CA1201990A - Modified proteins and process for the production thereof - Google Patents
Modified proteins and process for the production thereofInfo
- Publication number
- CA1201990A CA1201990A CA000432218A CA432218A CA1201990A CA 1201990 A CA1201990 A CA 1201990A CA 000432218 A CA000432218 A CA 000432218A CA 432218 A CA432218 A CA 432218A CA 1201990 A CA1201990 A CA 1201990A
- Authority
- CA
- Canada
- Prior art keywords
- protein
- enzyme
- denatured
- acid
- grossly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 67
- 230000008569 process Effects 0.000 title claims abstract description 42
- 102000035118 modified proteins Human genes 0.000 title claims abstract description 31
- 108091005573 modified proteins Proteins 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 178
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 178
- 102000004190 Enzymes Human genes 0.000 claims abstract description 78
- 108090000790 Enzymes Proteins 0.000 claims abstract description 78
- 239000003112 inhibitor Substances 0.000 claims abstract description 27
- 229940121649 protein inhibitor Drugs 0.000 claims abstract description 8
- 239000012268 protein inhibitor Substances 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 23
- 239000000758 substrate Substances 0.000 claims description 21
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- 239000004202 carbamide Substances 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000004132 cross linking Methods 0.000 claims description 5
- 239000003431 cross linking reagent Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 5
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 4
- 229940125532 enzyme inhibitor Drugs 0.000 claims description 3
- 239000002532 enzyme inhibitor Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims 4
- 150000007522 mineralic acids Chemical group 0.000 claims 4
- 150000007524 organic acids Chemical group 0.000 claims 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims 2
- 235000011054 acetic acid Nutrition 0.000 claims 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 2
- 235000015165 citric acid Nutrition 0.000 claims 2
- 235000019253 formic acid Nutrition 0.000 claims 2
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical group [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 2
- 229910017604 nitric acid Inorganic materials 0.000 claims 2
- 235000019260 propionic acid Nutrition 0.000 claims 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims 2
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- APJYDQYYACXCRM-UHFFFAOYSA-N tryptamine Chemical compound C1=CC=C2C(CCN)=CNC2=C1 APJYDQYYACXCRM-UHFFFAOYSA-N 0.000 description 12
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- 108010083644 Ribonucleases Proteins 0.000 description 9
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- 238000004925 denaturation Methods 0.000 description 9
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
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- 241000283690 Bos taurus Species 0.000 description 3
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical group CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 3
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 3
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- 125000000539 amino acid group Chemical group 0.000 description 3
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- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 3
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
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- WFIYPADYPQQLNN-UHFFFAOYSA-N 2-[2-(4-bromopyrazol-1-yl)ethyl]isoindole-1,3-dione Chemical compound C1=C(Br)C=NN1CCN1C(=O)C2=CC=CC=C2C1=O WFIYPADYPQQLNN-UHFFFAOYSA-N 0.000 description 1
- NJXWZWXCHBNOOG-UHFFFAOYSA-N 3,3-diphenylpropyl(1-phenylethyl)azanium;chloride Chemical compound [Cl-].C=1C=CC=CC=1C(C)[NH2+]CCC(C=1C=CC=CC=1)C1=CC=CC=C1 NJXWZWXCHBNOOG-UHFFFAOYSA-N 0.000 description 1
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- 108090000317 Chymotrypsin Proteins 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41834482A | 1982-09-15 | 1982-09-15 | |
| US418,344 | 1982-09-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1201990A true CA1201990A (en) | 1986-03-18 |
Family
ID=23657724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000432218A Expired CA1201990A (en) | 1982-09-15 | 1983-07-11 | Modified proteins and process for the production thereof |
Country Status (8)
| Country | Link |
|---|---|
| JP (1) | JPS5959188A (enrdf_load_stackoverflow) |
| AU (1) | AU538170B2 (enrdf_load_stackoverflow) |
| BR (1) | BR8304970A (enrdf_load_stackoverflow) |
| CA (1) | CA1201990A (enrdf_load_stackoverflow) |
| DE (1) | DE3329624A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2532949B1 (enrdf_load_stackoverflow) |
| GB (1) | GB2127024B (enrdf_load_stackoverflow) |
| IT (1) | IT1173739B (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4766205A (en) * | 1985-11-13 | 1988-08-23 | Beatrice Companies, Inc. | Method for isolation of recombinant polypeptides in biologically active forms |
| DE3611817A1 (de) * | 1986-04-08 | 1987-10-15 | Boehringer Mannheim Gmbh | Verfahren zur renaturierung von proteinen |
| US5179199A (en) * | 1986-10-20 | 1993-01-12 | Genzyme Corporation | Protein purification |
| US4912200A (en) * | 1987-05-11 | 1990-03-27 | Schering Corporation | Extraction of granulocyte macrophage colony stimulating factor from bacteria |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3666627A (en) * | 1968-10-14 | 1972-05-30 | Corning Glass Works | Method of stabilizing enzymes |
| JPS5325030B2 (enrdf_load_stackoverflow) * | 1972-12-19 | 1978-07-24 | ||
| US3930950A (en) * | 1974-04-09 | 1976-01-06 | The Ohio State University Research Foundation | Method of immobilizing an enzyme |
| US4716116A (en) * | 1980-12-04 | 1987-12-29 | Owens-Illinois Glass Container Inc. | Protein modification to provide enzyme activity |
-
1983
- 1983-05-11 AU AU14445/83A patent/AU538170B2/en not_active Ceased
- 1983-05-27 GB GB08314851A patent/GB2127024B/en not_active Expired
- 1983-07-11 CA CA000432218A patent/CA1201990A/en not_active Expired
- 1983-07-19 IT IT48712/83A patent/IT1173739B/it active
- 1983-08-17 DE DE19833329624 patent/DE3329624A1/de active Granted
- 1983-08-24 JP JP58153373A patent/JPS5959188A/ja active Pending
- 1983-09-14 FR FR8314648A patent/FR2532949B1/fr not_active Expired
- 1983-09-14 BR BR8304970A patent/BR8304970A/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR8304970A (pt) | 1984-04-24 |
| AU1444583A (en) | 1984-05-17 |
| JPS5959188A (ja) | 1984-04-04 |
| FR2532949B1 (fr) | 1987-01-30 |
| DE3329624A1 (de) | 1984-03-15 |
| GB2127024B (en) | 1986-04-23 |
| AU538170B2 (en) | 1984-08-02 |
| GB2127024A (en) | 1984-04-04 |
| DE3329624C2 (enrdf_load_stackoverflow) | 1987-09-03 |
| IT8348712A0 (it) | 1983-07-19 |
| IT1173739B (it) | 1987-06-24 |
| GB8314851D0 (en) | 1983-07-06 |
| FR2532949A1 (fr) | 1984-03-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |