CA1163936A - Mycoplasma-free hepatoma cell line - Google Patents
Mycoplasma-free hepatoma cell lineInfo
- Publication number
- CA1163936A CA1163936A CA000385519A CA385519A CA1163936A CA 1163936 A CA1163936 A CA 1163936A CA 000385519 A CA000385519 A CA 000385519A CA 385519 A CA385519 A CA 385519A CA 1163936 A CA1163936 A CA 1163936A
- Authority
- CA
- Canada
- Prior art keywords
- cell line
- mycoplasma
- hepatoma cell
- cells
- immuno
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 206010073071 hepatocellular carcinoma Diseases 0.000 title claims abstract description 25
- 241000204031 Mycoplasma Species 0.000 claims abstract description 19
- 241000699670 Mus sp. Species 0.000 claims abstract description 12
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims description 14
- 241000894007 species Species 0.000 claims description 11
- 238000004113 cell culture Methods 0.000 claims description 7
- 241000699800 Cricetinae Species 0.000 claims description 4
- 206010062016 Immunosuppression Diseases 0.000 claims description 4
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 4
- 230000001506 immunosuppresive effect Effects 0.000 claims description 4
- 230000002950 deficient Effects 0.000 claims description 3
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 claims description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 2
- 241000700159 Rattus Species 0.000 claims description 2
- 229960004397 cyclophosphamide Drugs 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims description 2
- 241000700199 Cavia porcellus Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 32
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000012531 culture fluid Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 229930193140 Neomycin Natural products 0.000 description 3
- 229960004927 neomycin Drugs 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 230000003187 abdominal effect Effects 0.000 description 2
- 230000001494 anti-thymocyte effect Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 241000700198 Cavia Species 0.000 description 1
- GUTLYIVDDKVIGB-OUBTZVSYSA-N Cobalt-60 Chemical compound [60Co] GUTLYIVDDKVIGB-OUBTZVSYSA-N 0.000 description 1
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- 241000204003 Mycoplasmatales Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 208000002352 blister Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 150000001993 dienes Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 239000007758 minimum essential medium Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Oncology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18505480A | 1980-09-11 | 1980-09-11 | |
| US185,054 | 1988-04-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1163936A true CA1163936A (en) | 1984-03-20 |
Family
ID=22679376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000385519A Expired CA1163936A (en) | 1980-09-11 | 1981-09-09 | Mycoplasma-free hepatoma cell line |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0048202A3 (OSRAM) |
| JP (1) | JPS5780324A (OSRAM) |
| CA (1) | CA1163936A (OSRAM) |
| DK (1) | DK401781A (OSRAM) |
| ES (1) | ES505344A0 (OSRAM) |
| GR (1) | GR75078B (OSRAM) |
| PT (1) | PT73614B (OSRAM) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4393133A (en) * | 1980-06-12 | 1983-07-12 | The Wistar Institute Of Anatomy And Biology | Human hepatoma derived cell line, process for preparation thereof, and uses therefor |
| US4391754A (en) * | 1980-07-29 | 1983-07-05 | Imperial Chemical Industries Plc | Anthraquinone compounds |
| JPS60127103A (ja) * | 1983-12-14 | 1985-07-06 | リョービ株式会社 | 電動チエンソ−のブレ−キロツク装置 |
| AU2263288A (en) * | 1987-07-16 | 1989-02-13 | Codon | Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same |
| CN108467854B (zh) * | 2017-02-23 | 2021-08-31 | 中国科学院上海营养与健康研究所 | 新的骨特异性转移肝癌细胞及其制备 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3935066A (en) * | 1969-07-03 | 1976-01-27 | Burroughs Wellcome Co. | Cell lines |
| US4209587A (en) * | 1978-12-29 | 1980-06-24 | Monsanto Company | Production of tumor angiogenesis factor by cell culture |
| IE51173B1 (en) * | 1980-03-27 | 1986-10-29 | Merck & Co Inc | Method of growing hepatitis b surface antigen |
-
1981
- 1981-09-02 PT PT7361481A patent/PT73614B/pt unknown
- 1981-09-08 GR GR65988A patent/GR75078B/el unknown
- 1981-09-09 ES ES505344A patent/ES505344A0/es active Granted
- 1981-09-09 CA CA000385519A patent/CA1163936A/en not_active Expired
- 1981-09-10 DK DK401781A patent/DK401781A/da not_active Application Discontinuation
- 1981-09-10 EP EP81401408A patent/EP0048202A3/en not_active Withdrawn
- 1981-09-11 JP JP14372581A patent/JPS5780324A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DK401781A (da) | 1982-03-12 |
| EP0048202A2 (en) | 1982-03-24 |
| EP0048202A3 (en) | 1983-06-01 |
| GR75078B (OSRAM) | 1984-07-13 |
| ES8302087A1 (es) | 1983-01-01 |
| PT73614A (en) | 1981-10-01 |
| JPS5780324A (en) | 1982-05-19 |
| PT73614B (en) | 1983-10-19 |
| ES505344A0 (es) | 1983-01-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |