CA2043631A1 - Detecting and imaging in biochemical assays using phosphor screens - Google Patents

Detecting and imaging in biochemical assays using phosphor screens

Info

Publication number
CA2043631A1
CA2043631A1 CA2043631A CA2043631A CA2043631A1 CA 2043631 A1 CA2043631 A1 CA 2043631A1 CA 2043631 A CA2043631 A CA 2043631A CA 2043631 A CA2043631 A CA 2043631A CA 2043631 A1 CA2043631 A1 CA 2043631A1
Authority
CA
Canada
Prior art keywords
imaging
detecting
macromolecules
biochemical assays
phosphor screens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2043631A
Other languages
French (fr)
Other versions
CA2043631C (en
Inventor
George Bers
Franklin R. Witney
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CA2043631A1 publication Critical patent/CA2043631A1/en
Application granted granted Critical
Publication of CA2043631C publication Critical patent/CA2043631C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Immobilized macromolecules such as proteins or nucleic acid sequences are tagged with labels which induce a chemiluminescent reaction in a liquid-phase substrate. As the reaction is occurring, the substrate is exposed to a phosphor screen which absorbs the chemiluminescent emission in an image of the pattern formed by the macromolecules. The absorbed energy is then released and read, providing qualitative and optionally quantitative information regarding the immobilized macromolecules.
CA002043631A 1990-07-12 1991-05-31 Detecting and imaging in biochemical assays using phosphor screens Expired - Fee Related CA2043631C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55196190A 1990-07-12 1990-07-12
US07/551,961 1990-07-12

Publications (2)

Publication Number Publication Date
CA2043631A1 true CA2043631A1 (en) 1992-01-13
CA2043631C CA2043631C (en) 1998-04-21

Family

ID=24203384

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002043631A Expired - Fee Related CA2043631C (en) 1990-07-12 1991-05-31 Detecting and imaging in biochemical assays using phosphor screens

Country Status (7)

Country Link
JP (1) JPH04232864A (en)
CA (1) CA2043631C (en)
DE (1) DE4122839A1 (en)
FR (1) FR2664703B1 (en)
GB (1) GB2246197B (en)
HK (1) HK40795A (en)
IT (1) ITRM910503A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2130947C (en) * 1993-11-12 2001-02-06 Robert E. Emmons Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents
DE4410655C2 (en) * 1994-03-26 2001-06-28 Heiko Schwertner Method and device for fast continuous sequence analysis of various linear polymeric substances according to a uniform procedure
US6415038B1 (en) 1994-05-20 2002-07-02 Fuji Photo Film Co., Ltd. Image analyzing apparatus
JP3571819B2 (en) * 1995-01-09 2004-09-29 富士写真フイルム株式会社 Biochemical image analyzer
JP3550203B2 (en) * 1995-01-10 2004-08-04 富士写真フイルム株式会社 Image analysis device
US5672514A (en) * 1995-02-01 1997-09-30 Fuji Photo Film Co., Ltd. Chemiluminescent detecting method and apparatus
JPH1021373A (en) * 1996-07-05 1998-01-23 Fuji Photo Film Co Ltd Picture analyzing device
JP3851699B2 (en) 1997-02-06 2006-11-29 富士写真フイルム株式会社 Image analysis device
DE19704731B4 (en) * 1997-02-07 2006-07-27 Stratec Biomedical Systems Ag Measuring device for performing luminescence measurements
JP3474765B2 (en) 1998-03-17 2003-12-08 富士写真フイルム株式会社 Image recording and reading system
US6572095B1 (en) 1999-09-03 2003-06-03 Fuji Photo Film Co., Ltd. Method of and system for conveying sheet to be scanned
JP3897272B2 (en) * 1999-09-28 2007-03-22 富士フイルム株式会社 Image analysis device
US6781143B2 (en) * 2001-04-06 2004-08-24 Fuji Photo Film Co., Ltd. Biochemical analysis data producing method, biochemical analysis data producing apparatus and stimulable phosphor sheet used therefor
JP3940995B2 (en) 2002-04-19 2007-07-04 富士フイルム株式会社 Image reading device
JP3913602B2 (en) 2002-04-23 2007-05-09 富士フイルム株式会社 Read image processing method and apparatus

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4396579A (en) * 1981-08-06 1983-08-02 Miles Laboratories, Inc. Luminescence detection device
GB2116709B (en) * 1982-03-09 1986-02-19 Wira & Mather Microbiological testing detecting surface mildew
CA1333776C (en) * 1986-07-22 1995-01-03 Aladar A. Szalay Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
CA1250212A (en) * 1987-04-23 1989-02-21 Merlin M.L. Leong Method and apparatus for instant photodetection of chemiluminescent immunoassays
US4797553A (en) * 1987-05-11 1989-01-10 E. I. Dupont De Nemours And Company Method and apparatus for reading a transparent photostimulable luminescent screen
GB2207245A (en) * 1987-07-15 1989-01-25 Unilever Plc Specific binding chemiluminescent assay
JPH01219563A (en) * 1988-02-27 1989-09-01 Doujin Kagaku Kenkyusho:Kk Detection by photographic method of chemical light emission
GB2233450B (en) * 1989-06-24 1993-06-30 Univ Wales Medicine Detecting or quantifing multiple analytes with luminescent reagents

Also Published As

Publication number Publication date
ITRM910503A0 (en) 1991-07-10
ITRM910503A1 (en) 1992-01-13
GB9115090D0 (en) 1991-08-28
DE4122839A1 (en) 1992-01-16
GB2246197A (en) 1992-01-22
FR2664703A1 (en) 1992-01-17
HK40795A (en) 1995-03-31
GB2246197B (en) 1994-03-16
FR2664703B1 (en) 1995-05-12
CA2043631C (en) 1998-04-21
JPH04232864A (en) 1992-08-21

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Legal Events

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