CA1237984A - Antigene viral sur un support, preparation et application - Google Patents
Antigene viral sur un support, preparation et applicationInfo
- Publication number
- CA1237984A CA1237984A CA000529953A CA529953A CA1237984A CA 1237984 A CA1237984 A CA 1237984A CA 000529953 A CA000529953 A CA 000529953A CA 529953 A CA529953 A CA 529953A CA 1237984 A CA1237984 A CA 1237984A
- Authority
- CA
- Canada
- Prior art keywords
- virus
- rubella
- antigen
- rubella virus
- sensitized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000427 antigen Substances 0.000 title abstract description 77
- 102000036639 antigens Human genes 0.000 title abstract description 77
- 108091007433 antigens Proteins 0.000 title abstract description 77
- 230000003612 virological effect Effects 0.000 title abstract description 9
- 238000002360 preparation method Methods 0.000 title abstract description 3
- 241000710799 Rubella virus Species 0.000 claims abstract description 95
- 241000700605 Viruses Species 0.000 claims description 73
- 238000000034 method Methods 0.000 claims description 36
- 238000001179 sorption measurement Methods 0.000 claims description 16
- 108010067390 Viral Proteins Proteins 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 7
- 230000006872 improvement Effects 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 3
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 3
- 229940085991 phosphate ion Drugs 0.000 claims description 3
- 239000007787 solid Substances 0.000 abstract description 25
- 238000003556 assay Methods 0.000 abstract description 19
- 230000007928 solubilization Effects 0.000 abstract description 12
- 238000005063 solubilization Methods 0.000 abstract description 12
- 239000002245 particle Substances 0.000 description 38
- 201000005404 rubella Diseases 0.000 description 30
- 102000004169 proteins and genes Human genes 0.000 description 21
- 108090000623 proteins and genes Proteins 0.000 description 20
- 230000004520 agglutination Effects 0.000 description 14
- 210000002966 serum Anatomy 0.000 description 13
- 239000004816 latex Substances 0.000 description 12
- 229920000126 latex Polymers 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 239000004793 Polystyrene Substances 0.000 description 11
- 101710172711 Structural protein Proteins 0.000 description 11
- 238000007818 agglutination assay Methods 0.000 description 11
- 229920002223 polystyrene Polymers 0.000 description 11
- 239000004094 surface-active agent Substances 0.000 description 10
- 239000000872 buffer Substances 0.000 description 8
- 239000003599 detergent Substances 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 239000011248 coating agent Substances 0.000 description 7
- 238000000576 coating method Methods 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 230000000890 antigenic effect Effects 0.000 description 6
- 230000004888 barrier function Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000007822 coupling agent Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- 201000006747 infectious mononucleosis Diseases 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- -1 alkali metal salts Chemical class 0.000 description 3
- 230000036528 appetite Effects 0.000 description 3
- 235000019789 appetite Nutrition 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 238000012207 quantitative assay Methods 0.000 description 3
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000282320 Panthera leo Species 0.000 description 2
- 241000207961 Sesamum Species 0.000 description 2
- 235000003434 Sesamum indicum Nutrition 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229920001059 synthetic polymer Polymers 0.000 description 2
- GMLDCZYTIPCVMO-UHFFFAOYSA-N 2-methylidenebutanal Chemical compound CCC(=C)C=O GMLDCZYTIPCVMO-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 241000282552 Chlorocebus aethiops Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 101710154606 Hemagglutinin Proteins 0.000 description 1
- 208000009889 Herpes Simplex Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 208000037062 Polyps Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 101710176177 Protein A56 Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 241001111950 Sonora Species 0.000 description 1
- PCSMJKASWLYICJ-UHFFFAOYSA-N Succinic aldehyde Chemical compound O=CCCC=O PCSMJKASWLYICJ-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- MLUCVPSAIODCQM-NSCUHMNNSA-N crotonaldehyde Chemical compound C\C=C\C=O MLUCVPSAIODCQM-NSCUHMNNSA-N 0.000 description 1
- MLUCVPSAIODCQM-UHFFFAOYSA-N crotonaldehyde Natural products CC=CC=O MLUCVPSAIODCQM-UHFFFAOYSA-N 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- RAABOESOVLLHRU-UHFFFAOYSA-N diazene Chemical compound N=N RAABOESOVLLHRU-UHFFFAOYSA-N 0.000 description 1
- 229910000071 diazene Inorganic materials 0.000 description 1
- 125000005442 diisocyanate group Chemical group 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 239000000185 hemagglutinin Substances 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000012092 latex agglutination test Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 150000001247 metal acetylides Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000012205 qualitative assay Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000529953A CA1237984A (fr) | 1984-03-13 | 1987-02-11 | Antigene viral sur un support, preparation et application |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000449487A CA1231305A (fr) | 1984-03-13 | 1984-03-13 | Antigene viral sur un support, mode de preparation et application |
| CA000529953A CA1237984A (fr) | 1984-03-13 | 1987-02-11 | Antigene viral sur un support, preparation et application |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000449487A Division CA1231305A (fr) | 1984-03-13 | 1984-03-13 | Antigene viral sur un support, mode de preparation et application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1237984A true CA1237984A (fr) | 1988-06-14 |
Family
ID=4127394
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000449487A Expired CA1231305A (fr) | 1984-03-13 | 1984-03-13 | Antigene viral sur un support, mode de preparation et application |
| CA000529953A Expired CA1237984A (fr) | 1984-03-13 | 1987-02-11 | Antigene viral sur un support, preparation et application |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000449487A Expired CA1231305A (fr) | 1984-03-13 | 1984-03-13 | Antigene viral sur un support, mode de preparation et application |
Country Status (1)
| Country | Link |
|---|---|
| CA (2) | CA1231305A (fr) |
-
1984
- 1984-03-13 CA CA000449487A patent/CA1231305A/fr not_active Expired
-
1987
- 1987-02-11 CA CA000529953A patent/CA1237984A/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CA1231305A (fr) | 1988-01-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4657873A (en) | Preactivated plastics surfaces for immobilizing organo-chemical and biologic materials | |
| US4069352A (en) | Immunoadsorbent polymeric material and method of making same | |
| US4347311A (en) | Enzyme immunoassay for determining antigen specific antibodies and test kit for carrying out this assay | |
| Jagendorf et al. | Use of antibody bound to modified cellulose as an immunospecific adsorbent of antigens | |
| Parsons Jr | [14] Antibody-coated plastic tubes in radioimmunoassay | |
| US5506110A (en) | Carrier for binding of anti-phospholipid antibodies, and immunoassay method using the same and a kit therefor | |
| EP0051985B2 (fr) | Essai immunologique de protéines | |
| US4695537A (en) | Particles sensitized with detergent-treated antigen for agglutination immunoassay | |
| AU648625B2 (en) | Test method and reagent kit therefor | |
| US4195074A (en) | Process for producing a soluble rubella antigen | |
| US4590156A (en) | Agglutination assay and product for rubella antibody | |
| US4808518A (en) | Recovery of cytomegalovirus antigen and use thereof in an assay | |
| EP0161328B1 (fr) | Antigène viral appliqué sur support et sa préparation et son usage | |
| US4612281A (en) | Immunoassay for detecting immunoglobulins and test kit | |
| CA1231305A (fr) | Antigene viral sur un support, mode de preparation et application | |
| EP0186946B1 (fr) | Test d'agglutination passif pour les anticorps de la pseudo-rabies | |
| GB1592825A (en) | Rubella virus immunoassay materials and methods for making and using same | |
| EP0413587B1 (fr) | Méthode d'association chimique directe de dimère-D provenant d'un échantillon biologique et permettant le diagnostic et le suivi d'états de thrombolyse et d'hypercoagulabilité | |
| Urano et al. | Influence of anionic compounds on adsorption behavior of bovine serum albumin at oxide-water interfaces | |
| AU624650B2 (en) | One-step immunoassay for determining antigen-specific antibodies of all immunoglobulin classes, and an agent therefor | |
| RU2089912C1 (ru) | Способ стереоспецифического анализа и способ получения конъюгата для стереоспецифического анализа | |
| EP0166224B1 (fr) | Procédé pour essayer l'anticorps du ATL-virus et réactif pour ceci | |
| Shimura et al. | Affinophoresis of red blood cells: Surface antigen‐specific acceleration of electrophoresis | |
| EP0609766B1 (fr) | Procédé de stabilisation de l'antigénéité de myeloperoxidase | |
| JPH0862219A (ja) | 還元型抗原に対する抗体アッセイ試薬及びそれを使用した測定方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |