BRPI0701529A2 - nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit - Google Patents
nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kitInfo
- Publication number
- BRPI0701529A2 BRPI0701529A2 BRPI0701529-1A BRPI0701529A BRPI0701529A2 BR PI0701529 A2 BRPI0701529 A2 BR PI0701529A2 BR PI0701529 A BRPI0701529 A BR PI0701529A BR PI0701529 A2 BRPI0701529 A2 BR PI0701529A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleotide sequences
- kit
- detection
- quantification
- quantifying
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Abstract
Padrão de Referência Contendo Sequência Nucleotídica, Processo para a Detecção e/ou Quantificação de Seqúências Nucleotídicas, e Kit. A presente invenção proporciona meios práticos e eficazes pelos quais sequências nucleotídicas de qualquer organismo podem ser utilizadas como padrões em sistemas de detecção e/ou quantificação, notadamente nos casos em que são empregadas técnicas de amplificação de ácidos nucléicos. Os padrões de referência da invenção são úteis na quantificação de seqúências nucleotidicas e/ou de organismos em tecidos, fluidos biológicos, alimentos e cosméticos, a partir do emprego da técnica de Reação de Polimerase em Cadeia, preferencialmente associada à fluorescência, ou PCR em Tempo Real. As aplicações da invenção incluem a geração de dados em pesquisa que envolva quantificação de ácidos nucléicos, incluindo DNA e/ou RNA, bem como dos organismos que os contêm, como patógenos virais ou bacterianos, sendo, portanto, útil em serviços de diagnóstico hospitalar para detecção e quantificação de patógenos em quadros de infecção e na indústria alimentícia e de cosméticos. A presente invenção também proporciona um melhorado processo para a detecção e/ou quantificação de seqúências nucleotídicas, e um kit.Reference Standard Containing Nucleotide Sequence, Process for Detecting and / or Quantifying Nucleotide Sequences, and Kit. The present invention provides practical and effective means by which nucleotide sequences from any organism can be used as standards in detection and / or quantification systems. , especially where nucleic acid amplification techniques are employed. The reference standards of the invention are useful in quantifying nucleotide sequences and / or organisms in tissues, biological fluids, food and cosmetics by employing the polymerase chain reaction, preferably fluorescence, or Time PCR technique. Real. Applications of the invention include research data generation involving quantification of nucleic acids, including DNA and / or RNA, as well as the organisms containing them, such as viral or bacterial pathogens, and is therefore useful in hospital diagnostic services for detection and quantification of pathogens in infection and in the food and cosmetics industry. The present invention also provides an improved method for detecting and / or quantifying nucleotide sequences, and a kit.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BRPI0701529-1A BRPI0701529A2 (en) | 2007-04-05 | 2007-04-05 | nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit |
PCT/BR2008/000091 WO2008122097A1 (en) | 2007-04-05 | 2008-04-04 | Synthesis of standards for detection and quantification of nucleic acids and kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BRPI0701529-1A BRPI0701529A2 (en) | 2007-04-05 | 2007-04-05 | nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0701529A2 true BRPI0701529A2 (en) | 2008-11-18 |
Family
ID=39830422
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0701529-1A BRPI0701529A2 (en) | 2007-04-05 | 2007-04-05 | nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit |
Country Status (2)
Country | Link |
---|---|
BR (1) | BRPI0701529A2 (en) |
WO (1) | WO2008122097A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201720088D0 (en) | 2017-12-01 | 2018-01-17 | Univ Oslo Hf | PCR Controls |
CN110408612B (en) * | 2019-07-09 | 2023-02-21 | 上海市计量测试技术研究院(中国上海测试中心、华东国家计量测试中心、上海市计量器具强制检定中心) | Protective agent for low-concentration DNA standard substance, preservation method and application |
CN112680534B (en) * | 2021-01-21 | 2022-10-28 | 哈尔滨医科大学 | Mycobacterium tuberculosis sRNA fluorescent quantitative PCR standard substance for identifying false positive reaction and application thereof |
CN117867086A (en) * | 2024-03-12 | 2024-04-12 | 北京雅康博生物科技有限公司 | Standard substance for quantitative high-throughput sequencing library and preparation method and application thereof |
-
2007
- 2007-04-05 BR BRPI0701529-1A patent/BRPI0701529A2/en not_active IP Right Cessation
-
2008
- 2008-04-04 WO PCT/BR2008/000091 patent/WO2008122097A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2008122097A1 (en) | 2008-10-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B03A | Publication of an application: publication of a patent application or of a certificate of addition of invention | ||
B06F | Objections, documents and/or translations needed after an examination request according art. 34 industrial property law | ||
B07D | Technical examination (opinion) related to article 229 of industrial property law | ||
B07G | Grant request does not fulfill article 229-c lpi (prior consent of anvisa) | ||
B08F | Application fees: dismissal - article 86 of industrial property law | ||
B08K | Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87) |