CA2064815C

CA2064815C - Therapeutic heterocyclic compounds

Info

Publication number: CA2064815C
Application number: CA002064815A
Authority: CA
Inventors: Alan Duncan Robertson; Alan Peter Hill; Robert Charles Glen; Graeme Richard Martin
Original assignee: Wellcome Foundation Ltd
Current assignee: AstraZeneca AB
Priority date: 1990-06-07
Filing date: 1991-06-06
Publication date: 1999-11-16
Anticipated expiration: 2011-06-06
Also published as: SI21560A; IE911931A1; NZ238424A; US5863935A; LV10274B; PT97888B; JPH05502679A; CA2282890A1; FI20001406A; SI9111010A; SI9111010B; DE69127260D1; FI105686B; LT3264B; IL98392A0; EP0636623A1; DE69127260T2; IL114690A; EP0486666A1; IL114690A0

Abstract

The present invention is concerned with compounds of formula (I), wherein n is an integer of from 0 to 3; W is a group of formula (i), (ii), or (iii), wherein R is hydrogen or C1-4 alkyl, X is -O-, -S-, -NH-, or -CH2-, Y is oxygen or sulphur and the chiral centre (*) in formula (i) or (ii) is in its (S) or (R) form or is a mixture thereof in any proportions; and Z is a group of formula (iv), (v), or (vi), wherein R1 and R2 are independently selected from hydrogen and C1-4 alkyl and R3 is hydrogen or C1-4 alkyl; and their salts, solvates and physiologically functional derivatives, with processes for their preparation, with medicaments containing them and with their use as therapeutic agents, particularly in the prophylaxis and treatment of migraine.

Description

Therapeutic Heterocyclic Compounds The present invention is concerned with new chemical compounds, their preparation, pharmaceutical formulations containing them and their use in medicine, particularly the prophylaxis and treatment of migraine.
Receptors which mediate the actions of 5-hydroxytryptamine (5-HT) have been identified in mammals in both the periphery and the brain. According to the classification and nomenclature proposed in a recent article (Bradley et al, Neuropharmac., 25, 563 (1986)), these receptors may be classified into three main types, viz. "5-HT1-like", 5-HT2 and 5-HT3. Various classes of compounds have been proposed as 5-HT agonists or antagonists for therapeutic use, but these have not always been specific to a particular type of 5-HT
receptor. European Patent Specification 0313397 describes a class of 5-HT agonists which are specific to a particular type of "5-HT1-like" receptor and are effective therapeutic agents for the treatment of clinical conditions in which a selective agonist for this type of receptor is indicated. For example, the receptor in question mediates vasoconstriction in the carotid vascular bed and thereby modifies blood flow therein.
The compounds described in the European Specification are therefore beneficial in the treatment or prophylaxis of conditions wherein vasoconstriction in the carotid vascular bed is indicated, for example, migraine, a condition associated with excessive dilation of the carotid vasculature. However, it is within the scope of the earlier application that the target tissue may be any tissue wherein action is mediated by "5-HT1-like"
receptors of the type referred to above.
We have now found a further class of compounds having exceptional "5-HT1-like" receptor agonism and excellent absorption following oral dosing. These properties render the compounds particularly useful for certain medical applications, notably the prophylaxis and .
treatment of migraine, cluster headache and headache associated with vascular disorders, hereinafter referred to collectively as "migraine".
According to the first aspect of the present invention, therefore, there is provided a compound of formula (I) H
cz) ~ (CH ) 2n Z
wherein n is 1, W is a group of formula:

Y
NR
X J-wherein R is hydrogen; X is -O-; Y is oxygen; and the chiral centre * in W is in its (S) or (R) form or is a mixture thereof in any proportions; and Z is a group of formula:

wherein R1 and R2 are each methyl; and physiologically acceptable salts and solvates; and physiologically functional derivatives thereof.
Thus the compound of formula (I) having exceptional properties for the treatment and prophylaxis of migraine is N,N-dimethyl-2-[5-(2-oxo-2,3-oxazolidin-4-yl-methyl)-1H-indol-3-yl]ethylamine in either its (S) or (R) form or as a mixture thereof in any proportions. The salts and solvates of this compound, for example, the hydrate maleates, are particularly preferred.
Physiologically acceptable salts are particularly suitable for medical applications because of their greater aqueous solubility relative to the parent, i.e., basic compounds. Such salts must clearly have a physiologically acceptable anion. Suitable physiologically acceptable salts of the compounds of the present invention include those derived from acetic, hydrochloric, hydrobromic, phosphoric, malic, malefic, fumaric, citric, sulphuric, lactic or tartaric acid. The succinate and chloride salts are particularly preferred for medical purposes. Salts having a non-physiologically acceptable anion are within the scope of the invention as useful intermediates for the preparation of physiologically acceptable salts and/or for use in non-therapeutic, for example in vitro, situations.
According to a second aspect of the present invention, there is provided a compound of formula (I) or a physiologically acceptable salt, solvate or physiologically functional derivative thereof for use as a therapeutic agent, specifically as a "5-HT1-like"
receptor agonist, for example, as a carotid vasoconstrictor in the prophylaxis and treatment of migraine. As indicated, however, target organs for the present compounds other than the carotid vasculature are within the scope of the present invention.
The amount of a compound of formula (I), or a salt or solvate thereof, which is required to achieve the desired biological effect will depend on a number of factors such as the specific compound, the use for which it is intended, the means of administration, and the recipient.
A typical daily dose for the treatment of migraine may be expected to lie in the range 0.01 to 5mg per kilogram -S-body weight. Unit doses may contain from 1 to 100mg of a compound of formula (I), for example, ampoules for injection may contain from 1 to lOmg and orally administrable unit dose formulations such as tablets or capsules may contain from 1 to 100mg. Such unit doses may be administered one or more times a day, separately or in multiples thereof. An intravenous dose may be expected to lie in the range 0:01 to 0.15mg/kg and would typically be administered as an infusion of from 0.0003 to 0.15mg per kilogram per minute. Infusion solutions suitable for this purpose may contain from 0.01 to lOmg/ml.
when the active compound is a salt or solvate of a compound of formula (I), the dose is based on the cation (for salts) or the unsolvated compound.
Hereinafter references to "compound(s) of formula (I)"
will be understood to include physiologically acceptable salts and solvates thereof.
According to a third aspect .of the present invention, therefore, there are provided pharmaceutical compositions comprising, as active ingredient, at least one compound of formula (I) and/or a pharmacologically acceptable salt or solvate thereof together with at least one pharmaceutical carrier or excipient. These pharmaceutical compositions may be used in the prophylaxis or treatment of clinical conditions for which a "5-HT1-like" receptor agonist is indicated, for example, migraine. The carrier must be pharmaceutically acceptable to the recipient and must be compatible with, i.e., not have a deleterious effect upon the other ingredients in the composition. The carrier may be a solid or liquid and is preferably formulated with at least one compound of formula (I) as a unit dose formulation, for example, a tablet which may contain from 0.05 to 95% by weight of the active ingredient. If desired, other physiologically active ingredients may also be incorporated in the pharmaceutical compositions of the invention.
Possible formulations include those suitable for oral, sublingual, buccal, parenteral (for example, subcutaneous, intramuscular, or intravenous), rectal, topical and intranasal administration. The most suitable means of administration for a particular patient will depend on the nature and severity of the condition being treated and on the nature of the active compound, but, where possible, oral administration is preferred.
Formulations suitable for oral administration may be provided as discrete units, such as tablets, capsules, cachets or lozenges, each containing a predetermined amount of the active compound; as powders or granules; as solutions or suspensions in aqueous or non-aqueous liquids; or as oil-in-water or water-in-oil emulsions.
Formulations suitable for sublingual or buccal administration include lozenges comprising the active compound and, typically, a flavoured base, such as sugar and acacia or tragacanth and pastilles comprising the _7_ active compound in an inert base, such as gelatin and glycerin or sucrose and acacia.
Formulations suitable for parenteral administration typically comprise sterile aqueous solutions containing a predetermined concentration of the active compound; the solution is preferably isotonic with the blood of the intended recipient. Although such solutions are preferably administered intravenously, they may also be administered by subcutaneous or intramuscular injection.
Formulations suitable for rectal administration are preferably provided as unit-dose suppositories comprising the active ingredient and one or more solid carriers forming the suppository base, for example, cocoa butter.
Formulations suitable for topical or intranasal application include ointments, creams, lotions, pastes, gels, sprays, aerosols and oils. Suitable carriers for such formulations include petroleum jelly, lanolin, polyethylene glycols, alcohols and combinations thereof.
The active ingredient is typically present in such formulations at a concentration of from 0.1 to 15% w/w.
The formulations of the invention may be prepared by any suitable method, typically by uniformly and intimately admixing the active compounds) with liquids or finely divided solid carriers, or both, in the required proportions and then, if necessary, shaping the resulting mixture into the desired shape.

_g_ For example, a tablet may be prepared by compressing an intimate mixture comprising a powder or granules of the active ingredient and one or more optional ingredients, such as a binder, lubricant, inert diluent, or surface active dispersing agent, or by moulding an intimate mixture of powdered active ingredient and inert liquid diluent.
Aqueous solutions for parenteral administration are typically prepared by dissolving the active compound in sufficient water to give the desired concentration and then rendering the resulting solution sterile and isotonic.
Thus, according to a fourth aspect of the present invention, there is provided the use of a compound of formula (I) in the preparation of a medicament for the prophylaxis or treatment of a clinical condition for which a "5-HT1-like" receptor agonist is indicated, for example, migraine.
According to a fifth aspect, there is provided a method for the prophylaxis or treatment of a clinical condition in a mammal, for example, a human, for which a "5-HT1-like" receptor agonist is indicated, for example, migraine, which comprises the administration to said mammal of a therapeutically effective amount of a compound of formula (I) or a physiologically acceptable salt, solvate or physiologically functional derivative thereof .

According to a sixth aspect of the invention, compounds of formula (I) may be prepared by reacting a compound of formula (II) (isolated or in situ - infra).

(II) W
~ (CH ) 2n wherein n and W are as hereinbefore defined, with a compound of formula (III) H
L (III) or a carbonyl-protected form thereof, such as the dimethyl or diethyl acetal, wherein L is a suitable leaving group, such as chlorine, or a protected amino group, either of which may be converted in situ to an - 1~ -amino group, or is -NRlRz where Rl and RZ are as hereinbefore defined. The reaction is typically carried out by refluxing the compounds in a polar solvent system, for example, ethanol/water, dilute acetic acid or water in the presence of an acidic ion exchange resin, for example, "Amberlyst 15" (Trade-mark).
Standard N-methylation methods may be used to convert compounds of formula (I) wherein R1 and/or RZ are hydrogen to corresponding compounds (I) wherein R1 and Rz are methyl.
Compounds of formula (I) may be prepared from the corresponding compound wherein R1 - Rz - H by methods of N,N-dimethylation well known to those skilled in the art, for example, by treatment with the appropriate aldehyde in the presence of a reducing system, for example, sodium cyanoborohydride/-acetic acid, in a polar solvent, such as methanol.
Compounds of formula (I) wherein R1 or Rz is methyl and the other is hydroxy may. be prepared from the corresponding compound wherein R1 - R2 - H by N-benzylation using benzaldehyde and a suitable reducing agent, for example, sodium borohydride, in a polar solvent, such as ethanol, followed by N-methylation using a suitable agent, such as the appropriate methyl sulphate, typically in the presence of a base, for example, anhy, potassium carbonate, in a polar aprotic solvent, such as DMF, and finally N-debenzylation, typically by catalytic hydrogenation using, for example, Pd/C in a polar solvent, such as ethanol.
Hydrazines of formula (II). may be prepared from the corresponding aniline of formula (IV) NH
(IV) CH
2~n wherein n and W are as hereinbefore defined, by diazotisation followed by reduction. Diazotisation is typically carried out using sodium nitrite/c.HCl and the resulting diazo product reduced in situ using, for example, tin(II) chloride/c.HCl. The resulting hydrazine may be isolated or converted to a compound of formula (I) in situ.
Anilines of formula (IV) may be prepared by reduction of the corresponding p,-nitro compound of formula (V) . ~ N02 (v) CH
2)n wherein n and W are as hereinbefore defined, typically by catalytic hydrogenation using, for example, Pd/C in a polar solvent system, such as an acidified mixture of ethanol, water and ethyl acetate.
Anilines of formula (IV) may also be prepared by cyclising a compound of formula (XXXIII) H(R4)N ~ NH2 HX (XXXI I I ) (CH2)n wherein n and X are as hereinbefore defined and R4 is -COZRS where RS is C1_4 alkyl, typically by heating in the presence of a base, such as sodium methoxide.
Compounds of formula (XXXIII) may be prepared by reducing a corresponding C1_4 alkyl ester using, for example, sodium borohydride, in a polar solvent system, such as ethanol/water, at 0°C. The ester, may be prepared by esterifying the corresponding carboxylic acid using, for example, the appropriate alcohol and HC1 or by reducing the corresponding p-nitro compound, for example, by catalytic hydrogenation. Both the acid and the p,-nitro compound may be prepared from the corresponding g-nitroaminoacid, the acid by N-alkoxycarbonylation using, for example, RSOCOCl where RS is as hereinbefore def fined, followed by reduction of the nitro group, for example, by catalytic hydrogenation, or by reduction of the nitro group followed by N-alkoxycarbonylation, and the g-nitro compound by N-alkoxycarbonylation (as for the acid) followed by esterification using, for example, the appropriate alcohol and HC1, or by esterification followed by N-alkoxycarbonylation. The g-nitroaminoacid may be obtained commercially or prepared from readily available starting materials by methods known to those skilled in the art or obtainable from the chemical literature, for example, by p,-nitration of the corresponding aminoacid using, for example, c.H2S02/c.HN03 at 0°C .
p-Nitro compounds of formula (V) may be prepared by reacting a compound of formula (VI) H(R)N . ~ N~2 HX (vI ) (CH ) 2n wherein n, R and X are as hereinbefore defined, with a compound of formula (VII) / L (VII) Y =C~
L' wherein Y is as hereinbefore def fined and L and L' , which may be the same or different, are suitable leaving groups, for example, chlorine, ethoxy, trichloromethyl, trichloromethoxy, or imidazoyl, for example, in the case where L - L' - chlorine, in a non-polar solvent, such as toluene, in the presence of a base, for example, potassium hydroxide.

- IS -Compounds of formula (VI) may be prepared by ring-opening a compound of formula (V), for example, by refluxing in 2N aqu. KOH.
Compounds of formula (VI) may be prepared by esterification of the corresponding carboxylic acid, typically by treatment with thionyl chloride and an appropriate alcohol at -10°C. followed by reduction of the ester using, for example, sodium borohydride, in a polar solvent system, such as ethanol/water, at 0°C. The acid may be obtained commercially or prepared from readily available starting materials by methods known to those skilled in the art or obtainable from the chemical literature, for example, by p-nitration of the corresponding aminoacid using, for example, c.H2S04/c.HN03 at 0°C .
Compounds of formula (III) and (VII) may be obtained commercially or prepared from readily available starting materials by methods known to those skilled in the art or obtainable from the chemical literature.
p-Nitro compounds of formula (V) may also be prepared by g-nitration of a compound of formula (XXXVI) (XXXVI ) CH ~ .
2~n wherein n and W are as hereinbefore defined using, for example, c.H2S02/c.HN03 at 0°C.
Compounds of formula (XXXVI) may be prepared by reacting a compound of formula (XXXVII) H(R)N
HX (XXXVII) (CH ) 2n wherein n, R and X are as hereinbefore defined, with a compound of formula (VII) wherein Y, L and L' are as hereinbefore defined, typically in the presence of a base, for example, potassium hydroxide in a non-polar solvent, such as toluene.
Compounds of formula (XXXVII) may be prepared by reducing the corresponding nitro compounds, typically by catalytic hydrogenation using, for example, Pd/C in a polar solvent, such as ethanol. The nitro compound corresponding to the compound of formula (XXXVII) may be prepared by reacting a compound of formula (XXIV) (XXIV) 2 n+1 wherein n is as hereinbefore defined, with paraformaldehyde in a polar aprotic solvent, such as DMF, in the presence of a base, for example, sodium methoxide, at 0°C, or by esterification of the corresponding carboxylic acid, 'typically by treatment with thionyl chloride and an appropriate alcohol at -10°C., followed by reduction of the ester group using, for example, sodium borohydride, in a polar solvent system, such as ethanol/water, at 0°C.
The compound of formula (XXIV), the acid and the aldehyde may be obtained commercially or prepared from readily available starting materials by methods known to those skilled in the art or obtainable from the chemical literature.
Compounds of formula (I) may also be prepared by reacting a compound of formula (XV) wherein n, R, X and Z are as hereinbefore defined, with a compound of formula (VII) wherein Y, L and L' are as hereinbefore defined, for example, in the case where L = L' = ethoxy, by heating in the presence of a base, for example, potassium carbonate.

Compounds of formula (XV) may be prepared by ring-opening a compound of formula (I) wherein n, Z and W are as hereinbefore defined in which R, X and Y are as hereinbefore defined, for example, by refluxing in 2N
aqu. KOH.

Compounds of formula (XV) may be prepared by esterification of the corresponding carboxylic acid, typically by treatment with thionyl chloride and an appropriate alcohol at -10°C., followed by reduction of the ester using, for example, sodium borohydride, in a polar solvent system, such as ethanol/water, at 0°C. The acid may be prepared by ring-opening a compound of formula (XVI) H
NR ~ N
XVI
( >
R N
(CH2)n .
Z
O
wherein n, R and Z are as hereinbefore defined and R6 is hydrogen or benzyl, typically by refluxing in water in the presence of a base, for example, barium hydroxide.
Compounds of formula (XVI ) wherein n = 1 may be prepared by reducing a compound of formula (XVII) H
R
O N / CH ~ (XVI I ) (CH
R6 2)n_1 Z
N
O
wherein n, R, R6 and Z are as hereinbefore defined, typically by catalytic hydrogenating using, for example, Pd/C in a polar solvent system, such as ethanol/water.

Alternatively, an enantioselective reducing agent, such as Rh(cod)(dipamp) -BF2 (JCS. Chem. Comm. 275 (1991)), may be used to reduce the double bond and thereby introduce a chiral centre at the 4-position of the dioxoimidazole ring.
Compounds of formula (XVII) may be prepared by reacting a compound of formula (XVIII) H
(xvIII) OHC ~
{CH2)n-1 Z

wherein n and Z are as hereinbefore defined, with, in the case where R6 is to be hydrogen, a compound of formula (X) wherein R is as hereinbefore defined, typically by heating in glac. acetic acid in the presence of ammonium acetate.
Compounds of formula (XVIII) may -be prepared by the reduction hydrolysis of the corresponding nitrile, typically using Raney nickel and sodium hypophosphite in a mixture of water, acetic acid and pyridine.

Compounds of formula (XVI) wherein R6 is benzyl may be prepared by reacting a compound of formula (XXXV) NR ~ NHNH2 (xxxv) Bz ~ (CH2)~
O
wherein n and R are as hereinbefore defined, with a compound of formula (III) wherein L is as hereinbefore defined, typically using the reaction conditions described above for the reaction of (II) with (III).
Hydrazines of formula (XXXV) may be prepared from the corresponding aniline, typically using the reaction conditions described above for the conversion of (IV) to (II). The aniline may be prepared by reducing the corresponding g-nitro compound, typically using the reaction conditions described above for the conversion of (V) to (IV). The g-nitro compound may be prepared by reacting the corresponding p-nitroaminoacid with benzyl isocyanate in the presence of base, for example, potassium hydroxide, in a polar solvent, such as water.
The p-nitroaminoacid may be obtained commercially or prepared from readily available starting materials by methods known to those skilled in the art or obtainable from the chemical literature, for example, by ~-nitration of the corresponding aminoacid using, for example, c . H2S04/c . HN03 at 0°C .
Compounds of formula (XV) may be prepared by reducing a compound of formula (XX) H
02N ~ N .
(xx) HX
(CH2)n Z
wherein n, X and Z are as hereinbefore defined, typically by catalytic hydrogenation using, for example, Pd/C in a polar solvent, such as ethanol.
Compounds of formula (XX) may be prepared by reacting a compound of (XXI ) H
N
02N w (CH2)n+1 Z (xxI) wherein n and Z are as hereinbefore defined, with paraformaldehyde in a polar aprotic solvent, such as DMF, in the presence of a base, fo,r example, sodium methoxide, at 0°C .
Compounds of formula (XXI) may be prepared by reacting a compound of formula (XXII) H
(xxll) ~2N ~ CH
2~n+1 wherein n is as hereinbefore defined, with, the appropriate compound of formula (XXVIII) wherein R3 is as hereinbefore defined, typically by heating in glac.
acetic acid.
Compounds of formula (XXII) wherein n = 1 may be prepared by reducing a compound of formula (XXIII) H
N (XXIII) ~2N~CH iCH~
(CH2)n-1 wherein n is as hereinbefore defined, using, for example, sodium borohydride and 40% w/v aqu. NaOH in a polar aprotic solvent such as acetonitrile, at 0°C.
Compounds of formula (XXIII) may be prepared by heating the appropriate aldehyde with nitromethane in the presence of ammonium acetate. The aldehyde may be prepared from a compound of formula (XIX) wherein n is as hereinbefore defined using the reaction conditions described above for preparing a compound of formula (XVIII) from the corresponding nitrile.
Compounds of formula (XXII) where n - 1 may be obtained commercially or prepared from readily available starting materials by methods known to those skilled in the art or obtainable from the chemical literature.
Compounds of formula (XXI) wherein n - 1 may also be prepared from a compound of formula (XXXIX) H
. ~ N
(XXXIX) ~2N ~ CH ~ CH ~ CH
2~n-1 Z
wherein n and Z are as hereinbefore defined, using reaction conditions analogous to those used to convert (XXIII) to (XXII). Compounds of formula (XXXIX) may be prepared from a compound of formula (XVIII) wherein n and Z are as hereinbefore defined using reaction conditions analogous to those used to prepare (XXIII) from the appropriate aldehyde and nitromethane.
Compounds of formula (I) may also be prepared from a compound of formula (XXXI) H
N
W ' / (xxxl ) (CH2)n wherein n and W are as hereinbefore defined, by methods known to those skilled in the art or obtainable from the chemical literature, for example, by treatment with (COL)2, where L is a suitable leaving group, for example, chlorine, to give the corresponding 3-COCOL compound which may then be treated with HNR1R2, where R1 and Rz are as hereinbefore defined, and reduced using, for example, lithium aluminium hydride. Alternatively, the compound of formula~(XXXI) may be treated with CH20/KCN to give the corresponding 3-cyanomethyl compound which may then be catalytically hydrogenated over Raney nickel in the presence of NHR1R2 as hereinbefore defined.
The aforementioned 3-cyanomethyl compound may also be prepared by cyclising a compound of formula (XXXX) I NHN = CH(CH2)2CN
i W \ ~CH2~n ~ cxxr~p wherein n and W are as hereinbefore defined, typically by refluxing in an aprotic solvent, such as chloroform, in the presence of polyphosphate ester.
Compounds of formula (XXXX) may be prepared by reacting a compound of formula (II) wherein n and W are as hereinbefore defined with 3-cyanopropanal, or a carbonyl-protected form thereof, such as (the diethyl acetal, typically in an aqueous acid, for example, dil. HC1.
Compounds of formula (XXXI) may be prepared by reducing a compound of formula (XXXII) H
(XXXII) W
~ (CH2)n SPh wherein n and W are as hereinbefore defined, typically by heating with Raney nickel in a polar solvent, such as IPA.
Compounds of formula (XXXII) may be prepared by reacting a hydrazine of formula (II) wherein n and W are as hereinbefore defined with phenylthioacetaldehyde, or a carbonyl-protected form thereof, for example, the diethyl acetal, in a polar solvent, such as acidified ethanol.

For a better understanding of the invention, the following Examples are given by way of illustration.
SYNTHETIC AND REFERENCE EXAMPLES
Synthetic Example 1 Preparation of (S)-2-f5-(2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indol-3-yllethylamine (a) (S)-Methyl 4-nitrophenvlalanate hydrochloride Methanol (110m1) was treated dropwise with thionyl chloride (26.3g) at -10°C and L-4-nitrophenylalanine (Fluka, 21.7g) added to the resulting solution as a solid. The mixture was stirred overnight at room temperature and the methanol removed in vacuo to give the desired product as a pale yellow solid (21.2g) .
(b) (S)-2-Amino-3-(4-nitrophenyl)propanol The product from step (a) (21.2g) was dissolved in ethanol/water (190m1, 100/90 v/v) and the solution added dropwise at 0°C to a stirred solution of sodium borohydride (l3.Og) in ethanol/water (190m1, 100/90 v/v). The resulting mixture was refluxed for 2.5 hours, cooled and the precipitate filtered off.
The ethanol was partially removed from the filtrate in vacuo and the resulting precipitate filtered off and dried to give the desired product as a pale yellow solid (7.5g).

(c) (S)-4-(4-Nitrobenzyl)-1,3-oxazolidin-2-one The product from step (b) (4.9g) was suspended in toluene, the suspension, cooled to 0°C and a solution of potassium hydroxide (7.Og) in water (56m1) added dropwise. A solution of phosgene (62.5m1 of a 12%
w/v solution in toluene) was added dropwise to the resulting solution over 30 minutes and stirring continued for 1 hour. The mixture was extracted with ethyl acetate and the extracts washed with brine, dried and evaporated in vacuo to give a yellow oil. Crystallisation from ethyl acetate gave the desired product as pale yellow crystals (2 . 3g) .
(d) (S)-4-(4-Aminobenzyl)-1,3-oxazolidin-2-one hydro-chloride A suspension of the product from step (c) (0.79g) and 10% palladium on carbon (0.26g) in a mixture of ethanol (15m1), water (llml), ethyl acetate (2.Om1) and aqu. 2N HC1 (2.3m1) was stirred under 1 atmos.
pressure of hydrogen until uptake ceased. The mixture was filtered through Hyflo (Trade-mark) and the filtrate evaporated in vacuo to give the desired product as a pale yellow foam (0.79g).

(e) (S)-4-(4-Hydrazinobenzyl)-1,3-oxazolidin-2-one hydrochloride The product from step (d) (0.79g) was suspended in water (4.8m1) and c.HCl (8.lml) added dropwise. The resulting mixture was cooled to -5°C and a solution of sodium nitrite (0.24g) in water (2.4m1) added dropwise to the stirred mixture. over 15 minutes followed by 30 minutes stirring at -5 to 0°C. The solution was then added at 0°C over 15 minutes to a stirred solution of tin (II) chloride (3.Sg) in c.HCl (6.9m1), followed by 3 hours stirring at room temperature. The solution was evaporated in vacuo and the residue triturated with ether to give the desired product as a pale yellow solid (0.96g).
(f) (S)-2-f5-(2-Oxo-1 3-oxazolidin-4-ylmethyl)-1H-indol-3_yllethylamine The product from step (e) (0.84g) was dissolved in ethanol/water (125m1, 5:1) and the solution treated with 4-chlorobutanaol dimethylacetal (JACS 1365 (1951), 0.52g). The mixture was refluxed for 2 hours, the solvent removed in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H
(30:8:1) as eluant. The desired product was obtained as a colourless oil (0.21g).

Salt of Synthetic Example 1 Maleate Ethanolic malefic acid (1.0 equiv.) was added dropwise to the free base (0.21g) and the ethanol evaporated in vacuo. The resulting gum was freeze-dried from water to give the desired product as a white lyopholate (0.22g), [a] 21D -5. 92° (c = 0.3, MeOH) .
1H NMR (DMSO-d6, b) : 2.7-3.5 (6H, m, CH2) , 3.35 (2H, s, NHz) , 4.05 (2H, m, CHZ) , 4.25 (1H, m, CH) , 6.05 (2H, s, malefic acid) , 6.98 (1H, d, Ar) , 7.2 (1H, s, Ar) , 7.3 (1H, d, Ar), 7.4 (1H, s, Ar), 7.75 (1H, s, NH) and 10.9 (1H, s , NH ) .
Microanalysis: C 55.03 (54.96), H 5.54 (5.85), N 10.30 (10.68) .
Synthetic Example 2 Preparation of (S)-N N-dimethYl-2-f5-(2-oxo-1,3-oxazoli-din-4-ylmethyl)-1H-indol-3-yllethylamine 0.9 isoprot~anol-ate 0.5 hydrate A solution of formaldehyde (0.03g) in methanol (1.8m1) was added to a solution of the free base from step (f) of Synthetic Example 1 (0.12g) and sodium cyanoborohydride (0.04g) in a mixture of methanol (5.5m1) and glac. acetic acid (0.14g) and the resulting mixture stirred overnight at room temperature. The pH was adjusted to 8.0 using aqu. KZC03 and the mixture extracted with ethyl acetate.
The combined extracts were washed with brine, dried and evaporated to give a colourless oil (0.14g) which crystallised from isopropanol to give the desired product as a white crystalline solid (O.lOg), mp 139-141°C.
1H NMR (DMSO-ds, 8) : 2.2 (6H, s, NMe2~, 2.5 (2H, m, CHZAr) , 2 .7-3 . 0 (4H, m, CHz) , 4.1 (2H, m, CH2) , 4.3 (1H, m, CH) , 6.9 (1H, d, Ar), 7.1 (1H, s, Ar), 7.3 (1H, d, Ar), 7.4 (1H, s, Ar) , 7.7 (1H, s, NHCO) and 10.7 (1H, s, NH) .
Microanalysis: C 64.26 (64.11), H 8.28 (8.34), N 12.02 (12.00) [a] 22D - 5 . 79° (c = 0 . 5, MeOH) Salts of Synthetic Example 2 Maleate A solution of malefic acid (0.17g) in ethanol (5m1) was added to a solution of the free base (0.5g) in ethanol (5m1). The mixture was evaporated in vacuo and the resulting oil triturated with ether and methanol to give the maleate salt as a white solid which was recrystallised from ethanol (0.45g), mp 151-152°C.

Hydrochloride Ethereal HC1 (1.1 equivs.). was added dropwise to a stirred solution of the free base (0.35g) in methanol (lml) at 0°C. The hydrochloride salt precipitated as an oil. The mixture was evaporated in vacuo and the resulting foam crystallised from isQpropanol to give the desired product as a white solid (0.36g), mp 118-120°C, [a] 23D -9 . 35 (c = 0 . 31, water) .
Succinate A solution of succinic acid (0.36g) in ethanol (lOml) was added to a solution of the free base (l.Og) in ethanol (lOml). The mixture was evaporated in vacuo and the resulting foam triturated with isopropanol to give the succinate salt as a white solid (l.Og), mp 122-123°C.
Benzoate A solution of benzoic acid (0.37g) in ethanol (lOml) was added to a solution of the free base (l.Og) in ethanol (lOml). The mixture was evaporated in vacuo and the resulting foam crystallised from ethyl acetate to give the benzoate salt as a white solid (0.74g), mp 90-92°C.

Synthetic Example 3 Alternative preparation of (S)-N N-dimethyl-2-f5-(2-oxo-1 3-oxazolidin~lmethyl)-1H-indol-3-yllethylamine 0.9 iso-propanolate 0.5 hydrate 4-Dimethylaminobutanal diethylacetal (Croatica Chemica Acta 36, 103 (1964), 3.9g) was added to a solution of the product from step (e) of Synthetic Example 1 (10.4g) in a mixture of acetic acid (50m1) and water (150m1) and the resulting mixture refluxed for 4.5 hours. The mixture was cooled, evaporated in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H (50:8:1) as eluant to give the desired product as a pale yellow oil which crystallised from isopropanol as a white crystalline solid (3.5g), mp 138-140°C. 1H NMR, microanalysis and [a]D as for product of Synthetic Example 2.
Reference Example 4 Preparation of (~)-3-(1-methyl-4-piperidyl)-5-(2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indole (a) 3-(1-Methyl-1 2,3,6-tetrahydro-4-pyridyl)-1H-indole-5-carbonitrile 5-Cyanoindol (Aldrich, 20.Og) was added to a solution of KOH (22.4g) in methanol (200m1). N-Methyl-4-piperidone (Aldrich, 40.4g) was then added dropwise and the resulting mixture refluxed for 4 hours, then cooled and poured into water. The resulting precipitate was filtered off and dried to give the desired product as a pale pink crystalline solid (32.6g) .
(b) 3-(1-Methyl-1,2,3,6-tetrahydro-4-pyridyl)-1H-indole-5-carbaldehyde Raney nickel (ca lOg) was added to a solution of the product from step (a) (5.Og) and sodium hypophos-phite (6.Og) in a mixture of water (25m1), glac.
acetic acid (25m1) and pyridine (50m1) at 45°C.
The resultin mixture was stireed at 45°C for 1 hour, cooled and basified to pH 9 with 0.88 NH40H. The mixture was filtered through Hyflo and the filtrate extracted with chloroform. The combined extracts were dried and evaporated in vacuo to give the desired product as an off-white solid which was recrystallised from ethanol (2.4g).
(c) 5-f3-(1-Methyl-1,2,3,6-tetrahydro-4-pyridyl)-1H
indol-5-ylmethylenel-2,4-imidazolidinedione A mixture of the product from step (b) (2.4g), hydantoin (Aldrich, 0.98g) and ammonium acetate (0.74g) in glac. acetic acid (2.4m1) was heated at 120°C for 4 hours. The mixture was cooled and the resulting precipitate filtered off and dried to give the desired product as,a yellow solid (2.4g).

(d) (~) -5- (2 , 5-Dioxo-4-imidazolidinylmethyl) -3- (1-methyl-4-piperidyl)-1H-indole The product from step (c) (2.4g) was suspended in a mixture of water (100m1) and ethanol (200m1) and 10% w/w Pd/C (0.25g) added. The mixture was stirred under 1 atmos. pressure of hydrogen for 17 hours when uptake was complete. The mixture was filtered through Hyflo and the filtrate evaporated in vacuo to give the desired product as a colourless solid (2.4g) .
(e) (~) -3- f3- (1-Methyl-4-piperidyl) -1H-indol-5-yll -alanine A solution of the product from step (d) (2.4g) and barium hydroxide hydrate (8.4g) in water (50m1) was refluxed for 72 hours, then cooled and evaporated in vacuo. The residue was taken up in hot methanol and filtered to remove barium salts. The filtrate was evaporated in vacuo, the residue dissolved in water and dry ice added to precipitate barium carbonate. The latter was filtered off and the filtrate evaporated in vacuo to give the desired product as a yellow foam (1.3g).
(f) (~)-Methyl 3-f3-(1-methyl-4-piperidyl)-1H-indol-5-yllalanate A solution of the product from step (e) (6.2g) in methanol (40m1) was added dropwise to a solution of thionyl chloride (2.9m1) in methanol (35m1) at -10°C. The resulting mixture was stirred overnight at room temperature, then evaporated in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H (30:8:1) as eluant. The eluate was evaporated in vacuo to give the desired product as a yellow foam (4.8g) .
(g) (+) -3- [3- (1-Methyl-4-piperidyl) -1H-indol-5-vll -2-amino-1-propanol A solution of the product from step (f) (4.8g) in water (20m1) and ethanol (20m1) was added dropwise to a suspension of sodium borohydride (0.61g) in a mixture of water ( 2 Oml ) and ethanol ( 2 Oml ) at 0°C .
The resulting mixture was refluxed for 3 hours, then evaporated in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H
(30:8:1) as eluant. The eluate was evaporated in vacuo to give the desired product as a colourless foam ( 1 . 6g) .
(h) (+) -3- (1-Methyl-4-giperid~l) -5- (2-oxo-1, 3-oxazo-lidin-4-ylmethyl)-1H-indole A mixture of the product from step (g) (1.6g), diethyl carbonate (0.73m1) and potassium carbonate (0.08g) was heated at 130°C for 5 hours. The mixture was cooled, taken up in methanol and the insoluble potassium carbonate filtered off. The filtrate evaporated in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H
(30:8:1) as eluant. The eluate was evaporated in vacuo and the residue recrystallised from isopro-panol/ether to give the desired product as a colour-less crystalline solid (l.lg), mp 191-192°C.
1H NMR (DMSO-d6, b) : 1.6-1.8 (2H; 2 x CHNMe) , 2 .8-2 .1 (4H, 2 x CHZ) , 22.2 (3H, s, NMe) , 2.6-3.0 (2H, 2 x CHNMe: 1H, CH: 2H, CHZAr), 3.9-4.1 (2H, m, CH20), 4.2-4.4 (1H, m, CHN), 6.9 (1H, d, Ar), 7.1 (1H, d, Ar), 7.3 (1H, d, Ar), 7.4 (1H, s, Ar), 7.8 (1H, s, NHCO) and 10.7 (1H, S, NH).
Salt of Reference Example 4 Hydrochloride c.HCl (1.0 equiv.) was added dropwise to a stirred solution of the free base (l.lg) in ethanol (5m1) at 5°C.
The addition of ether to the resulting mixture precipitated the desired product as a white solid (l.lg), mp 235-236°C (dec) .

Reference Example 5 Alternative preparation of (+)-3-(1-methyl-4-piperidvl)-5-(1 3-oxazolidin-4-ylmethyl)-1H-indole (a) 1H-Indole-5-carbaldehyde Raney nickel (6.7g) was added to a solution of 5-cyanoindole (Aldrich, lO.Og) and sodium hypophos-phite (20.0 g) in a mixture of water (73m1), glac.
acetic acid (73m1) and pyridine (145m1) at 45°C for 2 hours, then cooled and filtered through Hyflo. The filtrate was diluted with water and extracted with ethyl acetate. The combined extracts were washed with water, 10% aqu. citric acid. 1N aqu. HC1, water and brine, dried and evaporated in vacuo to give the desired product as a buff solid which was recrystallised from chloroform (7.5g).
(b) 5- (2-nitroethen~l) -1H-indole A mixture of the product from step (a) (7.5g), ammonium acetate (1.5g) and nitromethane (77m1) was heated at 110°C for 2 hours, then cooled and evaporated in vacuo. The residue was triturated with water to give the desired product as a yellow solid which was filtered off and dried (9.2g).

(c) 5-(2-Nitroethyl)-1H-indole A solution of sodium borohydride (2.Og) and 40%
w/v aqu. NaOH was added dropwise to a solution of the product from step (b) (1.9g) in acetonitrile (55m1) at 0°C. The pH was maintained at 3-6 by periodic additions of 2N aqu._ HC1. The resulting solution was stirred at 0°C for 2 hours, then diluted with water and extracted with DCM. The combined extracts were washed with brine, dried and evaporated in vacuo to give a yellow oil which was eluted through a silica column using chloroform as eluant to give the desired product as a pale yellow oil (0.78g).
(d) 3~1-Methyl-1, 2 , 3 , 6-tetrahydro-4-wridyl) -5- (2-nitroethyl)-1H-indole N-Methyl-4-piperidone (Aldrich, 4.2g) was added to a solution of the product from step (c) (2.3g) in glac. acetic acid (35m1) at 100°C for 1 hour, cooled and poured into a mixture of 0.88 NH40H
(6lml) and ice (61g). The resulting solid was filtered off, dried and recrystallised from ethanol to give the desired product as a white solid (1.6g) .

(e) (~)-3-f3-(1-Methyl-1,2,3,6-tetrahydro-4-pyridyl)-1H-indol-5-yll-2-amino-1-propanol Sodium methoxide (0.30g) was added to a solution of the product from step (d) (1.5g) in DMF (l5ml) at 0°C. To the resulting solution was added dropwise a suspension of paraformaldehyde (0.19g) 9n DMF
(20m1). The resulting mixture was stirred at 0°C
for 1.5 hours, then poured into water and extracted with ethyl acetate. The combined extracts were washed with water and brine, dried and evaporated in vacuo to give a yellow oil which was eluted through a silica column using DCM/EtOH/NH40H
(50:8:1) as eluant to give the desired product as an off-white solid (0.85g which was recyrstallised from ethanol.
(f) (~)-3-f3-(1-Methyl-4-piperidyl)-1H-indol-5-yll-2-amino-1-pro~anol The product from step (e) (0.08g) was dissolved in ethanol (25m1) and 10% w/w Pd/C (0.23g) added. The mixture was stirred under 1 atmos. pressure of hydrogen for 7 hours when uptake was complete. The mixture was filtered through celite and the filtrate evaporated in vacuo to give the desired product as colourless oil which was eluted through a silica column using DCM/EtOH/NH40H (50:8:1) as eluant.

(g) (~)-3-(1-Methyl-4-piperidyl)-5-(1,3-oxazolidin-4-ylmethyl)-1H-indole A mixture of the product from step (f) (1.6g), diethyl carbonate (0.71g) and potassium carbonate (0.08g) was heated at 130°C for 5 hours. The mixture was cooled, taken up in-methanol and the insoluble potassium carbonate filtered off. The filtrate was evaporated in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H
(30:8:1) as eluant to give a colourless foam which was crystallised from isopropanol/ether to give the desired product as a colourless crystalline solid (l.lg), mp 191-192°C. 1H NMR and microanalysis as for product of Reference Example 4.
Synthetic Example 6 Preparation of (R)-2-[5-(2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indol-3-yllethylamine (a) (R)-4-(4-nitrobenzyl)-1,3-oxazolidin-2-one A solution of D-4-nitrophenylalanine (Fluka, 53g) in dimethoxyethane (250m1) was warmed to 67°C and BF3.Et20 (Aldrich, 37m1) added over 1 hour. The resulting solution was stirred at 67°C for 1 hour, then heated to 80°C and BH3,Me2S (Aldrich, 40m1) added over 1 hour at 80-85°C. The resulting solution was heated at 85°C for 4 hours, then cooled and methanol (40m1) added. The solution was heated to 85°C and the solvents removed by distillation to 1/3 of the original bulk. 6N aqu. NaOH (136m1) was added to the hot solution which was then heated at 85°C for hour, cooled and DCM (100m1) added. The solution was cooled to -15 to -20°C and a solution of tri-chloromethyl chloroformate (Aldrich, 18.2m1) in DCM
(23m1) added at below -10°C. The pH was maintained at 9-11 by periodic additions of 6N aqu. NaOH. The resulting solution was stirred at room temperature for 1 hour, then diluted with water and extracted with DCM. The combined extracts were washed with water and brine, dried and evaporated in vacuo to give the desired product as a pale brown solid which was recrystallised from ethyl acetate to give a pale yellow solid (35g) , mp 113-115°, [a] 21D +46 .47°
(c = 0.56, MeOH).
(b) (R)-4-(4-Aminobenzyl)-1,3-oxazolidin-2-one hydro-chloride The product from step (a) (lO.Og) was suspended in a mixture of water (120m1), ethanol (60m1) and 2N aqu.
HC1 (22.5m1) and 10% w/w Pd/C (l.Og) added. The mixture was stirred under 1 atmos. pressure of hydrogen for 8 hours when uptake was complete. The mixture was filtered through Hyflo (Trade-mark for a finely divided filtration material) and the filtrate evaporated in vacuo to give the desired product as a colourless glass (10.3g).

(c) (R)-4-(4-Hydrazinobenzyl)-1,3-oxazolidin-2-one hvdrochloride The product from step (b) (10.3g) was suspended in water (53m1) and c.HCl (106m1) added dropwise. The resulting mixture was cooled to -5°C and a solution of sodium nitrite (3.2g) in water (30m1) added drop-wise to the stirred mixture over 15 minutes followed by 30 minutes stirring at -5 to-OC°. The solution was then added at 0°C over 15 minutes to a stirred solution of tin (II) chloride (51g) in c.HCl (9lml), followed by 3 hours stirring at room temperature.
The solution was evaporated in vacuo and the residue triturated with ether to give the desired product as a pale yellow solid (llg).
(d) (R)-2-(5-(2-Oxa-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl)-ethylamine The product from step (c) (8.8g) was dissolved in ethanol/water (500m1, 5:1 v/v) and the solution treated with 4-chlorobutanal dimethylacetal (J.
Amer. Chem. Soc. 1365 (1951), 5.5g). The mixture was refluxed for 2 hours, the solvent removed in vacuo and the residue eluted through a silica column using DCM/EtOH/NH40H (30:8:1 v/v/v) as eluant. The desired product was obtained as a pale yellow oil (0.6og) .

Salt of Synthetic Example 6 Hydrochloride c.HCl (0.06m1) was added dropwise to a stirred solution of the free base (0.16g) in ethanol (2m1) at 0°C. The hydrochloride salt was precipitated.as a fawn solid, mp 269-271°C, [a] Z1D t5. 88° (c = 0 .27, MeOH) .
Synthetic Example 7 Preparation of (R)-N N-dimethyl-2-f5-(2-oxo-1,3-oxazoli-din-4-ylmethyl)-1H-indol-3-yllethylamine A solution of 35% w/v aqu. formaldehyde (0.3m1) in methanol (2.Om1) was added to a solution of the product from step (d) of Synthetic Example 6 (0.44g) and sodium cyanoborohydride (0.13g) in a mixture of methanol (8.5m1) and glac. acetic acid (0.51g) at 10°C and the resulting mixture stirred at room temperature for 2.5 hours. 2N
aqu. NaOH (l.3ml) was added, then sodium borohydride (0.19 g) followed by 2N aqu. HCl (1.3 ml) . The methanol was evaporated in vacuo and the remaining solution diluted with water, taken to pH 7 with solid potassium carbonate and washed with ethyl acetate. Further potassium carbonate was added to Ph 11 and the solution extracted with ethyl acetate. The combined extracts were evaporated in vacuo to give the desired product as a white foam (0.45g) .

Salt of Synthetic Example 7 Hydrochloride c.HCl (0.16m1) was added dropwise to a stirred solution of the free base (0.45g) in ethanol (4.5m1 at 0°C. The mixture was evaporated in vacuo and the resulting foam triturated with ethyl acetate to give the desired product as a white solid, mp 130°C, [a] Z1D +5 . 15° (c = 0 . 77, MeOH) .
Reference Example 8 Preparation of (S)-N,N-dimethyl-2-f5-(2-thia-1,3-oxazo-lidin-4-~lmethyl)-1H-indol-3-~rllethylamine hydrochloride (a) (S)-N N-Dimethyl-2-[5-(2-amino-1-propanol)-1H-indol-3-yllethy-lamine A solution of the hydrochloride salt of the product of Synthetic Example 2 (0.33g) in 2N aqu. KOH (lOml) was refluxed for 4 hours, then cooled and extracted with ethyl acetate. The combined extracts were dried and evaporated in vacuo to give the desired product as a colourless oil (0.25g).
(b) ~S)-N N-Dimethyl-2-f5-(2-thia-1,3-oxazolidin-4-yl methyl)-1H-indol-3-yllethylamine hydrochloride A solution of N,N'-thiocarbonylimidazole (Aldrich, 0.21g) in THF (4m1) was added dropwise to a stirred solution of the product from step (a) (0.31g) in THF (4ml) and the mixture refluxed for 23 hours, then cooled and evaporated in vacuo. The residue was chromatographed through a silica column using DCM/EtOH/NH40H (20:8:1) as eluant to give the desired product as a colourless oil.
Salt of Reference Example 8 Hvdrochloride 1M Ethanolic HC1 (1.0 equiv.) was added dropwise to the free base and the ethanol evaporated in vacuo. The resulting gum was freeze-dried from water to give the desired product as a white solid (0.17g), mp 133-136°C, (softens 183°C) , [a] 24.sD -29.8 (c = 0 . 5 , water) .
Reference Example 9 Preparation of (S)-2-f5-(3-methyl-2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yllethylamine hydrobromide (a) ~S)-3-Methyl-4-(4-nitrobenzyl)-2-oxazolidinone Sodium hydride (0.808 as a 60% w/w dispersion in oil) was added at room temperature to a stirred solution of the product from step (c) of Synthetic Example 1 (4.4g) in dry THF (150m1). The mixture was stirred for 1.5 hours, then dimethyl sulphate (2.1m1) was added and stirring continued for a further 16 hours. More sodium hydride (0.40g) was added and stirring continued for another 2 hours.

The mixture was evaporated in vacuo and the residue suspended in ethyl acetate and filtered. The filtrate was evaporated in vacuo and the residue crystallised from ethyl acetate/hexane to give the ' desired product as yellow crystals (3.7g), mp 146-147°C, [a] 23D +64 .5° (c = 1 . 0, MeOH) .
(b) (S)-3-Methyl-4-(4-aminobenzyl)-2.-oxazolidinone hydrochloride A suspension of the product from step (a) (4.Og) and 10% w/w Pd/C (0.20g) in a mixture of ethanol (70m1) and dil. HCl (2N aqu. HC1 (12m1) + water (55m1)) was hydrogenated at 45 psi for 1 hour.
The mixture was filtered through Hyflo and the filtrate evaporated in vacuo to give the desired product as a foam.
(c) (S)-3-Methyl-4-(4-hydrazinobenzyl)-2-oxazolidinone hydrochloride A solution of the product from step (b) (4.1g) in water (24m1) was cooled to -5°C and c.HCl (40m1) added. A solution of sodium nitrate (1.2g) in water (12m1) was then added and stirring continued for 0.5 hour. The resulting solution was added dropwise at -5°C to a stirred solution of stannous chloride dehydrate (18.8g) in c.HCl (34m1). The resulting mixture was stirred at 0°C for 2.5 hours, then evaporated in vacuo. The residue was taken up in water, brought to pH 2.5 using lON aqu. NaOH and filtered. The filtrate was evaporated in vacuo and the residue triturated with ethanol and filtered.
The filtrate was evaporated in vacuo to give the desired product as a froth.
(d) (S)-2-[5-(3-Methyl-2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indol-3-yl]ethylamine hydrobromide 4-Chlorobutanal dimethylacetal (J. Amer. Chem. Soc.
1365 (1951) 2.3g) was added to a stirred solution of the product from step (c) (4.4g) in ethanol/-- water (150m1/20m1) and the mixture refluxed for 2 hours. The cooled mixture was evaporated in vacuo, and the residue eluted through a silica column using DCM/MeOH/NH40H (60:8:1) as eluant to give a brown oil (1.7g). A portion of this (0.25g) was taken up in ethanol and treated with an excess of HBr in acetic acid (ca 45% w/v). The resulting solution was evaporated in vacuo and the residue triturated with ether, then crystallised from ethanol/hexane to give the desired product as pale yellow crystals (0.14g), mp 203-205°C, [a] z5D +29. 9° (c = 0. 5, MeOH) .
Elemental analysis and 1H NMR were consistent with the proposed structure.

Reference Example 10 Preparation of (S)-N,N-dimethyl-2-[5-(3-methyl-2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yllethylamine maleate 0.75 hydrate Sodium cyanoborohydride (0.14g) followed by glac. acetic acid (0.54m1) were added at room temperature to a stirred solution of the free base (0.52g) from step (d) of Reference Example 9 in methanol (9.Om1). When effervescence was complete, a solution of 37% w/v aqu.
formaldehyde (0.16g) in methanol (2.Om1) was added and the mixture stirred for 1 hour, then diluted with water, saturated with potassium carbonate and extracted with ethyl acetate. The combined extracts were evaporated in vacuo and the residue eluted through a silica column using DCM/MeOH/NH40H (60:8:1) as eluant to give the free base of the desired product as a colourless oil (0.25g).
The latter was dissolved in ethanol (lOml), treated with a solution of malefic acid (0.09g) in ethanol (lml) and the resulting solution evaporated in vacuo to give an oil which was triturated with ether, the freeze-dried from water to give the desired product as a colourless glass, [a] ZZD +24 . 5° (c - 0 . 5, MeOH) . Elemental analysis, 1H NMR
and MS were consistent with the proposed structure.

Synthetic Example 11 Preparation of (S)-N-benzyl-2-(5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl)ethylamine maleate 0.75 hydrate Benzaldehyde (0.70g) was added at room temperature to a stirred solution of the compound of. Synthetic Example 1 (1.7g) in ethanol (20m1). The solution was stirred for 36 hours, then sodium borohydride (0.25g) was added in portions and stirring continued for a further 2 hours.
The solution was evaporated in vacuo and the residue cooled, acidified with 2N aqu. HC1, basified with sodium bicarbonate, saturated with potassium carbonate and extracted with ethyl acetate. The combined extracts were evaporated in vacuo to give an oil which was eluted through a silica column using DCM/EtOH/NH40H (100:8:1) as eluant to give the free base of the desired product as a yellow froth (1.6g). A portion of this (0.13g) was dissolved in ethanol (lOml), treated with a solution of malefic acid (43mg) in ethanol (lml) and the resulting solution evaporated in vacuo. The residue was freeze-dried from water to give the desired product as a pale yellow powder (0.16g) [a]24D +1.4° (c - 0.5, MeOH).
Elemental analysis, 1H NMR and MS were consistent with the proposed structure.

Synthetic Example 12 Preparation of (S)-N-benzyl-N-methyl-2-(5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl)ethylamine maleate hydrate Anhy. potassium carbonate (0.34g), was added at room temperature to a solution of the free base of Synthetic Example 11 (0.45g) in DMF (8.Om1). The suspension was stirred for 0.5 hour, then a solution of dimethyl sulphate (0.17g) in DMF (2.Om1) was added and stirring continued for a further 3 hours. Water (40m1) was added and the mixture extracted with ethyl acetate. The combined extracts were evaporated in vacuo to give a yellow oil which was eluted through a silica column using DCM/EtOH/NH40H (100:8:1) as eluant to give the free base of the desired product as a colourless oil (0.32g). A
portion of this (73mg) was dissolved in ethanol (lOml), treated with a solution of malefic acid (23mg) in ethanol (lml) and the resulting solution evaporated in vacuo.
The residue was freeze-dried from water to give the desired product as a pale yellow powder, [a]24D +3.1° (c -0.5, MeOH). Elemental analysis, 1H NMR and MS were consistent with the proposed structure.

Synthetic Example 13 Preparation of (S)-N-methyl-2-[5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yllethylamine maleate 0.5 hydrate A suspension of the free base of the product of Synthetic Example 12 (0.25g) and 10% w/w Pd/C (O.lOg) in ethanol (25m1) was hydrogenated for 16 hours. The mixture was filtered through Hyflo and the filtrate evaporated in vacuo. The residue was eluted through a silica column using DCM/EtOH/NH40H (30:8:1) as eluant to give the free base of the desired product (0.14g). The latter was dissolved in ethanol (lOml), treated with a solution of malefic acid (0.06g) in ethanol (lml) and the resulting solution evaporated in vacuo. The residue was freeze-dried from water to give the desired product as a hygroscopic solid, [a] 25D -5 .4° (c - 0 . 5, MeOH) .
Elemental analysis and 1H NMR were consistent with proposed structure.

Reference Example 14 Preparation of (S)-3-(1-methyl-1,2,3,6-tetrahydro-4-gyridyl)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indole 0.33 methanolate 0.75 hydrate (a) (S)-3-Phenylthio-5-(2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indole Phenylthioacetaldehyde diethylacetal (JCS. Chem.
Comm. 924 (1978), 9.1g) was added at room temperature to a stirred solution of the product from step (e) of Synthetic Example 1 (9.8g) in a mixture of ethanol (150m1) and water (100m1).
c.HCl (5 drops) was added and the mixture stirred at room temperature for 2 days, then partially evaporated in vacuo. The resulting aqueous sus-pension was extracted with ethyl acetate,and the combined extracts washed with water and evaporated in vacuo to give a brown oil. The latter was eluted through a silica column using DCM/EtOH/NH40H
(150:8:1) as eluant to give the desired product as a pale yellow oil (5.Og).
(b) (S)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indole Raney nickel (3.Og) was added to a solution of the product from step (a) (3.1g) in IPA (150m1) and the suspension refluxed for 1 hour. More Raney nickel (2.Og) was added and refluxing continued for a further 2 hours. The suspension was filtered hot through Hyflo and the filtrate evaporated in vacuo to give an oil. the latter was eluted through a silica column using ethyl acetate as eluant to give the desired product as a froth (1.3g). 1H NMR and MS
were consistent with the proposed structure.
(c) (S)-3-(1-Methyl-1,2,3,6-tetrahydro-4-pyridyl)-5-(2-oxo-1 3-oxazolidin-4-ylmethyl)-1H-indole 0.33 methanolate 0.75 hydrate 1-Methyl-4-piperidone (0.478, Aldrich) was added to a stirred solution of the product from step (b) (0.30g) in glac. acetic acid (2.Om1) and the mixture stirred at 100°C for 2 hours. The cooled mixture was poured onto ice/NH40H (20m1) and the resulting solid filtered off. The latter was eluted through a silica column using DCM/EtOH/NH40H
(60:8:1) as eluant and crystallised from ethyl acetate to give the desired product as a colour-less solid (O.llg) , mp 225-227°C, [a,] 2°D -45.4° (c =
0.5. 1N aqu. HC1). Elemental analysis and 1H NMR
were consistent with the proposed structure.

Reference Example 15 Preparation of (S)-3-(1-methyl-4 ~iperidyl)-5-oxo-1,3-oxazolidin-4-ylmeth~rl)-1H-indole hydrobromide A suspension of the product of Reference Example 14 (0.35g) and 10% w/w Pd/C (O.lOg) in a mixture of methanol (lOml), water (lOml) and 1N aqu. Hcl was hydrogenated for 5 hours. The mixture was filtered through Hyflo and the filtrate evaporated in vacuo. The residue was basified with NH40H, evaporated in vacuo and eluted through a silica column using DCM/EtOH/NH40H (45:8:1) as eluant to give an oil. The latter was taken up in ethanol (5.Oml) and treated with an excess of HBr in acetic acid (ca 45%
w/v) to give the desired product as colourless crystals (0 .20g) , mp 260-261°C [a] Z1D -5.2° (c - 0.5, water) .
Elemental analysis and 1H NMR were consistent with the proposed structure.
Reference Example 16 Preparation of (R)-3-(1-methyl-1,2,3,6-tetrahydro-4-pyridyl)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol hydrate (a) (R)-4-(4-Hydrazinobenzyl)-1,3-oxazolidin-2-one hydrochloride By steps identical to steps (a) to (c) of Synthetic Example 6, D-4-nitrophenylalanine was converted to -Sg-(R)-4-(4-hydrazinobenzyl)-2-oxazolidinone hydro-chloride.
(b) (R)-3-(1-Methyl-1,2,3,6-tetrahydro-4-pyridyl)-5-(2-oxo-1,3-oxazolidin-4-yl)-1H-indole hydrate By steps analogous to steps (a) to (c) of Reference Example 14, the product from step (a) was converted to (R)-3-(1-methyl-1,2,3,6-tetrahydro-4-pyridyl)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indole hydrate, mp 229-231°C, [a]1aD +24.9° (c = 0.5. 1N aqu. HCl).
Elemental analysis and 1H NMR were consistent with the proposed structure.
Reference Example 17 Preparation of (R)-3-(1-methyl-4-piperidyl)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indole hydrobromide.
By a method analogous to that of Reference Example 15, the product of Synthetic Example 16 was converted to (R)-3-(1-methyl-4-piperidyl)-5-(2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indole hydrobromide, mp 260-261°C, [a]19D +4.6°
(c - 0.5, water). Elemental analysis and 1H NMR were consistent with the proposed structure.

Reference Example 18 Preparation of (R)-3-(1-benzyl-1 2 3 6-tetrahydro-4-wridyl)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indole hydrate 1-Benzyl-4-piperidone (Aldrich, 2.8g) was added to a stirred suspension of (R)-5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indole (l.Og), the immediate precursor of the product of Reference Example 16, in glac. acetic acid (20m1) and stirred at 100°C for 3 hours. The cooled mixture was evaporated in vacuo and the residue taken up in methanol, basified with NH40H and evaporated in vacuo to give a dark tar. The latter was eluted through a silica column using DCM/EtOH/NH40H (100:8:1) as eluant and treated with DCM. The resulting precipitate was filtered off to give the desired product as yellow crystals (0.25g), mp 169-170.5°C. Elemental analysis and 1H NMR
were consistent with the proposed structure.
Reference Example 19 Preparation of (R) -3- (4-piperidyl) -5- (2-oxo-1 3-oxazo-lidin-4-ylmethyl)-1H-indol hydrobromide A suspension of the product from Reference Example 18 (0.25g) and 10% w/w Pd/C (O.TOg) in methanol (25m1) was hydrogenated at 90 psi for 20 hours when uptake ceased.
The mixture was filtered through Hyflo and the filtrate evaporated in vacuo. The residue was eluted through a silica column using DCM/EtOH/NH40H (30:8:1) as eluant to give an oil. The latter was taken up in IPA and treated with an excess of HBr in acetic acid (ca 45% w/v) to give a hygroscopic solid which was freeze-dried from water to give the desired product as a pale brown powder.
Elemental analysis and 1H NMR were consistent. with the proposed structure.
Reference Example 20 Preparation (~)-N,N-dimethyl-2-f5-(1-thio-2-thia-3-oxazo-lidin-4-ylmethyl)-1H-indol-3-yl]ethylamine acetate Carbon disulphide (90,1) was added to a stirred solution of the product from step (a) of Reference Example 8 (0.31g) and potassium hydroxide (0.08g) in ethanol (3.8m1) and the mixture refluxed, then evaporated in vacuo. The residue was extracted with ether, acidified and chromatographed using a silica reverse phase HPLC
column and eluting with 10-a90% v/v water/acetonitrile with O.1M aqu. ammonium acetate buffer at pH 4.0 over 20 minutes to give the desired product (O.Olg) and, after treatment with HCl, the product of Reference Example 8 (O.llg) . Both were freeze-dried from water and gave 1H
NMR and MS which were consistent with the proposed structures.

Synthetic Example 21 Preparation of (~)-N,N-dimethyl-2-f5-(2-oxo-2 3-oxazo-lidin-5-vlmethyl)-1H-indol-3-yllethylamine hydrochloride (a) (~)-1-Nitromethyl-2=phenylethanol Sodium methoxide (l.lg) was added to a stirred solution of nitromethane (Aldrich, 12.2g) in methanol (100m1) at 0°C and the mixture stirred for minutes. A solution of phenylacetaldehyde (Aldrich, 24.Og) in methanol (50m1) was added drop-wise over 15 minutes and the mixture stirred for 45 minutes at 0°C, then brought to room temperature over 1 hour and stirred overnight. The mixture was evaporated in vacuo and the residue taken up in water and extracted with ether. The combined extracts were washed with water and brine and evaporated in vacuo to give the desired product as a yellow oil (29.Og).
(b) (~)-1-Aminomethyl-2-phenvlethanol hydrochloride A suspension of the product from step (a) (lO.Og) and 10% w/w Pd/C (l.Og) in ethanol (250m1) was hydrogenated until uptake ceased. The mixture was filtered through Hyflo and the filtrate evaporated in vacuo. The residue was taken up in ethyl acetate and extracted with 2N aqu. HC1. The combined extracts were washed with ethyl acetate, then evaporated in vacuo to give the desired product as a pinkish white solid (6.8g).

(c) (~)-5-Benzyl-1,3-oxazolidin-2-one A solution of KOH (9.4g) in water (85m1) was added to a stirred solution of the product from step (b) (5.1g) in toluene (150m1) at 0°C. A solution of phosgene (9.8g) in toluene (78.4m1 - 12.5% w/v) was added dropwise over 15 minutes and the mixture brought to room temperature, then stirred overnight.
The aqueous phase was separated and extracted with ethyl acetate. The combined extracts were evaporated in vacuo to give the desired product as a white solid (2.2g), mp 106-108°C. Elemental analysis was consistent with the proposed structure.
(d) (~) -5- (4-Nitrobenzyl) -1, 3-oxazolidin-2-one c.H2S04 (1.6m1) was added to the product from step (c) at 0°C followed by c.HN03 (0.33m1, ca 0.05m1/5 minutes) also at 0°C. The mixture was stirred for 0.5 hour at 0°C and then for 0.5 hour at room temperature. Water/ice (100m1) was added and the mixture extracted with ethyl acetate. The combined extracts were evaporated in vacuo to give a yellow oil which was recrystallised from ethyl acetate to give the desired product as a white powder (0.4g), mp 143-146°C.
(e) (~)-5-(4-Aminobenzyl)-1,3-oxazolidin-2-one hydro-chloride A suspension of the product from step (d) (1.4g) and 10% w/w Pd/C (0.14g) in a mixture of water (21m1), ethanol (28m1) and 2N aqu. HCl (3.2m1) was hydro-genated for 2 hours when uptake ceased. The mixture was filtered through Hyflo and the filtrate evaporated in vacuo to give the desired product as a pale yellow foam (1.4g).
(f) (~)-N,N-Dimethvl-2-f5-..(.2-oxo-1,3-oxazolidin-5-ylmethyl)-1H-indol-3-yllethylamine hydrochloride c.HCl (14.5m1) was added to a stirred solution of the product from step (e) (1.4g) in water (8.5m1) at 0°C. A solution of sodium nitrite (0.43g) in water (4.3m1) was added dropwise over 15 minutes at 0°C and the mixture stirred for 0.5 hour at 0°C.
The mixture was then added dropwise to a stirred solution of tin (II) chloride (6.8g) in c.HCl (12.4m1) at 0°C over 15 minutes. The mixture was brought to room temperature over 1 hour, then evaporated in vacuo. The residue was taken up in water (30m1), brought to pH 2.5 using lON aqu. NaOH
and the precipitated salts filtered off. 4-Di-methylaminobutanal diethylacetal (Croatica Chemica Acta 36, 103 (1964), l.lg) followed by 'Amberlyst 15' ion exchange resin (Aldrich, 3.Og) was added to the filtrate and the mixture heated for 3 hours at 100°C, filtered and the filtrate evaporated in vacuo.
The residue was treated with hot ethanol, filtered and the filtrate evaporated in vacuo. The residue was triturated with ethyl acetate, filtered and the filtrate evaporated in vacuo. The residue was recrystallised from ethanol to give the desired product as a pale yellow solid (0.75g), mp 280-281°C.

1H NMR and MS were consistent with the proposed structure.
Synthetic Example 22 Preparation of (S)-N,N-dimethyl-2-f5-(2-oxo-1 3-oxazoli-din-4-ylmethyl)-1H-indol-3-yllethylamine (a) (S)-5-(4-Nitrobenzyl-1,3-imidazolidin-2 4-dione Benzyl isocyante (Aldrich, 3.2g) was added to a solution of L-4-nitrophenylalanine (Aldrich, 4.2g) and potassium hydroxide (1.3g) in water (40m1) at 0°C. The mixture was heated at 60-70°C for 2 hours, filtered and the filtrate acidified with c.HCl to give an off-white solid which was filtered off, suspended in 2N aqu. HCl (20m1) and refluxed for 2 hours. The cooled mixture was diluted with water and filtered to give the desired product as a white solid (5.6g) .
(b) (S)-N,N-Dimethyl-2-f5-(2-oxo-1 3-oxazolidin-4-yl-methyl)-1H-indol-3-yllethylamine By steps identical to steps (d) to (f) of Synthetic Example 1 and Synthetic Example 2 or steps (d) and (e) of Synthetic Example 1 and Synthetic Example 3 and steps (e) to (h) of Reference Example 4, the product from step (a) was converted to (S)-N,N-di-methyl-2-[5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl]ethylamine.

Synthetic Example 23 Preparation of (S)-N,N-dimethyl-2-f5-(2-oxo-1 3-oxazo-lidin-4-ylmethyl)-1H-indol-3-yllethylamine (a) (S)-4-(4-Hydrazinobenzyl)-1,3-oxazolidin-2-one hydrochloride By steps analogous to steps (a) to (c) of Synthetic Example 6, L-4-nitrophenylalanine was converted to (S)-4-(4-hydrazinobenzyl)-1,3-oxazolidin-2-one hydrochloride.
(b) (S) -4- (4- f2- (3-cvanopropvlidene) hvdrazinol benzvl 1,3-oxazolidin-2-one 1M aqu. HCl (4.Om1) was added to a solution of the product from step (a) (2.4g) in water (35m1) . 3-Cyanopropanol diethylacetal (Aldrich, 1.7g) was added at room temperature and the mixture stirred for 2 hours. Further acetal (0.20g) was added and the mixture stirred for another 20 minutes. The aqueous phase was decanted from the resulting gum and extracted with ethyl acetate. The extracts were combined with the gum and evaporated in vacuo to give the desired product (2.5g).
(c) (S)-3-Cyanomethyl-5-(2-oxo-1,3-oxazolidin-4-yl-methyl)-1H-indol A solution of the product from step (b) (2.5g) and polyphosphate ester (20.Og) in chloroform (40m1) was refluxed for 20 minutes. Ice was added to the cooled mixture and the chloroform evaporated in vacuo. The remaining aqueous phase was extracted with ethyl acetate and the combined extracts evaporated in vacuo to give the desired product as a pale yellow oil (1.8g).
(d) (S)-N,N-Dimethvl-2-f5-(2-oxo-1 3-oxazolidin-4-yl-methyl)-1H-indol-3-yllethylamine A suspension of the product from step (c) (1.3g) and 10% w/w Pd/C (l.Og) in 30% w/w ethanolic dimethyl-amine (25m1) was hydrogenated for 24 hours and filtered through Hyflo. Fresh Pd/C (0.7g) and ethanolic dimethylamine (5ml) were added to the filtrate and hydrogenation continued for a further 16 hours. The mixture was filtered through a silica column using DCM/EtOH/NH40H (40:8:1) as eluant to give the desired product as a colourless foam (0.3g). Elemental analysis and 1H NMR were con-sistent with the proposed structure.
Reference Examples 24 to 31 By methods analogous to those described in Synthetic and Reference Examples 1 to 23, the following compounds were prepared. The NMR and microanalysis for each compound were consistent with the proposed structure.
24) 2-[5-(3-Methyl-2-oxoimidazolidin=4-ylmethyl)-1H-indol-3-yl]-ethylamine maleate 0.75 hydrate, mp 94-98°C;

25) 2-[5-(3-Methyl-2-oxoimidazolidin-4-ylmethyl)-1H-indol-3-yl]-N,N-dimethylethylamine maleate 0.95 hydrate (white lyopholate);
26) 2-(5-[2-(2,5-Dioxoimidazolidinyl)ethyl]-1H-indol-3-yl)ethylamine hydrochloride hydrate, mp 83-85°C;
27) 2-(5-[2-(2,5-Diozoimidazolidinyl)ethyl]-1H-indol-3-yl)-N,N-dimethylethylamine maleate hydrate (pale yellow lyopholate);
28) 5-[2-(2,5-Dioxoimidazolidinyl)ethyl]-3-(1-methyl-4-piperidinyl)-1H-indole hydrochloride, mp 320-322°C
(dec) ;
29) 2-[5-(5-Methyl-2-oxoimidazolidin-4-ylethyl)-1H-indol-3-yl]ethylamine maleate hydrate, mp 99°C
(softens 88°C) ;
30) 5-[3-(4-Piperidyl)-1H-indol-5-ylmethyl]-2,4-imidazolidinedione acetate 1.4 hydrate, mp 92-93°C;
(sofens 86°C) and 31) 2-[5-(1-Methyl-2-oxo-4-imidazolidinylmethyl)-1H-indol-3-yl]ethylamine diacetate 2.75 hydrate (pale yellow lyopholate).

PHARMACEUTICAL FORMULATION EXAMPLES
In the following Examples, the "active ingredient" may be any compound of formula (I) and/or a physiologically acceptable salt, solvate or physiologically functional derivative thereof.
(1) Tablet formulations ( i ) Oral Mg/tablet A B C

Active ingredient 25 25 25 Avicel (Trade-mark) 13 - 7 Lactose 78 47 -Starch (maize) - 9 -Starch (pregelatinised, NF15) - - 32 Sodium starch glycollate 5 - -Povidone 3 3 -Magnesium stearate 1 1 1 (ii) Sublingual Ma/tablet D E

Active ingredient 25 25 Avicel 10 -Lactose - 36 Mannitol 51 57 Sucrose 3 Acacia - 3 Povidone 3 -Magnesium stearate 1 1 Formulations A to E may be prepared by wet granulation of the first six ingredients with the povidone, followed by addition of the magnesium stearate and compression.
(iii) Buccal Ma/tablet Active ingredient 25 Hydroxypropylmethyl cellulose (HPMC) 25 Polycarbophil 39 Magnesium stearate 1 The formulation may be prepared by direct compression of the admixed ingredients.

(2) Capsule formulations ( i ) Powder Ma/capsule F G
Active ingredient 25 25 Avicel (Trade-mark for a micro-crystalline cellulose) 45 -Lactose 153 -Starch (1500 NF) - 117 Sodium starch glycollate -Magnesium stearate 2 2 Formulations F and G may be prepared by admixing the ingredients and filling two-part hard gelatin capsules with the resulting mixture.
(ii) Liquid fill Mg/capsule H I
Active ingredient 25 25 Macrogol 4000 BP (Trade designation for a polyethylene glycol) 200 -Lecithin - 100 Arachis oil - 100 Formulation H may be prepared by melting the Macrogol 4000 BP, dispersing the active ingredient in the melt and filling two-part hard gelatin capsules therewith.
Formulation I may be prepared by dispersing the active ingredient in the lecithin and arachis oil and filling soft, elastic gelatin capsules with the dispersion.
(iii) Controlled release M~psule Active ingredient 25 Avicel (Trade-mark for a micro-crystalline cellulose) 123 Lactose 62 Triethylcitrate 3 Ethyl cellulose 12 The formulation may be prepared by mixing and extruding the first four ingredients and spheronising and drying the extrudate. The dried pellets are coated with ethyl cellulose as a release controlling membrane and filled into two-part, hard gelatin capsules.

(3) Intravenous injection formulation by weight Active ingredient 2%
Hydrochloric acid ) q.s. to pH 7 Citrate buffer ) Water for Injections to 100%
The active ingredient is taken up on the citrate buffer and sufficient hydrochloric acid added to affect solution and adjust the pH to -. The resulting solution is made up to volume and filtered through a micropore filter into sterile glass vials which are sealed and oversealed.
(4) Intranasal formulation by weight Active ingredient 0.5%
Hydrochloric acid ) q.s. to pH 7 Citrate buffer ) Methyl hydroxybenzoate 0.2%
Propyl hydroxybenzoate 0.02%
Water for Injections to 100%
The active ingredient is taken up in a mixture of the hydroxybenzoates and citrate buffer and sufficient hydrochloric acid added to affect solution and adjust the pH to 7. The resulting solution is made up to volume and filtered through a micropore filter into sterile glass vials which are sealed and oversealed.

(5) Intramuscular injection formulation Active ingredient 0.05 g Benzyl alcohol 0.10 g Glycofurol 75 (Trade-mark) 1.45 g Water for Injections q.s. to 3.00 ml The active ingredient is dissolved in the glycofurol.
The benzyl alcohol is added and dissolved and water added to 3 ml. The mixture is filtered through a micropore filter into sterile glass vials which are sealed and oversealed.

(6) Syrup formulation Active ingredient 0.05 g Sorbitol solution 1.50 g Glycerol 1.00 g Sodium benzoate 0.005 g Flavour 0.0125 ml The sodium benzoate is dissolved in a portion of the purified water and the sorbitol solution added. The active ingredient is added and dissolved. The resulting solution is mixed with the glycerol and made up to the required volume with purified water.

(7) Suppository formulation Ma/suppositorv Active ingredient (63~,m) * 50 Hard Fat, BP (Witepsol H15-(Trade-mark of Dynamit Nobel 1950 * The active ingredient is used as a powder wherein at least 90% of the particles are of 63~m diameter or less.
One-fifth of the Witepsol H15 is melted in a steam-jacketed pan at 45°C maximum. The active ingredient is sifted through a 200~,m sieve and mixed with the molten base using a Silverson mixer fitted with a cutting head until a smooth dispersion is achieved. Maintaining the mixture at 45°C, the remaining Witepsol H15 is added to the suspension which is stirred to ensure a homogenous mix. The entire suspension is then passed through a 250~m stainless steel screen and, with continuous stirring, allowed to cool to 40°C. At a temperature of 38-40°C, 2.Og aliquots of the mixture are filled into suitable plastic moulds and the suppositories allowed to cool to room temperature.

(8) Pessary formulation Mg/pessary Active ingredient (63~m) 50 Anhydrous dextrose 470 Potato starch 473 Magnesium stearate 473 . 1000 The above ingredients are mixed directly and pessaries prepared by compression of the resulting mixture.
BIOLOGICAL ASSAY
The compounds of formula (I) prepared in the Synthetic Examples were each tested for their activity as agonists for the "5-HT1-like" receptor mediating smooth muscle contraction by the following method.
Right and left lateral saphenous veins were obtained from male New Zealand White rabbits (2.4-2.7 kg) which had been killed by intravenous injection of pentobarbitone sodium (60 mg/kg). Ring segments (3-5 mm wide) prepared from each vessel were suspended between two wire hooks and immersed in 20 ml organ baths containing Krebs' solution (pH 7.4) of the following composition (mM): NaCl 118.41, NaHC03 25.00, KCl 4.75, KHzP04 1.19, MgS04 1.19, glucose 11.10 and CaClz 2.50. Cocaine (30~.M) was present in the Krebs' solution throughout the experiment to prevent the uptake of amines by sympathetic neurones.
The Krebs' solution was maintained at 37°C and continually gassed with 95% oxygen/5% carbon dioxide. Increases in tissue isometric force were measured using Grass FT03C
force displacement transducers and recorded on a Gould BD-212 pen recorder.
A force of l.Og was applied to each preparation and re-established twice during a subsequent period of 30 minutes. During this period, tissues were exposed to pargyline (500~M) to irreversibly inhibit monamine oxidase and to phenoxybenzamine (0.1~M) to inactivate a,l-adrenoceptors. At the end of the 30 minutes, the inhibitors were removed by several changes of the organ bath Krebs' solution.
Agonist activity was assessed by cumulative additions of the test compound, its concentration being increased in 0.5 loglo unit increments until further additions caused no further change in tissue force. In each experiment, the activity of the test compound was compared to the activity of 5-HT. Activity was expressed in terms of the p [Aso] (-loglo [M] , where M is the molar concentration of agonist required to produce half the maximum effect).
The results obtained for the compounds of Synthetic Examples 2/3 and References 4/5 are shown in Table 1.
Table 1 Example Activity ~s o1 2/3 7.0 4/5 6.3 _77_ TOXICITY DATA
The hydrochloride salt of the compound of Synthetic Examples 2/3 was administered orally by gavage to Wistar rats as a solution in distilled water at dosages at 25, 100 and 200mg/kg base and to Beagle dogs at dosages of 0.25, 0.50, 1.0 and 2.Omg/kg base once a day for 14 days.
In a separate dog study over 30 days, the dosage of the free base was increased from 2 mg/kg on Day 1 to 100mg/kg on Day 30. The free base was also administered orally to cynomolgus monkeys at a dosage of 50mg/kg once a day for 15 days.
No evidence of toxicity was observed in any of the aforementioned studies at any of the dosages used.

Claims

1. A compound of formula (I) wherein n is 1;
W is a group of formula:
wherein R is hydrogen; X is -O-; Y is oxygen and the chiral centre * in W is in its (S) or (R) form or is a mixture thereof in any proportions; and Z is a group of formula:

wherein R1 and R2 are each methyl, or a physiologically acceptable salt or solvate thereof.

2. A compound of formula (I), as defined in claim 1, which compound is N,N-dimethyl-2-[5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl]ethylamine, in its (R) form, or a physiologically acceptable salt or solvate thereof.

3. (S)-N,N-Dimethyl-2-[5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl]ethylamine or a physiologically acceptable salt or solvate thereof.

4. (S)-N,N-Dimethyl-2-[5-(2-oxo-1,3-oxazolidin-4-ylmethyl)-1H-indol-3-yl]ethylamine.

5. A physiologically acceptable salt of said ethylamine of claim 4.

6. A physiologically acceptable solvate of said ethylamine of claim 4.

7. A compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, for use in a therapeutic agent.

8. A compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, for use in the prophylaxis or treatment of a clinical condition for which a 5-hydroxytryptamine-like receptor agonist is indicated.

9. A compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, for use in the prophylaxis or treatment of migraine.

10. Use of a compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, in the manufacture of a medicament for the prophylaxis or treatment of a clinical condition for which a 5-hydroxytryptamine-like receptor agonist is indicated.

11. Use of a compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, in the manufacture of a medicament for the prophylaxis or treatment of migraine.

12. Use of a compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof as a 5-hydroxytryptamine-like agonist.

13. Use of a compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof as an anti-migraine agent.

14. A compound, salt or solvate of claim 3, 4, 5 or 6, for use as a therapeutic agent.

15. A compound, salt or solvate of claim 3, 4, 5 or 6, for use in the prophylaxis or treatment of a clinical condition for which a 5-hydroxytryptamine receptor agonist is indicated.

16. A compound, salt or solvate of claim 3, 4, 5 or 6, for use in the prophylaxis or treatment of migraine.

17. Use of a compound, salt or solvate of claim 3, 4, 5 or 6, in the manufacture of a medicament for the prophylaxis or treatment of a clinical condition for which a 5-hydroxytryptamine-like receptor agonist is indicated.

18. Use of a compound, salt or solvate of claim 3, 4, 5 or 6, in the manufacture of a medicament for the prophylaxis or treatment of migraine.

19. Use of a compound, salt or solvate of claim 3, 4, 5 or 6, as a 5-hydroxytryptamine-like agonist.

20. Use of a compound, salt or solvate of claim 3, 4, 5 or 6, as an anti-migraine agent.

21. A pharmaceutical composition comprising a compound of formula (I), as claimed in claim 1 or 2, or a physiologically acceptable salt or solvate thereof and a pharmaceutically acceptable carrier.

22. A pharmaceutical composition comprising a physiologically effective, acceptable amount of a compound, salt or solvate of claim 3, 4, 5 or 6, in association with a pharmaceutically acceptable carrier.

23. A 5-hydroxytryptamine-like agonist pharmaceutical composition comprising an acceptable, 5-hydroxytryptamine-like agonistic amount of a compound of formula (I), as defined in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, in association with a pharmaceutically acceptable carrier.

24. An anti-migraine pharmaceutical composition comprising an acceptable anti-migraine effective amount of a compound of formula (I), as defined in claim 1 or 2, or a physiologically acceptable salt or solvate thereof, in association with a pharmaceutically acceptable carrier.

25. A 5-hydroxytryptamine-like agonist pharmaceutical composition comprising an acceptable 5-hydroxytryptamine-like agonistic amount of a compound, salt or solvate of claim 3, 4, 5 or 6, in association with a pharmaceutically acceptable carrier.

26. An anti-migraine pharmaceutical composition comprising an acceptable anti-migraine effective amount of a compound, salt or solvate of claim 3, 4, 5 or 6, in association with a pharmaceutically acceptable carrier.

27. A pharmaceutical composition as claimed in claim 21, which is in the form of a tablet or capsule.

28. A composition as claimed in claim 22, 23 or 24, in the form of a tablet or capsule.

29. A composition as claimed in claim 25 or 26, in the form of a tablet or capsule.

30. A process for the preparation of a compound of formula (I) wherein n is 1;
W is a group of formula:
wherein R is hydrogen; X is -O-; Y is oxygen and the chiral centre * in W is in its (S) or (R) form or is a mixture thereof in any proportions; and Z is a group of formula:

wherein R1 and R2 are each methyl or a physiologically acceptable salt or solvate thereof, which comprises:
(a) reacting a compound of formula (II) wherein n and W are as hereinbefore defined, with a compound of formula (III) or a carbonyl-protected form thereof, wherein L is a leaving group or protected amino group which may be converted in situ to an amine group or is -NR1R2 wherein R1 and R2 are as hereinbefore defined, and where L is a leaving group or a protected amino group, converting L to a group of formula -NR1R2, wherein R1 and R2 are as defined above; or (b) reacting a compound of formula (XV) wherein n, R, Z and X are as hereinbefore defined, with a compound of formula (VII) wherein Y is as hereinbefore defined and L and L', which may be the same or different are leaving groups, and when desired, converting the compound of formula (I) so formed to a corresponding physiologically acceptable salt or solvate thereof.

31. A method of preparing a medicament which comprises:
(a) preparing a compound of formula (I) , as defined in claim 1, or a physiologically acceptable salt or solvate thereof by a process as claimed in claim 30, and (b) admixing the product from step (a) with a pharmaceutically acceptable carrier.

32. A method as claimed in claim 31, which comprises an additional step (c) wherein the admixture from step (b) is formed into a tablet or capsule.