CA1084860A - Proteines immobilisees a action biologique - Google Patents
Proteines immobilisees a action biologiqueInfo
- Publication number
- CA1084860A CA1084860A CA283,728A CA283728A CA1084860A CA 1084860 A CA1084860 A CA 1084860A CA 283728 A CA283728 A CA 283728A CA 1084860 A CA1084860 A CA 1084860A
- Authority
- CA
- Canada
- Prior art keywords
- solution
- support material
- biologically active
- reaction
- enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 25
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 25
- 102000004190 Enzymes Human genes 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 18
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 38
- 239000000463 material Substances 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 229940088598 enzyme Drugs 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 16
- 108090000526 Papain Proteins 0.000 claims description 11
- 239000004365 Protease Substances 0.000 claims description 11
- 102000004142 Trypsin Human genes 0.000 claims description 11
- 108090000631 Trypsin Proteins 0.000 claims description 11
- 229940055729 papain Drugs 0.000 claims description 11
- 235000019834 papain Nutrition 0.000 claims description 11
- 239000012588 trypsin Substances 0.000 claims description 11
- 102100026189 Beta-galactosidase Human genes 0.000 claims description 6
- 108010059881 Lactase Proteins 0.000 claims description 6
- 239000002262 Schiff base Substances 0.000 claims description 6
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 6
- 229940116108 lactase Drugs 0.000 claims description 6
- 150000004753 Schiff bases Chemical class 0.000 claims description 5
- 230000004071 biological effect Effects 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 239000012460 protein solution Substances 0.000 claims 1
- 229960001322 trypsin Drugs 0.000 claims 1
- 239000000969 carrier Substances 0.000 abstract description 11
- 150000004982 aromatic amines Chemical class 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 21
- 235000018102 proteins Nutrition 0.000 description 18
- 108010093096 Immobilized Enzymes Proteins 0.000 description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 150000001299 aldehydes Chemical class 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 239000000377 silicon dioxide Substances 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 6
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 5
- 230000008878 coupling Effects 0.000 description 5
- 238000010168 coupling process Methods 0.000 description 5
- 238000005859 coupling reaction Methods 0.000 description 5
- 150000004985 diamines Chemical class 0.000 description 5
- 229960000587 glutaral Drugs 0.000 description 5
- 230000000977 initiatory effect Effects 0.000 description 5
- 238000011068 loading method Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 4
- 150000001412 amines Chemical class 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 239000006087 Silane Coupling Agent Substances 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 238000006149 azo coupling reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000012412 chemical coupling Methods 0.000 description 2
- 238000006193 diazotization reaction Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 230000003134 recirculating effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 229910000077 silane Inorganic materials 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XOLBLPGZBRYERU-UHFFFAOYSA-N tin dioxide Chemical compound O=[Sn]=O XOLBLPGZBRYERU-UHFFFAOYSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 108010058683 Immobilized Proteins Proteins 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 108700040099 Xylose isomerases Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000005264 aryl amine group Chemical group 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- -1 p-phenylene- Chemical class 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/726,592 US4072566A (en) | 1976-09-27 | 1976-09-27 | Immobilized biologically active proteins |
| US726,592 | 1976-09-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1084860A true CA1084860A (fr) | 1980-09-02 |
Family
ID=24919239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA283,728A Expired CA1084860A (fr) | 1976-09-27 | 1977-07-29 | Proteines immobilisees a action biologique |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4072566A (fr) |
| JP (1) | JPS5341490A (fr) |
| CA (1) | CA1084860A (fr) |
| DE (1) | DE2740008A1 (fr) |
| FR (1) | FR2365581A1 (fr) |
| GB (1) | GB1532160A (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4210722A (en) * | 1977-11-14 | 1980-07-01 | Minnesota Mining And Manufacturing Company | Protein immobilizer |
| US4229536A (en) * | 1978-12-28 | 1980-10-21 | Uop Inc. | Process for preparing immobilized enzymes |
| DE3130606C2 (de) * | 1981-08-01 | 1985-03-21 | Rolf Dr. 8700 Würzburg Siegel | Verfahren zur Isolierung von an der Antikörperbildung beteiligten Zellen |
| US4539294A (en) * | 1982-09-30 | 1985-09-03 | Akzona Incorporated | Immobilization of proteins on polymeric supports |
| GB2208649A (en) * | 1987-08-17 | 1989-04-12 | Vnii Biotekhnologii | Immobilization of fungal beta -galactosidase on an inorganic carrier |
| DK427987A (da) * | 1987-08-17 | 1989-02-18 | Vnii Biotekhnologii | Fremgangsmaade til immobilisering af fungal beta-galactosidase |
| US5279558A (en) * | 1989-06-16 | 1994-01-18 | Science Incorporated | Fluid delivery apparatus with an additive |
| US5385546A (en) * | 1992-06-24 | 1995-01-31 | Science Incorporated | Mixing and delivering system |
| US5236418A (en) * | 1992-12-07 | 1993-08-17 | Science Incorporated | Fluid mixing and delivery apparatus |
| CA2156721C (fr) * | 1993-03-16 | 1999-06-01 | Thomas B. Okarma | Methode pour separer des facteurs selectionnes du sang entier ou de ses composantes |
| US5437861A (en) * | 1993-03-16 | 1995-08-01 | Applied Immune Sciences, Inc. | Removal of selected factors from whole blood or its components; and prevention and treatment of septic shock syndrome |
| ES2152121B1 (es) * | 1996-08-19 | 2001-08-16 | Univ De Cordoba Depto Quimica | Procedimiento de fabricacion de productos inorganicos activados utiles como soportes solidos para la inmovilizacion covalente de lipasas y otras enzimas y nuevos productos asi obtenidos. |
| US5998183A (en) * | 1997-07-07 | 1999-12-07 | Le Fevre; Gerard N. | Enzyme immobilization on a siliceous support with a polyaldehyde cross-linking agent |
| US6540936B1 (en) * | 2000-06-19 | 2003-04-01 | Toagosei Co., Ltd. | Aldehyde gas absorbent and process for absorbing aldehyde gas |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3556945A (en) * | 1968-02-05 | 1971-01-19 | Corning Glass Works | Enzyme stabilization |
| US3519538A (en) * | 1968-09-05 | 1970-07-07 | Corning Glass Works | Chemically coupled enzymes |
| US3669841A (en) * | 1970-02-11 | 1972-06-13 | Monsanto Co | Attachment of enzymes to siliceous materials |
| CH579109A5 (fr) * | 1973-02-22 | 1976-08-31 | Givaudan & Cie Sa | |
| US3873426A (en) * | 1973-07-02 | 1975-03-25 | Miles Lab | Insoluble enzymes |
| US3930951A (en) * | 1974-05-28 | 1976-01-06 | Corning Glass Works | Bonding enzymes to porous inorganic carriers |
| FR2306213A1 (fr) * | 1975-04-04 | 1976-10-29 | Rhone Poulenc Ind | Enzymes immobilisees |
| US3983000A (en) * | 1976-04-01 | 1976-09-28 | Corning Glass Works | Bonding proteins to inorganic supports |
-
1976
- 1976-09-27 US US05/726,592 patent/US4072566A/en not_active Expired - Lifetime
-
1977
- 1977-07-29 CA CA283,728A patent/CA1084860A/fr not_active Expired
- 1977-09-06 DE DE19772740008 patent/DE2740008A1/de not_active Withdrawn
- 1977-09-22 JP JP11447877A patent/JPS5341490A/ja active Pending
- 1977-09-23 GB GB39716/77A patent/GB1532160A/en not_active Expired
- 1977-09-26 FR FR7728893A patent/FR2365581A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE2740008A1 (de) | 1978-03-30 |
| FR2365581A1 (fr) | 1978-04-21 |
| FR2365581B1 (fr) | 1983-05-13 |
| US4072566A (en) | 1978-02-07 |
| GB1532160A (en) | 1978-11-15 |
| JPS5341490A (en) | 1978-04-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |