CA1318624C - Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides - Google Patents

Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides

Info

Publication number
CA1318624C
CA1318624C CA000611641A CA611641A CA1318624C CA 1318624 C CA1318624 C CA 1318624C CA 000611641 A CA000611641 A CA 000611641A CA 611641 A CA611641 A CA 611641A CA 1318624 C CA1318624 C CA 1318624C
Authority
CA
Canada
Prior art keywords
concentration
glutaraldehyde
cross
lipase
lipase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA000611641A
Other languages
English (en)
Inventor
Gerald Andre
Michael Trani
Francoise Ergan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Priority to CA000611641A priority Critical patent/CA1318624C/fr
Application granted granted Critical
Publication of CA1318624C publication Critical patent/CA1318624C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA000611641A 1989-09-15 1989-09-15 Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides Expired - Fee Related CA1318624C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA000611641A CA1318624C (fr) 1989-09-15 1989-09-15 Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA000611641A CA1318624C (fr) 1989-09-15 1989-09-15 Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides

Publications (1)

Publication Number Publication Date
CA1318624C true CA1318624C (fr) 1993-06-01

Family

ID=4140606

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000611641A Expired - Fee Related CA1318624C (fr) 1989-09-15 1989-09-15 Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides

Country Status (1)

Country Link
CA (1) CA1318624C (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118652945A (zh) * 2024-08-19 2024-09-17 广东善百年特医食品有限公司 一种复合酶法生产甘油二酯的工艺

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118652945A (zh) * 2024-08-19 2024-09-17 广东善百年特医食品有限公司 一种复合酶法生产甘油二酯的工艺

Similar Documents

Publication Publication Date Title
EP0746608B1 (fr) Procede pour la production d'une preparation enzymatique immobilisee et utilisation de cette preparation
US6596520B1 (en) Immobilizing lipase by adsorption from a crude solution onto nonpolar polyolefin particles
US6605452B1 (en) Fatty acid polyol ester-coated lipase complex immobilized on insoluble matrix
AU637113B2 (en) Enzymes and their use
KR100351887B1 (ko) 고정화리파아제
JP2873251B2 (ja) 粒状固定化リパーゼ調製品、その製法およびその使用
Wisdom et al. Enzymic interesterification of fats: Laboratory and pilot‐scale studies with immobilized lipase from Rhizopus arrhizus
Otero et al. Immobilization/stabilization of lipase from Candida rugosa
EP1163329A2 (fr) Complexe lipase-tensioactif immobilise sur une matrice insoluble
US5316927A (en) Production of monoglycerides by enzymatic transesterification
De La Casa et al. Modification of the activities of two different lipases from Candida rugosa with dextrans
Basri et al. Immobilization of hydrophobic lipase derivatives on to organic polymer beads
Basri et al. Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters
Hsu et al. Immobilization of lipoxygenase in an alginate-silicate solgel matrix: formation of fatty acid hydroperoxides
CA2000143A1 (fr) Production de monoglycerides par transesterification enzymatique
US5010006A (en) Lipase immdoilized by cross-linking with inert protein for glyceride synthesis
US5508182A (en) Esterification of hydrophilic polyols by adsorption onto a solid support and employing a substrate-immiscible solvent
JPH04287689A (ja) 固定化酵素によるアルコールのアシル化方法
CA1318624C (fr) Preparation de lipases immobilisees et leur utilisation dans la synthese de glycerides
Kaga et al. Stabilization of Candida lipase against acetaldehyde by adsorption onto celite
Kaimal et al. Enhancement of catalytic activity of porcine pancreatic lipase by reductive alkylation
AU729928B2 (en) Immobilized esterases from crude extract and their use
Tahoun Large agarose-lipase beads for the hydrolysis of triglycerides
Kanwar et al. Enhancement of ethyl propionate synthesis by poly (AAc-co-HPMA-cl-MBAm)-immobilized pseudomonas aeruginosa MTCC-4713, exposed to Hg 2+ and NH 4+ ions
NL1017258C2 (nl) Werkwijze voor het maken van vernette enzymaggregaten.

Legal Events

Date Code Title Description
MKLA Lapsed