CA2000098A1 - Pyruvate oxidase mutants and process for the preparation thereof - Google Patents

Pyruvate oxidase mutants and process for the preparation thereof

Info

Publication number
CA2000098A1
CA2000098A1 CA002000098A CA2000098A CA2000098A1 CA 2000098 A1 CA2000098 A1 CA 2000098A1 CA 002000098 A CA002000098 A CA 002000098A CA 2000098 A CA2000098 A CA 2000098A CA 2000098 A1 CA2000098 A1 CA 2000098A1
Authority
CA
Canada
Prior art keywords
pyruvate
pbp
oxidase
pyruvate oxidase
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002000098A
Other languages
English (en)
French (fr)
Inventor
Gunther Schumacher
Hans Moellering
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of CA2000098A1 publication Critical patent/CA2000098A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/03—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with oxygen as acceptor (1.2.3)
    • C12Y102/03003—Pyruvate oxidase (1.2.3.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002000098A 1988-10-03 1989-10-03 Pyruvate oxidase mutants and process for the preparation thereof Abandoned CA2000098A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3833601.4 1988-10-03
DE3833601A DE3833601A1 (de) 1988-10-03 1988-10-03 Pyruvatoxidase-mutanten

Publications (1)

Publication Number Publication Date
CA2000098A1 true CA2000098A1 (en) 1990-04-03

Family

ID=6364285

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002000098A Abandoned CA2000098A1 (en) 1988-10-03 1989-10-03 Pyruvate oxidase mutants and process for the preparation thereof

Country Status (6)

Country Link
US (1) US5096821A (OSRAM)
EP (1) EP0365836A1 (OSRAM)
JP (1) JPH02177884A (OSRAM)
AU (1) AU617535B2 (OSRAM)
CA (1) CA2000098A1 (OSRAM)
DE (1) DE3833601A1 (OSRAM)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3306719A1 (de) * 1983-02-25 1984-08-30 Boehringer Mannheim Gmbh, 6800 Mannheim Pyruvatoxidase
DE3886225T2 (de) * 1987-01-06 1994-03-31 Asahi Chemical Ind Pyruvat-Oxidase, ihre Herstellung und Verwendung.

Also Published As

Publication number Publication date
US5096821A (en) 1992-03-17
EP0365836A1 (de) 1990-05-02
AU617535B2 (en) 1991-11-28
JPH02177884A (ja) 1990-07-10
AU4240489A (en) 1990-04-05
JPH0544272B2 (OSRAM) 1993-07-05
DE3833601A1 (de) 1990-04-05

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued