CA2072231A1 - Determination of genetic sex in fish - Google Patents

Determination of genetic sex in fish

Info

Publication number
CA2072231A1
CA2072231A1 CA 2072231 CA2072231A CA2072231A1 CA 2072231 A1 CA2072231 A1 CA 2072231A1 CA 2072231 CA2072231 CA 2072231 CA 2072231 A CA2072231 A CA 2072231A CA 2072231 A1 CA2072231 A1 CA 2072231A1
Authority
CA
Canada
Prior art keywords
sex
dna
fish
provides
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2072231
Other languages
French (fr)
Other versions
CA2072231C (en
Inventor
Robert H. Devlin
Edward M. Donaldson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canada Minister of Fisheries and Oceans
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 2072231 priority Critical patent/CA2072231C/en
Publication of CA2072231A1 publication Critical patent/CA2072231A1/en
Application granted granted Critical
Publication of CA2072231C publication Critical patent/CA2072231C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention provides a method of isolating sex specific DNA sequences in fish that includes the steps of hybridizing an excess of denatured, sheared DNA of a first sex with a lesser amount of denatured DNA of a second sex that has been digested with a restriction endonuclease that produces sticky ends, and ligating resulting double-stranded fragments having two sticky ends derived from the DNA of the second sex into a cloning vector. This invention also provides DNA probes isolated by the preceding method. This invention also provides methods of identifying sex specific DNA
sequences using the aforementioned probes or, by using PCR primers derived from the aforementioned probes.
CA 2072231 1992-06-24 1992-06-24 Determination of genetic sex in fish Expired - Fee Related CA2072231C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2072231 CA2072231C (en) 1992-06-24 1992-06-24 Determination of genetic sex in fish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2072231 CA2072231C (en) 1992-06-24 1992-06-24 Determination of genetic sex in fish

Publications (2)

Publication Number Publication Date
CA2072231A1 true CA2072231A1 (en) 1993-12-25
CA2072231C CA2072231C (en) 2005-05-10

Family

ID=4150065

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2072231 Expired - Fee Related CA2072231C (en) 1992-06-24 1992-06-24 Determination of genetic sex in fish

Country Status (1)

Country Link
CA (1) CA2072231C (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759780A (en) * 1996-03-29 1998-06-02 Pathogenesis Corporation Methods for enriching target nucleic acid sequences
US9651565B2 (en) 2010-11-29 2017-05-16 Mote Marine Laboratory, Inc. Fish sexual characteristic determination using peptide hormones
CN114717302A (en) * 2022-04-08 2022-07-08 中国水产科学研究院黑龙江水产研究所 Molecular marker, kit and method for distinguishing genders of triangular breams
CN116287174A (en) * 2023-04-17 2023-06-23 中国海洋大学 DNA molecular marker related to genetic sex of Japanese scallop and identification method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759780A (en) * 1996-03-29 1998-06-02 Pathogenesis Corporation Methods for enriching target nucleic acid sequences
US9651565B2 (en) 2010-11-29 2017-05-16 Mote Marine Laboratory, Inc. Fish sexual characteristic determination using peptide hormones
US10620224B2 (en) 2010-11-29 2020-04-14 Mote Marine Laboratory, Inc. Fish sexual characteristic determination using peptide hormones
CN114717302A (en) * 2022-04-08 2022-07-08 中国水产科学研究院黑龙江水产研究所 Molecular marker, kit and method for distinguishing genders of triangular breams
CN116287174A (en) * 2023-04-17 2023-06-23 中国海洋大学 DNA molecular marker related to genetic sex of Japanese scallop and identification method
CN116287174B (en) * 2023-04-17 2023-10-10 中国海洋大学 DNA molecular marker related to genetic sex of Japanese scallop and identification method

Also Published As

Publication number Publication date
CA2072231C (en) 2005-05-10

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