CA2098331A1 - Method for the isolation of degradative microbial consortia and apparatus therefore - Google Patents

Method for the isolation of degradative microbial consortia and apparatus therefore

Info

Publication number
CA2098331A1
CA2098331A1 CA 2098331 CA2098331A CA2098331A1 CA 2098331 A1 CA2098331 A1 CA 2098331A1 CA 2098331 CA2098331 CA 2098331 CA 2098331 A CA2098331 A CA 2098331A CA 2098331 A1 CA2098331 A1 CA 2098331A1
Authority
CA
Canada
Prior art keywords
microorganisms
isolation
contaminant
microbial consortia
metabolizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2098331
Other languages
French (fr)
Other versions
CA2098331C (en
Inventor
John R. Lawrence
M. Jim Hendry
Gideon M. Wolfaardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canada Minister of Environment
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 2098331 priority Critical patent/CA2098331C/en
Publication of CA2098331A1 publication Critical patent/CA2098331A1/en
Application granted granted Critical
Publication of CA2098331C publication Critical patent/CA2098331C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

There is disclosed a method for isolating microorganisms which are capable of metabolizing a selected contaminant at a variety of concentrations. An apparatus in the form of an extended concentration gradient gel cell is disclosed for the purpose of sustaining the contaminant in various concentration levels on the gel. The microorganisms self colonize on the gel cell at a concentration suitable for metabolic activity of the microorganisms. This permits easy identification and isolation of microorganisms capable of metabolizing various concentrations of a contaminant.
CA 2098331 1993-06-14 1993-06-14 Method for the isolation of degradative microbial consortia and apparatus therefore Expired - Fee Related CA2098331C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2098331 CA2098331C (en) 1993-06-14 1993-06-14 Method for the isolation of degradative microbial consortia and apparatus therefore

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2098331 CA2098331C (en) 1993-06-14 1993-06-14 Method for the isolation of degradative microbial consortia and apparatus therefore

Publications (2)

Publication Number Publication Date
CA2098331A1 true CA2098331A1 (en) 1994-12-15
CA2098331C CA2098331C (en) 2001-08-21

Family

ID=4151788

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2098331 Expired - Fee Related CA2098331C (en) 1993-06-14 1993-06-14 Method for the isolation of degradative microbial consortia and apparatus therefore

Country Status (1)

Country Link
CA (1) CA2098331C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3081633A1 (en) * 2015-04-16 2016-10-19 ARKRAY, Inc. Device and system for cell culture
WO2024084460A1 (en) * 2022-10-21 2024-04-25 Janssen Research & Development, Llc Methods for microbial detection

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3081633A1 (en) * 2015-04-16 2016-10-19 ARKRAY, Inc. Device and system for cell culture
CN106047690A (en) * 2015-04-16 2016-10-26 爱科来株式会社 Cell culture device
WO2024084460A1 (en) * 2022-10-21 2024-04-25 Janssen Research & Development, Llc Methods for microbial detection

Also Published As

Publication number Publication date
CA2098331C (en) 2001-08-21

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Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed