BRPI0814568A2 - Método para geração de linhagens célula estáveis expressando níveis altos de uma proteína de nteresse - Google Patents

Método para geração de linhagens célula estáveis expressando níveis altos de uma proteína de nteresse

Info

Publication number
BRPI0814568A2
BRPI0814568A2 BRPI0814568-7A2A BRPI0814568A BRPI0814568A2 BR PI0814568 A2 BRPI0814568 A2 BR PI0814568A2 BR PI0814568 A BRPI0814568 A BR PI0814568A BR PI0814568 A2 BRPI0814568 A2 BR PI0814568A2
Authority
BR
Brazil
Prior art keywords
interest
protein
high levels
generation method
stable cell
Prior art date
Application number
BRPI0814568-7A2A
Other languages
English (en)
Inventor
Tara Kristen Crawford Mc Fadd
Meijia Yang
Original Assignee
Merck Serono Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Serono Sa filed Critical Merck Serono Sa
Publication of BRPI0814568A2 publication Critical patent/BRPI0814568A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
BRPI0814568-7A2A 2007-07-17 2008-07-16 Método para geração de linhagens célula estáveis expressando níveis altos de uma proteína de nteresse BRPI0814568A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96103807P 2007-07-17 2007-07-17
PCT/EP2008/059313 WO2009010534A1 (en) 2007-07-17 2008-07-16 Method for generating stable cell lines expressing high levels of a protein of interest

Publications (1)

Publication Number Publication Date
BRPI0814568A2 true BRPI0814568A2 (pt) 2014-10-21

Family

ID=39791299

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0814568-7A2A BRPI0814568A2 (pt) 2007-07-17 2008-07-16 Método para geração de linhagens célula estáveis expressando níveis altos de uma proteína de nteresse

Country Status (13)

Country Link
US (1) US20100184090A1 (pt)
EP (1) EP2183369A1 (pt)
JP (1) JP2010533483A (pt)
KR (1) KR20100044855A (pt)
CN (1) CN101755051A (pt)
AU (1) AU2008277632A1 (pt)
BR (1) BRPI0814568A2 (pt)
CA (1) CA2690939A1 (pt)
EA (1) EA017398B1 (pt)
IL (1) IL203196A0 (pt)
MX (1) MX2010000614A (pt)
WO (1) WO2009010534A1 (pt)
ZA (1) ZA200909120B (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11365410B2 (en) 2015-04-13 2022-06-21 President And Fellows Of Harvard College Production and monitoring of metabolites in cells
EP3441471A1 (en) * 2017-08-08 2019-02-13 CEVEC Pharmaceuticals GmbH Use of constitutively active variants of growth factor receptors as selection makers for the generation of stable producer cell lines
CN107699608A (zh) * 2017-11-22 2018-02-16 上海药明生物技术有限公司 利用染色体原位荧光杂交检验cho外源基因插入位点的方法
RU2768735C1 (ru) * 2020-12-31 2022-03-24 Федеральное государственное бюджетное образовательное учреждение высшего образования «Московский государственный университет имени М.В.Ломоносова» (МГУ) Способ получения стабильной клеточной линии-продуцента рекомбинантных белков
WO2022270946A1 (ko) * 2021-06-23 2022-12-29 한국생명공학연구원 세포내 면역 반응의 억제를 통한 목적 단백질의 발현 향상용 재조합 벡터 및 이의 응용

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7384744B2 (en) * 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products

Also Published As

Publication number Publication date
WO2009010534A1 (en) 2009-01-22
EA201070152A1 (ru) 2010-06-30
CN101755051A (zh) 2010-06-23
US20100184090A1 (en) 2010-07-22
AU2008277632A1 (en) 2009-01-22
EA017398B1 (ru) 2012-12-28
ZA200909120B (en) 2011-02-23
IL203196A0 (en) 2011-08-01
JP2010533483A (ja) 2010-10-28
MX2010000614A (es) 2010-04-22
CA2690939A1 (en) 2009-01-22
KR20100044855A (ko) 2010-04-30
EP2183369A1 (en) 2010-05-12

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 6A E 7A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]