BRPI0918875A2 - Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura - Google Patents

Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura

Info

Publication number
BRPI0918875A2
BRPI0918875A2 BRPI0918875A BRPI0918875A BRPI0918875A2 BR PI0918875 A2 BRPI0918875 A2 BR PI0918875A2 BR PI0918875 A BRPI0918875 A BR PI0918875A BR PI0918875 A BRPI0918875 A BR PI0918875A BR PI0918875 A2 BRPI0918875 A2 BR PI0918875A2
Authority
BR
Brazil
Prior art keywords
microorganism
genetically modified
making
culture system
conversion
Prior art date
Application number
BRPI0918875A
Other languages
English (en)
Inventor
d lynch Michael
P Mercogliano Christopher
Original Assignee
Opx Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Opx Biotechnologies Inc filed Critical Opx Biotechnologies Inc
Publication of BRPI0918875A2 publication Critical patent/BRPI0918875A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MÉTODO PARA FAZER UM MICROORGANISMO GENETICAMENTE MODIFICADO, MICROORGANISMO E SISTEMA DE CULTURA. A presente invenção se refere a métodos, sistemas e composições, incluindo microorganismos geneticamente modificados, direcionados a obter redução de conversão microbiana de ácido 3-hidroxipropiônico (3-HP) para aldeídos de 3-HP. Em várias concretizações isto é obtido é obtido pela interrupção de genes particulares de aldeído desidrogenases, incluindo múltiplas deleções de genes. Entre os ácidos nucléicos específicos deletados em que a desejada redução de conversão é obtida estão aldA, aldB, puuC e usg do microorganismo E. Coli geneticamente modificado.
BRPI0918875A 2008-09-15 2009-09-15 Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura BRPI0918875A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9693708P 2008-09-15 2008-09-15
PCT/US2009/057058 WO2010031083A2 (en) 2008-09-15 2009-09-15 Methods, systems and compositions related to reduction of conversions of microbially produced 3-hydroxypropionic acid (3-hp) to aldehyde metabolites

Publications (1)

Publication Number Publication Date
BRPI0918875A2 true BRPI0918875A2 (pt) 2017-08-08

Family

ID=42005832

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0918875A BRPI0918875A2 (pt) 2008-09-15 2009-09-15 Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura

Country Status (4)

Country Link
US (2) US20130189787A1 (pt)
EP (1) EP2326709A4 (pt)
BR (1) BRPI0918875A2 (pt)
WO (1) WO2010031083A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010298004B2 (en) * 2009-09-27 2016-02-25 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
US8809027B1 (en) * 2009-09-27 2014-08-19 Opx Biotechnologies, Inc. Genetically modified organisms for increased microbial production of 3-hydroxypropionic acid involving an oxaloacetate alpha-decarboxylase
US20130122541A1 (en) * 2010-01-27 2013-05-16 The Regents Of The University Of Colorado Microorganism production of high-value chemical products, and related compositions, methods and systems
WO2012074818A2 (en) 2010-11-22 2012-06-07 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production
CA2849303C (en) 2011-09-30 2019-09-17 Novozymes, Inc. Dehydrogenase variants and polynucleotides encoding same
SG11201501013PA (en) 2012-08-10 2015-04-29 Opx Biotechnologies Inc Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112015008111A2 (pt) * 2012-10-11 2017-11-28 Univ Denmark Tech Dtu célula de levedura geneticamente modificada, e , método para a produção de 3hp.
WO2014085330A1 (en) 2012-11-30 2014-06-05 Novozymes, Inc. 3-hydroxypropionic acid production by recombinant yeasts
WO2014145096A1 (en) 2013-03-15 2014-09-18 Cindy Hoppe Flash evaporation for production purification and recovery
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
EP2970085B1 (en) 2013-03-15 2018-11-07 Cargill, Incorporated Recovery of 3-hydroxypropionic acid
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
WO2015010103A2 (en) 2013-07-19 2015-01-22 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
DK3027733T3 (en) 2013-07-31 2018-12-10 Novozymes As Preparation of 3-Hydroxypropionic Acid in Recombinant Yeast Expressing an Insect Aspartate-1 Decarboxylase
EP4148127A1 (en) 2014-06-11 2023-03-15 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
US20180265902A1 (en) 2015-08-24 2018-09-20 Novozymes A/S Beta-Alanine Aminotransferases For The Production of 3-Hydroxypropionic Acid
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞
MY193581A (en) 2017-02-21 2022-10-19 Univ Duke Compositions and methods for robust dynamic metabolic control
US11203744B2 (en) 2018-06-21 2021-12-21 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL129722A0 (en) * 1996-11-13 2000-02-29 Du Pont Method for the production of 1,3-propanediol by recombinant organisms
US6852517B1 (en) * 1999-08-30 2005-02-08 Wisconsin Alumni Research Foundation Production of 3-hydroxypropionic acid in recombinant organisms
JP4532116B2 (ja) * 2002-01-18 2010-08-25 ノボザイムズ エー／エス アラニン２，３−アミノムターゼ
US7826975B2 (en) * 2002-07-10 2010-11-02 The Penn State Research Foundation Method for redesign of microbial production systems
EP1532516A4 (en) * 2002-07-10 2007-05-23 Penn State Res Found METHOD FOR DETERMINING GENE KNOCKOUT STRATEGIES
MXPA05003382A (es) * 2002-10-04 2005-06-22 Du Pont Proceso para la produccion biologica de 1,3-propanodiol con alto rendimiento.
EP1706457B1 (en) * 2003-12-04 2012-02-01 Novozymes A/S Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase
US20070148749A1 (en) * 2004-03-26 2007-06-28 Shinzo Yasuda Process for producting 1,3-propanediol and or/3-hydroxypropionic acid
DE102005048818A1 (de) * 2005-10-10 2007-04-12 Degussa Ag Mikrobiologische Herstellung von 3-Hydroxypropionsäure
WO2008091627A2 (en) * 2007-01-22 2008-07-31 Genomatica, Inc. Methods and organisms for growth-coupled production of 3-hydroxypropionic acid
US8048624B1 (en) * 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
US20110144377A1 (en) * 2009-06-16 2011-06-16 E. I. Du Pont Nemours And Company Process for the biological production of 3-hydroxypropionic acid with high yield

Also Published As

Publication number Publication date
EP2326709A2 (en) 2011-06-01
US20130189787A1 (en) 2013-07-25
WO2010031083A2 (en) 2010-03-18
WO2010031083A3 (en) 2010-07-08
EP2326709A4 (en) 2013-01-23
US20150072399A1 (en) 2015-03-12

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]