BR9808648A - Intron sense inhibition of starch branching enzyme expression - Google Patents

Intron sense inhibition of starch branching enzyme expression

Info

Publication number
BR9808648A
BR9808648A BR9808648-0A BR9808648A BR9808648A BR 9808648 A BR9808648 A BR 9808648A BR 9808648 A BR9808648 A BR 9808648A BR 9808648 A BR9808648 A BR 9808648A
Authority
BR
Brazil
Prior art keywords
intron
enzyme expression
branching enzyme
starch branching
nucleotide sequence
Prior art date
Application number
BR9808648-0A
Other languages
Portuguese (pt)
Inventor
Peter Poulsen
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9703672.7A external-priority patent/GB9703672D0/en
Priority claimed from GBGB9706075.0A external-priority patent/GB9706075D0/en
Application filed by Danisco filed Critical Danisco
Publication of BR9808648A publication Critical patent/BR9808648A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"INIBIçãO DE SENSO INTRON DA EXPRESSãO DE ENZIMA DE RAMIFICAçãO DE AMIDO" é descrito um método de inibição de expressão de gene. O método, que afeta a atividade enzimática em uma planta, compreende expressar em uma planta (ou célula, ou tecido ou órgão da mesma) uma seq³ência de nucleotídio onde a sequência de nucleotídio codifica, parcial ou completamente por uma classe de A SBE intron, com uma orientação de senso; e onde a seq³ência de nucleotídio não contém a seq³ência que tem senso a uma seq³ência exon normalmente associada com o intron."INTRON SENSE INHIBITION OF STARCH BRANCH ENZYME EXPRESSION" describes a method of inhibiting gene expression. The method, which affects enzyme activity in a plant, comprises expressing in a plant (or cell, or tissue or organ thereof) a nucleotide sequence where the nucleotide sequence encodes, partially or completely by a class of A SBE intron, with a sense orientation; and where the nucleotide sequence does not contain the sequence that makes sense for an exon sequence normally associated with the intron.

BR9808648-0A 1997-02-21 1998-02-23 Intron sense inhibition of starch branching enzyme expression BR9808648A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9703672.7A GB9703672D0 (en) 1997-02-21 1997-02-21 Inhibition of gene expression
GBGB9706075.0A GB9706075D0 (en) 1997-03-24 1997-03-24 Inhibition of gene expession
PCT/IB1998/000295 WO1998037214A1 (en) 1997-02-21 1998-02-23 Sense intron inhibition of starch branching enzyme expression

Publications (1)

Publication Number Publication Date
BR9808648A true BR9808648A (en) 2000-05-23

Family

ID=26311046

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9808648-0A BR9808648A (en) 1997-02-21 1998-02-23 Intron sense inhibition of starch branching enzyme expression

Country Status (8)

Country Link
EP (1) EP1007708A1 (en)
JP (1) JP2001512972A (en)
CN (1) CN1248293A (en)
AU (1) AU738311B2 (en)
BR (1) BR9808648A (en)
CA (1) CA2280210A1 (en)
NZ (1) NZ336607A (en)
WO (1) WO1998037214A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2826021B1 (en) * 2001-06-13 2004-12-31 Centre Nat Rech Scient METHOD FOR DETECTION AND IDENTIFICATION OF THE PRESENCE OF BIOLOGICAL MATERIALS FROM FISH, AND OLIGONUCLEOTIDES FOR IMPLEMENTING SAME
US7714186B2 (en) 2002-12-19 2010-05-11 Bayer Cropscience Ag Plant cells and plants which synthesize a starch with an increased final viscosity
FR2858631A1 (en) * 2004-09-24 2005-02-11 Centre Nat Rech Scient Method for detecting material from gadiform fish, useful for detecting adulteration of foods, based on amplification of mitochondrial DNA, also new oligonucleotides and amplicons
EP1707632A1 (en) 2005-04-01 2006-10-04 Bayer CropScience GmbH Phosphorylated waxy potato starch
CA2851563A1 (en) 2011-10-12 2013-04-18 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
WO2013053730A1 (en) 2011-10-12 2013-04-18 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
CN104017829A (en) * 2011-12-06 2014-09-03 中国科学院上海生命科学研究院 Method for increasing amylose content of plants
MX2018014496A (en) 2016-05-26 2019-03-28 Nunhems Bv Seedless fruit producing plants.
EP3806619A1 (en) 2018-06-15 2021-04-21 Nunhems B.V. Seedless watermelon plants comprising modifications in an abc transporter gene

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034323A (en) * 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
EP0575518A1 (en) * 1991-03-06 1993-12-29 Board Of Regents, The University Of Texas System Methods and compositions for the selective inhibition of gene expression
WO1996034968A2 (en) * 1995-05-05 1996-11-07 National Starch And Chemical Investment Holding Corporation Improvements in or relating to plant starch composition
GB9514437D0 (en) * 1995-07-14 1995-09-13 Danisco Inhibition of gene expression
GB9514435D0 (en) * 1995-07-14 1995-09-13 Danisco Inhibition of gene expression

Also Published As

Publication number Publication date
EP1007708A1 (en) 2000-06-14
WO1998037214A1 (en) 1998-08-27
NZ336607A (en) 2001-06-29
JP2001512972A (en) 2001-08-28
AU738311B2 (en) 2001-09-13
CN1248293A (en) 2000-03-22
AU6225998A (en) 1998-09-09
CA2280210A1 (en) 1998-08-27

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