CA1231634A - Method for the detection of bis (2- chloroethyl)sulfide or bis (2-chloroethyl)imine - Google Patents
Method for the detection of bis (2- chloroethyl)sulfide or bis (2-chloroethyl)imineInfo
- Publication number
- CA1231634A CA1231634A CA000468798A CA468798A CA1231634A CA 1231634 A CA1231634 A CA 1231634A CA 000468798 A CA000468798 A CA 000468798A CA 468798 A CA468798 A CA 468798A CA 1231634 A CA1231634 A CA 1231634A
- Authority
- CA
- Canada
- Prior art keywords
- bis
- dyestuff
- chloroethyl
- adduct
- sulfide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—Specially adapted to detect a particular component
- G01N33/0047—Specially adapted to detect a particular component for organic compounds
- G01N33/0049—Specially adapted to detect a particular component for organic compounds for halogenated organic compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
Abstact For the detection of bis(2-chloroethyl)sulfide or -imine, a dyestuff capable of fluorescing is added, which forms an adduct with the substance to be detected, said adduct being capable of fluorescing in a spectral range different from that of said dyestuff.
Description
~L23~L63~
===== =============.===================
Method for the Detection of bis(2-chloro-!' ethyl sulfide or bist2-chloroethyl)imine ========================================
The invention relates to a method for the detection of bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine, according to which a chemical reagent is applied to ( the surface on which the substance to be detected is assumed to be present and the radiation thus generated is optically determined.
Bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine is the warfare agent LOST and is usually detected on surfaces using indicators contained in paper or powder. LOST is also detected by gas chromatography after extraction from the surface. Moreover, color reactions are known which take place in aqueous or organic solutions. These reactions are carried out in test tubes.
The German Patent No. 29 47 459 describes a device for in-situ detection of precipitates of specific known agents consisting of phosphoric esters. Upon addition of insole, these compounds exhibit a chemical luminescence which can be detected without contact by means of an optical detector. However, this reaction is not applicable to the chemical warfare agent LOST.
The object of this invention is, therefore, to provide a method for the detection of LOST which can be easily realized.
This object can be achieved according to the invention with a method comprising using a distaff being capable , ~23~L~3~
. - 2 -of fluorescing and forming an adduce with bis(2-chloroethyl)-sulfide or bis(2-chloroethyl)lmine, said adduce being capable of fluorescing in a spectral range different from that of said distaff. Preferably, a triphenyl methane distaff, especially neutral red, guinea green B, lissamine green B or brilliant blue R, is used as distaff. These distaffs are applied from aqueous/organic or organic solutions onto the surface on which the warfare agent is assumed to be present. The amount of distaff in the solution is between 0.001 and 0.5 weight percent, preferably between 0.01 and 0.1 weight percent.
( According to the invention, distaffs having a reactive center are used which affect or decompose LOST nucleophilically or electrophilically, or LOST is fixed by adsorption under confirmational change, or LOST is incorporated in an inclusion compound. Thereby, the characteristic fluorescence of the distaff is changed.
The wavelength of the excitation is preferably between 300 and 400 no. The wavelength of the emission is preferably between 380 and 500 no. Mixtures of water and alcohol, water and acetone or water and dim ethyl formamide, or also alcohol, acetone or hexane without addition of water are preferably used as solvents for the distaff. Especially triphenyl methane distaffs are used as chemical reagents.
The invention is explained in more detail in the following examples.
Example 1 Neutral red is dissolved in ethanol in an amount of 10 weight percent. using 5 Al sulfide-LOST, an adduce is formed which, upon excitation with light of the wavelength of 330 no exhibits fluorescence of a relative intensity of 1500 at 392 no.
~3~63~
_ 3 -Example 2 The method of example l is repeated, but in this example Guinea green B is used as distaff. The adduce shows fluorescence of a relative intensity of 11700 at 394 no upon excitation with light of the wavelength of 320 no.
Example 3 Lissamine green B is dissolved in an amount of 10 3 weight percent in a mixture of 70 percent water and 30 percent ethanol. Using S Al LOST an adduce is formed which, upon lo excitation with light of the wavelength of 340 no, shows fluorescence of a relative intensity of 810 at 470 no.
Example 4 Brilliant blue is dissolved in an amount of 2 x 10 2 weight percent in a mixture of 70 percent water and 30 percent ethanol. The adduce obtained, upon excitation with light of the wavelength of 370 no, shows a fluorescence of a relative intensity of 28100 at 455 no.
===== =============.===================
Method for the Detection of bis(2-chloro-!' ethyl sulfide or bist2-chloroethyl)imine ========================================
The invention relates to a method for the detection of bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine, according to which a chemical reagent is applied to ( the surface on which the substance to be detected is assumed to be present and the radiation thus generated is optically determined.
Bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine is the warfare agent LOST and is usually detected on surfaces using indicators contained in paper or powder. LOST is also detected by gas chromatography after extraction from the surface. Moreover, color reactions are known which take place in aqueous or organic solutions. These reactions are carried out in test tubes.
The German Patent No. 29 47 459 describes a device for in-situ detection of precipitates of specific known agents consisting of phosphoric esters. Upon addition of insole, these compounds exhibit a chemical luminescence which can be detected without contact by means of an optical detector. However, this reaction is not applicable to the chemical warfare agent LOST.
The object of this invention is, therefore, to provide a method for the detection of LOST which can be easily realized.
This object can be achieved according to the invention with a method comprising using a distaff being capable , ~23~L~3~
. - 2 -of fluorescing and forming an adduce with bis(2-chloroethyl)-sulfide or bis(2-chloroethyl)lmine, said adduce being capable of fluorescing in a spectral range different from that of said distaff. Preferably, a triphenyl methane distaff, especially neutral red, guinea green B, lissamine green B or brilliant blue R, is used as distaff. These distaffs are applied from aqueous/organic or organic solutions onto the surface on which the warfare agent is assumed to be present. The amount of distaff in the solution is between 0.001 and 0.5 weight percent, preferably between 0.01 and 0.1 weight percent.
( According to the invention, distaffs having a reactive center are used which affect or decompose LOST nucleophilically or electrophilically, or LOST is fixed by adsorption under confirmational change, or LOST is incorporated in an inclusion compound. Thereby, the characteristic fluorescence of the distaff is changed.
The wavelength of the excitation is preferably between 300 and 400 no. The wavelength of the emission is preferably between 380 and 500 no. Mixtures of water and alcohol, water and acetone or water and dim ethyl formamide, or also alcohol, acetone or hexane without addition of water are preferably used as solvents for the distaff. Especially triphenyl methane distaffs are used as chemical reagents.
The invention is explained in more detail in the following examples.
Example 1 Neutral red is dissolved in ethanol in an amount of 10 weight percent. using 5 Al sulfide-LOST, an adduce is formed which, upon excitation with light of the wavelength of 330 no exhibits fluorescence of a relative intensity of 1500 at 392 no.
~3~63~
_ 3 -Example 2 The method of example l is repeated, but in this example Guinea green B is used as distaff. The adduce shows fluorescence of a relative intensity of 11700 at 394 no upon excitation with light of the wavelength of 320 no.
Example 3 Lissamine green B is dissolved in an amount of 10 3 weight percent in a mixture of 70 percent water and 30 percent ethanol. Using S Al LOST an adduce is formed which, upon lo excitation with light of the wavelength of 340 no, shows fluorescence of a relative intensity of 810 at 470 no.
Example 4 Brilliant blue is dissolved in an amount of 2 x 10 2 weight percent in a mixture of 70 percent water and 30 percent ethanol. The adduce obtained, upon excitation with light of the wavelength of 370 no, shows a fluorescence of a relative intensity of 28100 at 455 no.
Claims (6)
1. A method for the detection of bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine which comprises applying a chemical agent to the surface on which the substance to be detected is assumed to be present and optically determining the radiation generated, wherein said chemical agent is a dyestuff capable of fluorescing and forming an adduct with bis(2-chloroethyl) sulfide or bis(2-chloroethyl)imine, said adduct being capable of fluorescing in a spectral range different from that of said dyestuff.
2. A method as claimed in Claim 1, wherein a triphenyl methine dyestuff is applied as said chemical agent.
3. A method as claimed in Claim 1, wherein a dyestuff selected from the group consisting of neutral red, guinea green B, lissamine green B and brilliant blue R is applied as said chemical agent.
4. A method as claimed in any one of Claims 1 to 3, wherein the dyestuff is applied in aqueous/organic or organic solution containing between 0.5 and 0.001 weight percent dyestuff.
5. A method as claimed in any one of Claims 1 to 3, wherein the dyestuff is applied in aqueous/organic or organic solution containing between 0.1 and 0.01 weight percent dyestuff.
6. A method for the detection of substances bis(2-chloro-ethyl)sulfide or bis(2-chloroethyl)imine comprising:
applying to a surface, on which either substance to be detected is assumed to be present, a chemical agent comprising a dyestuff capable of fluorescing and forming an adduct with bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine, the adduct capable of fluorescing in a spectral range different from that of the dyestuff, exciting the adduct with light so as to induce light emissions by fluorescence, optically determining the wavelength of the light emitted for a difference in the wavelengths of emission between the adduct and dyestuff.
applying to a surface, on which either substance to be detected is assumed to be present, a chemical agent comprising a dyestuff capable of fluorescing and forming an adduct with bis(2-chloroethyl)sulfide or bis(2-chloroethyl)imine, the adduct capable of fluorescing in a spectral range different from that of the dyestuff, exciting the adduct with light so as to induce light emissions by fluorescence, optically determining the wavelength of the light emitted for a difference in the wavelengths of emission between the adduct and dyestuff.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3344700.4 | 1983-12-10 | ||
| DE19833344700 DE3344700A1 (en) | 1983-12-10 | 1983-12-10 | METHOD FOR DETECTING BIS- (2-CHLORETHYL) -SULFIDE OR BIS- (2-CHLORETHYL) -IMINE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1231634A true CA1231634A (en) | 1988-01-19 |
Family
ID=6216585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000468798A Expired CA1231634A (en) | 1983-12-10 | 1984-11-28 | Method for the detection of bis (2- chloroethyl)sulfide or bis (2-chloroethyl)imine |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0164379B1 (en) |
| JP (1) | JPS61500687A (en) |
| AT (1) | ATE37607T1 (en) |
| CA (1) | CA1231634A (en) |
| DE (1) | DE3344700A1 (en) |
| FI (1) | FI75056C (en) |
| NO (1) | NO164383C (en) |
| WO (1) | WO1985002679A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3803142A1 (en) * | 1988-02-03 | 1989-08-17 | Battelle Institut E V | METHOD FOR DETECTING A COMBAT SUBSTANCE |
| DE3844796C2 (en) * | 1988-02-03 | 1993-01-21 | Battelle-Institut E.V., 6000 Frankfurt, De | Chemical warfare agent detection with fluorescent reagent |
| GB9419659D0 (en) * | 1994-09-28 | 1994-11-16 | Secr Defence | Detection of sulphur containing compounds |
| US5591400A (en) * | 1994-10-31 | 1997-01-07 | Minnesota Mining And Manufacturing Company | Method for producing an ionic sensor |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE722291C (en) * | 1933-09-28 | 1942-07-07 | Deutsches Reich Vertreten Durc | Process for the detection of splashes of adhesive warfare agents on the ground or on objects |
| CH184663A (en) * | 1935-08-07 | 1936-06-15 | Heinrich Dr Draeger Otto | Method for the detection of dichlorodiethyl sulfide. |
| US3960759A (en) * | 1965-11-24 | 1976-06-01 | The United States Of America As Represented By The Secretary Of The Navy | Liquid vesicant differentiating paint |
| US4083692A (en) * | 1970-06-04 | 1978-04-11 | The United States Of America As Represented By The Secretary Of The Army | Detection and estimation of microquantities of alkylating agents |
| JPS5621320A (en) * | 1979-07-28 | 1981-02-27 | Fujitsu Ltd | Manufacture of semiconductor device |
| DE2947459C2 (en) * | 1979-11-24 | 1983-06-23 | Battelle-Institut E.V., 6000 Frankfurt | Device for in-situ detection of toxic substances suspected in floor coverings |
-
1983
- 1983-12-10 DE DE19833344700 patent/DE3344700A1/en active Granted
-
1984
- 1984-11-28 CA CA000468798A patent/CA1231634A/en not_active Expired
- 1984-12-01 AT AT85900095T patent/ATE37607T1/en not_active IP Right Cessation
- 1984-12-01 WO PCT/EP1984/000383 patent/WO1985002679A1/en active IP Right Grant
- 1984-12-01 EP EP85900095A patent/EP0164379B1/en not_active Expired
- 1984-12-01 JP JP60500190A patent/JPS61500687A/en active Pending
-
1985
- 1985-08-01 FI FI852970A patent/FI75056C/en not_active IP Right Cessation
- 1985-08-06 NO NO85853093A patent/NO164383C/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FI75056C (en) | 1988-04-11 |
| FI852970A0 (en) | 1985-08-01 |
| NO164383C (en) | 1990-09-26 |
| EP0164379B1 (en) | 1988-09-28 |
| DE3344700A1 (en) | 1985-06-20 |
| FI852970L (en) | 1985-08-01 |
| FI75056B (en) | 1987-12-31 |
| WO1985002679A1 (en) | 1985-06-20 |
| ATE37607T1 (en) | 1988-10-15 |
| JPS61500687A (en) | 1986-04-10 |
| NO853093L (en) | 1985-08-06 |
| DE3344700C2 (en) | 1990-06-21 |
| EP0164379A1 (en) | 1985-12-18 |
| NO164383B (en) | 1990-06-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |