CA1121814A - Antibiotic c-15003 - Google Patents

Abstract

Abstract of the Disclosure A novel Antibiotic C-15003 producible by cultivating a microorganism of the genus Nocardia, and having antifungal and antiprotozol properties, is converted by reductive hydrolysis employing, e.g. lithium aluminum hydride, to the corresponding free alcohol, identical with the known antibiotic maytansinol.

Description

This invention relates to a derivative of the novel Antibiotic C-15003, and a method of producing the same.
We collected many soil and other samples and performed a screening of the microorganisms isolated from such samples. It was found, by that screen-ing, that certain of the microorganisms were able to produce a novel antibiotic, that such microorganisms belonged to the genus Nocardia and that by cultivating any of those microorganisms in a suitable medium, it was possible to have said antibiotic accumulated in the cultured broth. It was also found that derivatives could be obtained from said antibiotic. Further studies ensued, resulting in the development of this invention.
~1~ Antibiotic C-15003 which has the general formula R

CH30 ~ N - C ~ CHo3 ~I) HO H

~herein R represents -CO-CH < , -CO-CH2-CH2-CH3 or -CO-CH2-CH~ 3 );

~ 2) Antibiotic C-15003 may be prepared by cultivating an Antibiotic C-15003-producing strain of the genus Nocardia in a medium to cause the strain to elaborate and accumulate Antibiotic C-15003 in the cultured broth, and recovering said Antibiotic C-15003 from said broth;
The present invention provides a process for producing a compound of the formula ~II):

llZ181~

CH O
C,Q ~ 3 " pH O
3 ~D-- N - C "~ CH3 (II) --~H

characterized in that said method comprises subjecting Antibiotic C-15003 of the general formula (I): R
Q , 3 " O O
CH30 ~ N ~ C ,,L~ CH3 ~,,~I`N ~O (I) (wherein R represents -CO-CH< 3, -CO-CH2-CH2-CH3 or -CO-CH2-CH < 3 ), to reductive hydrolysis.
In the context of this invention, the term "Antibiotic C-15003"
means, generically, the three compounds having the above general formula (I) as a group, or a mixture of two or three of said compounds or, severally, any of lQ the same compounds. Referring, also, to the general formula (I), the compound in brhich R is CO cH <C 3 is referred to herein as "Antibiotic C-15003 P-3" or more briefly as "C-15003 P-3"; the compound in which R is -CO-CH2-CH2-CH3 is referredto herein as "Antibiotic C-15003 P-3"' or, more briefly, as "C-15003 P-3"'; the compound in which R is CO CH CH <CH3 is referred to herein as "Antibiotic 11'~18i4 C-15003 P-4" or, more briefly, as "C-15003 P-4"; and the compound in which R is H
~general formula (II) ) is referred to herein as "Antibiotic C-15003 P-0" or, more briefly, as "C-15003 P-0".
As an example of the Antibiotic C-15003-producing strain of micro-organism, there may be mentioned an actinomycete Strain No. C-15003 which we isolated from soil and other samples in our screening for antibiotic-producing microorganisms.
The microbiological characters of Strain No. C-15003 were investi-gated by procedures analogous to those proposed by Schirling ~ Gottlieb (Inter-national Journal of Systematic Bacteriology 16, 313-340 ~1966) ~. The results of observations at 28C over 21 days are as follo~s.
1) Morphological characters The vegetative mycelium extends well and develops into branches, both on agar and in liquid medium. Many of the hyphae measure 0.8 to 1.2~m in diameter and, in certain instances~ may divide into fragments resembling rod bacteria or branched short lengths of hyphae. The strain gives good growth on various taxonomical media, with aerial mycelium being superimposed on the veget-ative mycelium, although it frequently forms coremia like bodies ~50-200 x 200 -lQQO~m) on whîch further aerial growth takes place. Many of the aerial mycelia are 1exuous, straight or a loosely spiral like configuration being encountered on a fe~ occasions. Microscopic examination of aged cultures reveals that only in fe~ cases the conidia like cells occur in chains, while the cell suspensions obtained from the surfaces of such cultures, as microscopically examined, contain-ed many elongated ellipsoidal ~0.8-1.2 ~m x 4.8-6.8 ~m) and ellipsoidal ~0.8-1.2-x 1.0-2.0 ~m) bodies resembling arthrospores.
Electron-microscopic examinations showed that these bodies had smooth surfaces.

i4

2) The constituents of cells The strain was shake-cultured in modified ISP No. 1 medium at 28C
for 66 to 90 hours, at the end of which time the cells were collected and rinsed.
By the method of B. Becker et al. (Applied Microbiology 12, 421 (1964) ) and the method of M.P. Lechevalier.
~ Journal of Laboratory and Clinical Medicine 71, 934 (1968) ), the above whole cells were examined for diaminopimelic acid and sugar composition.
The former was found to be the meso-form, while spots~were detected which corresponded to galactose and arabinose.

3) Characteristics on taxonomical media The strain showed comparatively good growth on various media, with the vegetative mycelium being colorless to pale yellow in initial phases of cul-ture and light yellowish tan to yellowish tan in later phases. The strain pro-duces soluble pigments, yellow to yellowish tan, in various taxonomical media.
The aerial mycelium is powdery and generally gives moderate growth, being white to yello~ or light yellowish tan. The characteristics of the strain in various taxonomical media are set forth in Table 1.
Tahle 1 Cultural ~haracteristics of Strain No. C-15003 on taxonomical media ~A~ Sucrose nitrate agar:
Growth ~G): Moderate, Brite Melon Yellow ~3 ia)*
to Amber tan (3 lc)*, coremia like bodies formed Aerial mycelium ~AM): Scant, white Soluble pigment ~SP~:~None or pale yellowish tan ~B~ Glycerol nitrate agar:
G: Moderate, Lt Ivory (2 ca)*, coremia like bodies formed AM: Moderate, white SP: None (C) Glucose asparagine agar:
G: Moderate, Brite Marigold ~3 pa)* to Briite Yellow (2 pa)*.
AM: Scant, white SP: Brite Yellow ~2 pa)*
(D) Glycerol asparagine agar:
G: Moderate, Lt Ivory ~2 ca)*, coremia like bodies formed AM: Scant, white SP: None (E) Starch agar:
G: Moderate, Lt Ivory (2 ca)* to Lt Wheat ~2 ea)*, coremia like bodies formed A~: Abundant, Lt Ivory (2 ca)*
SP: None (~) Nutrient agar:
G: Moderate, Lt Ivory (2 ca~* to Colonial Yellow ~2 ga)*, coremia like bodies formed AM: Scant, white SP: None ~G) Calcium malate agar:
G: Moderate Lt Ivory (2 ca~* to Lt Wheat (2 ea)*, coremia like bodies formed.
AM: Moderate, white to Lt Ivory ~2 ca)*

llZ18i4 SP: None (H) Yeast extract-malt extract agar:
G: Moderate, Amber (3 lc)* to Brite Yellow (3 la)*, coremia like bodies formed AM: Moderate, white to Lt Ivory (2 ca)*
SP: None (I) Oatmeal agar:
G: Moderate, Lt Ivory (2 ca~* to Colonial Yellow ~2 ga)*, coremia like bodies formed AM: Scant, white to light yello~
SP: None ~J~ Peptone yeast extract iron agar:
G: Moderate, Colonial Yellow ~2 ga)*
AM: None SR: Colonial Yello~ (2 ga)*
~K~ Tyrosine agar G: Moderate, Lt Ivory (2 ca)* to Lt Melon Yellow (3 ea)*, coremia like bodies formed.
AM: Moderate, white to Lt Ivory ~2 ca~*.
SP: Camel ~3 ie)*
* The color codes according to Color Harmony Manual, 4th ~d. ~Container Corpora-tion of America, 1958~.

4) Physiological characters The physiological characters of the strain are shown in Table 2.
Temperature range for growth: 12C to 38C. The temperature range in which goodaerial growth occurs on agar ~ISP No. 2) is 20 to 35C.
Table 2 The physiological characters of Strain No. C-15003.

11'~18~4 Temperature range for growth: 12 to 38C.
Temperature range for aerial growth: 20 to 35C
Liquefaction of gelatin: Positive Hydrolysis of starch: Positive Reduction of nitrates: Positive Peptonization of milk: Positive Coagulation of milk: Negative Decomposition of ~asein: Positive Production of melanoid pigments:
Negative ~peptone yeast extract iron agar), Positive ~tyrosine agar) Decomposition of tyrosine: Positive Decomposition of xanthine: Negative Decomposition of hypoxanthine: Negative Tolerance to lysozyme: Positive Tolerance to sodium chloride: 2%

5) Utilization of various carbon sources The utilization of various carbon sources was investigated using a medium described in Pridham and Gottlieb ~Journal of Bacteriology 56, 107 2Q (1948) ) and a basal medium of the same composition plus 0.1% of yeast extract.
The resultant spectrum is shown in Table 3.
Table 3 The utilization of carbon sources by Strain No. C-15003 Source of carbon Growth Sources of carbon Growth D-Xylose + ++* Raffinose _ _*
L-Arabinose + + Melibiose + +
D-Glucose ++ ++ i-Inositol D-Galactose + + _-Sorbitol 11'~1~14 D-Fructose +++ ++ D-Mannitol ++ ++
L-Rhamnose + + Glycerol - +
D-Mannose +++ ++ Soluble starch + +
Sucrose ++ ++ Control Lactose - -*
Maltose + +
Trehalose + ++
* Basal medium with 0.1% yeast extract added Note: +++: Luxuriant growth ++: Good Growth +: Growth _: Poor growth -: NQ gro~th

6) Other characteristics The cells were harvested by the procedure previously described in 2~ and DNA was prepared by a procedure analogous to that of J. Marmur et al.
~Journal of Molecular Biology 3, 208, 1961). The G-C (Guanine-Cytosine) content of the DNA was found to be about 71 mole %.
Gram-staining of the vegetative mycelium of this strain was positive.
The above characteristics of Strain No. C-15003 were compared-with the descriptions in S.A. Waksman's "The Actinomycetes Vol. 2" ~The Williams and Wilkins Co., 1961); R.E. Buchanan and N.E. Gibbons, "Bergey's Manual of Determina-tive Bacteriology, 8th ed, 1974"; and other literatures.
Whilst this strain was thought to belong to Group III of the genus Nocardia, the failure to find any species having the characters so far describedamong the known strains led us to conclude that this strain represented a novel species of microorganism.

18~

The present Strain No. C-15003 has been deposited at Fermentation Research Institute, Agency of Industrial Science and Technology (FERM) under the receipt number of 3g92; at The institute for Fermentation, Osaka (IFO) under the accession number of IFO 13726 and at The American Type Culture Collection (ATCC), Maryland, U.S.A. under the accession number of 31281.
While Strain No. C-15003 is a novel species of the genus Nocardia as just mentioned, it is liable, as are microorganisms generally, to undergo variations and mutations, either spontaneously or under the influence of a mutagen.
For example, the many variants of the strain which are obtainable by irradiation with X-rays, gamma rays, ultraviolet light, etc., by monocell isolation, by culture on media containing various chemicals, or by any other mutagenic treatment, as ~ell as the mutants spontaneously derived from the strain, should not be con-sidered to represent any other distinct species but, rather, any of such variants and mutants capable of elaborating C-15003 P-3, P-3' and/or P-4 may be invariably utilized for the purposes of this invention. By way of example, subjecting Strain No. C-15003 to various mutagenic treatments yields mutants substantially lacking the ability to produce soluble pigments, mutants with substrate mycelia which are colorless, yellowish green, reddish tan or orange-red, mutants whose hyphae are ready to fragment into bacillary elements or branched short hyphal fragments, and mutants with abundant white aerial mycelia or substantially without aerial mycelia.
The medium employed for the cultivation of such an Antibiotic C-15003-producing strain may be whichever of a liquid and a solid medium only if it contains nutrients which the strain may utilize, although a liquid medium is preferred for high-production runs. The medium may comprise carbon and nitrogen sources which Strain No. C-15003 may assimilate and digest, inorganic matter, trace nutrients, etc. As examples of said carbon sources may be mentioned glucose, lac-tose, sucrose, maltose, dextrin, starch, glycerol, mannitol, sorbitol, etc., fats _g _ ~iZ1~314 and oils ~e.g. so~bean oil, lard oil, chicken oil, etc.) and so forth. The nitrogen sources may for example be meat extract, yeast extract, dried yeast, soybean meal, corn steep liquor, peptone, cottonseed flour, spent molasses, urea, ammonium salts ~e.g. ammomium sulfate, ammonium chloride, ammonium nitrate, ammonium acetate, etc.) and so forth. The medium may further contain salts of sodium, potassium, calcium, magnesium, etc. salts of iron, manganese, zinc, co-balt, nickel, etc., salts of phosphoric acid, boric acid, etc., and organic acid salts such as acetates and propionates. Further, the medium may contain, as added, various amino acids (e.g. glutamic acid, aspartic acid, alanine, glycine, lysine, methionine, proline, etc.), peptides (e.g. dipeptides, tripeptides, etc.), vitamins (e.g. Bl, B2, nicotinic acid, B12, C, E, etc.), nucleic acids, (e.g.
purine, pyrimidine and derivatives thereof) and so forth. For the purpose of adjusting the pH of the medium, there may be added an inorganic or organic acid, alkali, buffer or the like. Suitable amounts of oils, fats, surfactants, etc.
may also be added as antifoams.
The cultivation may be conducted by any of the stationary, shake, submerged aerobic and other cultural conditions. For high production runs, sub-merged aerobic culture is of course preferred. While the conditions of culture, of course, depends upon the condition and composition of medium, the strain, cultural method and other factors, it is normally preferred to carry out incubation at 2a to 35C ~ith an initial pH of about 7.0 or thereabouts Particularly desirable is a temperature from 23 to 30C in an intermediate stage of cultivation, ~ith an initial pH of 6.5 to 7.5. While the incubation time also is variable according to the same factors as mentioned above, it is advisable to continue the incubation until the titer of the desired antibiotic product becomes maximal. In the case of shake culture or aerobic submerged culture in liquid medium, the time required normally ranges from about 48 to 144 hours.

-10- ~

The potency of the antibiotic was assayed with Tetrahymena pyriformis W as an assay organism. Thus, the above microorganism was grown on a test medium ~20 g of Proteose-peptone ~Difco*), 1 g of yeast extract ~Difco*), 2 g of glucose, 1000 mQ of distilled water and 10 mQ of 1 M-phosphate buffer ~pH 7.0) ) at 28C for 44 to 48 hours and the potency of the antibiotic was determined by the serial dilution method with a monitering of the turbidity of growth, effect on ascites tumor cells and by a thin-layer chromatographic (briefly TLC) assay to be described hereinafter.
The novel Antibiotic C-15003 P-3, P-3' and/or P-4 is produced and accumulated in the resultant fermentation broth, both extracellularly and intrace-llularly.
These substances have also been detected by TLC. Thus, the ferment-ation broth is separated into cells and filtrate by filtration or centrifugationand the filtrate is extracted with the same volume of ethyl acetate. To the cells i5 added the same amount of 70% acetone-water as the filtrate and, after an hour's stirring at 20C, the suspension is filtered. The acetone is removed from the filtrate and the resultant aqueous filtrate is extracted with ethyl acetate. Each of the extracts is concentrated to 1/100 by volume and subjected to thin-layer chromatography on a silica gel-glass plate ~Merc~, West Germany, Kieselgel* 60 F254 0.25 mm, 20 x 20) ~solvent system : chloroform-methanol = 9:1). The potency ~as determined on the basis of the intensity of spots detected by irradiation with ultraviolet light at 2537 A.
Because C-15003 P-3, P-3' and/or P-4, which are thus produced in the fermentation broth, are lipophyl neutral substances, they can be conveniently recovered by separation and purification procedures which are normally employed for the harvest of such microbial metabolites. For example, there may be employed a procedure which utili~es the difference in solubility between the antibiotic and * Trademark -11-11;Z~8~4 impurity, means which utilizes the adsorptive affinity of various adsorbents such as activated carbon, macroporous nonionic resins, silica gel, alumina, etc., a procedure of removing the impurities by means of ion exchange resins, and so forth, as applied singly or in a suitable combination or as applied in repetition.
Since, as aforesaid, C-15003 P-3, P-3' and P-4 occur in both the filtrate and cells, the antibiotics are separated and purified by means of such an adsorbent, if one is employed, either directly or after a solvent extraction in the case of the filtrate, or after a solvent extraction in the case of microbial cells. The solvent extraction may be performed by any of the follo~ing and other lQ methods e.g.
~ 1~ solvent extraction from the culture broth prior to separation of cells and ~ 2~ solvent extraction of the cells and the filtrate obtained by filtration, centrifugation or other process.
To extract the filtrate and cells independently, the following procedure may be taken advantageously.
The solvents suitable for extraction of the filtrate are water-immiscible organic solvents such as ~atty acid esters, e.g. ethyl acetate and amyl acetate; alcohols, e.g. butanol; halogenated hydrocarbons, e.g. chloroform;
and ketones, e.g. methyl isobutyl ketone. The extraction is carried out at a pH
near neutral and, preferably, the culture fluid previously adjusted to pH 7 is extracted ~ith eth~l acetate. The extract is washed with water and concentrated under reduced pressure. Then, a nonpolar solvent such as pe~roleum ether or hexane is added to the concentrate and the crude product I containing the active compound is recovered. Because, on TLC, a number of spots are detected in addition to Antibiotic C-15003, the product I is sequentially subjected to the following purification procedures. Thus, as a routine purification procedure, llZ~ 4 adsorption chromatography is useful and, for this purpose, one of those commonadsorbents such as silica gel, alumina, macroporous nonionic adsorbent resin, etc. may be employed. For purification from the crude product I, silica gel is most useful. And development may be carried out, for example starting with petroleum ether and hexane and elution of Antibiotic C-15003 is performed by the addition of a polar solvent such as ethyl acetate, acetone ethanol or methanol.
In a typical process, using silica gel (Merck, West Germany, 0.05-0.2mm) as a carrier, column chromatography is carried out with a serial increase in the hexane to ethyl acetate ratio. The eluate is sampled and investigated by TLC
and the fractions containing C-15003 are pooled and concentrated under reduced p~ess~ure. Then petroleum ether or hexane is added to the concentrate, whereby the crude product II is obtained. Since this product still contains impurities, it is further purified as follows. For example, the product II may be purified by means of a second silica gel column using a different solvent system. The developing system for this purpose may consist in a halogenated hydrocarbon such as dichloromethane or chloroform, with the addition of a polar solvent such as an alcohal, e.g. methanol or ethanol, a ketone, e.g. acetone or methyl ethyl ketone, or the like. In this way, Antibiotic C-15003 is isolated. The order of solvent systems for the first and second silica gel columns may be reversed and, in addition, ordinary organic solvents may be used in conjunction with the above systems if necessary.
Where a macroporous adsorbent resin is used as purification means for crude product II, elution of Antibiotic C-15C03 is accomplished with a mixture of water with a lower alcohol, a lower ketone or an ester. The lower alcohol may for example be methanol, ethanol, propanol or butanol and the lower ketone may for example be acetone or methyl ethyl ketone. The ester may for example be ethyl acetate. In a typical procedure, the crude product II is dissolved in 60% meth-11'~1814 anol-water and adsorbed on a column of Diaion* HP-10 (Mitsubishi Kasei K.K.).
The column is washed with 70% methanol-water and, then, elution is carried out with 90% methanol-~ater. In this way, Antibiotic C-15003 is eluted from the column.
In either of the processes described above, the fractions containing Antibiotic C-15003 are pooled and concentrated under reduced pressure. To the dry product is added 5 to 8 volumes of ethyl acetate and the mixture is allowed to stand, whereupon crystals of Antibiotic C-15003 separate. These crystals contain C-15003 P-3, P-3' and P-4. These compounds are then separated from each other by means of an adsorbent such as those mentioned hereinbefore. Thus, using silica gel or a macroporous nonionic adsorbent resin and the above solvents, the desired compounds may be fractionally eluted. When, for example, silica gel is employed, development is carried out with hexane, ethyl acetate, or chloroform-methanol, ~hereby C-15003 P-4, P-3' and P-3 emerge in that order. After detect-ion by TLC, the fractions corresponding to C-15003 P-4, P-3' and P-3 are respect-lvely concentrated under reduced pressure and ethyl acetate is added to the concentrates. In this manner, the respective compounds can be obtained as cry-stals. Nhen a macroporous nonionic adsorbent resin is employed, gradient elution with a varying ratio of alcohol, ketone or ester to water may be utilized. For example, by the gradient elution method involvingthe use of 60% methanol-water and 95% methanol-water, with 5% sodium chloride added, C-15003 P-3, P-3' and P-4 merge in the order mentioned. After sampling and detection by TLC, each group of active fractions is concentrated under reduced pressure and crystallized from ethyl acetate. The isolated crystals include ethyl acetate as a solvent of crystallization and, after drying over phosphorus pentoxide at 70C for 8 hours, sho~ the following physical and chemical properties. ~Table 4).

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h h h ~ ~ h ~ N U) 3 h rl 4~ V) ~ 4 0 U) h ~ ~ h _ ¦ )_ Based on the above molecular formula shown above and the antimicro-bial and antitumor activity data given hereinafter, the present antibiotic was compared with the known groups of antibiotics. The literature search failed to locate any distinct group similar to Antibiotic C-150Q3. However, a search for substances that might give ultraviolet absorptions similar to those of the pre-sent antibiotic among component of plant and other naturally-occurring organic compounds led us to the maytanacine groups and, based on the molecular formulas involved, in particular, it was assumed that the antibiotic belongs to the maytana-cine group of compounds containing two~nitrogen atoms.
Maytanacine was obtained as a component of plant and was reported in Journal of American Chemical Society 97, 5294(1975). The mass spec-trum of maytanacine is as follows.
M+-~a~ M+-~a+b) 48s-CM3 485-CQ

(a)=H~0 + HNCO 0 ~b)-R-C-OH
The presence of m/e 485, 470 and 450 for C-15003 P-3, P-3' and P-4 canvinces us at once that these compounds have a skeletal structure identical ~ith that of maytanacine, differentiating them from maytanacine in the kind of acyl group in 3-position. It is thus clear that Antibiotic C-15003 is a novel compound. When C-15003 P-3, P-3' and P-4 were each degradated with alkali and analy~ed by gas chromatography for the liberated carboxylic acids, it was found that isobutyric acid, butyric acid and isovaleric acid were obtainable from C-15003 P-3, C-15003 P-3' and C-15003 P-4, respectively. Figure 1 shows the structures, based on the above data, of C-15003 P-3, P-.3' and P-4.

Figure 1 R

CH3O ~ CH3 g ~ P-3 -CO-CH <CH3 3 P-3' -CO-CH2-CH2-CH3 N ~ p_4 -CO-CH2-CH ~ CH3 Biological activity:
A) Antimicrobial activity:
~ Yith trypticase-soy agar (BBL) as an assay medium, the inhibitory concentrations against the microorganisms named below were investigated by the paper dîsc method. Thus, filter-paper discs (Toyo Seisakusho, thin-type, 8mm dia.~ each impregnated with 0.02mQ of a 300 ~g/mQ solution of C-15003 P-3, P-3' or P-4 were placed on plates respectively inoculated with the microorganisms named below to investigate the minimal inhibitory concentrations. The results showed that the antibiotics had no activity against the following microorganisms.
Escherichia coli, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Klebsiella pneumoniae, Serratia marcescens, Mycobacterium avium.
On the other hand, with agar plates containing the assay medium (3.5 g disodium hydrogen phosphate, 0.5 g monopotassium dihydrogen phosphate, 5 g yeast extract (Difco*), 10 g glucose, 15 g agar, 1000 mQ distilled water, pH 7.0), t~he gro~th inhibition against Talaromyces avellaneus ~as assayed. In this assay, the minimal inhibitory concentrations were 3 ~g/mQ for C-15003 P-3 and P-3', and 1.5 ~gtmQ for C-15003 P-4. Furthermore, the wild strain of Tetrahymena pyriformis ~`as an assay organism was cultivated on an assay medium ~composed of 20 g 11211~14 Proteose-peptone (Difco*), 1 g yeast extract~ 2 g glucose, 1000 mQ distilled water and 10 mQ 1 M-phosphate buffer pH 7.0) at 28C for 44 to 48 hours and the growth inhibitory activity of the antibiotic compounds against this particular microorganism was determined by the serial dilution method. Growth inhibition occurred at 1 ~g/mQ for C-15003 P-3 and P-3' and at 0.5 ~g/mQ for C-15003 P-4.
Anti-fungal activity is shown in Table 5. As seen from Table 5, C-15003 has growth inhibitory activity against microorganisms which cause plant diseases. The filter-paper discs impregnated with 0.02 mQ of a 1000 ~g/mQ solu-tion of C-15003 were placed on plates respectively inoculated with the microorgan_ isms as following Table 5.

*Tra~emark -1~-11'~1814 Table 5 Anti-microbial spectra Test organisms IFO medium hourInhibition number diameter Alternaria 7515 PSA* 48 38 kikuchiana Fusicladium levieri 6477 PSA* 90 68 Helminthosporium 5273 PSA* 48 55 sigmoideum var.
irregulare Pyricularia oryzae - PSA* 48 53 Elsinoe fa~cetti 8417 PSA* 90 55 Fusarium oxysporum - PSA* 48 20 f. cucumerinum Guignardia laricina 7888 PSA* 48 12 Cochlioborus miyabeanus 5277 PSA* 48 60 Dioporthe citri 9170 PSA* 48 55 Gibberella zeae 8850 PSA* 48 37 Sclerotinia sclerotiorum 9395 PSA* 90 65 Venturia pirina 6189 PSA* 48 50 Pellicularia sasakii 9253 PSA* 48 50 Pythium aphanidermatum 7030 PSA* 48 58 Botrytis cinerea - PSA* 48 48 Aspergillus niger 4066 PSA* 48 0 Penicillium chrysogenum 4626 PSA* 48 35 Rhizopus nigricans 6188 PSA* 48 25 Saccharomyces cerevisiae 0209 pSA* 48 0 Rhodotorula rubra 0907 PSA* 48 28 Trichophyton rubrum 5467 GB** 48 38 Trichop~yton 7522 GB** 48 38 mantagrophytes 1~18i4 Test organisms IF0 medium hour Inhibition number diameter Candida albicans 0583 GB** 48 0 Candida utilis 0619 GB** 48 0 Cryptococcus neoformans 0410 GB** 48 43 *PSA: Potato sucrose agar medium **GB: Glucose nutrient agar medium B) Antitumour activity The therapeutic effects of C-15003 P-3, P-3' and P-4 (dosed intraperitoneally for 9 consecutive days) upon P388 leukemia in mice (1 x 106 cells/animal, mouse, intraperitoneally transplanted) were investigated. The results showed that, in terms of life span-extending ratio, these compounds had an antitumour activity as high as 200% at the dose level of 0.00625 mg/kg/day.
C~ Toxicity In an acute toxicity test with mice as test animals, which involved intraperitoneal injections of C-15003 P-3, P-3' and P-4, all of these antibiotics sho~ed a LD50 value more than 0.313 mg/kg.
As mentioned hereinbefore, the present Antibiotic C-15003 has strong inhibitory activity against fungi and protozoa and, therefore, is of value as an antifungal or antiprotozoan agent. Furthermore, because Antibiotic C-15003 dis-plays a life span-extending action upon tumour-bearing mammalia~ animals (e.g.
mouse), it is also expected that the compound will be of use as an antitumour drug.
Antibiotic C-15003, as an antifungal and antiprotozoan agent, can be used with advantage for an assessment of the bacterial ecology in the soil, active sludge, animal body fluid or the like. Thus, when valuable bacteria are to be isolated from soil samples or when the actions of bacteria are to be eval-uated independently of those of fungi and protozoa in connection with the opera-tion and analysis of an active sludge system used in the treatment of waste water, as the present antibiotic may be utilized to obtain a selective growth of the bacterial flora without permitting growth of the concomitant fungi and protozoa in the specimen. In a typical instance, the sample is added to a liquid or solid medium and 0.1 mQ of a 10 to 100 ~g/mQ solution of the antibiotic in 1% methanol-water is added per mQ of the medium, which is then incubated.

The present Antibiotic C-15003 can also be used as an anti-microbial agent for the treatment of plant diseases caused by the microorganisms mentioned in the above Table 5.
In the typical application, Antibiotic C-15003 is used in a form of 1% methanolic aqueous solution containing 0.5 ~g/mQ - 5~g/mQ of the antibiotic.
For instance Antibiotic C-15003 may be used for the control of the reddish brown sheath rot, the blast, the Helminthosporium leaf spot and thesheath blight of rice plants.
It is thus apparent that C-15003 P-3, P-3' and P-4 are all novel compounds having the same skeletal structure and can be used also as intermedia-tes for the production of other phar~aceutically useful compounds. Thus, by way of deacylation reaction, C-15003 P-0 ~maytansinol) with a hydroxyl group in 3-position can be derived from the present antibiotic. In this connection, because the acyl group is in position beta to carbonyl, the conventional reductive hydrolysis reaction can be employed with advantage. Thus, by means of a complex metal hydride ~e.g. lithium aluminum hydride (LiAlH4) ) at a low temperature ~e.g. -20-0C~, the 0-ester bond in 3-position may be hydrolytically cleaved, ~ithout affecting other functional groups, e.g. carbonyl, epoxy, carbon-carbon double bonds, etc., to yield maytansinol. The physical and chemical data on this maytansinol sample thus obtained is in good agreement with the data given in Kupchan et al~lhe Journal of American Chemical Society 97, 5294-5295~1975) ).
The following examples are further illustrative but by no means limitative of the invention, wherein "part~s)"is based on weight unless other-wise noted and the relationship between "part~s)" and "part~s) by volume"
corresponds to that between "gram(s)" and "milliliter~s)", and "%" is based on "weight/volume" unless otherwise noted.
Example 11'~1814 Nocardia No. C 15003 ~IFO 13726; FERM 3992; ATCC 31281) as grown on a medium (yeast extract-malt extract agar) was used to inoculate a 200 parts by volume fermenter containing 40 parts by volume of a seed culture medium (2% gluc-OSeJ 3% soluble starch, 1% raw soybean mealJ 1% corn steep liquorJ 0.5% Polypepton*J
0.3% NaCQJ 0.5% CaC03, pH 7.0). The inoculated medium was incubated at 28C for 48 hours to obtain an inoculum. A 0.5 part by volume portion of the inoculum thus obtained was transferred to a 200 parts by volume fermenter containing 40 parts by ~olume of a fermentation medium composed of 5% dextrinJ 3% corn steep liquorJ 0.1%
Polypepton and 0.5% CaC03 (pH 7.0)J and cultivated at 28C for 90 hours to give inaculum (seed culture).
As determined by the serial dilution method using Tetrahymena pyriformis W as an assay organism and Antibiotic C-15003 P-3 as the standard sampleJ the above culture was found to have a titer of 25 '~g/m~.
Example 2 A 10 parts by volume portion of the inoculum (seed) obtained in Example 1 was transferred to a 2000 parts by volume fermenter containing 500 parts b~ volume of a seed culture medium ~same as above~ and incubated at 28C for 48 hours. A 500 parts by volume portion of the resultant culture was transferred toa 50QOO parts by volume tank of stainless steel containg 30000 parts by volume of seed culture medium and cultivated at 28CJ under aeration (30000 parts by volume/
min.~ J agitation ~280 r.p.m. (1/2DT) ) and internal pressure (1 kg/cm2~ to obtain a seed culture. This culture was used to seed a 200000 parts by volume tank of stainless steel containing 100JOOO parts by volume of a fermentation medium similar to the one used in Example 1 at an inoculation rate of 10%. The inoculated medium ~as incubated at 28CJ under aeration (lOOjOOO parts by pressure (1 kg/cm) ) for 90 hours. As determined by the same procedure as that described in Example 1J
the culture obtained above was found to have a titer of 25 ~g/mQ.
*Trademark -24-11'~,1814 Example 3 To 95,000 parts by volume of the culture obtained in Example 2 was added 2,000 parts of Hyflo-Super-Cel (Johns Manville, U.S.A.) and, after thorough mixing, the mixture was filtered on a pressure filter to obtain 85,000 parts by volume of filtrate and 32,000 parts of moist cells. The filtrate 85,000 parts by volume was stirred and extracted with 30,000 parts by volume of ethyl acetate.
This procedure was repeated once again. The ethyl acetate layers are pooled, washed twice with 30,000 parts by volume portions of water, dried by the addition of 500 parts of anhydrous sodium sulfate and concentrated under reduced pressure to 200 parts by volume. Petroleum ether was added to the concentrate and the resultant precipitate was recovered by filtration (53 parts). This crude product I was stirred with 100 parts by volume of ethyl acetate and the insolubles were filtered off. The filtrate was stirred with 10 parts of silica gel .~erck,West German~, 0.05-0.2 mm) and the ethyl acetate was removed under reduced pressure.
The residue was applied to the top of a silica gel column ~400 parts by volume).
Elution was carried out with 500 parts by volume of hexane, 500 parts by volume of hexane-ethyl acetate ~3:1), 500 parts by volume of hexane-ethyl acetate (1:1), 5QQ parts by volume of hexane-ethyl acetate ~1:3), 500 parts by volume of ethyl acetate and 1,000 parts by volume of ethyl acetate-methanol ~50:1), with the eluate being collected in 100 parts by volume fractions.
One part by volume portion of each fraction was concentrated to drynessJ and O.l part by volume of ethyl acetate was added to the concentrate to give a mixture. The mixture was spotted at 2.5 cm from the bottom edge of a silica gel-glass plate ~Merck, West Germany, 60 F 254 0.25 mm, 20 x 20) and developed for about 17 cm with a solvent system of ethyl acetate-methanol (19:1). After develo-pment, detection was carried out with ultraviolet light ~2537A).

The active fractions No. 23-No. 28 of RfO.6-0.65 were collected and concentrated under reduced pressure to about 20 parts by volume. To this concent-rate was added 150 parts by volume of petroleum ether to obtain 15 parts of a crude product II.
Example 4 Nith stirring, 32,000 parts of the moist cells obtained in Example 3 were extracted with 50,000 parts by volume of 70% acetone-water for 3 hours and, then, filtered on a pressure filter. The extraction with 50,000 parts by volume of 70% acetone-water and subsequent filtration was repeated once again. The filtrates ~ere pooled and the acetone was removed by concentration under reduced pressure. The resultant aqueous system was passed through a column of 5,000 parts by volume Diaion* HP-10 (Mitsubishi Kasei K.K.). The column was washed with 20,000 parts by volume of water and 50% aqueous methanol, followed by elution ~ith 15,000 parts by volume of 90% methanol-water. The eluate was concentrated under reduced pressure to 3,000 parts by volume and shaken with 3,000 parts by ~olume of water and 3,000 parts by volume of ethyl acetate. The above procedure ~as repeated once again. The ethyl acetate layers were combined, washed with water, dried by the addition of anhydrous sodium sulfate and concentrated under reduced pressure to 200 parts by volume. Following the addition of petroleum ether, the precipitate was recovered by filtration ~28 parts). The above product ~as purified by means of a column of silica gel to recover 8.0 parts of crude product II.
Example 5 In lO parts by volume of ethyl acetate was dissolved 1.5 parts of the crude product II obtained in Example 3 and the solution was stirred well with 4 parts of silica gel (Merck, ~est Germany, 0.05-0.2 mm). The ethyl acetate was removed under reduced pressure. The residue was applied to the top of a column ~f 30a parts by volume silica gel and the column was first ~ashed with 500 parts *Trademark -26-i4 by volume of chloroform and then eluted with 500 parts by volume of chloroform-methanol ~50:1), 500 parts by volume of chloroform-methanol ~20:1) and 500 parts by volume of chloroform-methanol (10:1). The eluate was collected in 25 parts by volume fractions.
A 0.5 part by volume portion of each fraction was concentrated under reduced pressure. To the concentrate was added 0.05 part by volume of ethyl ace-tate, and the mixture as a sample was subjected to silica gel thin layer chromato-graphy ~developing system: chloroform-methanol = 9:1~.
The fraction Nos. 39 and 40 absorbing at 2537 A in the zone of Rf 0.50-Q.60 were collected and concentrated to dryness under reduced pressure. To the residue was added 2 parts by volume of ethyl acetate and the mixture was allo~ed to stand, whereupon 0.150 part crystals of Antibiotic C-15003 were obtain-ed.
The above crystals of Antibiotic C-15003 (0.150 part) were dissolved in 15 parts by volume of methanol, followed by addition of 0.300 part of sodium chloride and 15 parts by volume of water. A column was packed with 200 parts by volume of Diaion* HP-10 (Mitsubishi Kasei K.K.)and calibrated with 600 parts by volume of 50% methanol-water containing 5% of NaCQ. The sample solution prepared above ~as passed through the column, and gradient elution was carried out using 2Q 1,5Q0 parts by volume of 60% methanol-water containing 5% NaCQ and 1,500 parts by volume of 95% methanol-water. The eluate was collected in 15 parts by volume fractions and each fraction was investigated by silica gel thin layer chromato-graphy. The fractions 145 to 153 contained C-15003 P-3, the fractions 167-180 contained C-15003 P-3' and P-4 and the fractions 185-190 contained C-15003 P-4.
Each group of fractions was concentrated and dissolved by the addi-tion of 50 parts by volume of water and 100 parts by volume of ethyl acetate.
The solution was shaken in a separatory funnel and the water layer was separated *Trademark -27-~Z~14 and, after ~ashing with two 50 parts by volume-portions of water, the ethyl acetate layer was dried over anhydrous sodium sulfate, concentrated and allowed to stand. In the above manner, crystals were obtained from each group of frac-tions. The crystals were collected by filtration and dried.
C-15003 P-3 0.070 part C-15003 P-3', P-4 0.018 part C-150Q3 P-4 0.015 part The mixed crystals of C-15003 P-3' and P-4 (0.018 part) were dissolved in 0.3 part by volume of ethyl acetate and spotted in a line at a dis-tance of 2.5 cm from the bottom edge of a silica gel glass plate ~Merck, West Germany~ Kieselgel 60 F254 0.25 mm, 20 x 20), followed by development with ethylacetate-methanol (19:1). After development to about 18 cm, the absorption band at Rf Q.68 ~P-4) and Rf 0.65 ~P-3') were scraped off and each was independently extracted twice with ethyl acetate containing a sm~ll amount of water. The resultant ethyl acetate extract was w~shed with water, dried over anhydrous sodium sulfate, concentrated under reduced pressure and allowed to stand.
O.OlO Part crystals of C-15003 P-4 and 0.003 part crystals of C-15003 P-3' were obtained from the fractions of Rf 0.68 and Rf 0.65, respective-ly.
Example 6 One thousand parts by volume of the cultuse of Example 2 was in-oculated into a 200,000 parts by volume tank of stainless steel containing 100, 000 parts by volume of aseed culture medium and the inoculated medium was incu-bated at 28C under aeration (100,000 parts by volume/min.) and agitation (200 r.p.m.~ for 48 hours to prepare a seed culture. This seed culture was trans-ferred to a 2,000,000 parts by volume tank of stainless steel containing 1,000,000 parts by volume of a fermentation medium similar to that used in Exam~le 1 at a transplantation rate of 10%. Cultivation was carried out at 28C under aeration ~1,000,000 parts by volume/min.), agitation (120 r.p.m. (1/3 DT) ) and internal pressure (1 kg/cm2) for 90 hours. The ~esultant culture was found to have a titer of 20 ~g/mQ as assayed by the assay procedure described in Example 1.
To 900,000 parts by volume of the above culture was added 900,000 parts by volume of acetone and, after an hour's stirring, 20,000 parts of Hyflo-Super-Cel (Johns-Manville, U.S.A.) was added. The mixture was further stirred and filtered on a pressure filter machine.
To 1,700,000 parts by volume of the resultant filtrate was added 500,000 parts by volume of water and, in a Podbielniak* ~Podbielniak, Inc.), the mixture was extracted with 1,000,000 parts by volume of ethyl acetate. The ethyl acetate layer was washed with water, dried by the addition of anhydrous sodium sulfate and concentrated under reduced pressure. To the concentrate was added petroleum ether and the resultant precipitate was recovered by filtration and dried. By the above procedure was obtained 68 parts of crude product I. There-after, as in Examples 3,4 and 5, this crude product was purified to obtain 9.5 parts Ofc-l5oo3 P-3, 0.300 part of C-15003 P-3' and 2.5 parts of C-15003 P-4.
Example 7 In 1 part by volume of tetrahydrofuran was dissolved 0.015 part of the Antibiotic C-15003 crystals obtained in Example 5 and after the solution was cooled to -5C, 0.012 part of lithium al D inum hydride was added. The mixture ~as allowed to stand for 2 hours. Following the addition of 0.5 part by volume of a 1% aqueous solutIon of H2SO4, the reaction mixture was extracted with 2 parts by volume of ethyl acetate. The ethyl acetate layer was washed with water, dried by the addition of anhydrous sodium sulfate and concentrated under reduced pressure. Preparative TLC with silica gel was carried out on the concentrate and *Trademark -29-il'Z1~14 the zone of Rf 0.25 to 0.3 was scraped off and extracted with ethyl acetate containing a small amount of water. The extract was washed with water, dried over anhydrous sodium sulfate and concentrated under reduced pressure, whereupon crystals separated. ~he crystals were recovered by filtration and dried. By the above procedure was obtained O.OlOpartof ~15003 P-0, melting point 174C.
Elemental analysis: Found C,59.65; H, 6.58; ~,5.02;

CQ, 6.51; calcd. for C28H37CQN208 C, 59.52; H, 6.60, N, 4.96; CQ, 6.27 IR: 1715, 1670, 1580~cm UV~nm~: 232~32750), 244~sh, 30850), 252~31650), 281~5750), 288~5700) In properties, this product is identical with maytansinol.

Claims (3)

THE EMBODIMENTS OF THE INVENTION IN WIIICH AN EXCLUSIVE
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:

1. A process for producing a compound of the formula:

(II) which comprises subjecting Antibiotic C-15003 of the general formula:

(I) , wherein R represents , -CO-CH2-CH2-CH3 or , to reductive hydrolysis.

2. A method as claimed in claim 1, wherein the reductive hydrolysis is conducted by employing a complex metal hydride.

3. A method as claimed in claim 2, wherein the complex metal hydride is lithium aluminum hydride.