CA1288338C - Method and kit for diagnosing plant diseases - Google Patents
Method and kit for diagnosing plant diseasesInfo
- Publication number
- CA1288338C CA1288338C CA000537468A CA537468A CA1288338C CA 1288338 C CA1288338 C CA 1288338C CA 000537468 A CA000537468 A CA 000537468A CA 537468 A CA537468 A CA 537468A CA 1288338 C CA1288338 C CA 1288338C
- Authority
- CA
- Canada
- Prior art keywords
- liquid solution
- antigen
- antibody
- extraction pad
- carrier body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 29
- 201000010099 disease Diseases 0.000 title description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title description 14
- 239000000427 antigen Substances 0.000 claims abstract description 65
- 102000036639 antigens Human genes 0.000 claims abstract description 65
- 108091007433 antigens Proteins 0.000 claims abstract description 65
- 239000006193 liquid solution Substances 0.000 claims abstract description 45
- 238000012360 testing method Methods 0.000 claims abstract description 26
- 238000000605 extraction Methods 0.000 claims description 58
- 239000012528 membrane Substances 0.000 claims description 30
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 238000012546 transfer Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 39
- 241000196324 Embryophyta Species 0.000 description 30
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 239000007787 solid Substances 0.000 description 5
- 244000025254 Cannabis sativa Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 238000002405 diagnostic procedure Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000002981 blocking agent Substances 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 239000000123 paper Substances 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 2
- 240000001987 Pyrus communis Species 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 102000005936 beta-Galactosidase Human genes 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 239000007975 buffered saline Substances 0.000 description 2
- 229910001651 emery Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 1
- 241001317374 Evides Species 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920006328 Styrofoam Polymers 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 239000008261 styrofoam Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Testing And Monitoring For Control Systems (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/865,379 US5047207A (en) | 1986-05-20 | 1986-05-20 | Kit for diagnosing plant diseases |
| US865,379 | 1992-04-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1288338C true CA1288338C (en) | 1991-09-03 |
Family
ID=25345378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000537468A Expired - Lifetime CA1288338C (en) | 1986-05-20 | 1987-05-20 | Method and kit for diagnosing plant diseases |
Country Status (8)
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0246643A3 (en) * | 1986-05-20 | 1988-06-01 | DNA PLANT TECHNOLOGY OPERATING CO. (under the laws of the State of Delaware) | Method of preparing diagnostic test dipsticks |
| ES2099147T3 (es) * | 1990-06-29 | 1997-05-16 | Ciba Geigy Ag | Anticuerpos monoclonales de las especies mycosphaerella. |
| US5789183A (en) * | 1992-08-14 | 1998-08-04 | University Of Arkansas | Serological detection and identification of rice blast |
| US5419209A (en) * | 1993-03-15 | 1995-05-30 | Environmental Realty Guild Of America, Inc. | System and protocol for residential environmental assessment |
| US5563040A (en) * | 1994-03-21 | 1996-10-08 | The United States Of America As Represented By The Secretary Of Agriculture | Method and apparatus for immunological diagnosis of fungal decay in wood |
| US7337684B1 (en) * | 2005-05-09 | 2008-03-04 | Lewis Nicholas J | Tool for removing surface material |
| CN115402629B (zh) * | 2022-09-30 | 2024-07-19 | 重庆医科大学 | 一种胰腺癌的病理研究用试剂盒 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US847190A (en) * | 1905-06-16 | 1907-03-12 | Ezra F Landis | Abrading material. |
| US875936A (en) * | 1907-07-30 | 1908-01-07 | Ezra F Landis | Abrading material. |
| US2985288A (en) * | 1959-08-10 | 1961-05-23 | Stanley C Schaffer | Diagnostic package |
| USRE29725E (en) * | 1966-04-26 | 1978-08-08 | E. I. Du Pont De Nemours And Company | Analytical test pack and process for analysis |
| US3469958A (en) * | 1966-12-05 | 1969-09-30 | Abrasive Products Inc | Dimensionally stable flexible abrasive sheet material |
| BE795221A (fr) * | 1972-02-12 | 1973-08-09 | Merck Patent Gmbh | Procede et reactif rapide en vue de deceler des stupefiants |
| US3890204A (en) * | 1974-09-26 | 1975-06-17 | Marion Health And Safety Inc | Culture collecting package |
| US3965888A (en) * | 1975-02-12 | 1976-06-29 | Brenner And Bender, Inc. | Specimen collector and holder |
| CA1048390A (en) * | 1975-02-14 | 1979-02-13 | James B. Dugle | Method, composition, and device for determining the specific gravity of a liquid |
| AT370312B (de) * | 1978-08-10 | 1983-03-25 | Roehm Gmbh | Testbriefe fuer stuhlproben |
| US4244940A (en) * | 1978-09-05 | 1981-01-13 | Bio-Rad Laboratories, Inc. | Single-incubation two-site immunoassay |
| US4259964A (en) * | 1979-07-09 | 1981-04-07 | Levine Robert A | Device for obtaining stool samples |
| US4273741A (en) * | 1979-07-09 | 1981-06-16 | Levine Robert A | Device for obtaining stool samples |
| US4305924A (en) * | 1979-08-08 | 1981-12-15 | Ventrex Laboratories, Inc. | Method and apparatus for performing in vitro clinical diagnostic tests using a solid phase assay system |
| US4420353A (en) * | 1980-01-07 | 1983-12-13 | Levine Robert A | Method of making a stool sampling device |
| US4303610A (en) * | 1980-05-19 | 1981-12-01 | Pennzoil Company | Test kit for field analysis of plant tissue magnesium and calcium |
| JPS5745454A (en) * | 1980-09-02 | 1982-03-15 | Fuji Photo Film Co Ltd | Immunochemical measuring method for various minor components |
| US4471058A (en) * | 1982-07-26 | 1984-09-11 | Board Of Trustees Operating Michigan State University | Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies |
| EP0113075A3 (en) * | 1982-12-06 | 1985-05-02 | Fielder Gillespie Davis Limited | Field immunoassay reaction system, method of immunoassay diagnosis and probe or carrier for use therewith |
| DE3323973A1 (de) * | 1983-07-02 | 1985-01-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | Erythrozyten-rueckhaltesubstrate |
| US4605404A (en) * | 1985-01-16 | 1986-08-12 | Sneider Vincent R | Disposable pad for panty hose |
| US4845197A (en) * | 1985-09-09 | 1989-07-04 | Agri-Diagnostics Associates | Monoclonal antibodies and methods for fungal pathogen detection |
| EP0246643A3 (en) * | 1986-05-20 | 1988-06-01 | DNA PLANT TECHNOLOGY OPERATING CO. (under the laws of the State of Delaware) | Method of preparing diagnostic test dipsticks |
-
1986
- 1986-05-20 US US06/865,379 patent/US5047207A/en not_active Expired - Fee Related
-
1987
- 1987-05-19 DE DE8787107274T patent/DE3781323T2/de not_active Expired - Lifetime
- 1987-05-19 AT AT87107274T patent/ATE79955T1/de not_active IP Right Cessation
- 1987-05-19 EP EP87107274A patent/EP0246615B1/en not_active Expired - Lifetime
- 1987-05-19 ES ES87107274T patent/ES2043620T3/es not_active Expired - Lifetime
- 1987-05-20 CA CA000537468A patent/CA1288338C/en not_active Expired - Lifetime
- 1987-05-20 JP JP62123584A patent/JPS62284261A/ja active Pending
-
1992
- 1992-09-17 GR GR920402052T patent/GR3005729T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US5047207A (en) | 1991-09-10 |
| DE3781323D1 (de) | 1992-10-01 |
| ATE79955T1 (de) | 1992-09-15 |
| EP0246615A3 (en) | 1989-01-25 |
| EP0246615A2 (en) | 1987-11-25 |
| DE3781323T2 (de) | 1992-12-17 |
| ES2043620T3 (es) | 1994-01-01 |
| JPS62284261A (ja) | 1987-12-10 |
| GR3005729T3 (US08168396-20120501-C00001.png) | 1993-06-07 |
| EP0246615B1 (en) | 1992-08-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed | ||
| MKLA | Lapsed |
Effective date: 19980903 |