CA2046802C - Compositions containing protease produced by vibrio and method of use in debridement and wound healing - Google Patents

Compositions containing protease produced by vibrio and method of use in debridement and wound healing Download PDF

Info

Publication number
CA2046802C
CA2046802C CA002046802A CA2046802A CA2046802C CA 2046802 C CA2046802 C CA 2046802C CA 002046802 A CA002046802 A CA 002046802A CA 2046802 A CA2046802 A CA 2046802A CA 2046802 C CA2046802 C CA 2046802C
Authority
CA
Canada
Prior art keywords
protease
composition
wound
vibrio
proteases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002046802A
Other languages
English (en)
French (fr)
Other versions
CA2046802A1 (en
Inventor
Donald Zane Fortney
Donald Richard Durham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WR Grace and Co Conn
Original Assignee
WR Grace and Co Conn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/567,884 external-priority patent/US5145681A/en
Application filed by WR Grace and Co Conn filed Critical WR Grace and Co Conn
Publication of CA2046802A1 publication Critical patent/CA2046802A1/en
Application granted granted Critical
Publication of CA2046802C publication Critical patent/CA2046802C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/63Vibrio
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/909Vibrio

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
CA002046802A 1990-08-15 1991-07-11 Compositions containing protease produced by vibrio and method of use in debridement and wound healing Expired - Lifetime CA2046802C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/567,884 US5145681A (en) 1990-08-15 1990-08-15 Compositions containing protease produced by vibrio and method of use in debridement and wound healing
US567,884 1990-08-15
US67061291A 1991-03-13 1991-03-13
US670,612 1991-03-13

Publications (2)

Publication Number Publication Date
CA2046802A1 CA2046802A1 (en) 1992-02-16
CA2046802C true CA2046802C (en) 2007-05-22

Family

ID=27074608

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002046802A Expired - Lifetime CA2046802C (en) 1990-08-15 1991-07-11 Compositions containing protease produced by vibrio and method of use in debridement and wound healing

Country Status (10)

Country Link
US (1) US5505943A (da)
EP (1) EP0472011B1 (da)
JP (1) JP3253108B2 (da)
AT (1) ATE145139T1 (da)
CA (1) CA2046802C (da)
DE (1) DE69123099T2 (da)
DK (1) DK0472011T3 (da)
ES (1) ES2094171T3 (da)
GR (1) GR3022412T3 (da)
NZ (1) NZ238954A (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840836A (en) * 1989-02-17 1998-11-24 Bayer Corporation Pancreatic islet cell antigens obtained by molecular cloning
US6811989B1 (en) * 1989-02-17 2004-11-02 Bayer Corporation Pancreatic islet cell antigens obtained by molecular cloning
US5468505A (en) * 1992-02-28 1995-11-21 Board Of Regents, The University Of Texas System Local delivery of fibrinolysis enhancing agents
EP0661293A3 (en) * 1993-12-29 1999-05-19 Sanwa Kagaku Kenkyusho Co., Ltd. Protein having cell growth-stimulating and macrophage chemotactic actions, preparative method therefor and use thereof
GB9502120D0 (en) * 1995-02-03 1995-03-22 Chiroscience Ltd Purified enzyme and its preparation
US6017531A (en) * 1997-06-02 2000-01-25 W. R. Grace & Co. Hydrophilic composition containing protease produced by Vibrio
US7184963B1 (en) 1999-01-19 2007-02-27 Bristol-Myers Squibb Company Method for determining care and prevention pathways for clinical management of wounds
NZ513074A (en) * 1999-01-19 2003-11-28 Bristol Myers Squibb Co Method for determining care and prevention pathways for clinical management of wounds
US20040110669A1 (en) * 2001-05-16 2004-06-10 Kakkis Emil D. Destruction of prions using vibriolysin or variants thereof
US20030180281A1 (en) * 2002-03-11 2003-09-25 Bott Richard R. Preparations for topical skin use and treatment
US7223386B2 (en) * 2002-03-11 2007-05-29 Dow Corning Corporation Preparations for topical skin use and treatment
US20030198631A1 (en) * 2002-04-18 2003-10-23 Healthpoint, Ltd. Thermolysin enzymatic wound debrider
US7332179B2 (en) * 2003-12-12 2008-02-19 Kimberly-Clark Worldwide, Inc. Tissue products comprising a cleansing composition
US20050244480A1 (en) * 2004-04-30 2005-11-03 Kimberly-Clark Worldwide, Inc. Pre-wipes for improving anal cleansing
IL165334A0 (en) 2004-11-22 2006-01-15 Mediwound Ltd Debriding composition from bromelain and methods of producing same
US7642395B2 (en) * 2004-12-28 2010-01-05 Kimberly-Clark Worldwide, Inc. Composition and wipe for reducing viscosity of viscoelastic bodily fluids
US20060140899A1 (en) * 2004-12-28 2006-06-29 Kimberly-Clark Worldwide, Inc. Skin cleansing system comprising an anti-adherent formulation and a cationic compound
KR101503958B1 (ko) * 2007-08-31 2015-03-18 디에스엠 아이피 어셋츠 비.브이. 미용 및/또는 피부과학적 용도의 4-아미디노 벤질아민
US8357655B2 (en) * 2008-01-08 2013-01-22 Tufts University Wound healing peptides and methods of use thereof
DE202017000266U1 (de) 2017-01-18 2017-03-29 Norbert Neubauer Debridement - Vorrichtung zur Wundheilung
DE202018002112U1 (de) 2018-04-26 2018-05-25 Norbert Neubauer Wund-Spül-Saugstab

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3409719A (en) * 1967-05-23 1968-11-05 Baxter Laboratories Inc Debridement agent
US3677900A (en) * 1970-05-04 1972-07-18 Univ Lehigh Method of producing collagenase with a species of vibrio
US4329430A (en) * 1979-06-04 1982-05-11 Klein Gerold K V Enzyme mixture
US4276281A (en) * 1980-09-04 1981-06-30 Crikelair George F Burn treatment
US4668228A (en) * 1985-03-12 1987-05-26 Johnson & Johnson Products, Inc. Debriding tape
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene
US4865983A (en) * 1987-12-04 1989-09-12 W. R. Grace & Co.-Conn. Cleaning compositions containing protease produced by vibrio and method of use
US5145681A (en) * 1990-08-15 1992-09-08 W. R. Grace & Co.-Conn. Compositions containing protease produced by vibrio and method of use in debridement and wound healing
EP0498532A1 (en) * 1991-01-10 1992-08-12 E.R. SQUIBB & SONS, INC. Necrotic tissue debridement powder composition containing a proteolytic enzyme

Also Published As

Publication number Publication date
ES2094171T3 (es) 1997-01-16
EP0472011A1 (en) 1992-02-26
ATE145139T1 (de) 1996-11-15
EP0472011B1 (en) 1996-11-13
NZ238954A (en) 1993-09-27
DK0472011T3 (da) 1996-12-02
US5505943A (en) 1996-04-09
GR3022412T3 (en) 1997-04-30
JPH05178761A (ja) 1993-07-20
JP3253108B2 (ja) 2002-02-04
CA2046802A1 (en) 1992-02-16
DE69123099D1 (de) 1996-12-19
DE69123099T2 (de) 1997-03-06

Similar Documents

Publication Publication Date Title
CA2046802C (en) Compositions containing protease produced by vibrio and method of use in debridement and wound healing
US4732758A (en) Preparation with collagenolytic activity having high activity and pharmaceutical compositions containing it
Werb et al. Elastases and elastin degradation
JP5006803B2 (ja) クロストリジウム・バクテリアの培地およびクロストリジウム毒素を得るための方法
US5145681A (en) Compositions containing protease produced by vibrio and method of use in debridement and wound healing
US6638503B2 (en) Streptomyces megasporus sd5, process for the isolation thereof, novel fibrinolytic enzyme prepared therefrom, process for the production of said enzyme and method of treatment of thrombolytic disorders using said enzyme
NZ518059A (en) Topical treatment of streptococcal infections using a lysin enzyme coded for by a C1 bacteriophage
WO1996024371A1 (en) Multifunctional enzyme
US10597632B2 (en) Animal product-free culture medium for bacteria of the genus Clostridium and a process for producing supernatant comprising one or more collagenolytic and gelatinolytic proteases
CN100376679C (zh) 一种蛋白酶,其基因和其用途
US5945102A (en) Crustacean and fish derived multifunctional enzyme
US4329430A (en) Enzyme mixture
EP0988374B1 (en) Hydrophilic composition containing protease produced by vibrio proteolyticus
US4307081A (en) Enzyme mixture
Durham et al. Preliminary evaluation of vibriolysin, a novel proteolytic enzyme composition suitable for the debridement of burn wound eschar
Petrova et al. Novel thermostable serine collagenase from Thermoactinomyces sp. 21E: purification and some properties
JPH05219942A (ja) 細菌クロストリジウム・ヒストリチカムの変異株、その製造法及びその用途
JP3550409B2 (ja) ジクチオステリウムのジペプチジルアミノペプチダーゼ
JP3980088B2 (ja) 傷治癒を改善するプラスミノーゲン活性化因子からなる製剤の使用
Shahzad et al. Production of proteases by genetically improved Bacillus subtilis for enhanced skin penetration of antibacterial topical formulation
Sizer Medical applications of microbial enzymes
RU2236460C1 (ru) Способ получения препарата коллагеназы
Liu et al. Isolation of a novel collagenase-producing strain from animal bone wastes and optimization of its enzyme production
JP2985018B2 (ja) 新規微生物
JP3026111B2 (ja) 新規アルカリプロテアーゼ及びその製造方法

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry