BR9907164A - Fragment of isolated nucleic acid, chimeric gene. host cell, transformed plant, polypeptides, method of altering the level of expression of a protein, method of obtaining a nucleic acid fragment, method of decreasing phosphoglycututase activity and the amount of starch in a developing soybean seed, seed plant and product - Google Patents

Fragment of isolated nucleic acid, chimeric gene. host cell, transformed plant, polypeptides, method of altering the level of expression of a protein, method of obtaining a nucleic acid fragment, method of decreasing phosphoglycututase activity and the amount of starch in a developing soybean seed, seed plant and product

Info

Publication number
BR9907164A
BR9907164A BR9907164-9A BR9907164A BR9907164A BR 9907164 A BR9907164 A BR 9907164A BR 9907164 A BR9907164 A BR 9907164A BR 9907164 A BR9907164 A BR 9907164A
Authority
BR
Brazil
Prior art keywords
nucleic acid
fragment
plant
expression
chimeric gene
Prior art date
Application number
BR9907164-9A
Other languages
Portuguese (pt)
Inventor
William D Hitz
Stephen M Allen
Florence Kirsch
J Antoni Rafalski
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9907164A publication Critical patent/BR9907164A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"FRAGMENTO DE áCIDO NUCLéICO ISOLADO, GENE QUIMéRICO, CéLULA HOSPEDEIRA, PLANTA TRANSFORMADA, POLIPEPTìDEOS, MéTODO DE ALTERAçãO DO NìVEL DE EXPRESSãO DE UMA PROTEìNA, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO, MéTODO PARA DIMINUIçãO DA ATIVIDADE DE FOSFOGLICOMUTASE E DA QUANTIDADE DE AMIDO EM UMA SEMENTE DE SOJA EM DESENVOLVIMENTO, SEMENTE DE PLANTA E PRODUTO" A presente invenção se refere a um fragmento de ácido nucléico isolado que codifica uma fosfoglucomutase. A invenção também se refere à construção de um gene quimérico que codifica toda ou uma parte de uma fosfoglucomutase, na orientação de sentido ou anti-sentido, sendo que a expressão do gene quimérico resulta na produção de níveis alterados de fosfoglucomutase em uma célula hospedeira transformada A invenção também se refere a plantas e sementes com níveis,reduzidos de amido como uma conseq³ência de expressão reduzida do gene que codifica a fosfoglucomutase."ISOLATED NUCLEIC ACID FRAGMENT, CHIMERIC GENE, HOSTING CELL, TRANSFORMED PLANT, POLYPEPTIMES, METHOD OF ALTERING THE EXPRESSION LEVEL OF A PROTEIN, METHOD OF OBTAINING A PHARMACEUTICAL DRAINAGE METHOD STARCH IN A DEVELOPING SOYBEAN, PLANT AND PRODUCT SEED "The present invention relates to an isolated nucleic acid fragment encoding a phosphoglucomutase. The invention also relates to the construction of a chimeric gene that encodes all or part of a phosphoglucomutase, in the sense or antisense orientation, the expression of the chimeric gene resulting in the production of altered levels of phosphoglucomutase in a transformed host cell The invention also relates to plants and seeds with reduced starch levels as a consequence of reduced expression of the gene encoding phosphoglucomutase.

BR9907164-9A 1998-01-15 1999-01-13 Fragment of isolated nucleic acid, chimeric gene. host cell, transformed plant, polypeptides, method of altering the level of expression of a protein, method of obtaining a nucleic acid fragment, method of decreasing phosphoglycututase activity and the amount of starch in a developing soybean seed, seed plant and product BR9907164A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7153298P 1998-01-15 1998-01-15
US11086698P 1998-12-04 1998-12-04
PCT/US1999/000883 WO1999036551A1 (en) 1998-01-15 1999-01-13 Plant phosphoglucomutase homologs

Publications (1)

Publication Number Publication Date
BR9907164A true BR9907164A (en) 2000-11-14

Family

ID=26752329

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9907164-9A BR9907164A (en) 1998-01-15 1999-01-13 Fragment of isolated nucleic acid, chimeric gene. host cell, transformed plant, polypeptides, method of altering the level of expression of a protein, method of obtaining a nucleic acid fragment, method of decreasing phosphoglycututase activity and the amount of starch in a developing soybean seed, seed plant and product

Country Status (3)

Country Link
AU (1) AU2321499A (en)
BR (1) BR9907164A (en)
WO (1) WO1999036551A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1268831A2 (en) * 2000-03-31 2003-01-02 Institut Für Pflanzengenetik Und Kulturpflanzenforschung Method for the production of leguminous plants with increased protein content and longer seed filling duration
US7323560B2 (en) 2000-07-17 2008-01-29 E.I. Du Pont De Nemours And Company Plastidic phosphoglucomutase genes
US7250557B2 (en) 2000-07-17 2007-07-31 E. I. Du Pont De Nemours And Company Plastidic phosphoglucomutase genes
WO2009143398A1 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Dgat genes from yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation for increased seed storage lipid production and altered fatty acid profiles in oilseed plants

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE118548T1 (en) * 1986-03-24 1995-03-15 Getty Scient Dev Co PRODUCTION PROCESS OF SUGAR NUCLEOTIDES USING RECOMBINANT PROCESS.
US5648210A (en) * 1991-07-24 1997-07-15 E. I. Du Pont De Nemours And Company Nucleotide sequences of galactinol synthase from zucchini and soybean
GB9307895D0 (en) * 1993-04-16 1993-06-02 Medical Res Council Mutation
CN1127016A (en) * 1993-05-28 1996-07-17 孟山都公司 Method of improving the quality of stored potatoes
GB9421287D0 (en) * 1994-10-21 1994-12-07 Danisco A method of reducing the level of sugar in an organism
BR9710158A (en) * 1996-07-09 1999-08-10 Unilever Nv Pea seeds pea plant process to extend to extend the harvest window or increase the sucrose content of a pea plant use of mutation rug3 sequence of polynucleotides vector process to alter one or more characteristics of a plant or part of the same altered plant or part of the same roots seeds fruits and other plant products
US6232529B1 (en) * 1996-11-20 2001-05-15 Pioneer Hi-Bred International, Inc. Methods of producing high-oil seed by modification of starch levels

Also Published As

Publication number Publication date
WO1999036551A1 (en) 1999-07-22
AU2321499A (en) 1999-08-02

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 3A, 4A, 5A, 6A, 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1910 DE 14/08/2007.