CA2059791A1 - In vitro method to determine the safety of modified hemoglobin blood substitutes for human prior to clinical use - Google Patents
In vitro method to determine the safety of modified hemoglobin blood substitutes for human prior to clinical useInfo
- Publication number
- CA2059791A1 CA2059791A1 CA 2059791 CA2059791A CA2059791A1 CA 2059791 A1 CA2059791 A1 CA 2059791A1 CA 2059791 CA2059791 CA 2059791 CA 2059791 A CA2059791 A CA 2059791A CA 2059791 A1 CA2059791 A1 CA 2059791A1
- Authority
- CA
- Canada
- Prior art keywords
- modified hemoglobin
- blood substitutes
- safety
- hemoglobin blood
- complement activation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to an in vitro method to determine the safety of modified hemoglobin blood substitutes for human subjects prior to their clinical usages, wherein the method is based on complement activation reaction from adding modified hemoglobin blood substitutes to a human plasma sample and comprises the steps of: a) obtaining at least one plasma sample from at least one human subject by i) taking a blood sample and immediately centrifugating; and ii) separating the centrifuged blood sample of step i) and retaining the supernatant plasma; b) mixing the plasma of step ii) with the modified hemoglobin blood substitutes or control-ringer in a weight/volume ratio of about 4:1; c) incubating for a time sufficient to allow for a complement activation reaction to occur; d) adding the product of step c) to an appropriate volume of saline in an EDTA tube; and e) analyzing the degree of complement activation by analysis of the product of step d); thereby determining the safety of the modified hemoglobin blood substitutes relative to the human subject plasma sample based on the detection of complement activation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2059791 CA2059791C (en) | 1992-01-22 | 1992-01-22 | In vitro method to determine the safety of modified hemoglobin blood substitutes for human prior to clinical use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2059791 CA2059791C (en) | 1992-01-22 | 1992-01-22 | In vitro method to determine the safety of modified hemoglobin blood substitutes for human prior to clinical use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2059791A1 true CA2059791A1 (en) | 1993-07-23 |
| CA2059791C CA2059791C (en) | 2004-01-13 |
Family
ID=4149129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2059791 Expired - Fee Related CA2059791C (en) | 1992-01-22 | 1992-01-22 | In vitro method to determine the safety of modified hemoglobin blood substitutes for human prior to clinical use |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2059791C (en) |
-
1992
- 1992-01-22 CA CA 2059791 patent/CA2059791C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2059791C (en) | 2004-01-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |