BR9711645A - Método de superprodução de proteìna iniciada com um aminoácido diferente de metionina e proteìna produzida - Google Patents
Método de superprodução de proteìna iniciada com um aminoácido diferente de metionina e proteìna produzidaInfo
- Publication number
- BR9711645A BR9711645A BR9711645-9A BR9711645A BR9711645A BR 9711645 A BR9711645 A BR 9711645A BR 9711645 A BR9711645 A BR 9711645A BR 9711645 A BR9711645 A BR 9711645A
- Authority
- BR
- Brazil
- Prior art keywords
- protein
- amino acid
- overproduction
- methionine
- initiated
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 150000001413 amino acids Chemical class 0.000 title abstract 2
- 229930182817 methionine Natural products 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 title 1
- 238000012261 overproduction Methods 0.000 title 1
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 abstract 1
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 abstract 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 abstract 1
- 108010057757 methionyl-tRNA formyltransferase Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"MéTODO DE SUPERPRODUçãO DE PROTEìNA INICIADA COM UM AMINOáCIDO DIFERENTE DE METIONINA E PROTEìNA PRODUZIDA". Onde a transformilase de metionila-tRNA e, opcionalmente, a sintetase apropriada de tRNA são superexpressas em uma célula hospedeira.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/705,196 US5879905A (en) | 1996-08-29 | 1996-08-29 | In vivo method of initiating protein synthesis with an amino acid other than methionine |
PCT/US1997/015204 WO1998008959A1 (en) | 1996-08-29 | 1997-08-28 | In vivo protein synthesis method |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9711645A true BR9711645A (pt) | 2002-01-15 |
Family
ID=24832446
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9711645-9A BR9711645A (pt) | 1996-08-29 | 1997-08-28 | Método de superprodução de proteìna iniciada com um aminoácido diferente de metionina e proteìna produzida |
Country Status (9)
Country | Link |
---|---|
US (1) | US5879905A (pt) |
EP (1) | EP0927260A1 (pt) |
JP (1) | JP2000517186A (pt) |
CN (1) | CN1231697A (pt) |
AU (1) | AU719796B2 (pt) |
BR (1) | BR9711645A (pt) |
CA (1) | CA2264550A1 (pt) |
NZ (1) | NZ334879A (pt) |
WO (1) | WO1998008959A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6284878B1 (en) * | 1996-08-16 | 2001-09-04 | Smithkline Beecham Corporation | def1 |
US5962666A (en) * | 1997-09-16 | 1999-10-05 | Smithkline Beecham Corporation | Deformylase |
US6214602B1 (en) | 1998-08-28 | 2001-04-10 | Promega Corporation | Host cells for expression of clostridial toxins and proteins |
US6797820B2 (en) * | 1999-12-17 | 2004-09-28 | Vicuron Pharmaceuticals Inc. | Succinate compounds, compositions and methods of use and preparation |
WO2001055351A1 (en) * | 2000-01-28 | 2001-08-02 | Massachusetts Institute Of Technology | A eukaryotic cell expressing bacterial formylase and uses thereof |
US6586207B2 (en) * | 2000-05-26 | 2003-07-01 | California Institute Of Technology | Overexpression of aminoacyl-tRNA synthetases for efficient production of engineered proteins containing amino acid analogues |
MXPA06004087A (es) * | 2003-10-14 | 2006-06-27 | Scripps Research Inst | Incorporacion especifica de sitio de amino acidos redox activos en proteinas. |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5268284A (en) * | 1988-03-11 | 1993-12-07 | The Upjohn Company | Ribosome binding site |
-
1996
- 1996-08-29 US US08/705,196 patent/US5879905A/en not_active Expired - Fee Related
-
1997
- 1997-08-28 WO PCT/US1997/015204 patent/WO1998008959A1/en not_active Application Discontinuation
- 1997-08-28 CA CA002264550A patent/CA2264550A1/en not_active Abandoned
- 1997-08-28 JP JP10511937A patent/JP2000517186A/ja active Pending
- 1997-08-28 AU AU46879/97A patent/AU719796B2/en not_active Ceased
- 1997-08-28 NZ NZ334879A patent/NZ334879A/xx unknown
- 1997-08-28 CN CN97198313A patent/CN1231697A/zh active Pending
- 1997-08-28 EP EP97939642A patent/EP0927260A1/en not_active Withdrawn
- 1997-08-28 BR BR9711645-9A patent/BR9711645A/pt not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CA2264550A1 (en) | 1998-03-05 |
NZ334879A (en) | 2000-08-25 |
JP2000517186A (ja) | 2000-12-26 |
EP0927260A1 (en) | 1999-07-07 |
US5879905A (en) | 1999-03-09 |
AU4687997A (en) | 1998-03-19 |
WO1998008959A1 (en) | 1998-03-05 |
AU719796B2 (en) | 2000-05-18 |
CN1231697A (zh) | 1999-10-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FB36 | Technical and formal requirements: requirement - article 36 of industrial property law | ||
FA8 | Dismissal: dismissal - article 36, par. 1 of industrial property law |