CA1183080A - Utilisation de ligands synthetiques bifonctionnels pour le dosage immunologique de deux solutes - Google Patents
Utilisation de ligands synthetiques bifonctionnels pour le dosage immunologique de deux solutesInfo
- Publication number
- CA1183080A CA1183080A CA000398267A CA398267A CA1183080A CA 1183080 A CA1183080 A CA 1183080A CA 000398267 A CA000398267 A CA 000398267A CA 398267 A CA398267 A CA 398267A CA 1183080 A CA1183080 A CA 1183080A
- Authority
- CA
- Canada
- Prior art keywords
- solute
- solid phase
- bound
- antigenic
- component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003446 ligand Substances 0.000 title claims abstract description 12
- 230000001588 bifunctional effect Effects 0.000 title abstract 4
- 239000007790 solid phase Substances 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 239000002207 metabolite Substances 0.000 claims abstract description 5
- 230000027758 ovulation cycle Effects 0.000 claims abstract description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract 2
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 18
- DNXHEGUUPJUMQT-CBZIJGRNSA-N Estrone Chemical group OC1=CC=C2[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1 DNXHEGUUPJUMQT-CBZIJGRNSA-N 0.000 claims description 14
- 238000010494 dissociation reaction Methods 0.000 claims description 12
- 230000005593 dissociations Effects 0.000 claims description 12
- 150000003431 steroids Chemical class 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000000890 antigenic effect Effects 0.000 claims 28
- 239000000306 component Substances 0.000 claims 19
- 239000007853 buffer solution Substances 0.000 claims 8
- 238000005406 washing Methods 0.000 claims 8
- 230000015572 biosynthetic process Effects 0.000 claims 4
- 230000002860 competitive effect Effects 0.000 claims 4
- 229940088598 enzyme Drugs 0.000 claims 4
- 108060003951 Immunoglobulin Proteins 0.000 claims 2
- 239000000427 antigen Substances 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 102000018358 immunoglobulin Human genes 0.000 claims 2
- 230000002441 reversible effect Effects 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims 1
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 claims 1
- ZFFFJLDTCLJDHL-JQYCEVDMSA-N Pregnanediol-3-glucuronide Chemical compound O([C@H]1C[C@H]2CC[C@H]3[C@@H]4CC[C@@H]([C@]4(CC[C@@H]3[C@@]2(C)CC1)C)[C@@H](O)C)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O ZFFFJLDTCLJDHL-JQYCEVDMSA-N 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 210000002700 urine Anatomy 0.000 abstract description 9
- 102220096710 rs876658288 Human genes 0.000 abstract description 6
- 230000000984 immunochemical effect Effects 0.000 abstract description 2
- 230000003054 hormonal effect Effects 0.000 abstract 1
- 238000005065 mining Methods 0.000 abstract 1
- DNXHEGUUPJUMQT-UHFFFAOYSA-N (+)-estrone Natural products OC1=CC=C2C3CCC(C)(C(CC4)=O)C4C3CCC2=C1 DNXHEGUUPJUMQT-UHFFFAOYSA-N 0.000 description 9
- 229960003399 estrone Drugs 0.000 description 9
- 239000012071 phase Substances 0.000 description 7
- 229930182480 glucuronide Natural products 0.000 description 6
- -1 oestrone glucuronide Chemical class 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000000262 estrogen Substances 0.000 description 2
- 150000008134 glucuronides Chemical class 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000011599 ovarian follicle development Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 1
- UYUXSRADSPPKRZ-SKNVOMKLSA-N D-glucurono-6,3-lactone Chemical compound O=C[C@H](O)[C@H]1OC(=O)[C@@H](O)[C@H]1O UYUXSRADSPPKRZ-SKNVOMKLSA-N 0.000 description 1
- 235000019227 E-number Nutrition 0.000 description 1
- 239000004243 E-number Substances 0.000 description 1
- 101100496087 Mus musculus Clec12a gene Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- YWYQTGBBEZQBGO-BERLURQNSA-N Pregnanediol Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](O)C)[C@@]2(C)CC1 YWYQTGBBEZQBGO-BERLURQNSA-N 0.000 description 1
- YWYQTGBBEZQBGO-UHFFFAOYSA-N UC1011 Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(O)C)C1(C)CC2 YWYQTGBBEZQBGO-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000029849 luteinization Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000624 ovulatory effect Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000398267A CA1183080A (fr) | 1982-03-12 | 1982-03-12 | Utilisation de ligands synthetiques bifonctionnels pour le dosage immunologique de deux solutes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000398267A CA1183080A (fr) | 1982-03-12 | 1982-03-12 | Utilisation de ligands synthetiques bifonctionnels pour le dosage immunologique de deux solutes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1183080A true CA1183080A (fr) | 1985-02-26 |
Family
ID=4122286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000398267A Expired CA1183080A (fr) | 1982-03-12 | 1982-03-12 | Utilisation de ligands synthetiques bifonctionnels pour le dosage immunologique de deux solutes |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1183080A (fr) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0225054A1 (fr) * | 1985-10-30 | 1987-06-10 | Celltech Limited | Dispositif de dosage à liaison |
| EP0327843A1 (fr) * | 1988-02-08 | 1989-08-16 | Hygeia Sciences, Inc. | Essai immunologique à seuil défini |
| US5500350A (en) * | 1985-10-30 | 1996-03-19 | Celltech Limited | Binding assay device |
| US6234974B1 (en) * | 1992-08-21 | 2001-05-22 | Unilever Patent Holdings B.V. | Monitoring method |
| US6451619B1 (en) | 1994-06-29 | 2002-09-17 | Inverness Medical Switzerland Gmbh | Monitoring methods and devices for use therein |
| US6454726B1 (en) | 1992-08-21 | 2002-09-24 | Inverness Medical Switzerland Gmbh | Monitoring method |
| US6585663B1 (en) | 1992-08-21 | 2003-07-01 | Inverness Medical Switzerland Gmbh | Advisory method |
| US6951631B1 (en) | 1996-09-27 | 2005-10-04 | Inverness Medical Switzerland Gmbh | Test kits and devices |
| US7141212B2 (en) | 1993-11-12 | 2006-11-28 | Inverness Medical Switzerland Gmbh | Reading devices and assay devices for use therewith |
-
1982
- 1982-03-12 CA CA000398267A patent/CA1183080A/fr not_active Expired
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5604110A (en) * | 1985-10-30 | 1997-02-18 | Celltech Therapeutics Ltd. | Binding assay device |
| US5500350A (en) * | 1985-10-30 | 1996-03-19 | Celltech Limited | Binding assay device |
| EP0225054A1 (fr) * | 1985-10-30 | 1987-06-10 | Celltech Limited | Dispositif de dosage à liaison |
| GB2191578B (en) * | 1985-10-30 | 1989-11-01 | Boots Celltech Diagnostics | Binding assay device |
| GB2191578A (en) * | 1985-10-30 | 1987-12-16 | Boots Celltech Diagnostics | Binding assay device |
| US4952517A (en) * | 1988-02-08 | 1990-08-28 | Hygeia Sciences, Inc. | Positive step immunoassay |
| EP0327843A1 (fr) * | 1988-02-08 | 1989-08-16 | Hygeia Sciences, Inc. | Essai immunologique à seuil défini |
| US6234974B1 (en) * | 1992-08-21 | 2001-05-22 | Unilever Patent Holdings B.V. | Monitoring method |
| US6585663B1 (en) | 1992-08-21 | 2003-07-01 | Inverness Medical Switzerland Gmbh | Advisory method |
| US6454726B1 (en) | 1992-08-21 | 2002-09-24 | Inverness Medical Switzerland Gmbh | Monitoring method |
| US7141212B2 (en) | 1993-11-12 | 2006-11-28 | Inverness Medical Switzerland Gmbh | Reading devices and assay devices for use therewith |
| US6451619B1 (en) | 1994-06-29 | 2002-09-17 | Inverness Medical Switzerland Gmbh | Monitoring methods and devices for use therein |
| US6951631B1 (en) | 1996-09-27 | 2005-10-04 | Inverness Medical Switzerland Gmbh | Test kits and devices |
| US7632460B2 (en) | 1996-09-27 | 2009-12-15 | Inverness Medical Switzerland Gmbh | Test kits and devices |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEC | Expiry (correction) | ||
| MKEX | Expiry |