BR9908100A - Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a biosynthetic enzyme of carbohydrate in a host cell, method of obtaining a fragment of nucleic acid and product - Google Patents

Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a biosynthetic enzyme of carbohydrate in a host cell, method of obtaining a fragment of nucleic acid and product

Info

Publication number
BR9908100A
BR9908100A BR9908100-8A BR9908100A BR9908100A BR 9908100 A BR9908100 A BR 9908100A BR 9908100 A BR9908100 A BR 9908100A BR 9908100 A BR9908100 A BR 9908100A
Authority
BR
Brazil
Prior art keywords
nucleic acid
host cell
fragment
carbohydrate
chimeric gene
Prior art date
Application number
BR9908100-8A
Other languages
Portuguese (pt)
Inventor
Stephen M Allen
Willian D Hitz
Jonathan Edward Lightner
J Antoni Rafalski
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9908100A publication Critical patent/BR9908100A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"FRAGMENTO DE áCIDO NUCLéICO ISOLADO, GENE QUIMéRICO, CéLULA HOSPEDEIRA TRANSFORMADA, POLIPEPTìDEO, MéTODO DE ALTERAçãO DO NìVEL DE EXPRESSãO DE UMA ENZIMA BIOSSINTéTICA DE CARBOIDRATO EM UMA CéLULA HOSPEDEIRA, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO E PRODUTO" A presente invenção refere-se a fragmentos de ácido nucléicos isolados codificadores de uma enzima biossintética de carboidrato, selecionado a partir de sintases de 1,3-beta-D-glucano ou proteínas frágil-1 vegetais. A presente invenção também se refere à construção de uma gene quimérico que codifique a enzima biossintética de carboidrato, no todo ou em parte, em orientação de mesmo sentido ou sentido contrário, em que a expressão do gene quimérico resulte na produção de níveis alterados da enzima biossintética de carboidrato em uma célula hospedeira transformada."ISOLATED NUCLEIC ACID FRAGMENT, CHIMERIC GENE, TRANSFORMED HOSTED CELL, POLYPEPTIDE, METHOD OF CHANGING THE EXPRESSION LEVEL OF A CAREHYDRATIC ENTHUSIAST HOSHETIC PRODUCT, SYNTHETIC ENHYTE HOSTOPIC INVENTION" to isolated nucleic acid fragments encoding a carbohydrate biosynthetic enzyme, selected from 1,3-beta-D-glucan synthases or vegetable fragile-1 proteins. The present invention also relates to the construction of a chimeric gene that encodes the biosynthetic enzyme of carbohydrate, in whole or in part, in the same or opposite direction, in which the expression of the chimeric gene results in the production of altered levels of the enzyme carbohydrate biosynthesis in a transformed host cell.

BR9908100-8A 1998-03-26 1999-03-22 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a biosynthetic enzyme of carbohydrate in a host cell, method of obtaining a fragment of nucleic acid and product BR9908100A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7942098P 1998-03-26 1998-03-26
PCT/US1999/006583 WO1999049047A2 (en) 1998-03-26 1999-03-22 Plant 1,3-beta-d-glucan synthase and brittle-1 encoding sequences

Publications (1)

Publication Number Publication Date
BR9908100A true BR9908100A (en) 2001-09-04

Family

ID=22150434

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9908100-8A BR9908100A (en) 1998-03-26 1999-03-22 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a biosynthetic enzyme of carbohydrate in a host cell, method of obtaining a fragment of nucleic acid and product

Country Status (5)

Country Link
EP (1) EP1068324A2 (en)
AU (1) AU3204699A (en)
BR (1) BR9908100A (en)
CA (1) CA2319667A1 (en)
WO (1) WO1999049047A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6660850B2 (en) 1998-03-26 2003-12-09 E.I. Du Pont De Nemours And Company Nucleic acid encoding a wheat brittle-1 homolog
DE102004024184A1 (en) * 2004-05-13 2006-01-26 Basf Plant Science Gmbh Novel nucleic acid sequences and their use in methods for achieving pathogen resistance in plants
EP2140009A2 (en) * 2007-03-29 2010-01-06 CBD Technologies Ltd. Transgenic plants containing soluble cell wall polysaccharides

Also Published As

Publication number Publication date
CA2319667A1 (en) 1999-09-30
AU3204699A (en) 1999-10-18
EP1068324A2 (en) 2001-01-17
WO1999049047A2 (en) 1999-09-30
WO1999049047A3 (en) 2000-03-30

Similar Documents

Publication Publication Date Title
BR9910038A (en) Isolated nucleic acid fragment, chimeric gene, transformed polypeptide host cell, method of altering the level of expression of an enzyme, method of obtaining a nucleic acid fragment, product and method of evaluating at least one compound for its ability to inhibit the activity of an enzyme
BR9809967A (en) Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of an enzyme, method of obtaining a nucleic acid fragment, product and method for assessing the ability of at least one compound to inhibit the activity of an enzyme
WO2000032757A3 (en) Plant vitamin e biosynthetic enzymes
BR9815632A (en) Isolated nucleic acid fragment and method of obtaining it, chimeric gene, transformed host cell, method of altering the level of expression of an enzyme and product
DE69732628D1 (en) BETA 1-4 N-ACETYLGLUCOSAMINE TRANSFERASE AND THE GENE THAT CODES THEREOF
BR9908381A (en) Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, process of changing the level of expression of an enzyme, method of producing a desaturated fatty acid, seed, oil, method of producing seed oil, method of obtaining of a fragment of nucleic acid and product
WO1999010498A3 (en) Genes encoding enzymes for lignin biosynthesis and uses thereof
WO1994026906A3 (en) Cloned dna encoding a udp-galnac:polypeptide,n-acetylgalactos aminyltransferase
HUP0001743A2 (en) Soybean plant producing seeds with reduced levels of raffinose saccharides and phytic acid
WO1999061619A3 (en) Cell cycle genes, proteins and uses thereof
WO2004046336A3 (en) Production of increased oil and protein in plants by the disruption of the phenylpropanoid pathway
BR0115722A (en) Floral development genes
WO1999049013A3 (en) Tryptophan biosynthetic enzymes
BR9908100A (en) Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of a biosynthetic enzyme of carbohydrate in a host cell, method of obtaining a fragment of nucleic acid and product
BR9909414A (en) Isolated nucleic acid fragment encoding all or a substantial part of a flavanone-3-hydroxylase, chimeric gene, trans-formed host cell, flavanone-3-hydroxylase polypeptide, method of altering the level of expression of a flavanone-3-hydroxylase in a host cell, method of obtaining a nucleic acid fragment encoding all or a substantial part of the amino acid sequence encoding a flavanone-3-hydroxylase, its product and method of evaluating the ability of at least one compound to inhibit activity of a flavanone-3-hydroxylase
WO2001034817A3 (en) Genes encoding enzymes for lignin biosynthesis and uses thereof
BR9815578A (en) Nucleic acid fragment, chimeric gene, host cell, polypeptides, method of altering the level of expression of a biosynthetic enzyme, method of obtaining a nucleic acid fragment, and method of assessing the ability of at least one compound to inhibit activity of a biosynthetic enzyme
BR9813919A (en) Isolated nucleic acid fragment, chimeric gene, transformed host cell, sclb ^ r ^ polypeptide, method for altering the expression level of a sclb ^ r ^ protein, method for obtaining a nucleic acid fragment and product
BR9909605A (en) Isolated nucleic acid fragment encoding all or a substantial part of an acidic triacylglycerol lipase, chimeric gene, transformed host cell, acidic and neutral triacylglycerol polypeptide lipase, method of changing the level of expression of a triacylglycerol lipase in a host cell , method of obtaining a nucleic acid fragment encoding all or a substantial part of the amino acid sequence encoding a triacylglycerol lipase and product
WO2000037652A3 (en) Flavonoid biosynthetic enzymes
WO2000032792A3 (en) Plant 1-deoxy-d-xylulose 5-phosphate synthase
BR9815519A (en) Isolated nucleic acid fragment, chimeric gene, transformed host cell, method of altering the level of expression of a plant glycogenin, method of obtaining an isolated nucleic acid fragment and product
WO2000005386A3 (en) Chorismate biosynthesis enzymes
GT200000138CA (en) GENE OF STEPTOMYCES AVERMITILIS THAT DIRECTS THE RELATIONSHIP OF AVERMECTINES B1: B2 (FRACTIONARY APPLICATION NO.3, DERIVED FROM LAPATENT PI-2000-0138)
WO1999021880A3 (en) Plant branched-chain amino acid biosynthetic enzymes

Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
B11Y Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired