CA2089495C - Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima - Google Patents

Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima Download PDF

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Publication number
CA2089495C
CA2089495C CA002089495A CA2089495A CA2089495C CA 2089495 C CA2089495 C CA 2089495C CA 002089495 A CA002089495 A CA 002089495A CA 2089495 A CA2089495 A CA 2089495A CA 2089495 C CA2089495 C CA 2089495C
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CA
Canada
Prior art keywords
dna
dna polymerase
sequence
polymerise
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002089495A
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English (en)
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CA2089495A1 (fr
Inventor
David H. Gelfand
Frances C. Lawyer
Susanne Stoffel
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication date
Priority claimed from US07/567,244 external-priority patent/US5374553A/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2089495A1 publication Critical patent/CA2089495A1/fr
Application granted granted Critical
Publication of CA2089495C publication Critical patent/CA2089495C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002089495A 1990-08-13 1991-08-13 Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima Expired - Lifetime CA2089495C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/567,244 US5374553A (en) 1986-08-22 1990-08-13 DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US567,244 1990-08-13
PCT/US1991/005753 WO1992003556A1 (fr) 1990-08-13 1991-08-13 ENZYME D'ACIDE NUCLEIQUE THERMOSTABLE PURIFIEE PROVENANT DE L'EUBACTERIE $i(THERMOTOGA MARITIMA)

Publications (2)

Publication Number Publication Date
CA2089495A1 CA2089495A1 (fr) 1992-02-14
CA2089495C true CA2089495C (fr) 2007-04-03

Family

ID=37951652

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002089495A Expired - Lifetime CA2089495C (fr) 1990-08-13 1991-08-13 Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima

Country Status (1)

Country Link
CA (1) CA2089495C (fr)

Also Published As

Publication number Publication date
CA2089495A1 (fr) 1992-02-14

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