BR9907831A - Endo-xylogalacturonase - Google Patents

Endo-xylogalacturonase

Info

Publication number
BR9907831A
BR9907831A BR9907831-7A BR9907831A BR9907831A BR 9907831 A BR9907831 A BR 9907831A BR 9907831 A BR9907831 A BR 9907831A BR 9907831 A BR9907831 A BR 9907831A
Authority
BR
Brazil
Prior art keywords
polypeptides
endo
xylogalacturonase
new
activity
Prior art date
Application number
BR9907831-7A
Other languages
Portuguese (pt)
Inventor
Petrus Johannes Alber Meeuwsen
Cecile Johanna Vlugt-Bergmans
Jean Paul Vincken
Gerrit Beldman
Alphons Gerard Joseph Voragen
Margareta Adriana Herweijer
Albert Johannes Joseph Ooijen
Original Assignee
Dsm Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv filed Critical Dsm Nv
Publication of BR9907831A publication Critical patent/BR9907831A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/231—Pectin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

Patente de Invenção:<B>"ENDO-XILOGALACTURONASE"<D>. São apresentados polipeptídios possuindo uma nova atividade, a saber, atividade de endo-xilogalacturonase. Estes polipeptídios podem degradar a pectina encontrada em extratos vegetais e materiais vegetais, e em particular nas regiões "pilosas" de polímeros de pectina. Em particular, os polipeptídios podem clivar em um polímero de ácido galacturónico em ligações glicosídicas internas. Está decrita a nova enzima XghA, e sua seq³ência de aminoácidos e a codificação da seq³ência de DNA dada. Este polipeptídio foi expresso em células de levedura e foi usado para tratar material vegetal, em particular suco de soja e de frutas na preparação de gêneros alimentícios comestíveis.Invention Patent: <B> "ENDO-XYLOGALACTURONASE" <D>. Polypeptides are shown to have a new activity, namely, endo-xylogalacturonase activity. These polypeptides can degrade the pectin found in plant extracts and plant materials, and in particular in the "hairy" regions of pectin polymers. In particular, polypeptides can cleave into a polymer of galacturonic acid at internal glycosidic bonds. The new XghA enzyme and its amino acid sequence and the coding of the given DNA sequence are being described. This polypeptide was expressed in yeast cells and was used to treat plant material, in particular soy and fruit juice in the preparation of edible foodstuffs.

BR9907831-7A 1998-02-10 1999-02-09 Endo-xylogalacturonase BR9907831A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98300952 1998-02-10
PCT/EP1999/000860 WO1999041386A2 (en) 1998-02-10 1999-02-09 Novel endo-xylogalacturonase

Publications (1)

Publication Number Publication Date
BR9907831A true BR9907831A (en) 2000-10-24

Family

ID=8234656

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9907831-7A BR9907831A (en) 1998-02-10 1999-02-09 Endo-xylogalacturonase

Country Status (5)

Country Link
EP (1) EP1054978A2 (en)
CN (1) CN1294633A (en)
AU (1) AU2724799A (en)
BR (1) BR9907831A (en)
WO (1) WO1999041386A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011103812A1 (en) * 2010-02-26 2011-09-01 Novozymes A/S Enzymatic pretreatment for making dried fruits
CN102762104B (en) * 2010-02-26 2014-08-06 诺维信公司 Enzymatic pretreatment for making dried fruits
WO2014170498A1 (en) 2013-04-19 2014-10-23 Danmarks Tekniske Universitet Enzymatic production of polysaccharides from gum tragacanth
CN107916268B (en) * 2017-09-30 2021-10-22 武汉轻工大学 Polygalacturonate lyase gene, recombinant expression vector, strain, polygalacturonate lyase and preparation method thereof
CN114292832A (en) * 2022-01-27 2022-04-08 大连海洋大学 Thermophilic high-temperature-resistant polygalacturonaseMlPG28BEncoding gene and preparation method thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0421919A3 (en) * 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
JPH07503861A (en) * 1992-12-24 1995-04-27 ギスト　ブロカデス　ナムローゼ　フェンノートシャップ Cloning and expression of exo-polygalacturonase gene from Aspergillus
AU2669795A (en) * 1994-06-15 1996-01-05 Novo Nordisk A/S Extracts/cloud stability

Also Published As

Publication number Publication date
EP1054978A2 (en) 2000-11-29
CN1294633A (en) 2001-05-09
WO1999041386A3 (en) 2000-01-20
WO1999041386A2 (en) 1999-08-19
AU2724799A (en) 1999-08-30

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Free format text: DSM IP ASSETS B.V. (NL)

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 9A E 10A ANUIDADES

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 1986 DE 27/03/2009.

B15K Others concerning applications: alteration of classification

Ipc: A23L 2/84 (2006.01), A23L 29/00 (2016.0