CA1172586A - Method of culturing anchorage dependent cells - Google Patents
Method of culturing anchorage dependent cellsInfo
- Publication number
- CA1172586A CA1172586A CA000398207A CA398207A CA1172586A CA 1172586 A CA1172586 A CA 1172586A CA 000398207 A CA000398207 A CA 000398207A CA 398207 A CA398207 A CA 398207A CA 1172586 A CA1172586 A CA 1172586A
- Authority
- CA
- Canada
- Prior art keywords
- cells
- membranes
- molecular weight
- anchorage
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- 238000012258 culturing Methods 0.000 title claims abstract description 8
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- 239000012528 membrane Substances 0.000 claims abstract description 63
- 239000000758 substrate Substances 0.000 claims abstract description 20
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 4
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
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- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical group C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
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- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 229940035718 sular Drugs 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 210000002237 B-cell of pancreatic islet Anatomy 0.000 description 1
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- 108010017384 Blood Proteins Proteins 0.000 description 1
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229920002907 Guar gum Polymers 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- MKWKNSIESPFAQN-UHFFFAOYSA-N N-cyclohexyl-2-aminoethanesulfonic acid Chemical compound OS(=O)(=O)CCNC1CCCCC1 MKWKNSIESPFAQN-UHFFFAOYSA-N 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 229940123573 Protein synthesis inhibitor Drugs 0.000 description 1
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- 239000004809 Teflon Substances 0.000 description 1
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- 229920001615 Tragacanth Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
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- 239000000783 alginic acid Substances 0.000 description 1
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- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229920006318 anionic polymer Polymers 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 239000000305 astragalus gummifer gum Substances 0.000 description 1
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- 239000000872 buffer Substances 0.000 description 1
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- 150000001720 carbohydrates Chemical group 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229920006317 cationic polymer Polymers 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- PBAYDYUZOSNJGU-UHFFFAOYSA-N chelidonic acid Natural products OC(=O)C1=CC(=O)C=C(C(O)=O)O1 PBAYDYUZOSNJGU-UHFFFAOYSA-N 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
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- 210000002808 connective tissue Anatomy 0.000 description 1
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- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000000665 guar gum Substances 0.000 description 1
- 235000010417 guar gum Nutrition 0.000 description 1
- 229960002154 guar gum Drugs 0.000 description 1
- 229920006158 high molecular weight polymer Polymers 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000000879 imine group Chemical group 0.000 description 1
- 150000002466 imines Chemical class 0.000 description 1
- 125000001841 imino group Chemical group [H]N=* 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
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- 230000003993 interaction Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 210000001985 kidney epithelial cell Anatomy 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 108010011110 polyarginine Proteins 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 229920002714 polyornithine Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 210000002325 somatostatin-secreting cell Anatomy 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 159000000008 strontium salts Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
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- 239000003440 toxic substance Substances 0.000 description 1
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- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0012—Cell encapsulation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/74—Alginate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24358681A | 1981-03-13 | 1981-03-13 | |
| US243,586 | 1981-03-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1172586A true CA1172586A (en) | 1984-08-14 |
Family
ID=22919338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000398207A Expired CA1172586A (en) | 1981-03-13 | 1982-03-12 | Method of culturing anchorage dependent cells |
Country Status (11)
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO163060C (no) * | 1981-03-13 | 1990-03-28 | Damon Biotech Inc | Fremgangsmaate for fremstilling av substanser som dannes av levende celler som produserer interferoner eller antistoffer. |
| DK37083A (da) * | 1982-02-01 | 1983-08-02 | Cancer Res Nat Found | Graensefladedyrkning af celler i vaevskultur |
| US4537860A (en) * | 1982-12-08 | 1985-08-27 | Monsanto Company | Static cell culture maintenance system |
| DE3376660D1 (en) * | 1983-06-22 | 1988-06-23 | Stolle Res & Dev | Encapsulated cells, their method of preparation and use |
| JPS6025929A (ja) * | 1983-07-20 | 1985-02-08 | ザ・スト−ル・リサ−チ・アンド・デイベロツプメント・コ−ポレ−シヨン | カプセル化細胞、その製造方法および用途 |
| EP0258441A4 (en) * | 1986-01-25 | 1988-06-27 | Nitta Gelatin Kk | LARGE SCALE ANIMAL CELL CULTURE PROCESS. |
| IL79052A0 (en) * | 1986-06-06 | 1986-11-30 | Univ Ramot | Device and process for production of alginate-shell beads containing biologically active material |
| JPS63105674A (ja) * | 1986-10-21 | 1988-05-10 | Kyowa Hakko Kogyo Co Ltd | 動物細胞の増殖方法 |
| US5182111A (en) * | 1987-11-17 | 1993-01-26 | Boston University Research Foundation | In vivo delivery of active factors by co-cultured cell implants |
| EP0364606B1 (en) * | 1988-04-18 | 1994-03-16 | Nitta Gelatin Inc. | Process for culturing animal cells on a large scale and process for preparing supporting substrate for that process |
| US5719051A (en) * | 1989-12-22 | 1998-02-17 | Immuno Aktiengesellschaft | Perfusion system and a method for the large scale production of virus or virus antigen |
| AT393356B (de) * | 1989-12-22 | 1991-10-10 | Immuno Ag | Verfahren zur herstellung von fsme-virus-antigen |
| SG47470A1 (en) * | 1991-04-25 | 1998-04-17 | Univ Brown Res Found | Implantable biocompatible immunoisolatory vehicle for delivery of a selected therapeutic products |
| US5800829A (en) * | 1991-04-25 | 1998-09-01 | Brown University Research Foundation | Methods for coextruding immunoisolatory implantable vehicles with a biocompatible jacket and a biocompatible matrix core |
| US5232712A (en) * | 1991-06-28 | 1993-08-03 | Brown University Research Foundation | Extrusion apparatus and systems |
| JPH05176753A (ja) * | 1991-12-26 | 1993-07-20 | Nec Corp | 細胞培養用基板とその作製方法 |
| US5492826A (en) * | 1993-12-10 | 1996-02-20 | William Beaumont Hospital | Apparatus and method for seeding endothelial cells |
| DE4426396A1 (de) * | 1994-07-26 | 1996-02-01 | Ulrich Prof Dr Zimmermann | Verfahren zur Herstellung konzentrierter Lösungen von mikroverkapselten Zellen oder von suspendierten Wirkstoffen in mikroverkapselter Form |
| US6297046B1 (en) | 1994-10-28 | 2001-10-02 | Baxter International Inc. | Multilayer gas-permeable container for the culture of adherent and non-adherent cells |
| US5935847A (en) | 1994-10-28 | 1999-08-10 | Baxter International Inc. | Multilayer gas-permeable container for the culture of adherent and non-adherent cells |
| US6024220A (en) | 1995-06-07 | 2000-02-15 | Baxter International Inc. | Encapsulated seam for multilayer materials |
| US6391404B1 (en) | 1995-06-07 | 2002-05-21 | Baxter International Inc. | Coextruded multilayer film materials and containers made therefrom |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4352883A (en) * | 1979-03-28 | 1982-10-05 | Damon Corporation | Encapsulation of biological material |
| NO163060C (no) * | 1981-03-13 | 1990-03-28 | Damon Biotech Inc | Fremgangsmaate for fremstilling av substanser som dannes av levende celler som produserer interferoner eller antistoffer. |
| NO158284C (no) * | 1981-03-13 | 1988-08-17 | Damon Biotech Inc | Fremgangsmaate for selektiv oppbrytning av en permeabel membran. |
-
1982
- 1982-03-11 NO NO820796A patent/NO161446C/no unknown
- 1982-03-12 CA CA000398207A patent/CA1172586A/en not_active Expired
- 1982-03-12 DK DK112482A patent/DK112482A/da not_active Application Discontinuation
- 1982-03-12 BE BE0/207558A patent/BE892478A/fr not_active IP Right Cessation
- 1982-03-12 JP JP57038240A patent/JPS6038111B2/ja not_active Expired
- 1982-03-12 DE DE19823209098 patent/DE3209098A1/de active Granted
- 1982-03-12 SE SE8201555A patent/SE452335B/sv not_active IP Right Cessation
- 1982-03-12 FR FR8204244A patent/FR2501715B1/fr not_active Expired
- 1982-03-12 IT IT20138/82A patent/IT1150680B/it active
- 1982-03-12 GB GB8207306A patent/GB2094832B/en not_active Expired
- 1982-03-12 CH CH1573/82A patent/CH662363A5/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| SE8201555L (sv) | 1982-09-14 |
| NO161446B (no) | 1989-05-08 |
| IT1150680B (it) | 1986-12-17 |
| GB2094832B (en) | 1984-06-20 |
| DE3209098A1 (de) | 1982-11-04 |
| NO820796L (no) | 1982-09-14 |
| FR2501715A1 (fr) | 1982-09-17 |
| JPS57202289A (en) | 1982-12-11 |
| JPS6038111B2 (ja) | 1985-08-30 |
| DE3209098C2 (enrdf_load_stackoverflow) | 1987-08-13 |
| FR2501715B1 (fr) | 1985-07-12 |
| DK112482A (da) | 1982-09-14 |
| NO161446C (no) | 1989-08-16 |
| CH662363A5 (de) | 1987-09-30 |
| BE892478A (fr) | 1982-07-01 |
| GB2094832A (en) | 1982-09-22 |
| SE452335B (sv) | 1987-11-23 |
| IT8220138A0 (it) | 1982-03-12 |
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