BR9907831A - Endo-xylogalacturonase - Google Patents
Endo-xylogalacturonaseInfo
- Publication number
- BR9907831A BR9907831A BR9907831-7A BR9907831A BR9907831A BR 9907831 A BR9907831 A BR 9907831A BR 9907831 A BR9907831 A BR 9907831A BR 9907831 A BR9907831 A BR 9907831A
- Authority
- BR
- Brazil
- Prior art keywords
- polypeptides
- endo
- xylogalacturonase
- new
- activity
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/231—Pectin; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01015—Polygalacturonase (3.2.1.15)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
Patente de Invenção:<B>"ENDO-XILOGALACTURONASE"<D>. São apresentados polipeptídios possuindo uma nova atividade, a saber, atividade de endo-xilogalacturonase. Estes polipeptídios podem degradar a pectina encontrada em extratos vegetais e materiais vegetais, e em particular nas regiões "pilosas" de polímeros de pectina. Em particular, os polipeptídios podem clivar em um polímero de ácido galacturónico em ligações glicosídicas internas. Está decrita a nova enzima XghA, e sua seq³ência de aminoácidos e a codificação da seq³ência de DNA dada. Este polipeptídio foi expresso em células de levedura e foi usado para tratar material vegetal, em particular suco de soja e de frutas na preparação de gêneros alimentícios comestíveis.Invention Patent: <B> "ENDO-XYLOGALACTURONASE" <D>. Polypeptides are shown to have a new activity, namely, endo-xylogalacturonase activity. These polypeptides can degrade the pectin found in plant extracts and plant materials, and in particular in the "hairy" regions of pectin polymers. In particular, polypeptides can cleave into a polymer of galacturonic acid at internal glycosidic bonds. The new XghA enzyme and its amino acid sequence and the coding of the given DNA sequence are being described. This polypeptide was expressed in yeast cells and was used to treat plant material, in particular soy and fruit juice in the preparation of edible foodstuffs.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98300952 | 1998-02-10 | ||
PCT/EP1999/000860 WO1999041386A2 (en) | 1998-02-10 | 1999-02-09 | Novel endo-xylogalacturonase |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9907831A true BR9907831A (en) | 2000-10-24 |
Family
ID=8234656
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9907831-7A BR9907831A (en) | 1998-02-10 | 1999-02-09 | Endo-xylogalacturonase |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1054978A2 (en) |
CN (1) | CN1294633A (en) |
AU (1) | AU2724799A (en) |
BR (1) | BR9907831A (en) |
WO (1) | WO1999041386A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011103812A1 (en) * | 2010-02-26 | 2011-09-01 | Novozymes A/S | Enzymatic pretreatment for making dried fruits |
CN102762104B (en) * | 2010-02-26 | 2014-08-06 | 诺维信公司 | Enzymatic pretreatment for making dried fruits |
WO2014170498A1 (en) | 2013-04-19 | 2014-10-23 | Danmarks Tekniske Universitet | Enzymatic production of polysaccharides from gum tragacanth |
CN107916268B (en) * | 2017-09-30 | 2021-10-22 | 武汉轻工大学 | Polygalacturonate lyase gene, recombinant expression vector, strain, polygalacturonate lyase and preparation method thereof |
CN114292832A (en) * | 2022-01-27 | 2022-04-08 | 大连海洋大学 | Thermophilic high-temperature-resistant polygalacturonaseMlPG28BEncoding gene and preparation method thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0421919A3 (en) * | 1989-09-02 | 1992-04-15 | Ciba-Geigy Ag | Polygalacturonase encoding fungal expression system |
JPH07503861A (en) * | 1992-12-24 | 1995-04-27 | ギスト ブロカデス ナムローゼ フェンノートシャップ | Cloning and expression of exo-polygalacturonase gene from Aspergillus |
AU2669795A (en) * | 1994-06-15 | 1996-01-05 | Novo Nordisk A/S | Extracts/cloud stability |
-
1999
- 1999-02-09 WO PCT/EP1999/000860 patent/WO1999041386A2/en not_active Application Discontinuation
- 1999-02-09 BR BR9907831-7A patent/BR9907831A/en not_active IP Right Cessation
- 1999-02-09 CN CN99804431A patent/CN1294633A/en active Pending
- 1999-02-09 EP EP99907529A patent/EP1054978A2/en not_active Withdrawn
- 1999-02-09 AU AU27247/99A patent/AU2724799A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1054978A2 (en) | 2000-11-29 |
CN1294633A (en) | 2001-05-09 |
WO1999041386A3 (en) | 2000-01-20 |
WO1999041386A2 (en) | 1999-08-19 |
AU2724799A (en) | 1999-08-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B25A | Requested transfer of rights approved |
Free format text: DSM IP ASSETS B.V. (NL) |
|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 9A E 10A ANUIDADES |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 1986 DE 27/03/2009. |
|
B15K | Others concerning applications: alteration of classification |
Ipc: A23L 2/84 (2006.01), A23L 29/00 (2016.0 |