CA2039482A1 - Method & apparatus for measuring oxygen concentration and its subsequent use in estimating nitrogen fixation in plants - Google Patents
Method & apparatus for measuring oxygen concentration and its subsequent use in estimating nitrogen fixation in plantsInfo
- Publication number
- CA2039482A1 CA2039482A1 CA 2039482 CA2039482A CA2039482A1 CA 2039482 A1 CA2039482 A1 CA 2039482A1 CA 2039482 CA2039482 CA 2039482 CA 2039482 A CA2039482 A CA 2039482A CA 2039482 A1 CA2039482 A1 CA 2039482A1
- Authority
- CA
- Canada
- Prior art keywords
- oxygen concentration
- nodule
- nodules
- plants
- nitrogen fixation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title abstract 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 5
- 239000001301 oxygen Substances 0.000 title abstract 5
- 229910052760 oxygen Inorganic materials 0.000 title abstract 5
- 229910052757 nitrogen Inorganic materials 0.000 title abstract 3
- 108010020943 Nitrogenase Proteins 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 102000001554 Hemoglobins Human genes 0.000 abstract 1
- 108010054147 Hemoglobins Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 238000006213 oxygenation reaction Methods 0.000 abstract 1
- 230000035699 permeability Effects 0.000 abstract 1
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract 1
Landscapes
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A non-invasive method and apparatus are described for measuring the oxygen concentration in the infected cells of the nodules of nitrogen fixing plants in the laboratory or field. In many cases, this information can be used to estimate the nitrogenase activity, and therefore the nitrogen fixation rate, in these nodules since recent studies have shown that the oxygen concentration limits and controls nitrogenase activity under most environmental conditions. Using the same apparatus, a method to measure nodule respiration and nodule oxygen permeability is also described. The nodule oxygen concentration in the infected cells of nodules is maintained at very low levels and has not been measurable previously by non-invasive techniques.
The fractional oxygenation of plant hemoglobin is measured spectroscopically using one or more modulated light sources, a number of optical fibres to convey the light to and from the nodules, a photodetector to measure the light passing through the nodule and a detection system such as a photodiode coupled to a microcomputer or a lock-in amplifier to process the output signal.
The fractional oxygenation of plant hemoglobin is measured spectroscopically using one or more modulated light sources, a number of optical fibres to convey the light to and from the nodules, a photodetector to measure the light passing through the nodule and a detection system such as a photodiode coupled to a microcomputer or a lock-in amplifier to process the output signal.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US49573290A | 1990-03-19 | 1990-03-19 | |
US495,732 | 1990-03-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2039482A1 true CA2039482A1 (en) | 1991-09-20 |
CA2039482C CA2039482C (en) | 2001-02-13 |
Family
ID=23969793
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2039482 Expired - Fee Related CA2039482C (en) | 1990-03-19 | 1991-03-18 | Method & apparatus for measuring oxygen concentration and its subsequent use in estimating nitrogen fixation in plants |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2039482C (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104849219B (en) * | 2015-03-11 | 2017-09-26 | 中国科学院地理科学与资源研究所 | A kind of crop nitrogen nutrition diagnostic equipment and method |
-
1991
- 1991-03-18 CA CA 2039482 patent/CA2039482C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2039482C (en) | 2001-02-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |