CA1031676A - Method and reagent for the enzyme kinetic determination of the concentration of a substrate - Google Patents

Method and reagent for the enzyme kinetic determination of the concentration of a substrate

Info

Publication number
CA1031676A
CA1031676A CA210,745A CA210745A CA1031676A CA 1031676 A CA1031676 A CA 1031676A CA 210745 A CA210745 A CA 210745A CA 1031676 A CA1031676 A CA 1031676A
Authority
CA
Canada
Prior art keywords
reagent
concentration
substrate
enzyme kinetic
kinetic determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA210,745A
Other languages
English (en)
French (fr)
Inventor
Wolfgang Gruber
Reinhard Muller-Matthesius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Roche Diagnostics GmbH
Original Assignee
Eppendorf Geraetebau Netheler and Hinz GmbH
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19742440011 external-priority patent/DE2440011C2/de
Application filed by Eppendorf Geraetebau Netheler and Hinz GmbH, Boehringer Mannheim GmbH filed Critical Eppendorf Geraetebau Netheler and Hinz GmbH
Application granted granted Critical
Publication of CA1031676A publication Critical patent/CA1031676A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/62Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving uric acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA210,745A 1973-10-04 1974-10-04 Method and reagent for the enzyme kinetic determination of the concentration of a substrate Expired CA1031676A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19732349819 DE2349819A1 (de) 1973-10-04 1973-10-04 Verfahren zur enzymkinetischen konzentrationsbestimmung eines substrats
DE19742440011 DE2440011C2 (de) 1974-08-21 Verfahren und Reagenz zur enzymkinetischen Konzentrationsbestimmung eines Substrates

Publications (1)

Publication Number Publication Date
CA1031676A true CA1031676A (en) 1978-05-23

Family

ID=25765898

Family Applications (1)

Application Number Title Priority Date Filing Date
CA210,745A Expired CA1031676A (en) 1973-10-04 1974-10-04 Method and reagent for the enzyme kinetic determination of the concentration of a substrate

Country Status (14)

Country Link
US (1) US3977944A (da)
JP (1) JPS5724119B2 (da)
BE (1) BE820736A (da)
CA (1) CA1031676A (da)
CH (1) CH614534A5 (da)
DD (1) DD113813A5 (da)
DE (1) DE2349819A1 (da)
FI (1) FI57782C (da)
FR (1) FR2257905B1 (da)
GB (1) GB1486474A (da)
HU (1) HU172778B (da)
IT (1) IT1030641B (da)
NL (1) NL181936C (da)
SE (1) SE414409B (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2558536B2 (de) * 1975-12-24 1979-07-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur kinetischen Substratbestimmung und Reagens zu seiner Durchführung
US4162194A (en) * 1976-02-13 1979-07-24 Beckman Instruments, Inc. Kinetic assay for acid phosphotase and composition therefore
US4218535A (en) * 1976-03-16 1980-08-19 Beckman Instruments, Inc. Determination of enzyme substrate concentration
US4159923A (en) * 1977-01-11 1979-07-03 Beckman Instruments, Inc. Kinetic assay for inorganic phosphates and composition therefore
US4120755A (en) * 1977-04-28 1978-10-17 Beckman Instruments, Inc. Kinetic method for determination of glucose concentrations with glucose dehydrogenase
FR2418462A1 (fr) * 1978-02-22 1979-09-21 Anvar Procede de dosage de substrats intervenant dans des reactions enzymatiques
IT1099355B (it) * 1978-10-12 1985-09-18 Sclavo Inst Sieroterapeut Composizione adatta alla determinazione in cinetica del glucosio
US4322496A (en) 1980-04-17 1982-03-30 Eastman Kodak Company Inhibition of lactate oxidase
US4318985A (en) * 1981-01-29 1982-03-09 Miles Laboratories, Inc. Method and device for detecting glucose concentration
IE820943L (en) * 1982-04-21 1983-10-21 Bartos Patent Dev Holding Serological investigations by complement fixation test
JPS59159796A (ja) * 1983-03-02 1984-09-10 Oriental Yeast Co Ltd 酵素活性の測定方法
DE3340709A1 (de) * 1983-11-10 1985-05-23 Boehringer Mannheim Gmbh, 6800 Mannheim Kompetitiver inhibitor fuer gk
DE3890267T1 (de) * 1987-04-10 1989-05-03 Univ Southern Australia Bestimmung von ionen in fluessigkeiten
FI81120C (fi) * 1988-09-26 1990-09-10 Kone Oy Foerfarande foer bestaemning av glukos ur biologiska vaetska samt reagensblandning foer tillaempning av foerfarandet.
SE466158B (sv) * 1989-04-25 1992-01-07 Migrata Uk Ltd Analysmetod foer glukosbestaemning i helblod
US5429930A (en) * 1989-07-21 1995-07-04 Bio-Research Products, Inc. Kinetic enzyme assay for determining the CO2 content of body fluids using PEP carboxylase with inhibitor
US20030068655A1 (en) * 2001-09-12 2003-04-10 Protiveris, Inc. Microcantilever apparatus and methods for detection of enzymes
SE523240C2 (sv) * 2001-12-12 2004-04-06 Akzo Nobel Nv Användning av hydroxyetylsubstituerad amin som korrosionsinhibitor i saltvattenhaltig miljö i oljefältsapplikationer
US7555933B2 (en) * 2006-08-01 2009-07-07 Thermo Fisher Scientific Inc. Method and software for detecting vacuum concentrator ends-of-runs
DE102016121553A1 (de) * 2016-11-10 2018-05-17 Technische Universität Darmstadt Kompetitiver Test eines Enzymsubstrates mit interner Kompensation der Enzymaktivität
CN114577745A (zh) * 2022-02-28 2022-06-03 深圳市乐土生物医药有限公司 一种检测亚甲基四氢叶酸脱氢酶2活性的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3627645A (en) * 1969-07-31 1971-12-14 Arequipa Foundation Method and reagents for the determination of nicotine adenine dinucleotide phosphate and of glutathione

Also Published As

Publication number Publication date
SE414409B (sv) 1980-07-28
BE820736A (fr) 1975-04-04
JPS5724119B2 (da) 1982-05-22
FI57782C (fi) 1980-10-10
US3977944A (en) 1976-08-31
DE2440011A1 (da) 1975-10-23
CH614534A5 (da) 1979-11-30
GB1486474A (en) 1977-09-21
AU7397574A (en) 1976-04-08
HU172778B (hu) 1978-12-28
JPS5084298A (da) 1975-07-08
DE2349819A1 (de) 1975-04-17
FI57782B (fi) 1980-06-30
DD113813A5 (da) 1975-06-20
NL181936C (nl) 1987-12-01
FI287674A (da) 1975-04-05
IT1030641B (it) 1979-04-10
DE2440011B1 (de) 1975-10-23
FR2257905A1 (da) 1975-08-08
NL7413057A (nl) 1975-04-08
SE7412391L (da) 1975-04-07
NL181936B (nl) 1987-07-01
FR2257905B1 (da) 1979-09-28

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