CA1161734A - Stabilized romanowsky stain solution - Google Patents
Stabilized romanowsky stain solutionInfo
- Publication number
- CA1161734A CA1161734A CA000398756A CA398756A CA1161734A CA 1161734 A CA1161734 A CA 1161734A CA 000398756 A CA000398756 A CA 000398756A CA 398756 A CA398756 A CA 398756A CA 1161734 A CA1161734 A CA 1161734A
- Authority
- CA
- Canada
- Prior art keywords
- stain
- solution
- combination
- hydrochloride
- azures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 36
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229960000907 methylthioninium chloride Drugs 0.000 claims abstract description 17
- XHFGWHUWQXTGAT-UHFFFAOYSA-N dimethylamine hydrochloride Natural products CNC(C)C XHFGWHUWQXTGAT-UHFFFAOYSA-N 0.000 claims abstract description 11
- IQDGSYLLQPDQDV-UHFFFAOYSA-N dimethylazanium;chloride Chemical compound Cl.CNC IQDGSYLLQPDQDV-UHFFFAOYSA-N 0.000 claims abstract description 11
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims abstract description 8
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims abstract description 4
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 claims abstract description 4
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 4
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims abstract description 3
- 239000003381 stabilizer Substances 0.000 claims description 15
- 239000000975 dye Substances 0.000 claims description 14
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 150000003973 alkyl amines Chemical group 0.000 claims description 4
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 2
- CBPYOHALYYGNOE-UHFFFAOYSA-M potassium;3,5-dinitrobenzoate Chemical group [K+].[O-]C(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1 CBPYOHALYYGNOE-UHFFFAOYSA-M 0.000 claims 3
- 150000001412 amines Chemical class 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 54
- 238000002835 absorbance Methods 0.000 description 10
- 230000015556 catabolic process Effects 0.000 description 9
- 238000006731 degradation reaction Methods 0.000 description 9
- 238000010186 staining Methods 0.000 description 8
- 239000000654 additive Substances 0.000 description 7
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 230000032683 aging Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- JXBCJUPBVRELNY-UHFFFAOYSA-N [7-(diethylamino)phenothiazin-3-ylidene]-diethylazanium Chemical compound C1=CC(N(CC)CC)=CC2=[S+]C3=CC(N(CC)CC)=CC=C3N=C21 JXBCJUPBVRELNY-UHFFFAOYSA-N 0.000 description 1
- -1 alkylamine hydro-chlorides Chemical group 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- GAZIBGHLWYHBDT-UHFFFAOYSA-N n-propylpropan-1-amine;hydrochloride Chemical compound Cl.CCCNCCC GAZIBGHLWYHBDT-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- YBRBMKDOPFTVDT-UHFFFAOYSA-N tert-butylamine Chemical compound CC(C)(C)N YBRBMKDOPFTVDT-UHFFFAOYSA-N 0.000 description 1
- FVRDYQYEVDDKCR-DBRKOABJSA-N tiazofurine Chemical compound NC(=O)C1=CSC([C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)=N1 FVRDYQYEVDDKCR-DBRKOABJSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Silver Salt Photography Or Processing Solution Therefor (AREA)
- Detergent Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US250,732 | 1981-04-03 | ||
| US06/250,732 US4382075A (en) | 1981-04-03 | 1981-04-03 | Stabilized Romanowsky stain solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1161734A true CA1161734A (en) | 1984-02-07 |
Family
ID=22948920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000398756A Expired CA1161734A (en) | 1981-04-03 | 1982-03-18 | Stabilized romanowsky stain solution |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4382075A (enExample) |
| EP (1) | EP0063293B1 (enExample) |
| JP (1) | JPS57171263A (enExample) |
| AU (1) | AU530146B2 (enExample) |
| CA (1) | CA1161734A (enExample) |
| DE (1) | DE3261913D1 (enExample) |
| ES (1) | ES8307017A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU184903B (en) * | 1981-11-20 | 1984-11-28 | Reanal Finomvegyszergyar | Paint composition for biopsy examinations |
| US4392864A (en) * | 1982-02-01 | 1983-07-12 | Miles Laboratories, Inc. | Stabilized Romanowsky stain solution |
| US4595524A (en) * | 1984-03-28 | 1986-06-17 | Miles Laboratories, Inc. | Two component stain composition for producing a Giemsa blood stain effect |
| US4741898A (en) * | 1985-04-01 | 1988-05-03 | Fisher Scientific Company | Stabilized stain composition |
| DE3533515A1 (de) * | 1985-09-20 | 1987-04-02 | Merck Patent Gmbh | Stabilisator fuer faerbeloesungen |
| US6858432B2 (en) * | 2002-08-29 | 2005-02-22 | Wescor, Inc. | Method and staining reagent for staining hematology sample in an automated staining apparatus |
| FR2854239B1 (fr) * | 2003-04-23 | 2006-03-10 | Reactifs Ral | Procede de preparation d'un reactif de coloration de cellules et reactif obtenu |
| EP2721391B1 (en) | 2011-06-17 | 2022-04-06 | Roche Diagnostics Hematology, Inc. | Solution and method for histoprocessing of biological samples |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4290769A (en) * | 1980-10-14 | 1981-09-22 | Miles Laboratories, Inc. | Stabilized Romanowsky stain solution |
-
1981
- 1981-04-03 US US06/250,732 patent/US4382075A/en not_active Expired - Lifetime
-
1982
- 1982-03-18 CA CA000398756A patent/CA1161734A/en not_active Expired
- 1982-03-25 JP JP57046487A patent/JPS57171263A/ja active Granted
- 1982-04-02 AU AU82292/82A patent/AU530146B2/en not_active Ceased
- 1982-04-02 ES ES511093A patent/ES8307017A1/es not_active Expired
- 1982-04-03 DE DE8282102858T patent/DE3261913D1/de not_active Expired
- 1982-04-03 EP EP82102858A patent/EP0063293B1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS57171263A (en) | 1982-10-21 |
| EP0063293A1 (en) | 1982-10-27 |
| US4382075A (en) | 1983-05-03 |
| EP0063293B1 (en) | 1985-01-16 |
| JPH0129264B2 (enExample) | 1989-06-08 |
| ES511093A0 (es) | 1983-06-16 |
| AU530146B2 (en) | 1983-07-07 |
| AU8229282A (en) | 1982-11-04 |
| DE3261913D1 (en) | 1985-02-28 |
| ES8307017A1 (es) | 1983-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry | ||
| MKEX | Expiry |
Effective date: 20010207 |