatellite"
pattern ~36) is generated (data not shown). Used with ~boI, this probe is particularly powerful for individual identification.
We found one clear ins~ance of maternal neomutation with probe GMBT-022. Besides this, all probes showed. proper Mendelian segregation.
Table 2 reports estimated matching probabilities and exclusion powers as well. Systems GMBT-OO9, GMBT-O11 and GMBT-022 were treated as "open" systems, meaning that - because of their small size - some alleles were not detectable in our conditions. To avoid ambiguities in identification and paternity diagnosis, these unidentified alleles were pooled in a single "recessive"
class. For indi~iduals æhowing a single band, no distinction was made between homozygosity and heterozygosity based on band intensity.
Discrepancies between probe ranking according to heterozygosity versus ranking according to matching probabilities and exclusion power, most probably results from the small sample size used to estimate both types of parameters. However, heterozygote advantage at some loci could be an alternative although unlikely explanation in view of the apparent neutral behaviour of human minisatellite sequences (124).

2100a~3 WO 92/13102 P~/~tS92/~0340 TABL~ 2 ~atchi~ ~ro~ab~ liti~s for ~N~R Clon~

~me ~PRl ~p82 ~PR3 ~P~ ~PI5 GNBT-002 21.2~ 52.05 36.27 17.31 19.66 GMB~-003 2~.25 53.53 30.~0 15.37 16.56 GMBT-005 12.02 43.24 ~9.53 24.18 31.99 G~BT - 006 19.38 49.49 36.93 18.28 20.56 ~B~-007 03 ~ 25 33.38 72. ~5 34. ~7 56.19 GN~T-008 -~
G~BT-009 12.13 43.73 45.86 21. a4 30. ~2 G~BT-011 10.88 ~2.14 ~9.34 2~.34 33.2~
G~BT - 012 ~4.09 68.4~ 17.95 08.87 06.45 GMBT-013 92.28 96.28 01.96 00.84 00.16 GNBT-015 12.99 45.00 ~7.48 22.82 29.82 G~BT - 016 12.5g ~4.10 ~8~87 24.15 30.17 GMBT-017 6~.57 81.50 09.92 04.8~ 22.60 - GKBT-019 42.29 96.26 01.91 00.89 00.07 GNBT - 020 48.29 70.38 13.83 07.61 05.78 5N~T-021 21.22 50.93 35.26 17.79 19.21 GNBT - 022 02.39 32.78 75.43 37.23 61.60 G~BT-025 50.37 72.51 14.~ 07.~7 05.04 GMBT - U26 5a.10 77.26 13.16 06.39 03.11 ~MBT-028 03.19 32.60 7~ .11 36.45 58.33 GMBT - 031 20.82 50.89 36.05 17.81 20~19 GMBT - 034 71.41 84.57 Q7.72 03.74 01.~4 ~BT-035 24.2~ 53.17 32.31 15.86 ~6.80 GMBT - 036 04.19 35.42 69.74 33.07 53.05 a~BT - 039 39.79 63.90 17.83 08.65 10.16 GMB~-0~1 ~2.18 43.84 ~a.og 22.72 30.~1 GMBT-042 07.38 38.85 57.61 27.43 ~0.02 GMBT-047 30.09 5S .38 25. ~3 12.56 14.79 210û583 --~
WO92/13102 PCT/U~92/0034 T~BLB 2 ~Co~tinue~) Matchi~ Pro~ab~l~ti~ for ~N~R Clo~

Nam~ PRl MP82 p~3 ~ EPI5 G~IBT--05102.40 32.23 7C.37 38.0:L 61.37 G~Bl!--05315 . lC 44 . 7643 . 80 21. 0~ 27. 63 GI~BT--05808.2~ 40.06 55.Sg 16.56 38.03 GllBT--059 16.87 45.92 ~l0.31 19.40 2~.. 0~1 G~B~--06014.28 '~4.92 4~.23 21.78 26~89 GMBT-002 49.60 71.08 13.71 06.71 05.38 GM.BT-l32424 . 99 53 . 3630 ,. 481~, . 96 16.51 G~B!I!--029 40.2~ 61.84 18.3~ 08.75 lO.99 GMB~-01450 . 35 72 . 4306 . 3Z03 . 43 00.10 GMBT--01885.~9 92.78 03.59 01.86 00.27 __ 1 ~PR i~ M~tehing Prob~bility for two ran~omly sele~-t~ i~iduals.
2 MP8 i~ Matching Prob~bility for t~o full-8ib~.
3 ~PR i~ E~clusio~ Po~er ~h~ putati~e fath~r is unrelat~ to roal father.
~ EP8 is ~xclu~ion 2Ou~r ~h~n putat~ fath~r ~n~
r~l fath~r are fu11-8ib~.
EPl ~or EP~P~ xclusio~ Po~or ~h~n only one par~nt i~ availsbl~.

21~0~3 WO92/13102 PCTtUS92/00340 2. Ge~omi~ tri~ution: j We performed pair-wise linkage analysis between all markers. As lt is known that at least some of these sequences are organized as minisatellite clusters ~31, 35, 145), we were expecting to find tightly linked systems. We found evidence for five pairs of linked markers of which four were characterized by a recombination rate inferior to 5% (Table 3j. However, two of the systems involved in a tight linkage detect non VNTR-type polymorphisms (GMBT-014, GMBT-022). The corresponding probes were probably isolated becau~se they contain a genuine although non-polymorphic minisatellite, and were fortuitously detecting other types of polymorphi~m. Despite these five linked pairs, results of the linkage analysis pointed towards a scattering of these markers throughout the bovine genome.

Linked Svstems e1 lodscore2 GM8T-003 and GMBT-029 0.0% 5.00 GMBT-007 and GMBT-059 11.3% 9.11 GMBT-009 and GMBT-014 4~8% 3.74 GMBT-015 and GMBT-016 3.7% 27.00 GMBT-028 and GMBT-047 2.5% 9.40 1 e = reco~bination rate.
2 pair-wise lodscores were calculated with the "LINXAGE" programs.

Reference markers for the respective synteny groups were Ul:GNB1, U2:ME1, U3:NKNB, U4:MPI, U5:FOS, U6:AMYl, U7:LDHA, ~8:GNB2, U9:GPI, U10:SODl, Ull:VIM, U12:GPXl, U13:MET, U14:GSR, U15:CASK, U16:ABL, U17:CRYG, U18:GGTB2, Ul9:CAT, U20:GLOl, U21:GH, U22:AMH, W092/~3]02 2 1 0 0 ~ ~ ~ PCT/US92/00340 U23:ALDH2, U24:TG, U25:CLTLA, U26:0AT, U27:DU27Slb, U28:MBP, U~9:RBP3 and X:DMD. Synteny groups with highest concordancy scores, to which corresponding VNTRs were assigned, are underlined.
Evidence for a broad genomic distribution of our VNTRs was A;upported by the tentative assignment of 13 of them to ll di~ferent synteny groups using somatic cell hybrids (Table 4). GMBTO36 identifies a previously unmarked bovine synteny group. Probe GMBT-021 was assigned to the same synteny group as probes GMBT-015 and -016. Although the latter two probes were shown to be tightly linked, linkage ~etween those probes and the former one could be excluded for recombination rates S
15%.

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21Q~3 ~092/1310~ PCT/USg2/00340 Eight ~NTRs were as well mapped by "in situ"
hybridization: GMBT-006 to 14q~1 16, GMBT-005 to 24ql~.3-22, GMBT-011 to 26qll-21, GMBT-015 and GMBT-016 to 21q22-24, GMBT-019 to 10ql4-23, GMBT-022 to 19q21-23, GMBT~028 to 2ql3-21. Again good genomic coverage was evident, since six probes mapped to five different ~hromosomes. Probes GMBT-015 and -016 both mapped to 21q 23-24 as expected from the linkage study and the assignment on the hybrid panel. Surprisingly, five out of the eight VNTRs clearly showed an interstitial map location. Only probes GMBT-015, -016 and -022 were located proterminally, the former two identifying the same minisatellite cluster. These results seem to contrast with those of Royle et al. (35), which demonstrated preferential proterminal mapping of human VNTRs. Probe GMBT011, previously located on U26, was mapped to chromosome 26, allowing us to tentatively assign synteny group U26 to chromosome 26.

3. Con~rvation of sequ~nco ~n~ ~ap loc~tion within Bovida~:
We hybridized ten bovine VNTRs to sheep Southern blots, under slightly reduced stringency conditions.
Seven of them were yielding locus specific patterns, of which six were showing a substantial degree of poly-morphism in a sample of 5 unrelated sheep tTable 1).
Probes GMBT-016, -019 and -022, mapping in the bovine to 21q23-qter, 10ql5-q24 and l9q21-qter respectively, were mapped by in situ in sheep as well.
The three probes produced signals on chromosomes 18, 7 and 11, recognized as evolutionary homologues of bovine chromosomes 21, 10 and 19 tll6). Moreover, the signals were found over the exact positions as expected in case of conservation of chromosomal location in cattle and sheep.

2 ~ O O i g 3 WO92t13102 PCT/~'S92/00340 DI C~B8ION

To isolate bovine VNTRs, we hav~ used a strategy similar to Wong et al. ~146, 147), based on the screening of size-selected restric~ion fragments obtained by ~omplete digestion with the four-cutters MboI and HaeIII. Advantages of this strategy are: (1) the complexity of this size-range is substantially reduced; following Bishop et al. (128) and assuming an exponential distribution of restriction fragment lengths, the fragments > 2 Kb represent about 10-3 of ~he total number of MboI or HaeIII fragments, corresE~onding to approximately 104 fragments; this allows us to work readily with plasmid vectors; (2) the subsequent search for and use of the polymorphism is performed with the same enzyme used to generate the libraries, obviating the need to scr~en several restriction enzymes, hence reducing costs; t3) relying on frequent four-cu~ters, the cloned minisatellites contain very little flanking se~uences and only very few of them carry highly repeated sequences which would interfere during hybridization; (4) theoretically, the larger minisatellites targeted by this approach are more likely to be involved in mutational events and could therefore be the more polymorphic ones.
A disadvantage of this approach is the unequal representation of minisatellite loci in our library.
The libraries were generated with a mixture of DNA from 20 unrelated individuals, to increase the number of clonable microsat~llites. As a consequence, loci for which most alleles are within the selected size range will be overrepresented, compared to loci for which the majority of alleles in the population are bellow this range.
This collection of bovine VNTRs could be used for DNA based individual identification and paternity diagnosis. Combining our top S probes, matching 21~0S83 ~092/]31~ PCT/~IS92/0034() probabilities and exclusion powers at least as good as those obtained with classical systems are obtained: MPR:
8x10-8, MPS: 4x10-3, EPR: 0.999, EPS: 0.893, EP1: 0.987.
Adding more probes will of course only increase the power of the system. As a matter of fact, these probes have been used efficiently to solve pa~ernity problems beyond the power of blood group systems. DNA typing is not limited to blood samples as pr~sent systems are, which expands its spectrum of applications and power.
lo As an example, DNA typing has been used to deal efficiently with fetal blood cell chimerism (127), frequently encountered in cattle. Compared with multilocus DNA fingerprints, locus-specific VNTRs are much ea ier to interpret and are more reproducible.
Following properly established standard:ization procedures, a "common language" could be established allowing exchange of information between laboratories.
It is noteworthy that heterozygosity and allelic frequencies for some probes seem to vary substantially between breeds. As an example, probe GMBT-012 is characterized by an heterozygosity of 22% in Holsteins, but higher than 50% in both Herefords and Brown Swiss.
Hence, proper use of these probes may initially require accurate estimation of genetic variation for di~ferent breeds.
Assuming a coverage of 20 cM per marker in linkage studies, the set of markers described in this paper would allow the scanning of approximately 7 Morgans.
Accepting a total map length for the bovine genome of 25 Morgans (148), this represents close to 33~. We have complemented the set of bovine VNTR described in this paper with over 80 multisite haplotypes, generated with cosmid probes, and more than 100 microsatellite systems (31, 148, 149). Therefore, the majority of the bovine genome is now amenable to linkage scanning. Since several of these markers are already "anchored" to specific chromosomes or synteny groups, a primary bovine ~092/1310' 2 1 0 ~ S ~ 3 PCT/~ISg2/003~

DNA marker map should soon be available. Moreover, the remarkable conservation of mini~ and microsatellites within sovidae will substantially accelerate the construction of genetic maps in sheep and goats and s offar the possibility to address interesting evolutionary issues.

G~NERATION o~ BOYINg ~LTI8IT~ EA~LOTYP~8 ~BING RANDO~ CO8~ID CLONE8.

IN~ROD~TION
The possibility to generate nearly unlimited numbers of genetic markers through the study of DNA
Sequence Polymorphism ~DSP)t76~, has revolutionized human genetics: genetic markers have been used to map genes involved in a variety of human diseases, which has direct implica~ions for genetic counselling strategies and is a first step towards their sub~equent cloning by reverse genetics; they are revolutionizing individual id~ntification and the examination of familial relationships; and they are invaluable tools in the study of a wide variety of biological issues. In particular, they are expected to play a key role in the ongoing efforts to entirely map and sequence the human genome.
For breeders of domestic animal species, the availability of large numbers of genetic markers means, besides new approaches for individual identification and paternity diagnosis, the possibility to map and study genes dete~mining production traits , and to use this information in mar~er assisted selection and velogenetics (9l, 96, 141, 150).
Particularly challenging is the fact that the majority of production traits are complex, WO92/13107 2 1 o o ~ ~ 3 PCT/1S9~/00~0 multifactorial traits. Animal breeders, however, have the advantage that phenotypic information has been carefully recorded for thousands of animals over the years for use in classical biometrical breeding S programs, and that they can, if necessary, design and generate the ideal family material required for such mapping studies.
In both the human and animal field, polymorphic markers characterized by the highest possible hetero-zygosities or "Polymorphism Information Content"
(PIC)(76) is paramou~t. Hence, the focus has changed from the original diallelic Restriction Fragment Length Polymorphisms (RFLPs) to more informative systems based on the study of sequences such as minisatellite!s (18, 20), and more recently microsatellites (32-34) and the polydeoxyadenylate tract of SINE-repetitive alements (37). Minisatellite sequences in particular have proven very powerful. They seem to suffer, however, from a non-random genomic distribution, especially in the human where in addition, they show proterminal confinement (35). Microsatellites, although very abundant and highly polymorphic, require prior sequencing e~forts to generate the primers needed for their in vitro amplification. Moreover, the large scale use of microsatellites requires the development of more efficient multiplex amplification and data collection schemes.
An alternative strategy for the generation of highly informative marker systems is to combine several, closely spaced diallelic RFLPs into more informative polyallelic multisite haplotypes (98). We have explored the use of random bovine cosmid clones in Southern blot hybridizations in order to identify such sets of closely spaced DNA Sequence Polymorphisms. Because of the population structure imposed by breeding strategies, effective population sizes of domestic species are expected to be reduced compared to humans. It was WO92/1310' 2 1 ~ O 5 8 3 PCT/~92/00340 interesting therefore, to check in how far this would decrease the observed level of genetic variation and in how far the expeoted concQmitant increase in lin~age disequilibrium would affect the efficiency of the chosen approach in domestic animal populations. -MA~IA$~ ~ND ~OD~

.
l. Pr~par~io~ of ~o~id clon~
Bo~ine genomic DNA was prepared using stændard procedures, partially digested with MboI, and size fractionated by rate zonal centrifugation in a lO%-~0%
sucrose gradient. Size fractions around 40 Xb were ligated into the XhoI site of the cosmid vector pWEC
~pWE 15 vec~or (Stratagene) with pUCl8 polylinker -Erica Cumlin, personal communication), after partial fill-in of the insert and vector sticky ends with respectively dATP, dGTP and dCTP, dTTP. The obtained constructs were packaged into Gigapack II Gold extracts (Stratagene) and used to infect E.Coli 490A hosts (gift from R. White, University of Utah, Salt Lake City, Utah, USA). llO colonies were selected at random, cosmid DNA
was prepared using standard proced-lres, and purified by CsCl/Ethidium Bromide isopycnic centrifugation.

2. ~outhern blot hy~r~isatio~:
Genomic DNA from 9 unrelated Holstein individuals was prepared from venous blood using standard procedures and digested with 5U/~g of the following enzymes in the presence of 4mM spermidine: BamHI, BglI, BglII, EcoRI, EcoRV, HindIII, Xpnl, MspI, PstI, PvuII, TaqI and XbaI.
4~g DNA per individual was separated according to size by agarose gel electrophoresis and blotted onto Pall Biodyne B membranes using NaOH 0.4M as transfer buffer.
Membranes were prehybridiged at 65 C for 4 hours in 10%
PEG, 7%SDS, 50mM NaHPO4 (pH 7.2) in the presence of 350~g/ml bovine genomic DN~. Cosmid DNA was labelled by ~VO92/13102 ~ 1 0 ~ 5 ~ 3 PCT/~1~92/0034~

random-priming (111) to specific activities of .5*109 cpm/~g, prehybridized with bo~ine genomic DNA (5mg/ml) for 90 min. at 68 C (112), and added to the prehybridized membxanes for 16 hours. Final washes were in O.lXSSC, 0.1%SDS and at 65 C. Autoradiography was carried out for 2 ~o 6 days at - 80 C with Xodak XAR-5 film and intensifying screens ~DuPont Cronex Lightning-Plus). Membranes were stripped by boiling into 0.1%SDS
and reused up to at least 10 successive times.

3. Calculatio~ of ~ucleoti~ r~iti~:
Nucleotide diversities, ~, corresponding 1:o the average heterozygosity per nucleotide site were estimated following Ewens et al. (130), using:
~lk ~ =
2~rlml1nni where ni stands for the number of chromosomes studied with the ith enzyme, rl for the number of bp of the recognition sequence of the i~ enzyme, m~ for the number of cleavage sites explored with that enzyme, of which k are polymorphic.
The number of explored restriction sites was estimated from the number of fragments f observed by Southern blotting, using m = (3f+1)/2 (119).

RE8~T~

110 randomly selected bovine cosmid clones were usad in Southern blot hybridization experiments as described in Materials and Methods. 96 of them, or 87.2%, gave usable patterns and were kept for further analysis. Combining data from the 12 restriction enzymes used, a mean of 53.87 fragments per cosmid WO92/13102 210 0 5 8 a PCT/~1Sg2/00340 qualified as unambiguously readable. The only RFLPs considered in this paper, are the ones affecting these selected fragments.
Assuming a cssmid insert size of 40 Xb and estimating the mean restriction fragment length in Kb (L) for a restriction enzyme according to Bishop et al.
(128), the expected number of restriction fragments detected in Southern blot hybridizatlon per cosmid probe for a given enzyme can be approximated by:
integer(40/L)+l. For our 12 enzymes, we expect therefore a total of 173 fragments per cosmid probe.
Therefore, the 53.87 fragments actually observed per clone, represent only 31% or less than l~3 of what is theoretically possible. The remaining 69~ are missed either because they were considered difficult to read, or more often because they went undetected due to their abundance in highly repetitive elements blocked by the competitor DNA, or due to their size, too small for efficient detection in our conditions of Southern blot hybridization. The smallest fragments qualifying as readable in this study, were in the 1 Kb size-range.
The latter factor is particularly apparent with the two used four-cutters, MspI and TaqI, whose expected mean fragment length are the lowest (1747bp and 1179 bp respectively) despite the presence of the rare CpG
dinucleotide. Only about lS% of the expec~ed number of fragments are detected for these two enzymes.
Nevertheless, as much as 82 of these 96 cosmids, or /85%, were showing at least one polymorphism within our sample of 9 randomly selected individuals. The detected polymorphic events are classified into two groups: l) Point Mutations ("PM"), whenever a defined polymorphic pattern is only seen with a single enzyme, and 2) Insertions-Deletions ("ID"), whenever such a pattern is seen with two or more enzymes. Followin~ these rules, we identified 215 polymorphic events, or a mean of 2.6 independent RFLPs per cosmid probe. 162 of these qr~ ," .", J ,/~ ." ~ "~ ", " ,,~ ,, , ~,, ,, ~, ~ ", , , , ",,~ , ,, , ,, " ,, ,, ~ ~

~:) 92/1310~ 2 1 0 0 ~ 8 '-~ PC~/US92/00340 --6 l--(75.3%) were considPred of the PM type, the remaining 53 ~24 . 7%) of the ID type. Table 5 summarizes these results .

W092/l310~ ~lao j8~ PCI/US92/00340--:
Bovine Multisite Haploty~es N~,L Ba~HI Bgll BglII Eco~! ~coRV
============ ========= --=======~============= =============== =========== ===========
115BTOQ! IID~l!) ?13 _ _ ____ _ _ ____ _ __ ____ _____ ____ _____ _ _ __ _ ~S~T005 2P~.~5i lID(17i llD~17~
617 4.2t2.913.9i3.2 q.5+1.519.!tl.6 3P~ ~Si __ ________ ________ _ _ ____________ _ __ __ ________ _ _ _____ _ ___ ___ _ ~SBtO''7 lID;17i 31~
______ ____________ _ __ ______________ ____ _ ___ _ ______ __ _ _____ ___ tlSBT005 ___ _______ _______ _ ____ ________ _______________________ _ ____ _ ___ ~SE T 03~ IPY, ~2Pv~
'!/5. e "52'0:1 31D~39i IP!i~22) 7.~15 7.71S
2P~ 9 - 5.51~
_ _ ___ ___ ___ ____ __ __ ____ _ ______ __ _ ____ _ _ __ ___ _ ____ ~SBT013 1 !D ~22i 9.5110 2P~'.(6) ?13. 7 ______________ __ ____________________________ ______ __ _________ _ _ __________ H58T015 lID~4~) 2PH~23) lID~44i 14113 2512~ e.5/6.5 , __.. ________ _ ____________ ___ ___________ _ ______ __________ ____ _ _________ .
IlS8T016 _ _ ________ _ ____ _ _ _ ___.__ _ _ _ _ _ ___ _____ _ __ ~IS8T017 -WO 9~/13~02 21~ 0 ~ ~ 3 PCr/l'S9~/00340 TABLE 5 (Continued) Y~E Hindlll K~nl 'Ispl Psti PYII
= = ======= = ~ === = = = = ============ ==== ====== = I
~ssTclq ??(??) IP1~22 20/ 17t2~ 8 2Pr,~7) 30~5. 5136 .
~SBT020 IP".~' !) .315.5 _____ _ _ _ _ _ __ __ _ _ _ ~ __ ~S~T00 1 21D ~S ) ; . D ~11 ) ?? ~ ?? ) 9112.5 ?18.8 55) 12/12.5 ________________ _ ___ ____ __ _ _ _ _ _ _ _ _ ..
~SaTOOS llg(17) IID(17) 11~17) S~ 8~5/5~ ~t4~ B
~P~ (50) ~l7 . 8 ___________________ ___ _ _ ___ ____ _ _ _ _ _ _ __ ____ __ _ !~SBT007 lID(17) 1iD~l7) 12tb.~ 8.219.5 _____________________ __ __ _ __ _ _______ __ ______ _ _ _____ _ _ ___ ~SDTOO9 lPH~44) 2Pllt~b) 3,~15,7 4.5~5.119.5 _________________ ____ __________ _______ __________ _______ ___ _ _____________ !~S~TO0~ 2P" ~28) 51~. 5 3P~!lll) 3.812.B
4P~1 ~5) 1115.3~!.6 _______ ___________ ________ _______________________ _ ____ ____________ _________ __ ~S~~011 ~.P~''2) __ ____ _______ ______________ _ _ _______ _____ ______ __ __ _____ _____ __ IISBT013 lID~22) ?9(?q) l9tl6 ________ __ ----__ _____ __ ___________ ______ _ __ 210 0 ~ ~ 3 WO 92~3102 PCI/I~'S92/0034 ~64 -LE 5 (Continue~) ~a!l~ HindIII Kpnl ~Ispl Pstl Pvull 1~.5~T015 ~?t??l .SBTOI~ 1 ID t~0) 5. 9~ . 31~. 5 ~s3TOI7 ~!SBT019 lP4t501 ?? t?~) lI/S.~+4.3 _ _ ~SBT020 2P'1~221 ______________________ ________ __ __ __ _ __ __ _ ___ _ _ ______ ___ _ _________ ~0 92tl3102 210 0 3 0 3 P~/l,1S92/0034û

T~E~LE 5 (Continued) ~M.~E Taq I XbaI HET . ~, =====:==============__==5==_===__=:=__=5___==== i ~SaTOO; ??(??) 55 ~ISBT001 0 ~S~T005 1 ID ( 17 ~ 5 4. 2t2. 213. ~+2 ~!SBT00~ 7P"(5) 0 _____________.______ ___________________ I~SBT007 _ ________ __ _ __ ___ ___ __ _ ___ ~SBTOOq 77 ______ _____ _____ _~_ ___ _ ______ ~!SBTOOa SG

~S~TOOp O
~S~TOOP O
________ ___________________________________ r.S~T011 3!D~39~ ~7 ', 3t21 ~IS~T01 1 0 ~, ~t4, bt2 . q _ _ _ _ _ _ _ _ ~ _ _ _ _ _ _ _ _ _ _ _ _ _ ~S~T013 lID(22) 33 e.,S/~
t!STOI3 __ ________ _______________________________ ~IS~T015 IID~U) ~6 I~S~TOIS 3P~(33) e.3110.8 ____________ ____________________________ ~S~T016 110(~0) 50 6/3.3t2. 5 _ _ _ ______ _ _ __ __ _ __ __________ nSBT017 _____ __ __ __ ___ IlSOTOlq 4P~t~U) 09 11S~TOI9 sPr~3gJ o -I~S~T020 3D~35~~6 ______ ______ WO 92/13102 21~ 0 ~ 8 ~ PCI /l 'S92iO0340 TA~3LE 5 (Continue~

N~. r3a~HI Bgll ~3glII Eco~I EroRV
_==_=:==_=_========__:===__===_=_============:======_=:_==_===5_=======_===_===========
r,SaTo22 ~Pr~7) 2Pr,(~7~
3.313.5 5. 6/2.~3.2 3P~1 (5) 5 . 8 1 9 .
1 3 ) ?/2.1 ___ _ _ _________~_ __ _ _ _ __ ____ ________ ___ ______ _ _ __ __ ~S9T023 ___ __________ __ __ __ _ ____ _ __ _ __ ____ ____ ___ __ __ ___ ______ ___ llS3~T024 llD126) 1ID~263 14/7.~ 10110.7 __ _ _ _ __ .
!~S3T025 lPn l 1 1 ) 2P~3 ~33) 9.617.5+~.2 1219.7 ______________ ____ _ __ __ ________ _________________ __ ____ _ __ ___ ~IS~T026 199(??1 I??t??) 81?
_ ___ _ __ _ _ _ _ _ _ __ _ _ _ __ _ I~S9T32e l!D(37) li8~3~) liDt37) IID1373 5/6.3 1~110 1~16.6 6.1~5.
21D(??1 21Dt??) 21DI??) 31? 15/? 3.~tS/?
_ ~ _____ ___ _______ ___ _________________ __ _______ _________ _____ ____ __ _ _ ~SET03C lPP.16, 3 8 / 5 . t3 t, ________________________________________________________.._________________________ t~lSET031 ?? ~??) __ __ _ _ __ _ _ _ __ _ _ _ __ _ _ ___ _ ~_ ~51~3T032 lpr~l22~ 21D(11) 2ID1113 9.b~6116 ?/6.2+7~12 ?/6.1 ___ ___ _______ ____ __ ___ ~SBT033 lP~3(11) 2IDt U ) 3.515 ~/7.8 2~ ?? (??) 5. 91 ~P~ ~4 9~a2 3T03~

~, ~,, ,.. . .. . . ~ ... . . . . . .. . .

~;40 92/13102 2 1 ~ 0 5 ~ ~ P~ S92/00340 TABLE S (Continued) N~lE HindlII Kpnl tl5pl Pstl Pvull ~ISBT022 ~.

_____________ __________________ ___ _________ ______ _________ _______ __ , ~SBT023 lP1~1~51 5. 5t3, 713 . 2~2 ________ __ ___ _ ___ ___ __ _ _ _ _ __ __ ___ ______ _ _ _ _ ~SBT024 11~2b) llD12b) llDl2~t ?f7.~ 7.5/5 1311~

______ ______ _ __ ___ _ _______.~______ _ _ __ _ __ ____ __ _____ nSel025 ??~??) _ _______ _________ _ ____ ______ _____________ ______ _ __ __ _ _ ___ ___ __ ~S~T026 ? 2??~n.?~

~ ______ ~___ ~ _ ___________________________________________________________________ NSaT02~
_____~_____ _ ___ _______ _ _ __________ _____ __ _ ____ _____ __ ___ ___ _ ~S~T030 3??1??1 3. 313.
__._.___ ~___ _ _ _ _________________ ___~_______ _______ __~____ ___ __ _ nSBT031 ??1??) ?7(??t ??1??) ________________ __ ___ __ _______ ____________ _ _ ______ ____ __ ________ _ ~SBT032 2IDlll) 2I011') ?/12 ?12.7 3P1t(33~
. 9 41D~5 ~13. 4 ___________ __ _____ __ ___ _____ ____ ____ _____ ~
l~S~T033 2I~4) . ~1Dt6b~ 4IDl~b) ??1??) 1~115 ~.51~.5 ~.71~.3 ?? l??) _ ___________ _ __ _ ________ _ _ _ _ _ ___ _______ _ __ nS~T93~ lIDl4~) ??1??) 110~4~) 5.913.9 ~.71~.3 2Pn(20 5.3l~.
_~___ ____________ _ W O 92/1310' 2 1 0 0 5 g ~ PC~r/ ~'S92/00340 TAB L E 5 ( C o n t i n u e d ) q~ Taql Xbal HET.
= =====~====== === = ===== ================== . , ~SaT022 SP~44) 89 ?13.2 ~SgT022 ??1~?) o nssTo22 HSBT023 2P~1331 ~5 _ _ ~SBT024 IID~261 ilD126) 26 1~/15 3.8/~.7 ~SaT024 ??1??1 0 _ _ _ ~53T025 3P~(371 78 ~S~T0"5 4P~1??) 0 _ _ ~59T02~

~SBT02B 37 r.saTo2a ~S9T030 2Pn~ll) 7 5J5.2 ~SBTC~: ??l??) 0 _ r~seTc32 ~ 5~ 67 4.1/~.3 ~SBT032 __ _~ _ ~S9t033 5P~IU) 21DIU) BB
~13.~ S1~.~
~S~T033 o 4?t??~
~SBT03~ o 1034 IIDI~) 56 5.~1~
HSBT03~ 3??(??) 0 ~/0 92~1310~ 2 1 ~ ~ ~ 8 3 p~ 9~/00340 TABLE 5 (continued) i N~rl Ba~ 911 a91 I I Ec~RI Bco~
~__====_2========:_:_=====::==-:==-:==:-=-===-=:====::__=__===_=_=_======_=_=====~===__ ~
r~s9To35 llD~55~ ;
6.31b.b ~`
_____ ________________ ~___ _ _____ _____ ____ ____ _ ______ _____ _~_ _____ ____ ~SBT037 3??111 i 2??111~

__ ._ __ __ __.. ____ ____~___________________ _ ___ _________ _ __ _ ~58T03~ 5~1??) IID(Ill IIDtlll lID(ll~ IIC(I11 6.8rl0.s IS~o/l~.o 7.51b.4 7.516.

_ __________ _______r_____ ____ __________________________________________________~_ r~S8T03q l~n 919. b ____ _______ ___ ___ ___________ ___ _________________ __________ _____ __ ___ tlS8T040 1~ 22) lB/lb ________ _ _ ___ _________ ___ ______ ___ ___ __________ _ _ _ __ _ _ _ __ _ _ _______________________ __ __ ________ _ ____ __ _ tlSET042 lPr5~33 2P~ 3 6l~
_____ _________ __ __ _____________________ _ _ ____________ ____ _ ___ ______ _ r~ssTo43 ?? ~9?) l lD 1~1 1 ID (44~
.51~.t 3.213.6 21D ~551 3.7l~.3 _ __ _ ____ ___ _ ____ ________________ _ ______ _ ____________ __ _ _ _ _ ~59tO~4 _________ _ ______ _. . _______ .____ ____________ _ ~SET045 lID(U) 2ID~B9~ 2ID(B91 ~13.~ 3.9/6.513.5 ~ 13.5 _, ______ ____.. ______ ___ _ _____________ ____ ________ __ ~ _ __ __ 1158T0~ llD~781 IID(781 3.5J3.~ ~.5l3.

__________ _ _ _____ _ ____ _____ - .__ _ _ ~S8T0~7 IP11~111 ~.317.3 2Pn(3o ?/10.5 2131~.~83 ~
WO 92/13102 _ 7 0_ PCg /US92/00340 TAB L E 5 ( Cont inued ) Ha"S .~indlII Kpnl ~spl P~tl Pvu!!
=-==========5==_==~==_=_:2_===============_==_====_=====_====_===_===_=================
~SBT035 21Dl??) 218(??) !158T037 IP!~ t2~ ) 4. 515 ~?? (?? ) 3. 81?
_ ________ _ ___ _ _________________ _____ ___ _ ___.,.__________________.. ____ _ ~SaT038 lIDtll) IID(ll) 2P~137) IID(II) 9.~1~.5 ~.0/5.5 19/2.7+1~ ~t~/2 ~?? t??
4.31?
_____ __________________________ _ _______________ __ ______________ __ ______ ~55T035 _ _ _ _ ..
r,SBT040 2?? t??) ____________________ __ _ _ __________ _______ ___ ______ __ __ __ _ __ __ __ ~!S~tO41 _____________________ ___ __ ____ _ _ ________ __ _ _____ ___ _____ _ _ _____ P.SBT0~2 3P~ (U) ?? (??~
71?
_ ______ ____ _ _ _ ________ _ _________ __________ __ ______________ ____ ____ ~IS3T043 llD1U) SPI~(33~ blBt33~
6.516.9 2.9/3.1 ?12t2. 1 3P~1~7e) 63~(30 7P11(10 . 813. 3 3. 713. q ~12, 4 ~P1~17~) .~14.
qP~ l??) _,,__ _______ _______ _ ________ __ ______________ ___ _ _ _ _________ __________ ~53T044 O II~t??) 3. B513. 913,~5,, _._________________ ___________________ ______ _____________ ________ 1~59T045 lIDi~4) llDlM) , . 11/10.5 ____ __ _ _________________ __ ~__ t~SBT046 __ ___________ . _____ ~5~0~7 3Ptl11~) b. St2. B/q. 3 4P~ 1??~

21~0^J8~ ' ~0 92/13102 -7 1- PCI/US92/~0340 i TABLE S (Continued) I

NA~.- TaqI X~a~ HET.
==================s=-=-=s===========
~S3T035 llD(55) 55 7.al5.3 _ ~______ _ _________ ___ __ ___________ I
~53T037 22 ~5aT037 0 __ __________ _______ ______ _ ___.. _______ .
~ISBT038 lIDIll) lIDtll) ~4 5.51? ~.~15. B
!~53T033 3P`';t22) 6??(??) 0 3 . 5l?
_ __ _ ___ ___ _. ____ __ ___ _ I~SgT0~9 1 1 __ _____ __________ _ __ __ _______ ___ I'.S~T040 22 ~_ _ _ _ _ _ __ nsaTo41 o _____ ____ _ _ _ _ _ _ _ _ ~SBT042 ??~??~ ??(??~ 44 IlSaT0~2 __ __ _ _ _ . _ 115aT0~3 8Ptl t ! ! ) 9q 1.3t2.9 I~â3T043 2ID(55~ .
1.~12.2 ~IS~T0~3 0 ~SB, 043 _______________ _ ___ _ ___ _ ~SBT04~ 0 _______ ______ IISBT045 3Pt~33) ~9 ~___ __ IlS~tO4b 2Ptlt33) 7B
~.915.2 t~S~T0~6 lI017B) O
2.9/1.7 ~________ ___ ______ ____ l~aT0~7 5P~??) U
1~/21 t~S8T0~1 wo g2/l3ln2 72~l ~ O a 8 3 P C~r/ U S 92/0034~

T A B L E 5 ( C o n t i n u e d 3 N~' D~HI ~9ll BglII EcoRI EcoR~
~5~0~8 ______ _ __________ _ _ __ _____ __ ________.. ___________ ________ ____ _ ___ _ ____ ~59T04q lID~22~ lID~22) ql3.9~5.3 S.71~
_____ _ _ __ ___ __~ _____ _ _ _ __ _____ _ _ ___ ____ ____ .SBT050 IP~(??~
?J9.2 __ _ _____ ________ _____ ______________ ___ __ ___ ________ ______ ___ ___ _____.~
~S~051 2P~22) lP~
20~16 7~1 __________ _ ____ ___________ ____________..______ _ ____ _ _____ ____ ____ ______ ~SBT052 IID(Il) 21D~??) 1711~
_ _ ____ ____ _ . __ _ ______ _ __ _ ___________ ___________ _ __ __ _ ..
~SBT053 lP~t??) ______________ ___ __ __ ___ _ ______ _ ___ _ _ _ __ _ ________ __ ___ ___ _ ~Issross - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -~SET056 llD~55) ~ID~5') 113~S5 5.~17 5.7t7.
2P~22) 12/S.5 _______ _ __ ___ ___ _ ___________ _ _______ _ __ ________ ____ ____ __ _ ~S~T057 llD~ll) IID~Il) ?/B ?1e.5 2P~??) 716.7 ____________ ______ ___________ ____ __ __________ _____ _ _____ _ _ ________ ~SBtO5~ 11Dt33) 110~33) 1.8/3.~ ?13.2 ___ _____________ _ _______________ ____ __.___ ._ _ _ _______ ~53T060 llD~Il) llD~ IID~II) IID~II) 6tS.B 516.5 q.6111 q.6/11.5 21~15S) 2tD(551 6.~1q.6 3.516.6 3P~(22~
'1.~/B.e ____ _ ______ _ __ ~SBT061 2101~5~
~V O 92/1 31~2 P(~r/ U S 92/00 TABLE S (C o n t i n u e d ) Hin~IlI Kpnl ~spl Pstl PvulI
~SBT0~8 lID1~4~ 1Igl~4) lIDt44) lIDlU ) 6.21s,qt3 27115t7,~ 4,3/3,7t3,2 b.51q 2P~
5.514.6 ~SBT049 2P~(11) 3P~(9?~

8.41?
~SBtO50 2P~t??~ 21D133) 3P~111) 2ID~33) ?/l,S 3.2/2,B 2tl.bl6.8tl.e _ _ _ _ .
~53T051 3P~(??) ~S3T052 llD(??) lIDIIl) 12/10.5 _ ~S8T053 21D( U ) 2IDl44 ?16.~ 2.1/2.
3P~(??~
11,5111 ~: llSaT054 IPt~122~
2~ 9 ----------------------------------------------------------------------------------------------___----____________________________ ~S~T056 IIDl'5 31~.7 :~ _ _ .............. ..
~ ~SBT057 ;

:

_ _ ___ _ ~58tO59 1IDl33) !:. I 12/11 -- _ _ _ _ _ _ ___ ~53T060 lID~ 2p~li?~

~S~T0~1 lIDt U ) 417/4542 WO 92/1310~ _ 7 42 1 ~ U ~ 8 3 P~/~lS92/0~34~ -TABLE 5 (cQntinued) NA.~E TaqT Xba! ~ET.
==============_========= === _====_,.:==_===_=
I~S~T0~9 11 D ~ U ) 55 6. ~17 ~SBT048 0 __ _ ________________ _ _ ___ _ __ ___ I~SBT04q 22 _ _ _ _ _ _ _ _ _ _ _ _ _ ~ _ _ _ _ _ _ _ _ _ ~!SBT050 2ID~33~ 55 2. 5t2, 71~, 6t2~ 9 _ _________ ______ _____ _____________ ~S~T051 ~P~1122) 78 5.2/6 ~SBT051 5P,'1~33) 3. 3/3. 1 __ __________.. __ _________ __ ______ llSBT052 lI~ll) 11 E17. 8 __ __________________ _______ _ _______ P.53T053 ?? U
~SBT053 0 __ ___~ __ ________ __ ______ ________ I~SRT05~ 2P!~(11) 3PI~33) 33 l.b~7.l 3.7/~.E
___ ___ _____ ______ __ ______ _ __ ____ ~SBT056 IID(55) ~6 L.5t4.7 ~ISBT05~
__ _ _______________ _______ _____ ___ _ r.SBTû57 ~P~lll) 4P~l(U) ~6 l~lq.5 20tl5.5+~.5 ~SBT057 __ _ __~__ _______ ___ _________________ t~SBT05q 2P~1 t66 ~ 66 7.81~.~
llSBT05q 3Ptlt??) 0 _________ IISBT060 b6 llSaTO~O o IIS~TO~O O
-IISBT0~1 U

2 1 ~
WO 92/1 31 0~ _ 7 5 _ P~T/U~92/0~340 T AE~ L E 5 ( Cont inued ) NA~ Ba~HI ~9ll Bglll E~o~I EccRV
1~53T062 ??1?~) ____ ___ ___________ ____ __ _ _ _ ____ _ ___ _ __ __ _ _ .
~SBT064 ~S~T065 1 ID 1~4) .5l7 ____ _______ _ _ ___ ____ ___ _______ __ ____ _ __ _ ____ _ ?!S~T0~7 1 ID 155 ) ~17.3 _______~___ _________~__ __ _ _______ _ _________ __ _ _ _ __ ____ _ _ ~SBTObe IID(11) lID(111IID(11) lBlll 1~/12 10/8.8 __. _______ _____ __ _____ __ __ _ ____ __ _ __ _____ ___ ___ I~SBT069 2P~1331 lPI~(~4) 3P~I(ll)~Pn(22) l~i3.5/lq.5 12.5/10.5 ?l9 ql3.4 .
__~_____ _________ _____ __ _ _ _ __ _ _____ _ ___ __ _ _ _ _ _ _ _ ~159T070 IP~.l!l) 21D(55~ 2ID155l 2.512.q 2.7/1.2 _ ___ ____ __ __________ ___ ____ __________ __ __ ___ _ _____ _ _______ ~ ___ ~ISBT071 _____ ______________ _ _________________________________ ___ ______ _ __________ ~SBT072 lID1331 ___ _ ____________ ___ __ _ ______ _ __ _______ _ _ ______ __ ___ _ ____ __ ~5BTo74 ~SBT075 _______ _ ______ _ ___ ___ ______ _ _ ______________ __ ____ _ __ ___ ___ _____ _ ~SBT076 2iDi~4) lID133! 2IDlU) 2}D14~) 6.2/4.B 2.512 212.8 2.3/2.q _ . ______ ___________ ___________ ______ ___________ tlSBT07a IP~(ll) 2P~I11) 7.616.7 7.81b.7 _____ ___ __ _______ _ ~ ______ _ _ _ ~ _______ _ _ _ ~ __ ~SaT079 ??~??) __~_ ~ __~ _ __ ~59TOBO lIDI11) .5~2l5.7~.3 __ _ ______ ___ tlS8TOS1 ??(??) ??1??1 ?lll 21~0a~3 W O ~2/1310~ PC~r/ U S~2/00340 TAB LE 5 ( C ~ n t i n u e d ~
N~'l. Hind!II KDnI l~spl PstI PYuII
_==:===:==__==--======:=_==::=====_==:=====_===:====:_==5_==_==_==_====2=5=-_=========_ ~,5~1062 iT06~ IP~(3~) ~5/1~
___ _ ___________ ___ __ _ _ ___ _ _____________ ___ _______ _ ______ ______ ____ _ ns~To65 lID~4$) 5.2/~
~SaT06~ lID~55) 3.q13.5 ~ _ _~ _ _ _ _ _ __ IlSaTObE
_ _ _ _ _ _ ~SaT0~9 sp~(33 ?13.2 _ ~S~T070 3P.~22) 4P~111 sPrl~ç~) ,~t~.s 13/10.7+2 8.7J9.S
~SET071 lP~?~) ?18 __ _ _ _ _ nSBT072 2P~(ll) lID133~ 3P~??) ~D~
q.518.2 3.71~ ?15.4 5.9/9 ~SBT074 lP~(22) 2PY144) 5~ 33;.8~5.3 lO.a/1~19.5 3,3/5,~
3Pn~33) 17/16.5 ~P~44) 10.81121q.5 ~ _ tl5aT075 _ __ _ _ _ _ ItSBT076 _ ____ _____ _ -- _ _ -- __ _ ~SBT078 3P~
?Ib ____________. .__________ _____ ____ __________ I~SBT079 ~__________ _ ___ nS~T080 IlDtll~ IID(I1) 2J2.6t2.7 Il/~t~.a ________ ___ __ ____ ____ _ ______ tlS~TOal 2P~33~
2.212.7 ~V O 92/1 ~02 2 1 0 ~ 5 ~ 3 P C~r/lJ~ 92/00340 TABL~ 5 ( C o n t i n u e d ) N~.~ T2qi X~a! H~
========= ==== ====== ==== ==== == ==== == ===
~ISBtO62 ??(??) 0 _ _ ______ _ _____ .,_ __ _ _____ _ ~
~saTo~
~S~T065 lID~)llD~4~) ~4 6.71~.7~.~13. 1 ___ _ ________________________ ___ _ ___ ___ ~SBT067 2P~??1 55 __ .SBT068 2P~33) 4 311.6 _ _ ~SBT06q 8q H53T07C 6P~??) 7 ?13.7 _ ~S~T07! 2P~111) 3P~t??) 11 1~/11 .SBT0725P~.(111 7E

_ ~SaT074 66 ~SaT07~
_ _ --~SgT075 IP~
3.21~.4 .
nS~T076 lID~33) 213.8 ____ _ _ _ _ __ ~SBT07a 4P~33) ~

_ _ __ _ __ _. _ HSBT07q IP~ll) 4 ~S~T07q 2Pn~33) 0 3.513.7 __ __ __ . _ ___ __ ~SBT980 2P~ 22 6.~13.~t2.~
_____ ___ ~58T081 33 W 0 92/1~102 210 0 3 8 3 PC~r/ ~IS92/00340 T~ L ~` S t C o n t i n u e d ) J
~,4Y-- a,~-~,! Bgll ~glII EcoRI EccRY
======== ==========,= ===== = ============ == = === ====~====== == ===== ====== ==
_ ,Y,S9T083 2P,Y.1$4~ lP~,(11) 3IDt33) 3ID(33) 11117 ?t$.5 513.8 5.513,9 _ ~ _ ~SBtO,9~1 IP~U) . 519 ___ ____ ___________________ _ __ ________ ___ _ _____ ~s'BTOB5 lID144) 31D155) 7.71S.5 5.51~.9 2iD(22) 2ID122) 3.31~.8 ~ -5 ,n.S~TOB~ 21D122) IP~(Il) 3P.~111 10.5+2.2/12.5 6.216tY.4 ?13.7 _ _ _. _ rSBTO9?

~s8Toe8 _ _ _ _ I~SaT~E9 ~SaTO90 __ _ _ ~
~S8TOql ??(??) 5.51~
___ _ ~S~T092 . _ _ .. _ ~SPT093 ~ _ _______ , ~IS~TOq~
____ _ _ _ _ _ __ _ _ _ _ ..
~SDT096 __ ____ _~_ ~S~T097 ~S~T~98 ~0 92/~3102 2 1 ~ 3 P~/lJS92/0~34~) TAsLE 5 (Continued) N~- NindIlI KpnI t!spl Pstl Pvull ====__=_==_==_===_===_=====_=====5_====_===_===_==_==================_==_=___====_=_===
______ ________________________________________________________________._______________ .
~SBTOB3 4Pt~(~?) ?17 _ _ ___ ______ ___ _ _____ ______ ____________ _______________ _ __ ___ ____ ____ ~S~TQ8~ ~Pr,~22~
S.113.q _______ __ _ _____ _______ __ _______ __ _ __ ______ _ ___ ___ __ __ ~tSBT08S 2ID~22) 12/'. 7 ____~_~ _______ ____ ___ _~ _ _ ___________ ________ ___ _ __ __~_ _ __ I~SaT086 2ID (22) ~ID (55) 5P~1(55) 18121 2. 515 7. 1 /5 ~ID (55) . 3/7 __________ ___ _______ _ ____ _______ __ _ ______ _ _____ _ _ _ _ _ __ _ __ _ tlS8TOB7 __ _ _ _ _ ______ ___ __ ________ _ ___ ______________ __ _______ __ ___ ___ tiSBT08B

______________ __ __ ______ __ _______.. ____ ___ __ ______ _______ _______ _ tlS~TO~9 _ _____________________ ______ ______________ __________________ _ ________ ____ ~tS8TOqO

___ _____ _ ______ ___________ _ _ _ __ _ ___ __ ______ ___ _ _ _ _ _ ____ ~58TO91 ?~??) 3ID~33) ?I;
______________ ________ ________ __ ___ ________ __________________ _ _ __ ~58TOq2 lPtl~4~ 2P~1(22) 4.3/2.B - 3.51~
______________._ ___ _ __ ______ _____________________ __ _____ ____ _ __ ~ISaT093 IP~I ~331 ~,7/1.9t2,5 2P~1 (33 ?13.3 __ __ _ _______.___ ____ _______ ~IS3TO9~
________ ' ___ ___ _ __ IlS8T09b IP~I ( 11 ) ~1~. 9~3. 2 __________ _ _ _____ 155DrO97 IP~I(??) ~ __~ __ _ I~SBTO9~ IP~1(66) 2P~(??) WO 92/ 1 ~1 0~ - 8 0 - PCl /I~'Sg2/00340 TAB L E 5 ( Continued ) -~E T~qr XbaI UET.
===~=:~====~=========a=--=-=======_=__====_===_ ______________________________________________ ~SBT083 SP~ll) 66 ?1 ________ __________ __ __ ______________ ~S9T03~ 3P~t33) 55 2.3J2.~
~SBTOB4 ~P~??) O
?/2.8 _____ _____ __ _ ________ _ __ ____ ~S3T05 3ID(55) 2ID(27) 55 513.~ 11+15/23 ~S9T085 lID(U) O
?12.7 ______________________________________ r __ ~SBTOB6 ~iD(55) 76 613.3 ~SBT086 O
___ ____________ _ __~ ____ ___ _______ _ _ tlS3TO~

~SBTO08 o _ __ _ _ _ ~S~T06q O
~SBTO90 lPn I ~
1.813.5 ____ ____________~__ _ _ ______ _____ ____ ~B~TOql 31D~33) 33 ?15.9 ___ ___________ ___ ___ __ _______ ____ ~SBT09X 3P~(33) b6 5.31b.8 ____ __0__________7_______ ______________ _ saToq3 u ~saToq3 0 ____~____ ~SBT09~ o ~S~T09b 11 ~SBT097 2P~l??) O
_,_ ~_ ~SBT09& ~P~lSSt SP~lllt 66 .S/I 18115 WO 92/13102 ~ 1 0 0 ':J 8 ~ PCr/US92/00340 i .
T A B L E 5 ( Cont i nued ) !~YS ~a~HI ~g!i ~g!lI Eco~l EcoRV
~S~TOS
________ ___ _____ ____ _~_____ __ _____ _ __ __ ___ _____ __ _ ___ i ~S-~TOqq IP~t(331 2'`Dlll) 2ID~
1~/15 10.5t~l15+~.9 ~+418.e+3.9 __ __ _ ______ ____ _ _ _ __ _____ ___ __ ____ _ ~158T100 __ _______ ___ _____ _ _ _ ___ ____ __ _____ ~ _ _ _________ _ __ _ ___ __ IIS~T101 2P~(221 IP~ U~
~/10.2 ~t7/16 _______ _______ ___ __ ______ _ __________ _ ____ _______ _ ___ ____ _ nsa,lo3 ___ __ ________ _____ __ _____________ _ __ __ _ ~_ _ ___________ _ _ __ ~SBT lO~

____ __ _____________ ___ _____________________ ______ __________________ ___ ____ ~S~Tl05 IP~(??i __ ~ ________________ _ ______ ____________ ___ __ _ ________ ____ _______ ~!S~T106 lID122) lIDl~'!) llD(22) ?19. 5 ?18 . 3 ?18. 5 __ _ _ _ __ ~ ______ __.-______________ ______ __ __ ______ ______ _ __________ ~5~i~107 ~P!1(67) llDl3~
12t2. 4JI~ 2. ~i2. 8/3. 1 __ _ _____ ___ ____ _ ______ ___ ___ ____ _ __ ___ __ __ ____ _ __ __ ___ ___ ~!S~T106 2Ptl(ll) lP~(ll) q.sl7.e 15/6.5 _~___________________ __________________________ _ ________ _ _ _____ ___ . .
I~S~TIO9 ____________________ _ _____ __________ ______ _ __ _________ _ __ _ __ ___ _ _ ~58Tl lO lP~
10.31q. 1 _.________ _ __________ ____________ .________ ~S~TIll lPn(ll) ?13 _______________ ____________________ 115~T113 1Olq,5 ___~_ ______ I~S~Tll~ llgt33) 8ID13~3) 9 IID~33) WO ~2/13102 2 1 0 0 5 8 3 P(~lr/~ 92~0034~

TAB LE 5 ( C o n t i n u e d ~
NA~- HindlII Konl ~spI PstI Pv~II
= ========_=== ========= ======== ====== =========__=====_ ====~== ========= ======
~59Toqa ___ _____ __ __ ___ _ __ ________ __ _.__ ________ _ ____ _____ __ ___ ~S~TOqq 2ID(11) 2ID(11) 2ID(;l) 19121 2. 1 1~ . 9 ~. 9/~.
~PU(7~1 4P~(??) lOt9119 ?/4.2 _ _ _ ____ ___ ____ __ ___________ ___ ______ _________ _ _____ _ _ __ __ ___ ~59T100 IP~
~/11.5 ____________ ______ ___ _____ ___ _ _ ______ ___ ______________ ___ ___ ________ nSBT10; ??(??1 3P~(55~
3. 55~3. b ________ _ _______ _ __ ___ ________ __ __________________ __ _ __ ____ ___ ~S2T103 _________ _ ___ ___ __ ___.____ __ _ __ _____ ____ _______ _ _ _ ___ ~SBT104 _ ______ _ _______ ___ __ _.______ ___ _ _____ ______ __ _____ __ ____ __ ________ ~S~TIOS 2P~(9?) _ _ __ __ ~ __ ______ __________ ______ ________ ____ _ ____________ ___ ______ _ ~52T10~ 110122) ?13.7 __ _______ _________ _ _ _ ___ __ ___ __ _ ___ _ _ __ ~S~T10? 3?? (??) _ ______ _____________ ______________________ _ __________ ____________~_______________ ~SDT10~

_ ___ ___ __ _ _ ______ _ _______ _____ __ ______ ___ _______ _ __ __ _ _____ ~SBTIOq IID(55) lI0~55 ~,q~5.8/10.~ 3~
___ _ _ _ _ _ _______ _____________ _ _____ ___ _ ___ _____ ___ ____ _____ nSaTI10 ___ _ ________ _ _____.. _ _ _ _____ _____ _ ___ __________ _____ ____ ~S~T111 21D122) 3ID(llI
.ql3.2~2.a 7.519.2 __ ___ __ ______ _ __ _________ nS8T113 2P~1331 31Dlll) 7.5/3,4t4,2 ?110 3tD(ll) ~ID~55) ~/7-3.e 12~21/35 ________ ____ ~S~Tll~ IID133) lID133) lID133) llDt331 ?12.5 11/10 5.31S.9~1.b ?/~

~10~3 ~VO 92/13102 P~/l~lS92/OU340 TAB L E 5 ( Cont inued ) N~Y Taql Xbal HET.
================2=_================_==_===_===_ ' 1~53T09B ~P!~ ~11 ) 2.512.8 ___~__ _________________ ~________________ ~
~IS3T099 21D~11) 78 .91~. 8 ~ISBTOq~ 5Ptl~22) O
2.5/1.5 _________________ ___ ________ ___ ~l53T100 11 _____________ _________ _ ____ _ _ _ i~S~ lC~ 4PI~3) 1815/7.3 tlS8T101 5P~1~33) 13151? . 3 _ _ ______________________ ___________________ ~SBTl03 ______ ~_________ _______ ______ ___ ___ llSBT10~ 0 _ ________ ____ _ _ ____ _ _ _ _ ___ r.SBT105 Q

__________ ~____________________________ 1!59TlGb 22 _____________________________________ ________ r.SBT107 l!D(33) 78 2. 1/2.~
_________________________ ____________ _______ I~SBTlOE 3P~(50) 55 7/11.5 __ _____________________ ______________ _____ ~lS8T109 2P~ ~??) 55 _______ ____ __________________________ I'ISBTllO 2PH~??)~
_____ _____ ~ISBTlll 3ID~11) 22 7l8.8 ~SBTlll ~PI~ll) O
3.7l~.9 _______ IISBT113 3IDtll) t6 ?17 ~S8Tll~ ~I9~55) t6 .5t~.9 ~SBTll~ lID~33~55 ?1~. 9 WO 92/1~102 ~ O a S 3 P~r/l 'S92/00340 TA:E~LE 5 (Continued) N~t!' Ba2~I ~glI SglII EcsRI EooRV
=== = =.,===== ============ _ = ===== = = = ==== ======= == ==== _== === ====== ===_= .
.S3T11~ 2P~1 ~22) ?13. 5 _ ___ __ _____ ___ __~____ _ _ _ ___ __ __ __ ___ _ ____ __ ___ _______ ~sal~l6 _____ ____ ____ _ __ __ __________ _____ _ _ ____ _ ____ __ _____ _ ___ ____ _.. _ ~!S~T l 19 _____ _____ _ __ __ ___ _________________ ________ ___ ____ _____ ___ __________ I~S~Tl20 lP~
?17 _.. ___ __ ___ ___ _ ___ _________ ___ _ _______ _ ___ ___ _ ___ ___________ __ !ISBTl2, lPn~3) 2PY~33) sts.e/lo.s ~15 $
___ _________________ _ ____ __ _____ _ _______ _ _ ___ ____ ______ _ __ ____ ~VO 92/13102 ~ ~` 2 1 ~ O ~ ~ 3 PCr/US92/00340 TADLE 5 (Continued~ j Nar,~ HindlII Kpnl rspI Ps'il PvuII
====_====_=~==================S============_=====_=====i.=_============_===============_ ~
i~S8T114 ~Pri~22) 4P~1~55) ~3. 7/1 C. E 5/4 . 7l7 . 2 5P~5) Sl4 . 7l7 . 2 ~3g~Tll~ ??~??~ lPr.(~) 6.5l5.5 _ ~53~3T~I~ lI3D(~5) 11/5.2 ~!533Tl2C 2P~t443 7. 5/~. 1 _ _ _ _ ~IS~T121 WO 92/13102 2 1 0 0 ~ 8 3 PCr/~'S92/00340 - 8 ~--TA~ L E 5 ( Cont inued ) NA!~- T2qI XbaI H'T.
_ _ _ _ = = _, =--------_-------- -- ---- , I~SBT114 tlS~Tl 1~ Q
____________ ~____ _________________ llS8Tllh 33 _____ _______________ ____________________ __ llSBT119 llD~55) 67 4.212.B
I~SaTl~ 2P11~4~1 0 ~l7. 7 _ _ _ ~ _____________________ _ _________ ~!58T120 3PH(U~ 55 5,213.7 __ _ _ ________ ____ _ _ _ ________ _ ___ tlS8~1~; 3P~44) 6 3.415.2 __ __________= __________ _ __~________ _ ~092tl3102 2 l ao ~ ~ ~ PCT/US92/00340 Although this way of classifying RFLPs gives a conservative estimate of the num~er of identified polymorphi~ms, for cosmids characterized by strong linkage disequilibrium, the number of ID e~ents may be inflated at the expense of the actual number of PMs. To compensate for this, we performed a second set of calculations in which a polymorphic event must be detected with at least 3 enzymes to qualify as ID.
RFLPs previously attributed to insertion-deletion events because detected with two enzymes, are now considered as two independent point mutations. 24 polymorphisms initially consider~d IDs fell into this category.
Following this approach, 239 independent RFLPs were identified or 2.9 per cosmid, with now 87.9% of t:he PM
type and 12.1% of the ID type.
Table 5 reports the observed heterozygosities obtained with the generated multisite haplotype systems.
These values correspond to the percentage individuals heterozygous for at least one of ~he polymorphisms identified with a given cosmid. Noteworthy, this parameter is not affected by the mode of classification of RFLPs in PMs or IDs. At this point and without segregation information, we can't dissect the heterozygous genotypes into their compo~ent haplotypes.
2S These heterozygosities were estimated on a small sample and should therefore be considered cautiously. As pointed out by Skolnick and White (129), the main advantage of working with a sample of 9 individuals is that it is sufficient to identify the majority of useful pol~morphisms. However, the mean heterozygosity of 51.9%, obtained over the 84 polymorphic cosmids demonstrates the power of the approac~.
The following numbers of RFLPs were detected by each enzyme, irrespective of PM or ID type: TaqI: 57, EcoRI: 37, MspI: 33, HindIII: 31, PvuII: 30, EcoRV: 29, BamHI: 26, BglII: 24, XbaI: 21, PstI: 18, KpnI: 16 and BglI: 13. The number of polymorphisms detected with WO92/13102 2 1 Q ~ S 8 ~ PCT/~'S92/00340 BglII and PstI were corracted to adjust for the lower number of probes used with these enzymes.
Proper interpretation of the polymorphic patterns has been confirmed by segregation analysis in pedigree material for most of the described RFLPs (see hereafter).
RFLPs of the PM type were used to calculate nucleotide diversiti~s as described in Materials and Methods. Two sets of values are reported, depending on which of the two critPria were used to classify an RFLP
into the PM or ID type. Global nucleotide diversities of respectively 0.000652 and 0. 000846 were obtained, meaning that a randomly selected Holstein animal will be heterozygous for approximately l every 1200 to 1500 basa pairs. As expected because of the presence of an hypermutable cytosine followed by guanine in their recognition sequence, nucleotide diversities more than twice as high are obtained when combining data obtained with the enzymes ~spI and TaqI: ~.001493 and 0.002239 respectively (5). On the other hand, the recognition sequence of the enzymes BglII, HindIII, PstI, PvuII and XbaI are devoid of hypermutable cytosines in the CpG
dinucleotide and yield combined nucleotide diversities less than one third the values found with MspI and TaqI
(0.000492 and 0.000648). Using these two sets of values, one can extrapolate what nucleotide diversities would be obtained if sampling hypothetical sequences composed entirely of hypermutable cytosines, giving respectively 0.004496 and 0.007012. Assuming that the majority of detected polymorphisms behave according to the neutral mutation-random drift hypothesis, nucleotide diversity and mutation rate are simply related as:
~ ~ 4Ne~, where Ne stands for the effective population size and ~
for the mutation rate (l). Therefore, our data allow us to estimate that cytosines followed by guanines mutate at a rate approximately l0 times higher compared to ~092/13102 ~1 0 0 5 ~ 3 PCT!US92/00340 other nucleotides, presumably because a substantial fraction of these are methylated in the germline and prone to mutate to thy~idine by spontaneous deamination.

D~8~8I0~

We demonstrate in this work that large numbers of DNA markers with very acceptable Polymorphism Information Content can be quickly generated using large, randomly selected genomic probes in Southern blot hybridization experiments. The multisite haplotypes identif ied in this study using cosmid prsbes have a mean heterozygosity of 51.9% This value is of the same order of ma~nitude as the heterozygosities that we have obtain~d with a panel of approximately 4 0 bovine Variable Number of Tandem Repeat markers (mean heterozygosity 59~; 150), and with more than 50 bovine (TG3-dinucleotide microsatellites (mean heterozygosity 56%; unpublished).
A remarkably high proportion of the tested cosmids proved informati~e: 74.5% of all tested clones, and as - ~0 high as 85% when considering only the clones giving readable patterns~ Compared to strategies aimed at isolating hypermutable seguences such as mini- or microsatellites, very little time and effort is wasted into candidate clones which have to be dropped at a later stage.
Because the cosmid clones used as probes are selected at random, we can reasonably assume that the coverage obtained with the generated markers is fairly uniform. Monte-Carlo simulations allow us to estimate that these 82 markers are covering 29%, 47% or 60% of the bovine genome in linkage studies if a maximum of respectively 5, 10 and 15 cM are scanned on each side of each marker, and assuming a total bovine map length of 25 Morgan as deduced from chiasmata counts (151).

WO92~13102 ~ 1 ~ O ~ 8 3 PCT/~'S92/00340 The Southern blot hybridization procedure used for the detection of these RFL~s is a very mature and robust methodology, allowing the treatment of very large numbers of samples simultaneously, and benefitting from intrinsic '~multiplexing" properties especially when using nylon membranes. Indeed, and despite variations between batches, we are routinely using membranes for lO
or more hybridization cycles.
The main disadvantage of multisite haplotypes is the requirement to use several restriction enz~nes to fully exploit their PIC. This increases costs, amounts of required DNA, complicatas the organization of genotype collection and their subse~uent use in linkage analysis.
lS The fact that 75 to 85% of ~he cosmids tested in cattla reveal polymorphism, compàres favorably with results previously reported in the human. Schumm et al.
~131) for instance report that 30% of the 1664 lambda clones they tested in a sample of 5 individuals, gave polymorphic patterns. Adjusting for the sample size and a ratio of approximately 2.5 between cocmid and phage insert size, the two figures are probably fairly similar. Surprisingly, only 54 of 101 human cosmid clones tested by the same group (152) were revealing RFLPs when tested with 9 restriction enzymes, versus 74.5~ in our study with, however, 12 enzymes. It has been speculated that the relatively low level of polymorphism found in this study might result from a biais against human methylated sequences (including CpG
present in the recognition sequence of TaqI and MspI ) when construction the cosmid library, due to the active modified cytosine restriction system (mcr) of the E.Coli 1046 host (152).
These results are quite unexpected. Indeed, becau~e of the population structure imposed by breeding strategieC~ the effective population size, Ne, in cattle is sxpected to be significantly lower than in the human.

~092/l3102 2 1 ~ o ~ 8 PCT/ ~S92/00340 In American Holstein, Ne is estimated between lo2 and 2x103 (Ina Hoe~chele, personal communication). This value has to be compared with an estimated Ne of 104 in the human (18~. Assuming identical mutation rates, this reduction of Ne should be accompanied by a concomitant reduction in genetic variation. As a matter of fact, we obtain estimates of global nucleotide diversity between 0.000652 and 0 000846 which are between 3.5 and ~.5 times lower than value~ typically found in human populations (2, 119, 132). This confirms our previous results in another cattla population: the Belgian Blue Cattle br~ed t3, 4).
At least part of the discrepancy between an apparently reduced nucleotide diversity but similar RFLP
frequency, may be accounted for by the apparently higher fre~uency of insertion-deletion events found in cattle compared to human. Schumm et al. (131) report that 58 out of the 515 polymorphic loci (11.26%), show insertion-deletion type RFLPs; other groups report even lower frequencies of such events in the human (R.White, personal communication~.. In cattle, we found that 29 (35%) to 46 (56%) out of 82 polymorphic cosmids show ~uch insertion-deletion events, depending on whether an ID-type polymorphism has to be detected with two or more enzymes. These results seems to point towards a fundamentally different property of both genomes~ It is tempting to speculate that this high level of insertion-deletions in the bovine genome reflect the activity of a mobile element. Analysis of the restriction patterns charactarizing these ID events, however, does not reveal any typical, recurrent "signature" of such an element.
Altogether, our laboratory has now isolated more than ~00 DNA markers for cattle with a mean heterozygosity above 50%: 82 multisite haplotypes, 40 Variable Number of Tandem Repeat mar~ers (150) and more than 80 dinucleotide microsatellites (unpublished). The coverage of the bovine genome obtained with increasing WO92/13102 210 0 5 8 3 PCT/US92/00340 ;

number of randomly selected prob~s (o to 500)t was estimated by Monte-Carlo simulation assuming that the used family material is sufficent to detect linkage at re pectively 5, lO and 15 cM, andg a total bovine map length of 25 Morgan as deduced from chiasmata counts (151), divided over the 30 bo~ine chromosomes according to their relative length. With the PIC characterizing our marker set, we feel fairly comfortable that in the majority of situations we will be able to cover genetic distances of the order of lO cM or more, especially if applying multilocus or interval mapping techniques (66, 68). Therefore our panel of probes should cover around 75% of the bovine genome.
It is obvious that we are approaching a point: where the efficiency of a strategy based on the further accumulation of random markers become questionable.
After 200 probes, the additional coverage obtained per new marker, expressed as a fraction of the maximum coverage possible, now is approximately l/5 of the coverage obtained when we started this project. This creates the need for more targeted approaches. In this regard, mappers of domestic animal genomes will benefit from the human mapping efforts and the remarkable chromosomal conservation observed within mammals (133).
Based on comparative mapping information, it should be possible to identify genes likely located in the "holes"
left by the random approach and to generate multisite haplotypes or microsatellite markers around their bovine homologues. ~e are presently exploring the feasibility of such approaches.
With the markers available today, a substantial part of the bovine genome is now amenable to linkage scanning, which will hopefully allow the mapping of Economic Trait Loci and testing the feasibility of Marker Assisted Selection schemes.

~0 g2/13102 2 1 0 0 ~ ~ 3 PC~/USg2/00341) CLONING, CAR~CT~RI2ATION AN~ "~N VITRO" AM~LIFTC~TION
OF BOVIN~ ~IC~08AT~h~T~S

I~TROD~CTION
Recently, microsatellites were proven to be an abundant source of highly polymorphic markers in the human (32-34~. As their name implies, microsatellites are minute VNTR markers (18-20), charactPrized by tandem repetitions of very short repeats, one to four base pairs in length. Microsatellites exhibit levels of polymorphism comparable to VNTRs, but are much more abundant and apparently evenly spread throughout the genome.
We describe the cloning, caracterization and "in vitro" amplification of more than 100 such bovine microsatellites~

MATERIA~ AND NET~OD~

1. DNA Datab~e 8e~rch Bovine and ovine sequences in the EMBL and &enbank (v~rsion 64.0) were searched for all types o~ dinucleo-tide and trinucleotide repeats using the Intelligenetics software, release 5.37. The minimum number of repeats was set at six. Six bovine sequences, characterized by the longest microsatellites, were retained ~or further analysis and are listed in Table 6.

GBFSH GBXCAS
GBGAPR GBPRLGR

WO9~/~310. 21~ O a ~ ~ PCT/U~92J00340 2. I~ol~tio~ of Bo~in~ ~icros~tell~t~s Bovine genomic DNA was digested to completion with MboI and size-fractionated by agarose-gel electrophore-sis. Fragments between 250 and 500 b~se p~irs were recovered and purified using l'Gene-Clean", ligated into the BAP-dephosphorylated ~mHI site of pUC13 (Pharma-cia), and cloned into E. Coli D~5~ cells (BRL). The resulting lones were screened for the presence of ~TG~n microsatellites using a 32p kinased (AC)l5 oligonucleotide as probe, and for (AG)n microsatellites using a ~TC)15 probe. The library was made with female DNA to avoid the previously characterized Y-specific TG-rich bovine DYZl sequence (117).

3O 8eque~ci~g of Bovi~ ~icro~t~llit~s Positive clones were sequenced using one of the following procedures:
(a) Plasmid DNA was prepared using standard "boil-i~g mini-prep" procedures and subjected to two chain-termination sequencing reactions using unmodified T7 DNA
Polymerase (Pharmacia), with the "universal" and "re-verse" sequencing primers, respectively. The 35S labeled sequencing products were analyzed on standard denaturing polyacrylamide sequencing gels and detected by auto-radiography.
(b) Maaneti~ solid-phase seouencinq ~137). Alterna-tively, positive colonies were grown in microtiter-format using standard procedures in order to establish ~lycerol stocks and 5~1 of culture directly subjected to PCR amplifications using the following vector-specific primers:

UNIBIS: 5'-GATGTGCTGCAAGGCGATTAAGTTG-3' REVBIS: 5'-CGGCTCGTATGTTGTGTGGAATTGT-3' Two 30 cycle amplifications were carried out per clone, one with the UNIBIS primer biotinylated, the other with ~O9~/13102 ~ 1 0 ~ ~ 8 3 PCT/US92/0034~

the REV~IS primer biotinylated. Denaturation was at 93 C
for l min. (except for the first cycle: 95 C for 5 min.) annealing at 60 C for 2 min., and extension at 72 C for 2 min. All of the PCR reactions were performed in micro-titer-format using the TECHNE MW-2 heating device. The biotinilated strand of the PCR product was captured using the DYNAL streptavidin-coated magnetic beads according to the manufacturer recommended ronditions and sequenced using unmodified T7 DNA polymerase (Pharmacia) as specified by the manufacturer.

4. Amplification and D~tection of Bo~i~e ~icro~at~llit~
(a) Simlex Amplification. The generated sequences are organized in the following way:

5'-.... (UP).....(TG)n.... .o.... ,.~3t 3'-............. (AC)n.... (DN)...... 5 Suitable primers for n vitr~ amplification are identi-fied in "UPSTREAM (UP)" and "DOWNSTREAM (DN)" strands using the "OPTIPRIM" pro~ram designed by one of us.
Given sequ2nce information flanking a DNA strPtch, "Optiprim" helps the user to identify suitable primer pairs for PCR amplification of the corresponding DNA
stretch.
DescriptiQn of tb~ pro~am: The two DNA sequences flanking the DNA stretch to be amplified are referred to as the upstream (UP) and downstream (DN) sequence, res-pectivGly. Both for UP and DN, Optiprim tests all possible primers of given length (as defined by the user) and retains the primers (l) with melting tempera-ture (Tm) within the range defined by the user (Tm is calculated as 2C x number of As, or Ts + 4C x number of Gs or Cs), (2) with a minimum percentage of each nucleo-tide as defined by the user, and (3) which cannot form secondary bonds that can be formed between two molecules W092t13102 21~ 0 3~ 3 PCT/US92/00340 of the defined primer when sliding them in antiparallel orientation against each other, as illustrated in the following:
5'-PRIMER-3' --->
<--- 3'-REMIRP-5' An A facing a T contribute~ two hydrogen bonds, and a ~ facing a C contribu~es three hydrogen bonds. No loop formation is considered when performing this analy~is.
This generates two sets of selected primers: an UP set and a DN set. All po~sible pairs of one UP and one Dn primer are then tested. Optiprim retains the primer pairs if (l) the difference between melting temperatures of the two primers is within a range defined by the ussr, (2) the two primers cannot form secondary struc-tures, determined as for individual primers, except that now the UP primer is slided versus the DN primer. Using this program, 80% of the selected primer pairs were giving successful PCR amplification in our microsatel-lite systems. The following criteria are considered by 'tOPTIPRIM's when searching for primers: primer length, meltin~ temperature and secondary structures that can be formed within and between primers. The selected primers are synthesized by phosphoramidite chemistry on Applied Biosystem synthesizers and used without further puri-fication. The microsatellites are amplified n vitro, in microtiter plates and using the Techne MW2 device, in the following conditions (typically, 30~1 reactions):
Target DNA 50 ng-lOOng KCl SOmM
Tris-HCl, pH 8.4 lOmM
MgCl2 l.5mM
Gelatine 0.01%
dNTP 200~M each Primers l~M each dCTP32 2~Ci/30~1 Amplitaq lU/30~l WO g2~13]02 2 1 Q ~ ~ ~ 3 PCT/US92/00340 Thirty cycle amplifications are performed, characterized by a 93 C denaturation for 1 min. (except for the first cycle: 95 C, 5 min.), annealing at 55 C, 60 c or 65 C for 2 min. depending on the primers, and extension at 72-C
for 2 min. Annealing temperatures are reduced by 5-C
when using bovine primers on ovine target DNA.
(b~ Mult~lex Amplification. When performing multi-plex amplifications, concentrations of KCL, Tris-HCl, MgC12, gelatine and dNTPs are increased by 50~, while the primer concentrations are decreased to 160~M each~
(c) Detection of Microsatellite_Products. 2~1 of PCR reaction are mixed with the same volume of for~amide dye and run in a denaturing 7% acrylamide, 32% forma-mide, S.6 M urea, 13.5 mM Tris, 4.5 mM Boric Acid, 250 ~M EDTA gel. 32p labeled products are detected by auto~
radiography.

R~R~I.T8 A~D DT8CIJ88ION

1. Clon~ng ~ gu~n~ c~ract-ri3~tio~ of bo~ine mi¢rosat~llite~:
A library of MboI fragments between 250 and 500 bp was screened with the oligonucleotide probes. One out of 50 clones cross-hybridized. Assuming independent distri~ution of microsatellites and MboI sites, the frequency of (TG)~29 microsatellites in the bovine genome is estimated to be at >100,000. Table 7 summarizes the sequence information available for about 230 such bovine microsatellites. For each of these, sufficient sequence information has been gathered to generate the required primers for PCR amplification of the corresponding microsatellite. All sequences were generated by sequencin~ as described above or by screening GENEBANK.

WO92/13102 210 0 ~ 8 3 PCT/~'S92~00340 o98--Bovine Microsatellites Sequence Sequence Identificatio~ N ~ ers Name Up Repeat Down _________.______________________________._________ AGL~232 43 44 45 AGhA291 100 101 102 AGhA293 103 104 105 AGhA296 106 107 108 W 0 92/13102 ~ 1 0 ~ ~ 8 3 PCT/~IS92/0034fl _g9_ TABLE 7 (Continued) MGTGl 136 137 138 MGTGll 139 140 141 MGTG13B 145 146 14, M&TG3 148 lag 150 ~GTG7 154 155 156 TGLA109 163 16~ 165 TGLAllO 166 167 168 TGLAlll 169 170 171 TGLAl42 229 230 231 !

W O 92/13~0~ 2 1 0 0 5 8 3 PC~ Sg~/00340 TABLE ? (CGntinued) TGLÆ175 274275 276 TGLA17g 280281 282 W 0 92/131~2 2 1 0 ~ ~ ~ 3 PCT/~;S92/00340 ~ABLE 7 (Continued) WO 92/13102 2 1 0 0 ~ 8 :3 PCr/~lSg2/00340 TABLE 7 (Continued~

TGLA34~ 454 455 456 21~0~3 ~VO 92/1310~ PCr/l,'S92/00~40 TABLE 7 (Continued) 02 PCr/US92/00340 TABLE 7 ( Continued) TÇLA80 664 665 666 T&LA86 676 677 678 WO92J1310~ 3 PCT/~1S92/00340 2. PCR ~plificat~on ~d D~te~tio~ of Xicrosata~lit~Y
(a) Slm~lex am~lification Table 8 reports a preliminary list of bovine micro- '~
~atellite systems that were s~cces~fully amplified ln vitro, with the corresponding primer pairs. Note that pairs of primers selected by "OPTIPRIM", allow success-ful amplification in at least one of our standard con-ditions more than 80% of the time. Table 9 also gives the favoured annealing temperature (using the TECHNE MW2 heating device). The mean heterozygosity for the bovine microsatellites was estimated at ~50~.

WO92tl3102 213 0 ~ 8 3 PCT/~IS9~/00340 PCR Amplified Bovine Microsatellites Up Up Down Down Sequence Primer Primer Primer Primer Name Name ID Name ID Annealing __________________________________________.______________________________ AGLA13 AGLA13U~1 697 AGLA13DN1 698 55 60 AGLA209 AGLA209U~1 701 AGLA209DN1 702 55 6C
AGLA215 AGLA215UP1 703 AGLA215DN1 70~ 60 AG~A217 AGLA217UP1 705 AGLA217DN1 706 55 60 AGLA22 AGLA22UP1 707 AGLA22DNl 708 60 AGLA254 AGLA254UP1 713 AGLA254DNl 71~ 'i5 6C

AGLA29 AGLA29UP1 727 AGLA2~DN1 728 55 60 GBFSH GBFSHUPl 735 GBFSHDNl 736 55 GBI~BP GBIRBPVPl 737 GBIRBPD~l 738 60 GBKCAS GBKCASUPl 739 G~KCASDN2 740 60 MGTGl MGTGlUP3 741 MGTGlDNl 742 55 60 MGTG13B MGTG13~UP3 743 MGTG13BDN2 744 55 60 TG~A10 TGLAlOUPl 751 TGLAlODNl 752 6C
TGLAlll TGLAlllUPl 753 TGLAlllDNl 754 60 TGLA116 TGLA116UP1 755 TGLAl16DN1 756 60 TGLA122 TGLA122UP1 761 TGLA122DNl 762 60 TGLA123 TGLA123UPl 763 TGLA123DN1 764 60 TGLA125 TGLAl25UP2 767 TGLA125DN2 768 55 60 TGLAl30 TGLA130UP1 775 TGLAl30DN1 776 55 21QQ~
~VO 92/13102 PCT/us92/0~340 T~BLE 8 (Continued) TGLA142 TGhA142UP1 783 TGLA142DN1 784 60 TGhA170 TGLA170UP1 797 TGLA170DN1 798 60 TGLA182 TGhA182UP1 801 TGLA182DN1 802 60 TGLA203 TGLA203UP1 803 TGLA203DN1 80~ 60 TGLA340 TGhA340UP1 853 TGLA340DN1 854 55 60 WO ~2/13102 2 1 ~ 0 5 ~ 3 PC~/~'S92/00340 TAB~LE_~ (Continued) TGLA357 TGLA357UPl S69TGLA357DN1 870 55 60 TGLA377 T5LA377UP1 875TGLA377DN~ 876 5; 60 TGLA415 TGL~415UP1 885TÇLA415DN1 886 55 60 TGLA441 TGLA441UPl 901TGLA441DN1 902 60 TGLA48 TGLA48UPl 909TGLA48DN1 910 55 60 TGLA49 TGLA49UPl 911TGLA49DN2 912 55 TGLASl TGLASlUPl 913TGLASlDNl 914 60 TGLA53 TGL~53UP1 917TGLA53DN1 918 55 60 TGLA58 TGLA58UPl 919TGLA58DN1 920 55 60 TGLA6QA TGLA60AUP1 923`TGLA60ADN1 924 55 ~O 92/1310~ 2 L 3 ~ 5 S ~ P~/l~'S92/00340 TABLE_ 8 ( Cont inued ) TGLA77 TGLA77UPl 941 TGLA77DN1 942 55 60 WO92/13102 PCT/US92!00340 (b) Multiplex Amplification To increase the speed and lower the cost of geno typing, multiplex approaches for both amplification and data capture of microsatellites are utilized. Micro-satellite systems yielding products of non-overlapping size were coamplified as described above. Preliminary results show that at least four different systems can easily be coamplified in these standard conditions. The following multiplex amplifications, for instanoe, were shown to yield consistent, easily interpretable results:

a . GBCYP2 1 - TGLAl O - TGLA 4 4 - TGLAl 16 b . TGLA9 - MGTG4 B - TGLA2 3 - TGI~3 5 c. MGTG3 - MGTG13B

By limiting detection to a single detection procedure lS (autoradiography of 32P-labeled product3, multiplex amplification is limited to systems yielding products of non-overlapping size. To overcome this limitation, alternative detection schemes are utilized. In particu-lar, the use of confocal microscopy to detect products labeled with laser-excitable fluorescent molecules (such as fluoresceine, rhodamine, ...) is used. The products can then be differentiated based on the specific excita-tion and emission spectra of the tagged fluorescent molecules. Using this approach detection of up to at least 20 different systems should is feasable.

3. PCR-mapping of Bo~ine Micro-satellites Using ~om~tic Cell ~ybrid~
Results of the concordancy analysis are summarized in Table 9. Synteny groups to which microsatellite systems most likely map as deduced from concordancy analysis are underlined. Clear-cut results were obtained for MGTGl3B (Ul9 or chromosome 15), TGLA6 (Ull), TGLA9 (U27), TGLAll (U16), TGLA22 (U26 or chromosome 26), TGLA23 (Ull), TGLA36 (U27), TGLA52 (U9 or chromosome ~092/1310' 2 ~ 3 0 ~ ~ 3 PCT/US92/00340 18). Results are less di~criminating for the other systems. Most likely synteny groups are, however, mentioned. In addition, we know from the literature ~, that GBKCAS maps to Ul5 or chromosome 6, and GBCYP2l to U20 or chromosome 23.

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Bovine pedigrees for a total of approximately 200 individuals were genotyped for 150 of these markers as described. Pair-wise linkage analysis was performed using the L0DSCORE program. Only lodscore values superior to 3 wer~ considered significant. This generated a primary DNA marker map with 24 linkage groups counting two or more markers (15 assigned to speci~ic chromosomes or synteny groups), and 68 singleton markers. Table 10 summarizes our findings.
Linkage groups were assigned to pecific chromosomes or synteny groups whenever that information was available.
TAB~E 10 Primary Bovine DNA Marker Map CHR.~SYNT. LG MARK~R
Chr.2 1 GMBT28 ! 1 TGLA58 2 Weaver Chr.6 GBKCAS
Chr.~ GMBT17 WO 92/13102 ~ 1 0 3 i ~3 ~ PCr/~lS92/00340 TABLEI0 ( Continued) CHR. /SYNT . LG MARKER
Chr~ 10 GMBTl9 Z
Chr. 14 Thyroglobulin Chr. 15 3 MGTG13B

4 TGL~75 Chr. l9 G~

Chr. ~1 5 GMBTl5 kSSBT2 9 TGI~3 3 7 Chr. 23 6 GMBT12 6 Prolactin 6 BoLA

~5 6 AGLA291 6 TGL~14 2 7 TGL~153 Chr. 24 8 GMBT5 WO92/13102 2 1 ~ O S ~ 3 PCT/US92/00340 `

TABLE 10 (Continued) CHR./SYNT. LG MARRER
Chr.26 GMBTll Chr.X GMBT27 Chr.Y DYZl Ul 9 GMBT42 Ull 12 T&LA6 U16 TGL~5 GM3Tl 13 TGLA206`
U~22 GMBT14 ? 15 GMBT3 10' ~ 8 3 PCT/~S92/00~0 ;117-TABLE 10 (Continued) CHR./SyNT. LG MARKER
? 16 GMBT21 ? 17 GMBT24 17 TG~A164 17 TGL~48 17 T~LA303 ? 18 GMBT18 ? 19 AGLA226 19 TGL~28 ? 20 MGTG1 ? 21 M5BTll 21 MSBTl9 ? 22 TGLA378 ? 23 TGLA51 23 TGI~94 ? 24 TGhA54 2S 24 T&LA68 CHR./SYNT.: Chromosome or synteny group LG: Linkage group CON~LU~IONS
Samples of E. coli harboring clones of polymorphic bovid markers have been deposited on 17 January 1991 with the American Type Culture Collection (Rockville, Maryland) under accession numbers 68,514 and 68,515~
35Deposit of the clones i5 for the sake of completeness, but is not intended to limit the scope of the instant invention to said deposited materials. Access to said WO92/1310~ 2 1 ~ O S 8 3 PCT/~ISg2/00340 cultures will be available during the pendency of the application to those determined by the Commissioner of Patents and Trademarks to be entitled ~hereto. All restrictions on availability will be removed upon grant of the application and said cultures will remain avail-able during the life of the patent. Nonviable or destroyed cultures will be replaced in kind.
It will be appreciated that the methods and com-positions of the instant invention can be incorporated in the form of a variety of embodiments, only a few of which are disclosed herein. It will be apparent to the artisan that other embodiments exist and do not depart from the spirit of the invention. Thus the described embodiments are illustrative and should not be construed lS as restrictive.

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~21. JARMAN, _ et al. (19 ) _ _ 122. BELL, et al. (19 _ 3 123. COPP~ETERS, W., et al. (19 ). ~nim,~LI~ _etics 21:
29-30.
2S 124. JEFF~EYS, A.J., et al. (lS88). Nature 332:278-280.
125. JEFFREYS, A.J., et al. (1988). Na~ure ~ 278-~80.
126. HITT~T et al. (1989). An'm. Genetics 20, 145-155.
127. FARBER, ~.M., et al. (1989). ~uman Genet. 182:1-3.
128. BISHOP, D.T., et al. (1983). Am. J. Human Genet.
33:795-81S.
129. SKOLNICK, M.H. and WHITE, R. (1982). CYtoqenet.
Cell Genet 32:58-67.
130. EWENS, W.J., et al. () 1981). Proc. Nat. Acad. Sci.
USA 78:3748-3750.
131. SCHUMM, J.W. et al. (1988). Am.J.Hum.Genetics 42:
1 143-159.
132. COOPER, D.~., et al. (1985). Human ~enetics 69:
201-205.
133. WOMACK, J.E. (1987). Dev~lo~e~t. Genet. 8:281-293.
134. RIORDAN, J.R., et al. (1989). Science 245:1066-1073.
~' 135. SHOWS, T.B., et al. (1983). Advances l n Human Genetics 12:341-452.

.

WO92/13102 2 1 0 0 5 8 3 PCT/~TSg2/00340 136. FALCONER, ~.S. (1989). IntroductiQn ~o ~antitative Genetics, 3rd Ed., Longman Scientific & Technical.
137. HULTMAN, T., et al. (1989). Nucl. Acids ~esearch 17~13~:4937-4946.
138. ORKIN, S.H. (1986)- ~11 47:845-139. QUESADA, M.A. et al. (1991). Biotechniques (in pre~s).
140. HOESCHELE, I. and MEINERT, T.R. (1990). J. Dairy Sci. 73 2503 _ .
141. GEORGES, M. and MASSEY, J.M. ~1991). ~e~ioaenoloqY
35:151.
~42. GEORGES, M. (1991). In Anim~l A~liQations of Research in Mammalian Development, Pedersen et al., Eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbour, New York, pp. 285-325.
143. GEORGES, M. et al. (1988). Nucl. Acids Res.15:7193.
144. FRIES, R. et al. (lg90). In Gene ~a~pinq:
Strateaies, Technigues and Applications, Schook, L.B~ et al., Eds., Dekker, New York.
145. JULIER, C. et al. (1990). Proc. ~at. Acad. Sci. USA
87:4585-4589.
146. WONG, Z. et al. tl986). Nucl. Acids ~es. 14:4605-4616.
147. WONG, Z. et al. (1987). Ann. Hu~. Genet. 51:269-28~.
148. STEET~, M.R. and GEORGES, M. (19gl). Ge~ ics 10:889-g04.
149. FRIES, R. et al. (1990). Genomics 8:403-406.
150. GEORGES, M. et al. tl991). Geno~ics (in press).
151. LOGUE, D.N. and HARVEY, M.J.A. (1978). ~. Re~ro.
Fertil. 54:159-165.
152. 80WDEN, D.W. et al. (1989). J.. Human. Genet.
44:671-678.

rl -` 2LOO~j~3 ~092/1310~ PCT/~IS92/00~0 SEQUENCE LISTING

(1) GENERAL INFORMATION:
ti) APPLICANT: Georges, Michel Massey, Joseph M
( ii ) TITLE OF IN~ENTION: POLYMORPHICDNA MAR~OERS IN
BOVII:~AE
NUMBER OF SEQUENCES: 952 (iv~ CO~RESPONDEN OE ADDRESS:
(A) ADDRESSEE: Venable, Baetjer, Howard &
Civiletti (B) STREET: 1201 New York Avenue N.W., Suite (C) CITY: Washington (D) STATE: DC
~E) COUNTRY: USA
(F) ZIP: 22205 (Y) COMPUTE~ READABT~ FORM:
(A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS
(D) SOFTWARE: Wordperfect 5~1 (vi) CURRENT APPLICATION DATA:
tA) APPLICATION NUNBER: WO
(B) FILING DATE: 15-JAN-1992 (C) CLASSIFICATION:
(vii) CURRENT APPLICATION DATA:
(A) APPLICATION NU~BER: US 642,342 (B) FILING DATE: 15-JAN-l99l (C) CLASSIFICATION:
(viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: Ihnen, Jeffrey L
(B) REGIST~ATION NUMBER: 28,957 (C) ~EFERENCE/DOCXET NUMBER: 18681-91367 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: 202-962-4810 (B~ TELEFAX: 202-962-8300 W O 92/131~2 2 1 0 0 S 8 ~ PCT/~ISg2/00340 `

~2) INFORMATION FOR SEQ ID NO: l:
(i) SEQUENCE CHARACT~RISTICS:
~A) LENGTH: 45 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(Yi ) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLAl3 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: l:
GGCGAGCTCGAATTCTTACTCTTCTGTTTCCTTTGTTTAAATCAA
(2) INFORMATION FOR SEQ ID NO: 2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 79 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLAl3 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2:
TATCTATCTATCTGTGTATATATGTGTGTGTGTATATATATATATATATA
AAGAGAGAGAGAGAGAGAGAGAGAGAGAG
~2) INFORMATION FOR SEQ ID NO: 3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 50 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linea_ (ii) MOLECULE TYPE: DNA (ge~omlc) 0 J - ~ C '` ' ~' L . . ~ . v ~O 92/13102 2 1 ~ ~.3 ~ 3 PCT/~TS92/0034~

-~29-(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IM~EDIATE SOURCE:
(B) CLONE: AGLAl3 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3:
AACCAGCACCATGGAAA TCTCAGTCCACTGAGTAGTACTGATAAAATG~
(2) INFORMATION FOR SEQ ID NO: 4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 142 base pairs (B) TYPE: nucle~c acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HVPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLAl7 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:
TGCTCCCTGTTAGCAATGGGATGTGAAAAGCTGAAAAAAGGTACAGAGAT
GCTCTGTCCAGTATGAGAGCCACATATGACTGTAAAGCCCCTGAAATAGA
CCTAGTCTGAACCGAGACGCACACAAGGATGTGAACTGTTGT
(2) INFORMATION FOR SEQ ID NO: 5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHE~ICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLAl7 ~repeat) (xi) S~QUENCE DESCRIP~ION: SEQ ID NO
AAA~AGAAAAAAAGAGAGAGAGAGAGAGAuA.

WO 92/13102 2 1 0 0 ~ 8 3 PCI`/US92/00340 "

--l 3l~--(2) INFORMATION FOR SEQ I~ NO: 6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 189 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) Y.YPOTHETICAE: NO
(iv) A~TI-SENSE: NO
(~i) ORIGINAL SOURCE:
~A) ORGANISM: Bos tau-us (Vii) IMMEDIATE SOURCE:
(B~ CLONE: AGLA17 (down) (Xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:
TTCCCAACCCAGGGATCAAACCGAGGTCTCCTGCACTGCAGCTGATTCCT
TACCATCTGAGCCACGAGGGTAACCTAATGTGGGCCAAACAGGTAGTTTA
AAATTTTCTAGTAACCATGTTAAATAAGTAATTCAATCAATTATAATATA
TTTTCTTTAACCTAATAATCCAATGTATTATCATCT...
(2 ) INFORM~TION FOR SEQ ID NO: 7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: ~l base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ( genomic) (iii) HYPOTHETICAL: NO
(iV) ANTI-SENSE: NO
(Vi) ORIGINAL SOURCE:
(A) ORGANISM: BOS taurus (~ii) IMMEDIATE SOURCE:
(B) CLONE: AGLA20 6 (up) (Xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7:
TTACAATATATCAAAGTATCTGTTGTAATATTTAGACTCATAAGATGGAA
CACTACAGACC
(2~ INFORMATION FOR SEQ ID NO: 8:
(i) SEQUENCE C~ARACTERISTICS:
(A) LENGTH: 14 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linea (ii) MJ~ECU;_ TYP_: DN~ (geno.

~0 92/13102 2 1 0 0 ~ 8 ~ PCT/US92/~0340 -l3l i (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) C~ONE: AGLA206 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:
~A.~A~
(2) INFORMATION FOR S~Q ID NO: 9:
(i) SEQUENCE CH~RACTERISTICS:
~A) LENGTH: 42 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
IB) CLONE: AGLA206 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9:
AAAGACTCAGAAAGAAGGCTGTGTCCCAAGGGCAATGAGTGA
(2) INFORMATION FOR SEQ ID NO: l0:
(i) SEQUENCE CHA~ACTERISTICS:
(A) LENGTH: 79 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA209 (up) ~xi) SEQUENCE DESCRIPTIO~: SE~ }D NO: I0:

W O 92/~3102 2 1 0 0 S 8 ~ PCT/US92/0034~ ~

'~ 3 2 -GGGAGTAAGCCAGACAGCCTGATGGAGACAGAGTCAGAGACAAAGAGACC
TGGCTGCCAAATTCCCCCTCATCTCCTAC
(2) INFORMATION FOR SEQ ID NO~
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii~ HYPOTH~TICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA20 9 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:
AGAGAGAGAGAGAGAGAGAGAGA
~2) INFORMATION FOR SEQ ID NO: 12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs B~ TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY:. linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SE~SE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (v~i) IMMEDIATE SOURCE:
(B) CLONE: AGLA209 ( down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:
AGCTGCTTCTGCCCATCCCTTTATT
(2) INFORMATION FOR SEQ ID NO: 13:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 43 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECUL- TY_: DNA ~ genomi_) ~0 92/13102 21 Q ~ 3 8 3 PCT/US92/00340 ~~3 3-(iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE: 7 (A) ORGANISM: Bos taurus (Yii) IMMEDIATE SOURCE:
(B) CLONE: AGLA212 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:
GTGATTTTCTTGTTTAAACAAT~CCTGC~TAAAGACGCCACT~
~2) INFORMATION FOR SEQ ID NO: 14:
(i) SEQUENCE CHARACTE~ISTICS:
(A) ~ENGTH: 61 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE. DNA (genomlc) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos tau~us ~v~i) IMMEDIATE SOURCE:
(B) CLONE: AGLA212 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:
AaAAAAAAAAAATCCAGACATAGAAACCACAGAGAGAGAGAGAGAGAGAG
AGAGAGAGAGA
(2) INFORMATION FOR SEQ ID NO: 15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 73 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ( Yi i ) IMMEDIATE SOURCE:
(B) CLONE: AGLA212 (down) (xi) SEQUENCE DESCRIPTIO~: SEQ ID NO: 15:
GG-GG-TCTGT-TT.AG-TTC~AAA-TATTAT~A~AAA--_AT~ G-W O 92il3102 210 0 ~ ~ 3 PCT/US92/00340 `

TTATTTTTAGGGTCATCCTTCCT
t2) INFORMATION FOR SEQ ID NO: 16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 96 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii~ MOLECULE TYPE: DNA (~enomic) (lii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA215 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:
CACAGGTTGCATGCTTAGGATAAGGACCAGGCCTGAATGTCCTGAGGCCA
GTCTGAGAGACTAACATGAGATAGCAACCCAAACTGTGAGACAGCC
~2) INFORMATION FOR SEQ ID NO: 17 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 45 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii~ IMMEDIATE SOURCE:
(B) CLONE: AGLA215 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:
AGAGAGAGAGAG~A~A~AAA A~AAA GAGAGAGAGAGAGAGAGA
(2) INFORMATION FOR SEQ ID NO: 18:

(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOL~CULE TYPE: DNA (geno~.ic~
(iii) HYPOTH-TICAL: N~

.

~O g~/1310~ 2 l a o ~ ~ ~ PCT/~S92/00340 ~~3 5-~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA215 ~down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18:
AGCTTTTCGTGGGAAAGT
(2) INFORMATION FOR SEQ ID NO: 19:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 62 base palrs ~B ) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA217 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:
AGTGGTTGCCATTTCCTTC~CTAATGCATGAAATGTGAAAACCACTAGCC
AGACTCATAAAG
(2) INFORMATION FOR SEQ ID NO: 20:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 31 base pairs (3) TYPE: nucleic acid (C) STRANDEDNESS: single (D);TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
t9) CLON-: AGLA217 (repeat) ~xi) SEQUENC~ DESCRIPTION: SEQ ID NO: 20:
AA ~ GAGAGAGAGAGAGAGAGAGr.

W O 92/13102 PCT/us92/003~
21005~3 ~ 13 G~-12) INFOP~ATION FOR SEQ ID NO: 21:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 43 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: lineax (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURC~:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA21, ~ down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:
CCTTTAACTTCTCCTGTTCCATGTTTAGTTTTATCGATTTGAG
(2) INFORMATION FOR SEQ ID NO: 22:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 57 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDN~SS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SQURCE:
(B) CLONE: AGLA218 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:
AAGATGAATGGAGGTTGCACCATCTCCTGGAAGGGAAACCGGAC_GGCTA
AACAGTC
(2) INFORMATION FOR SEQ ID NO: 23:
~i) SEQUEN OE CHARACTERISTICS:
~A) LENGTH: 49 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: .single (D) TOPOLOGY: lineax ~ii) MOLECULE TYP~: DNA ~geno~ic) ~iii) HYPOTHrTICA:: NO
(iv) AN-I-SENS-: N_ ~0 92/13102 PCT/U~92/00340 ~l()OS~3 137_ ~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA218 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23:
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGAGAGACAGAG
(2) INFORMATION FOR SEQ ID NO: 24:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 37 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE gOURCE:
: (B) CLONE: AGLA2l8 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
TGTCTTCTGCCTAGACCTCAGGGAATTGTTGAGTTAA
(2) INFORMATION FOR SEQ ID NO: 25:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 130 base pàirs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA22 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:
TGCACAGACTGCGTTTTTCATGTCAAGTGAGATGAGAGCCTCTGûAGGG-TCTGAGCTAAGAACTGGGCTTAAACATTGTATTCAAGACAGGCm--CAGGT
GCGGGGGGCAGAGTTAGGAATCCAGGA...
(2) INFORMATION FOR SEQ ID NO: 2~:

WO 92/131~1 2 1 ~ O a 8 3 PCT/US92/00340 i (i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 56 base pairs (B~ TYPE: nucleic acid (C) ST~ANDEDNESS: sinsle ~D~ TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPQTHETICAL: NO
(iv) ANTI-SENSE: NQ
(~i) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA22 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2~:
GTGTGTGTGTGTGTGTGTGTGTGTGGAGAGAGAGAGAGAGAGAGAGAGAG
AGAGAGAGAGAGAGAGAÇAGAGAGA~AGNNNNNNNNNNAGAGAGAG
t2) INFORMATION FOR SEQ ID NO: 27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 80 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA22 ~down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
CCACTGCCCACTTTCCACCATCAAACTGCCCCTACAGAGTCTAC-TCG C
ACCTAATTCATCACTCTCTCAGCTACA...
(2) INFORMATION FOR SEQ ID NO: 28:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l99 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: lin~ar ~ii) MOLECULE TYPE: DNA ~genomic) ) HYPOTHETICAL: NO
(iv) AN1 ~ - S~NS!: NO

O g2/1310~ 2 1 0 0 5 3 3 PCr/US92/00340 -139- i (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus tvii) IMMEDIATE SOURCE:
(B) CLONE: AGLA220 (up) 3 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
CCTGACTCATTGGAAAAGACCCTGATGCTGGGAAAGACTGAGGGCAGGAG
GAGAAGGGGACAACAGAAGATGAGATACTTGGATGGCATCACCGACTCAA
TGGACATGGGTTTGGGTAGACTCTGGCAGTTGGTGATGGATAGGGAGGCC
TGGTGTGCTGCAGTTCATGGGGTCGCAACAGCCAGACATGACTGAGCGA
~2) INFORMATION FOR SEQ ID NO: 29:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 50 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic~
~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(Yi~ ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA220 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:
AACTGAACTGAACTGAACTGAACTGNNNNN~ NN
(2) INFORMATION FOR SEQ ID NO: 30:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 124 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single . (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
' (vi) ORIGINAL SOURCE:
¦ (A) ORGANISM: Bos taurus tvii) IMMEDIATE SOURCE:
(B) CLONE: AGLA220 ~down) ¦ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30:
AACTCCAGCCCTTCTCCCACCTCGCTGAGTG&CAAACAGGCTC-G--AuGC
AGACAGATGGCATTTAAATTTTGCTTCTGTCACTTGCCAGCCCAA--TCA

W O 92/131~ 2 1 0 ~ ~ 8 ~ PCT/US92/0034~ ~

TGCAAGTTACTGATCCTTGCTGGG
(2) INFORMATION FOR SEQ ID NO: 31:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l0l base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ili) HYPOTHETICAL: NO
(iv) ~NTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (Yii) IMMæDIATE SOURCE:
(B) CLONE: AGLA223 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31:
AAATTATAGACTTCACTTGGGACAATGTCTGTGTTGAATGAATGAGTCAG
TCATTTTCCATGCAAAGATAAATGTCGCATCACCTCTAAATTATGGCATG
G

(2) INFORMATION FOR SEQ ID NO: 32:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ( genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (~ii) IMMEDIATE SOURCE:
. (B) CLONE: AGLA223 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32:
(TC)3TTAGG(AG)2AA(AG)2ACTG(AG)...
(2) INFORMATION FOR SEQ ID NO: 33:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single I ~D) TOPOLOGY: linea_ (ii) MOLECULE TYP~: DNA (genomic) i ~O 92/1310~ PCT/us92/00340 210û~3 (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(8) CLONE: AGLA223 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33:
CCTTTGACTCATTACCGTATAGTGCTCC ~.
(2) INFORMATION FOR SEQ ID NO: 34:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: B7 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA226 ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34:
GATGACTGAAGGGAAATAATTTTCTTCAGTTTTCTGAACCAATNGCTAAA
GAAATGCAGTGTTGTCAGCCAAGCCAAGAATCAGGGA
(2) INFORMATION FOR SEQ ID NO: 35:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) $0POLOGY: linear ~ii) MOLECULE TYPE: DNA ~qenomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
I (A) ORGANISM: Bos taurus ¦ ~vii) IMMEDIATE SOURCE:
ll (B) CLONE: AGLA226 ~repeat) ¦ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 35:
I GTGTGTGTGTGCATATGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAuAG.i W O 92/1310~ 2 1 0 0 5 8 ~ PCT/US92/00340 ~

-i4 ~-GAGAGAGAGAGAGA
~2) INFORMATION FOR SEQ ID NO: 36:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 46 base pairs ~) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (~ii) IMMEDIATE SOURCE:
(B~ CLONE: AGLA226 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36:
GCTTAACAAGCCATGCTGAATGGTCTTATGTGATTTGATTTAACAT
(2~ INFORMATION FOR SEQ ID NO: 37:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 base pairs (B) TYPE: nucl~ic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA227B (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37:
~ AGCTAGCAAGGATCTACTGGGTAGCACAGGAAACTCTACTCAATATTCTC
¦ TAATGACCTACATGG
~ ~2) INFORMATION FOR SEQ ID NO: 38:
¦ (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 73 base pairs (B~ TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linea r 3 (ii) MOLECULE TYPE: DNA (gen~mic) , ~iii) HYPOTHETICAT: NO
.
,~ .

~0 92~13102 PCT/us92/0034~
`~ 21~0~

-1~ 3 -(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA227~ (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38:
AAAAATAATCTAAAAAAGAGGGGATATATGTATATATACATTGTATATAT
ATATTGATATTGATATATATACA
~2) INFORMATION FOR SEQ ID NO: 39:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 62 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA2273 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39:
ATTTATTAGAGCATAGTTGATTTAAAATGCTGTGTTTTTGCTGTACATCA
AAGTGAATCAAT
~2) INFORMATION FOR SEQ ID NO: 40:
(i) SEQUENCE CHA~ACTERISTICS:
(A) LENGTH: 51 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic~
(iii) HYPOTHETICAL: NO
(i~) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA230 (up) (xi) SEQUENCE DESCRIPTION: SE~ ID NO: 40:
AATCCTATG&ACAGAGGAGCCTGu-CAuuGTTACA~TCAT.GuGu~ C~u W O 92/13102 PCTtUSg2/0034~ (~
21005~3 A
(2) ~NFORMATION FOR SEQ ID NO: 4l:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA230 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41:
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 42:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l05 base pairs (~) TYPE: nucleic acid (C) STR~NDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA230 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 42:
TGTACTGGTTCTCTGCAGGCCCTGTGTCCACTGCGAAGACAGAG~TCCAT
GGCTTCTTGAGGTTCTAGGAGGAGTCTATTTGACTTGAGATGCGATTTTT
CCACA
~2) INFORMATION FOR SEQ ID NO: 43:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 120 base pairs ~B) TYPE: nucleic aci~
(C) ST~ANDEDNESS: single (D) TOPOLOGY: linea (ii) MOLECULE TYPE: DNA (geno,~

~10 9~J~310~ 2 1 0 ~ ~ ~ 3 PCI/US~2/00340 (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA232 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 43:
AAAATCCACATGCCTTTGCAAATACCTCCTGACCAGTCCCCCTCGGTTCT
CCACAGCCCTTGCCACACCGATGCAGACGTACACGACGATGGATAAAAAC
TCGATTTATCCATCATCGAA
(2) INFORMATION FOR SEQ ID NO: 44:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 31 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: 11near (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA232 ~repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 44:
A&AGAGAGAGAGAGAGAGAGAGAGAGAGAGA
~2) INFORMATION FOR SEQ ID NO: 45:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
¦~vi) ORIGINAL SOURCE:
I(A) O~GANISM: 30s taurus ¦~vii) IMMEDIATE SOURCE:
~~B) CLON~: AGLA232 (down) i(xi) S~QUENC- DESCRIP-ION: S_~ ID N~

W O 92~l3lo~ PCT/US92/00340 ~.

GAATTCCAGCGAAATGGTTCTACATTTGCTAGGTGTCCT
~2) INFORMATION FOR SEQ ID NO: 46:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 80 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HY20TH-TICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORG~NISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA233 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 46:
TGATAGAAATTCCCAGATGATAACTCAGATGTGAAAGCACTGTGCAAACA
TCCACGTAGCATAAATACCTCTGAGCATAC
~2) INFORMATION FOR SEQ ID NO: 47:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 base pairs (~) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
I (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA233 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 47:
~AG)+++
(2) INFORMATION FOR SEQ ID NO: 48:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 54 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single .~j (D) TOPOLOG~: linear (_i) MOLECUL- TYPE: DNA (genomuc~

/0 92~1310~ PCT/us92/0034n 2100'J8~

_l~7-(iii) HYPOTHETICAL: NO t ~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE: ,~
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA233 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 48:
ÇGCCCAGGGTTGGTCCCAAATGGCATGAACAGCCAATAGTGTCATCAGGC
ATTA
(2) INFORMATION FOR SEQ ID NO: 49:
(i~ SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRA~DEDNESS: single (D~ TOPOLOGY: linear (ii) MOLECULE TYPE~ DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA234 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 49:
CTTGCTTTAGATTTATTTATTCATTTGTGATGCA
(2) INFORMAT~ON FOR SEQ ID NO: 50:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ¦ (ii) MOLECULE TYPE: DNA (genomic) ¦ (iii) HYPOTHETICAL: NO
¦ (iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B~ CLONE: AGLA23~ (repea~) i (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50:
GTGTGTGTGTGTGTG~GTGTGTGAGGAGAGAGAGAGAG~GAGr~G.;uAG.;G

WO 92/1310~ 2 1 0 0 ~ 8 3 PCI`/US92/00340 ~ ~

AGA~AG
(2) INFORMATION FOR SEQ ID NO: 51:
Ii) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTH-TICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(~) CLONE: AGLA234 (~own) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51:
AAATCTTTGTATCCCCACCATTATACACAAAGTTTTTCCATGGAGTACAT
GCTCACCTGCTAGTG
~2) INFORMATION FOR SEQ ID NO: 52:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 50 base pairs (B) TYP~: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (g~nomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 80s taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA243 (up) ¦ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 52:
! AGCTTAGGCAAATCAGTGTTACATCTCCATTTTATTTAGAAGATAGCAGG
(2) INFORMATION F~R SEQ ID NO: 53:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linea-(ii) MOLECULE TYPE: DNA (genomic) (iii~ HYPOTU.ETICAL: NO
J

~0 92/13102 2 1 0 ~ PCT/US92/00~40 ~lv) ~NTI-S NSE: NO
(~i) ORIGINAL SOURCE:
~A) ORGANISM: Bos ~aurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA243 (repeat) (xi~ SEQUENCE DESCRIPTION: SEQ ID NO: 53:
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 54:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 83 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) ~YPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
tB) CLONE: AGhA243 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54:
TGCATAGGCAGGCCCAGGTGGAGGAAAAAACATATAAAAGGAGGAGCCAA
AGCGCTTTCTCACGGACTCTCTCCCGCGTGCAT
(2) INFORMATION FOR SEQ ID NO: 55:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 92 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA247 (up) ~x~) SEQUENCE DESCRIPTION: SEQ ID NC: ~_:
ATAAA-_AAGGATCAG-A~.-A-T--CTG-AAAGG~ -A-AA-.~;-A-TAGAA.TTGTAGGTCACATA-AGATT_TA.CACA-A~u---W O 92/13102 PCT~US92/0034~ ( 2100~3 --15C)-(2~ I~EO~MATION FOR SEQ ID NO: 56:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA247 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56:
TTTTTTTTTTTTTTTTTTTT
(2) INFORMATION FOR SEQ ID NO: 57:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
tA) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA247 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 57:
ATCAAGGATGGATTTACATTTTAAAGCATTGTTT
(Z) INFORMATION FOR SEQ ID NO: 58:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 38 base pairs ~B) TYPE: nucleic acid (C) STRANDE~NESS: single (D) TOPOLOGY: linear (ii) MOLECUL~ TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-S~NS-: NO

~O 92/1310~ ~ 1 0 0-~ ~ 3 PCT/US~2/oO34~

~vi) O~lGINAL SOURCE:
(A) ORGANISM: 30s taurus ~vii~ IM~EDIATE SOURCE:
(B) CLONE: AGLA248 (up) (xi1 SEQUENCE DESCRIPTION: SEQ ID NO: 58:
AACCAGTAGGCAAAACTCAATGACTCGTAAGAGAATGG
12) INFORMATION FOR SEQ ID NO: 59:
(i) S!QUENCE CHARACTERISTICS:
(A) LENGTH: 22 base pairs (3) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE~ DNA (genomic) tiii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA248 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 59:
ANAGAGAGAGAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 60:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 152 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: si~gle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA Igenomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA248 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 60:
AACCCTGATGGATGCTCACCTACAGACCCTGATGTATTTCT-CCTCAGAA
ATTTCTCCATATTAGGGGTGTCATCCACAACTG.CACTGTACCCAAAT-C
CTTATCACCATTACTACAGGAGAGAAAACCTTTTCTTTTACTGATTGTAT
G~

W O 92/1310~ 2 1 0 0 ~ 8 3 PCT/US92/0034o ~-~s~-(2) INFORMATION FOR SEQ ID NO: 61:
(i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 22 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (i~i) HYPOTHETICAL: NO
liv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA254 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 61:
AGCTGCTTGGCACAGGCAAAAA
(2) INFORMATION FOR SEQ ID NO: 62:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 30s taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA2 54 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 62:
! (AG)+++
(2) INFORMATION FOR SEQ ID NO: 63:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 119 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOT~ETICAL: NO
(iv) ANTI-SENSE: NO

10 92/1310~ 2 1 Q O ~ ~ 3 PCTtUS92tO034o 53 - ;
~vi) ORIGINAL SOURCE:
- ~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA254 ~dow~) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 63:
TTGCCTCCTTTATTACAGATTAATTGACCATAAhTGTGTGGATTAATTTC
TGGACTCTGTATTCTGTGCCATTAATCTCTGTGTCCTTTTTTTTTTTCTT
TTGTTCCAGTGCCATACTT
~2) INFO~MATION FOR SEQ ID NO: 64:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 8Q base pairs (3) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA255 ~up~
~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 64:
AAATCTCAATTTCCTCATCGGTAAAATGGGGATAATAATTCCTGATTTGC
AGGGTTGTTGTGGTAATTAAATGAAACTGG
(2) INFORMATION FOR SEQ ID NO: 65:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGT~: 5g base pairs ~) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLON~: AGLA255 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 65:
TGTGTGTGTATGTGTGTGTGTAAAAGAGAGAGAGATTTCGAGAGAGAAAG
AGAG

W O 92~13102 ~ 1 0 0 ~ ~ 3 PCT/US92/oO34~

_ (2) INFORMATION FOR SEQ ID NO: 66:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 65 base pairs (B) TYPE: nucleic acid (C~ STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURC~:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B~ CLONE: AGLA255 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 66:
CACCGGCAGGATGGGAAAGTGCACAGAGAACGGAAGACCATTAAACACCT
CCTCCATGTCAGGGG
(2) INFORMATION FOR SEQ ID NO: 67:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l9 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: N0 (vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA257 ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 67:
AGCTAGATTAACACCAAAA
~2) INFORMATION FOR SEQ ID NO: 68:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

'O 92/1310' PCT/USg2~0034n 210~3 ~ss-(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE AGL~257 (repeat) (xi) SEQUENCE DESCRTPTION SEQ ID NO 68 AGAGAGAGAGAGAGAGAGAGhGAGAGAGAGAGA
(2) INFORMATION FOR SEQ ID NO 69 (i) SEQUENCE CHARACTERISTICS
(A) LENGTH: 65 base pairs (B) TYPE nucleic acid ~C) STRANDEDNESS single ~D) TOPOLOGY linear ~ii) MOLECULr TYPE DNA (genomic) (iii) HYPOTHETICAL NO
(iv) ANTI-SENSE NO
lvi) ORIGINAL SOURCE
(A) ORGANISM Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE AGLA257 (down) (xi) SEQUENCE DESCRIPTION SEQ ID NC: 69:
TTGGTGTGTAATTGTTCATACTGATCTCTTAGAATCNTTTGTAATTCCAT
GTATCAGTTGTAATG
(2) INFORMATION FOR SEQ ID NO 70:
(i) SEQUENCE CHARACTERISTICS
(A) LENGTH 90 base pairs (B) TYPE nucleic acid (C) STRANDEDNESS single (D) TOPOLOGY linear (ii) MOLECULE TYPE DNA (genomic) (iii) HYPOTHETICAL NO
(iv) ANTI-SENSE NO
(vi) ORIGINAL SOURCE
(A) ORGANISM Bos taurus (vii) IMMEDIATE SOURCE
(B) CLONE AGLA258 (up~
j (xi) SEQUENCE DESCRIPTION SEQ ID NO 70:
CAGAGACTTTTCATTCTGAGGAAAAACCTCCAGAAAGCCATATTC-ATCT

CTGGTGGCCCATATTCCATCTCTGCTGACTTCTCATAAAC
~ (2) INFORMATION FOR SEQ ID NO: 71:

W 0 92/1310~ 210 0 5 8 3 pcTtuss2/oo34n (i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 86 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTYETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA258 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID MO: 71:
TGTGTGTGAGAGAGAGAGAGAGCAAGAGAGACAGAGAGACAGATGAGAGA
A&AGAGACAGAGAGGCATACAGTAGAGTTTGAATAT
~2) INFORMATION FOR SEQ ID NO: 72:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 134 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MO~ECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURC~:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA258 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 72:
CCTCAGAACACAGAGAAGAGAAAACTGGGTCCTTGATGGGAAACAGCAGG
GGACTGCGGGGTATGGACAATTAATTTTGAAATCCAGGACAATGACAGAA
TTGTTGCTTCATTGTGGCTTTCCCAATATTCAAA
(2) INFORMATION FOR SEQ ID NO: 73:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 147 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomi~) (iii) HYPOTHETICAL: NO

YO 92~1310~ PCT/US92/00340 21033~3 ~S7 -(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA259 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 73:
AACCAGATCTCCTGAGTCTCTTGCATTGGCAGGTGGATTCTTTCCCACTT
AGCCACCTGGAAGCCCTGCTTCCATCCATGGGATTTTCAAGGCAAGAGTA
CTGGAGTGGGGTGCCATTGCCTTCTCCAGTAAAGAGTAAATAAC...
(2) INFORMATION FOR SEQ ID NO: 74:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 46 base pairs ~8) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMED IATE S OURCE:
(B) CLONE: AGLA259 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 74:
AGAGAGAGGGAGAGCGGGAGAGCGGGAGAGAGAGAGAGAGAGAGAG
~2) INFORMATION FOR SEQ ID NO: 75:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA259 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 75:

GTCAGGATATTGCAATTTTAAGTGTCCTTTAGmT

W O 92J~3~02 2 1 0 0 a ~ 3 PCT/US92/00340 ;'~`

-15~3 ~2) INFORMATION FOR SEQ ID NO: 76:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTHo 168 base pairs ~B) TYPE: nucleic acid (C) STRANDFDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HY~OTHETICAL: NO
~iv~ ~NTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (v~i) IMMEDIATE SOURCE:
~B) CLONE: AGLA260 ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 76:
GTATGACTAAACACTCCCATTGCTTTATTAATGCCCTGTGAATGGGTTAA
GCATTTTGGACAAAATTCACTAATGTTTTTAACCGACCAGAGATTACATA
TAATCATTCAGATAAGTCAATCAGCAATCCTCAATTTTACCTTCTGACCA

(2) INFORMATION FOR SEQ ID NO: 77:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 52 base pairs ~B) TYPE: nucleic acid ~C) STRAN3EDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA260 kepeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 77:
GAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GA
(2) INFORMATION FOR SEQ ID NO: 78:
(i) SEQUENCE CHARACTERISTICS:
(A~ LENGT~.: 123 base pai-s (~) TYPE: nucleic acid . (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE T~PE: DNA ~ge~o~ic) `~0 92~13102 PCT/US92/00340 21005~3 ~59-~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos ~aurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA260 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 78:
CCTGGAGGCCTTTGGACTTAATTGCAACATCAGTTCTGCCCTGGGTCTCA
AATCTGCCGGCCTGCCCTGAAGATTTTGGACTTACCATTTCTC~GTAATT
ACATTAGCCAGTTCCTTAAA...
(2) INFORMATION FOR SEQ ID NO: 79:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 74 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear lii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) I~MEDIATE SOURCE:
(B) CLONE: AGLA267 ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 79:
AGCTTGGTGTTCTATAATCCATGGGGTTGCAAAAGGATCGGACATGGCTT
AGCAACTAGACAACTAAACAG...
~2~ INFORMATION FOR SEQ ID NO: 80:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 44 base pairs ~B) TYPE: nucleic aGid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~ii) IMMEDIATE SOURCE:
(B) CLONE: AGLA267 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NC: 8C:

~ y W O 92/1310~ 2 1 0 0 5 8 3 PCT/US92/Oo340 [ ~

~o AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 81:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 138 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linea~
(ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vli) IMMEDIATE SOURCE:
~B) CLONE: AGLA267 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 81:
CCTGAATCTCGGTTCCCCTTCTCTTCAAAGTAGTGACTTTCAGCCCAGAT
CCCTGCAATTTCTTCAAAGTTCACATTACTTCCTCACAAAGTATGATTGG
ACATAGGAAGAACTTTTTCTGAGTGTGTATTGGTG...
~2) INFORMATION FOR SEQ ID NO: 82:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) NYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA269 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 82:
CTTTCAATGTATTTGCTTATTTGTTCAACTG
(2) INFORMATION FOR SEQ ID NO: 83:

(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: ~NA (genomic) YO 9~/13102 PCT/usg2/0034n 21t)0~3 ~iii) HYPOTHrTICAL: N0 (iv) ANTI-SENS-: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus tvii) IMMEDIATE SOUKCE:
(B) CLONE: AGLA269 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 83:
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGTGTGTGTGTGTGT~G
TGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 84:
(i) SEQUENCE CH~RACTERISTICS:
(A) LENGTH: 147 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) XYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURC~:
(B) CLONE: AGLA269 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 84:
CCT5ACACTAGTAGATTTGAAACCCATt;TGTGTAATTTGCTATTmTCAGG
TTAAAGAATAAAAGACTTGTACACAAATACTGTACACTAATTGCCAAACT
TGTTTTTCCATAGAAGTGTGGGTTCAGAAACCTCTCCAAGCCCCAAA
(2) INFORMATION FOR SEQ ID NO: 85:
(i) SEQUENCE CHARACTERISTICS:
(A),LENGTH: 84 base pairs (B3 TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: llnear (ii) MOLECULE TYPE: DNA (genomuc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA2,2 (up) W O 92/1310' 210 ~ 3 8 3 PCT~US92~00340 j ~ 6 2-(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 85:
CTTGTTTTTTTCTATTCAGTTTTATGAGTTCTACTTTGAATATTAACTCT
TTATCACATATGATTTGCAAAATTTCCCATTCTA
~2) INFORM~TION FOR SEQ ID NO: 86:
~i) SEQUENCE CHARACTERISTICS:
A) LENGTH: 20 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLO&Y: linear (ii) MOLECULE TYPE: DNA (genomic) (ili) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos tauxus ~vii) IMMEDIATE SOURCE:
~3) CLONE: AGLA272 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 86:
TCAGTTCAGTTCAGTTCAGT
(2) INFORMATION FOR SEQ ID NO: 87:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 5l base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii~ MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMæDIATE SOURCE:
(B) CLONE: AGLA272 (down) xi) SEQUENCE DESCRIPTION: SEQ ID NO: 87:
CCCGGCATGCTGCAATTCATGGGGTCGCAAAGAGTTGGACACA~_TGAGT
G
(2) INFORMATION FOR SEQ ID NO: 88:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 64 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: sin~le ~D) TOPOLOGY: linear '~O 92/1310~ PCT~US9~00340 2100'3~3 (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ( Yii ) IMMEDIATE SOURCE:
(B) CLONE: AGLA280 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 88:
AGCTCTTATGCTCAAAGTTGTCCTCAGAGAGGGGTCAACTGGGATTCA~
TATAGGAACAGCAA
(~) INFORMATION FOR SEQ ID NO: 89:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECUL~ TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA280 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NC: 89:
AGAGAGAGAGACAGAGAGAAACAGAAAGAGACAGAGAGAGAGAGACAGAG
AGAGAGAGAGACACACAGAGAGAACACACACACA
(2) INFORMATION FOR SEQ ID NO: 90:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 42 base pairs ' (B) TYPE: nucleic acid ¦ (C) STRANDEDNESS: single (D) TOPOLOGY: linear I (ii) MOLECULE TYPE: DNA (genomic) 3 (iii) HYPOTHETICAL: NO
tiv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ' (vii) IMMEDIATE SOURCE:`
(B) CLONE: AGLA280 (dowr.

f W O 92/13~ 2 1 0 0 5 8 3 PCT/US92/00340 f`~

~ 6 ~-(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 90:
GAATTCAGAAGGTGTGGGTGGAGGAGACAGACCAAAAACACT
(2) INFORMATION FOR SEQ ID NO: 91:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 46 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (3) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA284 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 91:
TGCAGTTTCATAACTGTACAGCAGTCATGGACAGGTCAAATCCAGG
(2) INFGRMATION FOR SEQ ID NO: 92:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 3 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:.
. (B) CLONE: AGLA284 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 92:

... .
(2) INFORMATION FOR SEQ ID NO: 93:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 32 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~0 92/131~ PCT/U~92/00340 2laos~.¢3 (ili) HY?OTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA284 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 93:
GTACCAGTGACATTCAGAGGAGCACCAACAAT
(2) INFORMATION FOR SEQ ID NO: 94:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 base pairs (3) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (Yii) IMMEDIATE SOURCE:
(B) CLONE: AGLA285 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 94:
CTTAAAAGACCAAGGCACAAAAGGCTTCCTGCCAATCCCATTAAAGTTGA
AGGCAGAGGATTTTG
(2) INFORMATION FOR SEQ ID NO: 95:
(i) SEQUENCE CHARACTERISTICS:
j ~A) LENGTH: 49 base pairs I ~B) TYPE: nucleic acid ! (c) STRANDEDNESS: single ' (D) TOPOLOG~: linear (ii) MOLECULE TYPE: DNA (genomic) i (iii) HYPOTHETICAL: NO
i (iv) ANTI-SENSE: NO
, ~vi) ORIGINAL SOURCE:
t (A~ ORGANISM: ~os taurus (vii) IMMEDIATE SOU~CE:
(B) CLONE: AGLA285 (repea:) ~xi) SEQUENCE DESCRIPTION: SEQ 'D NO: 98:
AAAA~AAA~A~AA~GAGAGAGAGAGAGAGAGAAAGAGAG~GAGAGAG.i W O 92/1310' 210 0 ~ 8 ~ pcT/us92/~o34n -(2) INFORMATION FOR SEQ ID NO: 96: 3 ~i) SEQUENGE CHARACTERISTICS:
(A) LENGTH: 62 base pairs tB) TYPE: nucleic acid ~-~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos tausus ~vii) IMMEDIATE SOURCE:
(B) CLONE: AG~A285 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 96:
AGCACCCTTCTTGACCTGCAACAGGCATGCAGGGNNTCATTTTGATNAGC
ACTGAACAAATT
(2~ INFORMATION FOR SEQ ID NO: 97:
(i) SEQUENOE CHARACTERISTICS:
(A) LENGTH: 54 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) NOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA29 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 97:
ACAGGAAGCCGAGTGAGATATGTAAGCCAAAACTCAGACAGGTGGAGTAG
AGGA
(2) INFORMATION FOR SEQ ID NO: 98:
(i) SEQUENCE CH~RACTERISTICS:
(A) LENGTH: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO

~O 92/13102 ' 2 1 0 ~ ~ ~ 3 PCT/US92/~0340 ~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA29 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 98:
GTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 99:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 47 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) I~MEDIATE SOURCE:
(B) CLONE: AGLA29 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 99:
TTCACATTACAGCCTGTGTGAATGTCCTCTATGACCTGCCTGGAGGA
~2) INFORMATION FOR SEQ ID NO: l00:
(i) SEQUENCE CXARACTERISTICS:
(A) LENGTH: 129 base pai~s (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA29l (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: l00:

W O 92/13102 2 1 0 0 ~ 8 3 PCT/~S~2/00340 ~

CTGGccAGTTTTGAAGGATAAAATAAGTGTTTTAGcAAAcAAccAAAAT~
CCTTAATGCCATTGTCAAAGAAACACAGTATTTTCTCTCAAATGATGAAT
ATGCTTCTATGAAATTACTTTGCTGG...
~2) INFORMATION FOR SEQ ID NO: l0l:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sin~le (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA25l (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: l0l:
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 102:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 67 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA29l (down) ~xi) SEOUENCE DESCRIPTION: SEQ ID NO: 102:
GGCTGTTCTGCCTTTTCCAACAAGTTAGACTAAACTTTCTAGGACTATTC
TATGCATGCCTCTCCAG
~2) INFORMATION FOR SEQ ID NO: 103:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 base pai-s (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linea_ 40 92/13102 PCT/u~92/~0340 210~3~3 ~l~g- '.
(ii) MOLECULE T~PE: DNA (genomic) ~.
(iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi~ ORIGINAL SOURCE:
(A) ORGANISM: 30s taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA293 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 103:
AGGGCAAGGGAGAGAAACTCAACCCAAGACAACTCAAGTCAAAGGACCAA
GACTGAGAACCCA...
(2) INFORMATION FOR SEQ ID NO: 104:
(i) SEQUENCE CHARACTERISTICS:
(A~ LENGTH: 72 base pairs (B) TYPE: nucleic acid (C) 5TRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA293 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 104:
AGAGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGT
GTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 105:
(i) SEQUENCE CHARACTERISTICS:
(A)iLENGTH: 83 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA293 ~down) W O 92/1310~ 2 1 0 0 5 8 3 PCT/U~9~/00340 ~.` `

~ 7C -(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 105:
CTACCAATGACTTTATTCTCCACCTAGCAGAAAAGTCCAGTCTGAAATTG
GAGGCAATGAGGCAAATATTCAAAGAGA~N...
(2) INFORMATION FOR SEQ ID NO: 106:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 75 base pairs (B) TYPE: nuclelc acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA296 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 106:
CTGGTTAAATGAAATTCCATAAACTGCTCACAAATTG~AAAAAAACTTAC
ACTTGGATAACTAAATCATGTAATA
(2) INFORMATION FOR SEQ ID NO: 107:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 30S taurus (vii) IMMEDIATE SOURCE:
(B1 CLONE: AGLA296 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: l07:
GTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGA
~2) INFORMATION FOR SEQ ID NO: 108:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 67 base pai-s ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linea ~0 92/1310' PCT/~'s92/0034n 210~5~3 - I 7 l--~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) I~MEDIATE SOURCE:
(B) CLONE: AGLA296 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 108:
GTAGAAAATTAGGTGTATCAAGGATTATTACATGAGCGT...CTCAAAAA
TAGTAAGTACCCACTCA
(2) INFORMATION FOR SEQ ID NO: l09:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 58 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA298 (up) (xi) SEQUENCE DESCRIPTION: SE2 ID NO: l09:
CAGTTCCTGATGAGAATCCTTTCCTTGCTTGTGGATGTCTACTGTGTCCT
TACATGGC
(2) INFO~MATION FOR SEQ ID NO: ll0:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii~ HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIA~E SOURCE:
(B) CLONE: AGLA298 (repeal) W 0 92/13102 210 0 a 8 3 PCT/US92/0034 -17~-(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 110:
AGAGAGACAGAGAGAGAGAGAGAGA
(2) INFORMATION FOR SEQ ID NO: 111:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 64 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: lihear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA298 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 111:
CGTAAAGGTAGTGCCCTGAACCCATAGACCTAGTTTCCTTACATAAAAAG
GAACA&AAACCAGA
(2) INFORMATION FOR SEQ ID NO: 112:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 59 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA2g9 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 112:
AGTAGGAAGAAAAACCAGAACTAAGGAGTTGGCTGTTGTATGAACATGAT
GTAGAGGAG
(2) INFORMATION FOR SEQ ID NO: 113:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 57 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: si~gle (D) TOPOLOGY: linear /0 92J1310~ PCT/uS92/0034n 210~3~ `
- 17;~
(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA299 (repeati (xi) SEQUENC~ DESCRIPTION: SEQ ID NO: 113:
AAAGCAAAAAA~AA~A~AAAAAGAGAGAGAGAGAGAGAGACAGAGAAAG
AGAGAGA
(2) INFORMATION FOR SEQ ID NO: 114:
(i~ SEQUENCE CHARACTERISTICS:
~A) LENGTH: 67 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA259 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 114:
AAGATTTTTGAGAAAAATGTTTATTC~ACTCGAAGTTGATCTTAAGACAC
AGCCATTAACAAACCTT
(2) INFORMATION FOR SEQ ID NO: 115:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 120 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA300 (up) WO 92/1310~ 2 10 0 ~ ~ 3 PCr/uSs2/0034n ~` `

_ 1?~- , (xi] SEQUENCE DESCRIPTION: SEQ ID NO: 115:
TCTCCAAGTCTGTCATTGCCTTAGGGTGCTCCAGCATCCATTCCATTTTG
CAAAATATTAATTAGCCCTTTCATTTTGGACACAGTAACTGCTGTCTACC
TATTCTCAC~GTGTTAA...
(2) INFORMATION FOR SEQ ID NO: 116:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 58 base pairs ~B) TYPE: nuc~eic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANIS~: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA300 (r~peat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 116:
AGAGAGAGAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
AGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 117:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 76 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: li~ear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA300 ~down) 5xi) SEQUENCE DESCRIPTION: SEQ ID NO: 117:
AGCTTTCTTCACAGTCCAACTCGTGGGTGAGTAGGTAATTACAAA~GCTG
CTGTTTGTCATCACAGTTGTTATAGA
~2) INFORMATION FOR SEQ ID NO: 118:

~i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 62 base pairs (B) TYPE: nucleic acid 'O 92/~310~ PCT/US92/~0340 210U~g3 ` 17~
(C) ST~ANDEDNESS: single (D) TOPOLOGY: linear (ii) MO~ECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA33 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 118:
TGCAGGGAAGTCCCTACAATATTCTTTAACCGAGTGTCCATCAAAGCTGA
TCAATCGTCATG
(2) INFORMATION FOR SEQ ID NO: 119:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 48 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: AGLA33 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 119:
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
(2) INFORMATION FOR SEQ ID NO: 120:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 54 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:

W O 92~13102 21~ O ~ 8 3 PCT~US92/0034~ ~.

_17 ~-~B) CLONE: AGLA33 (down) ~xij SEQUENCE DESCRIPTION: SEQ ID NO: 120:
ATGTAGCTAGACATATTTCTTAAATTTCTCGTTGCAGGACAGTTTCTCAG
ACTT
(2) INF0RMATION FOR SEQ ID NO: 121:
(i~ SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi~ ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) I~MEDIATE SOURCE:
(B) CLONE: AGLA8 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 121:
AAAGTGATGCATCTTTTGAAACAAAAGTTTCCATCTTTATACTTGCCACC
AAGCCATTTTATCT
~2) INFORMATION FOR SEQ ID NO: 122:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 40 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: N0 ~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) I~MEDIATE SOURCE:
(B) CLONE: AGLA8 ~repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 122:
TGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAG
~2) INFORMATION FOR SEQ ID NO: 123:

~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 57 base pairs (B) TYPE: nuclelc acid (C) STRANDEDNESS: single YO ~2/13102 PCT/US~2/00340 2100~
i -177 _ (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO ', (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: AGLA8 (dow~) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 123:
GAGGGGCCTGGAGNGTATAGAACATGGGGTCACAAAGAGTCAGACACAAC
TGAGCAG
(2) INFORMATION FOR SEQ ID NO: 124:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 121 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLQGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHSTICAL: NO
(iv) ANTI-SBNSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
B) CLONE: GBFSH ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 124:
CTTTATGGGGTGGTAAATGAGTTTGACCAAAATCCACTTATCACATCTTC
TTGGGATATAGACTTAGTGGCATGATATTGAAATAACTTAAACAGAAGTA
ACAACTTTTCCCTCAGTGCAA
(2) INFORMATION FOR SEQ ID NO: 125:
~i) SEQUENCE CHARACTERISTICS:
~A~ ~ENGTH: 53 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCS:
(A) ORGANISM: Bos taurus WO 92/1310~ 2 1 0 0 ~ 8 3 PCr/US92/Q034(1 (`--~8-(vii) IMMEDIATE SOURCE:
(B) CLONE: GBFSH (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 125:
ATATATATATATATATATATATATATATATATATATATATTTTTTTTTTT ~ S
TTT
(2) INFORMATION FOR SEQ ID NO: 126:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 120 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPF: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORG~NISM: 30s taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: GBFSH (dow~) Ixi) SEQUENCE DESCRIPTION: SEQ ID NO: 126:
AGACATCTCTCCACCTTGAAGCCTATATATTTTGCACAAGTCACAGTTTC
TAAGGCTACATGGTTTGTACATAAGAACTTTAATCCTTGTAGA~TAGGTT
TTTTTGTAGCACTTTCTAGT
(2) INFOR~ATION FOR SEQ ID NO: ;27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 57 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
- (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: GBIRBP (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 127:
TAGACCTATTGCCATACAGACAAATGTATGATCACCTTCTATG_-TCCA-TCTAGGA

(2) INFORMATION FOR SEQ ID NO: 128:
(i) SEQUENCE CHARACTERISTICS:

'O ~2/1310' PCI/US92/00340 '2 1 a ~ 3 -l ~9--(A) LENGT~: 85 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii1 HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~3) CLONE: GBIRBP (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 128:
CTCTCTCTCCTCCCTCTTCTCTCTCTCTCACATACATACACACACACACA
CACATATATATACATGCATATATATTATATATATA
(2) INFOR~ATION FOR SEQ ID NO: 129:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 93 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORI&INAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: GBIR~P (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 129:
CCCTAAATACTACCATCTAGAAGATACTATTGTTTCCCTAAATACTACCA
TCTAGAAGATACTATTGTTTGCTTTAGGTAATCATCAGATAGC
(2~ INFORMATION FOR SEQ ID NO: 130:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 164 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:

W O 92/1310~ 2 1 0 ~ S 8 3 PCT/US~2/0034~ ~``

t.
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: GBKCAS ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 130:
TCATAATGTAAACCACTTTGCCCAGAGCTTGGCATATATAAAAAGTGCCT
CAGTAAATGTTGCACTTTATAAGCACCACAGCTAGACCAGTTTCATAGAG
AGCTTTGACATACAATAGACAAGCATACTAGACACAAAGAAAAAAAACTG
TATATCTCTTTCCA
(2~ INFORM~TIQN FOR SEQ ID NO: 131:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 53 base pairs ~3) TYPE: nucleic acid (C) STRANDEDNESS: ingle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: GBKCAS (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 131:
ATATATATGAGTGTGTATATGTGTGTGTGTGTATATATATATATATATAT
ATG
(2) INFORMATION FOR SEQ ID NO: 132:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 174 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA (~enomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: GBRCAS ~down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 132:

CTGATTTTTAACGGATAAAACTAAGCAGAAAAAAAACCCCAACATATAAA
CCCAGGAATCCACATCACATTATTTTTACTATTTTGCTTGAAT-A&TTTA
GATAAAATGCACCCTTAACCTAATCCCTAGATAAATAAAAT.~;AAACAG

YO 92/131D2 PCT/Us92/00340 210~3 TTACAAACATGTGGTGAGAATAAA
~2) INFORMATION FOR SEQ ID NO: 133:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 32 base pairs (B) TYPE: nucleic acid (C~ STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus - (vii) IMMEDIATE SOURCE:
~B) CLONE: GBPRLGR ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 133:
GTCTAGAATTTAGTTTTACTGCATTTACCAGA
~2) INFORMATION FOR SEQ ID NO: 134:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 29 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linaa~
~ii) MOLECULE TYPE: DNA tgenomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
(~) CLONE: G~PRLGR ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 134:
GTTTGTGTGTGTGTGTATGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO~ 5:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 49 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLEGULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

W O 92/13]0~ 210 ~ ~ 8 3 pcT/uss~oo34n ~ ~`

-182- ;
tiv) ANTI-SENSE: NO
(vi~ ORIGINAL SOURCE:
(A~ ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: GBPRLGR Idown) `.
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 135:
CTAACTTTAGGGAGGTCATACTTGGGGAAGTGACAGTGGGTTTCAAGGG
(2) INFO~MATION FOR SEQ ID NO: 136:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 177 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sinsle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG1 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 136:
TTTGAGTAACTGAAGTATATTGTACTAGTAGTATCAAGTCAGAGACTTGC
CCTTTTTAAAGTGAACTAGGATTTAAAAATGTTAAATGAATAGAGAAGTG
CAGGATTATAAGGTATATATGAATTGGGTTTTGCATAACAAAGAAAAGTA
GCTGAGAAGTGAAGGAGATGGTGATTT
~2) INFORMATION FOR SEQ ID NO: I37:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 49 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETIGAL: NO
(iv) ANTI-SENSE: NO
fvi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG1 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 137:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGAGAGAGAGA

'O ~2/1310~ PCT~Us92/00340 2la~

- l 83 -(2) INFORMATION FOR SEQ ID NO: 138:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 58 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOUR~:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTGl ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 138:
TACAACTTACATTCTAGTCCACTGGCAGTAGTTAGTTGTCCCTAGTGCTC
TCATGTGG
(2) INFORMATION FOR SEQ ID NO: 139:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 40 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE- DNA ~genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
I (vi) ORIGINAI SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTGll (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 139:
GCATTACTGTATTCTTTTTTATGGCTCAGTAATATTCCTC
(2) INFORMATION FOR SEQ ID NO: 140:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 44 base pairs I (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear . (ii) MOTECULE TYP~: DNA (genomi-) (iil) HYPOTHETICAL: NO
i 21~0a~3 W O 92~13~0~ PCT/US92/00340 ~ ;

(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE: S
(B) CLONE: MGTCll (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 140;
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 141:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 79 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORG~NISM: Bos taurus (vii) IMMEDIATE SO~RCE:
~B) CLONE: MGTGll ~down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 141:
GGCCTTGATTTCCTCATCAGTAGAGTGAGATAAAGACTGTACCTAGCTCC
ACTGATAAATAAATGAGTAAAGAAGATGT
(2) INFORMATION FOR SEQ ID NO: 142:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 73 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ( genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG13A (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 142:
CAGCACCGTGCTGTCCATGGTACAAAACCTGACTATTCTCAGA-TTTCTT
TATTCTGTGTGGAACATCTCCTT

` 1092/l3l0~ Pcr/uss2~0034n 210~58~

_l~ 5~
~2) INFORMATION FOR SEQ ID NO: 143:
(i~ SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sin~le (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(~) CLONE: MGTG13A (repeat) (xi) SEQU~NCE DESCRIPTION: SEQ ID NO: 143:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCACGTGTGTG
TGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 144:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 89 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG13A (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 144:
AAAGAGTCGGACATGACTGAGTGACTAAGCCAGGACACGCCCACGTGCCA
GCATGCCCTCACTTGCCCTAGTGCATGCCCCCGCCCTCC
~2) INFORMATION FOR SEQ ID NO: 145:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 77 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single.
(D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic~

~iii) HYPOTHETICAL: NO

W O ~2/131~ 2 1 0 0 5 8 3 PCT/US92/00340 ~~

-18 ~ -tiV) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: 30s taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTGl3~ (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 145:
ACGTCCTCTAGATTCATCCCTGTTGTTGCAAATTGAAGGATTTCTTCTTA
TGGTTGAATAATATTCCGTTATATAAA
(2) INFORMATION FOR SEQ ID NO: 146:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nuclelc acid (C~ STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTGl3B (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 146:
TGTGTGTGTGTG~GTGTGTGTGTGT
~2) INFORMATION FOR SEQ ID NO: 147:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 61 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (qenomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTGl3B (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14/:

TTGTTTATGGGGTCACAAAGAGTCGGACATGACTGAGTGACT~.GTTTSA
CCTATAATATT

~o 92/1310~ ~ l o o ~ 8 ~ Pcr/uss2/oo34o ~2) INFORMATION FOR SEQ ID NO: 148:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 199 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single '-(D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG3 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 148:
AGCTTACTTTGAAAATTTGACACAGGGAGTAATNANGATTTCTTTTGAAA
TGACCTCAGGTTCATCAATCTCTATTCTTATACTACTCTATAAAGAATCT
CTATATTCAAGTTTGGTTGGATGTTTTGTAAGTACAGAAAACTATTGGGT
GGGCGGGGCAGGGGGAGGAAGGAGAGAAAGAGGAGAGGGAGAGAAGGAG
(2) INFORMATION FOR SEQ ID NO: 149:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 28 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iY) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANIS~: Bos taurus (vii) IMMEDIATE SOURCE:
(B)!CLONE: MGTG3 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO~ 149:
GTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 150:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 58 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO

W 0 92/131~ 2 1 0 0 ~ 8 ~ PCT/US92/00340 f~

-la~-(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE: -(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE: }
(B) CLONE: MGTG3 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 150:
GATATACCAATGAATATCAAGAAGTTTACTACAGCCAACTGTTCCATTAA
GGTTGAAA
(2) INFORMATION FOR SEQ ID NO: 151:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 199 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOUROE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG4B (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 151:
TTCCAGTATTCTTGCCTGGGAAATCCCATGGATAGAGGAGCCTGGTGGGC
TACAGTTCATGGGGTCAGAAAGAATCAGACATGACTGAGCACACATGATG
CATTAACTTGCTGCTACAAACTCTTAAGAGCAGCTTCTTTCTTTCTCATC
TTTCTTCTGCTCTCCAGTCTCAACATCCTTGTTTCTTAGGAGGAAGTGA
~2) INFORMATION FOR SEQ IV NO: 152:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG4B (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 152:

.~ 92/1310~ 0 -3 ~ 3 PCT/US92/0034o --1~3g_ TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
~2) INFORMATION FOR SEQ ID NO: 153:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 134 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: MGTG4B (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 153:
AAATGCTTGAATCTTATAGTATCCTTTATTAATATTCTGAATAATTTGAG
GGTCGATAAACTTTTGGATGTGAGCGGAT...AAGCCCACTTAGATGTGA
TTTGTGATGCTCTTGGAAGCTTATTGTATAAAGT
~2) INFORMATION FOR SEQ ID NO: 154:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 80 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: MGTG7 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 154:
GTTCATTGCAGCACTATTTACAATAGCCAAGATATGGAAGTAACCTAAAT
GTCCATCAACAGAAGAATGAACAAAGAAGA
(2) INFORMATION FOR SEQ ID NO: 155:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 72 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: llnea W O 92/1310~ 210 0 ~ 8 3 PCT/US92/0034~ :

-19~-(ii) MOLECULE TYPE: DNA ~genomuc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG7 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 155:
TGTGGTGTGTGTGTGTATATCTATTTACACACACACACACACACACACAC
ACACACACACACACACACACAC
~2) INFORMATION FOR SEQ ID NO: 156:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 199 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: MGTG7 ( down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 156:
ACCGTAAGTTTGTTTTC~AAGTCTGTTAGTCTCTATTTTGTAAATAAGTT
CCCTGTATCATTTTTTGAAGATTCCACTTATGATATGATATTTGTTTTTC
TCTGTCTGATTTATTTCACTTTGTATGATAATCTCTAGGTCCATCCATCT
GGCTGCAAATAACATTATATTGTTCTTTTTTATGGCTGAGTAGTATCTG
(2) INFORMATION FOR SEQ ID NO: 157:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 113 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
¦ (iv) ANTI-SENSE: NO

, (vi) ORIGINAL SOURCE:
! (A) ORGANISM: Bos taurus I (vii) IMMEDIATE SOURCE:

, '~O 92/~310~ - PCT/US92/00340 2100~

-l91-(B) CLONE: TGLA10 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 157:
CCAGCTACCTCTTCCTCTGAGCATTTCTTGATTCCATGTTTGGTCAAATA
GGGGTTTGATTTGTCACAGTCTAAATTTATCCACTGTGGCTCTTACAAAT
TATTCAAATTATA
(2) INFORMATION FOR SEQ ID NO: 158:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs tB) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA10 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 158:
TGTGTGTGTGTGTGTGTGTGTATAGACAT&CATGTATATGAACATATATA
TATATA
(2~ TNFORMATION FOR SEQ ID NO: 159:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 76 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGA`NISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA10 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 159:
TAACACATCTTTCCTTGGAGACACAATGCAATCTGCAGTAGCATACATCC
TTGTTTATTTTTACATAGTACAAATG

(2) INFORMATI~N FOR SEQ ID NO: 160:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs W O 92/13102 2 1 0 0 S 8 3 PCT/USg2~00340 .~-I g (~) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii~ HYPOTHETICAL: NO `, ~iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B~ CLONE: TGLA102 ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 160:
GGTTCTTTATCACTAGCGCCACCTGGGAANCCCCCTCTCCTCCCTATACT
GGTCTATATTTCTCAGTCACCAACTATATCACAC
~2) INFORMATION FOR SEQ ID NO: 161:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
: ~B) CLONE: TGLA102 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 161:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 162:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 79 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NQ
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus - .
YO 92/1310~ ` 2 1 ~ O ~ ~ ~ PCT/US92/00340 -1~3-(vii) IMMEDIATE SOURCE:
(~) CLONE: TGLAl02 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 162:
GTGACTACCTACCCTGTGCCAAACATTGGCAGCTAAGATGCACTGTTTCC
CTCCAAGAAGGCAGGCAAGGGGAAGGAAA
~2~ INFORMATION FOR SEQ ID NO: 163:
(i) SEQUENCE C~RACTERISTICS:
(A) LENGTH: 88 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA t~enomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: ~os taurus ~vii) IMMEDIATE SOURCE:
~) CLONE: TGLAl09 ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 163:
AAAAGTTCATTGAGTAAATACTCTGTCAAGCACTTGGGATGTAGCAATCA
GTAAGAAGGACTTGTTTCCTGCTCTTCTAAAGTTTGTA
(2) INFORMATION FOR SEQ ID NO: 164:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
¦ (vi) ORIGINAL SOURCE:
(A) ORGANISM: Dos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl09 kepeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 164:
~ TGTGGTGTGTGTGGTGTGTGT

¦ (2) INFORMATION FOR SEQ ID NO: l65:
¦ ~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 78 base pairs (~) TYPE: nucleic ac~d W O 92/131~ 2 1 0 0 5 ~ 3 PCT/VS92/oO340 , --I 9~-- (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
tB~ CLONE: TGLA109 (down) ~xi) S~QUENCE DESCRIPTION: SEQ ID NO: 165:
AAAAAATCCCATGGACNGAGGAGCCTGTTGGGCTACAGCCTGTGGGGTTG
CAAAGAGTCGGACATGACTGAGCAACTA
(2) INFORMATION FOR SEQ ID NO: 166:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 108 base pairs (B) TYPE: nucleic acid tC) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) I~MEDIATE SOURCE:
(B) CLONE: TGLA110 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 166:
- AGAGTTGGACGTGACTGACCAACTAAGCACAGCACAGCATTTACTTATAC
ACACTCACCTGCATAAATTTTAAATGGATAGTAAATAAAGTTCTGAATCC
AATTCCTC
(2) INFORMATION FOR SEQ ID NO: 167:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~'O 92/13102 . PCT/US92/00340 21~03~3 -19 5 _ (vii) IMMEDIATE SOURCE:
(B) CLONE~ TGLA110 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 167:
TGTGTGTGTGTGTGTGTGTGTGT
~2) INFORMATION FOR SEQ ID NO: 168:
(i) ~EQUENCE CHARACTERISTICS:
(A) LENGTH: 135 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
t8) CLONE: TGLA110 ~down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 168:
AGTCTTGTGGGACTGGGCCCTTTACCTGTGGCATTTAATGCTATCTCCAG
GAAGACAATGTCAGAAATGAGTTGTATTTTCAGACAGCCCGCTGGTGTCA
GAGAACTTCTTGTTGTTGTGGTGGTGAAACCTAGA
~2) INFORMATION FOR SEQ ID NO: 169:
~i~ SEQUENCE CHARACTERISTICS:
~A) LENGTH: 51 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus - ~ii) IMMEDIATE SOURCE:
~B) CLONE: TGLA111 ~u~) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 169:
ATTCCGTGGACTATATTCCATGGGGTCACAAAGAGTCAGACACAGCTGAG
C

~2) INFORMATION FOR SEQ ID NO: 17C:
~i) SEQUENCE CHARACTERISTICS:

wo 92~l3~n2 ~ 1 0 0 ~ ~ 3 PCT/~S92/0034~ ~~
.

_l9~,_ (A) LENGTH: 32 base pairs (B~ TYPE: nucleic acid (C) STRANDEDNESS: si~gle (D) TOPOLOGY: linear - -~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAlll (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 170:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 171:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 54 base pairq ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TO~OLOGY: linear ~: (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
( iY) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA111 ~down) ~xi~ SEQUENCE DESCRIPTION: SEQ ID NO: 171:

ATACAATTCAATTATGTAAAAATTTGAAAAATGTTCTACTAGTAACTGAC
ACTG
t2) INFORMATION FOR SEQ ID NO: 172:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 158 base pairs (B) TYPE: nuclsic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus 'O 92/1310' PCrtUS92/0034~
210~3 _19q_ (vii) IMM¢DIATE SOURCE:
(B) CLONE: TGLA112 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 172:
CCTTGCTTACCTCTGGTACCTTCTACGGGGAGAAGGCAGAGGAGTTTGCA
TAGCTCAAAGCTAGTGGCCTTTACCAGCAAATGTCTGTGACCGGGAGCTT
TCCCTATCTCCCCCACCACTCCCCCCATTGCTAGAACCCAGGGTAGGCTG
CCTGG...
(2~ INFORMATION FOR SEQ ID NO: 173:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGT~: 37 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: line~r (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA112 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 173:
GTGTGTGTGTGTGTGTGCGTGTGTATGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 174:
(i) SEQUENCE CHAP~CTERISTICS:
(A) LENGTH: 137 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA112 (dowr.) (xi) SEQUENCE DESCRIPTION: SE~ I3 NO: 174:
TTTCTTTATGGTCCAAATCTCACATCCATACATGACTACTGG~AAAACCA

TAGCTTTGACTAGATGGACCTTTGTTGGCAAAGTAATGTCT---'GCTmTm--AACATGCTGTCTAGGTTGGTCATAGTTTTCTTC-...
(2) INFORMATION FOR SEQ ID NO: 17~:

W O 92tl3102 2 1 0 0 ~ 8 3 PCTlUSg2/00340 {---198-- ~
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 base pairs (~) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA116 (up) txi) SEQUENCE DESCRIPTION: SE2 ID NO: 175:
AAAAATAGCACAGTAATAAGAGTGATGGCAGAA
~2) INFORMATION FOR SEQ ID NO: 176:
~i) SEQUENCE CHARACTERISTICS:
(A) L~NGTH: 20 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOT~ETICAL: NO
¦ (iv) ANTI-SENSE: NO
¦: (vi) ORIGINAL SOURCE:
¦ (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA116 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 176:
GTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 177:
~i) SEQU~N OE CHARACTERISTICS:
(A) LENGTH: 68 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO

~iv) ANTI-SENSE: NO
~i) ORIGINAL SOURCE:

.

~0 92/13102 PCT/uS92/00340 21~0~

_,99 (A) ORGANISM: Bos t~urus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAll6 (down) (xi) SEQUENCE DXSCRIPTION: SEQ ID NO: 177:
TTTCTGTAGCAGCTCCCTATTTTCCCCAGTTTGGAGAAGATTTGGCTGTG
TACCCAGTTTAAAGTGAA
(2) INFORMATION FOR SEQ ID NO: 178:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 48 bzse pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: siAgle (D) TOPOLOGY: Ii~ear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAT: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAll7 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 178:
GTTTGTAATGCTTTCTCTGCGATTATCCATTGGTTACCACAGCCAGCT
~2) INFORMATION FOR SEQ ID NO: l79:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
tA) ORGANISM: 30s taurus (vii) IMMEDIATE SOURCE:
(3) CLONE: TGLAl17 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 179:
TGTGTGTGTGTGTGTGTGTGTG

~2) INFORMATION FOR SEQ ID NO: l80:
(i) SEQUENCE CHARACTER'STICS:
(A) LENGTH: 29 base pairs W O 92/13~0~ 2 1 0 0 S 8 3 PCT/~S92/00340 f--2~-(B) TYPE: nucleic acid (C) STRANDEDNESS: si~gle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTEIETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA117 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 180:
GATCTAATTTAAGTGTGCGTCCATGCATG
(2) INFORMATION FOR SEQ D NO: 181:
(i) SEQUENCE CHARACTERISTICS:
~A) 1ENGTH: 77 base pairs (~) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA12 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 181:
AGTGTGTGATGAAAACATTATTTGTTGCTATTTGAAATAGTTATCAAATT
TGGTTCCAATAATAATATGTTTGTGCA
(2) INFORMATIO,N FOR SEQ ID NO: 182:
(i! SEQUENCE CHARACTERISTICS:
(A) LENGTH: 27 base pairs I (B) TYPE: nucleic acid ! (c) STRANDEDNESS: single I (D) TOPO~OGY: linear ¦ (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 3Os taurus `~0 92/1310~ PCT/US92/0034~
210~a~3 --20( -~vii~ IMMEDIATE SOURCE:
(B) CLONE: TGLAl2 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 182:
TGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFO~MATION FOR SEQ ID NO: 183:
(i) SEQUENCE CHARACT3RISTICS:
(A) LENGTH: 88 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANIS~: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLQNE: TGLAl2 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 183:
TTCCCAGAGGTTGAGGTTCTGAGGAACTTGGGGCCTGAAGAGGTTTCTGG
GTGCCACACCATAGCATCTACTACATGCATGTTTGTGT
(2) INFORMATION FOR SEQ ID NO: 184:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl22 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1~4:

¦ TCCAGGTAAATCAGC
, ~2) INFORMATION FOR SEQ ID NO: 185:

¦ (i) SFQUENCE CHARACTERISTICS:
(A) LENGTH: 4l base pai-s (3) TYPE: nucleic acie W O 92/13102 2 1 0 0 ~ 8 ~ PCT/US92/~034n ~ --(C~ STRANDEDNESS: single (D1 TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) tiii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos tauru~
(vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA122 (repeat) (xi) S~QUENCE DESCRIPTION: SEQ ID NO: 185:
ATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: l86:
(i) SEQUENCE CHARACTE~ISTICS:
(A) LENGT~: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MCLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(8) CLONE: TGLAl22 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 186:
AAACGACAACAATAACCAGAAAACAATCACATGGCAAATAAGTACATACC
TATGAATAATTTT
(2) INFORMATIQN FOR SEQ ID NO: 187:
(i) SEOUENCE CHARACTERISTICS:
(A) LENGTH: 6~ base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURC-:

YO 92/~310~ PCT/~S92/00340 2100~

(B) GLONE: TGLA123 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 187:
GATCAGGGCTCAGACTGCTACTAGACAAAGAAGAGAAGCCAAGCTTTGCT
TCTGCTTCTCCACAGGCCC
~2~ INFORMATIQN FOR SEQ ID NO: 188:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 26 base pairs (~) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE-(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA123 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 188:
GTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 189:
ti) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 55 base pairs I ~B) TYP~: nucleic acid I ~C) STRANDEDNESS: single tD) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) ¦ tiii) HYPOTHETICAL: NO
¦ tiv) ANTI-SENSE: NO
tvi) ORIGINAL SOURCE:
, tA) ORGANISM: 8cs taurus ¦ ~vii) IMMEDIATE SOURCE:
¦ tB) CLONE: TGLA123 tdown) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 189:
GCCTGCCTGCTGGTGAGGTCTGGCTCTGGGCCCTGGGCTGAGGGAGGATG
AACAG
t2) INFORMATION FOR SEQ ID NO: l90:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 60 base pairs t8) TYPE: nucleic acid tC) STRANDEDNESS: si~gle ., Y40 92/1310' 2 ~ O 0 5 8 3 PCT/US92/0034~ t `

(D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO '.
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TG~Al24 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: l90:
GATCTAGGGAGTTGGGATGATGTGGAAGCAAGAGATGGAAAAAGAGGACC
AGTGAATGCA
(2) INFORMATION FOR SEQ ID NO: 191:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 29 base pairs (B) TYPE: ~ucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MO~ECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl24 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: l9l:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGT
~2) INFORMATION FOR SEQ ID NO: 192:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 59 base pairs (B) TYPE: nucleic ac'd (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos tau-us (vii) IMMEDIATE SOURCE:
(B) CLONE: TGEAl24 (down) ~0 92/13102 PCT/US92/0034n ~1~0~8~

(xi) SEQUENCE D~SCRIPTION: SEQ ID NO: l92:
AATTCTTAGTCTAGTTCAGGTAGGCTTTTATTTCTGTCTCTTCCTGTATA
GGCCTTTAT
(2) INFORMATION FOR SEQ ID NO: l93:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl25 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 193:
GCTGTGTTTTCCTACAGTCCTCATACTATTTCTTTCGATTTCCCCATGAT
AAACAG
(2) INFORMATION FOR SEQ ID NO: 194:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 base pairs (B) TYPE: nuclelc acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(Yi ) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl25 (repeat) - (xi) SEQU~NCE DESCRIPTION: SEQ ID NO: 194:
AMAZING
(2) INFORMATION FOR SEQ ID NO: lg_:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 34 base pairs (B) TYPE: nucleic acid ', (C) STRANDEDNESS: single (3) TOPOLOG~: linea-W O 92/13]02 21 0 0 ~ 8 3 PCT~US92/00340 !

- ~2 ~ -(ii) MOLECULE TYPE: DNA (g~nomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOUROE :
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA125 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 195:
GAGCTGGCCTTGTTGAGACCTTCTGTGTGCATGT
(2) INFO~MATION FOR SEQ ID NO: 196:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 59 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA126 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 196:
TTTATATTTAGGAACTTTCCACTACTCTCTGCTAATTTAGAATGAGAGAG
GCTTCTGGA
~2) INFORMATION FOR SEQ ID NO: 197:
(i) SEQUENCE CHARACTERISTICS:
(A? LENGTH: 34 base pairs tB) TYPE: nucleic acid (C) STRANDEDNESS: single (D~ TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA126 (repeat) ~O 92/1310~ PCT/~S9~/00~4~

(xi) SrQUENCE DESCRIPTION: SEQ ID NO 197: .
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 198: r (i) S~2UEN OE CHARACTERISTICS: ~r (A) LENGTH: 81 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOUROE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl26 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 198:
TTGGAGTACTGTAAAGGAATTAAAGGACCTTGGCTCTATTCTCTGAATAT
TCCCCAC~ACACCCACACCCATCTCCCCCCT
(2) INFORMATION FOR SEQ ID NO: l99:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 104 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY:. linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOUROE:
(B) CLONE: TGLAl27 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: l99:
TGCAATTGTGTGGTAGTTTGGACATTCTTTGGCATTGCTTTCTTTGGGAT
TGGAATTAAAACTGACTTTTTCTAGTCCTGTGGCCACTGCTGAGTGGTTT
GATG
(2) INFORMATION FOR SEQ ID NO: 200:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 35 base pai~s (B) TYPE: nucleic acid (C) STRANDEDNESS: sinsle (D) TOPOLOGY: linea-W 0 92/13107 210 0 5 ~ 3 pcT/uss2/oo34n f - 20~-(ii) MOLEOULE T~PE: DN~ (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl27 Irepeat) (xi) SE2UENCE DESCRIPTION: SEQ ID NO: 200:
ATATATGTGTGCGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 201:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl27 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 201:
ACAGAGAAGATATAATTAACATGTATTGATTAGACCAAGACACTATTGCA
AAGGACCTCCAATT
(2) INFORMATION FOR SEQ ID NO: 202:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 71 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA;28 (u~) ` YO 9~/1310~ PCT/~IS92/00340 2101)~

(xi) SEQUENCr DESCRIPTION SEQ ID NO: 202:
TGAAACTATAGTTGATTTACAATGTTTTTTTAGTTTTACATGAACAGCAA
AG~GAGTCAGTTATGTAGACA
- (2~ INFORMATION FOR SEQ ID NO: 203 ~i) SEQUENCE CHARACTERISTICS
(A) LENGTH 37 base pairs ~B) TYPE: nucleic acid (Cj STRANDEDNESS single ~D) TOPOLOGY: linear ~ii) MOLECU~E TYPE: DNA (genomic) ~iii) HYPOTHETICAL NO
~iv) ANTI- SENSE: NO
(vi) ORIGINAL SOURCE
(A) ORGANISM Bos taurus (vii) IMMED IATE SOURCE:
(B) CLONE TGLAl28 (repeat) (xi) SEQUENCE DESCRIPTION SEQ ID NO: 203 TGTGTGTGTGTCTATGTGTGTGTGTGTGTGTGTATGT
(2) INFORMATION FOR SEQ ID NO: 204:
(i) SEQUENCE CHARACTERISTICS
(A) LENGTH 12 8 base pairs (B) TYPE nucleic acid (C) STRANDEDNES S: single ~D) TOPOLOGY; linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL NO
(iv) ANTI-SENSE NO
(vi) ORIGINAL SOURCE
(A) ORGANISM Bos taurus (vii) IMMEDIATE SOURCE
~B) CLONE TG~A12 8 (down) (xi) SEQUENCE DESCRIPTION SEQ ID NO: 204:
TGCACACATCAGGCCTTCTGGGAGCATGGACACATGTGTGGAGGAAGGAG
GGAATTTCAAAGGCAAAGCAACTGTTTCCAACCAGAGACAATGAATTCAA
ATAAATACAATAATTAATTATATCAAGA
(2) INFORMATION FOR SEQ ID NO: 205 (i) SEQUENCE CHARACTERISTICS
~A) LENGTH 88 base pa~rs (B) TYPE nucleic acid (C) STRANDEDNESS s~ nsie (D) TOPOLOGY llnear WO 92/13102 210 0 3 8 3 PCr/US92/0034û

(ii~ MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA13 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 205:
CTGTGTTCTCTATTGTGTTCCATTGGTATATTCCAGAGAATGTTTAAGAG
CCTCTTTTTTACTTGTGCTCCATATGTATATAAAACAT
(2) INFORMATION FOR SEQ ID NO: 206:
(i) SEQUENCE CHARACTERISTICS:
(A~ LENGTH: 63 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~: tiii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~- (vi) ORIGINAL SOURCE:
~ (A) ORGANISM: ~os taurus -~ ; (vii) IMMEDIATE SOURCE:
: (B) CLONE: TGLA13 (repeat) ~xi) SEQUENCE DESCRIP~ION: SEQ ID NO: 206:
ACACATGTGTGTGTGTGTGTGTGTATATATATATATATATATA-ATATAA
CATTATATATATA
(2) INFORMATION FOR SEQ ID NO: 207:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 152 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANIS~ os taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA'3 (down) ~O 92/1310~ 21~ O ~ ~ 3 pcT~uss2/oo34n (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 207:
GTACCAGAAGAGACTAGAGAGGAAATGTGCAGAAGAACTATTTGAAGAAA
TAATGGCTCATTTTTTTTTTTTCTGAAATAGATAAAAGACAGCAATGCAG
AAATCCACAAAGCTCATCAAACATTTAATATACATATATGACTATGTGCC
CC
~2) INFORMATION FOR SEQ ID NO: 208:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: l0l base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus -~ ; (vii~ IMMEDIATE SOURCE:
(B) CLONE: TGLAl30 (up) xi) SEQUENCE DESCRIPTION: SEQ ID NO: 208:
CCAACTGGCCAGTCATAATAAATTGACACTGATTC~TTTTTCATCTGAAA
ACAATAAAATAAAATAACAATACATATAGAGGATGATACCAT-TATGT..

(2) INFORMATION FOR SEQ ID NO: 209:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 26 base pairs : (B) TYPE: nucleic acid (C) STRANDEDNESS: single `~ (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~:- (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~ (B) CLONE: TGLA130 (repeat) ¦~ (xi) SEQVENCE DESCRIPTION: SEQ ID NO: 209:
GTGTGTGTGTGTGTGTGTGTGTGTGT

. (2) INFORMATION FOR SEQ ID NO: 210:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 4l base pairs I

W O g2/1310' 21 0 0 5 8 3 pcT/us92/oo34n f - ~12^
(B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv~ ~NTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA130 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 210:
ATGGGGCCGCAAAGGGTTGGATGCACTGTGCATGTGTGCAA
(2) INFOR~ATION FOR SEQ ID NO: 211:
(i) SEQUENCE C~ARACTERISTICS:
(A) LENGTH: 181 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single : (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) tiii) HYPOTHETICAL: NO
(iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
: (A) ORGANISM:. Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA131 (up) ~:; (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 211:
:~ TGGTAACTCTGAATTTCAGTTGGTTTAGATTCCACATATAAGTGATATCA
TACAGTATTTGTCTTTCTCTAACTTATTTCACTTAGCATAATGCCCTCAG
GGTTCATCCATGTTGTCAGAAATGGAAGAACACCCATCTTTTTTCATGGC
TGAGTAATATTCATGTTTTTGTATCTGTGTC
~2) INFORMATION FOR SEQ ID NO: 212:
SEQUENCE CHARACTERISTICS:
(A) LENGT~: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single : (D) TOPOLOGY: linea~
(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NC
(vi) ORIGINAL SOU~CE:

.. . . ... .. . .. . . . . . .

~O 92/13102 2 1 Q 0 5 ~ ~ PCT/US92/0034n _ ~l3 ~
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
tB) CLONE: TGLAl31 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 212:
TGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 213:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 34 base pairs :~ ~B) TYPE: nucleic acid : IC) STRANDEDNESS: single (D) TOPOLOGY: linear ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
: (vi) ORIGINAL SOURCE:
A) ORGANISM: Bos taurus vii) IMMEDIATE SOURCE:
B): CLONE: TGLAl31 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 213:
GATCAATGGATAAAGAAGATGTGTACACATACGT
(2) INFORMATION FOR SEQ ID NO: 214:
(i) SEQUENCE~CHARACTERISTICS:
(A) LEMGTH: 65 base pairs (B) TYPE: nucleic acid :(C) STRANDEDNESS: single D) TOPOLOGY: linear ~ (iij MOLECULE TYPE: DNA (genomic?
--~ (iii) HYPOTHETICAL: NO
, ! (iV) ANTI~SENSE: NO
(vi) ORIGINAL SOURCE:
A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~:~: (B~ CLONE: TGLAl32 (up) I -:
-. (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 214:
~:~ TTGCAAATACTGTGAAATGGTACCTTTTTAAACCCTTGGTTTCTAATTGT
TTATTGCTAGTGTAA

(2) INFORMATION FOR SEQ ID NO: 2'_:
~: (i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 37 base pairs W O 92/1310~ 2 1 0 0 5 ~ 3 PCT/US92/00340 : ~

(B) TYPE: nucleic aGid (C) STRANDEDNESS: single (D) TOPOLO&Y: linear (ii) MOLECULF TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(3) CLONE: TGLAl~2 (repeat) ~xi~ SEQUENCE DESCRIPTION: SEQ ID NO: 215:
TGTGTGTGTGTGTGTGTGTGTGTGTGTATATGTATAT
(2) INFORMATION FOR SEQ ID NO: 216:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 92 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: N0 ~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM:~ Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl32 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID N0: 216:
AGTTTACCAAGGTTGCAGGATATAAGTCAATATAAATCAATCATATTTAT
; ATACACTTGTTGTTCAGTTGCTAAGTCATGTCTTTCTCTTTT
(2) INFORMATION FOR SEQ ID NO: 217:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 68 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sin~le (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: N0 (i~) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus -~0 92/1310' 2 1 0 ~ 5 ~ 3 PCT/US92/0034~

(vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl34 (up) txi) SEQUENCE DESCRIPTION: SEQ ID NO: 217:
AAAACGCAAGATTCAGCGAACTTTTTCTGTAAATGAGCAGATAGTAAACA
TTTGAGTCTTGGTAGGAC
-(2) INFORMATION FOR SEQ ID NO: 218:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 35 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~: (D) TOPOLOGY: linea~
~ (ii) MOLECVLE TYPE: DNA (genomic) : (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE~
(A) ORGANISM: ~os taurus vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl34 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 218:
GTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTG
E~2) INFORMATION FOR SEQ ID NO: 219:
(i) SEQUENCE CHARACTERISTICS:
~N~:- -: (A) LENGTH: .53 base pairs ) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear MOLECULE TYPE: DNA (genomic) i) HYPOTHETI;CAL: N0 .
: (iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANIS~: aOs taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl34 (down) . ~:
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 219:
~:`

TCCAGGTGGCACACAGGGTCGCAAGAGTTGGACATGACTGAAG-GACTCA
~: GTG
(2) INFORMATION FOR SEQ ID NO: 220:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 base pairs (B) TYPE: nucleic aci~

W O 92/131~2 2 1 0 0 5 8 3 PCr/US92/0~340 --~16 -(C) STRANDEDNESS: single - ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomuc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA135 (up) (xi) :SEQUENCE DESCRIPTION: SEQ ID NO: 220:
GATCCACTCACTTTTCTGAAGAATTCCAGATAGATTTCGTCTGAAATTAA
ACACATATGTGAGCA
(2) INFORMATION FOR SEQ ID NO: 221:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~:. (D) TOPOLOGY: linear (i,i) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
1v) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus , ~: ~ (vii) IMMEDIATE SOURCE:
(B) CLONE: TG~A135 (repeat) .(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22l:
~: TGTGTGTGTGTGTGTGTGAGTG
-~ (2) INFORMATION FOR SEQ ID NO: 222:
(i) SEQUENCE CH M ACTERISTICS:
: (A) LENGTH: 29 base pairs (B) TYPE: nucleic acid ~:~ (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~: (ii) MOLECULE TYPE: DNA ~genomic) , -~ : (iii) HYPOTHETICAl NO

- ~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMNEDIATE SOURCE:

.. . . . ~ . . . . . . . . . . .

~O 92/1310~ . PCT/US9?/00340 - 2 1 ~

- (B) CLONE: TGLAl35 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 222:
AAAGAGTTGGACATGACTGAGTGACTAAA
(2) INFORMATION FOR SEQ ID NO: 223:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (Yii ) IMMEDIATE SOURCE:
(B) CLONE: TGLAl37 (up) : (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 223:
AACTTTTCAGATTATCCTACTACTTAAGGGGGTTGACTTGTTAATCACTG
ACAGCCCATTTGA
(2) INFORMATION FOR SEQ ID NO: 224:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 29 b~se pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOT~ETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~ii) IMMEDIATE SOURCE:
~B) CLONE: TGLA137 ~repeat) Ixi) SEQUENCE DESCRIPTION: SEQ ID NO: 224:
TGTGTGTGTGTGTGTGTTGTACCTGTGTG
(23 INFORMATION FOR SEQ ID NO: 225:
(i) SEQUENC~ CHARACTERISTICS:
(A) LENGTH: 9~ base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linea W O 92/1310? `~ l a 3 ~ PCT/USg2/00340 t-"

(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLOWE: TGLA137 (down) (xi) SEQUENCE DESCRIP~ION: SEQ ID NO: 225:
AAACAACTGCCTGCAGGATTGGTTGTCACAGAGAAATTGTAGCTCTCCCT
TAGACACACGTGAAGTCCACCTGTGCATCATTCATTGCACATAT
(2) INFORMATION FOR SEQ ID NO: 226:
(i) SEQUENCE CHARACTERI$TICS:
(A) LENGTH: 72 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
B) CLONE: TGLA141 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 226:
. ACTGACTTGCACAGACAGCTGAGAAGAGTCTAGTCCCATCATTGTGGTAG
ACATTTGAACTTGGTCCTAGGA
(2) INFORMATION FOR SEQ ID NO: 227:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 18 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) i) HYPOTHETICAL: NO
(i~) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos ;aurus ~vii) IMME3IAT_ SOURCE:
~B) CLONE: TGLA1~1 (repeat~

VO 92/1310~ . PCT/US92/0034~

21005~
-2~9-~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 227:
GTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 228:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 83 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sinsle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
B ) CLONE: TGLAl4l ~down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 228:
~: AAAGCTGGCCCAGCTGTACTTTGTTTTATCTCCATAATCTTCACTCCAAA
CCCCGCTTCTAGTTGGGGCCCTCCCTCCTAAAT
; ~2) INFORMATION FOR SEQ ID NO: 229:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 78 base pairs ~B) TYPE: nucleic acid (C) STR~NDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~; ~B) CLONE: TGLAl42 ~up) : ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 229:
ACTTCAGTCTCTTTGCCAAGAGGGAATGAATGGAGTGTTTCTTTTCGCCC
TCTATATGTTGTTTTAGCTGGATTTTAT
(2~ INFORMATION FOR SEQ ID NO: 230:
~i) SEQUENCE CHARACTERISTICS:
~A~ LENGT~: 48 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linea-W 0 92tl3~02 PCT/uS92/0034n ~~

~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA142 kepeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 230:
TTGTGGCTGTAGAGTGAGTGTGTGTGTGTGTGAGTGTGTGTGTGATG~
(2) INFORMATION FOR SEQ ID NO: 231:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 67 base pairs ~B) TYYE: ~ucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAh: NO
~iv1 ANTI-SENSE: NO
-~ (vi~ ORIGINAL SOURCE:
A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA142 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 231:
AAAGATGTCAGGACAACAACGGAAGTATTAGGACCATGAAGAGCAGTCAT
~- GCTGAATCAATTACTGA
(2) INFORMATION FOR SEQ ID NO: 232:
(i) S~QUENCE CHARACTERISTICS:
: ~A)jLENGTH: 45 base pairs : ' ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl4, (up) ~ ........ . .... . . . . . . . . . .

YO 92/131~ PCT/Us92/00340 2 1 ~ 0 5 8 ~3 _ ~ 2l-(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 232:
TTCTCTTGAGAAGAACAGAGTGCTATGCATTTAACCAAGGATGTA
(2) INFORMATION FOR SEQ ID NO: 233:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l9 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl47 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 233:
. TGTGTGTGTGTGTGTGTGT
~2) INFORMATION FOR SEQ ID NO: 234:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 70 base pairs - (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear : (ii) MOLECULE TYPE: DNA (genomic) ~ (iii) HYPOTHETICAL: NO
:~ (iv) ANTI-SENSE: NO
:~ (vi) ORIGINAL SOURCE:
: (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
! I " ~ ( B) CLONE: TGLAl47 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 234:
AATATTTTAAAATAGAACCATCCTGGAAAAATGCAAAATAGGAAATTTAG
ACACACAAACATACCCAAGA
(2) INFORMATION FOR SEQ ID NO: 235:
(i) SEQUENCE C~ARACTERISTICS:
~A) LENGT~: 89 base pairs (~) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) W O 92/l310~ 2 1 0 0 ~ ~ 3 PCT/US92/00340 ~

~iii) HYPOTHETICAL: NO
tiV) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~ ;
(vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA149 tup) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 235:
TTTTTTTAATTGAAGTATAATTGACATATATTATATTACTTCTTCAATAT
CTTGTAATAACTTATAATGGAAAAAAATCTGAAAAGCAA
~2) INFORMATIQN FOR SEQ ID NO: 236:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
vi) ORIGINAL SOURCE:
: ~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
B) CLONE: TGLA149 (repeat) . .. . .
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 236:
TATATATGTGTGTGTGTGTGTGTGTGTGTGTGT
~ (2) INFORMATION FOR SEQ ID NO: 237:
:~: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 81 base pairs (B) TYPE: nucleic acid , ~ , (C~STRANDEDNE$S:,single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~ ~:
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA149 (down) (xi) SEQUENCE DESCRIBTION: SEQ ID N^: 23.~:

` 'O 92/13102 PCT/US92/00340 ~100~3 CTCAATTGAAGTGCACTTGATTTACAATGTTTCAGGTATATCACAAAG~G
ATTCAGTTTTATATATGTGTATGTGTTTGAG
(2~ I~FORMATION FOR SEQ ID NO: 238:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 198 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl5 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 238:
: CCA~TCAGTAGAAGTTACTTCATGGAATGAAGGATATGTTGAAATAAGAC
AGAAGTTGGTGACTGTACAACACTGTGAATGAAC$AATGGCACAAAATTA
TTTGCTTTAAATGGCATCTGAATATATATACATATGAATTATATTTTAAT
ATATAATATTCTGTATAATGCATAATATAATCAAATGATTGATTCCAC
(2) INFORMATION FOR SEQ ID NO: 239:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid ~: (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 30s ta~rus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl5 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 239:
TGTGTGTGTGTGTGTGTGTGTGTGTGGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 240:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 94 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linea-W O 92~1310~ 21 0 0 ~ 8 3 PCT/US92/00340 . - 2 - (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLON~: TGLA15 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 240:
GCATGAGAAACCACACGGACAGAGGAGCCTGGTAGGCTATGGTCCAAGGG
TTCACAAAGAGTTGGACATGACTGAGCGAATAAACTACAACAGT
(2) INFORMATION FOR SEQ ID NO: 241:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 97 base pairs (B) TYPE: ~ucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
- (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~-~ (vii) IMMEDIATE SGURCE:
; (B) CLONE: TGLA153 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 241:
: CTGATGTATAGCACTGGGAGCTCAGCTAGGTTCTCTGTGATGACCTAGAA
GGGTTTGGTGGGTGAGGGAGTGGGAGAAAGGCTCAAATAGGAGAGGA
~2) INFORMATION FOR SEQ ID NO: 242:
(i) SEQUENCE CHARACTERISTICS:
! (A) LENGTH: 51 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: ~in~le (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: N0 (iv) ANTI-SENSE: N0 (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA153 ~repe~t~

- ~O 92/1310~ PCT/US92/00340 210~a83 ~22~-(xi) SEQUENC~ DESCRIPTION: SEQ ID N0: 242:
TATATGTATATGTATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG

~2) INFORMATION FOR SEQ ID NO: 243:
~i~ SEQUENCE CHARACTERISTICS:
(A) LENGTH: 59 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D~ TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl53 (down) (xi) SEQUENOE DESCRIPTION: SEQ ID NO: 243:
- AATGCAATTGCTTTACAGTGTTGTGTTAGTTTCTGTTGTACAACTGCATG
ATATAGCAT
2) INFO~MATION FOR SEQ ID N0: 244:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 79 base pairs : ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
:~ (iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(Aj ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~: (B) CLONE: TGLAl54 (up~
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 244:
TTAGCGTGGTTGAATGAAGCTCTTTGCTAAGACCAATATAGAGAATTATG
TTGTCAGATACTTCAGCTTGGAATGGGGA
~2) INFORMATION FOR SEQ ID NO: 245:

(i) SEQUENCE CHARACTERISTICS:
(A~ LENGTH: 27 base palrs ~B) Typr: nucleic acid (C) STRANDEDNESS: sing}e WO 92/13102 2 1 0 0 ~ 8 3 PCl`/US92/00340 1~

- 22l~ -(D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) tiii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos tau~us ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl54 ~repeat) (xi) SEQUENCE DESCRIPT~ON: SEQ ID NO: 245.
TGTGTGTGTGTGTGTGTATATGTGTGG
(2) INFORMATION FOR SEQ ID NO: 246:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 131 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOT~ÆTICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~-:(vii) IMMEDIATE SOURCE:
¦~(B) CLONE: TGLA154 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 246:
AAAAATGAAGTAGGTTCCCCAGGACCATATCAACAGAAACCTAAATTCCA
ACTGGACCTGGTACATAAAGACTCCAACATTACGTGATTATGA_ACTGAT
ATAAAAGTTTGGTTTTCTCAACTCCCTGGGC
(2) INFORMATION FOR SEQ ID NO: 247:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 66 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single I(D) TOPOLOGY: linear ¦(ii) MOLECULE TYPE: DNA (genomic) ¦(iii) HYPOT~ETICAL: NO
¦~iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:

'O 92~13102 PCT/~S92/00340 2 1 ~ O ~ ~ 3 (B) CLONE: TGLAl58 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 247:
GGTTTTGTCAGAAAAATGGAAAGATTTACTGGATGATGGTCTCAATTTGT
AAAGTGCACTGTAGTA
(2) INFO~MATION FOR SEQ ID NO: 248:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l6 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ; (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vij ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
: (B) CLONE: TGLAl58 (repeat) ~ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 248:
:~: TGTGTGTGTGTGTGTG
~2) INFORMATION FOR SEQ ID NO: 249:
li) SEQUENCE CHARACTERISTICS:
A) LENGTH: 49 base pairs B) TYPE: nucleic acid (C) STRANDEDNESS: single - (D) ~OPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGiINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B~ CLONE: TGLAl5S (down) -~ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 249:
TTTTTCATACTCACACCAAGTTCACTTTTAP~CAGGTATCTTTTTmTCTT
~2) INFORMATION FOR SEQ ID NO: 250:
(i) SEQUENCE C~ARACTERISTICS:
~(A) LENGTH: 99 base pairs (B) TYPE: nucleic acid (C) STRAN3EDNESS: single (D) TOPOLOGY: linea~

W O 92/13102 PCT/USg2/00340 ~
21005~3 --2~3 _ (ii) MOLECULE TYPE: DNA (genomic) (iii) RYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
tA) ORGANISM: Bos taurus (vii~ IMMEDIATE SOURCE:
(B) CLONE: TGLAl59 ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 250:
AGAGACATACCTGTGGCATCCAGGGAACAAATTACAAACCATATTCTCAC
. CATTCTGTCTTTCCTACTCCTCCTCTCTTGGGTCTCTTGCATTATTATC
(2) INFORMATION FOR SEQ ID NO: ~51:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 44 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single . (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) iii) HYPOTHETICAL: NO
(iv~. ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
B) CLONE: TGLAlS9 (repeat~
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 251:
:~ TGTGAGTGTGTGTGTGTGTGTGCGTGTGCAAGTGCATGTGTGTG
2) INFORMATION FOR SEQ ID NO: 252:
(i) SEQUENCE CHARACTERISTICS:
~A) jLENGTH: 86 base pairs i~ (B) TY~E: nucleic acid (C) STRANDEDNESS: single ~ (D) TOPOLOGY: linear :~ tii) MOLECULE TYPE: DNA (qenomic) (iii) HYPOTHETICAL: NO
tiv) ANTI-SENSE: NO
l ~ (vi) ORIGINAL SOURCE:
¦~: (A) ORGANISM: Bos taurus ~: (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl59 (dow~) 'O 92/13102 PCT/USg2/0034n 210~3~ 3 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 252:
TTTTTATTTCGAATCTCTTGAGTACAGACA~rCTTTGCCATTCTTCCTCAG
TTCTTACTTTTATGCAAAAGCAGATTCACATAAGTG
(2~ INFORMATION FOR SEQ ID NO: 253:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 88 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl60 ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 253:
AAAATATATTGTCTTAATAGAATACGTTGTTTTGTTTTTAAAGCTCTTTG
AAGACATGAGAAGATTCCCACTTTCCACACCTGGGTCG
~:~ (2) INFORMATION FOR SEQ ID NO: 254:
~ i) SEQUENCE C~ARACTERISTICS:
:- ~A) LENGT~: 16 base pairs ) TYPE: nucleic acid C) STRANDEDNESS: single D) TOPOLOGY: linear .:
~ (ii) MOLECULE TYPE: DNA ~genomic) ,~
YPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
!~ I . (A)i ORGANISMi:~Bos taurus -~ (vii) IMMEDIATE SOURCE:
~ B) CLONE: TGLA160 ~repeat) : (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 254:
GTGTGTGTGTGTGTGT
(2) INFOR~ATION FOR SEQ ID NO: 255:

~i) SEQUENCE CHARACTERISTICS:
: ~A) LENGTH: 80 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linea-W O 92/13102 2 1 0 0 ~ 8 3 PCT/U592/oO340 1- `

- 23~ -(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE: .
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl60 (down) (xi) SEQUENCE D~SCRIPTION: SEQ ID NO: 255:
TTTCAGCAAAAGTGTCTGTGTTATGCATCATGAAGGTGGAGTGTAAGGAA
ATTCCAAAAATATTCCCCCGGCCCCATAGA
; (2j INFORMATION FOR SEQ ID NO: Z56:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 49 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA (genomic) ~ (iii) HYPOTHETICAL: NO
:~ (iv) ANTI-SENSE: NO
(vi1 ORIGINAL SOURCE:
(A~ ORGANISM: 3OS taurus (vii) IMMEDIATE SOURCE:
~ (B~ CLONE: T¢LA}62 (up) '`~-` ~ (Xl) SEQUENCE DESCRIPTION: SEQ ID NO: 256:
GGTGCCGTCTATGGGGTCGCACAGAGTTGAACACGACTGAAGCGACTTA
121 INFORMATION FOR SEQ ID NO: 257:
(i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 15 base pairs . , ~ (B~ TYPE: nucleic acid :: (C) STRANDEDNESS: single ~; (D) TOPOLOGY linear ii) MOLECULE TYPE: DNA Igenomic) (iii) HYPOTHETICAL: NO
i~) ANTI-SENSE: NO
~ vi) ORIGINAL SOURCE:
: :~ (A) ORGANISM: Bos taurus :: (vii) IMMEDIATE SOURCE:
. (B) CLONE: TGLAl62 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NC: 257:

'O 92/13~0' - PCT/US92/0034n 2 1 0 3 ~

_ 2 3~-GCAGCAGCAGCAGCA
(2) INFORMATION FOR SEQ ID NO: 258:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 36 base pairs (Bj TYPE: nucleic acid (C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomlc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA162 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 258:
GATCAGTGGTTCTCAAAGTGCCTTCACAGCATCACC
~2) INFORMATION FOR SEQ ID NO: 259:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 44 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (lii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~- (vi) ORIGINAL SOURCE:
~:~ (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B)ICLONE: TGLA164 (up) . ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 259:
TTACCAGGTAATTAACTTGATGGAACTAAAACTAAAACTGTCTT
~2) INFORMATION FOR SEQ ID NO: 260:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 58 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

W O 92~1310~ PCT/US92/0034~
2 1 0 0 ~ 8 3 - 2 3 ~ ~

tiv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl64 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 260:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTGTGTGTGTG
TGTATGTG
(2) INFORMATION FOR SEQ ID NO: 261:
(i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 80 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A~ ORGANISM: Bos taurus vii) IMMEDIATE SOURCE:
: (B) CLONE: TGLAl64 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 261:
CCCATGGATAGAGGAGCCTGGCAGGCTACGGTCTATAGGGTCACACAGAG
TCGGACATGACTGAAGCAACTTAGCATGTG
t2) INFORMATION FOR SEQ ID NO: 262:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 53 base pairs tB) TYPE: nucleic acid (C) STRANDEDNESS: sin~le (D)ITOPOLOGY: linaar (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTNETICAL: NO
(iv) ANTI-SENSE: NO
(Yi ) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus : (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl7 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 262:
TTTAACTTCTATGCAGAGTACATCATGAGAAATGCTGGACTGGAT~AAGC

/0 92/13102 PCT/uS92/003~
;, 8 ~

ACA
t2) INFORMATIOM FOR SEQ ID NO: 263:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 53 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) ~OLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl7 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 263:
TGTATATGTATGTGTGTGTGTGTCTGTGATGGTGTGTGTGTTTGTGTGTG
TGT
(2) INFORMATION FOR SEQ ID NO: 264:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 64 base pairs ~: (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
B~ CLONE: TGLAl7 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 264:
GGTTGCAAAGAACTGGACATAACTGAGTGATTGAAATGAACTGATATCTT
: GTTGCAGACTTCTT
(2) INFORMATION FOR SEQ ID NO: 265:
(i) SEQUENCE C~ARACTERISTICS:
~A) LENGTH: 48 base pairs (B) TY~E: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (geno~.ic) , .,'1 . ; . , , : . ~ , , ! , , ... . . ~ , . . . . . .

WO 92/13102 2 1 0 0 ~ 3 PCI/US92/00340 -~3~-- ;
~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos ~aurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA170 ~up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 265:
CTATGCTGTACAGTAGGTCCTTGTTGATTATGTTACACATAGTATGCA
~2) INFORMATION FOR SEQ ID NO: 266:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 34 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linea_ (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA170 ~repeat) xi) SEQUENCE DESCRIPTION: SEQ ID NO: 266:
TGTGTGTGTGCGGCGTGTGTGTGTGTGTGTGTGT
(~2): INEORMATION FOR SEQ ID NO: 267:
~-~ (i) SEQUENCE CHARACTERISTICS:
; (A) LENGTH: 52 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~: ~ (D)j TOPOLOGY,: linear i, ~
(ii) MOLECULE TYPE: DNA ~genomic) ~: (iii) HYPOTHETICAL: NO
iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~:~ (vii) IMMEDIATE SOURCE:
- (B) CLONE: TGLA170 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID N~: 26,:
TGTGTGAAAACAATGTCGCTACTAGTA~GGACCTGCTG-A-AG_ACAG~
AA

.-/0 92/13102 PCl/I~S92/00340 ' ` ` ' 2~0~j83 -~.35-(2) INFORMATION FOR SEQ ID NO: 268:

~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 67 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iil) HYPOTHETICAL: NO
: ~iv) ANTI-SENSE: NO

~ ~vi) ORIGINAL SOURCE:
;- ~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA171 (up) ; ~ txi) SEQUENCE DESCRIPTION: SEQ ID NO: 268:

TCTGTCTCCAACAATCCCAACTAGTGAGTGTAGCAAGAACCCAATTACCC
CCAGGCCCTTTTAGTTA
2~ INFORMATION FOR SEQ ID NO: 269:

(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: lS base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single D) TOPOLOGY: linear ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
iv) ANTI-SENSE: NO

vi) ORIGINAL SOURCE
A) ORGANISM: Bos taurus ~::
~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA171 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 269:
, . . .
GTGTGTGTGTGTGTG

(2) INFORMATION FOR SEQ ID NO: 270:

(i) SEQUENCE CHARACTERISTICS:
~:: (A) LENGTH: 59 base pairs ~ (B) TYPE: nucleic acid :~ (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (gencmic) (iii) HYPOTHETICAL: NO
,~

W O 92/131~2 2 1 0 0 ~ 8 3 PCT/USg2/00340 "

(iv) ANTI-SENSE: NO
ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii~ IMMEDIATE SOURCE:
~B) CLONE: TGLAl7l (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 270:
GAGGAGCCTGATGGGGAGCCATGGGGTTGCAAAGAGTTGGACCTGAGTGA
CTGAACACG
~2) INFORMATION FOR SEQ ID NO: 271:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7l base pairs (B) TYPE: -nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
;- (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl/2 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 271:
CAGATTTCGCAAGCAACAAGAGGTCACCTAGTTGAATTA&AACTTTAGAT
TAACAGTGGGGTATGAGTGTT
(2) INFOR~ATION FOR SEQ ID NO: 272:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 38 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl/2 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ 'D NO: 2,2:
TGAGTGTGAGTGTG.GTGTGTGTGTGAGTGT&AGTG-G

./0 92/1310~ 2 ~ O ~ ~ ~ 3 PCT/US92/0034o -2 ~7 -(2) INFORMATION FOR SEQ ID NO: 273:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(3) CLONE: TGLA172 ( down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 273:
CATGGACAGAGGAGCCTGGCAGGCTACAGTCCTTGGGGTCTCAAAGAGTC
AGACACTACTGAGCAT
(2) INFORMATION FOR SEQ ID NO: 274:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 62 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA175 (up) (xi) SEQUEN OE DESCRIPTION: SEQ ID NO: 274:
CCCATGGGTTTGCACAGGTTCATGAGACAGTCCGTGAATGGCTGGGCTTG
TATATGGTTGTT
~2) INFORMATION FOR SEQ ID NO: 275:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 43 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linea-(ii) MOLECULE TYPE: DNA ( genomic (iii) HYPOTP.E~ICAL: NO

WO g2/13102 2 1 0 0 ~ S 3 PCr/US92/00340 _23~-(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A~ ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA175 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 275:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 276:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA175 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 276:
GGGATATCAAAGCCAGAAAAAGGCAGGGGTATACCCCAGTGTAGAGACTG
TCTACT
(2) INFORMATION FOR SEQ ID NO: 277:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 68 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: singl~
~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA1,6 (up) (xi) SEQUENCE DESCRI~TIO~': SEQ I3 NO: 2,l:
TGAGCAGGGTTGTTCTGGGTGAGAAATGGGTAGTAGGAATAC?--CAGG.;
GGAAGTGGTGTGAGCAAT

`.~0 92/131~2 2 1 ~ ~ ~ g 3 PCT/US92/00340 (2) INFORMATION FOR SEQ ID NO: 278:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomuc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl76 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 278:
TGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 279:
(i) SEQUENCE CHARACTERISTICS:
~:~ (A) LENGTH: 54 base pairs :~: (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~: (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~ iu) ANTI-SENSE: NO
-: (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
B) CLONE: TGLAl76 (down) ~xi) SEQyENCE DESCRIPTION: SEQ ID NO: 279:
TCCAGGCACCTATGCTTTAAGAACTGCTCTCAAGGCACAAGCACACAGTC
: AGGG
, ~2) INFORMATION FOR SEQ D NO: 280:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 53 base pairs (B) TYPE: nucleic acid ` ~C1 STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

W O 92/13102 2 1 ~ O ~ ~ 3 PCT/~ISg2/00340 - 2~ -(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl79 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 280:
TACTGATTCTAGGTTAAACTTTAATCAGCACACAGCTTCCCAGTTAGCTG
TTT
(2) INFORMATION FOR SEQ ID NO: 281:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vli) IMMEDIATE SOURCE:
(B) CLONE: TGLAl79 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ I3 NO: 281:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 282:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 69 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl79 (down) (xi) SEQUEN OE DESCRIPTION: SEQ ID NO: 282:

CCTCAGGGCAGTTTCCAAAAAAATGCTAAGCTGCATATGTGCTAGAAGTT
TGGTCAACCCACACCCTCC
(2) INFORYATION FOR SEQ ID NO: 283:

~0 92/13102 PCT/~S92~00340 210()~
- 2~ 1 -(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 89 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ?
~ii) MOLECULE TYPE: DNA (genomic~
(iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISN: Bos taurus (~ii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl82 (up) (xi3 SEQUENCE DESCRIPTION: SEQ ID NO: 283:
TTTGATGAGCGACGGCACGACAGCCACAGCAAGTGGGATGGATACACACA
AACGTTGGTTTCTTTTGGACTATGAGCCCACCTCTGTTC
(2) INFORMATION FOR SEQ ID NO: 284:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 62 base pairs (B~ TYPE: nucleic acid (C) STRANDEDNESS: single ~:~ (D) TOPOLOGY: linear ; -:
~ (ii) MOLECULE TYPE: DNA (genomic) ¦~ (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl82 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 284:
GTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGAGAGAGA
~ (2) INFORMATION FOR SEQ ID NO: 285:
-~ (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: ll8 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (geno~.ic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

W O 92~13102 2 1 ~ 0 5 ~ 3 P~T/Us92/00340 ' - 2 ~ ~-(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl82 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 285:
GAAACAGTCCACGTCGTCCTCCAACTCATTGGACCATCACGACTGAAATG
AAGGAAAATATGTTCGTTCGAGAATGTCTTCACTCCTCAGTGCTTCTTGC
TTTAGAACCACCAAGGCA
(2) INFORMATION FOR SEQ ID NO: 286:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 85 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ID) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLAl88 (up) ;~ (x1) SEQUENCE DESCRIPTION: SEQ ID NO: 286:
CATGTGACTGTTCCTCATCTGTGTATCCTTCATTGAA...AATATCTAT-CAGGTCATCTGCCCTATTTTTTAATTCCAAACCTA
~2) INFORMATION FOR SEQ ID NO: 287:
: ~i) SEQUENCE CHA~ACTERISTICS:
(A) LENGTH: 17 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) iTOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
- (vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLAl88 kepeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28~:
TGTGTGTGTGTGTGTGT

NO 92/t~102 PCT/US92~00340 2100S~3 - 2 ~ 3-t2~ INFORMATION FOR SEQ ID NO: 288:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 68 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear : ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~: : (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~., vii) IMMEDIATE SOURCE:
(8) CLONE: TGLA188 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 288:
GATCTTTGCAAATGGTATTTCTGATAAGGGGTTAATATCAAAAATATATA
r~r~r ~ r ~ ~ rrr ~
~;~ A~ AA~A.A_A~A
2) INFORMATION FOR SEQ ID NO: 289:
~., :
~ , , ~i) SEQUENCE CHARACTERISTICS:
A) LENGTH: 76 base pairs B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(1v) ANTI-SENSE: NO
vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus vii) IMMEDIATE SOURCE:
(B~ CLONE: TGLA1~9 (up) ~,, j , ~ ...... .. . .
:~ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 289:
,~"~-~ TTTGGGAAACTAAATGCCTCCTCCTAATCCTGAGGATGAATTTGTAGTTA
AGGTGCTCACTATTTCTGTGGGAAAA
2) INFORMATION FOR SEQ ID NO: 290:
(i) SEQUENCE CHARACTERISTICS:
A) LENGTH: 18 base pairs a) TYPE: nucleic acid (C) STRANDEDNESS: single D~ TOPOLOGY: linea~
ii) MOLECULE TYPE: DNA (genomic) : .
~ ~iii) HYOTHETICAL: NO
- .
:, 21005~3 W O 92/13102 PCT/US92!00340 '~
;

- 2 L~
(iv) ANTI-SENSE: NO
(vi~ ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus r (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA189 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 290:
GTGTGTGTGAGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 291:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(3) CLONE: TGLA189 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 291:
AACTGCCGAAAACATTCTGTGAGCATGCTTTCACAA
(2) INFORMATION FOR SEQ ID NO: 292:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 77 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE: '~
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA2 ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 292:
ATGACCATGATTACGCCAAGCTTGGGCTGCAGGTCGACTC,AGA~uATC-TGCTACTGTCAGGACTGAACACTGCTC
~2) INFORMATION FOR SEQ ID NO: 293:

~0 ~2/1310~ PCT/~IS92/00340 210031~3 ~ S_ (i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 21 base pairs (B) TYPE: nucleic acid '.
(C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
Yi ) ORIGINAL SOURCE:
(A~ ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA2 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 293:
TGTGTGTGTGCGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 294:
:`
(i) SEQUENCE CHARACTERISTICS:
: (A) LENGTH: 141 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sinqle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA2 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 294:
TGCTTGAGGAGTCAAAATGAGCTATGCTCCAGTAAAAGGGGAGCTCTCAA
ACAGCTTGCCAGTACAGATTTCGACAGCAGAGGCATTTTGCTGGCAGTTG
GGGAATATACTGGCATTCTCAAGAAACAAGAGCTTTTAGGG
~2~ INFORMATION FOR SEQ ID NO: 295:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 81 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOT~.ETICAL: NC
(iv) ANTI-SENSE: N~

W ~ 92/13102 2 1 0 0 ~ 8 3 PCT/US92/00340 '' - 2~-(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (~ii) IMMEDIATE SOURCE:
(B) CLONE: TGLA20 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 295:
TTTGTAGCAAAGTCAGTTGTAGTTTATTTATTCCTTGATTATGTATACTT
AATGTCAAACCCAGTTAAAATAGCAAGAGTT
~2) INFORMATION FOR SEQ ID NO: 296:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 60 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA20 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 296:
: TGTGTGTGTGTGTGTGTGTGTGTGTATATATATACATATATATATATATA
TATATATATA
(2) INFORMATION FOR SEQ ID NO: 297:
(i) SEQUENCE CHARACTERISTICS:
: (A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (~ii) IMMEDIATE SOURCE:
(B) CLONE: TGLA20 (down) ~xi) SEQUENCE DESCRIPTIO~: SEQ ID NO: 297:

GATCTGTTTGTGAAGTAACATTGTAAGTATTGGGGAAA~
(2) INFORMATION FOR SEQ ID N^: 298:

'; ' ! ' , ' hlO 92/13102 PCI/US92/00340 21~0~3~
~ ~7 -(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 95 base pairs ~B~ TYPE: nucleic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TSLA203 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 298:
TTGGACTCTCAAGGCAATTGGGACTGAAGATGCCCTGGGGTCCCCACACA
AGCTCCTATCCTAATTACACATGAGGAACCCCAGGTGTTAATGAC
~2) INFORNATION FOR SEQ ID NO: 299:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 76 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (qenomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
- ~A) ORGANISM: 80s taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA203 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 299:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTGGTGGTGGTGGCAA
TCAGTCAATTTTTTTTTTTTTTTTTT
(2) INFORMATION FOR SEQ ID NO: 300:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 110 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

21005133 ~
W 0 92/13102 PCT/~TS92/00340 - 2~ -(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus tvii) IMMEDIATE SOURCE:
(B) CLONE: TGLA203 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 300:
GCAGCTTAAAGAATGACTGAAATCATATCATTTGGTTCCATCCAGACAGC
CCCATTATGGTAGTGGGTGAAGAGGAAAGCAGACTAGGACCTTCAGTGAG
GGCAGAGAAA
~2) INFO~MATION FOR SEQ ID NO: 301:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 93 base pairs .(B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA206 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 301:
TTGTACAGTTAAGCAAACCATCAACAAAACAAPAANNNNNGCCTACTGAA
;~ TGGGAGAA&ATATTTGCAAGCGTTACGCTCGAAAGGCTTAAGA
(2) INFORMATION FOR SEQ ID NO: 302:
- (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 48 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ,(D)i TOPOLOGY: linear ; (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (Yii) IMMEDIATE SOURCE:
(B) CLONE: TGLA206 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 302:
TGTGTG.GTGTGTGTGTGTGTGTGTGTG-GTGGAGAGAGAGAG-AuAGA

~0 92/13102 PCT/~'S92!00340 ~10~

- 2 ~9 -(2) INFORMATION FOR SEQ ID NO: 303:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 131 base pairs tB) TYPE: nucleic acid 'r (C) STRANDEDNESS; si~gle ~D) TOPOLOGY: linear , (ii) MOLECULE TYPE: DN~ (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
- (B) CLONE: TGLA206 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 303:
TTGATAGCCATTCTGAGTGGTGTGAGGGGATACGGCATTGGGATTTTGAC
TTCCATTTTCCTGATAATTAGTAATGTCCAGCCCATTTACATGTGCCTAC
AGACCACTGGCATGTGGTAGTCATACCTTCA
(2)-INFORMATION FOR SEQ ID NO: 304:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 164 base pairs ~: (B) TYPE: nucleic acid - ~ (C) STRANDEDNESS: single (D) TOPO~OGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~: (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
,~ j (B)I CLONE: TGLA208 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 304:
~: - TGGTGTGCTGCAGTCCATGTTACACAGAGAGTCAGACAATACTGAGCAAC
TGAATTGAACTTTGCCATAGGGAGAATGCTTTACAAGTTCTTCACATGTA
TCAAAATAA~ACAGAAAAAGATGTTTCTTTAACATGGAAGGATAAGCAGG
:- GCAAGCAACAACTC
~ (2) INFORMATION FOR SEQ ID NO: 305:
1:' (i) SEQUENCE CHARACTERISTICS:
~: (A) LENGTH: 42 base pai~s (B) TYPE: nucleic acid : (C) STRANDEDNESS: single : (D) TOPOLOGY: linea:
(ii) MOLECULE TYP-: DNA (genor.ic) W O g2/13102 21~ 0 5 8 3 PCT~US92/0034~

- 2 ~-(iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM, Bos taurus ~vii~ IMMEDIATE SOURCE:
(B) CLONE: TGLA208 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 305:
TGTGTGTGTGTGTGTGTGTGTGTGTG.TGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 306:
i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 159 base pairs ~B) TYPE: nucleic acid tC) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi3 ORIGINAL:SOURCE:
: (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B~ CLONE: TGLA20~ (down) (xi~SEQUEN OE DESCBIPTION: SEQ ID NO: 306:
~CCACTGACTTGGCTTGGCTAAACTTTAGCTAAGCTTCTCTCCTCTGAACT
;.-,c-~-TCAGCTTTCCCCTAAGCTTGAGCAAGCACTAAGAGCCAGGCTTTTCTGTC
TCCCTTAATAGCTCATTTTGGGAATCAACTCACTACAGAGAAAAATACTT
TCTGTTAAA
2) INFORM~TION EOR SEQ ID NO: 307:
'~1(i) SEQUENCE CHARAC~ERISTICS:
:::(A) LENGTH: 125 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single D) TOPOLOGY: linear , . ( MOLECULE TYPE: DNA (genomic) '~
-~-; (iii) HYPOTHETICAL: NO
~ ~ (iv) ANTI-SENSE: NO
, (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~:(vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA210 ~up~

92/13102 PCT/~s92/00340 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 307:
TTTCACAGTTGTCTAAATAAGAGAGTTATAATCACCCCACCCCCAGGTCA
TGGTCTAGTGCTCTTCTTCCAGAAAAATCCAATCTAAGCATTTGGGTGAA
GGGGGTCTGGCTGAAGACAACAGGA
(2) INFORMATION FOR SEQ ID NO: 308:
(i) SEQUENCE CHARACTERISTICS:
(A) L~NGTH: 27 base pairs (B~ TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOUROE:
(A) ORGANISM: ~os taurus ~vii) IMMEDIATE SOUROE:
~B) CLONE: TGLA2l0 ~r~peat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 308:
TGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 309:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 133 base pairs ) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus ~vii) IXMEDIATE SOURCE:
(B) CLONE: TGLA210 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 309:
TCTAGAGGATTCCCTGGAGAAGAGAATGGCAACCCACTCCAGTATTCTTG
CCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGTTATAGTCCATGAGG
TTGCAAAGAGTCAGACAGGACTGAATGACTAAT
~2) INFORMATION FOR SEQ ID NO: 3l0:

(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 183 base pai~s (~) TYPE: nucleic acid (C) STRANDEDNESS: slngle W O 92/13l02 2 1 ~ 0 5 ~ 3 PCT/US92~00340 ' _ 2S2-(D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) ~YPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA213 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 310:
GTGCTCTATCACAGCAAGTAAGGAATCACAGTCAAGTCACTAGAGGGCA&
AAGGACCTTGATAACTAGGCAGAGCTTTGCTCCATCTTAGAGTTCCTTAG
TGACCCTTGCTGAAGCAGAAATCTGTTG~AGAAAGGCTACTTTAGGCCTG
TTGGTGATAATGTGTGAATAGGAGCTCTGGTTT
(2) INFORMATION FOR SEQ ID NO: 311:
(i) SEQUENCE CHAR~CTERISTICS:
(A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYP-: DNA (genomic) (iii) XYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus : (vii) IMMEDIATE SOURCE:
tB) CLONE: TGLA213 (repeat) -~ (xi) SEQUENOE DESCRIPTION: SEQ I~ NO: 311:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
!~2)' INFORMATION ~OR SEQ ID NO: 312:
- (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 174 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single '~
~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic!
(iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOUROE :
~A) ORGANIS~.: 3Os taurus ~0 92/13102 PCT/~'S92/00340 ~10~3 -2 ~3 -(vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA213 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 312:
TGGAGAATCCCATGGAAAAAGGAGCCTGATGGGCTACAGTCTATGGGGTC
GTAAAGAGTCAGACACGACCGAAGCGACTTAGCACACACTGAGTATTTAT
CCGTTTAGAAAAATCATCACACTAAAAATTGTGCCCACAGGAGAGTTTGC
TCATCCTTCTACTGATTCAAA...
~2) INFO~MATION FOR SEQ ID NO: 313:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 1~7 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: line~r (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA214 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 313:
TTCCCTTAAAAAAGAGAGCTTTTTGAAGGTTTTGAGAGAATTGAACCTAT
TTTCNNCCTTAGACTGGA~TTACAATGTGAATGTATGTGCATGTGTGATG
CTTAGTGGCTCAGTTCGTGTGCCCAACTCTTTGTAACCCTATGGACTACA
GCCTGCCAGGCTTCTCTGTCCATGAAATGTTCCAGGCAAGAATACTG
~- (2) INFORMATION FOR SEQ ID NO: 314:
: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 22 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA214 (repeat) (x~) SEQUENCE DESCRIPTION: SEQ ID NO: 314:

GTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 31~:

WO 92/13102 2 1 0 0 5 8 3 PCr/US92/0~340 _ 25'~--(i) SEQUENCE CHARACT~RISTICS:
(A) LENGTH: 52 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ', (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (~ii) IMMEDIATE SOURCE:
(B) CLONE: TGLA214 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 315:
TTCTTGGGGCTTCCCAGGTAGCTCAAATGGTAAAGAATCAGCAAAGAGTC
TA
(2) INFORMATION FOR SEQ ID NO: 316:
(i) SEQUENCE C~ARACT~RISTICS:
(A) LENGTH: 88 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: .NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii). IMMEDIATE SOURCE:
(B) CLONE: TGLA215 (up) (xi) SEQU,ENCE DESCRIPTION: SEQ ID NO: 316:
: TTCATCCAGTGTTAAAAAAGGAAAAAGGAACCGTCATTTGTTTAGCATCA
GCCACGTGCCAGGCTAGGTGATGCACTTTATACACTTG
~2) INFORMATION FOR SEQ ID NO: 317:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(i~) ANTI-SENSE: N3 W O 92/~3102 P~T/US9~/00340 2 1 û D j g 3 .
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) I~MEDIATE SOURCE:
(B~ CLONE: TGLA215 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 317:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGT
(2) INFORMATION FOR SEQ ID NO: 318:
(i) SEQUENCE CHARACTERIST I CS:
(A) LENGTH: 73 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) T020LOGY: linea-(ii) MOLECULE TYPE: DNA (genomic) ~:~ (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
- (vi) ORIGINAL SOURCE:
~: (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA215 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 318:
ATGGGCAGAGGAACCTGGTGGGCTACAGTCCATGGGGCTGCAAAGAGTCA
TACATAACTGAGTGACTGAGCAT
(2) INFORMATION FOR SEQ ID NO: 319:
(i) SEQUENCE CHARACTERISTICS:
~ : (A) LENGTH: 100 base pairs ;~ ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~ (iii) HYPOTHETICAL: NO
:~ (iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~: (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA22 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 319:

TCAGTGAACTCAGGAGAGGTAAGCAAAAGAGATTCAAGCAGCT^-AGAATS
TTGTCTTAAGCTTCAATTGCAAGTAAAuGTmn`CTTGACTCAGG.;TAT.~

. ~ .

WO 92/13102 2 1 0 0 5 8 3 PC~/US92/00340 --2~ ~--(2) INFORMATION FOR SEQ ID NO: 320:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi~ ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA22 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 320:
TGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 321:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 52 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(8) CLONE: TGLA22 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 321:
GTGAGCTGCAGTTTATGGGGTCCCAAAGATTTGGACACAACTGAGCCACT
AA
~2) INFORMATION FOP~ SEQ ID NO: 322: ., (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pal_s (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii3 MOLECULE TYPE: DNA ~genomlc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENS_: NO

W O 92/13102 PCT/US9~/00340 2~0 ~3 - 2 5 7~
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA222 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 322:
VECTOR-GATCACTCTCTTAACA
(2) INFO~MATION FOR SEQ ID NO: 323:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linea (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA222 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 323:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 324:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 118 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single : (D) TOPOLOGY: lin~ar (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) C~ONE: TGLA222 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 324:
TTGAAGAAGGAAATGGCAACCCACTCCAATGTTCTTGCCTGGAGAATCC~
ATGGATGGAGGGGCCTGGTAGACTACAGTCTATGGGGTTTCA~GAGTTG
GACACAACTAAGCAACTA
(2) INFORMATION FOR SEQ ID NO: 325:

W O 92/13102 2 ~ O ~ 5 8 3 PCT/US~2/00340 - 2 S ~ ~

~i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: lsa base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
tA) ORGANISM: Bos taurus -: ~vii) IMMEDIATE SOURCE:
:- ~B) CLONE: TGLA226 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 325:
CTCAGAAAGAACTTCAGATTACACTTTGGGGAAACAGGAAAGCACTGGAG
GACAGTTGGACCTGACATTTTAACAAAGGTCTTGGCTCCTGAGTGGAATC
: ~ CAGATAAGATGTATCAAAAAGTTTGTAACGCTTTTATTTTTTTAAAGTTT
(2j INFORMATION FOR SEQ ID NO: 326:
(i) SEQUENCE CHARACTERISTICS:
Aj LENGTH: 31 base pairs : : (B) TYPE: nucleic acid : ~ (C) STRANDEDNESS: single (D) TOPOLOGY: linear .::
~ ~ (ii) MOLECULE TYPE: DNA (genomic) -, :
~ ;;: (iii) HYPOTHETICAL: NO
;, ~
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
¦ (B) CLONE: TGLA226 (repea~) xi) S~QUENCE DESC~IPTION- SEQ ID NO: 326:
:~ TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
- :-2) INFORMATION EOR SEQ ID NO: 327:

(i) SEQUENCE CHARACTERISTICS:
~ : (A) LENGTH: 64 base pairs -~ (B) TYPE: nucleic acid (C) STRANDEDNESS: sinsle ~: (D) TOPOLOGY: linear ;~ (ii) MOLECULE TYPE: DNA (genomic) :~ (iii) HYPOTHETICAL: NO
(iv) ANTI-SENS_: NO

WO 92/13102 PCI`/US9~/00340 2100~
_ ~5 ~ ~

~vi) ORIGINAL SOURCE:
~A) ORGANISM: 30s taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA226 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 327:
AAAACCAAACATGAAAAGAAGCAATATCGTAACAAATTCAATAAAGACTT
TA~AAATGGTCCAT
~2) INFORMATION FOR SEQ ID NO: 328:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA227 (up) txi) SEQUENCE DESCRIPTION: SEQ ID NO: 328:
VECTOR-GAATTCCAAATCTGTTAATTTGCT
(2) INFORMATION FOR SEQ ID NO: 329:
(i) SEQUENCE CHARACTERISTICS:
:~ (A) LENGTH: 39 base pairs - ~ (B) TYPE: nucleic acid : (C) STRAN~FDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
¦~ (B) CLONE: TGLA227 (repeat) ¦ (xi) SEOUENCE DESCRIPTION: SEQ ID NO: 329:

TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG-(2) INFORMATION FOR SEQ ID NO: 330:

- 2f4~Q--( i ) S r QUENCE CHARACTERISTICS:
(A) LENGTH: 87 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA227 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 330:
VECTOR-GAATTCAAAATAAATACTTGAAGGAAACTCAGTCAGCTCCAAG
AGAACACAGACAGAAACTCAATGAAAGCAGGAAACAT
(2) INFORMATION FOR SEQ ID NO: 331:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~: (vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os ~aurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA23 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 331:
AAGGGGTGCTGTGGTACATCAGGGCAATAGTCAGAGTCCAGGACCATTCA
TGCAAACTGAGACACAAGCTTTCAACCACCACCTATATTAAC
(2) INFORMATION FOR SEQ ID NO: 332:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 38 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYP~: DNA ~genom~

(iii) HYPOTH-TICAL: NO
(iv) A~'TI-SENS_: NO

WO 92~13102 PCI/US92/0~340 2100~3 - 2~l-(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA23 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 332:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 333:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 63 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~il) MOLECULE TYPE: DNA (genomic) liii) HYPOTHETICAL: ~O
~ (iv) ~NTI-SENSE: NO
:~ (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus - (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA23 (down) ~;~ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 333:
~: CCTGGAAAACTCATCTTTAATCATTTGCCCCAGTTGATTTAT.AAAAACA
AATGCATTAAATA
(2) INFORMATION FOR SEQ ID NO: 334:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 44 base pairs : ~B) TYPE: nucleic acid : (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) I i (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
: (vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA231 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 334:

I GGCGAGCTCGAATTCTCCATTTCCCTTTGGTTTGTAAAGACA^--(2) INFORMATION FOR SEQ ID N^: 335:

~ (i) SEQUENCE CHARACT~R-S,ICS:

WO 92/13102 PCr/US92/00340 --2~ ~ -~A~ LENÇTH: 26 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus - (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA231 (repeat) ~ ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 335:
- TGTGTGTGTGTGTGTGTGTGTGTGTG
~2) INFORMATION FOR SEQ ID NO: 336:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 119 base pairs B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ,~
; (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus vii) I~MEDIATE SOURCE:
B) CLONE: TGLA231 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 336:
~: VECTOR-GAATTCAAATTCTCGCAGCACATTTTAATCATCTACCTGCTGu CAATTAACTAACATTAAGACTGCTGCAAAGAGTTGGACAGAACTGAGCAA
. CTGAGCACACGCGTGCGTG
~2) INFORMATION FOR SEQ ID NO: 337:
-~: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 45 base pairs ;~ : (B) TYPE: nucleic acid ~: ~C) STRANDEDNESS: single (D) TOPOLOGY: linear : (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAl: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:

- 2~ 3- 210~3 t (A) ORGANISY.: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA245 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 337:
TTTTTGCATCTATGTTCATCAGTGATATTGGCCTGTAGTTTTCTT
~2) INFORMATION FOR SEQ ID NO: 338:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 47 base p2' rs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA245 kepeat) (xi) SEQUENCE ~ESCRIPTION: SEQ ID NO: 338:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
- ~ ~2) INFORMATION FOR SEQ ID NO: 339:
~- (i) SEQUENCE CHARACTERISTICS:
A) LENGTH: 107 base pa~rs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
l (yi) ORIGINAL SOURCE:
¦~ (A) ORGANISM: Bos taurus (vii~ IMWEDIATE SOURCE:
1 (B) CLONE: TGLA245 ~down) ¦: (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 339:
GCTAATGCTTATCCTTCTAAAACTCTTTCAAAAAAATTGCA&&-GAAAGA
ACACTTCAAAACTCATTCTATAAGGCCACCATCATCCC.~TACCAAAACC
AGACAAA

(2) INEORMATION FOR SEQ ID NC: 340:
(~) S~QUENC~ CHARACTERIS-ICS:

WO 92/13102 P~/US9~/00340 - 2(~
(A) LENGTH: 70 base pairs tB) TYPE: nucleic acid (C~ STRANDEDNESS: sinyle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) liii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~ ~vii) I~MEDIATE SOURCE:
:~ (8) CLONE: TGLA25 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 340:
AGGGTGCAAAGAGTTGGACACGACTGAGCAAGTGAACTGAACTGATTGTG
TGTGTATAGCTCACCTATAT
~(2) INFORMATION FOR SEQ ID NO: 341:
i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 26 base pairs ` tB) TYPE: nucleic acid tC) STRANDEDNESS: single (D) TOPOLOGY: linear :ii) MOLECULE TY~E: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
tA) ORGANISM: Bos taurus vii) IMMEDIA~E SOURCE:
B) CLOUE: TGLA25 (repeat) (x~) SEQUENCE DESCRIPTION: SEQ ID NO: 341:
TGTGTGTGTGTGTGTGTGTGTGTGTG
: ~2) INFORMATION FOR SEQ ID NO: 342:
: (i) SEQUENCE CHARACTERISTICS:
tA) LENG~: 46 base pairs : t3) TYPE: nucleic acid tC) STRANDEDNESS: single D) TOPQLOGY: linear (ii) MOLECULE TYPE: DNA tgenomic) ~; (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
-,, , ~
. ~vi) ORIGINAL SOURCE:
(A) ORGANISM.: 30s tau-us WO g2~13102 PCT/US92/00340 2 1 0 ~
- 2~ 5 -~vii) I~MEDIATE SOURCE:
~3) CLONE: TGLA~5 ~down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 342:
AATATTTCTTTATGAACTGGTAAATAAACAGAAGGTGAGACCCATG
(2) INFORMATION FOR SEQ ID NO: 343-(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: ~2 base pairs (B) TYPE: nucleic acid ~C) S~RANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii~ IMMEDIATE SOURCE:
(B) CLONE: TGLA254 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 343:
TTTATGGCTGAGTAATAATCCG
(2) INFORMATION FOR SEQ ID NO: 344:
(i) SEQUENCE CHARACTE~ISTICS:
(A) LENGTH: 47 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA254 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 344:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 345:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 178 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single W O 92/13102 2 1 ~ U 5 8 3 PCT/USg2/00340 -2 ~~
(D) TOPOLOGY: linear (ii) MOLECULE TY~E: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA254 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 345:
AATCCATCATAACTGTACAAGACTCATTTACTATGAGGCATATATCTTCA
TAAAGTAACCAAAATTGAAAAAGGCACATGTATGACAATGTTCTATGCAG
CTCTATTTACAATAGCCAGACATGGAAGCAACCTAGATGTCCATCAACAG
ATGAACAGATAAAGAAGCTGTGAGATAT
(2) INFORMATION FOR SEQ ID NO: 346:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 52 base pairs ::: (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear - (ii) MOLECULE TYPE: DNA (genomic~
(iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus - (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA255 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 346:
CAGTGTAGCTCTCTGGGCCCAGACAGAGGGGTAAGGCTGTGTG~ATGTCG
GG I
(2) INFORMATION FOR SEQ ID NO: 347:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT~: 28 base pairs (B) TYPE: nucleic acid - (C) STRANDEDNESS: single ¦ (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: 3Os tau-us I

W O 9~/13102 . . PCT/US92~00340 2100~
- 2~7 - j (vii) IMMEDIATE SOURCE: !
(B) CLONE: TGLA255 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 347:
GTGTGTGTGTGTGTGTGTCTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 348:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 82 bzse pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A~ ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA255 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 348:
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC
GACGTTGTAAAACGACGGCCAGTGAATTCCTA
(2) INFORMATION FOR SEQ ID NO: 349:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 90 base pairs tB) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLEC~LE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(~i) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IM~EDIATE 50URCE:
(B) CLONE: TGLA257 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 349:
AAAAATCACAGGGCTGCTGGAAACCACCTGTGATCTCTCTCCAC~TTTCC
CCTATCATTCACATAACTCTTCTCATATAGCCCAGAAATA
(2) INFORMATION FOR SEQ ID NO: 350:

~i) SEQUENCE C~ARACTERISTICS:
(A) LENGT~: 31 base pa_~s WO 92/13102 PCl/US92~00340 --2~-IB) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA257 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 350:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 351:
(i) SEQUENCE CHARACTERISTICS:
. ~A) LENGTH: 43 base pairs ~B) TYPE: nucleic acid - (C) STRA~DEDNESS: single (D) TOPOLOGY: linear (ii) MQLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~-(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA257 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 351:
ACTACAGTCCATGGGGTTGCAAAGAGTCAGACACGACTGACTA
(2) INFORMATION FOR SEQ ID NO: 352:
. I
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 167 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv~ ANTI-SENSE: NO

(vi) ORIGINAE SOURCr (A) ORGANISM: Bos taurus (vii) IMMEDIAT- SOURCE:

W O 92~13102 PCT/US92/00340 I

210~S~3 _ ~9-(B) CLONE: TGLA26 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 352:
TTTCTGAAGGTGTTATTAAGGTCGAGGACACCATTAAGCTCCATTTAATA
AAGGTTAGACTTTAACCCACCAATGTTCAG$TACTCTCTTCAGCAGTAGG
CTAATGACTGCTATTGGAGCGGGAGAAGAAGGAAGGCCCAGCCATCTACT
TTTCTGATATGTTGTCG
(2) INFORMATION FOR SEQ ID NO: 353:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 base pairs tB) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HY~OTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus tvii) IMMEDIATE SOURCE:
(B) CLONE: TGLA26 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 353:
TTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGT
GTGTGTGCGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 354:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: Sl base pairs (B) TYPE: nucleic acid tC) STRANDEDNESS: single (D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
I ~vi) ORIGINAL SOURCE:
¦ (A) ORGANISM: Bos taurus ¦ (vii) IMMEDIATE SOURCE:
¦ (B) CLONE: TGLA26 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 354:
GATCTGCCTCCAGATTTTGTTTATCCCCTCA&CCACATGACAGGTTTAGA
A

t2) INFORMATION FOR SEQ ID NO: 355:
~i) SEQUENCE CHARACTERIS-ICS:

W O 92/13102 2 1 0 0 5 8 3 PCT/US~2/00340 -27~-(A~ LENGTH: ~9 base pairs tB) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear 4 ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA260 (up) ~xi) SEQUENCE DESCRIPTION: S~Q ID NO: 355:
GTGTTGTGTGTTCTGGCTCTGAGACCTCTCCCTGACCCATCGATTTTTCC
CGTGTAGGATGGATTATTGACCTGTTCTGATTCAGTCTA
(2) INFORMATION FOR SEQ ID NO: 356:
: .:
(i) SEQUENCE CHARACTERISTICS:
~:~ (A) LENGTH: 98 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) , . ~., .
iii) HYPOTHETICAL: NO
iv) ANTI-SENSE: NO
,~
. ~ :
: ~vi) ORIGINAL SOURCE:
A) ORGANISM: ~os taurus : (vii) IMMEDIATE SOURCE:
~B) CLONE:: TGLA260 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 356:
T~TTTCCTTTTTTTTTTTTTTTGATGGGGTGGGCCTCTGTTAATTTGGTG
ATTCCAAATAAATTCTGTGTGTGTGTGTGTGTGTGTGTGTGTG~GTGT
(2) INFORMATION FOR SEQ ID NO: 357:
~: ~i) SEQUENCE CHARACTERISTICS:
: ~A) LENGTH: 122 base pairs :~ ~B) TYPE: nucleic acid (C) STRANDEDNESS: sinsle ; ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINA_ SOURC-:

WO 92J13102 PCI`/I,'S92~00340 210a~3 (A) ORGANISM: ~os taurus ~.
~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA260 (down~
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 357:
CTATTCCTTGCCCCAACAACTCATTTCTAAATCTATCGGCCTATTGTGCA
GTGAGCAGTAGGAGCTTGGACTGGTAGT~AATGTGAATGTGTTACTTTTT
TTCAGTCATATCTGACTCTGCA
~2) INFORMATION FOR SEQ ID NO: 358:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 147 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomicj ;
(iii) HYPOTHETICAL: NO
;: (iv) ANTI-SENSE: NO
(Yi) ORIGINAL SOURCE:
~- (A) ORGANISM: Bos taurus :~ (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA261 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 358:
~ CCCTATCTTGCTGAAGTCCTTCAAATCTTTGCTCAAATCTCATTCTCTCC
-`~ AGAAGGCCTAACCTTACCACTTCCACCCTCAACACAAGCATAAAAACTGA
CTGTCCTGATGCTTTTAACACTGTCCTATTTCTATGGCCTTATCTTC
~(Z) INFORMATION FOR SEQ ID NO: 359:
: (i) SEQUEN OE. CHARACTERISTICS:
(A) LENGTH: 40 base pairs ~ ) TYPE: nucleic acid - (C) STRANDEDNESS: single (D) TOPOLOGY: linear , :
(li) MOLECULE TYPE: DNA (genomi c (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
A) ORGANISM: Bos taurus ~ii) IMMEDIATE SOURCE:
~B) CLONE: TGLA261 kepeat) ;~ (xi) SEQUENC~ DESCRIPTION: SEQ ID NO: 35~:
TTATATATATATATATATATATATATATATA~ATATAT~
(2) INFORMATION FOR SEQ ID NO: 360:
.1 W O 92/13102 2 1 0 0, 8 ~ PCT/US92/00340 -~7 2 -(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 135 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sin~le ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A~ ORGANISM: 30s taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA26l (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 360:
AATACATCTCAGACTGAGCTATTTGATTAATGCATTCACTTCATTCATCC
AACTTATATTAGGCACAATGTCCTTGGG...TTTCTAGATGTACTAACTC
TAAGTGAGTAGAGAGAATTAACAAAGCTTAGTAAG
(2) INFORMATION FOR SEQ ID NO: 361:
(i) SEQUENCE CHARACTERISTICS:
: (A) LENGTH: 79 base pairs (B) TYPE: nucleic acid : (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMNEDIATE SOURCE:
(B) CLONE: TGLA263 (up) '~ (xi) SEOUENCE DESCRIPTION: SEQ ID NO: 361:
CCCGGCGAGCTCGAATTCCTGTTTGGAGCAGAAGCAGGAAAGCAAGTGCT
- GGATACTATCTGAGCAAATACAGAGAAAA
; (2) INFORMATION FOR SEQ ID NO: 362:
(i) SEQUENCE CHARACTERISTICS:
IA) LENGTH: 58 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linea-(ii) MOLECUTE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO

W 0 92/13102 PCTtUS92~00340 2100~3 _ ~73-(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A~ ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA263 ~repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 362:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGGGGGTGTGTGTGTGTG
TCTGTGTG
~2) INFORMATION FOR SEQ ID NO: 363:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 4l base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear ~il) MOLECULE TYPE: DNA ~genomic) :~. (iii) HYPOTHETICAL: NO
.
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A3 ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA263 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 363:
`~ ' GCCCTCACCAATATTTAAAGCATCCTCACCTATATATGCCT
~-~ (2) INFORMATION FOR SEQ ID NO: 364:
: : `
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single D) TOPOLOGY: linear (ii) MOLECU~E TYPE: DNA (genomic) iii) HYPOTHETICA1: NO
:~ (iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
: (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA264 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 364:
ACTGGGCTTTTCTCTCAAACCCCAACCGTAAGCCCAGTCmGG^-GAGT~-GAGCAA

W O 92/13102 2 1 0 0 ;~ ~ 3 PCT/U$92/00340 ~ 27 ~ -~2) INFORMATION FOR SEQ ID NO: 365:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE: .
(B) CLONE: TGLA264 ~repeat) (xi) SE`QUENCE DESCRIPTION: SEQ ID NO: 365:
GTGTGTGTGTGTGTGTGTGTGTGTG
~2) INFORMATION FOR SEQ ID NO: 366:
(i) SEQUENCE CHARACT~RISTICS:
(A~ LENGTH: 65 base pairs (B) TYPE: nucleic acid . (C) STRANDEDNESS: single ~ (D) TOPOLOGY: linear :~ (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~: (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA264 (down) (xi) SEQpENCE DESCRIPTION: SEQ ID NO: 366:
TTCCCAGTCACGACGTGTAAAACGACGGCCAGTGAATTCAGCAGTTATC~
TTCCTTCCCCCAGGCT
(2) INFORMATION FOR SEQ ID NO: 367:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomi-) :~
~: (ili) HYPOTHETICAL: NO
(iv) ANT;-SENSE: NC

~lOOi3~ ~
~ ~ s _ (vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
- (B) CLONE: TGLA268 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 367:
CCCGGGCGAGCTCGAATTCTATGCT
(2) INFORMATION FOR SEQ ID NO: 368:
~i) SEQUEN OE CHARACTERISTICS:
(A) LENGTH: 56 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
: (vi) ORIGINAL SOURCE:
~; (A) ORGANISM: Bos taurus ::~. (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA268 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 368:
TGTGTGTGTATTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTG
~- TGTGTG
~:~ (2) INFORMATION FOR SEQ ID NO: 369:
(i) SEQUENCE CHARACTERISTICS:
~: (A) LENGTH: 54 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: lLnear (ii) MOLECULE TYPE: DNA (genomic) : (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~: (A) ORGANISM: ~os taurus ~vii) IMMEDIATE SOURCE:
- (B) CLONE: TGLA268 (down) ~xi) SEQUENCE DESCRI~TION: S~Q ID NO: 369:
TG&AATAAP~ACATAGATGACGGAGTTTTCCTGATCCTmCCTCT ~ ACACr~
TTCT
(2) INFORMATION FOR SEQ ID NO: 370:
_ . ....... . .

2100583 ~
W O 92/13102 PCT/us92/~0340 _27~-(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 53 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TG'A27 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 370:
CTCATGCTGCACAACTAGGATTGAACTATCTCTTGGTATATCTCATGCTA
ATC
(2) INFORMATION FOR SEQ ID NO: 371:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA27 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 371:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2~ INFORMATION FOR SEQ ID NO: 372:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 69 base pairs (B) TYPE: nucleic acid i(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~(iii) HYPOT~ETICAL: NO
;s~iv) ANTI-SENSE: NO

W ~ 92/13102 PCT/US92J00340 210~
- 2 7 7 ~
~vi) ORIGINAL SOURCE: . ;
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
' tB) CLQNE: TGLA27 (down) - (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 372:
ATCTCATGGACAGAGGAGCCTCTGCAGGCTGCAGTCCACGGGGTCGCAAA
GAGTCGGACACAACTGAGC
~2) INFORMATION FOR SEQ ID NO: 373:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 29 base pairs (B) TYPE: nucleic acid : (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA272 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 373:
CTGGCGGTTAGAGGCTTGCACGCTGTAAA
(2)~INFORMATION FOR SEQ ID NO: 374:
;;,; ~ ~
'i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 54 base pairs ~-~- : (B) TYPE: nucleic acid C) STRANDEDNESS: single : (D) TOPOLOGY: linear ,~,, ~ .
~: (ii) MOLECULE TYPE: DNA (genomic) - , . (iii) HYPOTHETICAL: NO
, (iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~, (A) ORGANISM: Bos taurus :: ~ (vii) IMMEDIATE SOURCE:
:~ (B) CLONE: TGLA272 (repeat) , . (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 374:
TGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTG-GTGTGTGTG
TGTG
:~ (2) INFORMATION FOR SEQ ID NC: 375:
~, . W 0 92/13102 2 i O ~ S ~ ~ PCT/US9~/00340 - 2~

(i) SEQUENCE CHARACTERISTICS: .
(A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA272 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 375:
GATCAAAGCATGGAGGCCAGAGAGAGAGTGTGTGGG
(2) INFORMATION FOR SEQ ID NO: 376:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 54 base pairs : (B) TYPE: nucleic acid ~- (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~- (ii) MOLECULE TYPE: DNA ~genomic) ~: (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
. ~ ~
~ (vi) ORIGINAL SOURCE:
:~ (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE~ TGLA28 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 376:
: TCATTTTCTTCCTGCTTCTGTAGGTTCTTAGACTTAGTAAGTAGCTTTTC
TAAC
(2) INFORMATION FOR SEQ ID NO: 377:
~ (i) SEQUENCE CHARACTERISTICS:
:~ (A) LENGTH: 36 base pairs : (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ii) MOLECU~E TYPE: DNA (qenomic) : (iii) HYPOTHETICAL: NO
(iv) ANTI-SENS_: NO

W O 92!13102 PCT/US92/00340 210()~3 -279- ~
(A) ORGANISM Bos taurus l, (vii) IMMEDIATE SOURCE: ~
~B) CLONE TGLA28 (repeat) ~, (xi) SEQUENCE DESCRIPTION SEQ ID NO: 377:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
(2) INFORMATION FOR 5EQ ID NO: 378 (i) SEQUENCE CHARACTERISTICS
(A) LENGTH 93 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS single (D) TOPOLOGY linear (ii) MOLECULE TYPE D~A (genomic) ~iii) HYP OTHETICAL: NO
(iY) ANTI-SENSE NO
(vi) ORIGINAL SOURCE
(A) ORGANISM Bos taurus - (vii) IMMEDIATE SOURCE
j (B) CLONE TGLA28 (down~
(xi) SEQUENCE DESCRIPTION SEQ ID NO: 378 AGACAGTGGGAGTGGGAGTGTCTCTGAAGATGTTTGGCCTTTAGTTGATA
TGTCAGAGCTGGAATCAGACTCAGGTATCCTATCTAGATTAAA
(2) INFORMATION FOR SEQ ID NO 379 (i) SEQUENCE CHARACTERISTICS
(A) LENGTH 83 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS single (D) TOPOLOGY linear (ii) MOLECULE TYPE: DNA (genomic) ; (iii) HYPOTHETICAL NO
~, (iv) ANTI-SENSE NO
(vi) ORIGINAL SOURCE
(A) ORGANISM Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE TGLA3 (up) (xi) SEQUENCE DESCRIPTION SEQ ID NO 379 CATTTAGTCAGAGTA~AACAGTGATTTTTATACTTTTC.TTATAAATG~

TTTTTGAAGGCATTAAACTTAGAGAATCTACTT
~2) INFORMATION FOR SEQ ID NO: 38C
~i) SEQUENCE CHARACTERTSmICS:

S 8 3 ~
WO 92/13102 ~ PCl`/US92/00340 - 2~-(A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ~iii) HYPOTHETICAL: NO
(iv~ ANTI-SENSE: NO
(vi) ORI~INAL SOURCE:
(A) ORGANISM: Bos taurus (vii~ IMMEDIATE SOURCE:
~B) CLONE: TGLA3 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 380:
TATATATATATGTGTGTGTGTGTG
~2) INFORMATION FOR SEQ ID NO: 381:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 66 base pairs ~B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA3 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 381:
ACTGTGTATTTTATTCTATAAATAAAATTTGTATAGAGGTTTACATATAC
AGAATACAGAATAAAA
~2) INFORMATION FOR SEQ ID NO: 382:
(i) SEQUENCE CHARACTERISTICS:
(A~ LENGTH: 50 base pairs ~.
(B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURC-:
(A) ORGAN:SM: Bos ta~a-~_s W 0 92/13102 210 0 5 8 3 PCT/USg2!00340 I

_2 ~

(vii) IMMEDIATE SOURCE: I
- (B) CLONE: TGLA301 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 382:
AACTCTAGAGAAAAGCAGAATTTCAAAATCAAATGAATGGATGAAGAAGG
(2) INFORMATION FOR SEQ ID NO: 383:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 21 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(Yi) ORIGINAL SOURCE:
,~A) ORGANISM: Bos taurus - (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA301 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 383:
TGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 384:
(i) SEQUENCE CHARACTERISTICS:
: (A) LENGTH: 37 base pairs (B) TYPE: nucleic acid :~ (C) STRANDEDNESS: single - ~ (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
. , (iv) ANTI-SENSE: NO
~, (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA301 ( down) ~: (xi) SEQUENCE DESCRI~TTON: SEQ I3 NO: 384:
TGCAGATGGTGTTATTTCATTCTCTTTTATGGCTGT'T
(2) INFORMATION EOR SEQ ID NO: 385:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 145 base pai-s :: (B) TYP_: nucleic acid (C) STRANDEDNESS: single .

210058~ --W ~ 92/13102 PCT/US9~/00340 -~ 2-~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos tau~us (vii) IMMrDIATE SOURCE:
(B) CLONE: TGLA303 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 385:
GAATTCCCTGCAGGT~ATTAGACCAGTTCTTTCCCAGTATAGATAACAGT
GGCTTTTTCCACCACTTATTTTTAGGGCCTGCCTTTTTT~TTTCATATA~
TTATAATCATAAGTCAAAGTAACAGTTTAGATGTCCAGTTTGCTC
~2) INFORMATION FOR SEQ ID NO: 386:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 36 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
: (iv) ANTI-SENSE: NO
; (vi) ORIGINAL SOURCE:
:~ (A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA303 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 386:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
~2) INFORMATION FOR SEQ ID NO: 387:
(i) SEQUENCE CHARACTERISTICS:
: ~A) LENGTH: l99 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~, (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:

W O 92/131~2 2 1 0 0 5 8 3 PCT/US92/00340 (B) CLONE: TGLA303 (down) ; (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 387:
CCTCAGTAATGGTTTTGTTTTAAAACACTTTGTTTATCCTTAAAATTAAA
GCGTTTGAAAGAAAGAAAATGATGTAATTTGTAAAGTGTGGCCATTAATA
CAAGTGAACTTACAGATCTGGACATACAAAAGTATTACCTCAAGAGATGG
ACCCACTTGTGTGCCAGACCCAGGAATCCTATAGAGGTCATACAGGAGG
(2) INFORMATION FOR SEQ ID NO: 388:
~i)`SEQUENCE CHARACTERISTICS:
(A) LENGTH: 45 base pairs : ~ (B) TY?E:: nucleic acid C) S~RANDEDNESS: single (Dj TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii)~HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi):ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~: : (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA304 (up) (xi) SEQUENCE~DE~S~CRIPTION: SEQ ID NO: 388:
TTTTT~ GATGTCAATTGTTGATCTGTCAACCTTTCAATTGATTC
2)~INFORMATION FOR SEQ ID NO: 389:
(i) SEQUENCE CHARACTERISTICS:
tA) LENGTH: 23 base pairs (B3 TYPE: nucleic acid (C) 5TRANDEDNESS: single D) TOPOLOGY: linear (ii) MOLECULE TY?E: DNA (genomic) (iii) HYPOTHETICAL: NO
: (iv) ANTI-SENSE: NO
(Yi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus : (vii) IMMEDIATE SOURCE:
~: (B) CLONE: TGLA304 (repeat) .. ..
(x1) SEQUENCE DESCRIPTION: SEQ ID NO: 389:
` TGTGTGTGTGTGTGTGTGTGTGT

(2) INFORNATION FOR SEQ ID NO: 390:
(i) SEQUENCE CHARACTERISTICS:
(A) ~ENGTH: 43 base pairs (B) TYPE: nucleic acid WO 92/13102 219 0 ~ ~ 3 PCI/US92/00340 - 2~ -(C) STRANDEDNESS: single ~D) TOPOLOGY: linear ~ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ~NTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA304 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 390:
GATACCTAGGTGTAGAACTGAGGAGGGTCATTTATTTCTTTTA
(2) INFORMATION FOR SEQ ID NO: 391:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 199 base pairs (~) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA306 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 391:
GTTGAGAAATAACATTAATATCCATGGAACATTCAAAGATAAGACTGATG
ATCAAGTACAGCAGGCTGAGTTACACTGAATTTAAAATATACACAATCAT
GTGTTAGTAAAGGCATGTCCCATATAATATTTTTATTTGCTAAATCTGGC
CACCTTGCTCAAGAAACATTAGCATTTAATGTCATTATT.TCTAGAAGA
(2) INFORMATION FOR SEQ ID NO: 392:
I (i) SEQUENCE CHARACTERISTICS:
¦ (A) LENGT~: 22 base pairs ~, (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ¦ (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHE~ICAL: NO

(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURC-:
I (A) ORGANISM: 3Os ~aurus W O ~2~13102 2 1 0 0 5 8 3 PCT/US92iO0340 _~ 5~

(vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA306 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 392:
TGTGGATGTGTGTGTGTGTGTG
(2) INFORMATION FOR SEQ ID NO: 393:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 160 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPO~OGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA306 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 393:
TTGTGTTGATAGGGGTTTTTAATGAGTCTTGCTAGAAATGTATCAGTTAC
CTTCTCAGAGAGCCCGGCTTTGGCATCATGTGTTTCACTCTGTCACTTTC
TGTTTTCAGTTTCACTGGTTTCTGTCGTCACCTTCATTATTCCTTTCCTT
CTTCTTT...
~2) INFORMATION FOR SEQ ID NO: 394:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 141 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: s-ngle (D) TOPOLOGY: linear (ii) NOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA307 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 394:

GAATTCCCTTCAGTCCCTCATCTTTGCATTCTCATTTACCAAGAGGTCCG
TGACTCAGGTCCAGTGCAATCCATGTG.CATACAAGACAGGCAAGCAGAC
AAGGAAAGGTTACCATCCTuGACTTCCAAAGAu-.TGA...
~2) INFORMATION FOR S~Q ID N3: 39~:

WO 92/13102 ; 2 1 0 0 5 ~ ~ PCr/US92/00340 2a~--(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (O TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA307 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 395:
TGTGTGTGTGTGTGTGTGTGT
: (2) INFORMATION FOR SEQ ID NO: 396:
(i) SEQUENCE CHARACTERISTICS:
~ (A) LENGTH: 148 base pairs S~ : (B) TYPE: nucleic acid : (C) STRANDEDNESS: single (D) TOPOLOGY: linear : (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
`(iv) ANTI-SENSE: NO
, ....
(vi:) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (viij IMMEDIATE SOURCE:
) CLONE: TGLA307 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 396:

' ; AGGGCATGGATGGTCCTGGGAGTCGGTGGGASGTTATACGTTTGTCCAGA
GTCAACCTTGCCTGCCTCCATCTGATTTAGTTAGGACTAGAAGGAGGCAG
-:~ TCTGGGTAAATTTGCCTGGATGAATCCTGAACAGGCAAGGATCCCTAA
., 2) INFORMATION FOR SEQ ID NO: 397:
~ (i) SEQUEMCE CHARACTERISTICS:
;:~ (A) LENGTH: 69 base pairs : (B) TYPE: nucleic acid (C) STRANDEDNESS: single ~:- (D) TOPOLOGY: linear :::
(ii) MOLECULE TYPE: DNA (genomic) ~: (iii) HYPOTHETICAL: NO
4~ (iv) ANTI-SENSE: N~
~:

W 0 9~/13102 210 0 5 8 3 PCT/US92t00340 -2 ~1-(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA309 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 397:
ATTCAGTAGTCATATGACCTTTGTCCTTTGTATTCTGTGAAAGACATTCT
TTGTAGATAGCATGATAGG
(2) INFORMATION FOR SEQ ID NO: 398:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 38 base pairs (B~ TYPE: nucleic aci~
(C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
¦ (A) ORGANIS~: Bos taurus (vii) IMMEDIATE SOURCE:
~; (B) CLONE: TGLA303 ( repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 398:
GTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTTGTG
(2) INFORMATION FOR SEQ ID NO: 399:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 195 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA309 ( down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39,:

CATCAAAATGCTTACTAGCCCATCACTGAAAACACAATTTCG XTGCSCA
TCCAAACACCATTTTTT-CTAAACCTAGGA&&-CCAT-TC~;-A-.A-~.i ATATGACATAATTGTTCTGCCAGCATTACAGA,TTCATACAGCCGAu-G--AAT,TATAACTGAGGCAATTTACCATAATu&T~TAG&CA_A-GuG

2100~3 W O 92/13102 , PCTt~'S92/00340 -2~ 8 -(2) INFORMATION FOR SEQ ID NO: 400:
~i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 43 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D~ TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA3l (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 400:
CACCCATTTTGCCCAGAGCCTTCCTCTCCCACGTCTGCACATG
~2) INFORMATION FOR SEQ ID NO: 401:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: sing'e (D) TOPOLOGY: linear : (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) AN~I-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
: (B) CLONE: TGLA31 (repeat) ' ! (Xi) SEQUENCE DESCRIPTION: SEQ ID NO: 401:
CCCCCCCCCCCCCCCCGCCCCCC
~2) INFORMATION FOR SEQ ID NO: 402:
~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genorcc) (iii) HYPOTHETICAL: N5 (iv) ANTI-S~NS_: N~

WO 9~/13102 2 1 0 0 5 8 ~ PCl/US92/00340 --2~9--(vi) ORIGINA~ SOURCE:
~(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA31 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 402:
GGGTCACACAGGAGACAGAAGAATAAGCAGTGCCTGTGCAGGGCGGTGAC
CAGCAGCCAGGAGCC
~2) INFORMATION FOR SEQ ID NO: 403:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 75 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETIGAL: NO
~ ~ (iv) ANTI-SENSE: NO
- ~ (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA310 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 403:
TTGTTATTTCAGATCTGCCTCTATCAACCTTTTTTCTAGTTCAAGTTAhA
TTTTTCTAATCCTTTGCACATTTGA
(2) INFORMATION FOR SEQ ID NO: 404:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 37 base pairs (~ TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: ~os taurus (vii) IMMEDIATE SOURCE:
~8) CLONE: TGLA310 ~repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 404:
TGTGTGTGTGTGTG,GTGTG-G-GTGTGTGTuTGTG-~2) INFORMATION FOR SEQ ID NO: 4Q5:

W O 92/13102 , 2 1 0 0 S ~ 3 PCT/US9`2~00340 ~o--(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 92 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGY: li~ear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (Yii) IMMEDIATE SOURCE:
~B) CLONE: TGLA3l0 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 405:
TGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTT
GTAAAACGACGGCCAGTGAATTCACAAAATCTAGCACACCAA
(2) INFORMATION FOR SEQ ID NO: 406:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 51 base pairs ~: (B) TYPE: nucleic acid (C) STRANDEDNESS: single -~ ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
; (iv) ANTI-SENSE:` NO
:~ ~ (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~: (B) CLONE: TGLA3ll (up) ,~ ~ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 406:
TTGTTGCAAATGGCAAAATTTTGTTCTTTTTTATGGCTGAATA~TATTCT
A
(Z) INFORMATION FOR SEQ ID NO: 407:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l6 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii~ HYPOTHETICAL: N0 (iv) ANTI-SENSE: NO

WO 92/131~2 2 1 0 0 5 8 3 PCr/US92~00340 (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA311 ~repeat) txi) SEQUENCE DESCRIPTION: SEQ ID NO: 407:
GTGTGTGTGTGTGT&T
~2) INFORMATION FOR SEQ ID NO: 408:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 89 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic~
(iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 90s taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA311 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 408:
CAATGTTCATAGCAGTATTATTACAATTGCCAAGATACAGAAGCAACCTA
AGTGTCTATCAATGGATGATTAAATAAAAAGGATATGAT
(2) INFORMATION FOR SEQ ID NO: 40g:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 27 base pairs (B) TYPE: nucleic acid : tC) STRANDEDNESS: single (D) TOPOLOGY: linear , (ii) MOLBCULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
: (iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
~A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~) CLONE: TGLA318 (up) (xi) SEQUENCE DESCRIPTIO~: SEQ ID NO: 403:

AGCTCTTGACCTGTCTCGGCGGCAGGG
(2) INFORMATION FOR SEQ ID NO: 410:

2100~o3 -~
W O 92~13102 - PCT/US92/00340 (i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 36 base pairs (3) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear , I
(ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii~ IMMEDIATE SOURCE
(B) CLONE: TGLA318 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 410:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
~2) INFORMATION FOR SEQ ID NO: 411:
:~ (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 77 base pairs ~ (B) TYPE: nucleic acid :: (C) STRANDEDNESS: single (D) TOPOLOGY: linear ; (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
.~ (vi) ORIGINAL SOURCE:
-~ ~ (A) ORGANISM: Bos taurus (vii) IMMæDIATE SOURCE:
) CLONE: TGLA318 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 411:
TTAGGATATATCAGATGAGTAAGAATGCATTTACCCTTCACTATG-CTCC
, ; ATAGAAAGTTGCTTTGGGTTTGGAGGT
~ (2~ INFORMATION FOR SEQ ID NO: 412:
- ~i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 76 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:

WO ~?/13102 2 1 0 0 ~ 8 3 Pcr/US92!00340 _ ~93-(A) ORGANISM; Bos taurus (vii) IMMEDIATE SOURCE:
(3) CLONE: TGLA322 ~up) (xi~ SEQUENCE DESCRIPTION: SEQ ID NO: 412:
GGGACCTACATCTTCTGATGCTCTTTTCCTGTTCCCTCATGCCACCTCTT
GTCTGAAAGAGCAGTGTCATACAGGG
~2) INFORMATION FOR SEQ ID NO: 413:
(i) SEQUENCE CHARACTERISTICS:
~A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STR~NDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (~enomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(8) CLONE: TGLA322 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 413:
GTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 414:
~-(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 130 base pairs :(B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPO$HETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: 80s taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA322 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 414:
CTTTTGATGTAAAAATGTTCTGGAACTAGTTGGAGGTGACGGTGuCACAG
ACTTATAAATGTACTTGTAAATGCCAATGAATCATTCACTT-~ACATGG-TTAAATGACTATTTTATGTGGTATTTTAAT
(2) INFORMATION FOR SEQ ID NO: 415:

2101)~83 W O 92/~3102 PCT~US92tO0340 -29~ -(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 59 base pairs ~B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomlc) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA323 ~up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 415:
GCCAGGTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGG,~TGCGT
TTGTAAGTT
~2) INFORMATION FOR SEQ ID N0: 416:
(i) SEQUENCE CHARACTERISTICS:
~-~(A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single tD) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ~genomic) ,~
~-~(iii) HYPOTHETICAL: N0 iv) ANTI-SENSE: NO
(vi~ ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA323 (repeat) ~xi) SEQUENCE DESCRIPTI0N: SEQ ID N0: 416:
TGTGTGTGTGTGTGTGTG
~2~ INFORMATION FOR SEQ ID NO: 417:
:~i) SEQUENCE CHARACTERISTICS:
-(A) LENGTH: 96 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single (D3 TOPOLOGY: iinear (ii) MOLECULE TYPE: DNA (qenomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:

W O 92/13102 PCT/US9~/00340 21~0~83 -2~5 (A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA323 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 417:
AGCTCTTTGTAAGATTCAGGTCACTACAGAAAGAAACACTTNCTGTCAAA
CTACTCCATCATGCTGTCTGTTCATCCAGCCCCACCTTTTTCCCAT
(2) INFORMATION FOR SEQ ID NO: 418:
(i) SEQUENCE CHARACTE~ISTICS:
(A) LENGTH: 52 base pairs (B) TYPE: nucleic acid (C) STR,~NDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (qenomic) ~iii) HYPOTHETICAL: NO
~iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA325 (up) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 418:
CCAACACTGTGGGGCACTTTACTCTCTGAACA,~ATCTATAGTATTGGGGA
TT
~2) INFORMATION FOR SEQ ID NO: 419:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 45 base pairs ~B) TYPE: nucleic acid ¦ ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ' ~iii) HYPOTHETICAL: NO
¦ (iv) ANTI-SENSE: NO
I (vi) ORIGINAL SOURCE:
I (A) ORGANISM: Bos taurus i (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA325 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 419:
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG-GTG-(2) INFORMATION FOR SEQ ID NO: 420:
(i) SEQUENCE CHARACTER'STICS:

~lOOS$~
W O 92/l3102 PCT/US92~00340 -29~-- (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: single ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ~iii) HYPOTHETICAL: NO
(iv~ ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A~ ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B3 CLONE: TGLA325 (down) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 420:
AGCTGACAGTCTATTTCCAGAAGGTAAA
(2) INFORMATION FOR SEQ ID NO: 421:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 67 base pairs :~ (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) iii) HYPOTHETICAL: NO
~ (iv) ANTI-SENSE: NO
-~ (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA327 (up) ~; ~ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 421:
CCAGTGATCTGAAGGCTAAATAACTTCTCTATCTTGCCTTTAATAGTCTC
ACTGTGATACATAGGAT
j~ ~2) INFORMATION FOR SEQ ID NO: 422:
~ (i) SEQUENCE CHARACTERISTICS:
: (A) LENGTH: 25 base pairs , : (B) TYPE: nucleic acid ~ C) STRANDEDNESS: single :~ ~D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO

: ~iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANIS~: 3Os taurus W O 92/13102 2 1 0 Q ~ 8 3 PCT/US92/00340 - 2~_ (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA327 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 422:
TGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 423:
(i) SEQUENCE CHARACTERISTICS:
(A) ~ENGTH: 103 base pairs (B) T~PE: nucleic acid SC) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE~ DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA327 (down) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 423:
CCTGCTAATGCAGGAAGAGGTAAGAGACATGGGTTCGATCCCTGGGTTGG
GAAGNNNCCCCTGGAGGACGGCACAAAGACTCAGACATGACTGAAGAGAC
TTA
(2) INFORMATION FOR SEQ I~ NO: 424:
(i) SEQUENCE CHARACTERISTICS:
(A) LE~GTH: 146 base pairs (B) TYPE: nucleic acid (C) STRANDEDNFSS: single : (D) TOPOLOGY: linear tii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: ~NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(~) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~B) CLONE: TGLA328 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 424:
GAATTCCCTGCTTCAAATAAATCTTGTTTAAGGCAAATGGAAGG.AAGAG
AAAGGGCAGGAGGAGAGTAAAAGAAAGGCTGATATTGTAGCATCCTACCA
ATCTTTGTTTCTAAAT$GCAGCAGCCTTGAAAGAGAACAT5AA...
(2) INFORMATION FOR SEQ ID NO: 425:

W O 92/13102 - 2 1 ~ O ~ ~ 3 PCT/US92/00340 ~a-(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDE3~ESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
: (iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: ~os taurus : (vii) IMMEDIA.E SOUROE :
(B) CLONE: TGLA328 (repeat) ~xi) SEQUENCE DESCRIPTION: SEQ ID NO: 425:
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
(2) INFORMATION FOR SEQ ID NO: 426:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 131 base pairs ~: (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ (li) MOLECULE TYPE: DNA (genomic) :~ : (iii) HYPOTHETICAL: NO
, (iv) ANTI-SENSE: NO
~:~ (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (Yii) IMMEDIATE SOURCE:
~: (B) CLONE: TGLA328 (down) ~ xi) SEQUENCE DESCRI~TION: SEQ ID NO: 426:
:~ ~ jAGGCTCCAAGATATTGCCCTGAGTATAGGCAGCAGGTT~AAAAAAAGTTA
' CTCTCATTAATTATTTTTAGAAAGATGGTGACAAGATGGACAGTACCTAA
CTGTGAGATTGCTTTGTAGTAATAAGGCCTT
2) INFORMATION FOR SEQ ID NO: 427:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 46 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

WO 92/~3102 2 1 0 0 5 8 3 PCI/US92/00340 (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ; (vii) IMME~IATE SOURCE:
(B) CLONE: TGLA332 (up) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 427:
TGCTAAGTTGCTTCAGTCATGTCTGACTCTTTGCAACACCATGGAC
(2) INF9RMATION FOR SEQ ID NO: 428:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 34 base pairs (B) TYPE: nucleic acid ~C) STRANDEDNESS: si~gle (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYP9T~ETICAL: NO
(iv~ ANTI-SENSE: NO.
:: ^
: (vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
M ~-: : (B) CLONE: TGLA332 (repeat) .,.,,~, ~
~:: (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 428:
TGTGTGTGTGTGTGTGTGTGTTATGTGTGCTGTG
2) I~FORMATION FOR SEQ ID NO: 429:
SEQUENCE CHARACTERISTICS:
:: (A) LENGTH: 24 base pairs . ~ ~ (B) TYPE: nucIeic acid i~ : ~C) S RANDEDNESS: single (D) TOPOLOGY: linear -~ ~ (ii) MOLECULE TYPE: DNA (genomic) ' ~ ,,, ! (iii) HYPOTHETICAL: :NO
- :. (iv) ANTI-SENSE: NO
-., -:
-~ (vi) ORIGINAL SOURCE:
:~: (A) ORGANISM: 30s taurus ~ vii) IMMEDIATE SOURCE:
i- ~B) CLONE: TGLA332 (down) - (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 429:

GTCAGACATAACTGAGTGACTAAG
(2) INFORMATION FOR SEQ ID NO: 430:
(i) SEQUENCE CHARAC~ERISTICS:
(A) LENGTH: 89 base pa~rs W O 92~l3102 , - 2 1 ~ O ~ ~ 3 PCT~US92/00340 `

_ 3OO-(B) TYPE: nucleic acid (C) STRANDEDNESS: single ~D) TOPOLOGV: linear (ii) MOLECULE TYPE: DNA (genomic) (iii~ HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
~vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus ~vii) IMMEDIATE SOURCE:
(B) CLONE: TGLA3 3 4 ( up ) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 430:
GGGCGAGCTCGAATTCCCTCGTCAGTGGAGAAATGGGATTCAACTCAGGT
AGTCCAATTCCAATGCTTTAATGACTTGTTTCTAAATGC
(2) INFORMATION FOR SEQ ID NO: 431:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO
(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus (vii) IMMEDIATE SOURCE:
~: (B) CLONE: TGLA334 (repeat) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 431:
TTTTTTTTTTTT
(2) INFORMATION FOR SEQ ID NO: 432:
(i) SEQUENCE CHARACTERISTICS:
~r (A) LENGTH: 65 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO
(iv) ANTI-SENSE: NO

(vi) ORIGINAL SOURCE:
(A) ORGANISM: Bos taurus . ~

DEMANDES OU BREVETS \/OLUI\IIII\~EUX

LA PRÉSE~JTE PARTIE DE CErrE DE~VIANDE OIJ CE BREVET
COMPR ND PLUS D'UN TOME. I

CECI EST LE TC)ME ~ DE_ -, .~ , ,' ,, .

. NOTE: POUr IQS tome~ addi~ionels, Yeuiltez contacter le E3ureau canadien des .
brevets .~l ~ 5 ~ 3 5 _ __ _ _ . . ..
: ~ JUME30 APPLICATIONS/PATENTS

. ' _ ~
~: ~ THIS SE~TION OF THE APPLICATION/PATENT CONTA~NS MORE
I THAN O E VOLUME

THIS IS VOLUME ~ OF _ L~ additional voiume~ plea~ contact the Canadian Patent Oftice

Claims (25)

WHAT IS CLAIMED IS:

1. A set of nucleic acid fragments that hybridize to polymorphic loci in bovids, wherein said set com-prises fragments that hybridize to at least five unique loci and each fragment hybridizes to a locus comprising at least two alleles and with a hetero-zygosity of at least 50%.

2. The set of claim 1, wherein said polymorphic loci are selected from the group consisting of VNTR
loci, multisite haplotype loci, microsatellite loci and combinations thereof.

3. The set of claim 2, wherein said polymorphic loci are VNTR loci.

4. The set of claim 3, wherein said fragments are selected from the group of VNTR markers identified in Table 1.

5. The set of claim 2, wherein said polymorphic loci are multisite haplotype loci.

6. The set of claim 5, wherein said fragments are selected from the group of multisite haplotype markers identified in Table 5.

7. The set of claim 2, wherein said polymorphic loci are microsatellite loci.

8. The set of claim 7, wherein said fragments are selected from the group of microsatellite markers identified in Table 7.

9. The set of claim 1, wherein said bovids are bovines.

10. The set of claim 9, wherein said bovines are of the species Bos taurus.

11. The set of claim 1, wherein said bovids are ovines.

12. The set of claim 1, wherein said fragments are obtained from a bovid genome.

13. The set of claim 12, wherein said bovid is a bovine.

14. The set of claim 13, wherein said bovine is of the species Bos taurus.

15. The set of claim 2, wherein said fragments are selected from the group of VNTR markers identified in Table 1, the group of multisite haplotype markers identified in Table 5, the group of micro-satellite markers identified in Table 7, and combinations thereof.

16. The set of claim 7, wherein said fragments are selected from the group of microsatellite markers identified in Table 8.

17. A synteny map of microsatellite markers identified in Table 9.

18. A synteny map of VNTR markers identified in Table 4.

19. The microsatellite marker TGLA116 for the Weaver condition.

20. The microsatellite marker TGLA116 for the QTL trait of enhanced milk production in Brown Swiss cattle.

21. A set of nucleic acid fragments comprising at least one fragment selected from the group consisting of the VNTR markers identified in Table 1, the multi-site haplotype markers identified in Table 5, and the microsatellite markers identified in Table 7.

22. A process for mapping quantitative traits in bovids which comprises using the set of claim 21.

23. A process for genetic identification using the set of claim 21.

24. A process for introducing a desired gene into a bovid which comprises using the set of claim 21.

25. The process of claim 21, which further comprises the use of velogenesis.